                         SEQUENCE LISTING

<110>  CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
 
<120>  Peptidase and its uses

<130>  BR86819

<160>  6     

<170>  PatentIn version 3.5

<210>  1
<211>  336
<212>  PRT
<213>  PYROCOCCUS HORIKOSHII

<400>  1

Met Glu Arg Ile Val Lys Ile Leu Arg Glu Ile Leu Glu Ile Pro Ser 
1               5                   10                  15      


Pro Thr Gly Tyr Thr Lys Glu Val Met Ser Tyr Leu Glu Lys Phe Leu 
            20                  25                  30          


Lys Glu Asn Glu Val Asn Phe Tyr Tyr Thr Asn Lys Gly Ala Leu Ile 
        35                  40                  45              


Ala Gly Asn His Pro Lys Pro Glu Leu Val Val Ile Ala His Val Asp 
    50                  55                  60                  


Thr Leu Gly Ala Met Val Lys Glu Ile Leu Pro Asp Gly His Leu Ala 
65                  70                  75                  80  


Phe Ser Arg Ile Gly Gly Leu Val Leu Pro Thr Phe Glu Gly Glu Tyr 
                85                  90                  95      


Cys Thr Ile Ile Thr Arg Lys Gly Lys Lys Phe Arg Gly Thr Leu Leu 
            100                 105                 110         


Leu Arg Asn Pro Ser Ala His Val Asn Arg Glu Val Gly Lys Lys Glu 
        115                 120                 125             


Arg Lys Glu Glu Asn Met Tyr Ile Arg Leu Asp Glu Leu Val Glu Lys 
    130                 135                 140                 


Arg Glu Asp Thr Glu Lys Leu Gly Ile Arg Pro Gly Asp Phe Ile Ala 
145                 150                 155                 160 


Phe Asp Pro Lys Phe Glu Tyr Val Asn Gly Phe Val Lys Ser His Phe 
                165                 170                 175     


Leu Asp Asp Lys Ala Ser Val Ala Ala Ile Leu Asp Leu Ile Ile Asp 
            180                 185                 190         


Met Lys Asp Glu Leu Glu Lys Tyr Pro Val Ala Phe Phe Phe Ser Pro 
        195                 200                 205             


Tyr Glu Glu Val Gly His Gly Gly Ser Ala Gly Tyr Pro Pro Thr Thr 
    210                 215                 220                 


Lys Glu Leu Leu Val Val Asp Met Gly Val Val Gly Glu Gly Val Ser 
225                 230                 235                 240 


Gly Lys Glu Thr Ala Val Ser Ile Ala Ala Lys Asp Thr Thr Gly Pro 
                245                 250                 255     


Tyr Asp Tyr Asp Met Thr Asn Arg Leu Ile Glu Leu Ala Glu Glu Asn 
            260                 265                 270         


Asn Ile Pro Tyr Val Val Asp Val Phe Pro Tyr Tyr Gly Ser Asp Gly 
        275                 280                 285             


Ser Ala Ala Leu Arg Ala Gly Trp Asp Phe Arg Val Ala Leu Ile Gly 
    290                 295                 300                 


Pro Gly Val His Ala Ser His Gly Met Glu Arg Thr His Val Lys Gly 
305                 310                 315                 320 


Leu Leu Ala Thr Lys Glu Leu Ile Arg Ala Tyr Ile Lys Trp Lys Gly 
                325                 330                 335     


<210>  2
<211>  332
<212>  PRT
<213>  PYROCOCCUS HORIKOSHII

<400>  2

Met Met Ser Met Ile Glu Lys Leu Lys Lys Phe Thr Gln Ile Pro Gly 
1               5                   10                  15      


Ile Ser Gly Tyr Glu Glu Arg Ile Arg Glu Glu Ile Ile Arg Glu Ile 
            20                  25                  30          


Lys Asp Phe Ala Asp Tyr Lys Val Asp Ala Ile Gly Asn Leu Ile Val 
        35                  40                  45              


Glu Leu Gly Glu Gly Glu Glu Arg Ile Leu Phe Met Ala His Met Asp 
    50                  55                  60                  


Glu Ile Gly Leu Leu Ile Thr Gly Ile Thr Asp Glu Gly Lys Leu Arg 
65                  70                  75                  80  


Phe Arg Lys Val Gly Gly Ile Asp Asp Arg Leu Leu Tyr Gly Arg His 
                85                  90                  95      


Val Asn Val Val Thr Glu Lys Gly Ile Leu Asp Gly Val Ile Gly Ala 
            100                 105                 110         


Thr Pro Pro His Leu Ser Leu Glu Arg Asp Lys Ser Val Ile Pro Trp 
        115                 120                 125             


Tyr Asp Leu Val Ile Asp Ile Gly Ala Glu Ser Lys Glu Glu Ala Leu 
    130                 135                 140                 


Glu Leu Val Lys Pro Leu Asp Phe Ala Val Phe Lys Lys His Phe Ser 
145                 150                 155                 160 


Val Leu Asn Gly Lys Tyr Val Ser Thr Arg Gly Leu Asp Asp Arg Phe 
                165                 170                 175     


Gly Val Val Ala Leu Ile Glu Ala Ile Lys Asp Leu Val Asp His Glu 
            180                 185                 190         


Leu Glu Gly Lys Val Ile Phe Ala Phe Thr Val Gln Glu Glu Val Gly 
        195                 200                 205             


Leu Lys Gly Ala Lys Phe Leu Ala Asn His Tyr Tyr Pro Gln Tyr Ala 
    210                 215                 220                 


Phe Ala Ile Asp Ser Phe Ala Cys Cys Ser Pro Leu Thr Gly Asp Val 
225                 230                 235                 240 


Lys Leu Gly Lys Gly Pro Val Ile Arg Ala Val Asp Asn Ser Ala Ile 
                245                 250                 255     


Tyr Ser Arg Asp Leu Ala Arg Lys Val Trp Ser Ile Ala Glu Lys Asn 
            260                 265                 270         


Gly Ile Glu Ile Gln Ile Gly Val Thr Gly Gly Gly Thr Asp Ala Ser 
        275                 280                 285             


Ala Phe Gln Asp Arg Ser Lys Thr Leu Ala Leu Ser Val Pro Ile Lys 
    290                 295                 300                 


Tyr Leu His Ser Glu Val Glu Thr Leu His Leu Asn Asp Leu Glu Lys 
305                 310                 315                 320 


Leu Val Lys Leu Ile Glu Ala Leu Ala Phe Glu Leu 
                325                 330         


<210>  3
<211>  353
<212>  PRT
<213>  PYROCOCCUS HORIKOSHII

<400>  3

Met Glu Val Arg Asn Met Val Asp Tyr Glu Leu Leu Lys Lys Val Val 
1               5                   10                  15      


Glu Ala Pro Gly Val Ser Gly Tyr Glu Phe Leu Gly Ile Arg Asp Val 
            20                  25                  30          


Val Ile Glu Glu Ile Lys Asp Tyr Val Asp Glu Val Lys Val Asp Lys 
        35                  40                  45              


Leu Gly Asn Val Ile Ala His Lys Lys Gly Glu Gly Pro Lys Val Met 
    50                  55                  60                  


Ile Ala Ala His Met Asp Gln Ile Gly Leu Met Val Thr His Ile Glu 
65                  70                  75                  80  


Lys Asn Gly Phe Leu Arg Val Ala Pro Ile Gly Gly Val Asp Pro Lys 
                85                  90                  95      


Thr Leu Ile Ala Gln Arg Phe Lys Val Trp Ile Asp Lys Gly Lys Phe 
            100                 105                 110         


Ile Tyr Gly Val Gly Ala Ser Val Pro Pro His Ile Gln Lys Pro Glu 
        115                 120                 125             


Asp Arg Lys Lys Ala Pro Asp Trp Asp Gln Ile Phe Ile Asp Ile Gly 
    130                 135                 140                 


Ala Glu Ser Lys Glu Glu Ala Glu Asp Met Gly Val Lys Ile Gly Thr 
145                 150                 155                 160 


Val Ile Thr Trp Asp Gly Arg Leu Glu Arg Leu Gly Lys His Arg Phe 
                165                 170                 175     


Val Ser Ile Ala Phe Asp Asp Arg Ile Ala Val Tyr Thr Ile Leu Glu 
            180                 185                 190         


Val Ala Lys Gln Leu Lys Asp Ala Lys Ala Asp Val Tyr Phe Val Ala 
        195                 200                 205             


Thr Val Gln Glu Glu Val Gly Leu Arg Gly Ala Arg Thr Ser Ala Phe 
    210                 215                 220                 


Gly Ile Glu Pro Asp Tyr Gly Phe Ala Ile Asp Val Thr Ile Ala Ala 
225                 230                 235                 240 


Asp Ile Pro Gly Thr Pro Glu His Lys Gln Val Thr His Leu Gly Lys 
                245                 250                 255     


Gly Thr Ala Ile Lys Ile Met Asp Arg Ser Val Ile Cys His Pro Thr 
            260                 265                 270         


Ile Val Arg Trp Leu Glu Glu Leu Ala Lys Lys His Glu Ile Pro Tyr 
        275                 280                 285             


Gln Leu Glu Ile Leu Leu Gly Gly Gly Thr Asp Ala Gly Ala Ile His 
    290                 295                 300                 


Leu Thr Lys Ala Gly Val Pro Thr Gly Ala Leu Ser Val Pro Ala Arg 
305                 310                 315                 320 


Tyr Ile His Ser Asn Thr Glu Val Val Asp Glu Arg Asp Val Asp Ala 
                325                 330                 335     


Thr Val Glu Leu Met Thr Lys Ala Leu Glu Asn Ile His Glu Leu Lys 
            340                 345                 350         


Ile 
    


<210>  4
<211>  354
<212>  PRT
<213>  PYROCOCCUS HORIKOSHII

<400>  4

Met Asp Leu Lys Gly Gly Glu Ser Met Val Asp Trp Lys Leu Met Gln 
1               5                   10                  15      


Glu Ile Ile Glu Ala Pro Gly Val Ser Gly Tyr Glu His Leu Gly Ile 
            20                  25                  30          


Arg Asp Ile Val Val Asp Val Leu Lys Glu Val Ala Asp Glu Val Lys 
        35                  40                  45              


Val Asp Lys Leu Gly Asn Val Ile Ala His Phe Lys Gly Ser Ser Pro 
    50                  55                  60                  


Arg Ile Met Val Ala Ala His Met Asp Lys Ile Gly Val Met Val Asn 
65                  70                  75                  80  


His Ile Asp Lys Asp Gly Tyr Leu His Ile Val Pro Ile Gly Gly Val 
                85                  90                  95      


Leu Pro Glu Thr Leu Val Ala Gln Arg Ile Arg Phe Phe Thr Glu Lys 
            100                 105                 110         


Gly Glu Arg Tyr Gly Val Val Gly Val Leu Pro Pro His Leu Arg Arg 
        115                 120                 125             


Gly Gln Glu Asp Lys Gly Ser Lys Ile Asp Trp Asp Gln Ile Val Val 
    130                 135                 140                 


Asp Val Gly Ala Ser Ser Lys Glu Glu Ala Glu Glu Met Gly Phe Arg 
145                 150                 155                 160 


Val Gly Thr Val Gly Glu Phe Ala Pro Asn Phe Thr Arg Leu Asn Glu 
                165                 170                 175     


His Arg Phe Ala Thr Pro Tyr Leu Asp Asp Arg Ile Cys Leu Tyr Ala 
            180                 185                 190         


Met Ile Glu Ala Ala Arg Gln Leu Gly Asp His Glu Ala Asp Ile Tyr 
        195                 200                 205             


Ile Val Gly Ser Val Gln Glu Glu Val Gly Leu Arg Gly Ala Arg Val 
    210                 215                 220                 


Ala Ser Tyr Ala Ile Asn Pro Glu Val Gly Ile Ala Met Asp Val Thr 
225                 230                 235                 240 


Phe Ala Lys Gln Pro His Asp Lys Gly Lys Ile Val Pro Glu Leu Gly 
                245                 250                 255     


Lys Gly Pro Val Met Asp Val Gly Pro Asn Ile Asn Pro Lys Leu Arg 
            260                 265                 270         


Ala Phe Ala Asp Glu Val Ala Lys Lys Tyr Glu Ile Pro Leu Gln Val 
        275                 280                 285             


Glu Pro Ser Pro Arg Pro Thr Gly Thr Asp Ala Asn Val Met Gln Ile 
    290                 295                 300                 


Asn Lys Glu Gly Val Ala Thr Ala Val Leu Ser Ile Pro Ile Arg Tyr 
305                 310                 315                 320 


Met His Ser Gln Val Glu Leu Ala Asp Ala Arg Asp Val Asp Asn Thr 
                325                 330                 335     


Ile Lys Leu Ala Lys Ala Leu Leu Glu Glu Leu Lys Pro Met Asp Phe 
            340                 345                 350         


Thr Pro 
        


<210>  5
<211>  1011
<212>  DNA
<213>  PYROCOCCUS HORIKOSHII

<400>  5
atggaaagga tagtcaagat cttaagggaa atcttagaga taccttctcc aacgggctac       60

acgaaggagg taatgagtta cctagaaaaa tttctaaagg aaaatgaagt aaacttttac      120

tatacgaaca agggggccct aatagccggt aatcatccaa agcctgagct cgttgttata      180

gcccacgtag acacgcttgg ggcaatggtt aaggagatac taccagacgg acacttagca      240

ttttcaagga taggagggct cgttctacct acgtttgaag gcgaatactg tactataata      300

acgagaaaag gaaagaagtt tagaggaacg ctcctcctta gaaatccgag cgctcatgta      360

aatagggaag taggtaaaaa ggagagaaaa gaggagaata tgtatataag attggacgag      420

ctcgtggaga agagagagga tacagaaaag ctggggataa ggccaggaga cttcatagct      480

tttgatccca aatttgaata cgtaaacggc tttgttaaat cacacttcct agatgacaag      540

gctagcgtag ctgcaatact cgatctaata atagatatga aggatgaact cgagaagtat      600

ccagttgcat tcttcttctc accgtatgag gaagttggcc acggaggctc agctggctac      660

ccaccaacga ctaaggaact gctcgtggtt gatatgggag tagtgggtga aggtgtttca      720

ggaaaagaaa ccgccgtatc tatagcggcc aaggatacaa ctgggcctta tgactatgac      780

atgacgaaca ggttaataga gcttgctgaa gagaacaata tcccatatgt agttgacgtg      840

ttcccctact atggttccga tggttcagct gcactaagag ctggatggga tttcagggtt      900

gccctaattg ggccaggtgt gcacgcaagc cacggaatgg agagaaccca cgttaaggga      960

ttgttggcaa ctaaagagct tataagggct tacataaaat ggaaggggta a              1011


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Generated peptide for amidolytic activity assay

<400>  6

Gly Met Asp Ser Leu Ala Phe Ser Gly Gly Leu 
1               5                   10      


