                         SEQUENCE LISTING

<110>  ImmPACT-Bio Ltd.
       Gavish-Galilee Bio Applications, Ltd.
       Gross, Gideon
       Gibson, Will
       Dahary, Dvir
       Beiman, Merav
 
<120>  A UNIVERSAL PLATFORM FOR CAR THERAPY TARGETING A NOVEL ANTIGENIC 
       SIGNATURE OF CANCER

<130>  120575-5003-WO

<150>  62/564,454
<151>  2017-09-28

<150>  62/649,429
<151>  2018-03-28

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  3432
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD19 aCAR_IRES_RFP_P2A_Puro Synthetic DNA

<400>  1
atggccttac cagtgaccgc cttgctcctg ccgctggcct tgctgctcca cgccgccagg       60

ccggacatcc agatgacaca gactacatcc tccctgtctg cctctctggg agacagagtc      120

accatcagtt gcagggcaag tcaggacatt agtaaatatt taaattggta tcagcagaaa      180

ccagatggaa ctgttaaact cctgatctac catacatcaa gattacactc aggagtccca      240

tcaaggttca gtggcagtgg gtctggaaca gattattctc tcaccattag caacctggag      300

caagaagata ttgccactta cttttgccaa cagggtaata cgcttccgta cacgttcgga      360

ggggggacca agctggagat cacaggtggc ggtggctcgg gcggtggtgg gtcgggtggc      420

ggcggatctg aggtgaaact gcaggagtca ggacctggcc tggtggcgcc ctcacagagc      480

ctgtccgtca catgcactgt ctcaggggtc tcattacccg actatggtgt aagctggatt      540

cgccagcctc cacgaaaggg tctggagtgg ctgggagtaa tatggggtag tgaaaccaca      600

tactataatt cagctctcaa atccagactg accatcatca aggacaactc caagagccaa      660

gttttcttaa aaatgaacag tctgcaaact gatgacacag ccatttacta ctgtgccaaa      720

cattattact acggtggtag ctatgctatg gactactggg gccaaggaac ctcagtcacc      780

gtctcctcaa ccactacccc agcaccgagg ccacccaccc cggctcctac catcgcctcc      840

cagcctctgt ccctgcgtcc ggaggcatgt agacccgcag ctggtggggc cgtgcatacc      900

cggggtcttg acttcgcctg cgatatctac atttgggccc ctctggctgg tacttgcggg      960

gtcctgctgc tttcactcgt gatcactctt tactgtaagc gcggtcggaa gaagctgctg     1020

tacatcttta agcaaccctt catgaggcct gtgcagacta ctcaagagga ggacggctgt     1080

tcatgccggt tcccagagga ggaggaaggc ggctgcgaac tgcgcgtgaa attcagccgc     1140

agcgcagatg ctccagccta caagcagggg cagaaccagc tctacaacga actcaatctt     1200

ggtcggagag aggagtacga cgtgctggac aagcggagag gacgggaccc agaaatgggc     1260

gggaagccgc gcagaaagaa tccccaagag ggcctgtaca acgagctcca aaaggataag     1320

atggcagaag cctatagcga gattggtatg aaaggggaac gcagaagagg caaaggccac     1380

gacggactgt accagggact cagcaccgcc accaaggaca cctatgacgc tcttcacatg     1440

caggccctgc cgcctcggtg agcggccgca aattccgccc ctctccctcc ccccccccta     1500

acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt     1560

ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga     1620

cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg     1680

tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt     1740

gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat     1800

aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg     1860

aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg     1920

taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt     1980

cgaggttaaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa     2040

cacgataata ccatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag     2100

ctgtacatgg agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc     2160

aagccctacg agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc     2220

ttcgccttcg acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac     2280

acccagggca tccccgactt ctttaagcag tccttccctg agggcttcac atgggagaga     2340

gtcaccacat acgaagacgg gggcgtgctg accgctaccc aggacaccag cctccaggac     2400

ggctgcctca tctacaacgt caagatcaga ggggtgaact tcccatccaa cggccctgtg     2460

atgcagaaga aaacactcgg ctgggaggcc aacaccgaga tgctgtaccc cgctgacggc     2520

ggcctggaag gcagaagcga catggccctg aagctcgtgg gcgggggcca cctgatctgc     2580

aacttcaaga ccacatacag atccaagaaa cccgctaaga acctcaagat gcccggcgtc     2640

tactatgtgg accacagact ggaaagaatc aaggaggccg acaaagagac ctacgtcgag     2700

cagcacgagg tggctgtggc cagatactgc gacctcccta gcaaactggg gcacaaactt     2760

aatggatccg gcgcgacaaa ctttagcttg ctgaagcaag ctggtgacgt ggaggagaat     2820

cccggcccta tggccaccga gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc     2880

ccccgggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac gcgccacacc     2940

gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt cctcacgcgc     3000

gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg     3060

accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc gcgcatggcc     3120

gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct ggcgccgcac     3180

cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga ccaccagggc     3240

aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg cgccggggtg     3300

cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg gctcggcttc     3360

accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag     3420

cccggtgcct ga                                                         3432


<210>  2
<211>  486
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD19 aCAR Synthetic protein

<400>  2

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Gln Met Thr Gln Thr Thr Ser Ser Leu 
            20                  25                  30          


Ser Ala Ser Leu Gly Asp Arg Val Thr Ile Ser Cys Arg Ala Ser Gln 
        35                  40                  45              


Asp Ile Ser Lys Tyr Leu Asn Trp Tyr Gln Gln Lys Pro Asp Gly Thr 
    50                  55                  60                  


Val Lys Leu Leu Ile Tyr His Thr Ser Arg Leu His Ser Gly Val Pro 
65                  70                  75                  80  


Ser Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Tyr Ser Leu Thr Ile 
                85                  90                  95      


Ser Asn Leu Glu Gln Glu Asp Ile Ala Thr Tyr Phe Cys Gln Gln Gly 
            100                 105                 110         


Asn Thr Leu Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Thr 
        115                 120                 125             


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu 
    130                 135                 140                 


Val Lys Leu Gln Glu Ser Gly Pro Gly Leu Val Ala Pro Ser Gln Ser 
145                 150                 155                 160 


Leu Ser Val Thr Cys Thr Val Ser Gly Val Ser Leu Pro Asp Tyr Gly 
                165                 170                 175     


Val Ser Trp Ile Arg Gln Pro Pro Arg Lys Gly Leu Glu Trp Leu Gly 
            180                 185                 190         


Val Ile Trp Gly Ser Glu Thr Thr Tyr Tyr Asn Ser Ala Leu Lys Ser 
        195                 200                 205             


Arg Leu Thr Ile Ile Lys Asp Asn Ser Lys Ser Gln Val Phe Leu Lys 
    210                 215                 220                 


Met Asn Ser Leu Gln Thr Asp Asp Thr Ala Ile Tyr Tyr Cys Ala Lys 
225                 230                 235                 240 


His Tyr Tyr Tyr Gly Gly Ser Tyr Ala Met Asp Tyr Trp Gly Gln Gly 
                245                 250                 255     


Thr Ser Val Thr Val Ser Ser Thr Thr Thr Pro Ala Pro Arg Pro Pro 
            260                 265                 270         


Thr Pro Ala Pro Thr Ile Ala Ser Gln Pro Leu Ser Leu Arg Pro Glu 
        275                 280                 285             


Ala Cys Arg Pro Ala Ala Gly Gly Ala Val His Thr Arg Gly Leu Asp 
    290                 295                 300                 


Phe Ala Cys Asp Ile Tyr Ile Trp Ala Pro Leu Ala Gly Thr Cys Gly 
305                 310                 315                 320 


Val Leu Leu Leu Ser Leu Val Ile Thr Leu Tyr Cys Lys Arg Gly Arg 
                325                 330                 335     


Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro Phe Met Arg Pro Val Gln 
            340                 345                 350         


Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys Arg Phe Pro Glu Glu Glu 
        355                 360                 365             


Glu Gly Gly Cys Glu Leu Arg Val Lys Phe Ser Arg Ser Ala Asp Ala 
    370                 375                 380                 


Pro Ala Tyr Lys Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu 
385                 390                 395                 400 


Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp 
                405                 410                 415     


Pro Glu Met Gly Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu 
            420                 425                 430         


Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile 
        435                 440                 445             


Gly Met Lys Gly Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr 
    450                 455                 460                 


Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met 
465                 470                 475                 480 


Gln Ala Leu Pro Pro Arg 
                485     


<210>  3
<211>  237
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  RFP Synthetic Protein

<400>  3

Met Val Ser Lys Gly Glu Glu Leu Ile Lys Glu Asn Met His Met Lys 
1               5                   10                  15      


Leu Tyr Met Glu Gly Thr Val Asn Asn His His Phe Lys Cys Thr Ser 
            20                  25                  30          


Glu Gly Glu Gly Lys Pro Tyr Glu Gly Thr Gln Thr Met Arg Ile Lys 
        35                  40                  45              


Val Val Glu Gly Gly Pro Leu Pro Phe Ala Phe Asp Ile Leu Ala Thr 
    50                  55                  60                  


Ser Phe Met Tyr Gly Ser Arg Thr Phe Ile Asn His Thr Gln Gly Ile 
65                  70                  75                  80  


Pro Asp Phe Phe Lys Gln Ser Phe Pro Glu Gly Phe Thr Trp Glu Arg 
                85                  90                  95      


Val Thr Thr Tyr Glu Asp Gly Gly Val Leu Thr Ala Thr Gln Asp Thr 
            100                 105                 110         


Ser Leu Gln Asp Gly Cys Leu Ile Tyr Asn Val Lys Ile Arg Gly Val 
        115                 120                 125             


Asn Phe Pro Ser Asn Gly Pro Val Met Gln Lys Lys Thr Leu Gly Trp 
    130                 135                 140                 


Glu Ala Asn Thr Glu Met Leu Tyr Pro Ala Asp Gly Gly Leu Glu Gly 
145                 150                 155                 160 


Arg Ser Asp Met Ala Leu Lys Leu Val Gly Gly Gly His Leu Ile Cys 
                165                 170                 175     


Asn Phe Lys Thr Thr Tyr Arg Ser Lys Lys Pro Ala Lys Asn Leu Lys 
            180                 185                 190         


Met Pro Gly Val Tyr Tyr Val Asp His Arg Leu Glu Arg Ile Lys Glu 
        195                 200                 205             


Ala Asp Lys Glu Thr Tyr Val Glu Gln His Glu Val Ala Val Ala Arg 
    210                 215                 220                 


Tyr Cys Asp Leu Pro Ser Lys Leu Gly His Lys Leu Asn 
225                 230                 235         


<210>  4
<211>  200
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Puromycin resistance Synthetic Protein

<400>  4

Met Ala Thr Glu Tyr Lys Pro Thr Val Arg Leu Ala Thr Arg Asp Asp 
1               5                   10                  15      


Val Pro Arg Ala Val Arg Thr Leu Ala Ala Ala Phe Ala Asp Tyr Pro 
            20                  25                  30          


Ala Thr Arg His Thr Val Asp Pro Asp Arg His Ile Glu Arg Val Thr 
        35                  40                  45              


Glu Leu Gln Glu Leu Phe Leu Thr Arg Val Gly Leu Asp Ile Gly Lys 
    50                  55                  60                  


Val Trp Val Ala Asp Asp Gly Ala Ala Val Ala Val Trp Thr Thr Pro 
65                  70                  75                  80  


Glu Ser Val Glu Ala Gly Ala Val Phe Ala Glu Ile Gly Pro Arg Met 
                85                  90                  95      


Ala Glu Leu Ser Gly Ser Arg Leu Ala Ala Gln Gln Gln Met Glu Gly 
            100                 105                 110         


Leu Leu Ala Pro His Arg Pro Lys Glu Pro Ala Trp Phe Leu Ala Thr 
        115                 120                 125             


Val Gly Val Ser Pro Asp His Gln Gly Lys Gly Leu Gly Ser Ala Val 
    130                 135                 140                 


Val Leu Pro Gly Val Glu Ala Ala Glu Arg Ala Gly Val Pro Ala Phe 
145                 150                 155                 160 


Leu Glu Thr Ser Ala Pro Arg Asn Leu Pro Phe Tyr Glu Arg Leu Gly 
                165                 170                 175     


Phe Thr Val Thr Ala Asp Val Glu Val Pro Glu Gly Pro Arg Thr Trp 
            180                 185                 190         


Cys Met Thr Arg Lys Pro Gly Ala 
        195                 200 


<210>  5
<211>  3669
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CD20 iCAR_IRES_GFP_P2A_Hygro Synthetic DNA

<400>  5
atggcactgc ctgtgaccgc cctgctgctg ccactggccc tgctgctgca cgcagccagg       60

cccgacatcg tgctgacaca gagcccagca atcctgtccg cctctcctgg agagaaggtg      120

accatgacat gccgcgccag ctcctctgtg aactacatgg attggtatca gaagaagcct      180

ggcagctccc caaagccctg gatctacgcc accagcaatc tggcctccgg cgtgccagca      240

cggttcagcg gctccggctc tggcaccagc tattccctga caatctccag agtggaggca      300

gaggacgcag caacctacta ttgccagcag tggtctttca acccccctac ctttggcggc      360

ggcacaaagc tggagatcaa gggctctaca agcggaggag gctctggagg aggcagcgga      420

ggcggcggct ctagcgaggt gcagctgcag cagagcggag cagagctggt gaagcctgga      480

gcctccgtga agatgtcttg taaggccagc ggctacacct tcacatccta taatatgcac      540

tgggtgaagc agaccccagg acagggcctg gagtggatcg gagcaatcta cccaggaaac      600

ggcgacacaa gctataatca gaagtttaag ggcaaggcca ccctgacagc cgataagtcc      660

tctagcaccg cctacatgca gctgtcctct ctgacatccg aggactctgc cgattactat      720

tgtgcccggt ccaactacta tggcagctcc tactggttct ttgacgtgtg gggagcaggc      780

accacagtga ccgtgtctag caccgagagg agagcagagg tgcccacagc acacccatct      840

ccaagcccta ggccagcagg acagttccag accctggtgg tgggagtggt gggaggcctg      900

ctgggctctc tggtgctgct ggtgtgggtg ctggccgtga tctgcagcag ggccgcccgc      960

ggcaccatcg gcgccaggcg cacaggccag cctctgaagg aggacccttc cgccgtgcca     1020

gtgttctctg tggactacgg cgagctggat tttcagtggc gggagaaaac cccagagcca     1080

cctgtgccct gcgtgcctga gcagaccgag tatgccacaa tcgtgtttcc atccggaatg     1140

ggcacaagct cccctgcaag gagaggcagc gccgacggac cacggtccgc ccagccactg     1200

cggcccgagg atggccactg ttcttggccc ctgtgacgcc cctctccccc ccccccctct     1260

ccctcccccc cccctaacgt tactggccga agccgcttgg aataaggccg gtgtgcgttt     1320

gtctatatgt tattttccac catattgccg tcttttggca atgtgagggc ccggaaacct     1380

ggccctgtct tcttgacgag cattcctagg ggtctttccc ctctcgccaa aggaatgcaa     1440

ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag cttcttgaag acaaacaacg     1500

tctgtagcga ccctttgcag gcagcggaac cccccacctg gcgacaggtg cctctgcggc     1560

caaaagccac gtgtataaga tacacctgca aaggcggcac aaccccagtg ccacgttgtg     1620

agttggatag ttgtggaaag agtcaaatgg ctctcctcaa gcgtattcaa caaggggctg     1680

aaggatgccc agaaggtacc ccattgtatg ggatctgatc tggggcctcg gtgcacatgc     1740

tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc ccccgaacca cggggacgtg     1800

gttttccttt gaaaaacacg atgataaggc ttgccacaac ccgtaccaaa gatggtgtcc     1860

aagggagagg agctgttcac cggagtggtg cccatcctgg tggagctgga cggcgatgtg     1920

aatggccaca agtttagcgt gtccggagag ggagagggcg acgcaaccta cggcaagctg     1980

acactgaagt tcatctgcac cacaggcaag ctgcccgtgc cttggccaac cctggtgacc     2040

acactgacat acggcgtgca gtgtttttct cgctatcccg accacatgaa gcagcacgat     2100

ttctttaaga gcgccatgcc tgagggctac gtgcaggagc ggaccatctt ctttaaggac     2160

gatggcaact ataagaccag agccgaggtg aagttcgagg gcgacacact ggtgaacagg     2220

atcgagctga agggcatcga ctttaaggag gatggcaata tcctgggcca caagctggag     2280

tacaactata attcccacaa cgtgtacatc atggccgata agcagaagaa cggcatcaag     2340

gtcaatttca agatcagaca caatatcgag gacggctctg tgcagctggc cgatcactac     2400

cagcagaaca ccccaatcgg cgacggaccc gtgctgctgc ctgataatca ctatctgtct     2460

acacagagcg ccctgtccaa ggaccccaac gagaagaggg atcacatggt gctgctggag     2520

tttgtgaccg cagcaggaat cacactggga atggacgagc tgtataaggg cagcggcgcc     2580

accaacttct ccctgctgaa gcaggcaggc gacgtggagg agaatccagg acctatggat     2640

agaagcggca agccagagct gaccgccaca tccgtggaga agttcctgat cgagaagttt     2700

gactctgtga gcgatctgat gcagctgtcc gagggagagg agtccagggc cttctctttt     2760

gatgtgggcg gcaggggata cgtgctgagg gtgaatagct gcgccgacgg cttctataag     2820

gatagatacg tgtatagaca ctttgcctcc gccgccctgc caatcccaga ggtgctggac     2880

atcggcgagt tttccgagtc tctgacctac tgtatcagcc ggagagccca gggagtgacc     2940

ctgcaggatc tgcctgagac agagctgcca gccgtgctgc agccagtggc agaggctatg     3000

gacgcaatcg ccgccgccga cctgtctcag acaagcggct tcggcccttt tggcccacag     3060

ggcatcggcc agtacaccac atggagggac ttcatctgcg ccatcgccga tcctcacgtg     3120

tatcactggc agaccgtgat ggacgataca gtgagcgcct ccgtggcaca ggccctggac     3180

gagctgatgc tgtgggccga ggattgtcca gaggtgcgcc acctggtgca cgcagacttt     3240

ggcagcaaca atgtgctgac cgataatggc cggatcacag ccgtgatcga ctggtccgag     3300

gccatgttcg gcgattctca gtacgaggtg gccaacatct tcttttggag gccttggctg     3360

gcctgcatgg agcagcagac ccgctatttt gagaggcgcc accctgagct ggccggctct     3420

ccacggctga gagcatacat gctgcgcatc ggcctggacc agctgtatca gagcctggtg     3480

gatggcaatt tcgacgatgc agcatgggca cagggccggt gcgacgcaat cgtgagatcc     3540

ggcgccggca ccgtgggccg gacacagatc gcacggcgga gcgccgccgt gtggaccgac     3600

ggatgcgtgg aggtgctggc cgattctggc aacaggcgcc caagcacaag gccccgcgcc     3660

aaggagtga                                                             3669


<210>  6
<211>  411
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  CD20 iCAR Synthetic Protein

<400>  6

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Asp Ile Val Leu Thr Gln Ser Pro Ala Ile Leu 
            20                  25                  30          


Ser Ala Ser Pro Gly Glu Lys Val Thr Met Thr Cys Arg Ala Ser Ser 
        35                  40                  45              


Ser Val Asn Tyr Met Asp Trp Tyr Gln Lys Lys Pro Gly Ser Ser Pro 
    50                  55                  60                  


Lys Pro Trp Ile Tyr Ala Thr Ser Asn Leu Ala Ser Gly Val Pro Ala 
65                  70                  75                  80  


Arg Phe Ser Gly Ser Gly Ser Gly Thr Ser Tyr Ser Leu Thr Ile Ser 
                85                  90                  95      


Arg Val Glu Ala Glu Asp Ala Ala Thr Tyr Tyr Cys Gln Gln Trp Ser 
            100                 105                 110         


Phe Asn Pro Pro Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys Gly 
        115                 120                 125             


Ser Thr Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Gly Ser 
    130                 135                 140                 


Ser Glu Val Gln Leu Gln Gln Ser Gly Ala Glu Leu Val Lys Pro Gly 
145                 150                 155                 160 


Ala Ser Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser 
                165                 170                 175     


Tyr Asn Met His Trp Val Lys Gln Thr Pro Gly Gln Gly Leu Glu Trp 
            180                 185                 190         


Ile Gly Ala Ile Tyr Pro Gly Asn Gly Asp Thr Ser Tyr Asn Gln Lys 
        195                 200                 205             


Phe Lys Gly Lys Ala Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala 
    210                 215                 220                 


Tyr Met Gln Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Asp Tyr Tyr 
225                 230                 235                 240 


Cys Ala Arg Ser Asn Tyr Tyr Gly Ser Ser Tyr Trp Phe Phe Asp Val 
                245                 250                 255     


Trp Gly Ala Gly Thr Thr Val Thr Val Ser Ser Thr Glu Arg Arg Ala 
            260                 265                 270         


Glu Val Pro Thr Ala His Pro Ser Pro Ser Pro Arg Pro Ala Gly Gln 
        275                 280                 285             


Phe Gln Thr Leu Val Val Gly Val Val Gly Gly Leu Leu Gly Ser Leu 
    290                 295                 300                 


Val Leu Leu Val Trp Val Leu Ala Val Ile Cys Ser Arg Ala Ala Arg 
305                 310                 315                 320 


Gly Thr Ile Gly Ala Arg Arg Thr Gly Gln Pro Leu Lys Glu Asp Pro 
                325                 330                 335     


Ser Ala Val Pro Val Phe Ser Val Asp Tyr Gly Glu Leu Asp Phe Gln 
            340                 345                 350         


Trp Arg Glu Lys Thr Pro Glu Pro Pro Val Pro Cys Val Pro Glu Gln 
        355                 360                 365             


Thr Glu Tyr Ala Thr Ile Val Phe Pro Ser Gly Met Gly Thr Ser Ser 
    370                 375                 380                 


Pro Ala Arg Arg Gly Ser Ala Asp Gly Pro Arg Ser Ala Gln Pro Leu 
385                 390                 395                 400 


Arg Pro Glu Asp Gly His Cys Ser Trp Pro Leu 
                405                 410     


<210>  7
<211>  239
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  GFP Synthetic Protein

<400>  7

Met Val Ser Lys Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu 
1               5                   10                  15      


Val Glu Leu Asp Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly 
            20                  25                  30          


Glu Gly Glu Gly Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile 
        35                  40                  45              


Cys Thr Thr Gly Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr 
    50                  55                  60                  


Leu Thr Tyr Gly Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys 
65                  70                  75                  80  


Gln His Asp Phe Phe Lys Ser Ala Met Pro Glu Gly Tyr Val Gln Glu 
                85                  90                  95      


Arg Thr Ile Phe Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu 
            100                 105                 110         


Val Lys Phe Glu Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly 
        115                 120                 125             


Ile Asp Phe Lys Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr 
    130                 135                 140                 


Asn Tyr Asn Ser His Asn Val Tyr Ile Met Ala Asp Lys Gln Lys Asn 
145                 150                 155                 160 


Gly Ile Lys Val Asn Phe Lys Ile Arg His Asn Ile Glu Asp Gly Ser 
                165                 170                 175     


Val Gln Leu Ala Asp His Tyr Gln Gln Asn Thr Pro Ile Gly Asp Gly 
            180                 185                 190         


Pro Val Leu Leu Pro Asp Asn His Tyr Leu Ser Thr Gln Ser Ala Leu 
        195                 200                 205             


Ser Lys Asp Pro Asn Glu Lys Arg Asp His Met Val Leu Leu Glu Phe 
    210                 215                 220                 


Val Thr Ala Ala Gly Ile Thr Leu Gly Met Asp Glu Leu Tyr Lys 
225                 230                 235                 


<210>  8
<211>  344
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Hygromycin resistance Synthetic Protein

<400>  8

Met Asp Arg Ser Gly Lys Pro Glu Leu Thr Ala Thr Ser Val Glu Lys 
1               5                   10                  15      


Phe Leu Ile Glu Lys Phe Asp Ser Val Ser Asp Leu Met Gln Leu Ser 
            20                  25                  30          


Glu Gly Glu Glu Ser Arg Ala Phe Ser Phe Asp Val Gly Gly Arg Gly 
        35                  40                  45              


Tyr Val Leu Arg Val Asn Ser Cys Ala Asp Gly Phe Tyr Lys Asp Arg 
    50                  55                  60                  


Tyr Val Tyr Arg His Phe Ala Ser Ala Ala Leu Pro Ile Pro Glu Val 
65                  70                  75                  80  


Leu Asp Ile Gly Glu Phe Ser Glu Ser Leu Thr Tyr Cys Ile Ser Arg 
                85                  90                  95      


Arg Ala Gln Gly Val Thr Leu Gln Asp Leu Pro Glu Thr Glu Leu Pro 
            100                 105                 110         


Ala Val Leu Gln Pro Val Ala Glu Ala Met Asp Ala Ile Ala Ala Ala 
        115                 120                 125             


Asp Leu Ser Gln Thr Ser Gly Phe Gly Pro Phe Gly Pro Gln Gly Ile 
    130                 135                 140                 


Gly Gln Tyr Thr Thr Trp Arg Asp Phe Ile Cys Ala Ile Ala Asp Pro 
145                 150                 155                 160 


His Val Tyr His Trp Gln Thr Val Met Asp Asp Thr Val Ser Ala Ser 
                165                 170                 175     


Val Ala Gln Ala Leu Asp Glu Leu Met Leu Trp Ala Glu Asp Cys Pro 
            180                 185                 190         


Glu Val Arg His Leu Val His Ala Asp Phe Gly Ser Asn Asn Val Leu 
        195                 200                 205             


Thr Asp Asn Gly Arg Ile Thr Ala Val Ile Asp Trp Ser Glu Ala Met 
    210                 215                 220                 


Phe Gly Asp Ser Gln Tyr Glu Val Ala Asn Ile Phe Phe Trp Arg Pro 
225                 230                 235                 240 


Trp Leu Ala Cys Met Glu Gln Gln Thr Arg Tyr Phe Glu Arg Arg His 
                245                 250                 255     


Pro Glu Leu Ala Gly Ser Pro Arg Leu Arg Ala Tyr Met Leu Arg Ile 
            260                 265                 270         


Gly Leu Asp Gln Leu Tyr Gln Ser Leu Val Asp Gly Asn Phe Asp Asp 
        275                 280                 285             


Ala Ala Trp Ala Gln Gly Arg Cys Asp Ala Ile Val Arg Ser Gly Ala 
    290                 295                 300                 


Gly Thr Val Gly Arg Thr Gln Ile Ala Arg Arg Ser Ala Ala Val Trp 
305                 310                 315                 320 


Thr Asp Gly Cys Val Glu Val Leu Ala Asp Ser Gly Asn Arg Arg Pro 
                325                 330                 335     


Ser Thr Arg Pro Arg Ala Lys Glu 
            340                 


<210>  9
<211>  3696
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  HLA-A2 iCAR_IRES_ GFP_P2A_Hygro Synthetic DNA

<400>  9
atggcactgc cagtgaccgc cctgctgctg cctctggccc tgctgctgca cgcagccaga       60

cccgagcaga agctgatctc cgaggaggac ctgcaggtgc agctgcagca gtctggacct      120

gagctggtga agccaggagc ctccgtgaag atgtcttgca aggccagcgg ctacaccttc      180

acatcttatc acatccagtg ggtgaagcag cggcccggac agggcctgga gtggatcgga      240

tggatctacc caggcgacgg ctccacacag tataacgaga agttcaaggg caagaccaca      300

ctgaccgccg ataagagcag cagcaccgcc tacatgctgc tgagcagcct gaccagcgag      360

gacagcgcca tctacttttg cgccagggag ggcacatact atgctatgga ctattggggc      420

cagggcacca gcgtgacagt gtctagcgga ggaggaggct ccggaggagg aggctctggc      480

ggcggcggca gcgacgtgct gatgacccag acaccactga gcctgcccgt gagcctgggc      540

gatcaggtga gcatctcctg tagatcctct cagagcatcg tgcactccaa cggcaatacc      600

tacctggagt ggtatctgca gaagccaggc cagtccccca agctgctgat ctataaggtg      660

tctaatcggt tcagcggcgt gcctgacaga ttttctggca gcggctccgg caccgacttc      720

accctgaaga tcagccgggt ggaggcagag gatctgggcg tgtactattg tttccagggc      780

tcccacgtgc cacgcacctt tggcggcggc acaaagctgg agatcaagac cgagaggaga      840

gcagaggtgc ccacagcaca cccatctcca agccctaggc cagcaggaca gttccagacc      900

ctggtggtgg gagtggtggg aggcctgctg ggctctctgg tgctgctggt gtgggtgctg      960

gccgtgatct gcagcagggc cgcccgcggc accatcggcg ccaggcgcac aggccagcct     1020

ctgaaggagg acccttccgc cgtgccagtg ttctctgtgg actacggcga gctggatttt     1080

cagtggcggg agaaaacccc agagccacct gtgccctgcg tgcctgagca gaccgagtat     1140

gccacaatcg tgtttccatc cggaatgggc acaagctccc ctgcaaggag aggcagcgcc     1200

gacggaccac ggtccgccca gccactgcgg cccgaggatg gccactgttc ttggcccctg     1260

tgacgcccct ctcccccccc cccctctccc tccccccccc ctaacgttac tggccgaagc     1320

cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct     1380

tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt     1440

ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct     1500

ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc     1560

ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag     1620

gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc     1680

tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga     1740

tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt     1800

ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataaggcttg     1860

ccacaacccg taccaaagat ggtgtccaag ggagaggagc tgttcaccgg agtggtgccc     1920

atcctggtgg agctggacgg cgatgtgaat ggccacaagt ttagcgtgtc cggagaggga     1980

gagggcgacg caacctacgg caagctgaca ctgaagttca tctgcaccac aggcaagctg     2040

cccgtgcctt ggccaaccct ggtgaccaca ctgacatacg gcgtgcagtg tttttctcgc     2100

tatcccgacc acatgaagca gcacgatttc tttaagagcg ccatgcctga gggctacgtg     2160

caggagcgga ccatcttctt taaggacgat ggcaactata agaccagagc cgaggtgaag     2220

ttcgagggcg acacactggt gaacaggatc gagctgaagg gcatcgactt taaggaggat     2280

ggcaatatcc tgggccacaa gctggagtac aactataatt cccacaacgt gtacatcatg     2340

gccgataagc agaagaacgg catcaaggtc aatttcaaga tcagacacaa tatcgaggac     2400

ggctctgtgc agctggccga tcactaccag cagaacaccc caatcggcga cggacccgtg     2460

ctgctgcctg ataatcacta tctgtctaca cagagcgccc tgtccaagga ccccaacgag     2520

aagagggatc acatggtgct gctggagttt gtgaccgcag caggaatcac actgggaatg     2580

gacgagctgt ataagggcag cggcgccacc aacttctccc tgctgaagca ggcaggcgac     2640

gtggaggaga atccaggacc tatggataga agcggcaagc cagagctgac cgccacatcc     2700

gtggagaagt tcctgatcga gaagtttgac tctgtgagcg atctgatgca gctgtccgag     2760

ggagaggagt ccagggcctt ctcttttgat gtgggcggca ggggatacgt gctgagggtg     2820

aatagctgcg ccgacggctt ctataaggat agatacgtgt atagacactt tgcctccgcc     2880

gccctgccaa tcccagaggt gctggacatc ggcgagtttt ccgagtctct gacctactgt     2940

atcagccgga gagcccaggg agtgaccctg caggatctgc ctgagacaga gctgccagcc     3000

gtgctgcagc cagtggcaga ggctatggac gcaatcgccg ccgccgacct gtctcagaca     3060

agcggcttcg gcccttttgg cccacagggc atcggccagt acaccacatg gagggacttc     3120

atctgcgcca tcgccgatcc tcacgtgtat cactggcaga ccgtgatgga cgatacagtg     3180

agcgcctccg tggcacaggc cctggacgag ctgatgctgt gggccgagga ttgtccagag     3240

gtgcgccacc tggtgcacgc agactttggc agcaacaatg tgctgaccga taatggccgg     3300

atcacagccg tgatcgactg gtccgaggcc atgttcggcg attctcagta cgaggtggcc     3360

aacatcttct tttggaggcc ttggctggcc tgcatggagc agcagacccg ctattttgag     3420

aggcgccacc ctgagctggc cggctctcca cggctgagag catacatgct gcgcatcggc     3480

ctggaccagc tgtatcagag cctggtggat ggcaatttcg acgatgcagc atgggcacag     3540

ggccggtgcg acgcaatcgt gagatccggc gccggcaccg tgggccggac acagatcgca     3600

cggcggagcg ccgccgtgtg gaccgacgga tgcgtggagg tgctggccga ttctggcaac     3660

aggcgcccaa gcacaaggcc ccgcgccaag gagtga                               3696


<210>  10
<211>  420
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA-A2 iCAR Synthetic Protein

<400>  10

Met Ala Leu Pro Val Thr Ala Leu Leu Leu Pro Leu Ala Leu Leu Leu 
1               5                   10                  15      


His Ala Ala Arg Pro Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu Gln 
            20                  25                  30          


Val Gln Leu Gln Gln Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
        35                  40                  45              


Val Lys Met Ser Cys Lys Ala Ser Gly Tyr Thr Phe Thr Ser Tyr His 
    50                  55                  60                  


Ile Gln Trp Val Lys Gln Arg Pro Gly Gln Gly Leu Glu Trp Ile Gly 
65                  70                  75                  80  


Trp Ile Tyr Pro Gly Asp Gly Ser Thr Gln Tyr Asn Glu Lys Phe Lys 
                85                  90                  95      


Gly Lys Thr Thr Leu Thr Ala Asp Lys Ser Ser Ser Thr Ala Tyr Met 
            100                 105                 110         


Leu Leu Ser Ser Leu Thr Ser Glu Asp Ser Ala Ile Tyr Phe Cys Ala 
        115                 120                 125             


Arg Glu Gly Thr Tyr Tyr Ala Met Asp Tyr Trp Gly Gln Gly Thr Ser 
    130                 135                 140                 


Val Thr Val Ser Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
145                 150                 155                 160 


Gly Gly Gly Ser Asp Val Leu Met Thr Gln Thr Pro Leu Ser Leu Pro 
                165                 170                 175     


Val Ser Leu Gly Asp Gln Val Ser Ile Ser Cys Arg Ser Ser Gln Ser 
            180                 185                 190         


Ile Val His Ser Asn Gly Asn Thr Tyr Leu Glu Trp Tyr Leu Gln Lys 
        195                 200                 205             


Pro Gly Gln Ser Pro Lys Leu Leu Ile Tyr Lys Val Ser Asn Arg Phe 
    210                 215                 220                 


Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe 
225                 230                 235                 240 


Thr Leu Lys Ile Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr 
                245                 250                 255     


Cys Phe Gln Gly Ser His Val Pro Arg Thr Phe Gly Gly Gly Thr Lys 
            260                 265                 270         


Leu Glu Ile Lys Thr Glu Arg Arg Ala Glu Val Pro Thr Ala His Pro 
        275                 280                 285             


Ser Pro Ser Pro Arg Pro Ala Gly Gln Phe Gln Thr Leu Val Val Gly 
    290                 295                 300                 


Val Val Gly Gly Leu Leu Gly Ser Leu Val Leu Leu Val Trp Val Leu 
305                 310                 315                 320 


Ala Val Ile Cys Ser Arg Ala Ala Arg Gly Thr Ile Gly Ala Arg Arg 
                325                 330                 335     


Thr Gly Gln Pro Leu Lys Glu Asp Pro Ser Ala Val Pro Val Phe Ser 
            340                 345                 350         


Val Asp Tyr Gly Glu Leu Asp Phe Gln Trp Arg Glu Lys Thr Pro Glu 
        355                 360                 365             


Pro Pro Val Pro Cys Val Pro Glu Gln Thr Glu Tyr Ala Thr Ile Val 
    370                 375                 380                 


Phe Pro Ser Gly Met Gly Thr Ser Ser Pro Ala Arg Arg Gly Ser Ala 
385                 390                 395                 400 


Asp Gly Pro Arg Ser Ala Gln Pro Leu Arg Pro Glu Asp Gly His Cys 
                405                 410                 415     


Ser Trp Pro Leu 
            420 


<210>  11
<211>  43
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  11
aatgatacgg cgaccaccga gatctacact cgtcggcagc gtc                         43


<210>  12
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic sequence

<400>  12
caagcagaag acggcatacg agatgtctcg tgggctcgg                              39


