                         SEQUENCE LISTING

<110>  SYNTHETIC GENOMICS, INC.
       WEINSTOCK, Matthew T
       GIBSON , Daniel G.
       STRIMLING, Daniel
 
<120>  VIBRIO SP. ORGANISMS WITH MODIFIED LIPOPOLYSACCHARIDE

<130>  SGI2110-1WO

<150>  US 62/588,755
<151>  2017-11-20

<160>  4     

<170>  PatentIn version 3.5

<210>  1
<211>  975
<212>  DNA
<213>  Vibrio natriegens


<220>
<221>  misc_feature
<223>  lpxL

<400>  1
atgggcacat ctggcgattc tttcatgaac aaacaaaaag agcctgtttt cgaggctcgg       60

ttcctaatgc ctcggtactg gggaactcta ctcatcatcg gggtgatgta tttacttagc      120

cttctacctt tcaaatttca attattcctg ggacgcagta ttggccgttt ggccatgcga      180

ttaatgaaaa agcgtcaggt caccattcgt cgcaatttag agctctgctt cccgaagatg      240

gacgagagca aacgagaagc gatactgaaa gctaacatcg ataattctgg cattgcactg      300

tttgagacag cgatggcttg gttctggccg gatcgcaggg ttaataaaca cgtaacgata      360

aaagggatgg aacacctaga agcgttagaa aaagacggca aaggggtgtt gatgcttgcc      420

gttcattcca tgaacttaga gctgggtgca cgtgcatttg gcattcaaaa atctggaatg      480

ggtgtttatc gaccgaataa caatccttgt tttgactatt ttcagtacaa gggtcgttcg      540

cgttccaatc gtactttgat cgatcgtaaa aatgttagag gaatgctgga cgctttgaat      600

tccggcaatc gcgtttggta tgcgcctgac catgattatg gaaccagaag atcgaccttt      660

gcgcctttgt ttgctgtcaa aaacgcctgt accacaacag gcaccagttt acttgttgat      720

gcaacagatt gtgcgattgt gccgtttacc atggtaagag gggatgatgg ccattacaca      780

ttaacgatca gggagccagt tgacggattt cctaaaggtg ataccagaaa cgcggcgatt      840

tttatcaata aaattgttga agagtcgatt atggctagcc ctagtcaata catgtggttg      900

caccgccgat ttaaaaccag accgcaaggt gaagattgtt tgtacaatcc tcagttaatc      960

cctgcgatga gttag                                                       975


<210>  2
<211>  981
<212>  DNA
<213>  Vibrio natriegens


<220>
<221>  misc_feature
<223>  lpxM

<400>  2
atgatcaata aacgtgatga tttcgatccc aaagcctata accctgagtt tgaccgctca       60

tttctcaagc ctaaatattg gggaacctgg ctcgctgttt ttagctcaat ttttatcgca      120

ttgctgcccc tcgcttttca ccagtggctg gcaaaagtgc tggcatcacg tttagttaaa      180

tctaaagcaa actcagtcaa caacgtaaag accaacttag ccttgtgttt cccacagtta      240

agtgaacaag agcgtgacca actggtttac aaaacgcttt atactgccgg cgtatttact      300

cttagatttg gcttggtctc tttgcgttca cccgaatggc tgcaatcaaa gtgtgacttt      360

gtaaactccg aacagttgct cgacctaact gagcaaaatc aaaaagtgat tttattagtg      420

ccgcactctt ggtcgattga tatacctgcg gtactacttg cctcacaagg cttacctgtt      480

tctgcgatgg cgaaaagaca gaaaaaccca gtaacagact ggctaatgca tcgccaacgc      540

gtgcaatatg gtggccgtgt gtacgaacgc tctggaggga ttaaaccctt catcaaatcg      600

attaaagacg gttacttggg ctactatcta ccagaccagg atcatggtgc tgaactcagt      660

gaatttgtgg actttttcgc gacgacgaag gcaaccctgc caggtctgac aaaactggcg      720

aaactgtcaa agtccaaagt aatcccgact tttgcctcac tggatccgga aactggtcgc      780

tttagtattg agttcatgcc tccactgacg cttcaggaaa ccgactgcga cgatgctcgc      840

tcactcaatg aagccattga gtactttgtg acgaaaaacc cagagcaata catgtggacc      900

ctgcgtctac tgcgtacaca ggcggatggc agtaacccat acagtgaaat gcgtgagcat      960

ggctttatta aagagaagtg a                                                981


<210>  3
<211>  5395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  Allelic exchange plasmid to delete lpxL

<400>  3
caaccatcat cgatgaattg cttcgttaat acagatgtag gtgttccaca gggtagccag       60

cagcatcctg cgatgcagat ccggatgcca tttcattacc tctttctccg cacccgacat      120

agatccgaag atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg      180

tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg      240

ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc      300

tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa      360

gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt      420

ggacaacaag ccagggatct gccatttcat tacctctttc tccgcacccg acatagatcc      480

ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac      540

cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg      600

ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc      660

gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg      720

agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt      780

tggtttgcgc attcacaggt cgtggaaacg atagggacgg atctgctggc gaaaggggga      840

tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa      900

acgacggcca gtgaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat      960

aggttaatgt catgataata atggtttctt agagcttacg gccagcctcg cagagcagga     1020

ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg     1080

ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct     1140

acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa     1200

atcgctataa tgaccccgaa gcagggttat gcagcggaaa agatccgtcg atcgacccag     1260

gtggcacttt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg     1320

aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg     1380

tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc     1440

ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg     1500

cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata     1560

tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta     1620

tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca     1680

agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt     1740

tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta     1800

ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa     1860

acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct     1920

ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc     1980

accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg     2040

ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca     2100

ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc     2160

ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc     2220

ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa     2280

caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga     2340

aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc     2400

ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac     2460

ccgttttttt gggaattcga gctctaagga ggttataaaa aatgcagttt aaggtttaca     2520

cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca     2580

cgccggggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct     2640

cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg     2700

atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg     2760

aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataattg atattgcctt     2820

gctgcaactt ggttacgata agcagaaaga tatccaccat caccaatatg cttatggcat     2880

gttcagtagt gaatcgagtt atcgacagct gggtttaatc gatgagcttg gagactacag     2940

acactttcag cgtgaggttg ggaagatgct actcaatact cccgagccta ttgatatgca     3000

tatctatttt gttatttctc aggagcatca caaagcggct aatacgctac attaatcctt     3060

tcactgtgcc gcaactgttt tcaatagcgt ggcacagttt tctagcttgt ttttccaaca     3120

catttttcta acacctttct ttatcgtcct ttccaaagcg tatgcgctgc attttcgggt     3180

aggaaaattc tatttattca aaagttcatg aaagtatgta ggtaatttac tctaaccaat     3240

gttaacgtgc tcaaatcttt tgtcgccttt acaatttaat tcgaagtcag tttgggttcc     3300

ctgtgtagtg tgtcgaagat ccataaaata cccgcgcttt aacttttttt gaagtgcaaa     3360

caatgataaa cgtttatgca attatcatta actttaatta cttatagtac cgttcgtata     3420

gcatacatta tacgaagtta ttgatgtccg gcggtgcttt tgccgttacg caccaccccg     3480

tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa     3540

caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata     3600

aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac     3660

cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc     3720

caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt     3780

ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat     3840

atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta     3900

taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca     3960

caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt     4020

ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac     4080

cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt     4140

ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta     4200

tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt     4260

caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat     4320

gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca     4380

tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga     4440

tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg     4500

tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg     4560

acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta     4620

ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt     4680

ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc     4740

agagcctgat aaaaacggtt aataacttcg tatagcatac attatacgaa cggtatgaat     4800

cgtttttagc tgttaatgct aagtagtggt ttaacatttt gaagccccca atcgttcacg     4860

attattgggg gctttttatt tggataagtg aaacgatacg gggattgagc ctcttttctc     4920

tcgataggct agatcactga ggcctgtcat ttctaacctg tttaatctgc tatttttttt     4980

acattcattg ggttgtatag tgaatgtact aagcttttct aacaatccgt ttttatcaag     5040

ccaacaccaa gaatcgagaa ctatgcccta tgtttgacga gttagctttt gccgggcttc     5100

tgtttagtcc gttggtggtt tttatgcctc ttgctttttt actgtcatgg agtacgcgtt     5160

ttgtgctgca taaatcaggc ttatacgcaa aactgtggaa ggcaccttgg tttgaagtca     5220

gcttgtatgt ctgttatctc gcgttagtca tttatctatt tgggagctaa tcatctatgg     5280

gaaaattcgt tcgcatctcc ctcactctta cgactttggt tattgccgtt attcttggcc     5340

attggatttg gcaacattat ctgtattcgc cttggacccg tgatggacga ataag          5395


<210>  4
<211>  5395
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetic


<220>
<221>  misc_feature
<223>  Allelic exchange plasmid to delete lpxM

<400>  4
caaccatcat cgatgaattg cttcgttaat acagatgtag gtgttccaca gggtagccag       60

cagcatcctg cgatgcagat ccggatgcca tttcattacc tctttctccg cacccgacat      120

agatccgaag atcagcagtt caacctgttg atagtacgta ctaagctctc atgtttcacg      180

tactaagctc tcatgtttaa cgtactaagc tctcatgttt aacgaactaa accctcatgg      240

ctaacgtact aagctctcat ggctaacgta ctaagctctc atgtttcacg tactaagctc      300

tcatgtttga acaataaaat taatataaat cagcaactta aatagcctct aaggttttaa      360

gttttataag aaaaaaaaga atatataagg cttttaaagc ttttaaggtt taacggttgt      420

ggacaacaag ccagggatct gccatttcat tacctctttc tccgcacccg acatagatcc      480

ggaacataat ggtgcagggc gctgacttcc gcgtttccag actttacgaa acacggaaac      540

cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt gcagcagcag tcgcttcacg      600

ttcgctcgcg tatcggtgat tcattctgct aaccagtaag gcaaccccgc cagcctagcc      660

gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg ccaggaccca acgctgcccg      720

agatgcgccg cgtgcggctg ctggagatgg cggacgcgat ggatatgttc tgccaagggt      780

tggtttgcgc attcacaggt cgtggaaacg atagggacgg atctgctggc gaaaggggga      840

tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa      900

acgacggcca gtgaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat      960

aggttaatgt catgataata atggtttctt agagcttacg gccagcctcg cagagcagga     1020

ttcccgttga gcaccgccag gtgcgaataa gggacagtga agaaggaaca cccgctcgcg     1080

ggtgggccta cttcacctat cctgcccggc tgacgccgtt ggatacacca aggaaagtct     1140

acacgaaccc tttggcaaaa tcctgtatat cgtgcgaaaa aggatggata taccgaaaaa     1200

atcgctataa tgaccccgaa gcagggttat gcagcggaaa agatccgtcg atcgacccag     1260

gtggcacttt atgacaactt gacggctaca tcattcactt tttcttcaca accggcacgg     1320

aactcgctcg ggctggcccc ggtgcatttt ttaaataccc gcgagaaata gagttgatcg     1380

tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc gggtggtgct caaaagcagc     1440

ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga cgctaatccc taactgctgg     1500

cggaaaagat gtgacagacg cgacggcgac aagcaaacat gctgtgcgac gctggcgata     1560

tcaaaattgc tgtctgccag gtgatcgctg atgtactgac aagcctcgcg tacccgatta     1620

tccatcggtg gatggagcga ctcgttaatc gcttccatgc gccgcagtaa caattgctca     1680

agcagattta tcgccagcag ctccgaatag cgcccttccc cttgcccggc gttaatgatt     1740

tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat ccgggcgaaa gaaccccgta     1800

ttggcaaata ttgacggcca gttaagccat tcatgccagt aggcgcgcgg acgaaagtaa     1860

acccactggt gataccattc gcgagcctcc ggatgacgac cgtagtgatg aatctctcct     1920

ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt ctcgtccctg atttttcacc     1980

accccctgac cgcgaatggt gagattgaga atataacctt tcattcccag cggtcggtcg     2040

ataaaaaaat cgagataacc gttggcctca atcggcgtta aacccgccac cagatgggca     2100

ttaaacgagt atcccggcag caggggatca ttttgcgctt cagccatact tttcatactc     2160

ccgccattca gagaagaaac caattgtcca tattgcatca gacattgccg tcactgcgtc     2220

ttttactggc tcttctcgct aaccaaaccg gtaaccccgc ttattaaaag cattctgtaa     2280

caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa gtgtctataa tcacggcaga     2340

aaagtccaca ttgattattt gcacggcgtc acactttgct atgccatagc atttttatcc     2400

ataagattag cggatcctac ctgacgcttt ttatcgcaac tctctactgt ttctccatac     2460

ccgttttttt gggaattcga gctctaagga ggttataaaa aatgcagttt aaggtttaca     2520

cctataaaag agagagccgt tatcgtctgt ttgtggatgt acagagtgat attattgaca     2580

cgccggggcg acggatggtg atccccctgg ccagtgcacg tctgctgtca gataaagtct     2640

cccgtgaact ttacccggtg gtgcatatcg gggatgaaag ctggcgcatg atgaccaccg     2700

atatggccag tgtgccggtc tccgttatcg gggaagaagt ggctgatctc agccaccgcg     2760

aaaatgacat caaaaacgcc attaacctga tgttctgggg aatataaagc acggtgagac     2820

cctatctcaa atcacgggct tccgttactt caatgtttac ggccctcgtg aagaccataa     2880

aggaagcatg gcatccgttg ccttccacct aaataaccag ctaaatgcag gtgaaaaccc     2940

gaaactcttc gcaggtagcg agacctttaa acgtgatttc gtttacgtcg gcgatgtatg     3000

caaagtaaat ctgtggttct tcgaaaatgg cgtatcaggc atttttaact gtggcacagg     3060

acgcgcggag tcgtttgaag aggtcgcgaa agcggtgatc actcaccacg gaaaaggtga     3120

aatcgaaacg attcctttcc ctgagcatct aaaaggcgct taccaagagt tcactcaagc     3180

tgatctgact aagctacgct cagcaggctg cgacgtagaa ttcaaaacgg ttgcagaagg     3240

tgtctctgaa tacctagcca tccaaaatcg ctaagtatat ttgttcgcaa taatgaggtg     3300

gcggtgatta tgacctgcca cctttactca gcttcaatag cctgtatcat ttcctttatc     3360

tgcatttaaa tcactcgtct ccatacgcat aggtaactag actcccctac cgttcgtata     3420

gcatacatta tacgaagtta ttgatgtccg gcggtgcttt tgccgttacg caccaccccg     3480

tcagtagctg aacaggaggg acagctgata gaaacagaag ccactggagc acctcaaaaa     3540

caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata     3600

aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac     3660

cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc     3720

caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt     3780

ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat     3840

atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta     3900

taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca     3960

caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt     4020

ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac     4080

cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt     4140

ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta     4200

tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt     4260

caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat     4320

gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca     4380

tgccgtctgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga     4440

tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg     4500

tgctacgcct gaataagtga taataagcgg atgaatggca gaaattcgaa agcaaattcg     4560

acccggtcgt cggttcaggg cagggtcgtt aaatagccgc ttatgtctat tgctggttta     4620

ccggtttatt gactaccgga agcagtgtga ccgtgtgctt ctcaaatgcc tgaggccagt     4680

ttgctcaggc tctccccgtg gaggtaataa ttgacgatat gatcatttat tctgcctccc     4740

agagcctgat aaaaacggtt aataacttcg tatagcatac attatacgaa cggtatatca     4800

acatgtcgaa aaacaaaatt ctaattattg gtccttcttg ggttggagat atggtgatgt     4860

cgcaatccct atacattgtt ttaaagcaac tgaaccctga aagcgaaatt gatgtgattg     4920

cacccgggtg gtgtaaacca atcttagagc gtatgcctga ggtgaataag gccattgaaa     4980

tgccgattgg gcatggtgaa tttaaccttc tggggcggcg tgaaattggt aaaacgctgc     5040

gtgagaagaa atacgatcac gcttatatcc tgccaaaatc ggcgaaatcc gctctgatac     5100

cttggtttgc taacattcct cttcgcaccg gctggaaagg cgaaatgcgc tatggcttgc     5160

tgaatgacat tcgtcctaac atgaagtcgt tccaatacat ggtggagcgt tatgtggctt     5220

tggcttatcc acaatcggag atggtggact catcgtcact aggtggatta gagacgctgc     5280

cgcgccctcg cctagctatc aatactgaag agcaacaaac cactatccag aagtttaacc     5340

tcaataaaga acgctctatt attggcttgt gcccaggtgc agaatttggt ccggc          5395


