                         SEQUENCE LISTING

<110>  Jazz Pharmaceuticals Ireland Ltd.
       Pine, Polly
       Gursahani, Hemamalini
 
<120>  Methods of Treatment with Asparaginase

<130>  061834-5065

<160>  7     

<170>  PatentIn version 3.5

<210>  1
<211>  327
<212>  PRT
<213>  Erwinia chrysanthemi

<400>  1

Ala Asp Lys Leu Pro Asn Ile Val Ile Leu Ala Thr Gly Gly Thr Ile 
1               5                   10                  15      


Ala Gly Ser Ala Ala Thr Gly Thr Gln Thr Thr Gly Tyr Lys Ala Gly 
            20                  25                  30          


Ala Leu Gly Val Asp Thr Leu Ile Asn Ala Val Pro Glu Val Lys Lys 
        35                  40                  45              


Leu Ala Asn Val Lys Gly Glu Gln Phe Ser Asn Met Ala Ser Glu Asn 
    50                  55                  60                  


Met Thr Gly Asp Val Val Leu Lys Leu Ser Gln Arg Val Asn Glu Leu 
65                  70                  75                  80  


Leu Ala Arg Asp Asp Val Asp Gly Val Val Ile Thr His Gly Thr Asp 
                85                  90                  95      


Thr Val Glu Glu Ser Ala Tyr Phe Leu His Leu Thr Val Lys Ser Asp 
            100                 105                 110         


Lys Pro Val Val Phe Val Ala Ala Met Arg Pro Ala Thr Ala Ile Ser 
        115                 120                 125             


Ala Asp Gly Pro Met Asn Leu Leu Glu Ala Val Arg Val Ala Gly Asp 
    130                 135                 140                 


Lys Gln Ser Arg Gly Arg Gly Val Met Val Val Leu Asn Asp Arg Ile 
145                 150                 155                 160 


Gly Ser Ala Arg Tyr Ile Thr Lys Thr Asn Ala Ser Thr Leu Asp Thr 
                165                 170                 175     


Phe Lys Ala Asn Glu Glu Gly Tyr Leu Gly Val Ile Ile Gly Asn Arg 
            180                 185                 190         


Ile Tyr Tyr Gln Asn Arg Ile Asp Lys Leu His Thr Thr Arg Ser Val 
        195                 200                 205             


Phe Asp Val Arg Gly Leu Thr Ser Leu Pro Lys Val Asp Ile Leu Tyr 
    210                 215                 220                 


Gly Tyr Gln Asp Asp Pro Glu Tyr Leu Tyr Asp Ala Ala Ile Gln His 
225                 230                 235                 240 


Gly Val Lys Gly Ile Val Tyr Ala Gly Met Gly Ala Gly Ser Val Ser 
                245                 250                 255     


Val Arg Gly Ile Ala Gly Met Arg Lys Ala Met Glu Lys Gly Val Val 
            260                 265                 270         


Val Ile Arg Ser Thr Arg Thr Gly Asn Gly Ile Val Pro Pro Asp Glu 
        275                 280                 285             


Glu Leu Pro Gly Leu Val Ser Asp Ser Leu Asn Pro Ala His Ala Arg 
    290                 295                 300                 


Ile Leu Leu Met Leu Ala Leu Thr Arg Thr Ser Asp Pro Lys Val Ile 
305                 310                 315                 320 


Gln Glu Tyr Phe His Thr Tyr 
                325         


<210>  2
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sythetic PA(20) peptide

<400>  2

Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      


Ala Ala Pro Ala 
            20  


<210>  3
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PA(40) peptide

<400>  3

Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            20                  25                  30          


Ala Pro Ala Pro Ala Ala Pro Ala 
        35                  40  


<210>  4
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PA(20) peptide

<400>  4

Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      


Ala Ala Pro Ala 
            20  


<210>  5
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PA(40)-polypeptide

<400>  5

Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            20                  25                  30          


Ala Pro Ala Pro Ala Ala Pro Ala 
        35                  40  


<210>  6
<211>  528
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic  PA(200)-fusion protein

<400>  6

Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            20                  25                  30          


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro 
        35                  40                  45              


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala 
    50                  55                  60                  


Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
65                  70                  75                  80  


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
                85                  90                  95      


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            100                 105                 110         


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro 
        115                 120                 125             


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala 
    130                 135                 140                 


Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
145                 150                 155                 160 


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
                165                 170                 175     


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            180                 185                 190         


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Asp Lys Leu Pro Asn Ile 
        195                 200                 205             


Val Ile Leu Ala Thr Gly Gly Thr Ile Ala Gly Ser Ala Ala Thr Gly 
    210                 215                 220                 


Thr Gln Thr Thr Gly Tyr Lys Ala Gly Ala Leu Gly Val Asp Thr Leu 
225                 230                 235                 240 


Ile Asn Ala Val Pro Glu Val Lys Lys Leu Ala Asn Val Lys Gly Glu 
                245                 250                 255     


Gln Phe Ser Asn Met Ala Ser Glu Asn Met Thr Gly Asp Val Val Leu 
            260                 265                 270         


Lys Leu Ser Gln Arg Val Asn Glu Leu Leu Ala Arg Asp Asp Val Asp 
        275                 280                 285             


Gly Val Val Ile Thr His Gly Thr Asp Thr Val Glu Glu Ser Ala Tyr 
    290                 295                 300                 


Phe Leu His Leu Thr Val Lys Ser Asp Lys Pro Val Val Phe Val Ala 
305                 310                 315                 320 


Ala Met Arg Pro Ala Thr Ala Ile Ser Ala Asp Gly Pro Met Asn Leu 
                325                 330                 335     


Leu Glu Ala Val Arg Val Ala Gly Asp Lys Gln Ser Arg Gly Arg Gly 
            340                 345                 350         


Val Met Val Val Leu Asn Asp Arg Ile Gly Ser Ala Arg Tyr Ile Thr 
        355                 360                 365             


Lys Thr Asn Ala Ser Thr Leu Asp Thr Phe Lys Ala Asn Glu Glu Gly 
    370                 375                 380                 


Tyr Leu Gly Val Ile Ile Gly Asn Arg Ile Tyr Tyr Gln Asn Arg Ile 
385                 390                 395                 400 


Asp Lys Leu His Thr Thr Arg Ser Val Phe Asp Val Arg Gly Leu Thr 
                405                 410                 415     


Ser Leu Pro Lys Val Asp Ile Leu Tyr Gly Tyr Gln Asp Asp Pro Glu 
            420                 425                 430         


Tyr Leu Tyr Asp Ala Ala Ile Gln His Gly Val Lys Gly Ile Val Tyr 
        435                 440                 445             


Ala Gly Met Gly Ala Gly Ser Val Ser Val Arg Gly Ile Ala Gly Met 
    450                 455                 460                 


Arg Lys Ala Met Glu Lys Gly Val Val Val Ile Arg Ser Thr Arg Thr 
465                 470                 475                 480 


Gly Asn Gly Ile Val Pro Pro Asp Glu Glu Leu Pro Gly Leu Val Ser 
                485                 490                 495     


Asp Ser Leu Asn Pro Ala His Ala Arg Ile Leu Leu Met Leu Ala Leu 
            500                 505                 510         


Thr Arg Thr Ser Asp Pro Lys Val Ile Gln Glu Tyr Phe His Thr Tyr 
        515                 520                 525             


<210>  7
<211>  728
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic PA(400) peptide

<400>  7

Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
1               5                   10                  15      


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            20                  25                  30          


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro 
        35                  40                  45              


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala 
    50                  55                  60                  


Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
65                  70                  75                  80  


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
                85                  90                  95      


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            100                 105                 110         


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro 
        115                 120                 125             


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala 
    130                 135                 140                 


Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
145                 150                 155                 160 


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
                165                 170                 175     


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            180                 185                 190         


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro 
        195                 200                 205             


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala 
    210                 215                 220                 


Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
225                 230                 235                 240 


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
                245                 250                 255     


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            260                 265                 270         


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro 
        275                 280                 285             


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala 
    290                 295                 300                 


Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
305                 310                 315                 320 


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro 
                325                 330                 335     


Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
            340                 345                 350         


Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala Ala Pro Ala Pro 
        355                 360                 365             


Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala Ala Ala Pro Ala 
    370                 375                 380                 


Ala Pro Ala Pro Ala Ala Pro Ala Ala Pro Ala Pro Ala Ala Pro Ala 
385                 390                 395                 400 


Ala Ala Asp Lys Leu Pro Asn Ile Val Ile Leu Ala Thr Gly Gly Thr 
                405                 410                 415     


Ile Ala Gly Ser Ala Ala Thr Gly Thr Gln Thr Thr Gly Tyr Lys Ala 
            420                 425                 430         


Gly Ala Leu Gly Val Asp Thr Leu Ile Asn Ala Val Pro Glu Val Lys 
        435                 440                 445             


Lys Leu Ala Asn Val Lys Gly Glu Gln Phe Ser Asn Met Ala Ser Glu 
    450                 455                 460                 


Asn Met Thr Gly Asp Val Val Leu Lys Leu Ser Gln Arg Val Asn Glu 
465                 470                 475                 480 


Leu Leu Ala Arg Asp Asp Val Asp Gly Val Val Ile Thr His Gly Thr 
                485                 490                 495     


Asp Thr Val Glu Glu Ser Ala Tyr Phe Leu His Leu Thr Val Lys Ser 
            500                 505                 510         


Asp Lys Pro Val Val Phe Val Ala Ala Met Arg Pro Ala Thr Ala Ile 
        515                 520                 525             


Ser Ala Asp Gly Pro Met Asn Leu Leu Glu Ala Val Arg Val Ala Gly 
    530                 535                 540                 


Asp Lys Gln Ser Arg Gly Arg Gly Val Met Val Val Leu Asn Asp Arg 
545                 550                 555                 560 


Ile Gly Ser Ala Arg Tyr Ile Thr Lys Thr Asn Ala Ser Thr Leu Asp 
                565                 570                 575     


Thr Phe Lys Ala Asn Glu Glu Gly Tyr Leu Gly Val Ile Ile Gly Asn 
            580                 585                 590         


Arg Ile Tyr Tyr Gln Asn Arg Ile Asp Lys Leu His Thr Thr Arg Ser 
        595                 600                 605             


Val Phe Asp Val Arg Gly Leu Thr Ser Leu Pro Lys Val Asp Ile Leu 
    610                 615                 620                 


Tyr Gly Tyr Gln Asp Asp Pro Glu Tyr Leu Tyr Asp Ala Ala Ile Gln 
625                 630                 635                 640 


His Gly Val Lys Gly Ile Val Tyr Ala Gly Met Gly Ala Gly Ser Val 
                645                 650                 655     


Ser Val Arg Gly Ile Ala Gly Met Arg Lys Ala Met Glu Lys Gly Val 
            660                 665                 670         


Val Val Ile Arg Ser Thr Arg Thr Gly Asn Gly Ile Val Pro Pro Asp 
        675                 680                 685             


Glu Glu Leu Pro Gly Leu Val Ser Asp Ser Leu Asn Pro Ala His Ala 
    690                 695                 700                 


Arg Ile Leu Leu Met Leu Ala Leu Thr Arg Thr Ser Asp Pro Lys Val 
705                 710                 715                 720 


Ile Gln Glu Tyr Phe His Thr Tyr 
                725             


