﻿                         序列表

<110>  北京中科伊品生物科技有限公司
       中国科学院微生物研究所

<120>  生产L-赖氨酸的重组菌、其构建方法以及L-赖氨酸的生产方法

<130>  170710CI

<141>  2017-11-01

<160>  57

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  1638
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  1
ctgcgacagc atggaactca gtgcaatggc tgtaaggcct gcaccaacaa tgattgagcg        60
aagctccaaa atgtcctccc cgggttgata ttagatttca taaatatact aaaaatcttg       120
agagtttttc cgttgaaaac taaaaagctg ggaaggtgaa tcgaatttcg gggctttaaa       180
gcaaaaatga acagcttggt ctatagtggc taggtaccct ttttgttttg gacacatgta       240
gggtggccga aacaaagtaa taggacaaca acgctcgacc gcgattattt ttggagaatc       300
atgacctcag catctgcccc aagctttaac cccggcaagg gtcccggctc agcagtcgga       360
attgcccttt taggattcgg aacagtcggc actgaggtga tgcgtctgat gaccgagtac       420
ggtgatgaac ttgcgcaccg cattggtggc ccactggagg ttcgtggcat tgctgcttct       480
gatatctcaa agccacgtga aggcgttgca cctgagctgc tcactgagga cgcttttgca       540
ctcatcgagc gcgaggatgt tgacatcgtc gttgaggtta tcggcggcat tgagtaccca       600
cgtgaggtag ttctcgcagc tctgaaggcc ggcaagtctg ttgttaccgc caataaggct       660
cttgttgcag ctcactctgc tgagcttgct gatgcagcgg aagccgcaaa cgttgacctg       720
tacttcgagg ctgctgttgc aggcgcaatt ccagtggttg gcccactgcg tcgctccctg       780
gctggcgatc agatccagtc tgtgatgggc atcgttaacg gcaccaccaa cttcatcttg       840
gacgccatgg attccaccgg cgctgactat gcagattctt tggctgaggc aactcgtttg       900
ggttacgccg aagctgatcc aactgcagac gtcgaaggcc atgacgccgc atccaaggct       960
gcaattttgg catccatcgc tttccacacc cgtgttaccg cggatgatgt gtactgcgaa      1020
ggtatcagca acatcagcgc tgccgacatt gaggcagcac agcaggcagg ccacaccatc      1080
aagttgttgg ccatctgtga gaagttcacc aacaaggaag gaaagtcggc tatttctgct      1140
cgcgtgcacc cgactctatt acctgtgtcc cacccactgg cgtcggtaaa caagtccttt      1200
aatgcaatct ttgttgaagc agaagcagct ggtcgcctga tgttctacgg aaacggtgca      1260
ggtggcgcgc caaccgcgtc tgctgtgctt ggcgacgtcg ttggtgccgc acgaaacaag      1320
gtgcacggtg gccgtgctcc aggtgagtcc acctacgcta acctgccgat cgctgatttc      1380
ggtgagacca ccactcgtta ccacctcgac atggatgtgg aagatcgcgt gggggttttg      1440
gctgaattgg ctagcctgtt ctctgagcaa ggaatctccc tgcgtacaat ccgacaggaa      1500
gagcgcgatg atgatgcacg tctgatcgtg gtcacccact ctgcgctgga atctgatctt      1560
tcccgcaccg ttgaactgct gaaggctaag cctgttgtta aggcaatcaa cagtgtgatc      1620
cgcctcgaaa gggactaa                                                    1638

<210>  2
<211>  26
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(26)
<223>  hom-V59A上游正向引物


<400>  2
gctctagaag ctgtttcaca atttct                                             26

<210>  3
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(19)
<223>  hom-V59A突变位点反向引物


<400>  3
atatcagaag cagcaatgc                                                     19

<210>  4
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(19)
<223>  hom-V59A突变位点正向引物


<400>  4
gcattgctgc ttctgatat                                                     19

<210>  5
<211>  27
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(27)
<223>  hom-V59A下游反向引物


<400>  5
ccggaattcc caacaacttg atggtgt                                            27

<210>  6
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(19)
<223>  hom-V59A正向鉴定引物


<400>  6
tctacgttgt atctcgcac                                                     19

<210>  7
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(18)
<223>  hom-V59A反向鉴定引物


<400>  7
caggcgacca gctgcttc                                                      18

<210>  8
<211>  2280
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  8
aaaaccgatg tttgattggg ggaatcgggg gttacgatac taggacgcag tgactgctat        60
cacccttggc ggtctcttgt tgaaaggaat aattactcta gtgtcgactc acacatcttc       120
aacgcttcca gcattcaaaa agatcttggt agcaaaccgc ggcgaaatcg cggtccgtgc       180
tttccgtgca gcactcgaaa ccggtgcagc cacggtagct atttaccccc gtgaagatcg       240
gggatcattc caccgctctt ttgcttctga agctgtccgc attggtaccg aaggctcacc       300
agtcaaggcg tacctggaca tcgatgaaat tatcggtgca gctaaaaaag ttaaagcaga       360
tgccatttac ccgggatacg gcttcctgtc tgaaaatgcc cagcttgccc gcgagtgtgc       420
ggaaaacggc attactttta ttggcccaac cccagaggtt cttgatctca ccggtgataa       480
gtctcgcgcg gtaaccgccg cgaagaaggc tggtctgcca gttttggcgg aatccacccc       540
gagcaaaaac atcgatgaga tcgttaaaag cgctgaaggc cagacttacc ccatctttgt       600
gaaggcagtt gccggtggtg gcggacgcgg tatgcgtttt gttgcttcac ctgatgagct       660
tcgcaaatta gcaacagaag catctcgtga agctgaagcg gctttcggcg atggcgcggt       720
atatgtcgaa cgtgctgtga ttaaccctca gcatattgaa gtgcagatcc ttggcgatca       780
cactggagaa gttgtacacc tttatgaacg tgactgctca ctgcagcgtc gtcaccaaaa       840
agttgtcgaa attgcgccag cacagcattt ggatccagaa ctgcgtgatc gcatttgtgc       900
ggatgcagta aagttctgcc gctccattgg ttaccagggc gcgggaaccg tggaattctt       960
ggtcgatgaa aagggcaacc acgtcttcat cgaaatgaac ccacgtatcc aggttgagca      1020
caccgtgact gaagaagtca ccgaggtgga cctggtgaag gcgcagatgc gcttggctgc      1080
tggtgcaacc ttgaaggaat tgggtctgac ccaagataag atcaagaccc acggtgcagc      1140
actgcagtgc cgcatcacca cggaagatcc aaacaacggc ttccgcccag ataccggaac      1200
tatcaccgcg taccgctcac caggcggagc tggcgttcgt cttgacggtg cagctcagct      1260
cggtggcgaa atcaccgcac actttgactc catgctggtg aaaatgacct gccgtggttc      1320
cgactttgaa actgctgttg ctcgtgcaca gcgcgcgttg gctgagttca ccgtgtctgg      1380
tgttgcaacc aacattggtt tcttgcgtgc gttgctgcgg gaagaggact tcacttccaa      1440
gcgcatcgcc accggattca ttgccgatca ctcgcacctc cttcaggctc cacctgctga      1500
tgatgagcag ggacgcatcc tggattactt ggcagatgtc accgtgaaca agcctcatgg      1560
tgtgcgtcca aaggatgttg cagctcctat cgataagctg cctaacatca aggatctgcc      1620
actgccacgc ggttcccgtg accgcctgaa gcagcttggc ccagccgcgt ttgctcgtga      1680
tctccgtgag caggacgcac tggcagttac tgataccacc ttccgcgatg cacaccagtc      1740
tttgcttgcg acccgagtcc gctcattcgc actgaagcct gcggcagagg ccgtcgcaaa      1800
gctgactcct gagcttttgt ccgtggaggc ctggggcggc gcgacctacg atgtggcgat      1860
gcgtttcctc tttgaggatc cgtgggacag gctcgacgag ctgcgcgagg cgatgccgaa      1920
tgtaaacatt cagatgctgc ttcgcggccg caacaccgtg ggatacaccc cgtacccaga      1980
ctccgtctgc cgcgcgtttg ttaaggaagc tgccagctcc ggcgtggaca tcttccgcat      2040
cttcgacgcg cttaacgacg tctcccagat gcgtccagca atcgacgcag tcctggagac      2100
caacaccgcg gtagccgagg tggctatggc ttattctggt gatctctctg atccaaatga      2160
aaagctctac accctggatt actacctaaa gatggcagag gagatcgtca agtctggcgc      2220
tcacatcttg gccattaagg atatggctgg tctgcttcgc ccagctgcgg taaccaagct      2280

<210>  9
<211>  28
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(28)
<223>  pyc-P458S上游正向引物


<400>  9
cccaagcttt gactgctcac tgcagcgt                                           28

<210>  10
<211>  17
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(17)
<223>  pyc-P458S突变位点反向引物


<400>  10
aggtgcgagt gatcggc                                                       17

<210>  11
<211>  17
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(17)
<223>  pyc-P458S突变位点正向引物


<400>  11
gccgatcact cgcacct                                                       17

<210>  12
<211>  26
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(26)
<223>  pyc-P458S下游反向引物


<400>  12
gctctagagc gtcgattgct ggacgc                                             26

<210>  13
<211>  19
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(19)
<223>  pyc-P458S正向鉴定引物


<400>  13
cgcaaattag caacagaag                                                     19

<210>  14
<211>  18
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(18)
<223>  pyc-P458S反向鉴定引物


<400>  14
ccttaatggc caagatgt                                                      18

<210>  15
<211>  750
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  15
acctggccct cgatacctcg agtctggtgg cggctttgtc tgaagatatt tctggcgccg        60
gattaaatga cctgaaagtt ctcgacgtcg gcggcggacc cggatacttc gccgaagcct       120
ttgagacact gggcgccacc tacttctccg tcgaacccga cgttggcgaa atgtccgcag       180
ctggcatcga cgtccacgga tcagtccgcg gatccggcct cgacctgccg tttcttcccg       240
attcctttga cgtggtgtac tcctccaacg ttgcagaaca tgtctccgca ccgtgggaat       300
tgggagaaga aatgctccgc gtcacccgca gcggcggcct ggcaatcctg agctacacca       360
tttggttagg gcccttcggc ggccatgaaa ccggactgtg ggaacactac gttggcggag       420
aatttgcccg cgatcgctac acgaagaaac acgggcaccc gcctaagaac gttttcgggg       480
agtcactgtt taatgtgtcc tgccgggagg ggctggaatg gggagcctcc gtgggcaatg       540
cggaattggt tgccgctttt ccccgctacc acccgtattg ggtctggtgg atggttaaag       600
tcccagtgct ccgagaattc gcggtaagta acttggtgtt ggtgtttaaa aagcactgag       660
gttttgagga attcatcgct taacgacaag aaaggctccc actttcggtg ggagcctttc       720
ttgttattta gcagttctta agcgtgaact                                        750

<210>  16
<211>  767
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  16
tacttctcca gattttgtgt cattcgacag agttctcgcc ccctagcgta gctttcagat        60
acagaactag ttaaaacttt aggtgagaca acggacacat ttgtcattac cagtggacct       120
accccctgcc cacacgcatc tacacacttt ctttaatatg agagcacccg tttaaatagc       180
ctattttggg ggtggtttca agaattaacc tcaaccgttc tccgacagtt cattccccgt       240
ccatggccat tgggttcaga tttgggcaat tctcacacat tccaggggac aactttccca       300
gttttcccac cattaacact taacattcgg acaataggca acaaaacgcc aagaacagcg       360
gtaatggtaa tccctttccc ttaccctgcc atcacaatcc aagcactccg ctagtggccg       420
accagcacaa accggcccac tgtcagtaca caccttttta aaacaacatt tacactcaca       480
tgcatgcccg cactgtcacc acccgccctc aactaccgaa ctaaagatat gtacttgaag       540
ccaaattttt accctagatc ccccttttaa atactttgaa aattactcac acacatcccc       600
acgttacccc aaaggttata tccagttagt cgtatcaaaa agtgctctga tcttaacttt       660
gccctaccta aatacatgac cccaccacga cggccagtac taacgacaga atccactagc       720
gaacccattt attaacaaac attgcaaaca agtgttgaag tattcgc                     767

<210>  17
<211>  960
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  17
gctcctttta aaaaattcca cccgctgctg aaatgagcct ttacaggcct aggtaatgct        60
caagtcctac gactaggcct gggtgctgtg caatgttgcg cacacccacc aagacacctg       120
gtgcaaatga gttgcgatca taggagtcct gcttgatggt caaggtctga ccctgggtgc       180
caaagataac ttgctcgtga gcaaccatgc cggacatgcg gactgcatga accgggattc       240
catctacgct tgcgccacgg gaaccctcaa gtgcctgctc ggtcgcatct ggctgtgcgt       300
ccatgcctgc ttctttgcgt gccgcagcaa tgccctgagc agtgtggatc gcggtgcctg       360
aaggtgcatc cagcttgttg gggtggtgca gctcaataac ttcagctgat tcgaagaagc       420
gggcagcctg cttggaaaag accatggtca acaccgcaga gatagcaaag ttaggtgcga       480
tcagaacacc gacattgtct tttccttcaa gccagtcgcg aacctgctcc aaacgagcat       540
catcgaagcc cgtggttcca acaaccgcag aaatgccgtt gttgatgcag aactccaggt       600
tgcccatcac agcgttagga gtggtgaagt caacgacaac ttcagcgccg ttgtctacca       660
gaaggctcaa atcatcgtcg acgccgatct ctgcaacaag ctccagatcg tcggactcat       720
tgactgctgc cacaatagtt tgaccaacac ggcctttggc tccgagaacg ccaaccttga       780
ttcccattat gctccttcat tttcgtgggg cgaagagttt ttcaaacgcc caccagtcta       840
gacgtacccg ttcagaaggt gctgatatcc atacctaact gaccgttttg gtctgtgttg       900
ttaacgattg ttcatcagtt tgttccgttc tagggattaa tcacacaggt ggaactatgt       960

<210>  18
<211>  49
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(49)
<223>  pck上游同源臂正向引物


<400>  18
tctagagtcg acctgcaggc atgcaagctt acctggccct cgatacctc                    49

<210>  19
<211>  41
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(41)
<223>  pck上游同源臂反向引物


<400>  19
cctaggcctg taaagttcac gcttaagaac tgctaaataa c                            41

<210>  20
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  pyc启动子正向引物


<400>  20
tgtgagtcga cattagagta attattcctt tcaacaagag                              40

<210>  21
<211>  34
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(34)
<223>  pyc启动子反向引物


<400>  21
atctggagaa gtatgcgtta aacttggcca aatg                                    34

<210>  22
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  pyc*正向引物


<400>  22
tccgttctag ggattaggaa acgacgacga tc                                      32

<210>  23
<211>  36
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(36)
<223>  pyc*反向引物


<400>  23
aggaataatt actctaatgt cgactcacac atcttc                                  36

<210>  24
<211>  31
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(31)
<223>  dapB正向引物


<400>  24
ttaagcgtga actttacagg cctaggtaat g                                       31

<210>  25
<211>  36
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(36)
<223>  dapB反向引物


<400>  25
tcgtcgtcgt ttcctaatcc ctagaacgga acaaac                                  36

<210>  26
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  pck下游同源臂正向引物


<400>  26
caagtttaac gcatacttct ccagattttg tg                                      32

<210>  27
<211>  49
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(49)
<223>  pck下游同源臂反向引物


<400>  27
cgttgtaaaa cgacggccag tgccaagctt gcgaatactt caacacttg                    49

<210>  28
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  pyc*-dapB鉴定正向引物


<400>  28
taccttgggc aggtcgtggg                                                    20

<210>  29
<211>  20
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(20)
<223>  pyc*-dapB鉴定反向引物


<400>  29
tgggagcgtt gtgcgctcga                                                    20

<210>  30
<211>  1266
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  30
atggccctgg tcgtacagaa atatggcggt tcctcgcttg agagtgcgga acgcattaga        60
aacgtcgctg aacggatcgt tgccaccaag aaggctggaa atgatgtcgt ggttgtctgc       120
tccgcaatgg gagacaccac ggatgaactt ctagaacttg cagcggcagt gaatcccgtt       180
ccgccagctc gtgaaatgga tatgctcctg actgctggtg agcgtatttc taacgctctc       240
gtcgccatgg ctattgagtc ccttggcgca gaagcccaat ctttcacggg ctctcaggct       300
ggtgtgctca ccaccgagcg ccacggaaac gcacgcattg ttgatgtcac tccaggtcgt       360
gtgcgtgaag cactcgatga gggcaagatc tgcattgttg ctggtttcca gggtgttaat       420
aaagaaaccc gcgatgtcac cacgttgggt cgtggtggtt ctgacaccac tgcagttgcg       480
ttggcagctg ctttgaacgc tgatgtgtgt gagatttact cggacgttga cggtgtgtat       540
accgctgacc cgcgcatcgt tcctaatgca cagaagctgg aaaagctcag cttcgaagaa       600
atgctggaac ttgctgctgt tggctccaag attttggtgc tgcgcagtgt tgaatacgct       660
cgtgcattca atgtgccact tcgcgtacgc tcgtcttata gtaatgatcc cggcactttg       720
attgccggct ctatggagga tattcctgtg gaagaagcag tccttaccgg tgtcgcaacc       780
gacaagtccg aagccaaagt aaccgttctg ggtatttccg ataagccagg cgaggctgcg       840
aaggttttcc gtgcgttggc tgatgcagaa atcaacattg acatggttct gcagaacgtc       900
tcttctgtag aagacggcac caccgacatc atcttcacct gccctcgttc cgacggccgc       960
cgcgcgatgg agatcttgaa gaagcttcag gttcagggca actggaccaa tgtgctttac      1020
gacgaccagg tcggcaaagt ctccctcgtg ggtgctggca tgaagtctca cccaggtgtt      1080
accgcagagt tcatggaagc tctgcgcgat gtcaacgtga acatcgaatt gatttccacc      1140
tctgagattc gtatttccgt gctgatccgt gaagatgatc tggatgctgc tgcacgtgca      1200
ttgcatgagc agttccagct gggcggcgaa gacgaagccg tcgtttatgc aggcaccgga      1260
cgctaa                                                                 1266

<210>  31
<211>  963
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  31
atgaccaaca tccgcgtagc tatcgtgggc tacggaaacc tgggacgcag cgtcgaaaag        60
cttattgcca agcagcccga catggacctt gtaggaatct tctcgcgccg ggccaccctc       120
gacacaaaga cgccagtctt tgatgtcgcc gacgtggaca agcacgccga cgacgtggac       180
gtgctgttcc tgtgcatggg ctccgccacc gacatccctg agcaggcacc aaagttcgcg       240
cagttcgcct gcaccgtaga cacctacgac aaccaccgcg acatcccacg ccaccgccag       300
gtcatgaacg aagccgccac cgcagccggc aacgttgcac tggtctctac cggctgggat       360
ccaggaatgt tctccatcaa ccgcgtctac gcagcggcag tcttagccga gcaccagcag       420
cacaccttct ggggcccagg tttgtcacag ggccactccg atgctttgcg acgcatccct       480
ggcgttcaaa aggcagtcca gtacaccctc ccatccgaag acgccctgga aaaggcccgc       540
cgcggcgaag ccggcgacct taccggaaag caaacccaca agcgccaatg cttcgtggtt       600
gccgacgcgg ccgatcacga gcgcatcgaa aacgacatcc gcaccatgcc tgattacttc       660
gttggctacg aagtcgaagt caacttcatc gacgaagcaa ccttcgactc cgagcacacc       720
ggcatgccac acggtggcca cgtgattacc accggcgaca ccggtggctt caaccacacc       780
gtggaataca tcctcaagct ggaccgaaac ccagatttca ccgcttcctc acagatcgct       840
ttcggtcgcg cagctcaccg catgaagcag cagggccaaa gcggagcttt caccgtcctc       900
gaagttgctc catacctgct ctccccagag aacttggacg atctgatcgc acgcgacgtc       960
taa                                                                     963

<210>  32
<211>  1338
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  32
atggctacag ttgaaaattt caatgaactt cccgcacacg tatggccacg caatgccgtg        60
cgccaagaag acggcgttgt caccgtcgct ggtgtgcctc tgcctgacct cgctgaagaa       120
tacggaaccc cactgttcgt agtcgacgag gacgatttcc gttcccgctg tcgcgacatg       180
gctaccgcat tcggtggacc aggcaatgtg cactacgcat ctaaagcgtt cctgaccaag       240
accattgcac gttgggttga tgaagagggg ctggcactgg acattgcatc catcaacgaa       300
ctgggcattg ccctggccgc tggtttcccc gccagccgta tcaccgcgca cggcaacaac       360
aaaggcgtag agttcctgcg cgcgttggtt caaaacggtg tgggacacgt ggtgctggac       420
tccgcacagg aactagaact gttggattac gttgccgctg gtgaaggcaa gattcaggac       480
gtgttgatcc gcgtaaagcc aggcatcgaa gcacacaccc acgagttcat cgccactagc       540
cacgaagacc agaagttcgg attctccctg gcatccggtt ccgcattcga agcagcaaaa       600
gccgccaaca acgcagaaaa cctgaacctg gttggcctgc actgccacgt tggttcccag       660
gtgttcgacg ccgaaggctt caagctggca gcagaacgcg tgttgggcct gtactcacag       720
atccacagcg aactgggcgt tgcccttcct gaactggatc tcggtggcgg atacggcatt       780
gcctataccg cagctgaaga accactcaac gtcgcagaag ttgcctccga cctgctcacc       840
gcagtcggaa aaatggcagc ggaactaggc atcgacgcac caaccgtgct tgttgagccc       900
ggccgcgcta tcgcaggccc ctccaccgtg accatctacg aagtcggcac caccaaagac       960
gtccacgtag acgacgacaa aacccgccgt tacatcgccg tggacggagg catgtccgac      1020
aacatccgcc cagcactcta cggctccgaa tacgacgccc gcgtagtatc ccgcttcgcc      1080
gaaggagacc cagtaagcac ccgcatcgtg ggctcccact gcgaatccgg cgatatcctg      1140
atcaacgatg aaatctaccc atctgacatc accagcggcg acttccttgc actcgcagcc      1200
accggcgcat actgctacgc catgagctcc cgctacaacg ccttcacacg gcccgccgtc      1260
gtgtccgtcc gcgctggcag ctcccgcctc atgctgcgcc gcgaaacgct cgacgacatc      1320
ctctcactag aggcataa                                                    1338

<210>  33
<211>  58
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(58)
<223>  lysC正向引物


<400>  33
caggtcgact ctagaggatc cccgggaaag gaggacaacc atggccctgg tcgtacag          58

<210>  34
<211>  53
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(53)
<223>  lysC反向引物


<400>  34
caccgacatc atcttcacct gcgttgtcct cctttttagc gtccggtgcc tgc               53

<210>  35
<211>  43
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(43)
<223>  ddh正向引物


<400>  35
caccggacgc taaaaaggag gacaaccatg accaacatcc gcg                          43

<210>  36
<211>  32
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(32)
<223>  ddh反向引物


<400>  36
gttgtcctcc tttttagacg tcgcgtgcga tc                                      32

<210>  37
<211>  45
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(45)
<223>  lysA正向引物


<400>  37
acgcgacgtc taaaaaggag gacaaccatg gctacagttg aaaat                        45

<210>  38
<211>  49
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(49)
<223>  lysA反向引物


<400>  38
ctcatccgcc aaaacagcca agctgaattc ttatgcctct agtgagagg                    49

<210>  39
<211>  978
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  39
atgtcgaagc agcactccac accattaaac aatgatgaag aacacacttc cgctcctcaa        60
aaggttgcgg taatcaccac gggcggaacc atcgcctgta cttccgacgc aaatgggcat       120
ctgcttccca ccgtcagcgg tgcagacctg cttgcgccaa tcgcaccacg gttcaatgga       180
gcgcagatcg ctttcgaaat ccacgaaatc aaccgccttg attcctcctc catgacgttt       240
gaggatctcg attccatcat cgccacggtt cataaggtgt tggaggatcc ggatgttgtt       300
ggcgtagtag ttacccacgg caccgattcc atggaagagt ccgccatcgc cgtagacacc       360
ttccttgatg atccccgccc agtcattttc accggcgccc aaaaaccctt cgatcatccc       420
gaagccgacg gcccaaacaa ccttttcgaa gcctgcctca tcgcatccga cccctccgct       480
cgcggaattg gtgcactcat tgtcttcggt cacgccgtca tccctgctcg cggctgcgtt       540
aaatggcaca cctctgatga gctggcgttt gcaaccaacg gccctgaaga accagagcgc       600
cccgatgcgc tgcccgtagc taaattggcg gatgtctctg tcgaaatcat ccccgcatac       660
cctggtgcca ccggcgcaat ggtggaagct gccatcgctg ccggtgctca aggacttgta       720
gtggaagcaa tgggatcagg caatgttggt tcccgcatgg gtgatgccct aggtaaagca       780
cttgacgctg gaattcccgt ggtgatgagc actagggttc ctcgtggtga agtatccgga       840
gtgtatggcg gtgcaggtgg aggtgcgact ttggctgcga agggcgctgt gggatctcgc       900
tacttcagag ctggtcaggc acgtattttg ctcgcgattg ccattgcgac gggcgcacat       960
ccggtgacgc tttactaa                                                     978

<210>  40
<211>  200
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  40
tggccgttac cctgcgaatg tccacagggt agctggtagt ttgaaaatca acgccgttgc        60
ccttaggatt cagtaactgg cacattttgt aatgcgctag atctgtgtgc tcagtcttcc       120
aggctgctta tcacagtgaa agcaaaacca attcgtggct gcgaaagtcg tagccaccac       180
gaagtccagg aggacataca                                                   200

<210>  41
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  插入Ptuf上游同源臂正向引物


<400>  41
ccggaattct gctcaggagc aacagtatt                                          29

<210>  42
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  插入Ptuf上游同源臂反向引物


<400>  42
cattcgcagg gtaacggcca gcgctctagc gtatcaacta                              40

<210>  43
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  Ptuf正向引物


<400>  43
tagttgatac gctagagcgc tggccgttac cctgcgaatg                              40

<210>  44
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  Ptuf反向引物


<400>  44
gtggagtgct gcttcgacat tgtatgtcct cctggacttc                              40

<210>  45
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  插入Ptuf下游同源臂正向引物


<400>  45
gaagtccagg aggacataca atgtcgaagc agcactccac                              40

<210>  46
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  插入Ptuf下游同源臂反向引物


<400>  46
tgctctagac agcgatggca gcttccacc                                          29

<210>  47
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  增加ncgl2026拷贝上游同源臂正向引物


<400>  47
tgctctagaa agggcaatga gtttgtcga                                          29

<210>  48
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  增加ncgl2026拷贝上游同源臂反向引物


<400>  48
gtggagtgct gcttcgacat ttagttctcc aagtagagcc                              40

<210>  49
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  ncgl2026正向引物


<400>  49
ggctctactt ggagaactaa atgtcgaagc agcactccac                              40

<210>  50
<211>  39
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(39)
<223>  ncgl2026反向引物


<400>  50
tatcagacga gatcttggat tagtaaagcg tcaccggat                               39

<210>  51
<211>  39
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(39)
<223>  增加ncgl2026拷贝下游同源臂正向引物


<400>  51
atccggtgac gctttactaa tccaagatct cgtctgata                               39

<210>  52
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  增加ncgl2026拷贝下游同源臂反向引物


<400>  52
ctagctagcg tgtggatccg agcgcgaag                                          29

<210>  53
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  ncgl2026敲除上游同源臂正向引物


<400>  53
ccggaattct gctcaggagc aacagtatt                                          29

<210>  54
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  ncgl2026敲除上游同源臂反向引物


<400>  54
atgcaagacc aagggcgaaa gcgctctagc gtatcaacta                              40

<210>  55
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(40)
<223>  ncgl2026敲除下游同源臂正向引物


<400>  55
tagttgatac gctagagcgc tttcgccctt ggtcttgcat                              40

<210>  56
<211>  29
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<221>  primer_bind
<222>  (1)..(29)
<223>  ncgl2026敲除下游同源臂反向引物


<400>  56
ctagctagct tatgaggtag gcgtgcaat                                          29

<210>  57
<211>  244
<212>  DNA
<213>  谷氨酸棒状杆菌 (Corynebacterium glutamicum)

<400>  57
tgcgttaaac ttggccaaat gtggcaacct ttgcaaggtg aaaaactggg gcggggttag        60
atcctggggg gtttatttca ttcactttgg cttgaagtcg tgcaggtcag gggagtgttg       120
cccgaaaaca ttgagaggaa aacaaaaacc gatgtttgat tgggggaatc gtgtggtata       180
atggtaggac gcagtgactg ctatcaccct tggcggtctc ttgttgaaag gaataattac       240
tcta                                                                    244

