                               SEQUENCE LISTING

<110> PFENEX INC.
 
<120> BACTERIAL LEADER SEQUENCES FOR PERIPLASMIC PROTEIN EXPRESSION

<130> 38194-752.601

<140>
<141>

<150> 62/578,304
<151> 2017-10-27

<160> 20    

<170> PatentIn version 3.5

<210> 1
<211> 25
<212> PRT
<213> Pseudomonas fluorescens

<400> 1
Met Lys Ser Ala Leu Lys Asn Val Ile Pro Gly Ala Leu Ala Leu Leu 
1               5                   10                  15      


Leu Leu Phe Pro Val Ala Ala Gln Ala 
            20                  25  


<210> 2
<211> 18
<212> PRT
<213> Pseudomonas fluorescens

<400> 2
Met Arg Gln Leu Phe Phe Cys Leu Met Leu Met Val Ser Leu Thr Ala 
1               5                   10                  15      


His Ala 
        


<210> 3
<211> 29
<212> PRT
<213> Pseudomonas fluorescens

<400> 3
Met Gln Ser Leu Pro Phe Ser Ala Leu Arg Leu Leu Gly Val Leu Ala 
1               5                   10                  15      


Val Met Val Cys Val Leu Leu Thr Thr Pro Ala Arg Ala 
            20                  25                  


<210> 4
<211> 75
<212> DNA
<213> Pseudomonas fluorescens

<400> 4
atgaaatctg cattgaagaa cgttattccg ggcgccctgg cccttctgct gctattcccc       60

gtcgccgccc aggcc                                                        75


<210> 5
<211> 54
<212> DNA
<213> Pseudomonas fluorescens

<400> 5
atgcgacaac tatttttctg tttgatgctg atggtgtcgc tcacggcgca cgcc             54


<210> 6
<211> 87
<212> DNA
<213> Pseudomonas fluorescens

<400> 6
atgcaaagcc tgccgttctc tgcgttacgc ctgctcggtg tgctggcagt catggtctgc       60

gtgctgttga cgacgccagc ccgtgcc                                           87


<210> 7
<211> 327
<212> PRT
<213> Erwinia chrysanthemi 

<400> 7
Ala Asp Lys Leu Pro Asn Ile Val Ile Leu Ala Thr Gly Gly Thr Ile 
1               5                   10                  15      


Ala Gly Ser Ala Ala Thr Gly Thr Gln Thr Thr Gly Tyr Lys Ala Gly 
            20                  25                  30          


Ala Leu Gly Val Asp Thr Leu Ile Asn Ala Val Pro Glu Val Lys Lys 
        35                  40                  45              


Leu Ala Asn Val Lys Gly Glu Gln Phe Ser Asn Met Ala Ser Glu Asn 
    50                  55                  60                  


Met Thr Gly Asp Val Val Leu Lys Leu Ser Gln Arg Val Asn Glu Leu 
65                  70                  75                  80  


Leu Ala Arg Asp Asp Val Asp Gly Val Val Ile Thr His Gly Thr Asp 
                85                  90                  95      


Thr Val Glu Glu Ser Ala Tyr Phe Leu His Leu Thr Val Lys Ser Asp 
            100                 105                 110         


Lys Pro Val Val Phe Val Ala Ala Met Arg Pro Ala Thr Ala Ile Ser 
        115                 120                 125             


Ala Asp Gly Pro Met Asn Leu Leu Glu Ala Val Arg Val Ala Gly Asp 
    130                 135                 140                 


Lys Gln Ser Arg Gly Arg Gly Val Met Val Val Leu Asn Asp Arg Ile 
145                 150                 155                 160 


Gly Ser Ala Arg Tyr Ile Thr Lys Thr Asn Ala Ser Thr Leu Asp Thr 
                165                 170                 175     


Phe Lys Ala Asn Glu Glu Gly Tyr Leu Gly Val Ile Ile Gly Asn Arg 
            180                 185                 190         


Ile Tyr Tyr Gln Asn Arg Ile Asp Lys Leu His Thr Thr Arg Ser Val 
        195                 200                 205             


Phe Asp Val Arg Gly Leu Thr Ser Leu Pro Lys Val Asp Ile Leu Tyr 
    210                 215                 220                 


Gly Tyr Gln Asp Asp Pro Glu Tyr Leu Tyr Asp Ala Ala Ile Gln His 
225                 230                 235                 240 


Gly Val Lys Gly Ile Val Tyr Ala Gly Met Gly Ala Gly Ser Val Ser 
                245                 250                 255     


Val Arg Gly Ile Ala Gly Met Arg Lys Ala Met Glu Lys Gly Val Val 
            260                 265                 270         


Val Ile Arg Ser Thr Arg Thr Gly Asn Gly Ile Val Pro Pro Asp Glu 
        275                 280                 285             


Glu Leu Pro Gly Leu Val Ser Asp Ser Leu Asn Pro Ala His Ala Arg 
    290                 295                 300                 


Ile Leu Leu Met Leu Ala Leu Thr Arg Thr Ser Asp Pro Lys Val Ile 
305                 310                 315                 320 


Gln Glu Tyr Phe His Thr Tyr 
                325         


<210> 8
<211> 981
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 8
gcagacaaac tccctaacat cgtaatcctc gcaactggtg gtaccatcgc aggcagcgcc       60

gccaccggca cgcagaccac tggctacaag gccggcgcgc tgggcgtaga cacgctgatc      120

aacgccgtcc cggaagtgaa gaaactggcc aacgtcaagg gtgagcaatt ctccaacatg      180

gccagcgaga acatgactgg cgatgtggta ctgaagctct cgcagcgcgt gaacgaactg      240

ctcgcccgcg acgacgtgga cggcgtggtg atcacccacg gcactgatac cgtcgaagag      300

tcggcgtact ttctccacct gaccgtgaag tccgataagc ccgtggtgtt tgtcgccgcg      360

atgcgcccgg cgaccgccat cagcgccgac gggccgatga atctgttgga agccgtgcgc      420

gtggcgggtg acaagcaaag ccgcggtcgg ggcgtaatgg tcgtcctgaa cgatcggatc      480

ggtagcgcgc ggtacatcac caagacgaac gcctccacgc tggacacctt caaggcgaac      540

gaagaggggt acctgggggt gatcattggc aatcgtatct attaccagaa ccgcatcgac      600

aagctgcaca ccacccgctc ggtgttcgac gtgcgcggtc tgactagcct gcccaaggtc      660

gacatcctgt acggctacca agacgacccg gagtacctct acgacgcggc gatccagcat      720

ggcgtgaagg gcatcgtcta cgccggtatg ggtgccggct cggtgtcggt ccgcggcatc      780

gcgggtatgc gcaaggccat ggagaaaggc gtggtcgtga ttcgctcgac ccggactggc      840

aatggcatcg taccgcccga tgaagaactc ccggggctcg tgagcgatag cctcaacccc      900

gcgcacgccc ggatcctgct gatgctggcg ctcacgcgga ccagcgaccc caaggtcatt      960

caagagtact tccacaccta c                                                981


<210> 9
<211> 326
<212> PRT
<213> Escherichia coli

<400> 9
Leu Pro Asn Ile Thr Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Gly 
1               5                   10                  15      


Gly Asp Ser Ala Thr Lys Ser Asn Tyr Thr Ala Gly Lys Val Gly Val 
            20                  25                  30          


Glu Asn Leu Val Asn Ala Val Pro Gln Leu Lys Asp Ile Ala Asn Val 
        35                  40                  45              


Lys Gly Glu Gln Val Val Asn Ile Gly Ser Gln Asp Met Asn Asp Asp 
    50                  55                  60                  


Val Trp Leu Thr Leu Ala Lys Lys Ile Asn Thr Asp Cys Asp Lys Thr 
65                  70                  75                  80  


Asp Gly Phe Val Ile Thr His Gly Thr Asp Thr Met Glu Glu Thr Ala 
                85                  90                  95      


Tyr Phe Leu Asp Leu Thr Val Lys Cys Asp Lys Pro Val Val Met Val 
            100                 105                 110         


Gly Ala Met Arg Pro Ser Thr Ser Met Ser Ala Asp Gly Pro Phe Asn 
        115                 120                 125             


Leu Tyr Asn Ala Val Val Thr Ala Ala Asp Lys Ala Ser Ala Asn Arg 
    130                 135                 140                 


Gly Val Leu Val Val Met Asn Asp Thr Val Leu Asp Gly Arg Asp Val 
145                 150                 155                 160 


Thr Lys Thr Asn Thr Thr Asp Val Ala Thr Phe Lys Ser Val Asn Tyr 
                165                 170                 175     


Gly Pro Leu Gly Tyr Ile His Asn Gly Lys Ile Asp Tyr Gln Arg Thr 
            180                 185                 190         


Pro Ala Arg Lys His Thr Ser Asp Thr Pro Phe Asp Val Ser Lys Leu 
        195                 200                 205             


Asn Glu Leu Pro Lys Val Gly Ile Val Tyr Asn Tyr Ala Asn Ala Ser 
    210                 215                 220                 


Asp Leu Pro Ala Lys Ala Leu Val Asp Ala Gly Tyr Asp Gly Ile Val 
225                 230                 235                 240 


Ser Ala Gly Val Gly Asn Gly Asn Leu Tyr Lys Thr Val Phe Asp Thr 
                245                 250                 255     


Leu Ala Thr Ala Ala Lys Asn Gly Thr Ala Val Val Arg Ser Ser Arg 
            260                 265                 270         


Val Pro Thr Gly Ala Thr Thr Gln Asp Ala Glu Val Asp Asp Ala Lys 
        275                 280                 285             


Tyr Gly Phe Val Ala Ser Gly Thr Leu Asn Pro Gln Lys Ala Arg Val 
    290                 295                 300                 


Leu Leu Gln Leu Ala Leu Thr Gln Thr Lys Asp Pro Gln Gln Ile Gln 
305                 310                 315                 320 


Gln Ile Phe Asn Gln Tyr 
                325     


<210> 10
<211> 978
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 10
ctccctaaca ttactattct ggccactggc ggtacgattg caggcggcgg tgactcagcc       60

accaagtcga attacaccgc cggtaaggtc ggtgtcgaaa acctcgtcaa cgccgtgccg      120

cagctgaaag atatcgccaa cgtcaagggc gagcaagtgg tgaacatcgg ctcccaagat      180

atgaacgatg acgtgtggct gacgctggcc aagaaaatca acaccgattg cgacaagacg      240

gacgggtttg tcatcaccca cggcaccgac actatggaag agactgccta cttcctcgac      300

ctcacggtga agtgcgataa accggtagtg atggtgggcg ccatgcgccc gagcacctcg      360

atgagcgcgg acggcccgtt caatctgtac aacgccgtgg taaccgcagc ggacaaggcg      420

tccgcgaacc gcggtgtatt ggtagtgatg aacgatacgg tgctcgatgg gcgcgatgtg      480

accaagacca ataccactga tgtggccacc ttcaagagcg tgaactatgg cccgctgggc      540

tacatccata acggcaagat cgattaccag cgtactcccg cccggaagca cacctcggac      600

acccccttcg acgtgtcgaa actgaacgaa ctgcccaagg tcggcatcgt ctacaactac      660

gccaatgcga gcgatctgcc cgcgaaggcc ctggtggacg ccggctacga cgggatcgta      720

tcggcgggtg tgggcaatgg taacctgtac aagaccgtgt ttgacaccct ggcgacggcg      780

gcgaagaacg gcaccgccgt ggtccgcagc agccgcgtgc ccactggggc gaccacccaa      840

gacgccgagg tcgacgacgc gaagtacggc ttcgtagcca gcggcaccct gaacccgcaa      900

aaggcccggg tcctgctgca gctggcgctc acgcagacga aggacccgca gcaaatccaa      960

cagatcttca accagtac                                                    978


<210> 11
<211> 6
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
aggagg                                                                   6


<210> 12
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS2 sequence

<400> 12
ggagcg                                                                   6


<210> 13
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS34 sequence

<400> 13
ggagcg                                                                   6


<210> 14
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS41 sequence

<400> 14
aggagt                                                                   6


<210> 15
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS43 sequence

<400> 15
ggagtg                                                                   6


<210> 16
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS48 sequence

<400> 16
gagtaa                                                                   6


<210> 17
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS1 sequence

<400> 17
agagag                                                                   6


<210> 18
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS35 sequence

<400> 18
aaggca                                                                   6


<210> 19
<211> 6
<212> DNA
<213> Unknown

<220>
<223> Description of Unknown: 
      RBS49 sequence

<400> 19
ccgaac                                                                   6


<210> 20
<211> 1020
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<400> 20
atatgctctt cagccgcaga caaactccct aacatcgtaa tcctcgcaac tggtggtacc       60

atcgcaggca gcgccgccac cggcacgcag accactggct acaaggccgg cgcgctgggc      120

gtagacacgc tgatcaacgc cgtcccggaa gtgaagaaac tggccaacgt caagggtgag      180

caattctcca acatggccag cgagaacatg actggcgatg tggtactgaa gctctcgcag      240

cgcgtgaacg aactgctcgc ccgcgacgac gtggacggcg tggtgatcac ccacggcact      300

gataccgtcg aagagtcggc gtactttctc cacctgaccg tgaagtccga taagcccgtg      360

gtgtttgtcg ccgcgatgcg cccggcgacc gccatcagcg ccgacgggcc gatgaatctg      420

ttggaagccg tgcgcgtggc gggtgacaag caaagccgcg gtcggggcgt aatggtcgtc      480

ctgaacgatc ggatcggtag cgcgcggtac atcaccaaga cgaacgcctc cacgctggac      540

accttcaagg cgaacgaaga ggggtacctg ggggtgatca ttggcaatcg tatctattac      600

cagaaccgca tcgacaagct gcacaccacc cgctcggtgt tcgacgtgcg cggtctgact      660

agcctgccca aggtcgacat cctgtacggc taccaagacg acccggagta cctctacgac      720

gcggcgatcc agcatggcgt gaagggcatc gtctacgccg gtatgggtgc cggctcggtg      780

tcggtccgcg gcatcgcggg tatgcgcaag gccatggaga aaggcgtggt cgtgattcgc      840

tcgacccgga ctggcaatgg catcgtaccg cccgatgaag aactcccggg gctcgtgagc      900

gatagcctca accccgcgca cgcccggatc ctgctgatgc tggcgctcac gcggaccagc      960

gaccccaagg tcattcaaga gtacttccac acctactgat aatagttcag aagagcatat     1020



