                         SEQUENCE LISTING

<110>  Monash University
 
<120>  Inhibition of C. difficile sporulation

<130>  53001859SCH

<150>  2017903760
<151>  2017-09-15

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  353
<212>  DNA
<213>  Clostridium difficile

<400>  1
aaaaaagctt ataattatcc ttagataacg atagagtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcgatttta tctcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt atgtctatcg acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  2
<211>  353
<212>  DNA
<213>  Clostridium difficile

<400>  2
aaaaaagctt ataattatcc ttagaagccg ccgaagtgcg cccagatagg gtgttaagtc       60

aagtagttta aggtactact ctgtaagata acacagaaaa cagccaacct aaccgaaaag      120

cgaaagctga tacgggaaca gagcacggtt ggaaagcgat gagttaccta aagacaatcg      180

ggtacgactg agtcgcaatg ttaatcagat ataaggtata agttgtgttt actgaacgca      240

agtttctaat ttcgattgct tctcgataga ggaaagtgtc tgaaacctct agtacaaaga      300

aaggtaagtt attttcggcg acttatctgt tatcaccaca tttgtacaat ctg             353


<210>  3
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

spoVDF1

<400>  3
aaaaaagctt ataattatcc tta                                               23


<210>  4
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

spoVDR

<400>  4
cagattgtac aaatgtggtg ataacagata agtc                                   34


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

01085F1 (pgtF1)

<400>  5
ggaattgaaa agagtatgga tag                                               23


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

01085R3 (pgtF1)

<400>  6
ttttatactt tcacatattt c                                                 21


<210>  7
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

spoVD_complementF_SphI

<400>  7
acatgacgca tgccatcaga ttgtatatgt ccacagcatt tacct                       45


<210>  8
<211>  37
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

spoVD_complementR_AatII

<400>  8
acatgcagac gtcctccaac tatggctaca aatgcat                                37


<210>  9
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

pgt_complementF_SphI

<400>  9
acatgacgca tgctagaagg tagaatgaag actgcatact atgt                        44


<210>  10
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Oligonucleotide

pgt_complementR_AatII

<400>  10
acatgcagac gtcatcctgt tatccatccg tggtctat                               38


<210>  11
<211>  659
<212>  PRT
<213>  Clostridium difficile

<400>  11

Met Arg Lys Val Lys Arg Ile Ser Lys Lys Arg Leu Val Leu Val Leu 
1               5                   10                  15      


Ile Leu Ala Cys Ala Leu Phe Phe Cys Leu Val Ile Arg Thr Gly Tyr 
            20                  25                  30          


Leu Gln Leu Met Lys Gly Asn Trp Leu Ser Thr Lys Ala Leu Glu Gln 
        35                  40                  45              


Gln Thr Arg Asp Ile Pro Ile Glu Pro Lys Arg Gly Thr Ile Tyr Asp 
    50                  55                  60                  


Arg Asn Met Lys Glu Leu Ala Val Ser Val Thr Lys Tyr Thr Val Trp 
65                  70                  75                  80  


Cys Lys Pro Val Glu Val Glu Asp Lys Lys Glu Ala Ala Glu Lys Val 
                85                  90                  95      


Ala Glu Ile Leu Asp Glu Asp Tyr Lys Asp Ile Tyr Ala Leu Ile Ser 
            100                 105                 110         


Lys Lys Asn Met Ala Leu Val Lys Val Lys Arg Trp Ile Asp Asp Asp 
        115                 120                 125             


Lys Ala Ser Gln Ile Arg Asp Ala Lys Leu Ser Gly Ile Trp Val Ala 
    130                 135                 140                 


Glu Asp Asn Gln Arg Tyr Tyr Pro Tyr Gly Asn Phe Ala Pro Tyr Val 
145                 150                 155                 160 


Leu Gly His Thr Ser Ser Asp Ala Thr Gly Ile Ser Gly Val Glu Met 
                165                 170                 175     


Gln Tyr Asp Lys Lys Leu Lys Gly Lys Pro Gly Lys Leu Ile Val Ser 
            180                 185                 190         


Thr Asp Ala Ser Gly Arg Glu Ile Pro Gln Gly Met Glu Lys Tyr Tyr 
        195                 200                 205             


Glu Pro Val Gln Gly Asn Gly Leu Val Leu Ser Ile Asp Glu Val Ile 
    210                 215                 220                 


Gln His Tyr Thr Glu Lys Ala Val Gln Lys Ala Tyr Glu Leu Asn Asn 
225                 230                 235                 240 


Ala Lys Lys Val Thr Ala Ile Ala Met Asn Pro Lys Thr Gly Asp Ile 
                245                 250                 255     


Leu Ala Leu Ala Ser Lys Pro Asp Tyr Asp Pro Asn Asp Ser Arg Thr 
            260                 265                 270         


Pro Ile Tyr Pro Tyr Tyr Gln Glu Glu Leu Glu Lys Tyr Asn Asp Lys 
        275                 280                 285             


Asp Lys Ile Lys Gly Tyr Tyr Gln Met Trp Arg Asn Pro Ala Val Ser 
    290                 295                 300                 


Asp Thr Tyr Glu Pro Gly Ser Thr Phe Lys Leu Ile Thr Ser Ser Ser 
305                 310                 315                 320 


Ala Leu Glu Glu Gly Val Ile Lys Asp Gly Glu Lys Phe Thr Cys Thr 
                325                 330                 335     


Gly Ser Val Thr Val Gly Gly Arg Lys Ile Lys Cys Trp Arg His Tyr 
            340                 345                 350         


Arg Pro His Gly Thr Gln Glu Phe Lys Gln Ala Val Gln Asn Ser Cys 
        355                 360                 365             


Asn Pro Val Phe Val Glu Leu Gly Ser Arg Leu Gly Val Gly Lys Met 
    370                 375                 380                 


Tyr Asp Tyr Ile Glu Ser Phe Gly Leu Met Asp Lys Thr Gly Ile Asp 
385                 390                 395                 400 


Leu Pro Gly Glu Ala Lys Gly Ile Leu Tyr Asn Glu Lys Asn Val Gly 
                405                 410                 415     


Pro Val Glu Leu Ala Thr Ile Ser Phe Gly Gln Ser Ile Ser Val Thr 
            420                 425                 430         


Pro Ile Gln Leu Ile Thr Ala Ile Ser Ser Ile Ala Asn Gly Gly Asp 
        435                 440                 445             


Leu Met Gln Pro Arg Val Val Lys Ser Tyr Thr Asp Asn Lys Gly Asn 
    450                 455                 460                 


Ile Thr Glu Thr Val Lys Pro Lys Lys Val Arg Ser Val Ile Ser Lys 
465                 470                 475                 480 


Glu Thr Ser Lys Lys Met Leu Glu Ile Ala Glu Ser Val Val Thr Glu 
                485                 490                 495     


Gly Gly Gly Lys Ile Ala Tyr Ile Pro Gly Tyr Arg Leu Gly Gly Lys 
            500                 505                 510         


Thr Gly Thr Ala Gln Lys Val Ile Asp Gly Lys Tyr Ala Pro Gly Lys 
        515                 520                 525             


Tyr Ile Cys Ser Phe Val Gly Ile Ala Pro Cys Asp Asp Pro Gln Ile 
    530                 535                 540                 


Val Val Leu Ala Ile Val Asp Glu Pro Thr Gly Val Ser Ala Phe Gly 
545                 550                 555                 560 


Ser Thr Thr Ala Gly Pro Ile Val Lys Glu Ile Met Asn Asp Ser Leu 
                565                 570                 575     


Lys Tyr Leu Gly Val Lys Pro Val Tyr Lys Glu Glu Glu Lys Ala Glu 
            580                 585                 590         


Tyr Glu Lys Lys Gln Val Lys Val Pro Asp Val Arg Asn Leu Lys Ile 
        595                 600                 605             


Gly Asp Ala Val Lys Ala Leu Glu Asp Ala Lys Leu Lys Pro Asp Leu 
    610                 615                 620                 


Asp Ala Asp Ile Glu Leu Pro Glu Asp Thr Lys Val Lys Asp Ile Phe 
625                 630                 635                 640 


Pro Lys Pro Gly Val Lys Val Asn Glu Asp Ser Ser Ile Thr Leu Tyr 
                645                 650                 655     


Phe Glu Asn 
            


<210>  12
<211>  554
<212>  PRT
<213>  Clostridium difficile

<400>  12

Met Ser Lys Lys Lys Thr Pro Phe Leu Lys Lys Val Gly Lys Arg Ser 
1               5                   10                  15      


Trp Cys Ile Phe Thr Ile Ile Leu Ile Ile Tyr Ser Val Leu Ile Tyr 
            20                  25                  30          


Arg Leu Val Asp Ile Gln Val Leu Lys Gly Asp Lys Tyr Lys Gln Ser 
        35                  40                  45              


Val Glu Ser Gln Ser Val Glu Lys Val Glu Leu Asn Ser Gly Arg Gly 
    50                  55                  60                  


Ile Ile Tyr Asp Arg Asn Asn Lys Lys Leu Thr Asp Thr Ser Lys Ser 
65                  70                  75                  80  


Gln Val Leu Ile Val Glu Lys Glu Lys Leu Asn Asn Asn Tyr Lys Ile 
                85                  90                  95      


Leu Glu Leu Ile Lys Lys Ala Thr Lys Met Asn Asp Leu Asp Ile Tyr 
            100                 105                 110         


Lys Ala Val Gln Glu Gln Leu Thr Arg Pro Ile Ile Gln Ile Gln Thr 
        115                 120                 125             


Lys Asn Ile Asp Lys Ser Met Lys Lys Glu Leu Glu Lys Asn Gly Ile 
    130                 135                 140                 


Met Val Glu Glu Lys Thr Met Arg Tyr Ala Lys Asp Gly Leu Leu Ser 
145                 150                 155                 160 


His Thr Ile Gly Tyr Ile Lys Glu Asp Asp Lys Ser Gly Gln Ser Gly 
                165                 170                 175     


Ile Glu Lys Ser Met Asp Ser Val Leu Arg Asn Ser Asn Glu Lys Tyr 
            180                 185                 190         


Ile Ser Ala Phe Lys Ala Gly Asp Ala Gly Asn Glu Lys Ser Leu Asn 
        195                 200                 205             


Ile Leu Lys Gly Ser Val Lys Thr Val Asp Asn Lys Asp Lys Asp Arg 
    210                 215                 220                 


His Leu Lys Thr Thr Ile Asp Tyr Asn Ile Gln Lys Lys Leu Glu Gln 
225                 230                 235                 240 


Ile Leu Asn Lys Glu Glu Asn Pro Thr Ala Ala Ile Ile Ser Glu Ala 
                245                 250                 255     


Ser Thr Gly Glu Ile Leu Ala Met Cys Ser Arg Pro Asn Phe Asp Gln 
            260                 265                 270         


Asn Asp Ile Ser Lys Ser Leu Lys Gly Lys Asn Gly Glu Phe Glu Asn 
        275                 280                 285             


Arg Val Ile Lys Ala Thr Tyr Pro Pro Gly Ser Val Phe Lys Met Val 
    290                 295                 300                 


Val Leu Phe Ser Ala Leu Glu Asn Gly Val Ile Asp Glu Asn Tyr Thr 
305                 310                 315                 320 


Tyr Asn Cys Thr Gly Lys Thr Lys Val Gly Asn Thr Asn Glu Ile Leu 
                325                 330                 335     


Arg Cys Asn Lys Arg Asp Gly His Gly Phe Gln Asn Leu Arg Gln Ala 
            340                 345                 350         


Phe Ser Asn Ser Cys Asn Pro Ala Phe Leu Asp Ile Ala Met Lys Leu 
        355                 360                 365             


Gly Lys Glu Lys Ile Leu Lys Ser Ala Glu Lys Leu His Leu Phe Glu 
    370                 375                 380                 


Lys Val Asp Ile Gly Leu Asp Glu Glu Lys Ile Arg Glu Ala Pro Lys 
385                 390                 395                 400 


Asn Ile Ser Ile Arg Asn Leu Ala Ile Gly Gln Glu Asn Ile Glu Phe 
                405                 410                 415     


Thr Pro Leu Gln Ile Asn Gln Met Thr Gln Ile Ile Ala Asn Asn Gly 
            420                 425                 430         


Thr Phe Lys Pro Leu Tyr Leu Tyr Lys Ser Leu Val Asp Asn Asn Met 
        435                 440                 445             


Asn Thr Ile Lys Thr Tyr Lys Ser Ser Lys Lys Glu Glu Leu Ile Ser 
    450                 455                 460                 


Pro Tyr Val Cys Thr Gln Val Lys Glu Tyr Met Lys Ser Val Ser Arg 
465                 470                 475                 480 


Ile Gly Thr Ala Lys Asp Leu Lys Asp Ile Glu Gly Gly Cys Gly Val 
                485                 490                 495     


Lys Thr Gly Thr Ala Gln Ser Ser Leu Asn Lys Lys Ala Ile Asp His 
            500                 505                 510         


Gly Trp Ile Thr Gly Phe Tyr Pro Glu Glu Arg Pro Lys Tyr Val Ile 
        515                 520                 525             


Thr Val Leu Val Glu Gly Thr Gln Lys Gly Asn Lys Ser Ala Thr Pro 
    530                 535                 540                 


Ile Phe Lys Glu Ile Cys Glu Ser Ile Lys 
545                 550                 


