                         SEQUENCE LISTING

<110>  LORVEN BIOLOGICS PVT. LTD.
 
<120>  PROCESS FOR PREPARATION OF LIRAGLUTIDE USING RECOMBINANT 
       SACCHAROMYCES CEREVISIAE

<130>  IP40548

<160>  10    

<170>  PatentIn version 3.5

<210>  1
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic Acid encoding liraglutide precursor peptide

<400>  1
catgctgaag gtacttttac ttctgatgtt tcttcttatt tggaaggtca agctgctaaa       60

gaatttattg cttggttggt tagaggtaga ggt                                    93


<210>  2
<211>  60
<212>  DNA
<213>  Aspergillus niger

<400>  2
atggttgctt ggtggtcttt gtttttgtat ggtttgcaag ttgctgctcc agctttggct       60


<210>  3
<211>  54
<212>  DNA
<213>  Aspergillus awamorii

<400>  3
atgtctttta gatctttgtt ggctttgtct ggtttggttt gttctggttt ggct             54


<210>  4
<211>  48
<212>  DNA
<213>  Kluyveromyces marxianus

<400>  4
atgaaattgg cttattcttt gttgttgcca ttggctggtg tttctgct                    48


<210>  5
<211>  153
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified open reading frame encoding liraglutide precursor 
       peptide fused with alpha-amylase signal peptide of Aspergillus 
       niger

<400>  5
atggttgctt ggtggtcttt gtttttgtat ggtttgcaag ttgctgctcc agctttggct       60

catgctgaag gtacttttac ttctgatgtt tcttcttatt tggaaggtca agctgctaaa      120

gaatttattg cttggttggt tagaggtaga ggt                                   153


<210>  6
<211>  147
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified open reading frame encoding liraglutide precursor 
       peptide fused with glucoamylase signal peptide of Aspergillus 
       awamori

<400>  6
atgtctttta gatctttgtt ggctttgtct ggtttggttt gttctggttt ggctcatgct       60

gaaggtactt ttacttctga tgtttcttct tatttggaag gtcaagctgc taaagaattt      120

attgcttggt tggttagagg tagaggt                                          147


<210>  7
<211>  141
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Modified open reading frame encoding liraglutide precursor 
       peptide fused with inulinase signal peptide of Kluyveromyces 
       maxianus

<400>  7
atgaaattgg cttattcttt gttgttgcca ttggctggtg tttctgctca tgctgaaggt       60

acttttactt ctgatgtttc ttcttatttg gaaggtcaag ctgctaaaga atttattgct      120

tggttggtta gaggtagagg t                                                141


<210>  8
<211>  51
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of liraglutide precursor peptide fused with 
       alpha-amylase signal peptide of Aspergillus niger

<400>  8

Met Val Ala Trp Trp Ser Leu Phe Leu Tyr Gly Leu Gln Val Ala Ala 
1               5                   10                  15      


Pro Ala Leu Ala His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser 
            20                  25                  30          


Tyr Leu Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg 
        35                  40                  45              


Gly Arg Gly 
    50      


<210>  9
<211>  49
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of liraglutide precursor peptide fused with 
       glucoamylase signal peptide of Aspergillus awamori

<400>  9

Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Ser Gly 
1               5                   10                  15      


Leu Ala His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu 
            20                  25                  30          


Glu Gly Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg 
        35                  40                  45              


Gly 
    


<210>  10
<211>  47
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Polypeptide sequence of liraglutide precursor peptide fused with 
       inulinase signal peptide of Kluyveromyces maxianus

<400>  10

Met Lys Leu Ala Tyr Ser Leu Leu Leu Pro Leu Ala Gly Val Ser Ala 
1               5                   10                  15      


His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
            20                  25                  30          


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Arg Gly Arg Gly 
        35                  40                  45          


