                         SEQUENCE LISTING

<110>  Nature Technology Coropration
       Bozza, Matthias
       Harbottle, Richard
       Williams, James
 
<120>  NON-INTEGRATING DNA VECTORS FOR THE GENETIC MODIFICATION OF CELLS

<130>  NTC_PCT

<160>  29    

<170>  PatentIn version 3.5

<210>  1
<211>  281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R6K gamma origin

<400>  1
ggcttgttgt ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt       60

ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt      120

gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc      180

ttagtacgtt agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat      240

gagagcttag tacgtactat caacaggttg aactgctgat c                          281


<210>  2
<211>  281
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1 CpG R6K gamma origin

<400>  2
ggcttgttgt ccacaaccat taaaccttaa aagctttaaa agccttatat attctttttt       60

ttcttataaa acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt      120

gttcaaacat gagagcttag tacgtgaaac atgagagctt agtacattag ccatgagagc      180

ttagtacatt agccatgagg gtttagttca ttaaacatga gagcttagta cattaaacat      240

gagagcttag tacatactat caacaggttg aactgctgat c                          281


<210>  3
<211>  260
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG free R6K gamma origin

<400>  3
aaaccttaaa acctttaaaa gccttatata ttcttttttt tcttataaaa cttaaaacct       60

tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg agagcttagt      120

acatgaaaca tgagagctta gtacattagc catgagagct tagtacatta gccatgaggg      180

tttagttcat taaacatgag agcttagtac attaaacatg agagcttagt acatactatc      240

aacaggttga actgctgatc                                                  260


<210>  4
<211>  389
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Extended R6K gamma origin

<400>  4
tgtcagccgt taagtgttcc tgtgtcactg aaaattgctt tgagaggctc taagggcttc       60

tcagtgcgtt acatccctgg cttgttgtcc acaaccgtta aaccttaaaa gctttaaaag      120

ccttatatat tctttttttt cttataaaac ttaaaacctt agaggctatt taagttgctg      180

atttatatta attttattgt tcaaacatga gagcttagta cgtgaaacat gagagcttag      240

tacgttagcc atgagagctt agtacgttag ccatgagggt ttagttcgtt aaacatgaga      300

gcttagtacg ttaaacatga gagcttagta cgtgaaacat gagagcttag tacgtactat      360

caacaggttg aactgctgat cttcagatc                                        389


<210>  5
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNA-OUT selectable marker

<400>  5
gtagaattgg taaagagagt cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta       60

ttgatttttg gcgaaaccat ttgatcatat gacaagatgt gtatctacct taacttaatg      120

attttgataa aaatcatta                                                   139


<210>  6
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNA-OUT antisense repressor  RNA

<400>  6
tcgcacatct tgttgtctga ttattgattt ttggcgaaac catttgatca tatgacaaga       60

tgtgtatct                                                               69


<210>  7
<211>  139
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  2 CpG RNA-OUT selectable marker

<400>  7
gtagaattgg taaagagagt tgtgtaaaat attgagttcg cacatcttgt tgtctgatta       60

ttgatttttg gcgaaaccat ttgatcatat gacaagatgt gtatctacct taacttaatg      120

attttgataa aaatcatta                                                   139


<210>  8
<211>  466
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  R6K gamma origin -RNA-OUT bacterial replication-selection region 
       flanked by NheI and KpnI restriction  sites

<400>  8
gctagcccgc ctaatgagcg ggcttttttt tggcttgttg tccacaaccg ttaaacctta       60

aaagctttaa aagccttata tattcttttt tttcttataa aacttaaaac cttagaggct      120

atttaagttg ctgatttata ttaattttat tgttcaaaca tgagagctta gtacgtgaaa      180

catgagagct tagtacgtta gccatgagag cttagtacgt tagccatgag ggtttagttc      240

gttaaacatg agagcttagt acgttaaaca tgagagctta gtacgtacta tcaacaggtt      300

gaactgctga tccacgttgt ggtagaattg gtaaagagag tcgtgtaaaa tatcgagttc      360

gcacatcttg ttgtctgatt attgattttt ggcgaaacca tttgatcata tgacaagatg      420

tgtatctacc ttaacttaat gattttgata aaaatcatta ggtacc                     466


<210>  9
<211>  439
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  1 CpG R6K gamma origin - 2 CpG RNA-OUT bacterial 
       replication-selection region flanked by NheI and KpnI restriction
       sites

<400>  9
gctagctggc ttgttgtcca caaccattaa accttaaaag ctttaaaagc cttatatatt       60

cttttttttc ttataaaact taaaacctta gaggctattt aagttgctga tttatattaa      120

ttttattgtt caaacatgag agcttagtac gtgaaacatg agagcttagt acattagcca      180

tgagagctta gtacattagc catgagggtt tagttcatta aacatgagag cttagtacat      240

taaacatgag agcttagtac atactatcaa caggttgaac tgctgatctg tacagtagaa      300

ttggtaaaga gagttgtgta aaatattgag ttcgcacatc ttgttgtctg attattgatt      360

tttggcgaaa ccatttgatc atatgacaag atgtgtatct accttaactt aatgattttg      420

ataaaaatca ttaggtacc                                                   439


<210>  10
<211>  565
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-NP1 polylinker trpA R6K-RNA-OUT polylinker cloning cassette:
       EcoRI-HindIII

<400>  10
gaattcgagc tcggtacctc gcgaatgcat ctaggggacg gccgctagcc cgcctaatga       60

gcgggctttt ttttggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt      120

atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt      180

atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg      240

ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt      300

agtacgttaa acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatccacgt      360

tgtggtagaa ttggtaaaga gagtcgtgta aaatatcgag ttcgcacatc ttgttgtctg      420

attattgatt tttggcgaaa ccatttgatc atatgacaag atgtgtatct accttaactt      480

aatgattttg ataaaaatca ttaggagcta gcattgggtc atcggatccc gggcccgtcg      540

actgcagagg cctgcatgca agctt                                            565


<210>  11
<211>  584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-NP2 polylinker trpA R6K-RNA-OUT  polylinker cloning 
       cassette: EcoRI-HindIII

<400>  11
gaattcgagc tcggtacctc gcgaatgcat ctaggggacg gccgctagcc cgcctaatga       60

gcgggctttt ttttggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt      120

atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt      180

atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg      240

ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt      300

agtacgttaa acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatccacgt      360

tgtggtagaa ttggtaaaga gagtcgtgta aaatatcgag ttcgcacatc ttgttgtctg      420

attattgatt tttggcgaaa ccatttgatc atatgacaag atgtgtatct accttaactt      480

aatgattttg ataaaaatca ttaggactag tcccgggcgc tagttattaa tattgggtca      540

tcggatcccg ggcccgtcga ctgcagaggc ctgcatgcaa gctt                       584


<210>  12
<211>  557
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-NP3 polylinker trpA R6K-RNA-OUT  polylinker cloning 
       cassette: EcoRI-HindIII

<400>  12
gaattcgagc tcggtacctc gcgaatgcat ctaggggacg gccgctagcc cgcctaatga       60

gcgggctttt ttttggcttg ttgtccacaa ccgttaaacc ttaaaagctt taaaagcctt      120

atatattctt ttttttctta taaaacttaa aaccttagag gctatttaag ttgctgattt      180

atattaattt tattgttcaa acatgagagc ttagtacgtg aaacatgaga gcttagtacg      240

ttagccatga gagcttagta cgttagccat gagggtttag ttcgttaaac atgagagctt      300

agtacgttaa acatgagagc ttagtacgta ctatcaacag gttgaactgc tgatccacgt      360

tgtggtagaa ttggtaaaga gagtcgtgta aaatatcgag ttcgcacatc ttgttgtctg      420

attattgatt tttggcgaaa ccatttgatc atatgacaag atgtgtatct accttaactt      480

aatgattttg ataaaaatca ttaggtaccg agctcggatc ccgggcccgt cgactgcaga      540

ggcctgcatg caagctt                                                     557


<210>  13
<211>  557
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-NP4 polylinker trpA R6K-RNA-OUT  polylinker cloning 
       cassette: HindIII-EcoRI

<400>  13
aagcttgcat gcaggcctct gcagtcgacg ggcccgggat ccgagctcgg tacctcgcga       60

atgcatctag gggacggccg ctagcccgcc taatgagcgg gctttttttt ggcttgttgt      120

ccacaaccgt taaaccttaa aagctttaaa agccttatat attctttttt ttcttataaa      180

acttaaaacc ttagaggcta tttaagttgc tgatttatat taattttatt gttcaaacat      240

gagagcttag tacgtgaaac atgagagctt agtacgttag ccatgagagc ttagtacgtt      300

agccatgagg gtttagttcg ttaaacatga gagcttagta cgttaaacat gagagcttag      360

tacgtactat caacaggttg aactgctgat ccacgttgtg gtagaattgg taaagagagt      420

cgtgtaaaat atcgagttcg cacatcttgt tgtctgatta ttgatttttg gcgaaaccat      480

ttgatcatat gacaagatgt gtatctacct taacttaatg attttgataa aaatcattag      540

gtaccgagct cgaattc                                                     557


<210>  14
<211>  503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-NP5 polylinker trpA R6K-RNA-OUT  polylinker cloning 
       cassette: KasI-HindIII

<400>  14
ggcgccgcta gcccgcctaa tgagcgggct tttttttggc ttgttgtcca caaccgttaa       60

accttaaaag ctttaaaagc cttatatatt cttttttttc ttataaaact taaaacctta      120

gaggctattt aagttgctga tttatattaa ttttattgtt caaacatgag agcttagtac      180

gtgaaacatg agagcttagt acgttagcca tgagagctta gtacgttagc catgagggtt      240

tagttcgtta aacatgagag cttagtacgt taaacatgag agcttagtac gtactatcaa      300

caggttgaac tgctgatcca cgttgtggta gaattggtaa agagagtcgt gtaaaatatc      360

gagttcgcac atcttgttgt ctgattattg atttttggcg aaaccatttg atcatatgac      420

aagatgtgta tctaccttaa cttaatgatt ttgataaaaa tcattaggta ccacatgtcc      480

tgcagaggcc tgcatgcaag ctt                                              503


<210>  15
<211>  539
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-NP6 polylinker trpA R6K-RNA-OUT  polylinker cloning 
       cassette: EcoRI-SacI

<400>  15
gaattctgca gatatccatc acactggcgg ccgctagccc gcctaatgag cgggcttttt       60

tttggcttgt tgtccacaac cgttaaacct taaaagcttt aaaagcctta tatattcttt      120

tttttcttat aaaacttaaa accttagagg ctatttaagt tgctgattta tattaatttt      180

attgttcaaa catgagagct tagtacgtga aacatgagag cttagtacgt tagccatgag      240

agcttagtac gttagccatg agggtttagt tcgttaaaca tgagagctta gtacgttaaa      300

catgagagct tagtacgtac tatcaacagg ttgaactgct gatccacgtt gtggtagaat      360

tggtaaagag agtcgtgtaa aatatcgagt tcgcacatct tgttgtctga ttattgattt      420

ttggcgaaac catttgatca tatgacaaga tgtgtatcta ccttaactta atgattttga      480

taaaaatcat taggtaccgg gccccccctc gatcgaggtc gacggtatcg ggggagctc       539


<210>  16
<211>  500
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-NP7 polylinker trpA R6K-RNA-OUT  polylinker cloning 
       cassette: BssHII-BssHII

<400>  16
gcgcgcagcc ttaattaagc tagcccgcct aatgagcggg cttttttttg gcttgttgtc       60

cacaaccgtt aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa      120

cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg      180

agagcttagt acgtgaaaca tgagagctta gtacgttagc catgagagct tagtacgtta      240

gccatgaggg tttagttcgt taaacatgag agcttagtac gttaaacatg agagcttagt      300

acgtactatc aacaggttga actgctgatc cacgttgtgg tagaattggt aaagagagtc      360

gtgtaaaata tcgagttcgc acatcttgtt gtctgattat tgatttttgg cgaaaccatt      420

tgatcatatg acaagatgtg tatctacctt aacttaatga ttttgataaa aatcattagg      480

taccttaatt aactgcgcgc                                                  500


<210>  17
<211>  530
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pNTC-3xCpG NP1 polylinker R6K-RNA-OUT  polylinker cloning 
       cassette: HindIII-EcoRI

<400>  17
aagcttgcat gcaggcctct gcagtcgacg ggccctctag actcgagctg gcttgttgtc       60

cacaaccatt aaaccttaaa agctttaaaa gccttatata ttcttttttt tcttataaaa      120

cttaaaacct tagaggctat ttaagttgct gatttatatt aattttattg ttcaaacatg      180

agagcttagt acgtgaaaca tgagagctta gtacattagc catgagagct tagtacatta      240

gccatgaggg tttagttcat taaacatgag agcttagtac attaaacatg agagcttagt      300

acatactatc aacaggttga actgctgatc tgtacagtag aattggtaaa gagagttgtg      360

taaaatattg agttcgcaca tcttgttgtc tgattattga tttttggcga aaccatttga      420

tcatatgaca agatgtgtat ctaccttaac ttaatgattt tgataaaaat cattaggtac      480

cgctagcggc cgtcccctag atgcattcgc gaggtaccga gctcgaattc                 530


<210>  18
<211>  1973
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  human Interferon beta S/MAR flanked by 5' BglII-XhoI site and 3' 
       EcoRI restriction enzyme sites

<400>  18
agatctcgag ctcgatgata atgaatgtct aagttaatgc agaaacggag agacatacta       60

tattcatgaa ctaaaagact taatattgtg aaggtatact ttctttccac ataaatttgt      120

agtcaatatg ttcaccccaa aaaagctgtt tgttaacttg ccaacctcat tctaaaatgt      180

atatagaagc ccaaaagaca ataacaaaaa tattcttgta gaacaaaatg ggaaagaatg      240

ttccactaaa tatcaagatt tagagcaaag catgagatgt gtggggatag acagtgaggc      300

tgataaaata gagtagagct cagaaacaga cccattgata tatgtaagtg acctatgaaa      360

aaaatatggc attttacaat gggaaaatga tgatcttttt cttttttaga aaaacaggga      420

aatatattta tatgtaaaaa ataaaaggga acccatatgt cataccatac acacaaaaaa      480

attccagtga attataagtc taaatggaga aggcaaaact ttaaatcttt tagaaaataa      540

tatagaagca tgccatcatg acttcagtgt agagaaaaat ttcttatgac tcaaagtcct      600

aaccacaaag aaaagattgt taattagatt gcatgaatat taagacttat ttttaaaatt      660

aaaaaaccat taagaaaagt caggccatag aatgacagaa aatatttgca acaccccagt      720

aaagagaatt gtaatatgca gattataaaa agaagtctta caaatcagta aaaaataaaa      780

ctagacaaaa atttgaacag atgaaagaga aactctaaat aatcattaca catgagaaac      840

tcaatctcag aaatcagaga actatcattg catatacact aaattagaga aatattaaaa      900

ggctaagtaa catctgtggc aatattgatg gtatataacc ttgatatgat gtgatgagaa      960

cagtacttta ccccatgggc ttcctcccca aacccttacc ccagtataaa tcatgacaaa     1020

tatactttaa aaaccattac cctatatcta accagtactc ctcaaaactg tcaaggtcat     1080

caaaaataag aaaagtctga ggaactgtca aaactaagag gaacccaagg agacatgaga     1140

attatatgta atgtggcatt ctgaatgaga tcccagaaca gaaaaagaac agtagctaaa     1200

aaactaatga aatataaata aagtttgaac tttagttttt tttaaaaaag agtagcatta     1260

acacggcaaa gccattttca tatttttctt gaacattaag tacaagtcta taattaaaaa     1320

ttttttaaat gtagtctgga acattgccag aaacagaagt acagcagcta tctgtgctgt     1380

cgcctaacta tccatagctg attggtctaa aatgagatac atcaacgctc ctccatgttt     1440

tttgttttct ttttaaatga aaaactttat tttttaagag gagtttcagg ttcatagcaa     1500

aattgagagg aaggtacatt caagctgagg aagttttcct ctattcctag tttactgaga     1560

gattgcatca tgaatgggtg ttaaattttg tcaaatgctt tttctgtgtc tatcaatatg     1620

accatgtgat tttcttcttt aacctgttga tgggacaaat tacgttaatt gattttcaaa     1680

cgttgaacca cccttacata tctggaataa attctacttg gttgtggtgt atattttttg     1740

atacattctt ggattctttt tgctaatatt ttgttgaaaa tgtttgtatc tttgttcatg     1800

agagatattg gtctgttgtt ttcttttctt gtaatgtcat tttctagttc cggtattaag     1860

gtaatgctgg cctagttgaa tgatttagga agtattccct ctgcttctgt cttctgaaag     1920

agattgtaga aagttgatac aatttttttt tctttaaata tttgatagaa ttc            1973


<210>  19
<211>  2085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splice donor-human Interferon beta S/MAR-splice acceptor flanked 
       by 5' BglII site and 3' BamHI restriction enzyme sites

<400>  19
agatctatgc atgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa       60

gacaggtctc gagctcgatg ataatgaatg tctaagttaa tgcagaaacg gagagacata      120

ctatattcat gaactaaaag acttaatatt gtgaaggtat actttctttc cacataaatt      180

tgtagtcaat atgttcaccc caaaaaagct gtttgttaac ttgccaacct cattctaaaa      240

tgtatataga agcccaaaag acaataacaa aaatattctt gtagaacaaa atgggaaaga      300

atgttccact aaatatcaag atttagagca aagcatgaga tgtgtgggga tagacagtga      360

ggctgataaa atagagtaga gctcagaaac agacccattg atatatgtaa gtgacctatg      420

aaaaaaatat ggcattttac aatgggaaaa tgatgatctt tttctttttt agaaaaacag      480

ggaaatatat ttatatgtaa aaaataaaag ggaacccata tgtcatacca tacacacaaa      540

aaaattccag tgaattataa gtctaaatgg agaaggcaaa actttaaatc ttttagaaaa      600

taatatagaa gcatgccatc atgacttcag tgtagagaaa aatttcttat gactcaaagt      660

cctaaccaca aagaaaagat tgttaattag attgcatgaa tattaagact tatttttaaa      720

attaaaaaac cattaagaaa agtcaggcca tagaatgaca gaaaatattt gcaacacccc      780

agtaaagaga attgtaatat gcagattata aaaagaagtc ttacaaatca gtaaaaaata      840

aaactagaca aaaatttgaa cagatgaaag agaaactcta aataatcatt acacatgaga      900

aactcaatct cagaaatcag agaactatca ttgcatatac actaaattag agaaatatta      960

aaaggctaag taacatctgt ggcaatattg atggtatata accttgatat gatgtgatga     1020

gaacagtact ttaccccatg ggcttcctcc ccaaaccctt accccagtat aaatcatgac     1080

aaatatactt taaaaaccat taccctatat ctaaccagta ctcctcaaaa ctgtcaaggt     1140

catcaaaaat aagaaaagtc tgaggaactg tcaaaactaa gaggaaccca aggagacatg     1200

agaattatat gtaatgtggc attctgaatg agatcccaga acagaaaaag aacagtagct     1260

aaaaaactaa tgaaatataa ataaagtttg aactttagtt ttttttaaaa aagagtagca     1320

ttaacacggc aaagccattt tcatattttt cttgaacatt aagtacaagt ctataattaa     1380

aaatttttta aatgtagtct ggaacattgc cagaaacaga agtacagcag ctatctgtgc     1440

tgtcgcctaa ctatccatag ctgattggtc taaaatgaga tacatcaacg ctcctccatg     1500

ttttttgttt tctttttaaa tgaaaaactt tattttttaa gaggagtttc aggttcatag     1560

caaaattgag aggaaggtac attcaagctg aggaagtttt cctctattcc tagtttactg     1620

agagattgca tcatgaatgg gtgttaaatt ttgtcaaatg ctttttctgt gtctatcaat     1680

atgaccatgt gattttcttc tttaacctgt tgatgggaca aattacgtta attgattttc     1740

aaacgttgaa ccacccttac atatctggaa taaattctac ttggttgtgg tgtatatttt     1800

ttgatacatt cttggattct ttttgctaat attttgttga aaatgtttgt atctttgttc     1860

atgagagata ttggtctgtt gttttctttt cttgtaatgt cattttctag ttccggtatt     1920

aaggtaatgc tggcctagtt gaatgattta ggaagtattc cctctgcttc tgtcttctga     1980

aagagattgt agaaagttga tacaattttt ttttctttaa atatttgata gaattcttac     2040

tgacatccac tttgcctttc tctccacagg tgtccactcg gatcc                     2085


<210>  20
<211>  2085
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splice donor-human Interferon beta S/MAR (-AATAAA)-splice 
       acceptor flanked by 5' BglII site and 3' BamHI restriction enzyme
       sites

<400>  20
agatctatgc atgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa       60

gacaggtctc gagctcgatg ataatgaatg tctaagttaa tgcagaaacg gagagacata      120

ctatattcat gaactaaaag acttaatatt gtgaaggtat actttctttc cacataaatt      180

tgtagtcaat atgttcaccc caaaaaagct gtttgttaac ttgccaacct cattctaaaa      240

tgtatataga agcccaaaag acaataacaa aaatattctt gtagaacaaa atgggaaaga      300

atgttccact aaatatcaag atttagagca aagcatgaga tgtgtgggga tagacagtga      360

ggctgataaa atagagtaga gctcagaaac agacccattg atatatgtaa gtgacctatg      420

aaaaaaatat ggcattttac aatgggaaaa tgatgatctt tttctttttt agaaaaacag      480

ggaaatatat ttatatgtaa aaaatatttg ggaacccata tgtcatacca tacacacaaa      540

aaaattccag tgaattataa gtctaaatgg agaaggcaaa actttaaatc ttttagaaaa      600

taatatagaa gcatgccatc atgacttcag tgtagagaaa aatttcttat gactcaaagt      660

cctaaccaca aagaaaagat tgttaattag attgcatgaa tattaagact tatttttaaa      720

attaaaaaac cattaagaaa agtcaggcca tagaatgaca gaaaatattt gcaacacccc      780

agtaaagaga attgtaatat gcagattata aaaagaagtc ttacaaatca gtaaaaaata      840

tttctagaca aaaatttgaa cagatgaaag agaaactcta aataatcatt acacatgaga      900

aactcaatct cagaaatcag agaactatca ttgcatatac actaaattag agaaatatta      960

aaaggctaag taacatctgt ggcaatattg atggtatata accttgatat gatgtgatga     1020

gaacagtact ttaccccatg ggcttcctcc ccaaaccctt accccagtat aaatcatgac     1080

aaatatactt taaaaaccat taccctatat ctaaccagta ctcctcaaaa ctgtcaaggt     1140

catcaaaaat aagaaaagtc tgaggaactg tcaaaactaa gaggaaccca aggagacatg     1200

agaattatat gtaatgtggc attctgaatg agatcccaga acagaaaaag aacagtagct     1260

aaaaaactaa tgaaatataa atattttttg aactttagtt ttttttaaaa aagagtagca     1320

ttaacacggc aaagccattt tcatattttt cttgaacatt aagtacaagt ctataattaa     1380

aaatttttta aatgtagtct ggaacattgc cagaaacaga agtacagcag ctatctgtgc     1440

tgtcgcctaa ctatccatag ctgattggtc taaaatgaga tacatcaacg ctcctccatg     1500

ttttttgttt tctttttaaa tgaaaaactt tattttttaa gaggagtttc aggttcatag     1560

caaaattgag aggaaggtac attcaagctg aggaagtttt cctctattcc tagtttactg     1620

agagattgca tcatgaatgg gtgttaaatt ttgtcaaatg ctttttctgt gtctatcaat     1680

atgaccatgt gattttcttc tttaacctgt tgatgggaca aattacgtta attgattttc     1740

aaacgttgaa ccacccttac atatctggaa tattttctac ttggttgtgg tgtatatttt     1800

ttgatacatt cttggattct ttttgctaat attttgttga aaatgtttgt atctttgttc     1860

atgagagata ttggtctgtt gttttctttt cttgtaatgt cattttctag ttccggtatt     1920

aaggtaatgc tggcctagtt gaatgattta ggaagtattc cctctgcttc tgtcttctga     1980

aagagattgt agaaagttga tacaattttt ttttctttaa atatttgata gaattcttac     2040

tgacatccac tttgcctttc tctccacagg tgtccactcg gatcc                     2085


<210>  21
<211>  868
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splice donor-human Interferon beta M18 S/MAR-splice acceptor 
       flanked by 5' BglII site and 3' BamHI restriction enzyme sites

<400>  21
agatctatgc atgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa       60

gacaggtctc gagaagcttg atctaaataa acttataaat tgtgagagaa attaatgaat      120

gtctaagtta atgcagaaac ggagagacat actatattca tgaactaaaa gacttaatat      180

tgtgaaggta tactttcttt ccacataaat ttgtagtcaa tatgttcacc ccaaaaaagc      240

tgtttgttaa cttgccaacc tcattctaaa atgtatatag aagcccaaaa gacaataaca      300

aaaatattct tgtagaacaa aatgggaaag aatgttccac taaatatcaa gatttagagc      360

aaagcatgag atgtgtgggg atagacagtg aggctgataa aatagagtag agctcagaaa      420

cagacccatt gatatatgta agtgacctat gaaaaaaata tggcatttta caatgggaaa      480

atgatgatct ttttcttttt tagaaaaaca gggaaatata tttatatgta aaaaataaaa      540

gggaacccat atgtcatacc atacacacaa aaaaattcca gtgaattata agtctaaatg      600

gagaaggcaa aactttaaat cttttagaaa ataatataga agcatgccat catgacttca      660

gtgtagagaa aaatttctta tgactcaaag tcctaaccac aaagaaaaga ttgttaatta      720

tgaatgattt aggaagtatt ccctctgctt ctgtcttctg aaagagattg tagaaagttg      780

atacaatttt tttttcttta aatatttgat agagaattct tactgacatc cactttgcct      840

ttctctccac aggtgtccac tcggatcc                                         868


<210>  22
<211>  927
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splice donor-805 bp human Apolipoprotein B S/MAR-splice acceptor 
       flanked by 5' BglII site and 3' BamHI restriction enzyme sites

<400>  22
agatctatgc atgcagaagt tggtcgtgag gcactgggca ggtaagtatc aaggttacaa       60

gacaggtctc gaggcctacc ggagagcctt gccttgcaaa ggcagacagt cagtgaggaa      120

gactatgtgg cacatgaaga caccagaggt gttcctcagg atcaaagtat gtacaagcct      180

ttgtgaatat tttttccttc tcacttggca aatacaattc ctgagatcaa taacctcgtc      240

tttttaattt tttcctcgtc tttttaacta tttataaaat attgaattat aaaatatgta      300

attataaata ctttaattat aaaatatgta attataaata ctttaattat aaaatatgta      360

attataaata ctttataaaa tatgtaatta taaaatatgt aattataaac attttaatta      420

taaaatatgt aattataaac attttaatta taaaatatgt aattataaac attttaatta      480

taaaatatgt aattataaac attttaatta taaaatatgt aattataaac attttaatta      540

taaaatattt aattataaac attttaatta taaaatattt aattataaat attttaatta      600

taaaatattt aattataaat attttaatta taaaatattt aattataaat attttaatta      660

taaaatattt aattataaat actttaatta taaaatattt aattataaat attttaatta      720

taaaatattt aattataaat attttaatta taaatatttt aattataaaa tatttaatta      780

taaaaacaca attacctcat ctttttaaat atttttgcaa aatatttccc tccataattt      840

ctccgtttcc atttttattc tgttacttaa atgaattctt actgacatcc actttgcctt      900

tctctccaca ggtgtccact cggatcc                                          927


<210>  23
<211>  647
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splice donor-525 bp human Apolipoprotein B S/MAR-splice acceptor 
       flanked by 5' NsiI site and 3' BamHI restriction enzyme sites

<400>  23
atgcatgcag aagttggtcg tgaggcactg ggcaggtaag tatcaaggtt acaagacagg       60

tctcgagatt tataaaatat tgaattataa aatatgtaat tataaatact ttaattataa      120

aatatgtaat tataaatact ttaattataa aatatgtaat tataaatact ttataaaata      180

tgtaattata aaatatgtaa ttataaacat tttaattata aaatatgtaa ttataaacat      240

tttaattata aaatatgtaa ttataaacat tttaattata aaatatgtaa ttataaacat      300

tttaattata aaatatgtaa ttataaacat tttaattata aaatatttaa ttataaacat      360

tttaattata aaatatttaa ttataaatat tttaattata aaatatttaa ttataaatat      420

tttaattata aaatatttaa ttataaatat tttaattata aaatatttaa ttataaatac      480

tttaattata aaatatttaa ttataaatat tttaattata aaatatttaa ttataaatat      540

tttaattata aatattttaa ttataaaata tttaattata aaaacacaat tagaattctt      600

actgacatcc actttgcctt tctctccaca ggtgtccact cggatcc                    647


<210>  24
<211>  197
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCI intron

<400>  24
gcagaagttg gtcgtgaggc actgggcagg taagtatcaa ggttacaaga caggtttaag       60

gagaccaata gaaactgggc ttgtcgagac agagaagact cttgcgtttc tgataggcac      120

ctattggtct tactgacatc cactttgcct ttctctccac aggtgtccac tcccagttca      180

attacagctc ttaaggc                                                     197


<210>  25
<211>  42
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCI Splice donor

<400>  25
gcagaagttg gtcgtgaggc actgggcagg taagtatcaa gg                          42


<210>  26
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pCI Splice acceptor (murine IgG)

<400>  26
cttactgaca tccactttgc ctttctctcc acaggtgtcc actc                        44


<210>  27
<211>  1025
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Ele40 expression enhancer

<400>  27
gatcaagaaa gcactccggg ctccagaagg agccttccag gccagctttg agcataagct       60

gctgatgagc agtgagtgtc ttgagtagtg ttcagggcag catgttacca ttcatgcttg      120

acttctagcc agtgtgatga gaggctggag tcaggtctct agagagttga gcagctccag      180

ccttagatct cccagtctta tgcggtgtgc ccattcgctt tgtgtctgca gtcccctggc      240

cacacccagt aacagttctg ggatctatgg gagtagcttc cttagtgagc tttcccttca      300

aatactttgc aaccaggtag agaagtttgg agtgaaggtt ttgttctttg tttcttcaca      360

atatggatat gcatcttctt ttgaaaatgt taaagtaaat tacctctctt ttcagatact      420

gtcttcatgc gaacttggta tcctgtttcc atcccagcct tctataaccc agtaacatct      480

tttttgaaac cagtgggtga gaaagacacc tggtcaggaa cgcggaccac aggacaactc      540

aggctcaccc acggcatcag actaaaggca aacaaggact ctgtataaag taccggtggc      600

atgtgtatta gtggagatgc agcctgtgct ctgcagacag ggagtcacac agacactttt      660

ctataatttc ttaagtgctt tgaatgttca agtagaaagt ctaacattaa atttgattga      720

acaattgtat attcatggaa tattttggaa cggaatacca aaaaatggca atagtggttc      780

tttctggatg gaagacaaac ttttcttttt aaattttatc ttatatattt gaggttgacc      840

acatgacctt aaggatacat atagacagta aactggttac tacagtgaag caaattaaca      900

tatctaccat cttacatagt tacatttttt tgtgtgacag gaacagctaa aatctacgta      960

tttaacaaaa atcctaaaga caatacattt ttattaacta tagccctcat gatgtacatt     1020

agatc                                                                 1025


<210>  28
<211>  1503
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  A2UCOE expression enhancer

<400>  28
gcctacagct caagccacat ccgaaggggg agggagccgg gagctgcgcg cggggccgcc       60

ggggggaggg gtggcaccgc ccacgccggg cggccacgaa gggcggggca gcgggcgcgc      120

gcgcggcggg gggaggggcc ggcgccgcgc ccgctgggaa ttggggccct agggggaggg      180

cggaggcgcc gacgaccgcg gcacttaccg ttcgcggcgt ggcgcccggt ggtccccaag      240

gggagggaag ggggaggcgg ggcgaggaca gtgaccggag tctcctcagc ggtggctttt      300

ctgcttggca gcctcagcgg ctggcgccaa aaccggactc cgcccacttc ctcgcccgcc      360

ggtgcgaggg tgtggaatcc tccagacgct gggggagggg gagttgggag cttaaaaact      420

agtacccctt tgggaccact ttcagcagcg aactctcctg tacaccaggg gtcagttcca      480

cagacgcggg ccaggggtgg gtcattgcgg cgtgaacaat aatttgacta gaagttgatt      540

cgggtgtttc cggaaggggc cgagtcaatc cgccgagttg gggcacggaa aacaaaaagg      600

gaaggctact aagatttttc tggcgggggt tatcattggc gtaactgcag ggaccacctc      660

ccgggttgag ggggctggat ctccaggctg cggattaagc ccctcccgtc ggcgttaatt      720

tcaaactgcg cgaccgtttc tcacctgcct tgcgccaagg cagggggcgg gaccctattc      780

caagaggtag taactagcag gactctagcc ttccgcaatt cattgagcgc atttacggaa      840

gtaacgtcgg gtactgtctc tggccgcaag ggtgggagga gtacgcattt ggcgtaaggt      900

ggggcgtaga gccttcccgc cattggcggc ggatagggcg tttacgcgac ggcctgacgt      960

agcggaagac gcgttagtgg gggggaaggt tctagaaaag cggcggcagc ggctctagcg     1020

gcagtagcag cagcgccggg tcccgtgcgg aggtgctcct cgcagagttg tttctcgagc     1080

agcggcagtt ctcactacag cgccaggacg agtccggttc gtgttcgtcc gcggagatct     1140

ctctcatctc gctcggctgc gggaaatcgg gctgaagcga ctgagtccgc gatggaggta     1200

acgggtttga aatcaatgag ttattgaaaa gggcatggcg aggccgttgg cgcctcagtg     1260

gaagtcggcc agccgcctcc gtgggagaga ggcaggaaat cggaccaatt cagtagcagt     1320

ggggcttaag gtttatgaac ggggtcttga gcggaggcct gagcgtacaa acagcttccc     1380

caccctcagc ctcccggcgc catttccctt cactgggggt gggggatggg gagctttcac     1440

atggcggacg ctgccccgct ggggtgaaag tggggcgcgg aggcgggaat tcttattccc     1500

ttt                                                                   1503


<210>  29
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Splice acceptor consensus sequence

<400>  29
yyyyyyyyyy yagrw                                                        15


