                         SEQUENCE LISTING

<110>  baseclick GmbH
 
<120>  Click based ligation

<130>  64516P WO

<150>  EP 17 194 093
<151>  2017-09-29

<150>  EP 18 164 071
<151>  2018-03-26

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alkyne oligo1


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5'-alkyne dT

<400>  1
taatgatacg gcgaccaccg agatctacac tctttcccta cacgacgctc ttccgatct        59


<210>  2
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Azide oligo1


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5'-azide dT

<400>  2
tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagc        59


<210>  3
<211>  58
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  model RNA

<400>  3
uucgacaaac gaaaacacaa acacaaacca aacagaaaac aguacaugua aucgacca         58


<210>  4
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse transcription Primer1


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5'-FAM-dT

(FAM=Fluorescein)

<400>  4
tggtcgatta catgtac                                                      17


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse trascription Primer2

<400>  5
tggtcgatta catgtactgt ttt                                               23


<210>  6
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  reverse transcription Primer3

<400>  6
agatcggaag agcgtcg                                                      17


<210>  7
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA after reverse transcription


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5'-FAM-dT

(FAM=Fluorescein)

<220>
<221>  modified_base
<222>  (57)..(57)
<223>  3'-azide dA

<400>  7
tggtcgatta catgtactgt tttctgtttg gtttgtgttt gtgttttcgt ttgtcga          57


<210>  8
<211>  116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  click product


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5'-FAM dT

(FAM=Fluorescein)

<220>
<221>  misc_feature
<222>  (62)..(63)
<223>  A and T joined via backbone mimic B

<400>  8
tggtcgatta catgtactgt tttctgtttg gtttgtgttt gtgttttcgt ttgtcgataa       60

tgatacggcg accaccgaga tctacactct ttccctacac gacgctcttc cgatct          116


<210>  9
<211>  116
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  PCR product

<400>  9
tggtcgatta catgtactgt tttctgtttg gtttgtgttt gtgttttcgt ttgtcgataa       60

tgatacggcg accaccgaga tctacactct ttccctacac gacgctcttc cgatct          116


<210>  10
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fig.6 expected sequence

<400>  10
gttttctgtt tggtttgtgt ttgtgttttc gtttgtcgat aatgatacgg c                51


<210>  11
<211>  51
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fig.6 found sequence

<400>  11
gttttctgtt tggtttgtgt ttgtgttttc gtttgtcgat aatgatacgg c                51


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Fig.6 forward seq avg. QV=43

<400>  12
gtttgtcgat aatgatacgg c                                                 21


<210>  13
<211>  40
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illumina N6 primer


<220>
<221>  misc_feature
<222>  (35)..(40)
<223>  n is a, c, g, or t

<400>  13
gtgactggag ttcagacgtg tgctcttccg atctnnnnnn                             40


<210>  14
<211>  59
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alkyne adapter oligo


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5'-alkyne dT

<400>  14
taatgatacg gcgaccaccg agatctacac tctttcccta cacgacgctc ttccgatct        59


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P17-006 forw2 primer

<400>  15
gtgactggag ttcagacgtg                                                   20


<210>  16
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  P17-006 rev3 primer

<400>  16
gtcgtgtagg gaaagagtgt a                                                 21


<210>  17
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alkyne oligo biotin


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5-Biotin-TEG-dG

<220>
<221>  modified_base
<222>  (26)..(26)
<223>  C8-alkyne dU

<400>  17
gttctagaag gctaagaaaa atctcuacca                                        30


<210>  18
<211>  30
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Azide oligo biotin


<220>
<221>  modified_base
<222>  (1)..(1)
<223>  5-Biotin-TEG-dG

<220>
<221>  modified_base
<222>  (26)..(26)
<223>  PEG12 azide amino dT

<400>  18
gttctagaag gctaagaaaa atctctacca                                        30


