﻿               SEQUENCE LISTING

<110> Université d'Aix-Marseille
      Institut national de la santé et de la recherche médicale
      Assistance Publique - Hôpitaux de Marseille

<120> ANTI-CD146 ANTIBODIES AND USES THEREOF

<130> CD146 tumoral - 639

<160> 18

<170> BiSSAP 1.3.6

<210> 1
<211> 6
<212> PRT
<213> artificial sequence


<220> 
<223> Light Chain CDR1

<400> 1
Gln Asn Ile Tyr Lys Asn 
1               5       

<210> 2
<211> 8
<212> PRT
<213> artificial sequence


<220> 
<223> Light Chain CDR3

<400> 2
Gln Gln Tyr Tyr Ser Gly Trp Thr 
1               5               

<210> 3
<211> 9
<212> PRT
<213> artificial sequence


<220> 
<223> Heavy Chain CDR 4

<400> 3
Ser Gly Phe Thr Phe Ser Asn Tyr Gly 
1               5                   

<210> 4
<211> 8
<212> PRT
<213> artificial sequence


<220> 
<223> Heavy Chain CDR 5

<400> 4
Tyr Ile Ser His Asp Gly Gly Ser 
1               5               

<210> 5
<211> 10
<212> PRT
<213> artificial sequence


<220> 
<223> Heavy Chain CDR 6

<400> 5
Asp Thr Thr Asp Tyr Tyr Phe Asp Tyr Trp 
1               5                   10  

<210> 6
<211> 106
<212> PRT
<213> artificial sequence


<220> 
<223> Full VL region

<400> 6
Asp Ile Gln Met Thr Gln Ser Pro Ser Leu Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Leu Asn Cys Lys Ala Ser Gln Asn Ile Tyr Lys Asn 
            20                  25                  30          
Leu Ala Trp Tyr Gln Gln Lys Leu Gly Glu Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Asn Ala Asn Ser Leu Gln Thr Gly Ile Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Glu Leu Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Val Ala Thr Tyr Phe Cys Gln Gln Tyr Tyr Ser Gly Trp Thr 
                85                  90                  95      
Phe Gly Gly Gly Thr Lys Leu Glu Leu Lys 
            100                 105     

<210> 7
<211> 118
<212> PRT
<213> artificial sequence


<220> 
<223> VH full region

<400> 7
Glu Glu Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Arg 
1               5                   10                  15      
Ser Met Lys Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asn Tyr 
            20                  25                  30          
Gly Met Ala Trp Val Arg Gln Ala Pro Lys Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Tyr Ile Ser His Asp Gly Gly Ser Thr Tyr Tyr Arg Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Ser Thr Leu Tyr 
65                  70                  75                  80  
Leu Gln Met Asp Ser Leu Arg Ser Glu Asp Thr Ala Thr Tyr Tyr Cys 
                85                  90                  95      
Thr Thr Asp Thr Thr Asp Tyr Tyr Phe Asp Tyr Trp Gly Gln Gly Val 
            100                 105                 110         
Met Val Thr Val Ser Ser 
        115             

<210> 8
<211> 552
<212> PRT
<213> Homo sapiens


<220> 
<223> CD146 sequence 1

<400> 8
Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      
Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          
Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              
Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  
Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  
Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      
Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         
Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             
Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 
Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 
Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     
Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         
Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             
Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 
Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 
Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     
Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         
Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             
Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 
Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 
Gln Ala Trp Asn Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     
Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         
Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             
Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Asp Gln 
    370                 375                 380                 
Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 
Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     
Leu Asn Arg Thr Gln Leu Val Lys Leu Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         
Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             
Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 
Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 
Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     
Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         
Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             
Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 
Ser Thr Ser Thr Glu Arg Lys Leu 
545                 550         

<210> 9
<211> 553
<212> PRT
<213> Homo sapiens


<220> 
<223> CD146 sequence 2

<400> 9
Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      
Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          
Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              
Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  
Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  
Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      
Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         
Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             
Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 
Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 
Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     
Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         
Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             
Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 
Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 
Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     
Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         
Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             
Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 
Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 
Gln Ala Trp Asn Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     
Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         
Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             
Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Asp Gln 
    370                 375                 380                 
Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 
Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     
Leu Asn Arg Thr Gln Leu Val Lys Leu Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         
Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             
Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 
Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 
Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     
Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         
Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             
Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 
Ser Thr Ser Thr Glu Arg Lys Leu Pro 
545                 550             

<210> 10
<211> 554
<212> PRT
<213> Homo sapiens


<220> 
<223> CD146 sequence 3

<400> 10
Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      
Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          
Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              
Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  
Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  
Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      
Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         
Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             
Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 
Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 
Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     
Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         
Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             
Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 
Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 
Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     
Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         
Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             
Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 
Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 
Gln Ala Trp Asn Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     
Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         
Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             
Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Asp Gln 
    370                 375                 380                 
Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 
Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     
Leu Asn Arg Thr Gln Leu Val Lys Leu Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         
Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             
Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 
Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 
Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     
Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         
Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             
Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 
Ser Thr Ser Thr Glu Arg Lys Leu Pro Glu 
545                 550                 

<210> 11
<211> 555
<212> PRT
<213> Homo sapiens


<220> 
<223> CD146 sequence 4

<400> 11
Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      
Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          
Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              
Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  
Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  
Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      
Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         
Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             
Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 
Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 
Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     
Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         
Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             
Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 
Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 
Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     
Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         
Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             
Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 
Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 
Gln Ala Trp Asn Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     
Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         
Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             
Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Asp Gln 
    370                 375                 380                 
Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 
Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     
Leu Asn Arg Thr Gln Leu Val Lys Leu Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         
Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             
Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 
Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 
Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     
Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         
Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             
Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 
Ser Thr Ser Thr Glu Arg Lys Leu Pro Glu Pro 
545                 550                 555 

<210> 12
<211> 556
<212> PRT
<213> Homo sapiens


<220> 
<223> CD146 sequence 5

<400> 12
Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      
Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          
Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              
Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  
Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  
Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      
Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         
Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             
Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 
Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 
Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     
Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         
Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             
Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 
Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 
Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     
Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         
Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             
Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 
Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 
Gln Ala Trp Asn Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     
Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         
Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             
Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Asp Gln 
    370                 375                 380                 
Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 
Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     
Leu Asn Arg Thr Gln Leu Val Lys Leu Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         
Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             
Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 
Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 
Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     
Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         
Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             
Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 
Ser Thr Ser Thr Glu Arg Lys Leu Pro Glu Pro Glu 
545                 550                 555     

<210> 13
<211> 557
<212> PRT
<213> Homo sapiens


<220> 
<223> CD146 sequence 6

<400> 13
Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      
Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          
Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              
Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  
Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  
Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      
Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         
Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             
Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 
Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 
Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     
Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         
Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             
Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 
Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 
Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     
Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         
Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             
Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 
Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 
Gln Ala Trp Asn Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     
Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         
Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             
Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Asp Gln 
    370                 375                 380                 
Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 
Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     
Leu Asn Arg Thr Gln Leu Val Lys Leu Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         
Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             
Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 
Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 
Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     
Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         
Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             
Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 
Ser Thr Ser Thr Glu Arg Lys Leu Pro Glu Pro Glu Ser 
545                 550                 555         

<210> 14
<211> 558
<212> PRT
<213> Homo sapiens


<220> 
<223> CD146 sequence 7

<400> 14
Met Gly Leu Pro Arg Leu Val Cys Ala Phe Leu Leu Ala Ala Cys Cys 
1               5                   10                  15      
Cys Cys Pro Arg Val Ala Gly Val Pro Gly Glu Ala Glu Gln Pro Ala 
            20                  25                  30          
Pro Glu Leu Val Glu Val Glu Val Gly Ser Thr Ala Leu Leu Lys Cys 
        35                  40                  45              
Gly Leu Ser Gln Ser Gln Gly Asn Leu Ser His Val Asp Trp Phe Ser 
    50                  55                  60                  
Val His Lys Glu Lys Arg Thr Leu Ile Phe Arg Val Arg Gln Gly Gln 
65                  70                  75                  80  
Gly Gln Ser Glu Pro Gly Glu Tyr Glu Gln Arg Leu Ser Leu Gln Asp 
                85                  90                  95      
Arg Gly Ala Thr Leu Ala Leu Thr Gln Val Thr Pro Gln Asp Glu Arg 
            100                 105                 110         
Ile Phe Leu Cys Gln Gly Lys Arg Pro Arg Ser Gln Glu Tyr Arg Ile 
        115                 120                 125             
Gln Leu Arg Val Tyr Lys Ala Pro Glu Glu Pro Asn Ile Gln Val Asn 
    130                 135                 140                 
Pro Leu Gly Ile Pro Val Asn Ser Lys Glu Pro Glu Glu Val Ala Thr 
145                 150                 155                 160 
Cys Val Gly Arg Asn Gly Tyr Pro Ile Pro Gln Val Ile Trp Tyr Lys 
                165                 170                 175     
Asn Gly Arg Pro Leu Lys Glu Glu Lys Asn Arg Val His Ile Gln Ser 
            180                 185                 190         
Ser Gln Thr Val Glu Ser Ser Gly Leu Tyr Thr Leu Gln Ser Ile Leu 
        195                 200                 205             
Lys Ala Gln Leu Val Lys Glu Asp Lys Asp Ala Gln Phe Tyr Cys Glu 
    210                 215                 220                 
Leu Asn Tyr Arg Leu Pro Ser Gly Asn His Met Lys Glu Ser Arg Glu 
225                 230                 235                 240 
Val Thr Val Pro Val Phe Tyr Pro Thr Glu Lys Val Trp Leu Glu Val 
                245                 250                 255     
Glu Pro Val Gly Met Leu Lys Glu Gly Asp Arg Val Glu Ile Arg Cys 
            260                 265                 270         
Leu Ala Asp Gly Asn Pro Pro Pro His Phe Ser Ile Ser Lys Gln Asn 
        275                 280                 285             
Pro Ser Thr Arg Glu Ala Glu Glu Glu Thr Thr Asn Asp Asn Gly Val 
    290                 295                 300                 
Leu Val Leu Glu Pro Ala Arg Lys Glu His Ser Gly Arg Tyr Glu Cys 
305                 310                 315                 320 
Gln Ala Trp Asn Leu Asp Thr Met Ile Ser Leu Leu Ser Glu Pro Gln 
                325                 330                 335     
Glu Leu Leu Val Asn Tyr Val Ser Asp Val Arg Val Ser Pro Ala Ala 
            340                 345                 350         
Pro Glu Arg Gln Glu Gly Ser Ser Leu Thr Leu Thr Cys Glu Ala Glu 
        355                 360                 365             
Ser Ser Gln Asp Leu Glu Phe Gln Trp Leu Arg Glu Glu Thr Asp Gln 
    370                 375                 380                 
Val Leu Glu Arg Gly Pro Val Leu Gln Leu His Asp Leu Lys Arg Glu 
385                 390                 395                 400 
Ala Gly Gly Gly Tyr Arg Cys Val Ala Ser Val Pro Ser Ile Pro Gly 
                405                 410                 415     
Leu Asn Arg Thr Gln Leu Val Lys Leu Ala Ile Phe Gly Pro Pro Trp 
            420                 425                 430         
Met Ala Phe Lys Glu Arg Lys Val Trp Val Lys Glu Asn Met Val Leu 
        435                 440                 445             
Asn Leu Ser Cys Glu Ala Ser Gly His Pro Arg Pro Thr Ile Ser Trp 
    450                 455                 460                 
Asn Val Asn Gly Thr Ala Ser Glu Gln Asp Gln Asp Pro Gln Arg Val 
465                 470                 475                 480 
Leu Ser Thr Leu Asn Val Leu Val Thr Pro Glu Leu Leu Glu Thr Gly 
                485                 490                 495     
Val Glu Cys Thr Ala Ser Asn Asp Leu Gly Lys Asn Thr Ser Ile Leu 
            500                 505                 510         
Phe Leu Glu Leu Val Asn Leu Thr Thr Leu Thr Pro Asp Ser Asn Thr 
        515                 520                 525             
Thr Thr Gly Leu Ser Thr Ser Thr Ala Ser Pro His Thr Arg Ala Asn 
    530                 535                 540                 
Ser Thr Ser Thr Glu Arg Lys Leu Pro Glu Pro Glu Ser Arg 
545                 550                 555             

<210> 15
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> GAPDH Reverse Primer Sequence

<400> 15
ggtggtctcc tgacttcaac a                                              21


<210> 16
<211> 22
<212> DNA
<213> Artificial Sequence


<220> 
<223> GAPDH Forward Primer Sequence

<400> 16
gttgctgtag ccaattcgtt gt                                             22


<210> 17
<211> 21
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD146 Reverse Primer

<400> 17
ggctaatgcc tcagatcgat g                                              21


<210> 18
<211> 23
<212> DNA
<213> Artificial Sequence


<220> 
<223> CD146 Forward Primer

<400> 18
aatatggtgt tgaatctgtc ttg                                            23


