                         SEQUENCE LISTING

<110>  LONGWOOD UNIVERSITY
 
<120>  PD1-SPECIFIC CHIMERIC ANTIGEN RECEPTOR AS AN IMMUNOTHERAPY

<130>  14860001TA

<160>  9     

<170>  PatentIn version 3.5

<210>  1
<211>  288
<212>  PRT
<213>  Homo sapiens

<400>  1

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly Val Val Gly Gly 
                165                 170                 175     


Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu Ala Val Ile Cys 
            180                 185                 190         


Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro 
        195                 200                 205             


Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly 
    210                 215                 220                 


Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro 
225                 230                 235                 240 


Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly 
                245                 250                 255     


Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg 
            260                 265                 270         


Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
        275                 280                 285             


<210>  2
<211>  1079
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic chimeric antigen receptor

<400>  2
agtttccctt ccgctcacct ccgcctgagc agtggagaag gcggcactct ggtggggctg       60

ctccaggcat gcagatccca caggcgccct ggccagtcgt ctgggcggtg ctacaactgg      120

gctggcggcc aggatggttc ttagactccc cagacaggcc ctggaacccc cccaccttct      180

ccccagccct gctcgtggtg accgaagggg acaacgccac cttcacctgc agcttctcca      240

acacatcgga gagcttcgtg ctaaactggt accgcatgag ccccagcaac cagacggaca      300

agctggccgc cttccccgag gaccgcagcc agcccggcca ggactgccgc ttccgtgtca      360

cacaactgcc caacgggcgt gacttccaca tgagcgtggt cagggcccgg cgcaatgaca      420

gcggcaccta cctctgtggg gccatctccc tggcccccaa ggcgcagatc aaagagagcc      480

tgcgggcaga gctcagggtg acagagagaa gggcagaagt gcccacagcc caccccagcc      540

cctcacccag gccagccggc cagttccaaa ccctggtgat ctacatctgg gcgcccttgg      600

ccgggacttg tggggtcctt ctcctgtcac tggttatcac cctgtgcgca cgcccacgcc      660

gcagccccgc ccaagaagat ggcaaagtct acatcaacat gccaggcagg ggcggtgtca      720

ttctcactgc cttgttcctg agagtgaagt tcagcaggag cgcagacgcc cccgcgtacc      780

agcagggcca gaaccagctc tataacgagc tcaatctagg acgaagagag gagtacgatg      840

ttttggacaa gagacgtggc cgggaccctg agatgggggg aaagccgaga aggaagaacc      900

ctcaggaagg cctgtacaat gaactgcaga aagataagat ggcggaggcc tacagtgaga      960

ttgggatgaa aggcgagcgc cggaggggca aggggcacga tggcctttac cagggtctca     1020

gtacagccac caaggacacc tacgacgccc ttcacatgca ggccctgccc cctcgctaa      1079


<210>  3
<211>  337
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic chimeric antigen receptor

<400>  3

Met Gln Ile Pro Gln Ala Pro Trp Pro Val Val Trp Ala Val Leu Gln 
1               5                   10                  15      


Leu Gly Trp Arg Pro Gly Trp Phe Leu Asp Ser Pro Asp Arg Pro Trp 
            20                  25                  30          


Asn Pro Pro Thr Phe Ser Pro Ala Leu Leu Val Val Thr Glu Gly Asp 
        35                  40                  45              


Asn Ala Thr Phe Thr Cys Ser Phe Ser Asn Thr Ser Glu Ser Phe Val 
    50                  55                  60                  


Leu Asn Trp Tyr Arg Met Ser Pro Ser Asn Gln Thr Asp Lys Leu Ala 
65                  70                  75                  80  


Ala Phe Pro Glu Asp Arg Ser Gln Pro Gly Gln Asp Cys Arg Phe Arg 
                85                  90                  95      


Val Thr Gln Leu Pro Asn Gly Arg Asp Phe His Met Ser Val Val Arg 
            100                 105                 110         


Ala Arg Arg Asn Asp Ser Gly Thr Tyr Leu Cys Gly Ala Ile Ser Leu 
        115                 120                 125             


Ala Pro Lys Ala Gln Ile Lys Glu Ser Leu Arg Ala Glu Leu Arg Val 
    130                 135                 140                 


Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro 
145                 150                 155                 160 


Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Ile Tyr Ile Trp Ala Pro 
                165                 170                 175     


Leu Ala Gly Thr Cys Gly Val Leu Leu Leu Ser Leu Val Ile Thr Leu 
            180                 185                 190         


Cys Ala Arg Pro Arg Arg Ser Pro Ala Gln Glu Asp Gly Lys Val Tyr 
        195                 200                 205             


Ile Asn Met Pro Gly Arg Gly Gly Val Ile Leu Thr Ala Leu Phe Leu 
    210                 215                 220                 


Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
225                 230                 235                 240 


Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
                245                 250                 255     


Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
            260                 265                 270         


Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
        275                 280                 285             


Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
    290                 295                 300                 


Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
305                 310                 315                 320 


Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
                325                 330                 335     


Arg 
    


<210>  4
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  4
gtgtgatggt gggaatgggt caga                                              24


<210>  5
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  5
tacgaccaga ggcatacagg gaca                                              24


<210>  6
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  6
gctccaaagg acttgtacgt g                                                 21


<210>  7
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  7
tgatctgaag ggcagcattt c                                                 21


<210>  8
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  8
ctgccgatac tgaacctgag c                                                 21


<210>  9
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic primer

<400>  9
gcggtcaaaa tcgcactc                                                     18


