﻿                         序列表

<110>  北京韩美药品有限公司

<120>  抗PD-L1/抗CD47天然抗体结构样异源二聚体形式双特异抗体及其制备

<130>  LZ1707727CN01

<160>  14

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  321
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人PD-L1抗体的轻链可变区核苷酸序列


<400>  1
gacatccaga tgacccagag ccctagcagc ctgagcgcta gcgtgggcga cagggtgacc        60
atcacctgca gggccagcca ggatgtgagc accgctgtgg cctggtatca acagaagccc       120
ggcaaggccc ccaagctgct gatctacagc gccagcttcc tgtacagcgg cgtgcccagc       180
agatttagcg gcagcggcag cggcaccgat ttcaccctga ccatcagcag cctgcagccc       240
gaggacttcg ccacctacta ctgccagcag tacctgtacc atcccgccac cttcggccag       300
ggcaccaagg tggagatcaa g                                                 321

<210>  2
<211>  107
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人PD-L1抗体的轻链可变区氨基酸序列


<400>  2
Asp Ile Gln Met Thr Gln Ser Pro Ser Ser Leu Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Arg Ala Ser Gln Asp Val Ser Thr Ala 
            20                  25                  30          
Val Ala Trp Tyr Gln Gln Lys Pro Gly Lys Ala Pro Lys Leu Leu Ile 
        35                  40                  45              
Tyr Ser Ala Ser Phe Leu Tyr Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Gln Gln Tyr Leu Tyr His Pro Ala 
                85                  90                  95      
Thr Phe Gly Gln Gly Thr Lys Val Glu Ile Lys 
            100                 105         

<210>  3
<211>  321
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人PD-L1抗体轻链恒定区核苷酸序列


<400>  3
cgaactgtgg ccgctccaag cgtcttcatt tttccaccct ctgacgaaca gctgaagtca        60
gggacagctt ccgtggtctg tctgctgaac aatttttacc ccagggaggc caaagtgcag       120
tggaaggtcg ataacgctct gcagagcgga aattctcagg agagtgtgac agaacaggac       180
tcaaaagatt ccacttatag cctgtctagt accctgacac tgtccaaggc agactacgaa       240
aagcataaag tgtatgcctg tgaggtcaca catcagggtc tgtcaagccc cgtcactaag       300
tccttcaatc gtggcgaatg c                                                 321

<210>  4
<211>  107
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人PD-L1抗体轻链恒定区氨基酸序列


<400>  4
Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      
Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          
Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              
Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  
Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  
Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      
Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         

<210>  5
<211>  354
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人PD-L1抗体重链可变区核苷酸序列


<400>  5
gaggtgcagc tggtggagag cggaggagga ctggtgcagc ctggaggatc cctgagactg        60
agctgcgccg ccagcggctt caccttcagc gacagctgga tccactgggt gagacaggcc       120
cctggcaagg gcctggaatg ggtggcctgg atctcccctt acggcggcag cacctactac       180
gccgacagcg tgaagggcag gttcaccatc agcgccgaca ccagcaagaa caccgcctac       240
ctgcagatga acagcctgag ggccgaggac accgccgtgt actactgcgc cagaagacac       300
tggcccggcg gattcgacta ctggggacag ggcaccctgg tgaccgtgag cgcc             354

<210>  6
<211>  118
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人PD-L1抗体重链可变区氨基酸序列


<400>  6
Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      
Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Ser Asp Ser 
            20                  25                  30          
Trp Ile His Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              
Ala Trp Ile Ser Pro Tyr Gly Gly Ser Thr Tyr Tyr Ala Asp Ser Val 
    50                  55                  60                  
Lys Gly Arg Phe Thr Ile Ser Ala Asp Thr Ser Lys Asn Thr Ala Tyr 
65                  70                  75                  80  
Leu Gln Met Asn Ser Leu Arg Ala Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Ala Arg Arg His Trp Pro Gly Gly Phe Asp Tyr Trp Gly Gln Gly Thr 
            100                 105                 110         
Leu Val Thr Val Ser Ala 
        115             

<210>  7
<211>  990
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗PD-L1抗体重链恒定区核苷酸序列


<400>  7
gcttcaacaa aaggaccttc cgtgtttcca ctggcaccct ctagtaagag tacttcagga        60
ggaaccgcag cactgggatg tctggtgaag gactacttcc cagagcccgt caccgtgtct       120
tggaacagtg gagcactgac ctccggggtc catacatttc ctgccgtgct gcagtcatcc       180
ggtctgtata gcctgagctc tgtggtcaca gtcccaagtt catccctggg cacccagaca       240
tacatctgca acgtgaatca caaaccttcc aatactaagg tcgacaagaa agtggaaccc       300
aagtcctgcg ataagaccca cacatgccct ccctgtcctg ctcccgaact gctgggagga       360
ccctccgtct tcctgttccc ccccaagccc aaagacacac tgatgatcag caggacccct       420
gaagtgacct gcgtggtcgt ggacgtgagc cacgaggacc ccgaggtcaa gtttaactgg       480
tacgtggacg gcgtggaggt ccacaacgcc aagaccaagc ccagggagga gcagtacgcc       540
agcacctaca gggtcgtgtc cgtgctgacc gtgctccacc aagattggct caacggcaag       600
gagtataagt gcaaagtcag caacaaggcc ctccccgccc ccatcgagaa aaccatcagc       660
aaggccaagg gccaaccgcg ggaacctcaa gtgtataccc tccctcccag ccgggatgag       720
ctgaccaaga accaagtctc cctcttgtgc ctggtcaagg gattctaccc ttccgacatt       780
gccgtcgaat gggagagcaa tggccagccc gagaacaact acaagacaac cccccccgtc       840
ctgcgcagcg acggatcctt cttcctgtac tccaagctca ccgtggacaa gagccggtgg       900
caacagggca acgtgttctc ctgtagcgtg atgcacgaag ccctccacaa ccactatacc       960
cagaagagcc tgagcctcag ccccggcaaa                                        990

<210>  8
<211>  330
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗PD-L1抗体重链恒定区氨基酸序列


<400>  8
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      
Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     
Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 
Gln Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 
Leu Thr Lys Asn Gln Val Ser Leu Leu Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         
Asn Tyr Lys Thr Thr Pro Pro Val Leu Arg Ser Asp Gly Ser Phe Phe 
        275                 280                 285             
Leu Tyr Ser Lys Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 

<210>  9
<211>  321
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人CD47抗体轻链可变区核苷酸序列


<400>  9
aacatccaga tgacccagtc cccttccgcc atgagcgctt ccgtgggcga cagggtgacc        60
atcacatgca aggcctccca ggacatccac cgttatttaa gctggtttca gcagaagccc       120
ggcaaggtgc ccaagcacct gatctacagg gctaacaggc tggtgtccgg cgtgccttcc       180
aggttttccg gcagcggttc tggcaccgag tttaccctga ccatctcctc cctgcagccc       240
gaggacttcg ccacctacta ctgcctgcag tacgacgagt tcccctacac cttcggcggc       300
ggcaccaagg tggagatcaa g                                                 321

<210>  10
<211>  107
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人CD47抗体轻链可变区氨基酸序列


<400>  10
Asn Ile Gln Met Thr Gln Ser Pro Ser Ala Met Ser Ala Ser Val Gly 
1               5                   10                  15      
Asp Arg Val Thr Ile Thr Cys Lys Ala Ser Gln Asp Ile His Arg Tyr 
            20                  25                  30          
Leu Ser Trp Phe Gln Gln Lys Pro Gly Lys Val Pro Lys His Leu Ile 
        35                  40                  45              
Tyr Arg Ala Asn Arg Leu Val Ser Gly Val Pro Ser Arg Phe Ser Gly 
    50                  55                  60                  
Ser Gly Ser Gly Thr Glu Phe Thr Leu Thr Ile Ser Ser Leu Gln Pro 
65                  70                  75                  80  
Glu Asp Phe Ala Thr Tyr Tyr Cys Leu Gln Tyr Asp Glu Phe Pro Tyr 
                85                  90                  95      
Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys 
            100                 105         

<210>  11
<211>  360
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人CD47抗体重链可变区核苷酸序列


<400>  11
cagatgcagc tggtgcagtc cggcgccgag gtgaaaaagc ccggctcctc cgtgaaggtg        60
tcctgcaagg ccagcggctt caacatcaag gactactacc tgcactgggt gaggcaggct       120
cccggacagg ctctggagtg gatgggctgg atcgaccccg accagggcga taccgagtac       180
gcccagaagt tccagggccg ggtgacaatc accagggaca ggagcacctc caccgcctac       240
atggagctga ggtccctgag gtccgaggac accgccgtgt actactgcaa cgccgcttac       300
ggcagcagct cctaccccat ggactactgg ggccagggca caaccgtgac cgtgagctcc       360

<210>  12
<211>  120
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人CD47抗体重链可变区氨基酸序列


<400>  12
Gln Met Gln Leu Val Gln Ser Gly Ala Glu Val Lys Lys Pro Gly Ser 
1               5                   10                  15      
Ser Val Lys Val Ser Cys Lys Ala Ser Gly Phe Asn Ile Lys Asp Tyr 
            20                  25                  30          
Tyr Leu His Trp Val Arg Gln Ala Pro Gly Gln Ala Leu Glu Trp Met 
        35                  40                  45              
Gly Trp Ile Asp Pro Asp Gln Gly Asp Thr Glu Tyr Ala Gln Lys Phe 
    50                  55                  60                  
Gln Gly Arg Val Thr Ile Thr Arg Asp Arg Ser Thr Ser Thr Ala Tyr 
65                  70                  75                  80  
Met Glu Leu Arg Ser Leu Arg Ser Glu Asp Thr Ala Val Tyr Tyr Cys 
                85                  90                  95      
Asn Ala Ala Tyr Gly Ser Ser Ser Tyr Pro Met Asp Tyr Trp Gly Gln 
            100                 105                 110         
Gly Thr Thr Val Thr Val Ser Ser 
        115                 120 

<210>  13
<211>  990
<212>  DNA
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人CD47抗体重链恒定区核苷酸序列


<400>  13
gctagcacaa aaggaccttc cgtgtttcca ctggcaccct ctagtaagag tacttcagga        60
ggaaccgcag cactgggatg tctggtgaag gactacttcc cagagcccgt caccgtgtct       120
tggaacagtg gagcactgac ctccggggtc catacatttc ctgccgtgct gcagtcatcc       180
ggtctgtata gcctgagctc tgtggtcaca gtcccaagtt catccctggg cacccagaca       240
tacatctgca acgtgaatca caaaccttcc aatactaagg tcgacaagaa agtggaaccc       300
aagtcctgcg ataagaccca cacatgccct ccctgtcctg ctcccgaact gctgggagga       360
ccctccgtct tcctgttccc ccccaagccc aaagacacac tgatgatcag caggacccct       420
gaagtgacct gcgtggtcgt ggacgtgagc cacgaggacc ccgaggtcaa gtttaactgg       480
tacgtggacg gcgtggaggt ccacaacgcc aagaccaagc ccagggagga gcagtacgcc       540
agcacctaca gggtggtcag cgtgctgacc gtgctgcacc aggattggct caacggcaag       600
gagtacaagt gcaaagtctc caacaaggcc ctgcccgccc ccatcgagaa gaccatctcc       660
aaggctaagg gacagcccag ggagccccaa gtgtacaccg agcctcccag ccgggatgag       720
ctgaccaaga accaagtctc cctcacctgc ctggtcaagg gattctaccc ttccgacatt       780
gccgtcgaat gggagagcaa tggccagccc gagaacaact acaagacaac cccccccgtc       840
ctggatagcg acggatcctt cttcctgctc tccgtgctca ccgtcgacaa gagcagatgg       900
cagcagggca acgtgttcag ctgtagcgtg atgcacgagg ccctgcacaa ccactacacc       960
cagaagagcc tgtccctcag ccccggcaag                                        990

<210>  14
<211>  330
<212>  PRT
<213>  人工序列(Artificial Sequence)

<220>
<223>  抗人CD47抗体重链恒定区氨基酸序列


<400>  14
Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Lys 
1               5                   10                  15      
Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          
Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              
Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  
Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  
Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      
Lys Val Glu Pro Lys Ser Cys Asp Lys Thr His Thr Cys Pro Pro Cys 
            100                 105                 110         
Pro Ala Pro Glu Leu Leu Gly Gly Pro Ser Val Phe Leu Phe Pro Pro 
        115                 120                 125             
Lys Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys 
    130                 135                 140                 
Val Val Val Asp Val Ser His Glu Asp Pro Glu Val Lys Phe Asn Trp 
145                 150                 155                 160 
Tyr Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu 
                165                 170                 175     
Glu Gln Tyr Ala Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu 
            180                 185                 190         
His Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn 
        195                 200                 205             
Lys Ala Leu Pro Ala Pro Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly 
    210                 215                 220                 
Gln Pro Arg Glu Pro Gln Val Tyr Thr Glu Pro Pro Ser Arg Asp Glu 
225                 230                 235                 240 
Leu Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr 
                245                 250                 255     
Pro Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn 
            260                 265                 270         
Asn Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe 
        275                 280                 285             
Leu Leu Ser Val Leu Thr Val Asp Lys Ser Arg Trp Gln Gln Gly Asn 
    290                 295                 300                 
Val Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr 
305                 310                 315                 320 
Gln Lys Ser Leu Ser Leu Ser Pro Gly Lys 
                325                 330 


