                         SEQUENCE LISTING

<110>  University of Florida Research Foundation, Inc.
 
<120>  CHIMERIC ANTIGEN RECEPTOR T-CELLS EXPRESSING INTERLEUKIN-8 
       RECEPTOR

<130>  32917/53374 PC

<150>  US 62/555,251
<151>  2017-09-07

<150>  US 62/570,317
<151>  2017-10-20

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  2297
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human CD70CAR-CXCR1

<220>
<221>  misc_feature
<222>  (3)..(1103)
<223>  Extracellular CD27+Intracellular 41bb+CD3zeta; codon optimized

<220>
<221>  misc_feature
<222>  (113)..(1232)
<223>  Furin, V5 peptide, P2A ribosomal skip peptide

<220>
<221>  misc_feature
<222>  (1239)..(2291)
<223>  Human CXCR1; codon optimized

<400>  1
ccatggcaag accccacccc tggtggctgt gtgtgctggg cacactggtc ggactgagcg       60

ccacccctgc ccctaagagc tgccccgaga gacactactg ggctcagggc aagctgtgct      120

gccagatgtg cgagcccggc accttcctgg tgaaagactg cgaccagcac cggaaggccg      180

cccagtgcga tccttgcatc cccggcgtgt ccttcagccc cgaccaccac accagacccc      240

actgcgagag ctgccggcat tgcaactctg gcctgctggt ccgcaactgc accatcaccg      300

ccaacgccga gtgcgcctgc agaaacggct ggcagtgccg ggacaaagaa tgcaccgagt      360

gcgaccctct gcccaacccc agcctgaccg ccagaagcag ccaggctctg agccctcacc      420

ctcagcccac ccatctgccc tacgtgtccg agatgctgga agcccggaca gccggccaca      480

tgcagaccct ggccgacttc agacagctgc ccgccagaac cctgagcacc cactggcctc      540

cccagcggag cctgtgcagc agcgacttca tccggatcct ggtgatcttc agcggcatgt      600

tcctggtgtt caccctggct ggcgccctgt tcctgcacaa gcggggcaga aagaagctgc      660

tgtacatctt caagcagccc ttcatgcggc ccgtgcagac cacccaggaa gaggacggct      720

gcagctgccg gttccccgag gaagaggaag gcggctgcga gctgagagtg aagttcagca      780

gaagcgccga cgcccctgcc taccagcagg gccagaacca gctgtacaac gagctgaacc      840

tgggcagacg ggaagagtac gacgtgctgg acaagcggag aggccgggac cctgagatgg      900

gcggcaagcc ccagaggcgg aagaaccctc aggaaggcct gtataacgaa ctgcagaaag      960

acaagatggc cgaggcctac agcgagatcg gcatgaaggg cgagcggcgg agaggcaagg     1020

gccacgatgg cctgtaccag ggcctgagca ccgccaccaa ggacacctac gacgccctgc     1080

acatgcaggc tctgcctcca agattaatta accgtgccaa gcgaggtaag cctatcccta     1140

accctctcct cggtctcgat tctacgggat ccggagccac gaacttctct ctgttaaagc     1200

aagcaggaga cgtggaagaa aaccccggtc ctttcgaaat gagcaacatc acggacccgc     1260

agatgtggga cttcgacgac ctgaacttca ccggcatgcc tccagccgac gaggattaca     1320

gcccttgcat gctggaaacc gagacactga acaaatacgt ggtcatcatt gcctacgctc     1380

tggtgttcct gctgagcctg ctgggcaata gcctggtcat gctggtcatc ctgtacagca     1440

gagtgggcag atccgtgacc gacgtgtacc tgctgaatct ggccctggcc gatctgctgt     1500

ttgccctgac actgcctatt tgggccgcca gcaaagtgaa cggctggatc ttcggcacct     1560

tcctgtgcaa ggtggtgtct ctgctgaaag aagtgaactt ctacagcggc atcctgctgc     1620

tggcctgcat cagcgttgac agatacctgg ccatcgtgca cgccaccaga acactgaccc     1680

agaagagaca cctggtcaag ttcgtgtgcc tcggctgttg gggcctgagc atgaatctgt     1740

ctctgccatt cttcctgttc cggcaggcct accatcctaa caacagcagc cctgtgtgct     1800

acgaggtgct gggaaacgat acagccaagt ggcggatggt gctgcggatc ctgcctcaca     1860

ccttcggctt tatcgtgccc ctgttcgtga tgctgttctg ctacggcttc accctgcgga     1920

ccctgttcaa agcccacatg ggccagaaac accgggccat gagagtgatc tttgccgtgg     1980

tgctgatctt cctgctgtgc tggctgccct acaacctggt gctgcttgcc gacacactga     2040

tgcggaccca agtgatccaa gagagctgcg agcggcggaa caatatcggc agagccctgg     2100

atgctaccga gatcctgggc ttcctgcaca gctgtctgaa ccccatcatc tacgccttca     2160

tcggccagaa cttccggcac ggctttctga agattctggc catgcacggc ctggtgtcca     2220

aagagttcct ggccagacac agagtgacca gctacaccag cagctccgtg aacgtgtcca     2280

gcaacctctg agtcgac                                                    2297


<210>  2
<211>  2327
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  CD70CAR-CXCR2

<220>
<221>  misc_feature
<222>  (3)..(1103)
<223>  Extracellular CD27+Intracellular 41bb+CD3zeta; codon optimized

<220>
<221>  misc_feature
<222>  (1113)..(1232)
<223>  Furin, V5 peptide, P2A ribosomal skip peptide

<220>
<221>  misc_feature
<222>  (1239)..(2321)
<223>  Human CXCR2; codon optimized

<400>  2
ccatggcaag accccacccc tggtggctgt gtgtgctggg cacactggtc ggactgagcg       60

ccacccctgc ccctaagagc tgccccgaga gacactactg ggctcagggc aagctgtgct      120

gccagatgtg cgagcccggc accttcctgg tgaaagactg cgaccagcac cggaaggccg      180

cccagtgcga tccttgcatc cccggcgtgt ccttcagccc cgaccaccac accagacccc      240

actgcgagag ctgccggcat tgcaactctg gcctgctggt ccgcaactgc accatcaccg      300

ccaacgccga gtgcgcctgc agaaacggct ggcagtgccg ggacaaagaa tgcaccgagt      360

gcgaccctct gcccaacccc agcctgaccg ccagaagcag ccaggctctg agccctcacc      420

ctcagcccac ccatctgccc tacgtgtccg agatgctgga agcccggaca gccggccaca      480

tgcagaccct ggccgacttc agacagctgc ccgccagaac cctgagcacc cactggcctc      540

cccagcggag cctgtgcagc agcgacttca tccggatcct ggtgatcttc agcggcatgt      600

tcctggtgtt caccctggct ggcgccctgt tcctgcacaa gcggggcaga aagaagctgc      660

tgtacatctt caagcagccc ttcatgcggc ccgtgcagac cacccaggaa gaggacggct      720

gcagctgccg gttccccgag gaagaggaag gcggctgcga gctgagagtg aagttcagca      780

gaagcgccga cgcccctgcc taccagcagg gccagaacca gctgtacaac gagctgaacc      840

tgggcagacg ggaagagtac gacgtgctgg acaagcggag aggccgggac cctgagatgg      900

gcggcaagcc ccagaggcgg aagaaccctc aggaaggcct gtataacgaa ctgcagaaag      960

acaagatggc cgaggcctac agcgagatcg gcatgaaggg cgagcggcgg agaggcaagg     1020

gccacgatgg cctgtaccag ggcctgagca ccgccaccaa ggacacctac gacgccctgc     1080

acatgcaggc tctgcctcca agattaatta accgtgccaa gcgaggtaag cctatcccta     1140

accctctcct cggtctcgat tctacgggat ccggagccac gaacttctct ctgttaaagc     1200

aagcaggaga cgtggaagaa aaccccggtc ctttcgaaat ggaagatttc aacatggaaa     1260

gcgacagctt cgaggacttc tggaaaggcg aggacctgag caactacagc tacagctcta     1320

ccctgcctcc tttcctgctg gatgccgctc cttgtgaacc cgagagcctg gaaatcaaca     1380

agtacttcgt ggtcattatc tacgccctgg tgttcctgct gagcctgctg ggcaatagcc     1440

tggtcatgct ggtcatcctg tacagcagag tgggcagatc cgtgaccgac gtgtacctgc     1500

tgaatctggc cctggccgat ctgctgtttg ccctgacact gcctatttgg gccgccagca     1560

aagtgaacgg ctggatcttc ggcaccttcc tgtgcaaggt ggtgtctctg ctgaaagaag     1620

tgaacttcta ctccggcatc ctgctgctgg cctgcatcag cgttgacaga tacctggcca     1680

tcgtgcacgc caccagaaca ctgacccaga agcgctacct ggtcaagttc atctgcctga     1740

gcatctgggg actgtccctg ctgcttgctc tgcctgtgct gctgtttcgg agaaccgtgt     1800

acagctccaa cgtgtcccct gcctgctacg aggacatggg caacaacacc gccaactggc     1860

ggatgctgct gagaatcctg cctcagagct tcggcttcat cgtgcccctg ctgatcatgc     1920

tgttctgcta cggcttcacc ctgcggaccc tgttcaaagc ccacatgggc cagaaacacc     1980

gggccatgag agtgatcttt gccgtggtgc tgatcttcct gctgtgctgg ctgccctaca     2040

acctggtgct tctggccgac acactgatgc ggacccaagt gatccaagag acatgcgagc     2100

ggcggaacca catcgacaga gccctggatg ccacagagat cctgggaatc ctgcacagct     2160

gcctgaatcc tctgatctac gccttcatcg gacagaagtt ccggcacggc ctgctgaaga     2220

ttctggccat ccacggcctg atcagcaagg acagcctgcc taaggacagc agacctagct     2280

ttgtgggcag cagcagcggc cacacaagca ccacactttg agtcgac                   2327


<210>  3
<211>  1964
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human CD70CAR-EGFP

<220>
<221>  misc_feature
<222>  (3)..(1103)
<223>  Extracellular CD27+Intracellular 41bb+CD3zeta; codon optimized

<220>
<221>  misc_feature
<222>  (1113)..(1232)
<223>  Furin, V5 peptide, P2A ribosomal skip peptide

<220>
<221>  misc_feature
<222>  (1239)..(1958)
<223>  EGFP; codon optimized

<400>  3
ccatggcaag accccacccc tggtggctgt gtgtgctggg cacactggtc ggactgagcg       60

ccacccctgc ccctaagagc tgccccgaga gacactactg ggctcagggc aagctgtgct      120

gccagatgtg cgagcccggc accttcctgg tgaaagactg cgaccagcac cggaaggccg      180

cccagtgcga tccttgcatc cccggcgtgt ccttcagccc cgaccaccac accagacccc      240

actgcgagag ctgccggcat tgcaactctg gcctgctggt ccgcaactgc accatcaccg      300

ccaacgccga gtgcgcctgc agaaacggct ggcagtgccg ggacaaagaa tgcaccgagt      360

gcgaccctct gcccaacccc agcctgaccg ccagaagcag ccaggctctg agccctcacc      420

ctcagcccac ccatctgccc tacgtgtccg agatgctgga agcccggaca gccggccaca      480

tgcagaccct ggccgacttc agacagctgc ccgccagaac cctgagcacc cactggcctc      540

cccagcggag cctgtgcagc agcgacttca tccggatcct ggtgatcttc agcggcatgt      600

tcctggtgtt caccctggct ggcgccctgt tcctgcacaa gcggggcaga aagaagctgc      660

tgtacatctt caagcagccc ttcatgcggc ccgtgcagac cacccaggaa gaggacggct      720

gcagctgccg gttccccgag gaagaggaag gcggctgcga gctgagagtg aagttcagca      780

gaagcgccga cgcccctgcc taccagcagg gccagaacca gctgtacaac gagctgaacc      840

tgggcagacg ggaagagtac gacgtgctgg acaagcggag aggccgggac cctgagatgg      900

gcggcaagcc ccagaggcgg aagaaccctc aggaaggcct gtataacgaa ctgcagaaag      960

acaagatggc cgaggcctac agcgagatcg gcatgaaggg cgagcggcgg agaggcaagg     1020

gccacgatgg cctgtaccag ggcctgagca ccgccaccaa ggacacctac gacgccctgc     1080

acatgcaggc tctgcctcca agattaatta accgtgccaa gcgaggtaag cctatcccta     1140

accctctcct cggtctcgat tctacgggat ccggagccac gaacttctct ctgttaaagc     1200

aagcaggaga cgtggaagaa aaccccggtc ctttcgaaat ggtgagcaag ggagaggagc     1260

tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt     1320

tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca     1380

tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg     1440

gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg     1500

ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca     1560

agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg     1620

gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca     1680

gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga     1740

tccgccacaa catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc     1800

ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc     1860

tgagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg     1920

ccgggatcac tctcggcatg gatgagctgt acaagtaggt cgac                      1964


<210>  4
<211>  953
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human CXCR1

<400>  4
ccgagacact gaacaaatac gtggtcatca ttgcctacgc tctggtgttc ctgctgagcc       60

tgctgggcaa tagcctggtc atgctggtca tcctgtacag cagagtgggc agatccgtga      120

ccgacgtgta cctgctgaat ctggccctgg ccgatctgct gtttgccctg acactgccta      180

tttgggccgc cagcaaagtg aacggctgga tcttcggcac cttcctgtgc aaggtggtgt      240

ctctgctgaa agaagtgaac ttctacagcg gcatcctgct gctggcctgc atcagcgttg      300

acagatacct ggccatcgtg cacgccacca gaacactgac ccagaagaga cacctggtca      360

agttcgtgtg cctcggctgt tggggcctga gcatgaatct gtctctgcca ttcttcctgt      420

tccggcaggc ctaccatcct aacaacagca gccctgtgtg ctacgaggtg ctgggaaacg      480

atacagccaa gtggcggatg gtgctgcgga tcctgcctca caccttcggc tttatcgtgc      540

ccctgttcgt gatgctgttc tgctacggct tcaccctgcg gaccctgttc aaagcccaca      600

tgggccagaa acaccgggcc atgagagtga tctttgccgt ggtgctgatc ttcctgctgt      660

gctggctgcc ctacaacctg gtgctgcttg ccgacacact gatgcggacc caagtgatcc      720

aagagagctg cgagcggcgg aacaatatcg gcagagccct ggatgctacc gagatcctgg      780

gcttcctgca cagctgtctg aaccccatca tctacgcctt catcggccag aacttccggc      840

acggctttct gaagattctg gccatgcacg gcctggtgtc caaagagttc ctggccagac      900

acagagtgac cagctacacc agcagctccg tgaacgtgtc cagcaacctc tga             953


<210>  5
<211>  983
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human CXCR2

<400>  5
tggatgccgc tccttgtgaa cccgagagcc tggaaatcaa caagtacttc gtggtcatta       60

tctacgccct ggtgttcctg ctgagcctgc tgggcaatag cctggtcatg ctggtcatcc      120

tgtacagcag agtgggcaga tccgtgaccg acgtgtacct gctgaatctg gccctggccg      180

atctgctgtt tgccctgaca ctgcctattt gggccgccag caaagtgaac ggctggatct      240

tcggcacctt cctgtgcaag gtggtgtctc tgctgaaaga agtgaacttc tactccggca      300

tcctgctgct ggcctgcatc agcgttgaca gatacctggc catcgtgcac gccaccagaa      360

cactgaccca gaagcgctac ctggtcaagt tcatctgcct gagcatctgg ggactgtccc      420

tgctgcttgc tctgcctgtg ctgctgtttc ggagaaccgt gtacagctcc aacgtgtccc      480

ctgcctgcta cgaggacatg ggcaacaaca ccgccaactg gcggatgctg ctgagaatcc      540

tgcctcagag cttcggcttc atcgtgcccc tgctgatcat gctgttctgc tacggcttca      600

ccctgcggac cctgttcaaa gcccacatgg gccagaaaca ccgggccatg agagtgatct      660

ttgccgtggt gctgatcttc ctgctgtgct ggctgcccta caacctggtg cttctggccg      720

acacactgat gcggacccaa gtgatccaag agacatgcga gcggcggaac cacatcgaca      780

gagccctgga tgccacagag atcctgggaa tcctgcacag ctgcctgaat cctctgatct      840

acgccttcat cggacagaag ttccggcacg gcctgctgaa gattctggcc atccacggcc      900

tgatcagcaa ggacagcctg cctaaggaca gcagacctag ctttgtgggc agcagcagcg      960

gccacacaag caccacactt tga                                              983


<210>  6
<211>  1100
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  Human CD70 CAR

<400>  6
tggcaagacc ccacccctgg tggctgtgtg tgctgggcac actggtcgga ctgagcgcca       60

cccctgcccc taagagctgc cccgagagac actactgggc tcagggcaag ctgtgctgcc      120

agatgtgcga gcccggcacc ttcctggtga aagactgcga ccagcaccgg aaggccgccc      180

agtgcgatcc ttgcatcccc ggcgtgtcct tcagccccga ccaccacacc agaccccact      240

gcgagagctg ccggcattgc aactctggcc tgctggtccg caactgcacc atcaccgcca      300

acgccgagtg cgcctgcaga aacggctggc agtgccggga caaagaatgc accgagtgcg      360

accctctgcc caaccccagc ctgaccgcca gaagcagcca ggctctgagc cctcaccctc      420

agcccaccca tctgccctac gtgtccgaga tgctggaagc ccggacagcc ggccacatgc      480

agaccctggc cgacttcaga cagctgcccg ccagaaccct gagcacccac tggcctcccc      540

agcggagcct gtgcagcagc gacttcatcc ggatcctggt gatcttcagc ggcatgttcc      600

tggtgttcac cctggctggc gccctgttcc tgcacaagcg gggcagaaag aagctgctgt      660

acatcttcaa gcagcccttc atgcggcccg tgcagaccac ccaggaagag gacggctgca      720

gctgccggtt ccccgaggaa gaggaaggcg gctgcgagct gagagtgaag ttcagcagaa      780

gcgccgacgc ccctgcctac cagcagggcc agaaccagct gtacaacgag ctgaacctgg      840

gcagacggga agagtacgac gtgctggaca agcggagagg ccgggaccct gagatgggcg      900

gcaagcccca gaggcggaag aaccctcagg aaggcctgta taacgaactg cagaaagaca      960

agatggccga ggcctacagc gagatcggca tgaagggcga gcggcggaga ggcaagggcc     1020

acgatggcct gtaccagggc ctgagcaccg ccaccaagga cacctacgac gccctgcaca     1080

tgcaggctct gcctccaaga                                                 1100


<210>  7
<211>  120
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  fuV5P2A

<400>  7
cgtgccaagc gaggtaagcc tatccctaac cctctcctcg gtctcgattc tacgggatcc       60

ggagccacga acttctctct gttaaagcaa gcaggagacg tggaagaaaa ccccggtcct      120


<210>  8
<211>  620
<212>  DNA
<213>  Homo sapiens


<220>
<221>  misc_feature
<223>  EGFP

<400>  8
gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc       60

tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc      120

gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg      180

tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga      240

agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg      300

acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca      360

tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg      420

acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg      480

tgctgctgcc cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg      540

agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca      600

tggatgagct gtacaagtag                                                  620


