                         SEQUENCE LISTING

<110>  The University of North Carolina at Chapel Hill
       Gray, Steven James
 
<120>  OPTIMIZED AGA GENES AND EXPRESSION CASSETTES AND THEIR USE

<130>  5470-805WO

<150>  US 62/582,664
<151>  2017-11-07

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  1044
<212>  DNA
<213>  Artificial

<220>
<223>  Human codon-optimized AGA open reading frame

<400>  1
atggcacgca agtcaaacct ccccgtcctg ctggtgcctt tcctgctgtg ccaagcactg       60

gtccggtgct cctcaccgct gccccttgtg gtgaacacct ggcccttcaa gaatgctacc      120

gaggcagcat ggagggctct tgcatcaggc ggttccgccc tggatgccgt ggagagcgga      180

tgtgctatgt gtgaacgcga acaatgcgac ggatcagtgg gattcggcgg atcacctgac      240

gagctcgggg aaactactct tgacgccatg attatggacg gaactaccat ggacgtgggt      300

gccgtgggcg atctccgccg cattaagaac gccattggag tggcccggaa ggtgcttgaa      360

cacaccaccc ataccctcct cgtcggagag tctgctacta ccttcgctca gtctatgggg      420

tttatcaatg aggatctgag caccaccgca tctcaggctc tccactcaga ctggctggcc      480

cgcaactgcc aaccgaacta ctggaggaat gtcatccctg atccatccaa gtattgcgga      540

ccttacaagc caccagggat tctgaaacag gacatcccca tccataaaga aaccgaggac      600

gaccggggac atgacactat cggaatggtg gtgatccata agaccgggca catcgccgct      660

ggaaccagca ccaatgggat caaattcaaa attcatggta gagtcggaga tagcccgatt      720

cctggtgctg gggcctacgc tgacgacact gccggagctg ccgcagctac cgggaatgga      780

gacatcctta tgaggttcct gccctcatac caagccgtgg aatacatgcg gagaggcgag      840

gacccaacca ttgcctgtca gaaggtgatc agccggattc agaagcactt ccccgagttc      900

ttcggtgccg tcatctgtgc aaacgtgact ggttcctacg gtgccgcctg caataagctt      960

agcaccttca ctcagttttc ttttatggtg tataactctg aaaagaacca gccaaccgag     1020

gagaaggtcg attgcatcta atga                                            1044


<210>  2
<211>  306
<212>  DNA
<213>  Artificial

<220>
<223>  Chicken beta-actin promoter

<400>  2
tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc       60

cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt      120

gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg gcggggcgag      180

gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg cgcgctccga      240

aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg      300

cgggcg                                                                 306


<210>  3
<211>  137
<212>  DNA
<213>  Artificial

<220>
<223>  Chicken beta-actin exon 1 and intron 1

<400>  3
ggagtcgctg cgacgctgcc ttcgccccgt gccccgctcc gccgccgcct cgcgccgccc       60

gccccggctc tgactgaccg cgttactccc acaggtgagc gggcgggacg gcccttctcc      120

tccgggctgt aattagc                                                     137


<210>  4
<211>  256
<212>  DNA
<213>  Artificial

<220>
<223>  Cytomegalovirus enhancer

<400>  4
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac       60

gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      120

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      180

caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc      240

tacttggcag tacatc                                                      256


<210>  5
<211>  92
<212>  DNA
<213>  Artificial

<220>
<223>  Hybrid/modified MVM intron

<400>  5
aagaggtaag ggtttaaggg atggttggtt ggtggggtat taatgtttaa ttacctggag       60

cacctgcctg aaatcacttt ttttcaggtt gg                                     92


<210>  6
<211>  254
<212>  DNA
<213>  Artificial

<220>
<223>  Bovine growth hormone polyadenylation signal

<400>  6
ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt       60

gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca      120

ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga      180

ggattgggaa gacaacagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc      240

ggaaagaacc agct                                                        254


<210>  7
<211>  2123
<212>  DNA
<213>  Artificial

<220>
<223>  AGA expression cassette

<400>  7
tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc gcccattgac       60

gtcaatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta      120

aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt      180

caatgacggt aaatggcccg cctggcattg tgcccagtac atgaccttat gggactttcc      240

tacttggcag tacatctacg tattagtcat cgctattacc atggtcgagg tgagccccac      300

gttctgcttc actctcccca tctccccccc ctccccaccc ccaattttgt atttatttat      360

tttttaatta ttttgtgcag cgatgggggc gggggggggg ggggggcgcg cgccaggcgg      420

ggcggggcgg ggcgaggggc ggggcggggc gaggcggaga ggtgcggcgg cagccaatca      480

gagcggcgcg ctccgaaagt ttccttttat ggcgaggcgg cggcggcggc ggccctataa      540

aaagcgaagc gcgcggcggg cgggagtcgc tgcgacgctg ccttcgcccc gtgccccgct      600

ccgccgccgc ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga      660

gcgggcggga cggcccttct cctccgggct gtaattagct gagcaagagg taagggttta      720

agggatggtt ggttggtggg gtattaatgt ttaattacct ggagcacctg cctgaaatca      780

ctttttttca ggttggaccg gtcgccacca tggcacgcaa gtcaaacctc cccgtcctgc      840

tggtgccttt cctgctgtgc caagcactgg tccggtgctc ctcaccgctg ccccttgtgg      900

tgaacacctg gcccttcaag aatgctaccg aggcagcatg gagggctctt gcatcaggcg      960

gttccgccct ggatgccgtg gagagcggat gtgctatgtg tgaacgcgaa caatgcgacg     1020

gatcagtggg attcggcgga tcacctgacg agctcgggga aactactctt gacgccatga     1080

ttatggacgg aactaccatg gacgtgggtg ccgtgggcga tctccgccgc attaagaacg     1140

ccattggagt ggcccggaag gtgcttgaac acaccaccca taccctcctc gtcggagagt     1200

ctgctactac cttcgctcag tctatggggt ttatcaatga ggatctgagc accaccgcat     1260

ctcaggctct ccactcagac tggctggccc gcaactgcca accgaactac tggaggaatg     1320

tcatccctga tccatccaag tattgcggac cttacaagcc accagggatt ctgaaacagg     1380

acatccccat ccataaagaa accgaggacg accggggaca tgacactatc ggaatggtgg     1440

tgatccataa gaccgggcac atcgccgctg gaaccagcac caatgggatc aaattcaaaa     1500

ttcatggtag agtcggagat agcccgattc ctggtgctgg ggcctacgct gacgacactg     1560

ccggagctgc cgcagctacc gggaatggag acatccttat gaggttcctg ccctcatacc     1620

aagccgtgga atacatgcgg agaggcgagg acccaaccat tgcctgtcag aaggtgatca     1680

gccggattca gaagcacttc cccgagttct tcggtgccgt catctgtgca aacgtgactg     1740

gttcctacgg tgccgcctgc aataagctta gcaccttcac tcagttttct tttatggtgt     1800

ataactctga aaagaaccag ccaaccgagg agaaggtcga ttgcatctaa tgagcggccg     1860

cggggatccc tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt     1920

gccttccttg accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat     1980

tgcatcgcat tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag     2040

caagggggag gattgggaag acaacagcag gcatgctggg gatgcggtgg gctctatggc     2100

ttctgaggcg gaaagaacca gct                                             2123


