                         SEQUENCE LISTING

<110>  Oxford University Innovation Limited
 
<120>  PROTEASE INHIBITORS

<130>  P248023WO

<150>  GB 1715589.6
<151>  2017-09-26

<160>  46    

<170>  PatentIn version 3.5

<210>  1
<211>  226
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HSTIMP2 AA + NTPR1 SIGNAL PEPTIDE AA

<400>  1

Met Gly Phe Val Leu Phe Ser Gln Leu Pro Ser Phe Leu Leu Val Ser 
1               5                   10                  15      


Thr Leu Leu Leu Phe Leu Val Ile Ser His Ser Cys Arg Ala Gly Gly 
            20                  25                  30          


Cys Ser Cys Ser Pro Val His Pro Gln Gln Ala Phe Cys Asn Ala Asp 
        35                  40                  45              


Val Val Ile Arg Ala Lys Ala Val Ser Glu Lys Glu Val Asp Ser Gly 
    50                  55                  60                  


Asn Asp Ile Tyr Gly Asn Pro Ile Lys Arg Ile Gln Tyr Glu Ile Lys 
65                  70                  75                  80  


Gln Ile Lys Met Phe Lys Gly Pro Glu Lys Asp Ile Glu Phe Ile Tyr 
                85                  90                  95      


Thr Ala Pro Ser Ser Ala Val Cys Gly Val Ser Leu Asp Val Gly Gly 
            100                 105                 110         


Lys Lys Glu Tyr Leu Ile Ala Gly Lys Ala Glu Gly Asp Gly Lys Met 
        115                 120                 125             


His Ile Thr Leu Cys Asp Phe Ile Val Pro Trp Asp Thr Leu Ser Thr 
    130                 135                 140                 


Thr Gln Lys Lys Ser Leu Asn His Arg Tyr Gln Met Gly Cys Glu Cys 
145                 150                 155                 160 


Lys Ile Thr Arg Cys Pro Met Ile Pro Cys Tyr Ile Ser Ser Pro Asp 
                165                 170                 175     


Glu Cys Leu Trp Met Asp Trp Val Thr Glu Lys Asn Ile Asn Gly His 
            180                 185                 190         


Gln Ala Lys Phe Phe Ala Cys Ile Lys Arg Ser Asp Gly Ser Cys Ala 
        195                 200                 205             


Trp Tyr Arg Gly Ala Ala Pro Pro Lys Gln Glu Phe Leu Asp Ile Glu 
    210                 215                 220                 


Asp Pro 
225     


<210>  2
<211>  194
<212>  PRT
<213>  Homo sapiens

<400>  2

Cys Ser Cys Ser Pro Val His Pro Gln Gln Ala Phe Cys Asn Ala Asp 
1               5                   10                  15      


Val Val Ile Arg Ala Lys Ala Val Ser Glu Lys Glu Val Asp Ser Gly 
            20                  25                  30          


Asn Asp Ile Tyr Gly Asn Pro Ile Lys Arg Ile Gln Tyr Glu Ile Lys 
        35                  40                  45              


Gln Ile Lys Met Phe Lys Gly Pro Glu Lys Asp Ile Glu Phe Ile Tyr 
    50                  55                  60                  


Thr Ala Pro Ser Ser Ala Val Cys Gly Val Ser Leu Asp Val Gly Gly 
65                  70                  75                  80  


Lys Lys Glu Tyr Leu Ile Ala Gly Lys Ala Glu Gly Asp Gly Lys Met 
                85                  90                  95      


His Ile Thr Leu Cys Asp Phe Ile Val Pro Trp Asp Thr Leu Ser Thr 
            100                 105                 110         


Thr Gln Lys Lys Ser Leu Asn His Arg Tyr Gln Met Gly Cys Glu Cys 
        115                 120                 125             


Lys Ile Thr Arg Cys Pro Met Ile Pro Cys Tyr Ile Ser Ser Pro Asp 
    130                 135                 140                 


Glu Cys Leu Trp Met Asp Trp Val Thr Glu Lys Asn Ile Asn Gly His 
145                 150                 155                 160 


Gln Ala Lys Phe Phe Ala Cys Ile Lys Arg Ser Asp Gly Ser Cys Ala 
                165                 170                 175     


Trp Tyr Arg Gly Ala Ala Pro Pro Lys Gln Glu Phe Leu Asp Ile Glu 
            180                 185                 190         


Asp Pro 
        


<210>  3
<211>  5
<212>  PRT
<213>  Homo sapiens

<400>  3

Cys Ser Cys Ser Pro 
1               5   


<210>  4
<211>  7
<212>  PRT
<213>  Homo sapiens

<400>  4

Ala Pro Ser Ser Ala Val Cys 
1               5           


<210>  5
<211>  9
<212>  PRT
<213>  Homo sapiens

<400>  5

Ser Cys Ala Trp Tyr Arg Gly Ala Ala 
1               5                   


<210>  6
<211>  48
<212>  PRT
<213>  Homo sapiens

<400>  6

Pro Cys Tyr Ile Ser Ser Pro Asp Glu Cys Leu Trp Met Asp Trp Val 
1               5                   10                  15      


Thr Glu Lys Asn Ile Asn Gly His Gln Ala Lys Phe Phe Ala Cys Ile 
            20                  25                  30          


Lys Arg Ser Asp Gly Ser Cys Ala Trp Tyr Arg Gly Ala Ala Pro Pro 
        35                  40                  45              


<210>  7
<211>  58
<212>  PRT
<213>  Homo sapiens

<400>  7

Pro Cys Tyr Ile Ser Ser Pro Asp Glu Cys Leu Trp Met Asp Trp Val 
1               5                   10                  15      


Thr Glu Lys Asn Ile Asn Gly His Gln Ala Lys Phe Phe Ala Cys Ile 
            20                  25                  30          


Lys Arg Ser Asp Gly Ser Cys Ala Trp Tyr Arg Gly Ala Ala Pro Pro 
        35                  40                  45              


Lys Gln Glu Phe Leu Asp Ile Glu Asp Pro 
    50                  55              


<210>  8
<211>  28
<212>  PRT
<213>  Homo sapiens

<400>  8

Cys Ser Cys Ser Pro Val His Pro Gln Gln Ala Phe Cys Asn Ala Asp 
1               5                   10                  15      


Val Val Ile Arg Ala Lys Ala Val Ser Glu Lys Glu 
            20                  25              


<210>  9
<211>  585
<212>  DNA
<213>  Homo sapiens

<400>  9
tgttcttgct ctcctgttca tcctcagcag gctttctgca atgctgatgt ggtgattagg       60

gctaaggctg tgagcgagaa agaagtggat agcggtaacg atatctacgg taaccctatc      120

aagaggatcc agtacgagat caagcagatc aagatgttca agggtcctga gaaggatatc      180

gagtttatct acaccgctcc tagctctgct gtttgcggtg tttctcttga tgtgggtggt      240

aagaaagagt acctgatcgc tggtaaggct gagggtgatg gtaagatgca cattaccctg      300

tgcgatttca tcgtgccttg ggataccctt tcaaccactc agaagaagtc cctgaaccac      360

aggtatcaga tgggttgcga gtgcaagatt accaggtgcc ctatgatccc ttgctacatc      420

tcttcacctg atgagtgcct gtggatggat tgggttaccg agaagaacat caacggtcac      480

caggctaagt tcttcgcttg catcaagagg tccgatggtt cttgcgcttg gtatagaggt      540

gctgctcctc ctaagcaaga gttccttgat atcgaggatc cttag                      585


<210>  10
<211>  663
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HsTIMP2 DNA CODING SEQUENCE + NTPR1 SIGNAL PEPTIDE

<400>  10
atgggtgctg ctgctaggac tcttaggctt gctcttggtc tgcttcttct ggctactctt       60

cttaggcctg ctgatgcttg ttcttgctct cctgttcatc ctcagcaggc tttctgcaat      120

gctgatgtgg tgattagggc taaggctgtg agcgagaaag aagtggatag cggtaacgat      180

atctacggta accctatcaa gaggatccag tacgagatca agcagatcaa gatgttcaag      240

ggtcctgaga aggatatcga gtttatctac accgctccta gctctgctgt ttgcggtgtt      300

tctcttgatg tgggtggtaa gaaagagtac ctgatcgctg gtaaggctga gggtgatggt      360

aagatgcaca ttaccctgtg cgatttcatc gtgccttggg ataccctttc aaccactcag      420

aagaagtccc tgaaccacag gtatcagatg ggttgcgagt gcaagattac caggtgccct      480

atgatccctt gctacatctc ttcacctgat gagtgcctgt ggatggattg ggttaccgag      540

aagaacatca acggtcacca ggctaagttc ttcgcttgca tcaagaggtc cgatggttct      600

tgcgcttggt atagaggtgc tgctcctcct aagcaagagt tccttgatat cgaggatcct      660

tag                                                                    663


<210>  11
<211>  8
<212>  PRT
<213>  Nicotiana benthamiana

<400>  11

Thr Val Arg Ile Val Asp Gln Cys 
1               5               


<210>  12
<211>  7
<212>  PRT
<213>  Nicotiana benthamiana

<400>  12

Asn Gly Gly Leu Asp Leu Asp 
1               5           


<210>  13
<211>  10
<212>  PRT
<213>  Nicotiana benthamiana

<400>  13

Cys Gly Arg Cys Leu Arg Val Thr Asn Thr 
1               5                   10  


<210>  14
<211>  24
<212>  PRT
<213>  Nicotiana benthamiana

<400>  14

Leu Asp Thr Asn Gly Val Gly Tyr Gln Gln Gly His Leu Ile Val Asn 
1               5                   10                  15      


Tyr Glu Phe Ile Asn Cys Asp Asp 
            20                  


<210>  15
<211>  25
<212>  PRT
<213>  Nicotiana benthamiana

<400>  15

Ala Gly Gly Gln Ser Ala Thr Asn Val Arg Ser Thr Tyr His Leu Tyr 
1               5                   10                  15      


Asn Pro Gln Asn Ile Asn Trp Asp Leu 
            20                  25  


<210>  16
<211>  122
<212>  PRT
<213>  Nicotiana benthamiana

<400>  16

Gln Ser Ala Thr Asn Val Arg Ser Thr Tyr His Leu Tyr Asn Pro Gln 
1               5                   10                  15      


Asn Ile Asn Trp Asp Leu Arg Ala Ala Ser Ala Phe Cys Thr Thr Trp 
            20                  25                  30          


Asp Ala Asp Lys Pro Leu Thr Trp Arg Gln Lys Tyr Gly Trp Thr Ala 
        35                  40                  45              


Phe Cys Asp Ala Ala Gly Pro Gln Gly Gln Asp Ser Cys Gly Arg Cys 
    50                  55                  60                  


Leu Arg Val Thr Asn Thr Gly Thr Gly Thr Gln Thr Thr Val Arg Ile 
65                  70                  75                  80  


Val Asp Gln Cys Arg Asn Gly Gly Leu Asp Leu Asp Val Asn Val Phe 
                85                  90                  95      


Asn Gln Leu Asp Thr Asn Gly Val Gly Tyr Gln Gln Gly His Leu Ile 
            100                 105                 110         


Val Asn Tyr Glu Phe Ile Asn Cys Asp Asp 
        115                 120         


<210>  17
<211>  657
<212>  DNA
<213>  Nicotiana benthamiana

<400>  17
cagagcgcta caaacgtgag atcaacgtat catttataca acccacagaa cattaactgg       60

gatttgagag cagcaagtgc tttctgcact acttgggatg ccgacaagcc tctcacgtgg      120

cgtcagaaat atggctggac tgctttctgt gatgctgctg gacctcaagg ccaagattcc      180

tgtggtagat gcttgagggt acgacatctt caattttttg ttactatatc aattttgtca      240

tgaaagattt acctattgat gtaggtcaac ttttacctaa tggtatatat gtcggtgcac      300

ataatttaaa ctctatagtt tgttttagag tttaacttct atatacttgt cggtataaaa      360

gcattttgca ctatcaaatg tttactataa gtaaattatt tgaaagagaa aatgccgtaa      420

gtagcaaagt aactaaaaat actgaatacc tggatatgta ttaatggtaa agctaaacgc      480

aggtgacgaa cacaggaaca ggaactcaaa caacagtgag aatagtagat caatgcagaa      540

atggagggct tgatttggat gtaaacgtct ttaaccaatt ggacacaaat ggagtgggct      600

atcagcaagg ccaccttatt gtcaactatg aatttatcaa ctgcgatgac taagctt         657


<210>  18
<211>  747
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NBPR4 DNA CODING SEQUENCE + INTRON + NTPR1 SIGNAL PEPTIDE

<400>  18
atgggatttg ttctcttttc acaattgcct tcatttcttc ttgtctctac acttctctta       60

ttcctagtaa tatcccactc ttgccgtgca cagagcgcta caaacgtgag atcaacgtat      120

catttataca acccacagaa cattaactgg gatttgagag cagcaagtgc tttctgcact      180

acttgggatg ccgacaagcc tctcacgtgg cgtcagaaat atggctggac tgctttctgt      240

gatgctgctg gacctcaagg ccaagattcc tgtggtagat gcttgagggt acgacatctt      300

caattttttg ttactatatc aattttgtca tgaaagattt acctattgat gtaggtcaac      360

ttttacctaa tggtatatat gtcggtgcac ataatttaaa ctctatagtt tgttttagag      420

tttaacttct atatacttgt cggtataaaa gcattttgca ctatcaaatg tttactataa      480

gtaaattatt tgaaagagaa aatgccgtaa gtagcaaagt aactaaaaat actgaatacc      540

tggatatgta ttaatggtaa agctaaacgc aggtgacgaa cacaggaaca ggaactcaaa      600

caacagtgag aatagtagat caatgcagaa atggagggct tgatttggat gtaaacgtct      660

ttaaccaatt ggacacaaat ggagtgggct atcagcaagg ccaccttatt gtcaactatg      720

aatttatcaa ctgcgatgac taagctt                                          747


<210>  19
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NbPot1 N-terminal motif


<220>
<221>  X
<222>  (2)..(2)
<223>  X is one of Histidine, Serine, Aspartic Acid, Threonine, Lysine, 
       Glutamine or Valine

<220>
<221>  X
<222>  (3)..(3)
<223>  X is one of Leucine, Serine, Lysine, Glutamic Acid, Glutamine, or
       Methionine

<400>  19

Lys Xaa Xaa Trp Pro Glu Leu Val Gly 
1               5                   


<210>  20
<211>  9
<212>  PRT
<213>  Nicotiana benthamiana

<400>  20

Lys His Leu Trp Pro Glu Leu Val Gly 
1               5                   


<210>  21
<211>  13
<212>  PRT
<213>  Nicotiana benthamiana

<400>  21

Asn Trp Glu Phe Ile Val Ile Ile Thr Pro Thr Val Gly 
1               5                   10              


<210>  22
<211>  18
<212>  PRT
<213>  Nicotiana benthamiana

<400>  22

Gly Met Pro Gly Lys Thr Ala Lys Glu Ile Ile Glu Lys Glu Asn Pro 
1               5                   10                  15      


Leu Val 
        


<210>  23
<211>  63
<212>  PRT
<213>  Nicotiana benthamiana

<400>  23

Lys His Leu Trp Pro Glu Leu Val Gly Met Pro Gly Lys Thr Ala Lys 
1               5                   10                  15      


Glu Ile Ile Glu Lys Glu Asn Pro Leu Val Lys Val Gln Phe Leu Phe 
            20                  25                  30          


Pro Gly Met Pro Lys Pro Leu Asp Leu Val Cys Gly Arg Val Ile Val 
        35                  40                  45              


Val Val Asn Trp Glu Phe Ile Val Ile Ile Thr Pro Thr Val Gly 
    50                  55                  60              


<210>  24
<211>  387
<212>  DNA
<213>  Nicotiana benthamiana

<400>  24
ggtaagtttc tgcttctacc tttgatatat atataataat tatcattaat tagtagtaat       60

ataatatttc aaatattttt ttcaaaataa aagaatgtag tatatagcaa ttgcttttct      120

gtagtttata agtgtgtata ttttaattta taacttttct aatatatgac caaaatttgt      180

tgatgtgcag gaggtaagca tttatggcct gaacttgtgg gaatgccagg aaagactgct      240

aaggaaataa ttgagaaaga aaatcccttg gtcaaagttc aatttttgtt ccctggaatg      300

cctaaaccat tggatttagt ttgtggtcga gttattgttg ttgttaactg ggaattcatc      360

gttataatta ctcccacagt gggttaa                                          387


<210>  25
<211>  477
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NBPOT1 NT + NTPR1 SIGNAL PEPTIDE NT

<400>  25
atgggatttg ttctcttttc acaattgcct tcatttcttc ttgtctctac acttctctta       60

ttcctagtaa tatcccactc ttgccgtgca ggtaagtttc tgcttctacc tttgatatat      120

atataataat tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa      180

aagaatgtag tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta      240

taacttttct aatatatgac caaaatttgt tgatgtgcag gaggtaagca tttatggcct      300

gaacttgtgg gaatgccagg aaagactgct aaggaaataa ttgagaaaga aaatcccttg      360

gtcaaagttc aatttttgtt ccctggaatg cctaaaccat tggatttagt ttgtggtcga      420

gttattgttg ttgttaactg ggaattcatc gttataatta ctcccacagt gggttaa         477


<210>  26
<211>  154
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NBPR4 AA + NTPR1 SIGNAL PEPTIDE AA

<400>  26

Met Gly Phe Val Leu Phe Ser Gln Leu Pro Ser Phe Leu Leu Val Ser 
1               5                   10                  15      


Thr Leu Leu Leu Phe Leu Val Ile Ser His Ser Cys Arg Ala Gly Gly 
            20                  25                  30          


Gln Ser Ala Thr Asn Val Arg Ser Thr Tyr His Leu Tyr Asn Pro Gln 
        35                  40                  45              


Asn Ile Asn Trp Asp Leu Arg Ala Ala Ser Ala Phe Cys Thr Thr Trp 
    50                  55                  60                  


Asp Ala Asp Lys Pro Leu Thr Trp Arg Gln Lys Tyr Gly Trp Thr Ala 
65                  70                  75                  80  


Phe Cys Asp Ala Ala Gly Pro Gln Gly Gln Asp Ser Cys Gly Arg Cys 
                85                  90                  95      


Leu Arg Val Thr Asn Thr Gly Thr Gly Thr Gln Thr Thr Val Arg Ile 
            100                 105                 110         


Val Asp Gln Cys Arg Asn Gly Gly Leu Asp Leu Asp Val Asn Val Phe 
        115                 120                 125             


Asn Gln Leu Asp Thr Asn Gly Val Gly Tyr Gln Gln Gly His Leu Ile 
    130                 135                 140                 


Val Asn Tyr Glu Phe Ile Asn Cys Asp Asp 
145                 150                 


<210>  27
<211>  95
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NBPOT1 AA + NTPR1 SIGNAL PEPTIDE AA

<400>  27

Met Gly Phe Val Leu Phe Ser Gln Leu Pro Ser Phe Leu Leu Val Ser 
1               5                   10                  15      


Thr Leu Leu Leu Phe Leu Val Ile Ser His Ser Cys Arg Ala Gly Gly 
            20                  25                  30          


Lys His Leu Trp Pro Glu Leu Val Gly Met Pro Gly Lys Thr Ala Lys 
        35                  40                  45              


Glu Ile Ile Glu Lys Glu Asn Pro Leu Val Lys Val Gln Phe Leu Phe 
    50                  55                  60                  


Pro Gly Met Pro Lys Pro Leu Asp Leu Val Cys Gly Arg Val Ile Val 
65                  70                  75                  80  


Val Val Asn Trp Glu Phe Ile Val Ile Ile Thr Pro Thr Val Gly 
                85                  90                  95  


<210>  28
<211>  32
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NTPR1 SIGNAL PEPTIDE AA + GLY-GLY LINKER

<400>  28

Met Gly Phe Val Leu Phe Ser Gln Leu Pro Ser Phe Leu Leu Val Ser 
1               5                   10                  15      


Thr Leu Leu Leu Phe Leu Val Ile Ser His Ser Cys Arg Ala Gly Gly 
            20                  25                  30          


<210>  29
<211>  285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NTPR1 SP DNA USED TO REPLACE NATIVE SIGNAL PEPTIDES (CDS, INTRON,
       GLY-GLY LINKER)

<400>  29
atgggatttg ttctcttttc acaattgcct tcatttcttc ttgtctctac acttctctta       60

ttcctagtaa tatcccactc ttgccgtgca ggtaagtttc tgcttctacc tttgatatat      120

atataataat tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa      180

aagaatgtag tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta      240

taacttttct aatatatgac caaaatttgt tgatgtgcag gaggt                      285


<210>  30
<211>  219
<212>  PRT
<213>  Homo sapiens

<400>  30

Gly Ala Ala Ala Arg Thr Leu Arg Leu Ala Leu Gly Leu Leu Leu Leu 
1               5                   10                  15      


Ala Thr Leu Leu Arg Pro Ala Asp Ala Cys Ser Cys Ser Pro Val His 
            20                  25                  30          


Pro Gln Gln Ala Phe Cys Asn Ala Asp Val Val Ile Arg Ala Lys Ala 
        35                  40                  45              


Val Ser Glu Lys Glu Val Asp Ser Gly Asn Asp Ile Tyr Gly Asn Pro 
    50                  55                  60                  


Ile Lys Arg Ile Gln Tyr Glu Ile Lys Gln Ile Lys Met Phe Lys Gly 
65                  70                  75                  80  


Pro Glu Lys Asp Ile Glu Phe Ile Tyr Thr Ala Pro Ser Ser Ala Val 
                85                  90                  95      


Cys Gly Val Ser Leu Asp Val Gly Gly Lys Lys Glu Tyr Leu Ile Ala 
            100                 105                 110         


Gly Lys Ala Glu Gly Asp Gly Lys Met His Ile Thr Leu Cys Asp Phe 
        115                 120                 125             


Ile Val Pro Trp Asp Thr Leu Ser Thr Thr Gln Lys Lys Ser Leu Asn 
    130                 135                 140                 


His Arg Tyr Gln Met Gly Cys Glu Cys Lys Ile Thr Arg Cys Pro Met 
145                 150                 155                 160 


Ile Pro Cys Tyr Ile Ser Ser Pro Asp Glu Cys Leu Trp Met Asp Trp 
                165                 170                 175     


Val Thr Glu Lys Asn Ile Asn Gly His Gln Ala Lys Phe Phe Ala Cys 
            180                 185                 190         


Ile Lys Arg Ser Asp Gly Ser Cys Ala Trp Tyr Arg Gly Ala Ala Pro 
        195                 200                 205             


Pro Lys Gln Glu Phe Leu Asp Ile Glu Asp Pro 
    210                 215                 


<210>  31
<211>  1712
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HSTIMP2 EXPRESSION CONSTRUCT (35S: NTPR1SP:HSTIMP2)

<400>  31
gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca       60

gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga      120

ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc      300

acgtctacaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctggtctac      360

tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa      420

aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa      480

aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct      540

atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggaca cgctcgagta taagagctca tttttacaac      780

aattaccaac aacaacaaac aacaaacaac attacaatta catttacaat tatcgatacg      840

aatgggtgct gctgctagga ctcttaggct tgctcttggt ctgcttcttc tggctactct      900

tcttaggcct gctgatgctt gttcttgctc tcctgttcat cctcagcagg ctttctgcaa      960

tgctgatgtg gtgattaggg ctaaggctgt gagcgagaaa gaagtggata gcggtaacga     1020

tatctacggt aaccctatca agaggatcca gtacgagatc aagcagatca agatgttcaa     1080

gggtcctgag aaggatatcg agtttatcta caccgctcct agctctgctg tttgcggtgt     1140

ttctcttgat gtgggtggta agaaagagta cctgatcgct ggtaaggctg agggtgatgg     1200

taagatgcac attaccctgt gcgatttcat cgtgccttgg gatacccttt caaccactca     1260

gaagaagtcc ctgaaccaca ggtatcagat gggttgcgag tgcaagatta ccaggtgccc     1320

tatgatccct tgctacatct cttcacctga tgagtgcctg tggatggatt gggttaccga     1380

gaagaacatc aacggtcacc aggctaagtt cttcgcttgc atcaagaggt ccgatggttc     1440

ttgcgcttgg tatagaggtg ctgctcctcc taagcaagag ttccttgata tcgaggatcc     1500

ttaggcttct ctagctagag tcgatcgaca agctcgagtt tctccataat aatgtgtgag     1560

tagttcccag ataagggaat tagggttcct atagggtttc gctcatgtgt tgagcatata     1620

agaaaccctt agtatgtatt tgtatttgta aaatacttct atcaataaaa tttctaattc     1680

ctaaaaccaa aatccagtac taaaatccag at                                   1712


<210>  32
<211>  148
<212>  PRT
<213>  Nicotiana benthamiana

<400>  32

Met Glu Arg Val Asn Asn Tyr Tyr Lys Leu Cys Met Glu Leu Phe Ile 
1               5                   10                  15      


Met Ser Met Met Val Ala Met Ala Ala Ala Gln Ser Ala Thr Asn Val 
            20                  25                  30          


Arg Ser Thr Tyr His Leu Tyr Asn Pro Gln Asn Ile Asn Trp Asp Leu 
        35                  40                  45              


Arg Ala Ala Ser Ala Phe Cys Thr Thr Trp Asp Ala Asp Lys Pro Leu 
    50                  55                  60                  


Thr Trp Arg Gln Lys Tyr Gly Trp Thr Ala Phe Cys Asp Ala Ala Gly 
65                  70                  75                  80  


Pro Gln Gly Gln Asp Ser Cys Gly Arg Cys Leu Arg Val Thr Asn Thr 
                85                  90                  95      


Gly Thr Gly Thr Gln Thr Thr Val Arg Ile Val Asp Gln Cys Arg Asn 
            100                 105                 110         


Gly Gly Leu Asp Leu Asp Val Asn Val Phe Asn Gln Leu Asp Thr Asn 
        115                 120                 125             


Gly Val Gly Tyr Gln Gln Gly His Leu Ile Val Asn Tyr Glu Phe Ile 
    130                 135                 140                 


Asn Cys Asp Asp 
145             


<210>  33
<211>  1792
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NBPR4 EXPRESSION CONSTRUCT (35S PROMOTER AND TERMINATOR + NTPR1 
       SIGNAL PEPTIDE + NBPR4 CODING SEQUENCE)

<400>  33
gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca       60

gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga      120

ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc      300

acgtctacaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctggtctac      360

tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa      420

aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa      480

aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct      540

atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggaca cgctcgagta taagagctca tttttacaac      780

aattaccaac aacaacaaac aacaaacaac attacaatta catttacaat tatcgatacg      840

aatgggattt gttctctttt cacaattgcc ttcatttctt cttgtctcta cacttctctt      900

attcctagta atatcccact cttgccgtgc acagagcgct acaaacgtga gatcaacgta      960

tcatttatac aacccacaga acattaactg ggatttgaga gcagcaagtg ctttctgcac     1020

tacttgggat gccgacaagc ctctcacgtg gcgtcagaaa tatggctgga ctgctttctg     1080

tgatgctgct ggacctcaag gccaagattc ctgtggtaga tgcttgaggg tacgacatct     1140

tcaatttttt gttactatat caattttgtc atgaaagatt tacctattga tgtaggtcaa     1200

cttttaccta atggtatata tgtcggtgca cataatttaa actctatagt ttgttttaga     1260

gtttaacttc tatatacttg tcggtataaa agcattttgc actatcaaat gtttactata     1320

agtaaattat ttgaaagaga aaatgccgta agtagcaaag taactaaaaa tactgaatac     1380

ctggatatgt attaatggta aagctaaacg caggtgacga acacaggaac aggaactcaa     1440

acaacagtga gaatagtaga tcaatgcaga aatggagggc ttgatttgga tgtaaacgtc     1500

tttaaccaat tggacacaaa tggagtgggc tatcagcaag gccaccttat tgtcaactat     1560

gaatttatca actgcgatga ctaagcttct ctagctagag tcgatcgaca agctcgagtt     1620

tctccataat aatgtgtgag tagttcccag ataagggaat tagggttcct atagggtttc     1680

gctcatgtgt tgagcatata agaaaccctt agtatgtatt tgtatttgta aaatacttct     1740

atcaataaaa tttctaattc ctaaaaccaa aatccagtac taaaatccag at             1792


<210>  34
<211>  192
<212>  DNA
<213>  Nicotiana benthamiana

<400>  34
aagcatttat ggcctgaact tgtgggaatg ccaggaaaga ctgctaagga aataattgag       60

aaagaaaatc ccttggtcaa agttcaattt ttgttccctg gaatgcctaa accattggat      120

ttagtttgtg gtcgagttat tgttgttgtt aactgggaat tcatcgttat aattactccc      180

acagtgggtt aa                                                          192


<210>  35
<211>  1525
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NBPOT1 EXPRESSION CONSTRUCT (35S PROMOTER AND TERMINATOR, NTPR1 
       SIGNAL PEPTIDE, CODING SEQUENCE)

<400>  35
gtcaacatgg tggagcacga cactctggtc tactccaaaa atgtcaaaga tacagtctca       60

gaagatcaaa gggctattga gacttttcaa caaaggataa tttcgggaaa cctcctcgga      120

ttccattgcc cagctatctg tcacttcatc gaaaggacag tagaaaagga aggtggctcc      180

tacaaatgcc atcattgcga taaaggaaag gctatcattc aagatctctc tgccgacagt      240

ggtcccaaag atggaccccc acccacgagg agcatcgtgg aaaaagaaga ggttccaacc      300

acgtctacaa agcaagtgga ttgatgtgat aacatggtgg agcacgacac tctggtctac      360

tccaaaaatg tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa      420

aggataattt cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa      480

aggacagtag aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct      540

atcattcaag atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc      600

atcgtggaaa aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgacatc      660

tccactgacg taagggatga cgcacaatcc cactatcctt cgcaagaccc ttcctctata      720

taaggaagtt catttcattt ggagaggaca cgctcgagta taagagctca tttttacaac      780

aattaccaac aacaacaaac aacaaacaac attacaatta catttacaat tatcgataca      840

atgggatttg ttctcttttc acaattgcct tcatttcttc ttgtctctac acttctctta      900

ttcctagtaa tatcccactc ttgccgtgca ggtaagtttc tgcttctacc tttgatatat      960

atataataat tatcattaat tagtagtaat ataatatttc aaatattttt ttcaaaataa     1020

aagaatgtag tatatagcaa ttgcttttct gtagtttata agtgtgtata ttttaattta     1080

taacttttct aatatatgac caaaatttgt tgatgtgcag gaggtaagca tttatggcct     1140

gaacttgtgg gaatgccagg aaagactgct aaggaaataa ttgagaaaga aaatcccttg     1200

gtcaaagttc aatttttgtt ccctggaatg cctaaaccat tggatttagt ttgtggtcga     1260

gttattgttg ttgttaactg ggaattcatc gttataatta ctcccacagt gggttaagct     1320

tctctagcta gagtcgatcg acaagctcga gtttctccat aataatgtgt gagtagttcc     1380

cagataaggg aattagggtt cctatagggt ttcgctcatg tgttgagcat ataagaaacc     1440

cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa ttcctaaaac     1500

caaaatccag tactaaaatc cagat                                           1525


<210>  36
<211>  46
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer #001 used for NbPR4 amplification (NbPR4_natSP_FW)

<400>  36
gatcgatctt gaagacaaaa tggagagagt aaataattac tataag                      46


<210>  37
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer #003 used for NbRP4 amplification (NbPR4_REV)

<400>  37
ttgaagacaa aagcttagtc atcgcagttg ataaattcat ag                          42


<210>  38
<211>  20
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer #005 used for NbPR4 amplification (NbPR4_noSP_FW)

<400>  38
cagagcgcta caaacgtgag                                                   20


<210>  39
<211>  42
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer #074 used for NbPR4 amplification (NbPot1_REV)

<400>  39
ttgaagacaa aagcttaacc cactgtggga gtaattataa cg                          42


<210>  40
<211>  39
<212>  DNA
<213>  artificial sequence

<220>
<223>  Primer #075 used for NbPR4 amplification (NbPot1_FW)

<400>  40
ttgaagacaa aggtaagcat ttatggcctg aacttgtgg                              39


<210>  41
<211>  6
<212>  PRT
<213>  unknown

<220>
<223>  Sequence replacing the native N-terminus of NtPR1 (SEQ ID NO 3) 
       in a mutant version of NtPR1 that does not enhance RP 
       accumulation

<400>  41

Ala Cys Ser Cys Ser Pro 
1               5       


<210>  42
<211>  5
<212>  PRT
<213>  unknown

<220>
<223>  Sequence replacing the native N-terminus of NtPR1 (SEQ ID NO 3) 
       in a mutant version of NtPR1 that does not enhance RP 
       accumulation

<400>  42

Ala Ser Ala Ser Pro 
1               5   


<210>  43
<211>  18
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence for flag-HA tag

<400>  43

Gly Asp Tyr Lys Asp Asp Asp Asp Lys Tyr Pro Tyr Asp Val Pro Asp 
1               5                   10                  15      


Tyr Ala 
        


<210>  44
<211>  57
<212>  DNA
<213>  artificial sequence

<220>
<223>  coding sequence for flag-HA tag

<400>  44
ggtgactaca aggacgacga tgacaagtac ccatacgatg ttccagatta cgcttaa          57


<210>  45
<211>  8
<212>  PRT
<213>  artificial sequence

<220>
<223>  protein sequence for 6xHis tag

<400>  45

His His His His His His Gly Gly 
1               5               


<210>  46
<211>  24
<212>  DNA
<213>  artificial sequence

<220>
<223>  coding sequence for 6xHis tag

<400>  46
catcaccatc accatcacgg aggt                                              24


