                         SEQUENCE LISTING

<110>  Universitt Heidelberg
       Deutsches Krebsforschungszentrum
 
<120>  Modulated Cas-Inhibitors

<130>  UH14798PC

<150>  EP17196813.4
<151>  2017-10-17

<160>  176   

<170>  PatentIn version 3.3

<210>  1
<211>  87
<212>  PRT
<213>  Listeria monocytogenes

<400>  1

Met Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr 
1               5                   10                  15      


Val Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile 
            20                  25                  30          


Arg Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn 
        35                  40                  45              


Glu Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn 
    50                  55                  60                  


Gln Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile 
65                  70                  75                  80  


Thr Leu Lys Ser Glu Leu Asn 
                85          


<210>  2
<211>  261
<212>  DNA
<213>  Listeria monocytogenes

<400>  2
atgaacatta acgacctgat tcgggaaata aagaacaagg actacacggt caagctgtca     60

ggcaccgact ccaactctat aacacagctc attataaggg ttaataatga tggcaacgaa    120

tatgtcatct ctgaatccga gaatgaatca atcgtggaga aattcatcag tgcatttaaa    180

aacggctgga accaggagta cgaagatgag gaagagttct acaatgatat gcaaactatc    240

acactgaaat ctgagctgaa c                                              261


<210>  3
<211>  7
<212>  PRT
<213>  Simian virus 40

<400>  3

Pro Lys Lys Lys Arg Lys Val 
1               5           


<210>  4
<211>  21
<212>  DNA
<213>  Simian virus 40

<400>  4
ccaaagaaga agcggaaggt c                                               21


<210>  5
<211>  1367
<212>  PRT
<213>  Artificial

<220>
<223>  Cas9

<400>  5

Asp Lys Lys Tyr Ser Ile Gly Leu Asp Ile Gly Thr Asn Ser Val Gly 
1               5                   10                  15      


Trp Ala Val Ile Thr Asp Glu Tyr Lys Val Pro Ser Lys Lys Phe Lys 
            20                  25                  30          


Val Leu Gly Asn Thr Asp Arg His Ser Ile Lys Lys Asn Leu Ile Gly 
        35                  40                  45              


Ala Leu Leu Phe Asp Ser Gly Glu Thr Ala Glu Ala Thr Arg Leu Lys 
    50                  55                  60                  


Arg Thr Ala Arg Arg Arg Tyr Thr Arg Arg Lys Asn Arg Ile Cys Tyr 
65                  70                  75                  80  


Leu Gln Glu Ile Phe Ser Asn Glu Met Ala Lys Val Asp Asp Ser Phe 
                85                  90                  95      


Phe His Arg Leu Glu Glu Ser Phe Leu Val Glu Glu Asp Lys Lys His 
            100                 105                 110         


Glu Arg His Pro Ile Phe Gly Asn Ile Val Asp Glu Val Ala Tyr His 
        115                 120                 125             


Glu Lys Tyr Pro Thr Ile Tyr His Leu Arg Lys Lys Leu Val Asp Ser 
    130                 135                 140                 


Thr Asp Lys Ala Asp Leu Arg Leu Ile Tyr Leu Ala Leu Ala His Met 
145                 150                 155                 160 


Ile Lys Phe Arg Gly His Phe Leu Ile Glu Gly Asp Leu Asn Pro Asp 
                165                 170                 175     


Asn Ser Asp Val Asp Lys Leu Phe Ile Gln Leu Val Gln Thr Tyr Asn 
            180                 185                 190         


Gln Leu Phe Glu Glu Asn Pro Ile Asn Ala Ser Gly Val Asp Ala Lys 
        195                 200                 205             


Ala Ile Leu Ser Ala Arg Leu Ser Lys Ser Arg Arg Leu Glu Asn Leu 
    210                 215                 220                 


Ile Ala Gln Leu Pro Gly Glu Lys Lys Asn Gly Leu Phe Gly Asn Leu 
225                 230                 235                 240 


Ile Ala Leu Ser Leu Gly Leu Thr Pro Asn Phe Lys Ser Asn Phe Asp 
                245                 250                 255     


Leu Ala Glu Asp Ala Lys Leu Gln Leu Ser Lys Asp Thr Tyr Asp Asp 
            260                 265                 270         


Asp Leu Asp Asn Leu Leu Ala Gln Ile Gly Asp Gln Tyr Ala Asp Leu 
        275                 280                 285             


Phe Leu Ala Ala Lys Asn Leu Ser Asp Ala Ile Leu Leu Ser Asp Ile 
    290                 295                 300                 


Leu Arg Val Asn Thr Glu Ile Thr Lys Ala Pro Leu Ser Ala Ser Met 
305                 310                 315                 320 


Ile Lys Arg Tyr Asp Glu His His Gln Asp Leu Thr Leu Leu Lys Ala 
                325                 330                 335     


Leu Val Arg Gln Gln Leu Pro Glu Lys Tyr Lys Glu Ile Phe Phe Asp 
            340                 345                 350         


Gln Ser Lys Asn Gly Tyr Ala Gly Tyr Ile Asp Gly Gly Ala Ser Gln 
        355                 360                 365             


Glu Glu Phe Tyr Lys Phe Ile Lys Pro Ile Leu Glu Lys Met Asp Gly 
    370                 375                 380                 


Thr Glu Glu Leu Leu Val Lys Leu Asn Arg Glu Asp Leu Leu Arg Lys 
385                 390                 395                 400 


Gln Arg Thr Phe Asp Asn Gly Ser Ile Pro His Gln Ile His Leu Gly 
                405                 410                 415     


Glu Leu His Ala Ile Leu Arg Arg Gln Glu Asp Phe Tyr Pro Phe Leu 
            420                 425                 430         


Lys Asp Asn Arg Glu Lys Ile Glu Lys Ile Leu Thr Phe Arg Ile Pro 
        435                 440                 445             


Tyr Tyr Val Gly Pro Leu Ala Arg Gly Asn Ser Arg Phe Ala Trp Met 
    450                 455                 460                 


Thr Arg Lys Ser Glu Glu Thr Ile Thr Pro Trp Asn Phe Glu Glu Val 
465                 470                 475                 480 


Val Asp Lys Gly Ala Ser Ala Gln Ser Phe Ile Glu Arg Met Thr Asn 
                485                 490                 495     


Phe Asp Lys Asn Leu Pro Asn Glu Lys Val Leu Pro Lys His Ser Leu 
            500                 505                 510         


Leu Tyr Glu Tyr Phe Thr Val Tyr Asn Glu Leu Thr Lys Val Lys Tyr 
        515                 520                 525             


Val Thr Glu Gly Met Arg Lys Pro Ala Phe Leu Ser Gly Glu Gln Lys 
    530                 535                 540                 


Lys Ala Ile Val Asp Leu Leu Phe Lys Thr Asn Arg Lys Val Thr Val 
545                 550                 555                 560 


Lys Gln Leu Lys Glu Asp Tyr Phe Lys Lys Ile Glu Cys Phe Asp Ser 
                565                 570                 575     


Val Glu Ile Ser Gly Val Glu Asp Arg Phe Asn Ala Ser Leu Gly Thr 
            580                 585                 590         


Tyr His Asp Leu Leu Lys Ile Ile Lys Asp Lys Asp Phe Leu Asp Asn 
        595                 600                 605             


Glu Glu Asn Glu Asp Ile Leu Glu Asp Ile Val Leu Thr Leu Thr Leu 
    610                 615                 620                 


Phe Glu Asp Arg Glu Met Ile Glu Glu Arg Leu Lys Thr Tyr Ala His 
625                 630                 635                 640 


Leu Phe Asp Asp Lys Val Met Lys Gln Leu Lys Arg Arg Arg Tyr Thr 
                645                 650                 655     


Gly Trp Gly Arg Leu Ser Arg Lys Leu Ile Asn Gly Ile Arg Asp Lys 
            660                 665                 670         


Gln Ser Gly Lys Thr Ile Leu Asp Phe Leu Lys Ser Asp Gly Phe Ala 
        675                 680                 685             


Asn Arg Asn Phe Met Gln Leu Ile His Asp Asp Ser Leu Thr Phe Lys 
    690                 695                 700                 


Glu Asp Ile Gln Lys Ala Gln Val Ser Gly Gln Gly Asp Ser Leu His 
705                 710                 715                 720 


Glu His Ile Ala Asn Leu Ala Gly Ser Pro Ala Ile Lys Lys Gly Ile 
                725                 730                 735     


Leu Gln Thr Val Lys Val Val Asp Glu Leu Val Lys Val Met Gly Arg 
            740                 745                 750         


His Lys Pro Glu Asn Ile Val Ile Glu Met Ala Arg Glu Asn Gln Thr 
        755                 760                 765             


Thr Gln Lys Gly Gln Lys Asn Ser Arg Glu Arg Met Lys Arg Ile Glu 
    770                 775                 780                 


Glu Gly Ile Lys Glu Leu Gly Ser Gln Ile Leu Lys Glu His Pro Val 
785                 790                 795                 800 


Glu Asn Thr Gln Leu Gln Asn Glu Lys Leu Tyr Leu Tyr Tyr Leu Gln 
                805                 810                 815     


Asn Gly Arg Asp Met Tyr Val Asp Gln Glu Leu Asp Ile Asn Arg Leu 
            820                 825                 830         


Ser Asp Tyr Asp Val Asp His Ile Val Pro Gln Ser Phe Leu Lys Asp 
        835                 840                 845             


Asp Ser Ile Asp Asn Lys Val Leu Thr Arg Ser Asp Lys Asn Arg Gly 
    850                 855                 860                 


Lys Ser Asp Asn Val Pro Ser Glu Glu Val Val Lys Lys Met Lys Asn 
865                 870                 875                 880 


Tyr Trp Arg Gln Leu Leu Asn Ala Lys Leu Ile Thr Gln Arg Lys Phe 
                885                 890                 895     


Asp Asn Leu Thr Lys Ala Glu Arg Gly Gly Leu Ser Glu Leu Asp Lys 
            900                 905                 910         


Ala Gly Phe Ile Lys Arg Gln Leu Val Glu Thr Arg Gln Ile Thr Lys 
        915                 920                 925             


His Val Ala Gln Ile Leu Asp Ser Arg Met Asn Thr Lys Tyr Asp Glu 
    930                 935                 940                 


Asn Asp Lys Leu Ile Arg Glu Val Lys Val Ile Thr Leu Lys Ser Lys 
945                 950                 955                 960 


Leu Val Ser Asp Phe Arg Lys Asp Phe Gln Phe Tyr Lys Val Arg Glu 
                965                 970                 975     


Ile Asn Asn Tyr His His Ala His Asp Ala Tyr Leu Asn Ala Val Val 
            980                 985                 990         


Gly Thr Ala Leu Ile Lys Lys Tyr  Pro Lys Leu Glu Ser  Glu Phe Val 
        995                 1000                 1005             


Tyr Gly  Asp Tyr Lys Val Tyr  Asp Val Arg Lys Met  Ile Ala Lys 
    1010                 1015                 1020             


Ser Glu  Gln Glu Ile Gly Lys  Ala Thr Ala Lys Tyr  Phe Phe Tyr 
    1025                 1030                 1035             


Ser Asn  Ile Met Asn Phe Phe  Lys Thr Glu Ile Thr  Leu Ala Asn 
    1040                 1045                 1050             


Gly Glu  Ile Arg Lys Arg Pro  Leu Ile Glu Thr Asn  Gly Glu Thr 
    1055                 1060                 1065             


Gly Glu  Ile Val Trp Asp Lys  Gly Arg Asp Phe Ala  Thr Val Arg 
    1070                 1075                 1080             


Lys Val  Leu Ser Met Pro Gln  Val Asn Ile Val Lys  Lys Thr Glu 
    1085                 1090                 1095             


Val Gln  Thr Gly Gly Phe Ser  Lys Glu Ser Ile Leu  Pro Lys Arg 
    1100                 1105                 1110             


Asn Ser  Asp Lys Leu Ile Ala  Arg Lys Lys Asp Trp  Asp Pro Lys 
    1115                 1120                 1125             


Lys Tyr  Gly Gly Phe Asp Ser  Pro Thr Val Ala Tyr  Ser Val Leu 
    1130                 1135                 1140             


Val Val  Ala Lys Val Glu Lys  Gly Lys Ser Lys Lys  Leu Lys Ser 
    1145                 1150                 1155             


Val Lys  Glu Leu Leu Gly Ile  Thr Ile Met Glu Arg  Ser Ser Phe 
    1160                 1165                 1170             


Glu Lys  Asn Pro Ile Asp Phe  Leu Glu Ala Lys Gly  Tyr Lys Glu 
    1175                 1180                 1185             


Val Lys  Lys Asp Leu Ile Ile  Lys Leu Pro Lys Tyr  Ser Leu Phe 
    1190                 1195                 1200             


Glu Leu  Glu Asn Gly Arg Lys  Arg Met Leu Ala Ser  Ala Gly Glu 
    1205                 1210                 1215             


Leu Gln  Lys Gly Asn Glu Leu  Ala Leu Pro Ser Lys  Tyr Val Asn 
    1220                 1225                 1230             


Phe Leu  Tyr Leu Ala Ser His  Tyr Glu Lys Leu Lys  Gly Ser Pro 
    1235                 1240                 1245             


Glu Asp  Asn Glu Gln Lys Gln  Leu Phe Val Glu Gln  His Lys His 
    1250                 1255                 1260             


Tyr Leu  Asp Glu Ile Ile Glu  Gln Ile Ser Glu Phe  Ser Lys Arg 
    1265                 1270                 1275             


Val Ile  Leu Ala Asp Ala Asn  Leu Asp Lys Val Leu  Ser Ala Tyr 
    1280                 1285                 1290             


Asn Lys  His Arg Asp Lys Pro  Ile Arg Glu Gln Ala  Glu Asn Ile 
    1295                 1300                 1305             


Ile His  Leu Phe Thr Leu Thr  Asn Leu Gly Ala Pro  Ala Ala Phe 
    1310                 1315                 1320             


Lys Tyr  Phe Asp Thr Thr Ile  Asp Arg Lys Arg Tyr  Thr Ser Thr 
    1325                 1330                 1335             


Lys Glu  Val Leu Asp Ala Thr  Leu Ile His Gln Ser  Ile Thr Gly 
    1340                 1345                 1350             


Leu Tyr  Glu Thr Arg Ile Asp  Leu Ser Gln Leu Gly  Gly Asp 
    1355                 1360                 1365         


<210>  6
<211>  4101
<212>  DNA
<213>  Artificial

<220>
<223>  Cas9 encoding

<400>  6
gacaagaagt acagcatcgg cctggacatc ggcaccaact ctgtgggctg ggccgtgatc     60

accgacgagt acaaggtgcc cagcaagaaa ttcaaggtgc tgggcaacac cgaccggcac    120

agcatcaaga agaacctgat cggagccctg ctgttcgaca gcggcgaaac agccgaggcc    180

acccggctga agagaaccgc cagaagaaga tacaccagac ggaagaaccg gatctgctat    240

ctgcaagaga tcttcagcaa cgagatggcc aaggtggacg acagcttctt ccacagactg    300

gaagagtcct tcctggtgga agaggataag aagcacgagc ggcaccccat cttcggcaac    360

atcgtggacg aggtggccta ccacgagaag taccccacca tctaccacct gagaaagaaa    420

ctggtggaca gcaccgacaa ggccgacctg cggctgatct atctggccct ggcccacatg    480

atcaagttcc ggggccactt cctgatcgag ggcgacctga accccgacaa cagcgacgtg    540

gacaagctgt tcatccagct ggtgcagacc tacaaccagc tgttcgagga aaaccccatc    600

aacgccagcg gcgtggacgc caaggccatc ctgtctgcca gactgagcaa gagcagacgg    660

ctggaaaatc tgatcgccca gctgcccggc gagaagaaga atggcctgtt cggaaacctg    720

attgccctga gcctgggcct gacccccaac ttcaagagca acttcgacct ggccgaggat    780

gccaaactgc agctgagcaa ggacacctac gacgacgacc tggacaacct gctggcccag    840

atcggcgacc agtacgccga cctgtttctg gccgccaaga acctgtccga cgccatcctg    900

ctgagcgaca tcctgagagt gaacaccgag atcaccaagg cccccctgag cgcctctatg    960

atcaagagat acgacgagca ccaccaggac ctgaccctgc tgaaagctct cgtgcggcag   1020

cagctgcctg agaagtacaa agagattttc ttcgaccaga gcaagaacgg ctacgccggc   1080

tacattgacg gcggagccag ccaggaagag ttctacaagt tcatcaagcc catcctggaa   1140

aagatggacg gcaccgagga actgctcgtg aagctgaaca gagaggacct gctgcggaag   1200

cagcggacct tcgacaacgg cagcatcccc caccagatcc acctgggaga gctgcacgcc   1260

attctgcggc ggcaggaaga tttttaccca ttcctgaagg acaaccggga aaagatcgag   1320

aagatcctga ccttccgcat cccctactac gtgggccctc tggccagggg aaacagcaga   1380

ttcgcctgga tgaccagaaa gagcgaggaa accatcaccc cctggaactt cgaggaagtg   1440

gtggacaagg gcgcttccgc ccagagcttc atcgagcgga tgaccaactt cgataagaac   1500

ctgcccaacg agaaggtgct gcccaagcac agcctgctgt acgagtactt caccgtgtat   1560

aacgagctga ccaaagtgaa atacgtgacc gagggaatga gaaagcccgc cttcctgagc   1620

ggcgagcaga aaaaggccat cgtggacctg ctgttcaaga ccaaccggaa agtgaccgtg   1680

aagcagctga aagaggacta cttcaagaaa atcgagtgct tcgactccgt ggaaatctcc   1740

ggcgtggaag atcggttcaa cgcctccctg ggcacatacc acgatctgct gaaaattatc   1800

aaggacaagg acttcctgga caatgaggaa aacgaggaca ttctggaaga tatcgtgctg   1860

accctgacac tgtttgagga cagagagatg atcgaggaac ggctgaaaac ctatgcccac   1920

ctgttcgacg acaaagtgat gaagcagctg aagcggcgga gatacaccgg ctggggcagg   1980

ctgagccgga agctgatcaa cggcatccgg gacaagcagt ccggcaagac aatcctggat   2040

ttcctgaagt ccgacggctt cgccaacaga aacttcatgc agctgatcca cgacgacagc   2100

ctgaccttta aagaggacat ccagaaagcc caggtgtccg gccagggcga tagcctgcac   2160

gagcacattg ccaatctggc cggcagcccc gccattaaga agggcatcct gcagacagtg   2220

aaggtggtgg acgagctcgt gaaagtgatg ggccggcaca agcccgagaa catcgtgatc   2280

gaaatggcca gagagaacca gaccacccag aagggacaga agaacagccg cgagagaatg   2340

aagcggatcg aagagggcat caaagagctg ggcagccaga tcctgaaaga acaccccgtg   2400

gaaaacaccc agctgcagaa cgagaagctg tacctgtact acctgcagaa tgggcgggat   2460

atgtacgtgg accaggaact ggacatcaac cggctgtccg actacgatgt ggaccatatc   2520

gtgcctcaga gctttctgaa ggacgactcc atcgacaaca aggtgctgac cagaagcgac   2580

aagaaccggg gcaagagcga caacgtgccc tccgaagagg tcgtgaagaa gatgaagaac   2640

tactggcggc agctgctgaa cgccaagctg attacccaga gaaagttcga caatctgacc   2700

aaggccgaga gaggcggcct gagcgaactg gataaggccg gcttcatcaa gagacagctg   2760

gtggaaaccc ggcagatcac aaagcacgtg gcacagatcc tggactcccg gatgaacact   2820

aagtacgacg agaatgacaa gctgatccgg gaagtgaaag tgatcaccct gaagtccaag   2880

ctggtgtccg atttccggaa ggatttccag ttttacaaag tgcgcgagat caacaactac   2940

caccacgccc acgacgccta cctgaacgcc gtcgtgggaa ccgccctgat caaaaagtac   3000

cctaagctgg aaagcgagtt cgtgtacggc gactacaagg tgtacgacgt gcggaagatg   3060

atcgccaaga gcgagcagga aatcggcaag gctaccgcca agtacttctt ctacagcaac   3120

atcatgaact ttttcaagac cgagattacc ctggccaacg gcgagatccg gaagcggcct   3180

ctgatcgaga caaacggcga aaccggggag atcgtgtggg ataagggccg ggattttgcc   3240

accgtgcgga aagtgctgag catgccccaa gtgaatatcg tgaaaaagac cgaggtgcag   3300

acaggcggct tcagcaaaga gtctatcctg cccaagagga acagcgataa gctgatcgcc   3360

agaaagaagg actgggaccc taagaagtac ggcggcttcg acagccccac cgtggcctat   3420

tctgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa gagtgtgaaa   3480

gagctgctgg ggatcaccat catggaaaga agcagcttcg agaagaatcc catcgacttt   3540

ctggaagcca agggctacaa agaagtgaaa aaggacctga tcatcaagct gcctaagtac   3600

tccctgttcg agctggaaaa cggccggaag agaatgctgg cctctgccgg cgaactgcag   3660

aagggaaacg aactggccct gccctccaaa tatgtgaact tcctgtacct ggccagccac   3720

tatgagaagc tgaagggctc ccccgaggat aatgagcaga aacagctgtt tgtggaacag   3780

cacaagcact acctggacga gatcatcgag cagatcagcg agttctccaa gagagtgatc   3840

ctggccgacg ctaatctgga caaagtgctg tccgcctaca acaagcaccg ggataagccc   3900

atcagagagc aggccgagaa tatcatccac ctgtttaccc tgaccaatct gggagcccct   3960

gccgccttca agtactttga caccaccatc gaccggaaga ggtacaccag caccaaagag   4020

gtgctggacg ccaccctgat ccaccagagc atcaccggcc tgtacgagac acggatcgac   4080

ctgtctcagc tgggaggcga c                                             4101


<210>  7
<211>  8
<212>  PRT
<213>  Listeria monocytogenes


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  site of receptor domain insertion

<400>  7

Gly Trp Asn Gln Glu Tyr Glu Asp 
1               5               


<210>  8
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #2


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  site of receptor domain insertion

<400>  8

Gly Trp Asn Gln Glu Gly Gly Tyr Glu Asp 
1               5                   10  


<210>  9
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #3


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  site of receptor domain insertion

<400>  9

Gly Trp Asn Gln Glu Ser Gly Gly Ser Tyr Glu Asp 
1               5                   10          


<210>  10
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #4


<220>
<221>  MISC_FEATURE
<222>  (4)..(5)
<223>  site of receptor domain insertion

<400>  10

Gly Trp Asn Gln Tyr Glu Asp 
1               5           


<210>  11
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #5


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  site of receptor domain insertion

<400>  11

Gly Trp Asn Gln Gly Gly Tyr Glu Asp 
1               5                   


<210>  12
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #6


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  site of receptor domain insertion

<400>  12

Gly Trp Asn Gln Ser Gly Gly Ser Tyr Glu Asp 
1               5                   10      


<210>  13
<211>  6
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #7


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  site of receptor domain insertion

<400>  13

Gly Trp Asn Tyr Glu Asp 
1               5       


<210>  14
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #8


<220>
<221>  MISC_FEATURE
<222>  (4)..(5)
<223>  site of receptor domain insertion

<400>  14

Gly Trp Asn Gly Gly Tyr Glu Asp 
1               5               


<210>  15
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #9


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  site of receptor domain insertion

<400>  15

Gly Trp Asn Ser Gly Gly Ser Tyr Glu Asp 
1               5                   10  


<210>  16
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #10


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  site of receptor domain insertion

<400>  16

Gly Trp Asn Ser Gly Gly Gly Gly Ser Tyr Glu Asp 
1               5                   10          


<210>  17
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #11


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  site of receptor domain insertion

<400>  17

Gly Trp Asn Gly Ser Gly Gly Ser Gly Tyr Glu Asp 
1               5                   10          


<210>  18
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #12


<220>
<221>  MISC_FEATURE
<222>  (10)..(11)
<223>  site of receptor domain insertion

<400>  18

Gly Trp Asn Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Gly Tyr Glu Asp 
            20  


<210>  19
<211>  21
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #13


<220>
<221>  MISC_FEATURE
<222>  (11)..(12)
<223>  site of receptor domain insertion

<400>  19

Gly Trp Asn Gln Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
1               5                   10                  15      


Gly Gly Tyr Glu Asp 
            20      


<210>  20
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #Rap4


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  site of receptor domain insertion

<400>  20

Gly Trp Asn Ser Gly Gly Ser Tyr Glu Asp 
1               5                   10  


<210>  21
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #Rap5


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  site of receptor domain insertion

<400>  21

Gly Trp Asn Gln Ser Gly Gly Ser Tyr Glu Asp 
1               5                   10      


<210>  22
<211>  14
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #Rap6


<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  site of receptor domain insertion

<400>  22

Gly Trp Asn Gln Glu Gly Ser Gly Gly Ser Gly Tyr Glu Asp 
1               5                   10                  


<210>  23
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  N-terminal fusion including amino acids 2 to 13 of ACRIIA4, 
       variant #8


<220>
<221>  HELIX
<222>  (1)..(9)
<223>  LOV2

<220>
<221>  PEPTIDE
<222>  (10)..(22)
<223>  AcrIIA4 aa2 to 14

<400>  23

Asn Ile Asp Glu Ala Ala Lys Glu Leu Asn Ile Asn Asp Leu Ile Arg 
1               5                   10                  15      


Glu Ile Lys Asn Lys Asp 
            20          


<210>  24
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  N-terminal fusion including amino acids 2 to 13 of ACRIIA4, 
       variant #41


<220>
<221>  HELIX
<222>  (1)..(9)
<223>  LOV2

<220>
<221>  PEPTIDE
<222>  (10)..(22)
<223>  AcrIIA4 aa2 to 14

<400>  24

Asn Ile Asp Glu Leu Ala Lys Ala Leu Asn Ile Asn Asp Leu Ile Arg 
1               5                   10                  15      


Glu Ile Lys Asn Lys Asp 
            20          


<210>  25
<211>  22
<212>  PRT
<213>  Artificial

<220>
<223>  N-terminal fusion including amino acids 2 to 13 of ACRIIA4, 
       variant #51


<220>
<221>  HELIX
<222>  (1)..(9)
<223>  LOV2

<220>
<221>  HELIX
<222>  (10)..(22)
<223>  AcrIIA4 aa2 to 14

<400>  25

Asn Ile Glu Glu Leu Leu Lys Arg Leu Asn Ile Asn Asp Leu Ile Arg 
1               5                   10                  15      


Glu Ile Lys Asn Lys Asp 
            20          


<210>  26
<211>  20
<212>  PRT
<213>  Artificial

<220>
<223>  N-terminal fusion including amino acids 4 to 13 of ACRIIA4, 
       variant #53-41


<220>
<221>  HELIX
<222>  (1)..(9)
<223>  LOV2

<220>
<221>  PEPTIDE
<222>  (10)..(20)

<400>  26

Asn Ile Asp Glu Leu Ala Lys Ala Leu Asn Asp Leu Ile Arg Glu Ile 
1               5                   10                  15      


Lys Asn Lys Asp 
            20  


<210>  27
<211>  23
<212>  PRT
<213>  Artificial

<220>
<223>  N-terminal fusion including amino acids 2 to 13 of ACRIIA4, 
       variant #69-41


<220>
<221>  HELIX
<222>  (1)..(9)
<223>  LOV2

<220>
<221>  MISC_FEATURE
<222>  (10)..(10)
<223>  Linker

<220>
<221>  PEPTIDE
<222>  (11)..(23)
<223>  AcrIIA4 aa2 to 14

<400>  27

Asn Ile Asp Glu Leu Ala Lys Ala Leu Ala Asn Ile Asn Asp Leu Ile 
1               5                   10                  15      


Arg Glu Ile Lys Asn Lys Asp 
            20              


<210>  28
<211>  24
<212>  PRT
<213>  Artificial

<220>
<223>  N-terminal fusion including amino acids 2 to 13 of ACRIIA4, 
       variant #71-51


<220>
<221>  HELIX
<222>  (1)..(9)
<223>  LOV2

<220>
<221>  MISC_FEATURE
<222>  (10)..(11)
<223>  Linker

<220>
<221>  PEPTIDE
<222>  (12)..(24)
<223>  AcrIIA4 aa2 to 14

<400>  28

Asn Ile Glu Glu Leu Leu Lys Arg Leu Gly Pro Asn Ile Asn Asp Leu 
1               5                   10                  15      


Ile Arg Glu Ile Lys Asn Lys Asp 
            20                  


<210>  29
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  CFTR guideRNA

<400>  29
gaatggtgcc aggcataatc c                                               21


<210>  30
<211>  21
<212>  DNA
<213>  Artificial

<220>
<223>  CFTR_fw primer

<400>  30
gcacatagaa cagcactcga c                                               21


<210>  31
<211>  23
<212>  DNA
<213>  Artificial

<220>
<223>  CFTR_re primer

<400>  31
gatccattca cagtagctta ccc                                             23


<210>  32
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  GGSGGSG linker

<400>  32

Gly Gly Ser Gly Gly Ser Gly 
1               5           


<210>  33
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  GSGGSGG linker

<400>  33

Gly Ser Gly Gly Ser Gly Gly 
1               5           


<210>  34
<211>  143
<212>  PRT
<213>  Avena sativa

<400>  34

Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp 
1               5                   10                  15      


Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu 
            20                  25                  30          


Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg 
        35                  40                  45              


Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg 
    50                  55                  60                  


Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr 
65                  70                  75                  80  


Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met 
                85                  90                  95      


Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp 
            100                 105                 110         


Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu 
        115                 120                 125             


Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu 
    130                 135                 140             


<210>  35
<211>  429
<212>  DNA
<213>  Avena sativa

<400>  35
cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct     60

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    120

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    180

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    240

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    300

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    360

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    420

aaagagttg                                                            429


<210>  36
<211>  198
<212>  PRT
<213>  Artificial

<220>
<223>  UniRapB domain

<400>  36

Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Phe 
1               5                   10                  15      


Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys 
            20                  25                  30          


Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val 
        35                  40                  45              


Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala 
    50                  55                  60                  


Thr Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys Asp Leu Leu Gln 
65                  70                  75                  80  


Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Pro Pro 
                85                  90                  95      


Gly Pro Gly Ser Gly Leu Trp His Glu Met Trp His Glu Gly Leu Glu 
            100                 105                 110         


Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe 
        115                 120                 125             


Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr 
    130                 135                 140                 


Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu 
145                 150                 155                 160 


Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Ser Ser Gly Gly 
                165                 170                 175     


Ser Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val 
            180                 185                 190         


Glu Leu Leu Lys Leu Glu 
        195             


<210>  37
<211>  594
<212>  DNA
<213>  Artificial

<220>
<223>  UniRapB domain encoding sequence

<400>  37
acctgcgtgg tgcattacac gggaatgctc gaggacggca aaaagttcga ttctagtcgc     60

gacagaaata agccttttaa gtttatgctt ggaaagcaag aagtcatccg cgggtgggag    120

gaaggcgttg ctcaaatgtc tgtaggtcaa cgagctaaac ttaccatttc tccagattac    180

gcatacggtg cgacggggca cggatctggc tcaggatctg gtgtaaagga tctgctccaa    240

gcctgggatc tttattatca tgtgtttcgc cgaatcagtg gccctccggg cccaggaagc    300

ggactgtggc atgaaatgtg gcacgagggt cttgaggagg cgagtagact gtatttcggt    360

gagagaaatg ttaaggggat gttcgaggtg ctggagccgc tgcacgccat gatggaaagg    420

ggcccacaaa cgcttaaaga aaccagcttc aatcaggcat acggcaggga tttgatggag    480

gctcaggaat ggtgtcgaaa atacatgaaa tcaggtagct caggtgggag cgggagtggg    540

ataattcctc cccacgcgac tctcgtgttc gacgtagaat tgcttaaact ggaa          594


<210>  38
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between G62/W63, encoding

<400>  38
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ccttgctaca    240

actttggagc ggattgagaa gaatttcgtc ataactgatc ccaggttgcc tgataatcct    300

ataatattcg cctccgatag ttttcttcaa cttacggagt actccagaga ggagatactg    360

ggaaggaatt gcagattcct ccaaggtcca gaaactgata gagctactgt gaggaagata    420

cgggatgcca ttgacaatca aactgaagtg acagtgcaac ttatcaatta caccaagtca    480

gggaaaaaat tctggaacct gttccatttg caacccatga gggaccaaaa gggcgatgtg    540

caatatttca taggcgttca acttgatggt actgaacacg tcagagatgc ggcggagcga    600

gaaggagtga tgctgattaa gaaaacagca gagaatatcg acgaagcggc gaaagagttg    660

tggaaccagg agtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  39
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between W63/N64, encoding

<400>  39
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgaaccagg agtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  40
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between N64/Q65, encoding

<400>  40
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacctt    240

gctacaactt tggagcggat tgagaagaat ttcgtcataa ctgatcccag gttgcctgat    300

aatcctataa tattcgcctc cgatagtttt cttcaactta cggagtactc cagagaggag    360

atactgggaa ggaattgcag attcctccaa ggtccagaaa ctgatagagc tactgtgagg    420

aagatacggg atgccattga caatcaaact gaagtgacag tgcaacttat caattacacc    480

aagtcaggga aaaaattctg gaacctgttc catttgcaac ccatgaggga ccaaaagggc    540

gatgtgcaat atttcatagg cgttcaactt gatggtactg aacacgtcag agatgcggcg    600

gagcgagaag gagtgatgct gattaagaaa acagcagaga atatcgacga agcggcgaaa    660

gagttgcagg agtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  41
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between Q65/E66, encoding

<400>  41
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    300

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    360

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    420

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    480

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    540

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    600

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    660

aaagagttgg agtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  42
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between E66/Y67, encoding

<400>  42
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagcttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    300

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    360

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    420

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    480

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    540

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    600

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    660

gcgaaagagt tgtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  43
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between Y67/E68, encoding

<400>  43
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagtaccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttggaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  44
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between E68/D69, encoding

<400>  44
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagtacgaac ttgctacaac tttggagcgg attgagaaga atttcgtcat aactgatccc    300

aggttgcctg ataatcctat aatattcgcc tccgatagtt ttcttcaact tacggagtac    360

tccagagagg agatactggg aaggaattgc agattcctcc aaggtccaga aactgataga    420

gctactgtga ggaagatacg ggatgccatt gacaatcaaa ctgaagtgac agtgcaactt    480

atcaattaca ccaagtcagg gaaaaaattc tggaacctgt tccatttgca acccatgagg    540

gaccaaaagg gcgatgtgca atatttcata ggcgttcaac ttgatggtac tgaacacgtc    600

agagatgcgg cggagcgaga aggagtgatg ctgattaaga aaacagcaga gaatatcgac    660

gaagcggcga aagagttgga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  45
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between E85/L86, encoding

<400>  45
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    300

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    360

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    420

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    480

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    540

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    600

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    660

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    720

aaagagttgc tgaactag                                                  738


<210>  46
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between L86/N87, encoding

<400>  46
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    300

ctgcttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    360

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    420

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    480

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    540

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    600

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    660

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    720

gcgaaagagt tgaactag                                                  738


<210>  47
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion after N87, encoding

<400>  47
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    300

ctgaaccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    360

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    420

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    480

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    540

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    600

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    660

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    720

gcggcgaaag agttgtag                                                  738


<210>  48
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQE[LOV2]YED - (#1), encoding

<400>  48
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagcttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    300

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    360

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    420

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    480

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    540

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    600

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    660

gcgaaagagt tgtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  49
<211>  744
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQEG[LOV2]GYED - (#2), encoding

<400>  49
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcta cgaagatgag gaagagttct acaatgatat gcaaactatc    720

acactgaaat ctgagctgaa ctag                                           744


<210>  50
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQESG[LOV2]GSYED - (#3), encoding

<400>  50
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagagcggcc ttgctacaac tttggagcgg attgagaaga atttcgtcat aactgatccc    300

aggttgcctg ataatcctat aatattcgcc tccgatagtt ttcttcaact tacggagtac    360

tccagagagg agatactggg aaggaattgc agattcctcc aaggtccaga aactgataga    420

gctactgtga ggaagatacg ggatgccatt gacaatcaaa ctgaagtgac agtgcaactt    480

atcaattaca ccaagtcagg gaaaaaattc tggaacctgt tccatttgca acccatgagg    540

gaccaaaagg gcgatgtgca atatttcata ggcgttcaac ttgatggtac tgaacacgtc    600

agagatgcgg cggagcgaga aggagtgatg ctgattaaga aaacagcaga gaatatcgac    660

gaagcggcga aagagttggg cagctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  51
<211>  735
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQ[LOV2]YED - (#4), encoding

<400>  51
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    300

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    360

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    420

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    480

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    540

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    600

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    660

aaagagttgt acgaagatga ggaagagttc tacaatgata tgcaaactat cacactgaaa    720

tctgagctga actag                                                     735


<210>  52
<211>  741
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQG[LOV2]GYED - (#5), encoding

<400>  52
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

ggccttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    300

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    360

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    420

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    480

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    540

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    600

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    660

gcgaaagagt tgggctacga agatgaggaa gagttctaca atgatatgca aactatcaca    720

ctgaaatctg agctgaacta g                                              741


<210>  53
<211>  747
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQSG[LOV2]GSYED - (#6), encoding

<400>  53
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag ctacgaagat gaggaagagt tctacaatga tatgcaaact    720

atcacactga aatctgagct gaactag                                        747


<210>  54
<211>  732
<212>  DNA
<213>  Artificial

<220>
<223>  GWN[LOV2]YED  - (#7), encoding

<400>  54
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacctt    240

gctacaactt tggagcggat tgagaagaat ttcgtcataa ctgatcccag gttgcctgat    300

aatcctataa tattcgcctc cgatagtttt cttcaactta cggagtactc cagagaggag    360

atactgggaa ggaattgcag attcctccaa ggtccagaaa ctgatagagc tactgtgagg    420

aagatacggg atgccattga caatcaaact gaagtgacag tgcaacttat caattacacc    480

aagtcaggga aaaaattctg gaacctgttc catttgcaac ccatgaggga ccaaaagggc    540

gatgtgcaat atttcatagg cgttcaactt gatggtactg aacacgtcag agatgcggcg    600

gagcgagaag gagtgatgct gattaagaaa acagcagaga atatcgacga agcggcgaaa    660

gagttgtacg aagatgagga agagttctac aatgatatgc aaactatcac actgaaatct    720

gagctgaact ag                                                        732


<210>  55
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  GWNG[LOV2]GYED - (#8), encoding

<400>  55
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    300

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    360

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    420

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    480

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    540

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    600

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    660

aaagagttgg gctacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  56
<211>  744
<212>  DNA
<213>  Artificial

<220>
<223>  GWNSG[LOV2]GSYED - (#9), encoding

<400>  56
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacagc    240

ggccttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    300

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    360

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    420

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    480

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    540

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    600

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    660

gcgaaagagt tgggcagcta cgaagatgag gaagagttct acaatgatat gcaaactatc    720

acactgaaat ctgagctgaa ctag                                           744


<210>  57
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  GWNSGG[LOV2]GGSYED - (#10), encoding

<400>  57
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacagc    240

ggcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcgg cagctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  58
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  GWNGSG[LOV2]GSGYED - (#11), encoding

<400>  58
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  59
<211>  774
<212>  DNA
<213>  Artificial

<220>
<223>  GWNGGSGGSG[LOV2]GSGGSGGYED - (#12), encoding

<400>  59
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

ggcagcggcg gcagcggcct tgctacaact ttggagcgga ttgagaagaa tttcgtcata    300

actgatccca ggttgcctga taatcctata atattcgcct ccgatagttt tcttcaactt    360

acggagtact ccagagagga gatactggga aggaattgca gattcctcca aggtccagaa    420

actgatagag ctactgtgag gaagatacgg gatgccattg acaatcaaac tgaagtgaca    480

gtgcaactta tcaattacac caagtcaggg aaaaaattct ggaacctgtt ccatttgcaa    540

cccatgaggg accaaaaggg cgatgtgcaa tatttcatag gcgttcaact tgatggtact    600

gaacacgtca gagatgcggc ggagcgagaa ggagtgatgc tgattaagaa aacagcagag    660

aatatcgacg aagcggcgaa agagttgggc agcggcggca gcggcggcta cgaagatgag    720

gaagagttct acaatgatat gcaaactatc acactgaaat ctgagctgaa ctag          774


<210>  60
<211>  777
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQGGSGGSG[LOV2]GSGGSGGYED - (#13), encoding

<400>  60
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

ggcggcagcg gcggcagcgg ccttgctaca actttggagc ggattgagaa gaatttcgtc    300

ataactgatc ccaggttgcc tgataatcct ataatattcg cctccgatag ttttcttcaa    360

cttacggagt actccagaga ggagatactg ggaaggaatt gcagattcct ccaaggtcca    420

gaaactgata gagctactgt gaggaagata cgggatgcca ttgacaatca aactgaagtg    480

acagtgcaac ttatcaatta caccaagtca gggaaaaaat tctggaacct gttccatttg    540

caacccatga gggaccaaaa gggcgatgtg caatatttca taggcgttca acttgatggt    600

actgaacacg tcagagatgc ggcggagcga gaaggagtga tgctgattaa gaaaacagca    660

gagaatatcg acgaagcggc gaaagagttg ggcagcggcg gcagcggcgg ctacgaagat    720

gaggaagagt tctacaatga tatgcaaact atcacactga aatctgagct gaactag       777


<210>  61
<211>  903
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQE[UniRap]YED - (#Rap1), encoding

<400>  61
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagacctgcg tggtgcatta cacgggaatg ctcgaggacg gcaaaaagtt cgattctagt    300

cgcgacagaa ataagccttt taagtttatg cttggaaagc aagaagtcat ccgcgggtgg    360

gaggaaggcg ttgctcaaat gtctgtaggt caacgagcta aacttaccat ttctccagat    420

tacgcatacg gtgcgacggg gcacggatct ggctcaggat ctggtgtaaa ggatctgctc    480

caagcctggg atctttatta tcatgtgttt cgccgaatca gtggccctcc gggcccagga    540

agcggactgt ggcatgaaat gtggcacgag ggtcttgagg aggcgagtag actgtatttc    600

ggtgagagaa atgttaaggg gatgttcgag gtgctggagc cgctgcacgc catgatggaa    660

aggggcccac aaacgcttaa agaaaccagc ttcaatcagg catacggcag ggatttgatg    720

gaggctcagg aatggtgtcg aaaatacatg aaatcaggta gctcaggtgg gagcgggagt    780

gggataattc ctccccacgc gactctcgtg ttcgacgtag aattgcttaa actggaatac    840

gaagatgagg aagagttcta caatgatatg caaactatca cactgaaatc tgagctgaac    900

tag                                                                  903


<210>  62
<211>  900
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQ[UniRap]YED - (#Rap2), encoding

<400>  62
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

acctgcgtgg tgcattacac gggaatgctc gaggacggca aaaagttcga ttctagtcgc    300

gacagaaata agccttttaa gtttatgctt ggaaagcaag aagtcatccg cgggtgggag    360

gaaggcgttg ctcaaatgtc tgtaggtcaa cgagctaaac ttaccatttc tccagattac    420

gcatacggtg cgacggggca cggatctggc tcaggatctg gtgtaaagga tctgctccaa    480

gcctgggatc tttattatca tgtgtttcgc cgaatcagtg gccctccggg cccaggaagc    540

ggactgtggc atgaaatgtg gcacgagggt cttgaggagg cgagtagact gtatttcggt    600

gagagaaatg ttaaggggat gttcgaggtg ctggagccgc tgcacgccat gatggaaagg    660

ggcccacaaa cgcttaaaga aaccagcttc aatcaggcat acggcaggga tttgatggag    720

gctcaggaat ggtgtcgaaa atacatgaaa tcaggtagct caggtgggag cgggagtggg    780

ataattcctc cccacgcgac tctcgtgttc gacgtagaat tgcttaaact ggaatacgaa    840

gatgaggaag agttctacaa tgatatgcaa actatcacac tgaaatctga gctgaactag    900


<210>  63
<211>  897
<212>  DNA
<213>  Artificial

<220>
<223>  GWN[UniRap]YED - (#Rap3), encoding

<400>  63
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacacc    240

tgcgtggtgc attacacggg aatgctcgag gacggcaaaa agttcgattc tagtcgcgac    300

agaaataagc cttttaagtt tatgcttgga aagcaagaag tcatccgcgg gtgggaggaa    360

ggcgttgctc aaatgtctgt aggtcaacga gctaaactta ccatttctcc agattacgca    420

tacggtgcga cggggcacgg atctggctca ggatctggtg taaaggatct gctccaagcc    480

tgggatcttt attatcatgt gtttcgccga atcagtggcc ctccgggccc aggaagcgga    540

ctgtggcatg aaatgtggca cgagggtctt gaggaggcga gtagactgta tttcggtgag    600

agaaatgtta aggggatgtt cgaggtgctg gagccgctgc acgccatgat ggaaaggggc    660

ccacaaacgc ttaaagaaac cagcttcaat caggcatacg gcagggattt gatggaggct    720

caggaatggt gtcgaaaata catgaaatca ggtagctcag gtgggagcgg gagtgggata    780

attcctcccc acgcgactct cgtgttcgac gtagaattgc ttaaactgga atacgaagat    840

gaggaagagt tctacaatga tatgcaaact atcacactga aatctgagct gaactag       897


<210>  64
<211>  909
<212>  DNA
<213>  Artificial

<220>
<223>  GWNSG[UniRap]GSYED - (#Rap4), encoding

<400>  64
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacagc    240

ggcacctgcg tggtgcatta cacgggaatg ctcgaggacg gcaaaaagtt cgattctagt    300

cgcgacagaa ataagccttt taagtttatg cttggaaagc aagaagtcat ccgcgggtgg    360

gaggaaggcg ttgctcaaat gtctgtaggt caacgagcta aacttaccat ttctccagat    420

tacgcatacg gtgcgacggg gcacggatct ggctcaggat ctggtgtaaa ggatctgctc    480

caagcctggg atctttatta tcatgtgttt cgccgaatca gtggccctcc gggcccagga    540

agcggactgt ggcatgaaat gtggcacgag ggtcttgagg aggcgagtag actgtatttc    600

ggtgagagaa atgttaaggg gatgttcgag gtgctggagc cgctgcacgc catgatggaa    660

aggggcccac aaacgcttaa agaaaccagc ttcaatcagg catacggcag ggatttgatg    720

gaggctcagg aatggtgtcg aaaatacatg aaatcaggta gctcaggtgg gagcgggagt    780

gggataattc ctccccacgc gactctcgtg ttcgacgtag aattgcttaa actggaaggc    840

agctacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    900

ctgaactag                                                            909


<210>  65
<211>  912
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQSG[UniRap]GSYED - (#Rap5), encoding

<400>  65
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

agcggcacct gcgtggtgca ttacacggga atgctcgagg acggcaaaaa gttcgattct    300

agtcgcgaca gaaataagcc ttttaagttt atgcttggaa agcaagaagt catccgcggg    360

tgggaggaag gcgttgctca aatgtctgta ggtcaacgag ctaaacttac catttctcca    420

gattacgcat acggtgcgac ggggcacgga tctggctcag gatctggtgt aaaggatctg    480

ctccaagcct gggatcttta ttatcatgtg tttcgccgaa tcagtggccc tccgggccca    540

ggaagcggac tgtggcatga aatgtggcac gagggtcttg aggaggcgag tagactgtat    600

ttcggtgaga gaaatgttaa ggggatgttc gaggtgctgg agccgctgca cgccatgatg    660

gaaaggggcc cacaaacgct taaagaaacc agcttcaatc aggcatacgg cagggatttg    720

atggaggctc aggaatggtg tcgaaaatac atgaaatcag gtagctcagg tgggagcggg    780

agtgggataa ttcctcccca cgcgactctc gtgttcgacg tagaattgct taaactggaa    840

ggcagctacg aagatgagga agagttctac aatgatatgc aaactatcac actgaaatct    900

gagctgaact ag                                                        912


<210>  66
<211>  921
<212>  DNA
<213>  Artificial

<220>
<223>  GWNQEGSG[UniRap]GSGYED - (#Rap6), encoding

<400>  66
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagggcagcg gcacctgcgt ggtgcattac acgggaatgc tcgaggacgg caaaaagttc    300

gattctagtc gcgacagaaa taagcctttt aagtttatgc ttggaaagca agaagtcatc    360

cgcgggtggg aggaaggcgt tgctcaaatg tctgtaggtc aacgagctaa acttaccatt    420

tctccagatt acgcatacgg tgcgacgggg cacggatctg gctcaggatc tggtgtaaag    480

gatctgctcc aagcctggga tctttattat catgtgtttc gccgaatcag tggccctccg    540

ggcccaggaa gcggactgtg gcatgaaatg tggcacgagg gtcttgagga ggcgagtaga    600

ctgtatttcg gtgagagaaa tgttaagggg atgttcgagg tgctggagcc gctgcacgcc    660

atgatggaaa ggggcccaca aacgcttaaa gaaaccagct tcaatcaggc atacggcagg    720

gatttgatgg aggctcagga atggtgtcga aaatacatga aatcaggtag ctcaggtggg    780

agcgggagtg ggataattcc tccccacgcg actctcgtgt tcgacgtaga attgcttaaa    840

ctggaaggca gcggctacga agatgaggaa gagttctaca atgatatgca aactatcaca    900

ctgaaatctg agctgaacta g                                              921


<210>  67
<211>  915
<212>  DNA
<213>  Artificial

<220>
<223>  GWNGSG[UniRap]GSGYED - (#Rap7), encoding

<400>  67
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggcacct gcgtggtgca ttacacggga atgctcgagg acggcaaaaa gttcgattct    300

agtcgcgaca gaaataagcc ttttaagttt atgcttggaa agcaagaagt catccgcggg    360

tgggaggaag gcgttgctca aatgtctgta ggtcaacgag ctaaacttac catttctcca    420

gattacgcat acggtgcgac ggggcacgga tctggctcag gatctggtgt aaaggatctg    480

ctccaagcct gggatcttta ttatcatgtg tttcgccgaa tcagtggccc tccgggccca    540

ggaagcggac tgtggcatga aatgtggcac gagggtcttg aggaggcgag tagactgtat    600

ttcggtgaga gaaatgttaa ggggatgttc gaggtgctgg agccgctgca cgccatgatg    660

gaaaggggcc cacaaacgct taaagaaacc agcttcaatc aggcatacgg cagggatttg    720

atggaggctc aggaatggtg tcgaaaatac atgaaatcag gtagctcagg tgggagcggg    780

agtgggataa ttcctcccca cgcgactctc gtgttcgacg tagaattgct taaactggaa    840

ggcagcggct acgaagatga ggaagagttc tacaatgata tgcaaactat cacactgaaa    900

tctgagctga actag                                                     915


<210>  68
<211>  1452
<212>  DNA
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4 (#8), encoding

<400>  68
atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc     60

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag    120

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg    180

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg    240

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc    300

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc    360

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc    420

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc    480

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag    540

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc    600

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag    660

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg    720

gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtttggctac tacacttgaa    780

cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc    840

gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac    900

tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc    960

atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag   1020

ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt   1080

attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc   1140

atgctgatta agaaaactgc agaaaatatt gacgaagcgg cgaaggagtt gaacattaac   1200

gacctgattc gggaaataaa gaacaaggac tacacggtca agctgtcagg caccgactcc   1260

aactctataa cacagctcat tataagggtt aataatgatg gcaacgaata tgtcatctct   1320

gaatccgaga atgaatcaat cgtggagaaa ttcatcagtg catttaaaaa cggctggaac   1380

caggagtacg aagatgagga agagttctac aatgatatgc aaactatcac actgaaatct   1440

gagctgaact ag                                                       1452


<210>  69
<211>  1452
<212>  DNA
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4A(#41), encoding

<400>  69
atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc     60

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag    120

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg    180

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg    240

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc    300

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc    360

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc    420

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc    480

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag    540

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc    600

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag    660

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg    720

gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtttggctac tacacttgaa    780

cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc    840

gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac    900

tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc    960

atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag   1020

ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt   1080

attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc   1140

atgctgatta agaaaactgc agaaaatatt gatgaactgg ctaaagcact taatataaat   1200

gacctgataa gggaaataaa gaataaggac tacacggtca agctgtcagg caccgactcc   1260

aactctataa cacagctcat tataagggtt aataatgatg gcaacgaata tgtcatctct   1320

gaatccgaga atgaatcaat cgtggagaaa ttcatcagtg catttaaaaa cggctggaac   1380

caggagtacg aagatgagga agagttctac aatgatatgc aaactatcac actgaaatct   1440

gagctgaact ag                                                       1452


<210>  70
<211>  1452
<212>  DNA
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4  (#51), encoding

<400>  70
atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc     60

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag    120

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg    180

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg    240

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc    300

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc    360

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc    420

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc    480

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag    540

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc    600

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag    660

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg    720

gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtttggctac tacacttgaa    780

cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc    840

gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac    900

tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc    960

atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag   1020

ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt   1080

attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc   1140

atgctgatta agaaaactgc agaaaatatt gaagaactcc tgaagagact taacataaac   1200

gatctcataa gagagataaa gaataaagac tacacggtca agctgtcagg caccgactcc   1260

aactctataa cacagctcat tataagggtt aataatgatg gcaacgaata tgtcatctct   1320

gaatccgaga atgaatcaat cgtggagaaa ttcatcagtg catttaaaaa cggctggaac   1380

caggagtacg aagatgagga agagttctac aatgatatgc aaactatcac actgaaatct   1440

gagctgaact ag                                                       1452


<210>  71
<211>  1446
<212>  DNA
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4(#53-41), encoding

<400>  71
atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc     60

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag    120

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg    180

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg    240

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc    300

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc    360

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc    420

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc    480

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag    540

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc    600

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag    660

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg    720

gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtttggctac tacacttgaa    780

cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc    840

gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac    900

tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc    960

atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag   1020

ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt   1080

attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc   1140

atgctgatta agaaaactgc agaaaatatt gatgaactgg ctaaagcact taacgacctc   1200

atccgcgaga tcaagaacaa ggactacacg gtcaagctgt caggcaccga ctccaactct   1260

ataacacagc tcattataag ggttaataat gatggcaacg aatatgtcat ctctgaatcc   1320

gagaatgaat caatcgtgga gaaattcatc agtgcattta aaaacggctg gaaccaggag   1380

tacgaagatg aggaagagtt ctacaatgat atgcaaacta tcacactgaa atctgagctg   1440

aactag                                                              1446


<210>  72
<211>  1455
<212>  DNA
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4A  (#69-41), encoding

<400>  72
atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc     60

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag    120

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg    180

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg    240

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc    300

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc    360

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc    420

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc    480

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag    540

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc    600

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag    660

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg    720

gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtttggctac tacacttgaa    780

cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc    840

gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac    900

tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc    960

atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag   1020

ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt   1080

attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc   1140

atgctgatta agaaaactgc agaaaatatt gatgaactgg ctaaagcact tgccaacatt   1200

aacgacctca tccgcgagat caagaacaag gactacacgg tcaagctgtc aggcaccgac   1260

tccaactcta taacacagct cattataagg gttaataatg atggcaacga atatgtcatc   1320

tctgaatccg agaatgaatc aatcgtggag aaattcatca gtgcatttaa aaacggctgg   1380

aaccaggagt acgaagatga ggaagagttc tacaatgata tgcaaactat cacactgaaa   1440

tctgagctga actag                                                    1455


<210>  73
<211>  1458
<212>  DNA
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4A  (#71-51), encoding

<400>  73
atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc     60

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag    120

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg    180

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg    240

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc    300

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc    360

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc    420

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc    480

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag    540

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc    600

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag    660

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg    720

gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtttggctac tacacttgaa    780

cgtattgaga agaactttgt cattactgac ccaagattgc cagataatcc cattatattc    840

gcgtccgata gtttcttgca gttgacagaa tatagccgtg aagaaatttt gggaagaaac    900

tgcaggtttc tacaaggtcc tgaaactgat cgcgcgacag tgagaaaaat tagagatgcc    960

atagataacc aaacagaggt cactgttcag ctgattaatt atacaaagag tggtaaaaag   1020

ttctggaacc tctttcactt gcagcctatg cgagatcaga agggagatgt ccagtacttt   1080

attggggttc agttggatgg aactgagcat gtccgagatg ctgccgagag agagggagtc   1140

atgctgatta agaaaactgc agaaaatatt gaagaactcc tgaagagact tgggcctaac   1200

atcaatgatt tgattagaga aattaagaac aaggactaca cggtcaagct gtcaggcacc   1260

gactccaact ctataacaca gctcattata agggttaata atgatggcaa cgaatatgtc   1320

atctctgaat ccgagaatga atcaatcgtg gagaaattca tcagtgcatt taaaaacggc   1380

tggaaccagg agtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg   1440

aaatctgagc tgaactag                                                 1458


<210>  74
<211>  1500
<212>  DNA
<213>  Artificial

<220>
<223>  cMyc NLS-AcrIIA4-mCherry-LEXY (pl76), encoding

<400>  74
atgcccgcag caaagcgcgt gaagctggac atgggtggat caaacattaa cgacctgatt     60

cgggaaataa agaacaagga ctacacggtc aagctgtcag gcaccgactc caactctata    120

acacagctca ttataagggt taataatgat ggcaacgaat atgtcatctc tgaatccgag    180

aatgaatcaa tcgtggagaa attcatcagt gcatttaaaa acggctggaa ccaggagtac    240

gaagatgagg aagagttcta caatgatatg caaactatca cactgaaatc tgagctgaac    300

ggcgggtctg gtggttctgt gagcaagggc gaggaggata acatggccat catcaaggag    360

ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag    420

ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag    480

ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag    540

gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc    600

ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac    660

tcctccctgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc    720

tccgacggcc ccgtaatgca gaagaaaacc atgggctggg aggcctcctc cgagcggatg    780

taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc    840

ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc    900

ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc    960

gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac   1020

aagggtggat ctggaggttc aggtggaagt ttggctacta cacttgaacg tattgagaag   1080

aactttgtca ttactgaccc aagattgcca gataatccca ttatattcgc gtccgatagt   1140

ttcttgcagt tgacagaata tagccgtgaa gaaattttgg gaagaaactg caggtttcta   1200

caaggtcctg aaactgatcg cgcgacagtg agaaaaatta gagatgccat agataaccaa   1260

acagaggtca ctgttcagct gattaattat acaaagagtg gtaaaaagtt ctggaacctc   1320

tttcacttgc agcctatgcg agatcagaag ggagatgtcc agtactttat tggggttcag   1380

ttggatggaa ctgagcatgt ccgagatgct gccgagagag agggagtcat gctgattaag   1440

aaaactgcag aaaatattga cgagctgctg aaggagctgg ccgacctgaa cctggactag   1500


<210>  75
<211>  309
<212>  DNA
<213>  Artificial

<220>
<223>  SV40 NLS AcrIIA4 , encoding

<400>  75
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    300

ctgaactag                                                            309


<210>  76
<211>  1023
<212>  DNA
<213>  Artificial

<220>
<223>  cMycP1A NLS-mCherry-AcrIIA4, encoding

<400>  76
atggccgcag caaagcgcgt gaagctggac gtgagcaagg gcgaggagga taacatggcc     60

atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag    120

ttcgagatcg agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg    180

aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg    240

tacggctcca aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc    300

ttccccgagg gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc    360

gtgacccagg actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc    420

accaacttcc cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc    480

tccgagcgga tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag    540

ctgaaggacg gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc    600

gtgcagctgc ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag    660

gactacacca tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg    720

gacgagctgt acaagggtgg atctggaggt tcaggtggaa gtaatattaa cgacctgatt    780

cgggaaataa agaacaagga ctacacggtc aagctgtcag gcaccgactc caactctata    840

acacagctca ttataagggt taataatgat ggcaacgaat atgtcatctc tgaatccgag    900

aatgaatcaa tcgtggagaa attcatcagt gcatttaaaa acggctggaa ccaggagtac    960

gaagatgagg aagagttcta caatgatatg caaactatca cactgaaatc tgagctgaac   1020

tag                                                                 1023


<210>  77
<211>  4848
<212>  DNA
<213>  Artificial

<220>
<223>  dCas9-VP64 transactivation luciferase reporter plasmid

<400>  77
gacgatatcc ctcgatcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg     60

agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc    120

agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag agaaaagtga    180

aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgag tttaccactc    240

cctatcagtg atagagaaaa gtgaaagtcg agtttaccac tccctatcag tgatagagaa    300

aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta    360

ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat    420

agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg    480

agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc actccctatc    540

agtgatagag aaaagtgaaa gtcgagctcg gtacgctatg gcatgcatgt gtcgcgacct    600

gcaggccctg aagttcatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt    660

gaccaccctg acctggggcg tgcagtgctt cgcccgctac cccgaccaca tgaagcagca    720

cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa    780

ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa    840

ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct    900

ggagtacaac gccatcagcg acaacgtcta tatcaccgcc gacaagcaga agaacggcat    960

caaggccaac ttcaagagct agccctatat aagcagagct cgtttagtga accgtcagat   1020

cgcctggaga cgccatccac gctgttttga cctccataga agacaccggg accgatccag   1080

cctccgcggc cccggtaccg aattcgccac catggaagat gccaaaaaca ttaagaaggg   1140

cccagcgcca ttctacccac tcgaagacgg gaccgccggc gagcagctgc acaaagccat   1200

gaagcgctac gccctggtgc ccggcaccat cgcctttacc gacgcacata tcgaggtgga   1260

cattacctac gccgagtact tcgagatgag cgttcggctg gcagaagcta tgaagcgcta   1320

tgggctgaat acaaaccatc ggatcgtggt gtgcagcgag aatagcttgc agttcttcat   1380

gcccgtgttg ggtgccctgt tcatcggtgt ggctgtggcc ccagctaacg acatctacaa   1440

cgagcgcgag ctgctgaaca gcatgggcat cagccagccc accgtcgtat tcgtgagcaa   1500

gaaagggctg caaaagatcc tcaacgtgca aaagaagcta ccgatcatac aaaagatcat   1560

catcatggat agcaagaccg actaccaggg cttccaaagc atgtacacct tcgtgacttc   1620

ccatttgcca cccggcttca acgagtacga cttcgtgccc gagagcttcg accgggacaa   1680

aaccatcgcc ctgatcatga acagtagtgg cagtaccgga ttgcccaagg gcgtagccct   1740

accgcaccgc accgcttgtg tccgattcag tcatgcccgc gaccccatct tcggcaacca   1800

gatcatcccc gacaccgcta tcctcagcgt ggtgccattt caccacggct tcggcatgtt   1860

caccacgctg ggctacttga tctgcggctt tcgggtcgtg ctcatgtacc gcttcgagga   1920

ggagctattc ttgcgcagct tgcaagacta taagattcaa tctgccctgc tggtgcccac   1980

actatttagc ttcttcgcta agagcactct catcgacaag tacgacctaa gcaacttgca   2040

cgagatcgcc agcggcgggg cgccgctcag caaggaggta ggtgaggccg tggccaaacg   2100

cttccaccta ccaggcatcc gccagggcta cggcctgaca gaaacaacca gcgccattct   2160

gatcaccccc gaaggggacg acaagcctgg cgcagtaggc aaggtggtgc ccttcttcga   2220

ggctaaggtg gtggacttgg acaccggtaa gacactgggt gtgaaccagc gcggcgagct   2280

gtgcgtccgt ggccccatga tcatgagcgg ctacgttaac aaccccgagg ctacaaacgc   2340

tctcatcgac aaggacggct ggctgcacag cggcgacatc gcctactggg acgaggacga   2400

gcacttcttc atcgtggacc ggctgaagag cctgatcaaa tacaagggct accaggtagc   2460

cccagccgaa ctggagagca tcctgctgca acaccccaac atcttcgacg ccggggtcgc   2520

cggcctgccc gacgacgatg ccggcgagct gcccgccgca gtcgtcgtgc tggaacacgg   2580

taaaaccatg accgagaagg agatcgtgga ctatgtggcc agccaggtta caaccgccaa   2640

gaagctgcgc ggtggtgttg tgttcgtgga cgaggtgcct aaaggactga ccggcaagtt   2700

ggacgcccgc aagatccgcg agattctcat taaggccaag aagtaaagat cttattaaag   2760

cagaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc   2820

acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta   2880

tcttatcatg tctggtcgac tctagaatct tccgcttcct cgctcactga ctcgctgcgc   2940

tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc   3000

acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg   3060

aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat   3120

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag   3180

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga   3240

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg   3300

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt   3360

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac   3420

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc   3480

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt   3540

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc   3600

ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc   3660

agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg   3720

aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag   3780

atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg   3840

tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt   3900

tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca   3960

tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca   4020

gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc   4080

tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt   4140

ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg   4200

gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc   4260

aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg   4320

ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga   4380

tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga   4440

ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta   4500

aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg   4560

ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact   4620

ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata   4680

agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt   4740

tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa   4800

ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtcgtc                4848


<210>  78
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between G62/W63

<400>  78

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Leu Ala Thr 
65                  70                  75                  80  


Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg Leu 
                85                  90                  95      


Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu Thr 
            100                 105                 110         


Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu Gln 
        115                 120                 125             


Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala Ile 
    130                 135                 140                 


Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys Ser 
145                 150                 155                 160 


Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp Gln 
                165                 170                 175     


Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr Glu 
            180                 185                 190         


His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys Lys 
        195                 200                 205             


Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Trp Asn Gln Glu 
    210                 215                 220                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  79
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between W63/N64

<400>  79

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Leu Ala 
65                  70                  75                  80  


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
                85                  90                  95      


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
            100                 105                 110         


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
        115                 120                 125             


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
    130                 135                 140                 


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
145                 150                 155                 160 


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
                165                 170                 175     


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
            180                 185                 190         


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
        195                 200                 205             


Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Asn Gln Glu 
    210                 215                 220                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  80
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between N64/Q65

<400>  80

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Leu 
65                  70                  75                  80  


Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro 
                85                  90                  95      


Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln 
            100                 105                 110         


Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe 
        115                 120                 125             


Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp 
    130                 135                 140                 


Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr 
145                 150                 155                 160 


Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg 
                165                 170                 175     


Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly 
            180                 185                 190         


Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile 
        195                 200                 205             


Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Gln Glu 
    210                 215                 220                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  81
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between Q65/E66

<400>  81

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp 
                85                  90                  95      


Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu 
            100                 105                 110         


Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg 
        115                 120                 125             


Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg 
    130                 135                 140                 


Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr 
145                 150                 155                 160 


Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met 
                165                 170                 175     


Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp 
            180                 185                 190         


Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu 
        195                 200                 205             


Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Glu 
    210                 215                 220                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  82
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between E66/Y67

<400>  82

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr 
                85                  90                  95      


Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe 
            100                 105                 110         


Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys 
        115                 120                 125             


Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile 
    130                 135                 140                 


Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn 
145                 150                 155                 160 


Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro 
                165                 170                 175     


Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu 
            180                 185                 190         


Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met 
        195                 200                 205             


Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu 
    210                 215                 220                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  83
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between Y67/E68

<400>  83

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Tyr Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile 
                85                  90                  95      


Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser 
            100                 105                 110         


Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn 
        115                 120                 125             


Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys 
    130                 135                 140                 


Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile 
145                 150                 155                 160 


Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln 
                165                 170                 175     


Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln 
            180                 185                 190         


Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val 
        195                 200                 205             


Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu 
    210                 215                 220                 


Leu Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  84
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between E68/D69

<400>  84

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Tyr Glu Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val 
                85                  90                  95      


Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp 
            100                 105                 110         


Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg 
        115                 120                 125             


Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg 
    130                 135                 140                 


Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu 
145                 150                 155                 160 


Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu 
                165                 170                 175     


Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val 
            180                 185                 190         


Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly 
        195                 200                 205             


Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys 
    210                 215                 220                 


Glu Leu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  85
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between E85/L86

<400>  85

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr 
                85                  90                  95      


Leu Lys Ser Glu Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe 
            100                 105                 110         


Val Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser 
        115                 120                 125             


Asp Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly 
    130                 135                 140                 


Arg Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val 
145                 150                 155                 160 


Arg Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln 
                165                 170                 175     


Leu Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His 
            180                 185                 190         


Leu Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly 
        195                 200                 205             


Val Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu 
    210                 215                 220                 


Gly Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala 
225                 230                 235                 240 


Lys Glu Leu Leu Asn 
                245 


<210>  86
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion between L86/N87

<400>  86

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr 
                85                  90                  95      


Leu Lys Ser Glu Leu Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn 
            100                 105                 110         


Phe Val Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala 
        115                 120                 125             


Ser Asp Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu 
    130                 135                 140                 


Gly Arg Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr 
145                 150                 155                 160 


Val Arg Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val 
                165                 170                 175     


Gln Leu Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe 
            180                 185                 190         


His Leu Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile 
        195                 200                 205             


Gly Val Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg 
    210                 215                 220                 


Glu Gly Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala 
225                 230                 235                 240 


Ala Lys Glu Leu Asn 
                245 


<210>  87
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  AsLOV2 AcrIIA4 insertion after N87

<400>  87

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr 
                85                  90                  95      


Leu Lys Ser Glu Leu Asn Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys 
            100                 105                 110         


Asn Phe Val Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe 
        115                 120                 125             


Ala Ser Asp Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile 
    130                 135                 140                 


Leu Gly Arg Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala 
145                 150                 155                 160 


Thr Val Arg Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr 
                165                 170                 175     


Val Gln Leu Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu 
            180                 185                 190         


Phe His Leu Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe 
        195                 200                 205             


Ile Gly Val Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu 
    210                 215                 220                 


Arg Glu Gly Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu 
225                 230                 235                 240 


Ala Ala Lys Glu Leu 
                245 


<210>  88
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQE[LOV2]YED - (#1)

<400>  88

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr 
                85                  90                  95      


Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe 
            100                 105                 110         


Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys 
        115                 120                 125             


Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile 
    130                 135                 140                 


Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn 
145                 150                 155                 160 


Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro 
                165                 170                 175     


Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu 
            180                 185                 190         


Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met 
        195                 200                 205             


Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu 
    210                 215                 220                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  89
<211>  247
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQEG[LOV2]GYED - (#2)

<400>  89

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile 
                85                  90                  95      


Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser 
            100                 105                 110         


Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn 
        115                 120                 125             


Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys 
    130                 135                 140                 


Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile 
145                 150                 155                 160 


Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln 
                165                 170                 175     


Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln 
            180                 185                 190         


Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val 
        195                 200                 205             


Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu 
    210                 215                 220                 


Leu Gly Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile 
225                 230                 235                 240 


Thr Leu Lys Ser Glu Leu Asn 
                245         


<210>  90
<211>  249
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQESG[LOV2]GSYED - (#3)

<400>  90

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Ser Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val 
                85                  90                  95      


Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp 
            100                 105                 110         


Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg 
        115                 120                 125             


Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg 
    130                 135                 140                 


Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu 
145                 150                 155                 160 


Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu 
                165                 170                 175     


Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val 
            180                 185                 190         


Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly 
        195                 200                 205             


Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys 
    210                 215                 220                 


Glu Leu Gly Ser Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln 
225                 230                 235                 240 


Thr Ile Thr Leu Lys Ser Glu Leu Asn 
                245                 


<210>  91
<211>  244
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQ[LOV2]YED - (#4)

<400>  91

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp 
                85                  90                  95      


Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu 
            100                 105                 110         


Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg 
        115                 120                 125             


Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg 
    130                 135                 140                 


Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr 
145                 150                 155                 160 


Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met 
                165                 170                 175     


Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp 
            180                 185                 190         


Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu 
        195                 200                 205             


Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Tyr 
    210                 215                 220                 


Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys 
225                 230                 235                 240 


Ser Glu Leu Asn 
                


<210>  92
<211>  246
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQG[LOV2]GYED - (#5)

<400>  92

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr 
                85                  90                  95      


Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe 
            100                 105                 110         


Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys 
        115                 120                 125             


Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile 
    130                 135                 140                 


Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn 
145                 150                 155                 160 


Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro 
                165                 170                 175     


Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu 
            180                 185                 190         


Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met 
        195                 200                 205             


Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu 
    210                 215                 220                 


Gly Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr 
225                 230                 235                 240 


Leu Lys Ser Glu Leu Asn 
                245     


<210>  93
<211>  248
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQSG[LOV2]GSYED - (#6)

<400>  93

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Ser Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile 
                85                  90                  95      


Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser 
            100                 105                 110         


Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn 
        115                 120                 125             


Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys 
    130                 135                 140                 


Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile 
145                 150                 155                 160 


Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln 
                165                 170                 175     


Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln 
            180                 185                 190         


Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val 
        195                 200                 205             


Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu 
    210                 215                 220                 


Leu Gly Ser Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr 
225                 230                 235                 240 


Ile Thr Leu Lys Ser Glu Leu Asn 
                245             


<210>  94
<211>  243
<212>  PRT
<213>  Artificial

<220>
<223>  GWN[LOV2]YED  - (#7)

<400>  94

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Leu 
65                  70                  75                  80  


Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro 
                85                  90                  95      


Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln 
            100                 105                 110         


Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe 
        115                 120                 125             


Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp 
    130                 135                 140                 


Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr 
145                 150                 155                 160 


Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg 
                165                 170                 175     


Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly 
            180                 185                 190         


Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile 
        195                 200                 205             


Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Tyr Glu 
    210                 215                 220                 


Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser 
225                 230                 235                 240 


Glu Leu Asn 
            


<210>  95
<211>  245
<212>  PRT
<213>  Artificial

<220>
<223>  GWNG[LOV2]GYED - (#8)

<400>  95

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gly 
65                  70                  75                  80  


Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp 
                85                  90                  95      


Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu 
            100                 105                 110         


Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg 
        115                 120                 125             


Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg 
    130                 135                 140                 


Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr 
145                 150                 155                 160 


Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met 
                165                 170                 175     


Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp 
            180                 185                 190         


Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu 
        195                 200                 205             


Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Gly 
    210                 215                 220                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
225                 230                 235                 240 


Lys Ser Glu Leu Asn 
                245 


<210>  96
<211>  247
<212>  PRT
<213>  Artificial

<220>
<223>  GWNSG[LOV2]GSYED - (#9)

<400>  96

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Ser 
65                  70                  75                  80  


Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr 
                85                  90                  95      


Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe 
            100                 105                 110         


Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys 
        115                 120                 125             


Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile 
    130                 135                 140                 


Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn 
145                 150                 155                 160 


Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro 
                165                 170                 175     


Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu 
            180                 185                 190         


Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met 
        195                 200                 205             


Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu 
    210                 215                 220                 


Gly Ser Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile 
225                 230                 235                 240 


Thr Leu Lys Ser Glu Leu Asn 
                245         


<210>  97
<211>  249
<212>  PRT
<213>  Artificial

<220>
<223>  GWNSGG[LOV2]GGSYED - (#10)

<400>  97

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Ser 
65                  70                  75                  80  


Gly Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile 
                85                  90                  95      


Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser 
            100                 105                 110         


Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn 
        115                 120                 125             


Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys 
    130                 135                 140                 


Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile 
145                 150                 155                 160 


Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln 
                165                 170                 175     


Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln 
            180                 185                 190         


Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val 
        195                 200                 205             


Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu 
    210                 215                 220                 


Leu Gly Gly Ser Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln 
225                 230                 235                 240 


Thr Ile Thr Leu Lys Ser Glu Leu Asn 
                245                 


<210>  98
<211>  249
<212>  PRT
<213>  Artificial

<220>
<223>  GWNGSG[LOV2]GSGYED - (#11)

<400>  98

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gly 
65                  70                  75                  80  


Ser Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile 
                85                  90                  95      


Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser 
            100                 105                 110         


Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn 
        115                 120                 125             


Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys 
    130                 135                 140                 


Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile 
145                 150                 155                 160 


Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln 
                165                 170                 175     


Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln 
            180                 185                 190         


Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val 
        195                 200                 205             


Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu 
    210                 215                 220                 


Leu Gly Ser Gly Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln 
225                 230                 235                 240 


Thr Ile Thr Leu Lys Ser Glu Leu Asn 
                245                 


<210>  99
<211>  257
<212>  PRT
<213>  Artificial

<220>
<223>  GWNGGSGGSG[LOV2]GSGGSGGYED - (#12)

<400>  99

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gly 
65                  70                  75                  80  


Gly Ser Gly Gly Ser Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu Lys 
                85                  90                  95      


Asn Phe Val Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile Phe 
            100                 105                 110         


Ala Ser Asp Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu Ile 
        115                 120                 125             


Leu Gly Arg Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg Ala 
    130                 135                 140                 


Thr Val Arg Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val Thr 
145                 150                 155                 160 


Val Gln Leu Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn Leu 
                165                 170                 175     


Phe His Leu Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr Phe 
            180                 185                 190         


Ile Gly Val Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala Glu 
        195                 200                 205             


Arg Glu Gly Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp Glu 
    210                 215                 220                 


Ala Ala Lys Glu Leu Gly Ser Gly Gly Ser Gly Gly Tyr Glu Asp Glu 
225                 230                 235                 240 


Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser Glu Leu 
                245                 250                 255     


Asn 
    


<210>  100
<211>  258
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQGGSGGSG[LOV2]GSGGSGGYED - (#13)

<400>  100

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Gly Gly Ser Gly Gly Ser Gly Leu Ala Thr Thr Leu Glu Arg Ile Glu 
                85                  90                  95      


Lys Asn Phe Val Ile Thr Asp Pro Arg Leu Pro Asp Asn Pro Ile Ile 
            100                 105                 110         


Phe Ala Ser Asp Ser Phe Leu Gln Leu Thr Glu Tyr Ser Arg Glu Glu 
        115                 120                 125             


Ile Leu Gly Arg Asn Cys Arg Phe Leu Gln Gly Pro Glu Thr Asp Arg 
    130                 135                 140                 


Ala Thr Val Arg Lys Ile Arg Asp Ala Ile Asp Asn Gln Thr Glu Val 
145                 150                 155                 160 


Thr Val Gln Leu Ile Asn Tyr Thr Lys Ser Gly Lys Lys Phe Trp Asn 
                165                 170                 175     


Leu Phe His Leu Gln Pro Met Arg Asp Gln Lys Gly Asp Val Gln Tyr 
            180                 185                 190         


Phe Ile Gly Val Gln Leu Asp Gly Thr Glu His Val Arg Asp Ala Ala 
        195                 200                 205             


Glu Arg Glu Gly Val Met Leu Ile Lys Lys Thr Ala Glu Asn Ile Asp 
    210                 215                 220                 


Glu Ala Ala Lys Glu Leu Gly Ser Gly Gly Ser Gly Gly Tyr Glu Asp 
225                 230                 235                 240 


Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser Glu 
                245                 250                 255     


Leu Asn 
        


<210>  101
<211>  300
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQE[UniRap]YED - (#Rap1)

<400>  101

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys 
                85                  90                  95      


Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly 
            100                 105                 110         


Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser 
        115                 120                 125             


Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly 
    130                 135                 140                 


Ala Thr Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys Asp Leu Leu 
145                 150                 155                 160 


Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Pro 
                165                 170                 175     


Pro Gly Pro Gly Ser Gly Leu Trp His Glu Met Trp His Glu Gly Leu 
            180                 185                 190         


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        195                 200                 205             


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    210                 215                 220                 


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
225                 230                 235                 240 


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Ser Ser Gly 
                245                 250                 255     


Gly Ser Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp 
            260                 265                 270         


Val Glu Leu Leu Lys Leu Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn 
        275                 280                 285             


Asp Met Gln Thr Ile Thr Leu Lys Ser Glu Leu Asn 
    290                 295                 300 


<210>  102
<211>  299
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQ[UniRap]YED - (#Rap2)

<400>  102

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Phe 
                85                  90                  95      


Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys 
            100                 105                 110         


Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val 
        115                 120                 125             


Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala 
    130                 135                 140                 


Thr Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys Asp Leu Leu Gln 
145                 150                 155                 160 


Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Pro Pro 
                165                 170                 175     


Gly Pro Gly Ser Gly Leu Trp His Glu Met Trp His Glu Gly Leu Glu 
            180                 185                 190         


Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe 
        195                 200                 205             


Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr 
    210                 215                 220                 


Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu 
225                 230                 235                 240 


Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Ser Ser Gly Gly 
                245                 250                 255     


Ser Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val 
            260                 265                 270         


Glu Leu Leu Lys Leu Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp 
        275                 280                 285             


Met Gln Thr Ile Thr Leu Lys Ser Glu Leu Asn 
    290                 295                 


<210>  103
<211>  298
<212>  PRT
<213>  Artificial

<220>
<223>  GWN[UniRap]YED - (#Rap3)

<400>  103

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Thr 
65                  70                  75                  80  


Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys Phe Asp 
                85                  90                  95      


Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly Lys Gln 
            100                 105                 110         


Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser Val Gly 
        115                 120                 125             


Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly Ala Thr 
    130                 135                 140                 


Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys Asp Leu Leu Gln Ala 
145                 150                 155                 160 


Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Pro Pro Gly 
                165                 170                 175     


Pro Gly Ser Gly Leu Trp His Glu Met Trp His Glu Gly Leu Glu Glu 
            180                 185                 190         


Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met Phe Glu 
        195                 200                 205             


Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln Thr Leu 
    210                 215                 220                 


Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met Glu Ala 
225                 230                 235                 240 


Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Ser Ser Gly Gly Ser 
                245                 250                 255     


Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp Val Glu 
            260                 265                 270         


Leu Leu Lys Leu Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met 
        275                 280                 285             


Gln Thr Ile Thr Leu Lys Ser Glu Leu Asn 
    290                 295             


<210>  104
<211>  302
<212>  PRT
<213>  Artificial

<220>
<223>  GWNSG[UniRap]GSYED - (#Rap4)

<400>  104

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Ser 
65                  70                  75                  80  


Gly Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys Lys 
                85                  90                  95      


Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu Gly 
            100                 105                 110         


Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met Ser 
        115                 120                 125             


Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr Gly 
    130                 135                 140                 


Ala Thr Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys Asp Leu Leu 
145                 150                 155                 160 


Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly Pro 
                165                 170                 175     


Pro Gly Pro Gly Ser Gly Leu Trp His Glu Met Trp His Glu Gly Leu 
            180                 185                 190         


Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly Met 
        195                 200                 205             


Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro Gln 
    210                 215                 220                 


Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu Met 
225                 230                 235                 240 


Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Ser Ser Gly 
                245                 250                 255     


Gly Ser Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe Asp 
            260                 265                 270         


Val Glu Leu Leu Lys Leu Glu Gly Ser Tyr Glu Asp Glu Glu Glu Phe 
        275                 280                 285             


Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser Glu Leu Asn 
    290                 295                 300         


<210>  105
<211>  303
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQSG[UniRap]GSYED - (#Rap5)

<400>  105

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Ser Gly Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys 
                85                  90                  95      


Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu 
            100                 105                 110         


Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met 
        115                 120                 125             


Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr 
    130                 135                 140                 


Gly Ala Thr Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys Asp Leu 
145                 150                 155                 160 


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly 
                165                 170                 175     


Pro Pro Gly Pro Gly Ser Gly Leu Trp His Glu Met Trp His Glu Gly 
            180                 185                 190         


Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly 
        195                 200                 205             


Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro 
    210                 215                 220                 


Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu 
225                 230                 235                 240 


Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Ser Ser 
                245                 250                 255     


Gly Gly Ser Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe 
            260                 265                 270         


Asp Val Glu Leu Leu Lys Leu Glu Gly Ser Tyr Glu Asp Glu Glu Glu 
        275                 280                 285             


Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser Glu Leu Asn 
    290                 295                 300             


<210>  106
<211>  306
<212>  PRT
<213>  Artificial

<220>
<223>  GWNQEGSG[UniRap]GSGYED - (#Rap6)

<400>  106

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Gly Ser Gly Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp 
                85                  90                  95      


Gly Lys Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe 
            100                 105                 110         


Met Leu Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala 
        115                 120                 125             


Gln Met Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr 
    130                 135                 140                 


Ala Tyr Gly Ala Thr Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys 
145                 150                 155                 160 


Asp Leu Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile 
                165                 170                 175     


Ser Gly Pro Pro Gly Pro Gly Ser Gly Leu Trp His Glu Met Trp His 
            180                 185                 190         


Glu Gly Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val 
        195                 200                 205             


Lys Gly Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg 
    210                 215                 220                 


Gly Pro Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg 
225                 230                 235                 240 


Asp Leu Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly 
                245                 250                 255     


Ser Ser Gly Gly Ser Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu 
            260                 265                 270         


Val Phe Asp Val Glu Leu Leu Lys Leu Glu Gly Ser Gly Tyr Glu Asp 
        275                 280                 285             


Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser Glu 
    290                 295                 300                 


Leu Asn 
305     


<210>  107
<211>  304
<212>  PRT
<213>  Artificial

<220>
<223>  GWNGSG[UniRap]GSGYED - (#Rap7)

<400>  107

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gly 
65                  70                  75                  80  


Ser Gly Thr Cys Val Val His Tyr Thr Gly Met Leu Glu Asp Gly Lys 
                85                  90                  95      


Lys Phe Asp Ser Ser Arg Asp Arg Asn Lys Pro Phe Lys Phe Met Leu 
            100                 105                 110         


Gly Lys Gln Glu Val Ile Arg Gly Trp Glu Glu Gly Val Ala Gln Met 
        115                 120                 125             


Ser Val Gly Gln Arg Ala Lys Leu Thr Ile Ser Pro Asp Tyr Ala Tyr 
    130                 135                 140                 


Gly Ala Thr Gly His Gly Ser Gly Ser Gly Ser Gly Val Lys Asp Leu 
145                 150                 155                 160 


Leu Gln Ala Trp Asp Leu Tyr Tyr His Val Phe Arg Arg Ile Ser Gly 
                165                 170                 175     


Pro Pro Gly Pro Gly Ser Gly Leu Trp His Glu Met Trp His Glu Gly 
            180                 185                 190         


Leu Glu Glu Ala Ser Arg Leu Tyr Phe Gly Glu Arg Asn Val Lys Gly 
        195                 200                 205             


Met Phe Glu Val Leu Glu Pro Leu His Ala Met Met Glu Arg Gly Pro 
    210                 215                 220                 


Gln Thr Leu Lys Glu Thr Ser Phe Asn Gln Ala Tyr Gly Arg Asp Leu 
225                 230                 235                 240 


Met Glu Ala Gln Glu Trp Cys Arg Lys Tyr Met Lys Ser Gly Ser Ser 
                245                 250                 255     


Gly Gly Ser Gly Ser Gly Ile Ile Pro Pro His Ala Thr Leu Val Phe 
            260                 265                 270         


Asp Val Glu Leu Leu Lys Leu Glu Gly Ser Gly Tyr Glu Asp Glu Glu 
        275                 280                 285             


Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser Glu Leu Asn 
    290                 295                 300                 


<210>  108
<211>  483
<212>  PRT
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4Aalpha (#8)

<400>  108

Met Ala Ala Ala Lys Arg Val Lys Leu Asp Val Ser Lys Gly Glu Glu 
1               5                   10                  15      


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
            20                  25                  30          


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
        35                  40                  45              


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
    50                  55                  60                  


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
65                  70                  75                  80  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
                85                  90                  95      


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
            100                 105                 110         


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
        115                 120                 125             


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
    130                 135                 140                 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
145                 150                 155                 160 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
                165                 170                 175     


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
            180                 185                 190         


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
        195                 200                 205             


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
    210                 215                 220                 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
225                 230                 235                 240 


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Leu Ala 
                245                 250                 255     


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
            260                 265                 270         


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
        275                 280                 285             


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
    290                 295                 300                 


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
305                 310                 315                 320 


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
                325                 330                 335     


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
            340                 345                 350         


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
        355                 360                 365             


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
    370                 375                 380                 


Lys Thr Ala Glu Asn Ile Asp Glu Ala Ala Lys Glu Leu Asn Ile Asn 
385                 390                 395                 400 


Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys Leu Ser 
                405                 410                 415     


Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val Asn Asn 
            420                 425                 430         


Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser Ile Val 
        435                 440                 445             


Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu Tyr Glu 
    450                 455                 460                 


Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser 
465                 470                 475                 480 


Glu Leu Asn 
            


<210>  109
<211>  483
<212>  PRT
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4Aalpha (#41)

<400>  109

Met Ala Ala Ala Lys Arg Val Lys Leu Asp Val Ser Lys Gly Glu Glu 
1               5                   10                  15      


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
            20                  25                  30          


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
        35                  40                  45              


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
    50                  55                  60                  


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
65                  70                  75                  80  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
                85                  90                  95      


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
            100                 105                 110         


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
        115                 120                 125             


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
    130                 135                 140                 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
145                 150                 155                 160 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
                165                 170                 175     


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
            180                 185                 190         


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
        195                 200                 205             


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
    210                 215                 220                 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
225                 230                 235                 240 


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Leu Ala 
                245                 250                 255     


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
            260                 265                 270         


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
        275                 280                 285             


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
    290                 295                 300                 


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
305                 310                 315                 320 


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
                325                 330                 335     


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
            340                 345                 350         


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
        355                 360                 365             


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
    370                 375                 380                 


Lys Thr Ala Glu Asn Ile Asp Glu Leu Ala Lys Ala Leu Asn Ile Asn 
385                 390                 395                 400 


Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys Leu Ser 
                405                 410                 415     


Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val Asn Asn 
            420                 425                 430         


Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser Ile Val 
        435                 440                 445             


Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu Tyr Glu 
    450                 455                 460                 


Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser 
465                 470                 475                 480 


Glu Leu Asn 
            


<210>  110
<211>  483
<212>  PRT
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4Aalpha (#51)

<400>  110

Met Ala Ala Ala Lys Arg Val Lys Leu Asp Val Ser Lys Gly Glu Glu 
1               5                   10                  15      


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
            20                  25                  30          


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
        35                  40                  45              


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
    50                  55                  60                  


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
65                  70                  75                  80  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
                85                  90                  95      


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
            100                 105                 110         


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
        115                 120                 125             


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
    130                 135                 140                 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
145                 150                 155                 160 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
                165                 170                 175     


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
            180                 185                 190         


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
        195                 200                 205             


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
    210                 215                 220                 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
225                 230                 235                 240 


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Leu Ala 
                245                 250                 255     


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
            260                 265                 270         


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
        275                 280                 285             


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
    290                 295                 300                 


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
305                 310                 315                 320 


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
                325                 330                 335     


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
            340                 345                 350         


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
        355                 360                 365             


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
    370                 375                 380                 


Lys Thr Ala Glu Asn Ile Glu Glu Leu Leu Lys Arg Leu Asn Ile Asn 
385                 390                 395                 400 


Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys Leu Ser 
                405                 410                 415     


Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val Asn Asn 
            420                 425                 430         


Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser Ile Val 
        435                 440                 445             


Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu Tyr Glu 
    450                 455                 460                 


Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser 
465                 470                 475                 480 


Glu Leu Asn 
            


<210>  111
<211>  481
<212>  PRT
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4Aalpha (#53-41)

<400>  111

Met Ala Ala Ala Lys Arg Val Lys Leu Asp Val Ser Lys Gly Glu Glu 
1               5                   10                  15      


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
            20                  25                  30          


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
        35                  40                  45              


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
    50                  55                  60                  


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
65                  70                  75                  80  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
                85                  90                  95      


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
            100                 105                 110         


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
        115                 120                 125             


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
    130                 135                 140                 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
145                 150                 155                 160 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
                165                 170                 175     


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
            180                 185                 190         


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
        195                 200                 205             


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
    210                 215                 220                 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
225                 230                 235                 240 


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Leu Ala 
                245                 250                 255     


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
            260                 265                 270         


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
        275                 280                 285             


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
    290                 295                 300                 


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
305                 310                 315                 320 


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
                325                 330                 335     


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
            340                 345                 350         


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
        355                 360                 365             


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
    370                 375                 380                 


Lys Thr Ala Glu Asn Ile Asp Glu Leu Ala Lys Ala Leu Asn Asp Leu 
385                 390                 395                 400 


Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys Leu Ser Gly Thr 
                405                 410                 415     


Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val Asn Asn Asp Gly 
            420                 425                 430         


Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser Ile Val Glu Lys 
        435                 440                 445             


Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu Tyr Glu Asp Glu 
    450                 455                 460                 


Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys Ser Glu Leu 
465                 470                 475                 480 


Asn 
    


<210>  112
<211>  484
<212>  PRT
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4Aalpha (#69-41)

<400>  112

Met Ala Ala Ala Lys Arg Val Lys Leu Asp Val Ser Lys Gly Glu Glu 
1               5                   10                  15      


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
            20                  25                  30          


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
        35                  40                  45              


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
    50                  55                  60                  


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
65                  70                  75                  80  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
                85                  90                  95      


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
            100                 105                 110         


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
        115                 120                 125             


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
    130                 135                 140                 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
145                 150                 155                 160 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
                165                 170                 175     


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
            180                 185                 190         


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
        195                 200                 205             


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
    210                 215                 220                 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
225                 230                 235                 240 


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Leu Ala 
                245                 250                 255     


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
            260                 265                 270         


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
        275                 280                 285             


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
    290                 295                 300                 


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
305                 310                 315                 320 


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
                325                 330                 335     


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
            340                 345                 350         


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
        355                 360                 365             


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
    370                 375                 380                 


Lys Thr Ala Glu Asn Ile Asp Glu Leu Ala Lys Ala Leu Ala Asn Ile 
385                 390                 395                 400 


Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys Leu 
                405                 410                 415     


Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val Asn 
            420                 425                 430         


Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser Ile 
        435                 440                 445             


Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu Tyr 
    450                 455                 460                 


Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys 
465                 470                 475                 480 


Ser Glu Leu Asn 
                


<210>  113
<211>  485
<212>  PRT
<213>  Artificial

<220>
<223>  (mCherry-) LOV2Jalpha-AcrIIA4Aalpha (#71-51)

<400>  113

Met Ala Ala Ala Lys Arg Val Lys Leu Asp Val Ser Lys Gly Glu Glu 
1               5                   10                  15      


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
            20                  25                  30          


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
        35                  40                  45              


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
    50                  55                  60                  


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
65                  70                  75                  80  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
                85                  90                  95      


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
            100                 105                 110         


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
        115                 120                 125             


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
    130                 135                 140                 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
145                 150                 155                 160 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
                165                 170                 175     


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
            180                 185                 190         


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
        195                 200                 205             


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
    210                 215                 220                 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
225                 230                 235                 240 


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Leu Ala 
                245                 250                 255     


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
            260                 265                 270         


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
        275                 280                 285             


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
    290                 295                 300                 


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
305                 310                 315                 320 


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
                325                 330                 335     


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
            340                 345                 350         


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
        355                 360                 365             


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
    370                 375                 380                 


Lys Thr Ala Glu Asn Ile Glu Glu Leu Leu Lys Arg Leu Gly Pro Asn 
385                 390                 395                 400 


Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys 
                405                 410                 415     


Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val 
            420                 425                 430         


Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser 
        435                 440                 445             


Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu 
    450                 455                 460                 


Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu 
465                 470                 475                 480 


Lys Ser Glu Leu Asn 
                485 


<210>  114
<211>  499
<212>  PRT
<213>  Artificial

<220>
<223>  cMyc NLS-AcrIIA4-mCherry-LEXY (pl76)

<400>  114

Met Pro Ala Ala Lys Arg Val Lys Leu Asp Met Gly Gly Ser Asn Ile 
1               5                   10                  15      


Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys Leu 
            20                  25                  30          


Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val Asn 
        35                  40                  45              


Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser Ile 
    50                  55                  60                  


Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu Tyr 
65                  70                  75                  80  


Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys 
                85                  90                  95      


Ser Glu Leu Asn Gly Gly Ser Gly Gly Ser Val Ser Lys Gly Glu Glu 
            100                 105                 110         


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
        115                 120                 125             


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
    130                 135                 140                 


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
145                 150                 155                 160 


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
                165                 170                 175     


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
            180                 185                 190         


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
        195                 200                 205             


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
    210                 215                 220                 


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
225                 230                 235                 240 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
                245                 250                 255     


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
            260                 265                 270         


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
        275                 280                 285             


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
    290                 295                 300                 


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
305                 310                 315                 320 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
                325                 330                 335     


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Leu Ala 
            340                 345                 350         


Thr Thr Leu Glu Arg Ile Glu Lys Asn Phe Val Ile Thr Asp Pro Arg 
        355                 360                 365             


Leu Pro Asp Asn Pro Ile Ile Phe Ala Ser Asp Ser Phe Leu Gln Leu 
    370                 375                 380                 


Thr Glu Tyr Ser Arg Glu Glu Ile Leu Gly Arg Asn Cys Arg Phe Leu 
385                 390                 395                 400 


Gln Gly Pro Glu Thr Asp Arg Ala Thr Val Arg Lys Ile Arg Asp Ala 
                405                 410                 415     


Ile Asp Asn Gln Thr Glu Val Thr Val Gln Leu Ile Asn Tyr Thr Lys 
            420                 425                 430         


Ser Gly Lys Lys Phe Trp Asn Leu Phe His Leu Gln Pro Met Arg Asp 
        435                 440                 445             


Gln Lys Gly Asp Val Gln Tyr Phe Ile Gly Val Gln Leu Asp Gly Thr 
    450                 455                 460                 


Glu His Val Arg Asp Ala Ala Glu Arg Glu Gly Val Met Leu Ile Lys 
465                 470                 475                 480 


Lys Thr Ala Glu Asn Ile Asp Glu Leu Leu Lys Glu Leu Ala Asp Leu 
                485                 490                 495     


Asn Leu Asp 
            


<210>  115
<211>  102
<212>  PRT
<213>  Artificial

<220>
<223>  SV40 NLS AcrIIA4

<400>  115

Met Ala Pro Lys Lys Lys Arg Lys Val Gly Gly Ser Gly Gly Ser Met 
1               5                   10                  15      


Asn Ile Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val 
            20                  25                  30          


Lys Leu Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg 
        35                  40                  45              


Val Asn Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu 
    50                  55                  60                  


Ser Ile Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln 
65                  70                  75                  80  


Glu Tyr Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr 
                85                  90                  95      


Leu Lys Ser Glu Leu Asn 
            100         


<210>  116
<211>  340
<212>  PRT
<213>  Artificial

<220>
<223>  cMycP1A NLS-mCherry-AcrIIA4

<400>  116

Met Ala Ala Ala Lys Arg Val Lys Leu Asp Val Ser Lys Gly Glu Glu 
1               5                   10                  15      


Asp Asn Met Ala Ile Ile Lys Glu Phe Met Arg Phe Lys Val His Met 
            20                  25                  30          


Glu Gly Ser Val Asn Gly His Glu Phe Glu Ile Glu Gly Glu Gly Glu 
        35                  40                  45              


Gly Arg Pro Tyr Glu Gly Thr Gln Thr Ala Lys Leu Lys Val Thr Lys 
    50                  55                  60                  


Gly Gly Pro Leu Pro Phe Ala Trp Asp Ile Leu Ser Pro Gln Phe Met 
65                  70                  75                  80  


Tyr Gly Ser Lys Ala Tyr Val Lys His Pro Ala Asp Ile Pro Asp Tyr 
                85                  90                  95      


Leu Lys Leu Ser Phe Pro Glu Gly Phe Lys Trp Glu Arg Val Met Asn 
            100                 105                 110         


Phe Glu Asp Gly Gly Val Val Thr Val Thr Gln Asp Ser Ser Leu Gln 
        115                 120                 125             


Asp Gly Glu Phe Ile Tyr Lys Val Lys Leu Arg Gly Thr Asn Phe Pro 
    130                 135                 140                 


Ser Asp Gly Pro Val Met Gln Lys Lys Thr Met Gly Trp Glu Ala Ser 
145                 150                 155                 160 


Ser Glu Arg Met Tyr Pro Glu Asp Gly Ala Leu Lys Gly Glu Ile Lys 
                165                 170                 175     


Gln Arg Leu Lys Leu Lys Asp Gly Gly His Tyr Asp Ala Glu Val Lys 
            180                 185                 190         


Thr Thr Tyr Lys Ala Lys Lys Pro Val Gln Leu Pro Gly Ala Tyr Asn 
        195                 200                 205             


Val Asn Ile Lys Leu Asp Ile Thr Ser His Asn Glu Asp Tyr Thr Ile 
    210                 215                 220                 


Val Glu Gln Tyr Glu Arg Ala Glu Gly Arg His Ser Thr Gly Gly Met 
225                 230                 235                 240 


Asp Glu Leu Tyr Lys Gly Gly Ser Gly Gly Ser Gly Gly Ser Asn Ile 
                245                 250                 255     


Asn Asp Leu Ile Arg Glu Ile Lys Asn Lys Asp Tyr Thr Val Lys Leu 
            260                 265                 270         


Ser Gly Thr Asp Ser Asn Ser Ile Thr Gln Leu Ile Ile Arg Val Asn 
        275                 280                 285             


Asn Asp Gly Asn Glu Tyr Val Ile Ser Glu Ser Glu Asn Glu Ser Ile 
    290                 295                 300                 


Val Glu Lys Phe Ile Ser Ala Phe Lys Asn Gly Trp Asn Gln Glu Tyr 
305                 310                 315                 320 


Glu Asp Glu Glu Glu Phe Tyr Asn Asp Met Gln Thr Ile Thr Leu Lys 
                325                 330                 335     


Ser Glu Leu Asn 
            340 


<210>  117
<211>  14547
<212>  DNA
<213>  Artificial

<220>
<223>  dCas9-VP64-2A-GFP reporter plasmid

<400>  117
gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg     60

atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt    120

gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc    180

tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac    240

attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat    300

atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg    360

acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt    420

tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag    480

tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc    540

attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag    600

tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt    660

ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc    720

accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg    780

gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct    840

ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt    900

aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac    960

tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc   1020

gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc   1080

ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa   1140

ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg   1200

ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata   1260

aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc   1320

tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga   1380

caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc   1440

aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca   1500

aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg   1560

agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga   1620

gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata   1680

ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg   1740

acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg   1800

ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag   1860

ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt   1920

tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt   1980

aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt   2040

aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag   2100

aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata   2160

acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta   2220

agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta   2280

tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa   2340

gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt   2400

gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat   2460

tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa   2520

agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag   2580

agatccagtt tggttaatta atgcaaagat ggataaagtt ttaaacagag aggaatcttt   2640

gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt   2700

gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa   2760

ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc   2820

gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc   2880

tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc   2940

ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt   3000

acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg   3060

cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc   3120

gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca   3180

tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg   3240

cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc   3300

gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg   3360

gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta   3420

tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat   3480

ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc   3540

gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat   3600

gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga   3660

gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt   3720

gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc   3780

tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc   3840

ttccatttca ggtgtcgtga cgtacggcca ccatgagccc caagaagaag agaaaggtgg   3900

aggccagcga caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg   3960

ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg   4020

accggcacag catcaagaag aacctgatcg gagccctgct gttcgacagc ggcgaaacag   4080

ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga   4140

tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc   4200

acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct   4260

tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga   4320

gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg   4380

cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca   4440

gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa   4500

accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga   4560

gcagacggct ggaaaatctg atcgcccagc tgcccggcga gaagaagaat ggcctgttcg   4620

gcaacctgat tgccctgagc ctgggcctga cccccaactt caagagcaac ttcgacctgg   4680

ccgaggatgc caaactgcag ctgagcaagg acacctacga cgacgacctg gacaacctgc   4740

tggcccagat cggcgaccag tacgccgacc tgtttctggc cgccaagaac ctgtccgacg   4800

ccatcctgct gagcgacatc ctgagagtga acaccgagat caccaaggcc cccctgagcg   4860

cctctatgat caagagatac gacgagcacc accaggacct gaccctgctg aaagctctcg   4920

tgcggcagca gctgcctgag aagtacaaag agattttctt cgaccagagc aagaacggct   4980

acgccggcta cattgacggc ggagccagcc aggaagagtt ctacaagttc atcaagccca   5040

tcctggaaaa gatggacggc accgaggaac tgctcgtgaa gctgaacaga gaggacctgc   5100

tgcggaagca gcggaccttc gacaacggca gcatccccca ccagatccac ctgggagagc   5160

tgcacgccat tctgcggcgg caggaagatt tttacccatt cctgaaggac aaccgggaaa   5220

agatcgagaa gatcctgacc ttccgcatcc cctactacgt gggccctctg gccaggggaa   5280

acagcagatt cgcctggatg accagaaaga gcgaggaaac catcaccccc tggaacttcg   5340

aggaagtggt ggacaagggc gcttccgccc agagcttcat cgagcggatg accaacttcg   5400

ataagaacct gcccaacgag aaggtgctgc ccaagcacag cctgctgtac gagtacttca   5460

ccgtgtataa cgagctgacc aaagtgaaat acgtgaccga gggaatgaga aagcccgcct   5520

tcctgagcgg cgagcagaaa aaggccatcg tggacctgct gttcaagacc aaccggaaag   5580

tgaccgtgaa gcagctgaaa gaggactact tcaagaaaat cgagtgcttc gactccgtgg   5640

aaatctccgg cgtggaagat cggttcaacg cctccctggg cacataccac gatctgctga   5700

aaattatcaa ggacaaggac ttcctggaca atgaggaaaa cgaggacatt ctggaagata   5760

tcgtgctgac cctgacactg tttgaggaca gagagatgat cgaggaacgg ctgaaaacct   5820

atgcccacct gttcgacgac aaagtgatga agcagctgaa gcggcggaga tacaccggct   5880

ggggcaggct gagccggaag ctgatcaacg gcatccggga caagcagtcc ggcaagacaa   5940

tcctggattt cctgaagtcc gacggcttcg ccaacagaaa cttcatgcag ctgatccacg   6000

acgacagcct gacctttaaa gaggacatcc agaaagccca ggtgtccggc cagggcgata   6060

gcctgcacga gcacattgcc aatctggccg gcagccccgc cattaagaag ggcatcctgc   6120

agacagtgaa ggtggtggac gagctcgtga aagtgatggg ccggcacaag cccgagaaca   6180

tcgtgatcga aatggccaga gagaaccaga ccacccagaa gggacagaag aacagccgcg   6240

agagaatgaa gcggatcgaa gagggcatca aagagctggg cagccagatc ctgaaagaac   6300

accccgtgga aaacacccag ctgcagaacg agaagctgta cctgtactac ctgcagaatg   6360

ggcgggatat gtacgtggac caggaactgg acatcaaccg gctgtccgac tacgatgtgg   6420

acgctatcgt gcctcagagc tttctgaagg acgactccat cgacaacaag gtgctgacca   6480

gaagcgacaa gaaccggggc aagagcgaca acgtgccctc cgaagaggtc gtgaagaaga   6540

tgaagaacta ctggcggcag ctgctgaacg ccaagctgat tacccagaga aagttcgaca   6600

atctgaccaa ggccgagaga ggcggcctga gcgaactgga taaggccggc ttcatcaaga   6660

gacagctggt ggaaacccgg cagatcacaa agcacgtggc acagatcctg gactcccgga   6720

tgaacactaa gtacgacgag aatgacaagc tgatccggga agtgaaagtg atcaccctga   6780

agtccaagct ggtgtccgat ttccggaagg atttccagtt ttacaaagtg cgcgagatca   6840

acaactacca ccacgcccac gacgcctacc tgaacgccgt cgtgggaacc gccctgatca   6900

aaaagtaccc taagctggaa agcgagttcg tgtacggcga ctacaaggtg tacgacgtgc   6960

ggaagatgat cgccaagagc gagcaggaaa tcggcaaggc taccgccaag tacttcttct   7020

acagcaacat catgaacttt ttcaagaccg agattaccct ggccaacggc gagatccgga   7080

agcggcctct gatcgagaca aacggcgaaa ccggggagat cgtgtgggat aagggccggg   7140

attttgccac cgtgcggaaa gtgctgagca tgccccaagt gaatatcgtg aaaaagaccg   7200

aggtgcagac aggcggcttc agcaaagagt ctatcctgcc caagaggaac agcgataagc   7260

tgatcgccag aaagaaggac tgggacccta agaagtacgg cggcttcgac agccccaccg   7320

tggcctattc tgtgctggtg gtggccaaag tggaaaaggg caagtccaag aaactgaaga   7380

gtgtgaaaga gctgctgggg atcaccatca tggaaagaag cagcttcgag aagaatccca   7440

tcgactttct ggaagccaag ggctacaaag aagtgaaaaa ggacctgatc atcaagctgc   7500

ctaagtactc cctgttcgag ctggaaaacg gccggaagag aatgctggcc tctgccggcg   7560

aactgcagaa gggaaacgaa ctggccctgc cctccaaata tgtgaacttc ctgtacctgg   7620

ccagccacta tgagaagctg aagggctccc ccgaggataa tgagcagaaa cagctgtttg   7680

tggaacagca caagcactac ctggacgaga tcatcgagca gatcagcgag ttctccaaga   7740

gagtgatcct ggccgacgct aatctggaca aagtgctgtc cgcctacaac aagcaccggg   7800

ataagcccat cagagagcag gccgagaata tcatccacct gtttaccctg accaatctgg   7860

gagcccctgc cgccttcaag tactttgaca ccaccatcga ccggaagagg tacaccagca   7920

ccaaagaggt gctggacgcc accctgatcc accagagcat caccggcctg tacgagacac   7980

ggatcgacct gtctcagctg ggaggcgaca gcgctggagg aggtggaagc ggaggaggag   8040

gaagcggagg aggaggtagc ggacctaaga aaaagaggaa ggtggcggcc gctggatccg   8100

gacgggctga cgcattggac gattttgatc tggatatgct gggaagtgac gccctcgatg   8160

attttgacct tgacatgctt ggttcggatg cccttgatga ctttgacctc gacatgctcg   8220

gcagtgacgc ccttgatgat ttcgacctgg acatgctgat taacgctagc ggcagtggag   8280

agggcagagg aagtctgcta acatgcggtg acgtcgagga gaatcctggc ccagtgagca   8340

agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa   8400

acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga   8460

ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca   8520

ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact   8580

tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg   8640

acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca   8700

tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt   8760

acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg   8820

tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc   8880

agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca   8940

cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt   9000

tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa gaattcgata   9060

tcaagcttat cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc   9120

ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg   9180

ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc   9240

tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg   9300

acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg   9360

ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga   9420

caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct   9480

ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg   9540

tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc   9600

ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc   9660

cgcatcgata ccgtcgacct cgagacctag aaaaacatgg agcaatcaca agtagcaata   9720

cagcagctac caatgctgat tgtgcctggc tagaagcaca agaggaggag gaggtgggtt   9780

ttccagtcac acctcaggta cctttaagac caatgactta caaggcagct gtagatctta   9840

gccacttttt aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagacaag   9900

atatccttga tctgtggatc taccacacac aaggctactt ccctgattgg cagaactaca   9960

caccagggcc agggatcaga tatccactga cctttggatg gtgctacaag ctagtaccag  10020

ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa cacccgcttg ttacaccctg  10080

tgagcctgca tgggatggat gacccggaga gagaagtatt agagtggagg tttgacagcc  10140

gcctagcatt tcatcacatg gcccgagagc tgcatccgga ctgtactggg tctctctggt  10200

tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc  10260

aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta  10320

actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagg gcccgtttaa  10380

acccgctgat cagcctcgac tgtgccttct agttgccagc catctgttgt ttgcccctcc  10440

cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag  10500

gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag  10560

gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct  10620

atggcttctg aggcggaaag aaccagctgg ggctctaggg ggtatcccca cgcgccctgt  10680

agcggcgcat taagcgcggc gggggggggg ggggggtggg gcaggacagc aagggggagg  10740

attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg  10800

aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg  10860

cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg  10920

ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc  10980

taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa  11040

aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc  11100

ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac  11160

tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt  11220

ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg  11280

tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca  11340

tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat  11400

gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc  11460

gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat  11520

ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt  11580

ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc  11640

tgatcagcac gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac  11700

aaggtgagga actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc  11760

gacgtcgccg gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg  11820

gaggacgact tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag  11880

gaccaggtgg tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg  11940

tacgccgagt ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg  12000

accgagatcg gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac  12060

tgcgtgcact tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc  12120

gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc  12180

ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct  12240

tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca  12300

ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg  12360

tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt  12420

tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt  12480

gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg  12540

ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg  12600

cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg  12660

cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat  12720

aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc  12780

gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc  12840

tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga  12900

agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt  12960

ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg  13020

taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc  13080

gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg  13140

gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc  13200

ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg  13260

ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc  13320

gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct  13380

caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt  13440

taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa  13500

aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa  13560

tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc  13620

tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct  13680

gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca  13740

gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt  13800

aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt  13860

gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc  13920

ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc  13980

tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt  14040

atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact  14100

ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc  14160

ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt  14220

ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg  14280

atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct  14340

gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa  14400

tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt  14460

ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc  14520

acatttcccc gaaaagtgcc acctgac                                      14547


<210>  118
<211>  3226
<212>  DNA
<213>  Artificial

<220>
<223>  TetO-targeting gRNA for dCas9-VP64 reporter plasmid

<400>  118
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc     60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga    120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc    180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc    240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag    300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa    360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac    420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg    480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg    540

gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg    600

taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tgggtaccga    660

gggcctattt cccatgattc cttcatattt gcatatacga tacaaggctg ttagagagat    720

aattggaatt aatttgactg taaacacaaa gatattagta caaaatacgt gacgtagaaa    780

gtaataattt cttgggtagt ttgcagtttt aaaattatgt tttaaaatgg actatcatat    840

gcttatcgta acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg    900

aaacaccgtc tctatcactg atagggaggt tttagagcta gaaatagcaa gttaaaataa    960

ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt tccgcggtgg   1020

agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta atcatggtca   1080

tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga   1140

agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg   1200

cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc   1260

caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac   1320

tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata   1380

cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa   1440

aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct   1500

gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa   1560

agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg   1620

cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca   1680

cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa   1740

ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg   1800

gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg   1860

tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg   1920

acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc   1980

tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag   2040

attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac   2100

gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc   2160

ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag   2220

taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt   2280

ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag   2340

ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca   2400

gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact   2460

ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca   2520

gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg   2580

tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc   2640

atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg   2700

gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca   2760

tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt   2820

atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc   2880

agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc   2940

ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca   3000

tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa   3060

aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat   3120

tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa   3180

aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccac                  3226


<210>  119
<211>  7601
<212>  DNA
<213>  Artificial

<220>
<223>  Cas9 Luciferase Cleavage Reporter Plasmid

<400>  119
ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc     60

attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga    120

gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc    180

caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc    240

ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag    300

cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa    360

agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac    420

cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg    480

caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggccactcc    540

ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc gacgcccggg    600

ctttgcccgg gcggcctcag tgagcgagcg agcgcgcaga gagggagtgg ccaactccat    660

cactaggggt tccttgtagt taatgattaa cccgccatgc tacttatcta cgtagccatg    720

ctctagagca agatctgcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg    780

ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga    840

aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca    900

accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc    960

aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc   1020

agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag   1080

gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc   1140

ttttgcaaaa agcttgattc ttctgacaca acagtctcga acttaagctg cagaagttgg   1200

tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg agaccaatag   1260

aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc tattggtctt   1320

actgacatcc actttgcctt tctctccaca ggtgtccact cccagttcaa ttacagctct   1380

taaggctaga gtacttaata cgactcacta taggctagcc accatggctt ccaaggtgta   1440

cgaccccgag caacgcaaac gcatgatcac tgggcctcag tggtgggctc gctgcaagca   1500

aatgaacgtg ctggactcct tcatcaacta ctatgattcc gagaagcacg ccgagaacgc   1560

cgtgattttt ctgcatggta acgctgcctc cagctacctg tggaggcacg tcgtgcctca   1620

catcgagccc gtggctagat gcatcatccc tgatctgatc ggaatgggta agtccggcaa   1680

gagcgggaat ggctcatatc gcctcctgga tcactacaag tacctcaccg cttggttcga   1740

gctgctgaac cttccaaaga aaatcatctt tgtgggccac gactgggggg cttgtctggc   1800

ctttcactac tcctacgagc accaagacaa gatcaaggcc atcgtccatg ctgagagtgt   1860

cgtggacgtg atcgagtcct gggacgagtg gcctgacatc gaggaggata tcgccctgat   1920

caagagcgaa gagggcgaga aaatggtgct tgagaataac ttcttcgtcg agaccatgct   1980

cccaagcaag atcatgcgga aactggagcc tgaggagttc gctgcctacc tggagccatt   2040

caaggagaag ggcgaggtta gacggcctac cctctcctgg cctcgcgaga tccctctcgt   2100

taagggaggc aagcccgacg tcgtccagat tgtccgcaac tacaacgcct accttcgggc   2160

cagcgacgat ctgcctaaga tgttcatcga gtccgaccct gggttctttt ccaacgctat   2220

tgtcgaggga gctaagaagt tccctaacac cgagttcgtg aaggtgaagg gcctccactt   2280

cagccaggag gacgctccag atgaaatggg taagtacatc aagagcttcg tggagcgcgt   2340

gctgaagaac gagcagtaat tctaggcgat cgctcgagcc cgggaattcg tttaaaccta   2400

gagcggccgc tggccgcaat aaaatatctt tattttcatt acatctgtgt gttggttttt   2460

tgtgtgagga tctaaatgag tcttcggacc tcgcgggggc cgcttaagcg gtggttaggg   2520

tttgtctgac gcggggggag ggggaaggaa cgaaacactc tcattcggag gcggctcggg   2580

gtttggtctt ggtggccacg ggcacgcaga agagcgccgc gatcctctta agcacccccc   2640

cgccctccgt ggaggcgggg gtttggtcgg cgggtggtaa ctggcgggcc gctgactcgg   2700

gcgggtcgcg cgccccagag tgtgaccttt tcggtctgct cgcagacccc cgggcggcgc   2760

cgccgcggcg gcgacgggct cgctgggtcc taggctccat ggggaccgta tacgtggaca   2820

ggctctggag catccgcacg actgcggtga tattaccgga gaccttctgc gggacgagcc   2880

gggtcacgcg gctgacgcgg agcgtccgtt gggcgacaaa caccaggacg gggcacaggt   2940

acactatctt gtcacccgga ggcgcgaggg actgcaggag cttcagggag tggcgcagct   3000

gcttcatccc cgtggcccgt tgctcgcgtt tgctggcggt gtccccggaa gaaatatatt   3060

tgcatgtctt tagttctatg atgacacaaa ccccgcccag cgtcttgtca ttggcgaatt   3120

cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc cacttcgcat attaaggtga   3180

cgcgtgtggc ctcgaacacc gagcgaccct gcagcgaccc gcttaaaagc ttggcattcc   3240

ggtactgttg gtaaagccac catggccgat gctaagaaca ttaagaaggg ccctgctccc   3300

ttctaccctc tggaggatgg caccgctggc gagcagctgc acaaggccat gaagaggtat   3360

gccctggtgc ctggcaccat tgccttcacc gatgcccaca ttgaggtgga catcacctat   3420

gccgagtact tcgagatgtc tgtgcgcctg gccgaggcca tgaagaggta cggcctgaac   3480

accaaccacc gcatcgtggt gtgctctgag aactctctgc agttcttcat gccagtgctg   3540

ggcgccctgt tcatcggagt ggccgtggcc cctgctaacg acatttacaa cgagcgcgag   3600

ctgctgaaca gcatgggcat ttctcagcct accgtggtgt tcgtgtctaa gaagggcctg   3660

cagaagatcc tgaacgtgca gaagaagctg cctatcatcc agaagatcat catcatggac   3720

tctaagaccg actaccaggg cttccagagc atgtacacat tcgtgacatc tcatctgcct   3780

cctggcttca acgagtacga cttcgtgcca gagtctttcg acagggacaa aaccattgcc   3840

ctgatcatga acagctctgg gtctaccggc ctgcctaagg gcgtggccct gcctcatcgc   3900

accgcctgtg tgcgcttctc tcacgcccgc gaccctattt tcggcaacca gatcatcccc   3960

gacaccgcta ttctgagcgt ggtgccattc caccacggct tcggcatgtt caccaccctg   4020

ggctacctga tttgcggctt tcgggtggtg ctgatgtacc gcttcgagga ggagctgttc   4080

ctgcgcagcc tgcaagacta caaaattcag tctgccctgc tggtgccaac cctgttcagc   4140

ttcttcgcta agagcaccct gatcgacaag tacgacctgt ctaacctgca cgagattgcc   4200

tctggcggcg ccccactgtc taaggaggtg ggcgaagccg tggccaagcg ctttcatctg   4260

ccaggcatcc gccagggcta cggcctgacc gagacaacca gcgccattct gattacccca   4320

gagggcgacg acaagcctgg cgccgtgggc aaggtggtgc cattcttcga ggccaaggtg   4380

gtggacctgg acaccggcaa gaccctggga gtgaaccagc gcggcgagct gtgtgtgcgc   4440

ggccctatga ttatgtccgg ctacgtgaat aaccctgagg ccacaaacgc cctgatcgac   4500

aaggacggct ggctgcactc tggcgacatt gcctactggg acgaggacga gcacttcttc   4560

atcgtggacc gcctgaagtc tctgatcaag tacaagggct accaggtggc cccagccgag   4620

ctggagtcta tcctgctgca gcaccctaac attttcgacg ccggagtggc cggcctgccc   4680

gacgacgatg ccggcgagct gcctgccgcc gtcgtcgtgc tggaacacgg caagaccatg   4740

accgagaagg agatcgtgga ctatgtggcc agccaggtga caaccgccaa gaagctgcgc   4800

ggcggagtgg tgttcgtgga cgaggtgccc aagggcctga ccggcaagct ggacgcccgc   4860

aagatccgcg agatcctgat caaggctaag aaaggcggca agatcgccgt gtaataattc   4920

tagagtcggg gcggccggcc gcttcgagca gacatgataa gatacattga tgagtttgga   4980

caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt   5040

gctttatttg taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat   5100

tttatgtttc aggttcaggg ggaggtgtgg gaggtttttt aaagcaagta aaacctctac   5160

aaatgtggta aaatcgatac tagtgcaaaa aaagcaccga ctcggtgcca ctttttcaag   5220

ttgataacgg actagcctta tttaaacttg ctatgctgtt tccagcatag ctcttaaacg   5280

gtagtcggtc ttagagtccg gtgtaccgtg gtctcataca gaacttataa gattcccaaa   5340

tccaaagaca tttcacgttt atggtgattt cccagaacac atagcgacat gcaaatatgg   5400

gcatcgataa actagttgct ctagagcatg gctacgtaga taagtagcat ggcgggttaa   5460

tcattaacta caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct   5520

cgctcactga ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct   5580

cagtgagcga gcgagcgcgc agagagggag tggccagctg cattaatgaa tcggccaacg   5640

cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct   5700

gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt   5760

atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc   5820

caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga   5880

gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata   5940

ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac   6000

cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg   6060

taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc   6120

cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag   6180

acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt   6240

aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt   6300

atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg   6360

atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac   6420

gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca   6480

gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac   6540

ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac   6600

ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt   6660

tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt   6720

accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt   6780

atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc   6840

cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa   6900

tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg   6960

tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt   7020

gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc   7080

agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt   7140

aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg   7200

gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac   7260

tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc   7320

gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt   7380

tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg   7440

aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag   7500

catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa   7560

acaaataggg gttccgcgca catttccccg aaaagtgcca c                       7601


<210>  120
<211>  429
<212>  DNA
<213>  Artificial

<220>
<223>  LOV2 encoding alternative codon usage

<400>  120
ttggctacta cacttgaacg tattgagaag aactttgtca ttactgaccc aagattgcca     60

gataatccca ttatattcgc gtccgatagt ttcttgcagt tgacagaata tagccgtgaa    120

gaaattttgg gaagaaactg caggtttcta caaggtcctg aaactgatcg cgcgacagtg    180

agaaaaatta gagatgccat agataaccaa acagaggtca ctgttcagct gattaattat    240

acaaagagtg gtaaaaagtt ctggaacctc tttcacttgc agcctatgcg agatcagaag    300

ggagatgtcc agtactttat tggggttcag ttggatggaa ctgagcatgt ccgagatgct    360

gccgagagag agggagtcat gctgattaag aaaactgcag aaaatattga cgaagcggcg    420

aaggagttg                                                            429


<210>  121
<211>  5
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #4-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (2)..(3)
<223>  site of receptor domain insertion

<400>  121

Gly Trp Tyr Glu Asp 
1               5   


<210>  122
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #5-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (1)..(2)
<223>  site of receptor domain insertion

<400>  122

Gly Gly Ser Gly Gly Ser Gly Gly Tyr Glu Asp 
1               5                   10      


<210>  123
<211>  7
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #6-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (3)..(4)
<223>  site of receptor domain insertion

<400>  123

Gly Trp Gly Gly Tyr Glu Asp 
1               5           


<210>  124
<211>  9
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #7-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (4)..(5)
<223>  site of receptor domain insertion

<400>  124

Gly Trp Ser Gly Gly Ser Tyr Glu Asp 
1               5                   


<210>  125
<211>  11
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #8-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  site of receptor domain insertion

<400>  125

Gly Trp Gly Ser Gly Gly Ser Gly Tyr Glu Asp 
1               5                   10      


<210>  126
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #9-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  site of receptor domain insertion

<400>  126

Gly Trp Gly Gly Ser Gly Gly Ser Gly Gly Tyr Glu Asp 
1               5                   10              


<210>  127
<211>  15
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #10-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  site of receptor domain insertion

<400>  127

Gly Trp Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Tyr Glu Asp 
1               5                   10                  15  


<210>  128
<211>  17
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #11-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (8)..(9)
<223>  site of receptor domain insertion

<400>  128

Gly Trp Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Tyr Glu 
1               5                   10                  15      


Asp 
    


<210>  129
<211>  19
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #12-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (9)..(10)
<223>  site of receptor domain insertion

<400>  129

Gly Trp Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly 
1               5                   10                  15      


Tyr Glu Asp 
            


<210>  130
<211>  8
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #13-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (4)..(5)
<223>  site of receptor domain insertion

<400>  130

Gly Trp Asn Gly Gly Tyr Glu Asp 
1               5               


<210>  131
<211>  10
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #14-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (5)..(6)
<223>  site of receptor domain insertion

<400>  131

Gly Trp Asn Ser Gly Gly Ser Tyr Glu Asp 
1               5                   10  


<210>  132
<211>  12
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #15-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (6)..(7)
<223>  site of receptor domain insertion

<400>  132

Gly Trp Asn Gly Ser Gly Gly Ser Gly Tyr Glu Asp 
1               5                   10          


<210>  133
<211>  13
<212>  PRT
<213>  Artificial

<220>
<223>  fusion site corresponding to amino acids 62 to 69 of ACRIIA4, 
       variant #16-Fig.7


<220>
<221>  MISC_FEATURE
<222>  (7)..(8)
<223>  site of receptor domain insertion

<400>  133

Gly Trp Asn Gln Gly Ser Gly Gly Ser Gly Tyr Glu Asp 
1               5                   10              


<210>  134
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 1

<400>  134
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

gagcttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    300

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    360

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    420

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    480

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    540

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    600

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    660

gcgaaagagt tgtacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  135
<211>  735
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 2

<400>  135
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    300

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    360

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    420

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    480

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    540

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    600

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    660

aaagagttgt acgaagatga ggaagagttc tacaatgata tgcaaactat cacactgaaa    720

tctgagctga actag                                                     735


<210>  136
<211>  732
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 3

<400>  136
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacctt    240

gctacaactt tggagcggat tgagaagaat ttcgtcataa ctgatcccag gttgcctgat    300

aatcctataa tattcgcctc cgatagtttt cttcaactta cggagtactc cagagaggag    360

atactgggaa ggaattgcag attcctccaa ggtccagaaa ctgatagagc tactgtgagg    420

aagatacggg atgccattga caatcaaact gaagtgacag tgcaacttat caattacacc    480

aagtcaggga aaaaattctg gaacctgttc catttgcaac ccatgaggga ccaaaagggc    540

gatgtgcaat atttcatagg cgttcaactt gatggtactg aacacgtcag agatgcggcg    600

gagcgagaag gagtgatgct gattaagaaa acagcagaga atatcgacga agcggcgaaa    660

gagttgtacg aagatgagga agagttctac aatgatatgc aaactatcac actgaaatct    720

gagctgaact ag                                                        732


<210>  137
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 4

<400>  137
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  138
<211>  747
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 5

<400>  138
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ccttgctaca    240

actttggagc ggattgagaa gaatttcgtc ataactgatc ccaggttgcc tgataatcct    300

ataatattcg cctccgatag ttttcttcaa cttacggagt actccagaga ggagatactg    360

ggaaggaatt gcagattcct ccaaggtcca gaaactgata gagctactgt gaggaagata    420

cgggatgcca ttgacaatca aactgaagtg acagtgcaac ttatcaatta caccaagtca    480

gggaaaaaat tctggaacct gttccatttg caacccatga gggaccaaaa gggcgatgtg    540

caatatttca taggcgttca acttgatggt actgaacacg tcagagatgc ggcggagcga    600

gaaggagtga tgctgattaa gaaaacagca gagaatatcg acgaagcggc gaaagagttg    660

ggcagcggcg gcagcggcgg ctacgaagat gaggaagagt tctacaatga tatgcaaact    720

atcacactga aatctgagct gaactag                                        747


<210>  139
<211>  735
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 6

<400>  139
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggggcctt    240

gctacaactt tggagcggat tgagaagaat ttcgtcataa ctgatcccag gttgcctgat    300

aatcctataa tattcgcctc cgatagtttt cttcaactta cggagtactc cagagaggag    360

atactgggaa ggaattgcag attcctccaa ggtccagaaa ctgatagagc tactgtgagg    420

aagatacggg atgccattga caatcaaact gaagtgacag tgcaacttat caattacacc    480

aagtcaggga aaaaattctg gaacctgttc catttgcaac ccatgaggga ccaaaagggc    540

gatgtgcaat atttcatagg cgttcaactt gatggtactg aacacgtcag agatgcggcg    600

gagcgagaag gagtgatgct gattaagaaa acagcagaga atatcgacga agcggcgaaa    660

gagttgggct acgaagatga ggaagagttc tacaatgata tgcaaactat cacactgaaa    720

tctgagctga actag                                                     735


<210>  140
<211>  741
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 7

<400>  140
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggagcggc    240

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    300

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    360

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    420

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    480

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    540

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    600

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    660

aaagagttgg gcagctacga agatgaggaa gagttctaca atgatatgca aactatcaca    720

ctgaaatctg agctgaacta g                                              741


<210>  141
<211>  747
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 8

<400>  141
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggggcagc    240

ggccttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    300

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    360

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    420

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    480

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    540

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    600

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    660

gcgaaagagt tgggcagcgg ctacgaagat gaggaagagt tctacaatga tatgcaaact    720

atcacactga aatctgagct gaactag                                        747


<210>  142
<211>  753
<212>  DNA
<213>  Artificial

<220>
<223>  Atggccccgaagaaaaagcgaaaggtgggcggaagcggaggttccatgaacattaacgacctgat
       tcgggaaataaagaacaaggactacacggtcaagctgtcaggcaccgactccaactctataacac
       agctcattataagggttaataatgatggcaacgaatatgtcatctctgaatccgagaatgaatca
       atcgtggagaaattcatcagtgcatttaaaaacggctggggcggcagcggccttgctacaacttt

<400>  142
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggggcggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggcggctac gaagatgagg aagagttcta caatgatatg    720

caaactatca cactgaaatc tgagctgaac tag                                 753


<210>  143
<211>  759
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 10

<400>  143
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggagcggc    240

ggcagcggcc ttgctacaac tttggagcgg attgagaaga atttcgtcat aactgatccc    300

aggttgcctg ataatcctat aatattcgcc tccgatagtt ttcttcaact tacggagtac    360

tccagagagg agatactggg aaggaattgc agattcctcc aaggtccaga aactgataga    420

gctactgtga ggaagatacg ggatgccatt gacaatcaaa ctgaagtgac agtgcaactt    480

atcaattaca ccaagtcagg gaaaaaattc tggaacctgt tccatttgca acccatgagg    540

gaccaaaagg gcgatgtgca atatttcata ggcgttcaac ttgatggtac tgaacacgtc    600

agagatgcgg cggagcgaga aggagtgatg ctgattaaga aaacagcaga gaatatcgac    660

gaagcggcga aagagttggg cagcggcggc agctacgaag atgaggaaga gttctacaat    720

gatatgcaaa ctatcacact gaaatctgag ctgaactag                           759


<210>  144
<211>  765
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 11

<400>  144
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggggcagc    240

ggcggcagcg gccttgctac aactttggag cggattgaga agaatttcgt cataactgat    300

cccaggttgc ctgataatcc tataatattc gcctccgata gttttcttca acttacggag    360

tactccagag aggagatact gggaaggaat tgcagattcc tccaaggtcc agaaactgat    420

agagctactg tgaggaagat acgggatgcc attgacaatc aaactgaagt gacagtgcaa    480

cttatcaatt acaccaagtc agggaaaaaa ttctggaacc tgttccattt gcaacccatg    540

agggaccaaa agggcgatgt gcaatatttc ataggcgttc aacttgatgg tactgaacac    600

gtcagagatg cggcggagcg agaaggagtg atgctgatta agaaaacagc agagaatatc    660

gacgaagcgg cgaaagagtt gggcagcggc ggcagcggct acgaagatga ggaagagttc    720

tacaatgata tgcaaactat cacactgaaa tctgagctga actag                    765


<210>  145
<211>  771
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 12

<400>  145
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggggcggc    240

agcggcggca gcggccttgc tacaactttg gagcggattg agaagaattt cgtcataact    300

gatcccaggt tgcctgataa tcctataata ttcgcctccg atagttttct tcaacttacg    360

gagtactcca gagaggagat actgggaagg aattgcagat tcctccaagg tccagaaact    420

gatagagcta ctgtgaggaa gatacgggat gccattgaca atcaaactga agtgacagtg    480

caacttatca attacaccaa gtcagggaaa aaattctgga acctgttcca tttgcaaccc    540

atgagggacc aaaagggcga tgtgcaatat ttcataggcg ttcaacttga tggtactgaa    600

cacgtcagag atgcggcgga gcgagaagga gtgatgctga ttaagaaaac agcagagaat    660

atcgacgaag cggcgaaaga gttgggcagc ggcggcagcg gcggctacga agatgaggaa    720

gagttctaca atgatatgca aactatcaca ctgaaatctg agctgaacta g             771


<210>  146
<211>  738
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 13

<400>  146
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    300

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    360

gagatactgg gaaggaattg cagattcctc caaggtccag aaactgatag agctactgtg    420

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    480

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    540

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    600

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    660

aaagagttgg gctacgaaga tgaggaagag ttctacaatg atatgcaaac tatcacactg    720

aaatctgagc tgaactag                                                  738


<210>  147
<211>  744
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 14

<400>  147
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacagc    240

ggccttgcta caactttgga gcggattgag aagaatttcg tcataactga tcccaggttg    300

cctgataatc ctataatatt cgcctccgat agttttcttc aacttacgga gtactccaga    360

gaggagatac tgggaaggaa ttgcagattc ctccaaggtc cagaaactga tagagctact    420

gtgaggaaga tacgggatgc cattgacaat caaactgaag tgacagtgca acttatcaat    480

tacaccaagt cagggaaaaa attctggaac ctgttccatt tgcaacccat gagggaccaa    540

aagggcgatg tgcaatattt cataggcgtt caacttgatg gtactgaaca cgtcagagat    600

gcggcggagc gagaaggagt gatgctgatt aagaaaacag cagagaatat cgacgaagcg    660

gcgaaagagt tgggcagcta cgaagatgag gaagagttct acaatgatat gcaaactatc    720

acactgaaat ctgagctgaa ctag                                           744


<210>  148
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 15

<400>  148
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  149
<211>  753
<212>  DNA
<213>  Artificial

<220>
<223>  AcrIIA4-LOV2 hybrid 16

<400>  149
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

ggcagcggcc ttgctacaac tttggagcgg attgagaaga atttcgtcat aactgatccc    300

aggttgcctg ataatcctat aatattcgcc tccgatagtt ttcttcaact tacggagtac    360

tccagagagg agatactggg aaggaattgc agattcctcc aaggtccaga aactgataga    420

gctactgtga ggaagatacg ggatgccatt gacaatcaaa ctgaagtgac agtgcaactt    480

atcaattaca ccaagtcagg gaaaaaattc tggaacctgt tccatttgca acccatgagg    540

gaccaaaagg gcgatgtgca atatttcata ggcgttcaac ttgatggtac tgaacacgtc    600

agagatgcgg cggagcgaga aggagtgatg ctgattaaga aaacagcaga gaatatcgac    660

gaagcggcga aagagttggg cagcggctac gaagatgagg aagagttcta caatgatatg    720

caaactatca cactgaaatc tgagctgaac tag                                 753


<210>  150
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (LOV:C450A)

<400>  150
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga atgctagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  151
<211>  732
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 3 (LOV:C450A)

<400>  151
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacctt    240

gctacaactt tggagcggat tgagaagaat ttcgtcataa ctgatcccag gttgcctgat    300

aatcctataa tattcgcctc cgatagtttt cttcaactta cggagtactc cagagaggag    360

atactgggaa ggaatgctag attcctccaa ggtccagaaa ctgatagagc tactgtgagg    420

aagatacggg atgccattga caatcaaact gaagtgacag tgcaacttat caattacacc    480

aagtcaggga aaaaattctg gaacctgttc catttgcaac ccatgaggga ccaaaagggc    540

gatgtgcaat atttcatagg cgttcaactt gatggtactg aacacgtcag agatgcggcg    600

gagcgagaag gagtgatgct gattaagaaa acagcagaga atatcgacga agcggcgaaa    660

gagttgtacg aagatgagga agagttctac aatgatatgc aaactatcac actgaaatct    720

gagctgaact ag                                                        732


<210>  152
<211>  735
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 2 (LOV:C450A)

<400>  152
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaaccag    240

cttgctacaa ctttggagcg gattgagaag aatttcgtca taactgatcc caggttgcct    300

gataatccta taatattcgc ctccgatagt tttcttcaac ttacggagta ctccagagag    360

gagatactgg gaaggaatgc tagattcctc caaggtccag aaactgatag agctactgtg    420

aggaagatac gggatgccat tgacaatcaa actgaagtga cagtgcaact tatcaattac    480

accaagtcag ggaaaaaatt ctggaacctg ttccatttgc aacccatgag ggaccaaaag    540

ggcgatgtgc aatatttcat aggcgttcaa cttgatggta ctgaacacgt cagagatgcg    600

gcggagcgag aaggagtgat gctgattaag aaaacagcag agaatatcga cgaagcggcg    660

aaagagttgt acgaagatga ggaagagttc tacaatgata tgcaaactat cacactgaaa    720

tctgagctga actag                                                     735


<210>  153
<211>  771
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 12 (LOV:C450A)

<400>  153
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggggcggc    240

agcggcggca gcggccttgc tacaactttg gagcggattg agaagaattt cgtcataact    300

gatcccaggt tgcctgataa tcctataata ttcgcctccg atagttttct tcaacttacg    360

gagtactcca gagaggagat actgggaagg aatgctagat tcctccaagg tccagaaact    420

gatagagcta ctgtgaggaa gatacgggat gccattgaca atcaaactga agtgacagtg    480

caacttatca attacaccaa gtcagggaaa aaattctgga acctgttcca tttgcaaccc    540

atgagggacc aaaagggcga tgtgcaatat ttcataggcg ttcaacttga tggtactgaa    600

cacgtcagag atgcggcgga gcgagaagga gtgatgctga ttaagaaaac agcagagaat    660

atcgacgaag cggcgaaaga gttgggcagc ggcggcagcg gcggctacga agatgaggaa    720

gagttctaca atgatatgca aactatcaca ctgaaatctg agctgaacta g             771


<210>  154
<211>  753
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 9 (LOV:C450A)

<400>  154
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggggcggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaatgctaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggcggctac gaagatgagg aagagttcta caatgatatg    720

caaactatca cactgaaatc tgagctgaac tag                                 753


<210>  155
<211>  759
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 10 (LOV:C450A)

<400>  155
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggagcggc    240

ggcagcggcc ttgctacaac tttggagcgg attgagaaga atttcgtcat aactgatccc    300

aggttgcctg ataatcctat aatattcgcc tccgatagtt ttcttcaact tacggagtac    360

tccagagagg agatactggg aaggaatgct agattcctcc aaggtccaga aactgataga    420

gctactgtga ggaagatacg ggatgccatt gacaatcaaa ctgaagtgac agtgcaactt    480

atcaattaca ccaagtcagg gaaaaaattc tggaacctgt tccatttgca acccatgagg    540

gaccaaaagg gcgatgtgca atatttcata ggcgttcaac ttgatggtac tgaacacgtc    600

agagatgcgg cggagcgaga aggagtgatg ctgattaaga aaacagcaga gaatatcgac    660

gaagcggcga aagagttggg cagcggcggc agctacgaag atgaggaaga gttctacaat    720

gatatgcaaa ctatcacact gaaatctgag ctgaactag                           759


<210>  156
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (LOV:G528A, N538E)

<400>  156
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaagcag tgatgctgat taagaaaaca gcagaggaga tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  157
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (LOV:I532A)

<400>  157
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctggc aaagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  158
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (LOV:T406/407A,G528A,N538E)

<400>  158
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

gcagcgttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaagcag tgatgctgat taagaaaaca gcagaggaga tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  159
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (LOV:T406/407A,I532A)

<400>  159
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

gcagcgttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctggc aaagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  160
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (LOV:T406/407A)

<400>  160
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

gcagcgttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  161
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 15 (LOV:G528A, N538E)

<400>  161
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagc agtgatgctg attaagaaaa cagcagagga gatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  162
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 15 (LOV:I532A)

<400>  162
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg gcaaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  163
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 15 (LOV:T406/407A,G528A,N538E)

<400>  163
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggccttg ctgcagcgtt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagc agtgatgctg attaagaaaa cagcagagga gatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  164
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 15 (LOV:T406/407A,I532A)

<400>  164
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggccttg ctgcagcgtt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg gcaaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  165
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 15 (LOV:T406/407A)

<400>  165
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaacggc    240

agcggccttg ctgcagcgtt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  166
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 15 (Acr:N64K)

<400>  166
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggaagggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  167
<211>  750
<212>  DNA
<213>  Artificial

<220>
<223>  Acr-LOV hybrid 15 (Acr:N64R)

<400>  167
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggagaggc    240

agcggccttg ctacaacttt ggagcggatt gagaagaatt tcgtcataac tgatcccagg    300

ttgcctgata atcctataat attcgcctcc gatagttttc ttcaacttac ggagtactcc    360

agagaggaga tactgggaag gaattgcaga ttcctccaag gtccagaaac tgatagagct    420

actgtgagga agatacggga tgccattgac aatcaaactg aagtgacagt gcaacttatc    480

aattacacca agtcagggaa aaaattctgg aacctgttcc atttgcaacc catgagggac    540

caaaagggcg atgtgcaata tttcataggc gttcaacttg atggtactga acacgtcaga    600

gatgcggcgg agcgagaagg agtgatgctg attaagaaaa cagcagagaa tatcgacgaa    660

gcggcgaaag agttgggcag cggctacgaa gatgaggaag agttctacaa tgatatgcaa    720

actatcacac tgaaatctga gctgaactag                                     750


<210>  168
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:E45K)

<400>  168
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctaag    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  169
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:K18Q)

<400>  169
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtccagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  170
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:S46D)

<400>  170
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

gacgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  171
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:T16F)

<400>  171
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac ttcgtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  172
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:T16Y)

<400>  172
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac tatgtcaagc tgtcaggcac cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  173
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:T22H)

<400>  173
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcca cgactccaac    120

tctataacac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  174
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:T28E)

<400>  174
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctatagaac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  175
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:T28N)

<400>  175
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctataaacc agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


<210>  176
<211>  729
<212>  DNA
<213>  Artificial

<220>
<223>  CASANOVA (Acr:T28Q)

<400>  176
atggccccga agaaaaagcg aaaggtgggc ggaagcggag gttccatgaa cattaacgac     60

ctgattcggg aaataaagaa caaggactac acggtcaagc tgtcaggcac cgactccaac    120

tctatacaac agctcattat aagggttaat aatgatggca acgaatatgt catctctgaa    180

tccgagaatg aatcaatcgt ggagaaattc atcagtgcat ttaaaaacgg ctggcttgct    240

acaactttgg agcggattga gaagaatttc gtcataactg atcccaggtt gcctgataat    300

cctataatat tcgcctccga tagttttctt caacttacgg agtactccag agaggagata    360

ctgggaagga attgcagatt cctccaaggt ccagaaactg atagagctac tgtgaggaag    420

atacgggatg ccattgacaa tcaaactgaa gtgacagtgc aacttatcaa ttacaccaag    480

tcagggaaaa aattctggaa cctgttccat ttgcaaccca tgagggacca aaagggcgat    540

gtgcaatatt tcataggcgt tcaacttgat ggtactgaac acgtcagaga tgcggcggag    600

cgagaaggag tgatgctgat taagaaaaca gcagagaata tcgacgaagc ggcgaaagag    660

ttgtacgaag atgaggaaga gttctacaat gatatgcaaa ctatcacact gaaatctgag    720

ctgaactag                                                            729


