                         SEQUENCE LISTING

<110>  Howard Hughes Medical Institute
 
<120>  GENETICALLY ENCODED CALCIUM INDICATORS AND METHODS OF USE

<130>  30872-0019WO1

<150>  US 62/589,739
<151>  2017-11-22

<160>  8     

<170>  PatentIn version 3.5

<210>  1
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetically generated nucleic acid encoding peptide biosensor

<400>  1
atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg       60

ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt      120

cgtaagtgga ataagacagg tcacgcagtc agagtgatag gtcggctgag ctcactcgag      180

aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt ccacatccgc      240

cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc      300

ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg      360

aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg      420

atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag      480

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac      540

ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc      600

ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc      660

ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc      720

ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac      780

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc      840

gagcttaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac      900

aaccttcctg accaactgac tgaagagcag atcgcagaat ttaaagagct tttctcccta      960

tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct     1020

ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac     1080

ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac     1140

agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc     1200

tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat     1260

gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac     1320

gaagagtttg tacaaatgat gacagcgaag                                      1350


<210>  2
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide biosensor

<400>  2

Met Gly Ser His His His His His His Gly Met Ala Ser Met Thr Gly 
1               5                   10                  15      


Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp Leu 
            20                  25                  30          


Ala Thr Met Val Asp Ser Ser Arg Arg Lys Trp Asn Lys Thr Gly His 
        35                  40                  45              


Ala Val Arg Val Ile Gly Arg Leu Ser Ser Leu Glu Asn Val Tyr Ile 
    50                  55                  60                  


Lys Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe His Ile Arg 
65                  70                  75                  80  


His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala Tyr His Tyr Gln Gln 
                85                  90                  95      


Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr 
            100                 105                 110         


Leu Ser Val Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp 
        115                 120                 125             


His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly 
    130                 135                 140                 


Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys 
145                 150                 155                 160 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                165                 170                 175     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            180                 185                 190         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        195                 200                 205             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    210                 215                 220                 


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
225                 230                 235                 240 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg Thr Ile Phe 
                245                 250                 255     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            260                 265                 270         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        275                 280                 285             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Leu Pro Asp 
    290                 295                 300                 


Gln Leu Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Leu Phe Ser Leu 
305                 310                 315                 320 


Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr 
                325                 330                 335     


Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp 
            340                 345                 350         


Met Ile Asn Glu Val Asp Ala Asp Gly Asp Gly Thr Ile Asp Phe Pro 
        355                 360                 365             


Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys Tyr Arg Asp Thr Glu 
    370                 375                 380                 


Glu Glu Ile Arg Glu Ala Phe Gly Val Phe Asp Lys Asp Gly Asn Gly 
385                 390                 395                 400 


Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu 
                405                 410                 415     


Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile 
            420                 425                 430         


Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr 
        435                 440                 445             


Ala Lys 
    450 


<210>  3
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetically generated nucleic acid encoding peptide biosensor

<400>  3
atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg       60

ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt      120

cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag      180

aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc      240

cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc      300

ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg      360

aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg      420

atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag      480

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac      540

ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc      600

ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc      660

ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc      720

ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac      780

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc      840

gagcttaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac      900

aacctgccgg accaactgac tgaagagcag atcgcagaat ttaaagagct gttctcccta      960

tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct     1020

ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac     1080

ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa aatgaaatac     1140

acagacagtg aagaagaaat tagagaagcg ttccgtgtgt ttgataagga tggcaatggc     1200

tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat     1260

gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac     1320

gaagagtttg tacaaatgat gacagcgaag                                      1350


<210>  4
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide biosensor

<400>  4

Met Gly Ser His His His His His His Gly Met Ala Ser Met Thr Gly 
1               5                   10                  15      


Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp Leu 
            20                  25                  30          


Ala Thr Met Val Asp Ser Ser Arg Arg Lys Trp Asn Lys Thr Gly His 
        35                  40                  45              


Ala Val Arg Ala Ile Gly Arg Leu Ser Ser Leu Glu Asn Val Tyr Ile 
    50                  55                  60                  


Lys Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg 
65                  70                  75                  80  


His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala Tyr His Tyr Gln Gln 
                85                  90                  95      


Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr 
            100                 105                 110         


Leu Ser Val Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp 
        115                 120                 125             


His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly 
    130                 135                 140                 


Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys 
145                 150                 155                 160 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                165                 170                 175     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            180                 185                 190         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        195                 200                 205             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    210                 215                 220                 


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
225                 230                 235                 240 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg Thr Ile Phe 
                245                 250                 255     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            260                 265                 270         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        275                 280                 285             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Leu Pro Asp 
    290                 295                 300                 


Gln Leu Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Leu Phe Ser Leu 
305                 310                 315                 320 


Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr 
                325                 330                 335     


Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp 
            340                 345                 350         


Met Ile Asn Glu Val Asp Ala Asp Gly Asp Gly Thr Ile Asp Phe Pro 
        355                 360                 365             


Glu Phe Leu Thr Met Met Ala Arg Lys Met Lys Tyr Thr Asp Ser Glu 
    370                 375                 380                 


Glu Glu Ile Arg Glu Ala Phe Arg Val Phe Asp Lys Asp Gly Asn Gly 
385                 390                 395                 400 


Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu 
                405                 410                 415     


Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile 
            420                 425                 430         


Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr 
        435                 440                 445             


Ala Lys 
    450 


<210>  5
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetically generated nucleic acid encoding peptide biosensor

<400>  5
atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg       60

ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt      120

cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag      180

aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc      240

cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc      300

ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg      360

aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg      420

atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag      480

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac      540

ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc      600

ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc      660

ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc      720

ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac      780

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc      840

gagcttaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac      900

aacccacctg accaactgac tgaagagcag atcgcagaat ttaaagagct tttctcccta      960

tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct     1020

ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac     1080

ggtgacggca caatcgactt ccctgagttc ctgacaatgt acgcaagaaa aatgaaatac     1140

agggacacgg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc     1200

tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat     1260

gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac     1320

gaagagtttg tacaaatgat gacagcgaag                                      1350


<210>  6
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide biosensor

<400>  6

Met Gly Ser His His His His His His Gly Met Ala Ser Met Thr Gly 
1               5                   10                  15      


Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp Leu 
            20                  25                  30          


Ala Thr Met Val Asp Ser Ser Arg Arg Lys Trp Asn Lys Thr Gly His 
        35                  40                  45              


Ala Val Arg Ala Ile Gly Arg Leu Ser Ser Leu Glu Asn Val Tyr Ile 
    50                  55                  60                  


Lys Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg 
65                  70                  75                  80  


His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala Tyr His Tyr Gln Gln 
                85                  90                  95      


Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr 
            100                 105                 110         


Leu Ser Val Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp 
        115                 120                 125             


His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly 
    130                 135                 140                 


Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys 
145                 150                 155                 160 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                165                 170                 175     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            180                 185                 190         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        195                 200                 205             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    210                 215                 220                 


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
225                 230                 235                 240 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg Thr Ile Phe 
                245                 250                 255     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            260                 265                 270         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        275                 280                 285             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Pro Pro Asp 
    290                 295                 300                 


Gln Leu Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Leu Phe Ser Leu 
305                 310                 315                 320 


Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr 
                325                 330                 335     


Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp 
            340                 345                 350         


Met Ile Asn Glu Val Asp Ala Asp Gly Asp Gly Thr Ile Asp Phe Pro 
        355                 360                 365             


Glu Phe Leu Thr Met Tyr Ala Arg Lys Met Lys Tyr Arg Asp Thr Glu 
    370                 375                 380                 


Glu Glu Ile Arg Glu Ala Phe Gly Val Phe Asp Lys Asp Gly Asn Gly 
385                 390                 395                 400 


Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu 
                405                 410                 415     


Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile 
            420                 425                 430         


Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr 
        435                 440                 445             


Ala Lys 
    450 


<210>  7
<211>  1350
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Synthetically generated nucleic acid encoding peptide biosensor

<400>  7
atgggttctc atcatcatca tcatcatggt atggctagca tgactggtgg acagcaaatg       60

ggtcgggatc tgtacgacga tgacgataag gatctcgcca ccatggtcga ctcatcacgt      120

cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcacagcct      180

aacgtctata tcaaggccga caagcagaag aacggcatca aggcgaactt caagatccgc      240

cacaacatcg aggacggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc      300

ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc caaactttcg      360

aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg      420

atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcat ggtgagcaag      480

ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac      540

ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg caagctgacc      600

ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc      660

ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc      720

ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt caaggacgac      780

ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc      840

gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac      900

aaccttcctg accaactgac tgaagagcag atcgcagaat ttaaagagct tttctcccta      960

tttgacaagg acggggatgg gacaataaca accaaggagc tggggacggt gatgcggtct     1020

ctggggcaga accccacaga agcagagctg caggacatga tcaatgaagt agatgccgac     1080

ggtgacggca caatcgactt ccctgagttc ctgacaatga tggcaagaaa agggagctac     1140

agggacagtg aagaagaaat tagagaagcg ttcggtgtgt ttgataagga tggcaatggc     1200

tacatcagtg cagcagagct tcgccacgtg atgacaaacc ttggagagaa gttaacagat     1260

gaagaggttg atgaaatgat cagggaagca gacatcgatg gggatggtca ggtaaactac     1320

gaagagtttg tacaaatgat gacagcgaag                                      1350


<210>  8
<211>  450
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic peptide biosensor

<400>  8

Met Gly Ser His His His His His His Gly Met Ala Ser Met Thr Gly 
1               5                   10                  15      


Gly Gln Gln Met Gly Arg Asp Leu Tyr Asp Asp Asp Asp Lys Asp Leu 
            20                  25                  30          


Ala Thr Met Val Asp Ser Ser Arg Arg Lys Trp Asn Lys Thr Gly His 
        35                  40                  45              


Ala Val Arg Ala Ile Gly Arg Leu Ser Ser Gln Pro Asn Val Tyr Ile 
    50                  55                  60                  


Lys Ala Asp Lys Gln Lys Asn Gly Ile Lys Ala Asn Phe Lys Ile Arg 
65                  70                  75                  80  


His Asn Ile Glu Asp Gly Gly Val Gln Leu Ala Tyr His Tyr Gln Gln 
                85                  90                  95      


Asn Thr Pro Ile Gly Asp Gly Pro Val Leu Leu Pro Asp Asn His Tyr 
            100                 105                 110         


Leu Ser Val Gln Ser Lys Leu Ser Lys Asp Pro Asn Glu Lys Arg Asp 
        115                 120                 125             


His Met Val Leu Leu Glu Phe Val Thr Ala Ala Gly Ile Thr Leu Gly 
    130                 135                 140                 


Met Asp Glu Leu Tyr Lys Gly Gly Thr Gly Gly Ser Met Val Ser Lys 
145                 150                 155                 160 


Gly Glu Glu Leu Phe Thr Gly Val Val Pro Ile Leu Val Glu Leu Asp 
                165                 170                 175     


Gly Asp Val Asn Gly His Lys Phe Ser Val Ser Gly Glu Gly Glu Gly 
            180                 185                 190         


Asp Ala Thr Tyr Gly Lys Leu Thr Leu Lys Phe Ile Cys Thr Thr Gly 
        195                 200                 205             


Lys Leu Pro Val Pro Trp Pro Thr Leu Val Thr Thr Leu Thr Tyr Gly 
    210                 215                 220                 


Val Gln Cys Phe Ser Arg Tyr Pro Asp His Met Lys Gln His Asp Phe 
225                 230                 235                 240 


Phe Lys Ser Ala Met Pro Glu Gly Tyr Ile Gln Glu Arg Thr Ile Phe 
                245                 250                 255     


Phe Lys Asp Asp Gly Asn Tyr Lys Thr Arg Ala Glu Val Lys Phe Glu 
            260                 265                 270         


Gly Asp Thr Leu Val Asn Arg Ile Glu Leu Lys Gly Ile Asp Phe Lys 
        275                 280                 285             


Glu Asp Gly Asn Ile Leu Gly His Lys Leu Glu Tyr Asn Leu Pro Asp 
    290                 295                 300                 


Gln Leu Thr Glu Glu Gln Ile Ala Glu Phe Lys Glu Leu Phe Ser Leu 
305                 310                 315                 320 


Phe Asp Lys Asp Gly Asp Gly Thr Ile Thr Thr Lys Glu Leu Gly Thr 
                325                 330                 335     


Val Met Arg Ser Leu Gly Gln Asn Pro Thr Glu Ala Glu Leu Gln Asp 
            340                 345                 350         


Met Ile Asn Glu Val Asp Ala Asp Gly Asp Gly Thr Ile Asp Phe Pro 
        355                 360                 365             


Glu Phe Leu Thr Met Met Ala Arg Lys Gly Ser Tyr Arg Asp Ser Glu 
    370                 375                 380                 


Glu Glu Ile Arg Glu Ala Phe Gly Val Phe Asp Lys Asp Gly Asn Gly 
385                 390                 395                 400 


Tyr Ile Ser Ala Ala Glu Leu Arg His Val Met Thr Asn Leu Gly Glu 
                405                 410                 415     


Lys Leu Thr Asp Glu Glu Val Asp Glu Met Ile Arg Glu Ala Asp Ile 
            420                 425                 430         


Asp Gly Asp Gly Gln Val Asn Tyr Glu Glu Phe Val Gln Met Met Thr 
        435                 440                 445             


Ala Lys 
    450 


