<110>    FarmHannong Co., Ltd.

<120>    Methods and Compositions for Conferring and/or Enhancing
         Herbicide Tolerance Using Variants of Protoporphyrinogen Oxidase
         from Cyanobacteria

<130>    OPP20183938KR

<150>    10-2017-0173633
<151>    2017-12-15

<160>    90

<170>    KopatentIn 3.0

<210>    1
<211>    462
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic (Protoporphyrinogen IX oxidase (CyPPO16) of Spirulina
         subsalsa)


<400>    1
Met Leu Asp Ser Leu Ile Val Gly Ala Gly Ile Ser Gly Leu Ser Ala
  1               5                  10                  15 

Ala His Thr Leu Gln Lys Gln Gln Thr Gln Phe Leu Val Thr Glu Ser
             20                  25                  30 

Gln Gly Arg Val Gly Gly Asn Ile Thr Thr Asn Arg Gln Gly Asp Tyr
         35                  40                  45 

Leu Trp Glu Glu Gly Pro Asn Ser Phe Ala Pro Thr Glu Asp Leu Leu
     50                  55                  60 

Arg Leu Ala Val Glu Val Gly Leu Lys Glu Asp Leu Val Phe Ala Asp
 65                  70                  75                  80 

Arg Arg Leu Pro Arg Phe Val Tyr Trp Asn Gln Gln Leu His Pro Val
                 85                  90                  95 

Pro Met Ser Pro Pro Ala Ala Leu Lys Thr Gln Leu Leu Ser Glu Ala
            100                 105                 110 

Gly Lys Trp Arg Ala Ala Leu Gly Ala Leu Gly Phe Val Gly Gly Leu
        115                 120                 125 

Val Gly Arg Glu Glu Glu Thr Val Arg Gln Phe Phe Thr Arg His Leu
    130                 135                 140 

Gly Thr Glu Val Thr Glu Arg Leu Val Ala Pro Phe Val Ser Gly Val
145                 150                 155                 160 

Tyr Ala Gly Asp Val Asp Gln Leu Ser Ala Gln Ala Ala Phe Arg Arg
                165                 170                 175 

Val Phe Glu Phe Ala Gln Leu Gly Gly Gly Leu Ala Ala Gly Gly Ile
            180                 185                 190 

Leu Ala Arg Arg Gln Ala Pro Pro Lys Ala Pro Pro Asp Pro Ser Leu
        195                 200                 205 

Pro Glu Thr Lys Thr Gly Gln Leu Gly Ser Phe Arg Glu Gly Leu Glu
    210                 215                 220 

Met Leu Pro Arg Ala Ile Ala Ser Gln Leu Gly Asp Arg Leu Lys Leu
225                 230                 235                 240 

Gln Trp Arg Leu Thr His Leu Glu Ile Thr Pro Gln Gln Thr Tyr Leu
                245                 250                 255 

Ala His Phe Asn Thr Pro Asp Gly Pro Gln Gln Ile Ala Thr Arg Thr
            260                 265                 270 

Leu Ile Leu Thr Thr Pro Ala Pro Ile Thr Ala Asp Leu Leu Lys Pro
        275                 280                 285 

Leu Thr Pro Ala Leu His Gly Val Leu Lys Glu Ile Tyr Tyr Pro Pro
    290                 295                 300 

Val Ala Cys Val Val Leu Ala Tyr Pro Arg Ala Ala Ser Ala Arg Pro
305                 310                 315                 320 

Leu Glu Gly Phe Gly His Leu Ile Pro Arg Asn Gln Gly Ile Arg Thr
                325                 330                 335 

Leu Gly Thr Ile Trp Ser Ser Cys Leu Phe Pro Gly Arg Thr Pro Glu
            340                 345                 350 

Gly Glu His Leu Leu Thr Asn Phe Ile Gly Gly Ala Thr Asp Pro Gly
        355                 360                 365 

Ile Ala Gln Leu Asp Pro Glu Glu Ile Ala Gln Ala Val His Gln Asp
    370                 375                 380 

Leu Cys Lys Ile Leu Ile Arg Pro Glu Phe Thr Pro Lys Ile Leu Ala
385                 390                 395                 400 

Val Arg Leu Trp Lys Gln Ala Ile Pro Gln Tyr Thr Leu Gly His Leu
                405                 410                 415 

Gln Arg Leu Ala Thr Leu Glu Gln Glu Leu Ser Lys Phe Pro Gly Leu
            420                 425                 430 

His Ile Leu Ala Asn Tyr Thr Asp Gly Val Ala Leu Gly Asp Cys Val
        435                 440                 445 

Lys Arg Gly Val Ala Val Ala Gln Lys Ile Arg Ser Arg Glu
    450                 455                 460 




<210>    2
<211>    1389
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO16 coding gene from Spirulina subsalsa)


<400>    2
atgctagact ccctgattgt aggggccgga atatcaggac tctcagctgc acacaccctt         60

cagaagcagc agactcagtt tctggtcacg gaatcccaag ggcgtgtggg tggcaatata        120

acgacgaacc gtcaaggtga ttacttgtgg gaggaaggtc ccaattcttt tgcgccaaca        180

gaggaccttt tacgacttgc tgtcgaggta gggctcaaag aggatctggt attcgccgac        240

agacgtctac cgaggtttgt gtattggaac caacagttac accctgtacc catgagccct        300

cctgcggcgt tgaagactca actactgtca gaagctggaa agtggagagc agcattgggg        360

gcgctgggat ttgtaggcgg ccttgtcgga agagaggaag aaacggttcg acaatttttt        420

acgcgtcatc ttggtacgga agttaccgag cgtttagtcg caccatttgt ctccggtgtg        480

tatgctggcg acgttgatca actatccgcg caagcggcgt ttcgtagagt ctttgagttc        540

gcacagctcg gcggggggtt ggccgcgggg gggattcttg cgcgtcgtca ggccccccca        600

aaagccccac ccgatcccag tttacccgaa accaagactg ggcagttagg ctcttttcgt        660

gaagggctag aaatgctccc cagagcaata gccagccaac tcggagacag gttaaagcta        720

cagtggcgac taacgcatct ggagataact ccccaacaaa cttatttggc gcattttaat        780

actccggatg gcccgcagca aatcgcaaca aggactctga tcctaacaac ccccgctcct        840

atcactgctg atcttcttaa acctttgacg cccgcgcttc atggggtgct caaggaaatt        900

tactatcctc cggtagcctg cgtagtccta gcatacccaa gggccgcgag tgcgcgtcca        960

ttggaaggtt ttggtcatct tatacccagg aatcagggga taaggactct tggtacaatc       1020

tggtcctcct gtctcttccc gggcaggacg cctgagggtg agcatctgct gaccaatttc       1080

atcggcggcg caacggaccc tggtatagcc cagctagatc ctgaggaaat cgctcaagcc       1140

gtccatcaag atttgtgcaa gatactgatc agaccagaat tcactcccaa aatcttagcc       1200

gtcaggctgt ggaagcaagc tattcctcag tatacgctag ggcacttgca gcgacttgca       1260

accttggaac aggagttgtc caagtttcct gggcttcata tcctagctaa ttatacggat       1320

ggtgttgcgc tcggcgattg cgtcaaacgt ggagttgcag tggctcaaaa aattagaagc       1380

agggagtga                                                               1389


<210>    3
<211>    466
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic (Protoporphyrinogen IX oxidase (CyPPO17) of
         Thermosynechococcus sp. NK55a)


<400>    3
Met Glu Val Asp Val Ala Ile Val Gly Gly Gly Leu Ser Gly Leu Ser
  1               5                  10                  15 

Val Ala Trp Arg Leu Gln Gln Ser Ala Pro Gln Tyr Ser Gly Val Leu
             20                  25                  30 

Leu Glu Ala Ser Asp Arg Leu Gly Gly Asn Ile Thr Thr Gln Gly Ala
         35                  40                  45 

Glu Gly Phe Val Trp Glu Leu Gly Pro Asn Ser Phe Ala Pro Thr Pro
     50                  55                  60 

Ala Leu Leu Gln Leu Ile Ala Glu Val Gly Leu His Ser Glu Leu Ile
 65                  70                  75                  80 

Arg Gly Asp Arg His Leu Pro Arg Tyr Ile Tyr Trp Arg Gly Glu Leu
                 85                  90                  95 

Tyr Pro Leu Glu Pro Thr Arg Pro Leu Ala Leu Ala Thr Ser Asn Leu
            100                 105                 110 

Leu Ser Pro Trp Gly Lys Val Arg Ala Ala Leu Gly Ala Leu Gly Phe
        115                 120                 125 

Val Pro Pro Tyr Leu Gly Ser Gly Asp Glu Ser Val Asn Ser Phe Phe
    130                 135                 140 

Arg Arg His Leu Gly Gln Glu Val Ala Glu Arg Leu Val Ala Pro Phe
145                 150                 155                 160 

Val Ser Gly Val Tyr Ala Gly Asp Pro Gln Gln Leu Ser Ala Ala Ala
                165                 170                 175 

Ala Phe Arg Arg Ile Ala Gln Leu Glu Lys Leu Gly Gly Gly Leu Ile
            180                 185                 190 

Ala Gly Ala Leu Arg Leu Arg Arg Gln Gln Pro Pro Lys Pro Arg Pro
        195                 200                 205 

Pro Ala Glu Val Gln Met Arg Pro Gly Glu Leu Gly Ser Phe Lys Glu
    210                 215                 220 

Gly Leu Ala Ala Leu Pro Arg Ala Ile Ala Gln Gln Leu Lys Ala Pro
225                 230                 235                 240 

Val His Leu Gln Thr Pro Val Glu Ala Ile Thr Pro Glu Pro Asn Gly
                245                 250                 255 

Gly Tyr Leu Leu Arg Ser Gly Glu Gln Thr Trp Gln Ala Arg Ser Val
            260                 265                 270 

Val Leu Ala Thr Pro Ala Tyr Gln Thr Ala Ala Leu Val Ala Pro Phe
        275                 280                 285 

Gln Pro Ala Ile Ala Arg Val Leu Ala Ala Ile Pro Tyr Pro Thr Val
    290                 295                 300 

Ala Cys Val Val Leu Ala Tyr Pro Ala Gly Leu Gly Arg Ser Val Arg
305                 310                 315                 320 

Pro Gly Phe Gly Val Leu Ile Pro Arg Gly Gln Gly Ile Arg Thr Leu
                325                 330                 335 

Gly Thr Ile Trp Ser Ser Cys Leu Phe Pro Gln Arg Thr Pro Ala Gly
            340                 345                 350 

Trp Gln Val Phe Thr Ser Phe Ile Gly Gly Ala Thr Asp Pro Asp Leu
        355                 360                 365 

Ala Ser Leu Arg Glu Glu Ala Ile Val Gln Gln Val Gln Gln Asp Leu
    370                 375                 380 

Thr Arg Leu Leu Asp Leu Pro Ala Ala Lys Ala Arg Leu Leu Gly Met
385                 390                 395                 400 

Lys Val Trp Arg Arg Ala Ile Pro Gln Tyr Leu Val Gly Tyr Pro Gln
                405                 410                 415 

Gln Trp Gln Gln Val Thr His Ala Leu Ser Gln Thr Pro Gly Leu Phe
            420                 425                 430 

Leu Cys Ser Asn Tyr Ala Glu Gly Val Ala Leu Gly Asp Arg Val Glu
        435                 440                 445 

His Gly Asn Arg Thr Ala Ala Ala Val Ala Ala Tyr Leu Ser Gly Gly
    450                 455                 460 

Gln Ser
465    


<210>    4
<211>    1401
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO17 coding gene from Thermosynechococcus sp.
         NK55a)


<400>    4
atggaggtcg atgttgcaat tgtcggcggc ggcctgtcag gcttgagcgt tgcctggcgt         60

ctccagcagt ctgctcctca atacagtggc gttctattgg aagcgtcaga ccgtctaggt        120

gggaacatta ctacgcaagg ggcagagggg ttcgtgtggg aactaggccc taacagcttc        180

gctccgactc ccgcgctttt acagcttata gcagaagtag ggcttcactc cgagcttata        240

cgtggcgatc gacatctacc gcgatacatt tattggcgag gcgaacttta tccattggag        300

ccgaccaggc cgcttgcttt ggcaacaagc aatctcctct ctccatgggg taaggtcaga        360

gcagcgttgg gcgccctcgg gtttgtcccc ccgtatctcg gatctggcga tgaatccgtt        420

aattcatttt tccgacgaca cctaggtcag gaggtagccg aacgtctggt ggcacctttc        480

gtctctggcg tgtatgcggg agatccccag caactgtccg ccgccgctgc atttagacga        540

atagcgcagc tggaaaaact aggtggggga ctcatagcag gagcattgag gctgaggaga        600

cagcaacccc ccaaacccag gcccccggca gaggttcaaa tgcgacccgg tgagttgggg        660

tctttcaaag agggattggc cgccttgcca cgtgcaatag ctcaacagct gaaagcacca        720

gtgcacctac aaaccccagt cgaagcaatc acgccggagc ccaacggagg ttatctactc        780

agatccggcg aacagacttg gcaagcgaga agcgtcgtcc tagcaacacc tgcctatcag        840

accgcagcat tagtggcgcc gtttcaacct gcaatagcgc gagtgttagc ggccattccg        900

tatccaacag tggcctgtgt agtactcgcc tatcccgcgg gactaggcag gtcagtacga        960

ccaggctttg gcgtccttat accccgtggc caaggtatcc gtacactcgg cactatatgg       1020

tctagctgct tatttccaca gcgaactcca gctgggtggc aagtttttac ttcattcatc       1080

ggtggagcaa cagatccgga cctcgcctca ctgagggaag aagcgatcgt tcaacaggtg       1140

caacaggact tgacccgtct acttgatctt cctgccgcca aggcgagact cctcggtatg       1200

aaagtgtggc gaagggcgat cccacaatac ttggtagggt atccacagca atggcagcaa       1260

gtaacccatg cccttagtca gacgccgggc cttttcctgt gtagtaacta cgccgagggc       1320

gtggcattag gcgacagggt tgaacatggt aacaggaccg ctgcggcagt ggccgcgtac       1380

ctgagtgggg ggcaatcctg a                                                 1401


<210>    5
<211>    467
<212>    PRT
<213>    Artificial Sequence

<220>
<223>    Synthetic (Protoporphyrinogen IX oxidase (CyPPO10) of
         Thermosynechococcus elongatus BP-1)


<400>    5
Met Ile Glu Val Asp Val Ala Ile Val Gly Gly Gly Leu Ser Gly Leu
  1               5                  10                  15 

Ser Val Ala Trp Arg Leu Gln Arg Ser Ala Pro His Tyr Ser Gly Val
             20                  25                  30 

Leu Leu Glu Ala Ser Asp Arg Leu Gly Gly Asn Ile Thr Thr Gln Ala
         35                  40                  45 

Ala Glu Gly Phe Val Trp Glu Leu Gly Pro Asn Ser Phe Ala Pro Thr
     50                  55                  60 

Pro Ala Leu Leu Gln Leu Ile Ala Glu Val Gly Leu His Ser Glu Leu
 65                  70                  75                  80 

Ile Arg Gly Asp Arg His Leu Pro Arg Tyr Ile Tyr Trp Arg Gly Glu
                 85                  90                  95 

Leu Tyr Pro Leu Glu Pro Thr Arg Pro Leu Ala Leu Ala Thr Ser Asn
            100                 105                 110 

Leu Leu Ser Pro Trp Gly Lys Val Arg Ala Ala Leu Gly Ala Leu Gly
        115                 120                 125 

Phe Val Pro Pro Tyr Leu Gly Ser Gly Asp Glu Ser Val Asp Ser Phe
    130                 135                 140 

Phe Arg Arg His Leu Gly Gln Glu Val Ala Glu Arg Leu Val Ala Pro
145                 150                 155                 160 

Phe Val Ser Gly Val Tyr Ala Gly Asp Pro Gln Gln Leu Ser Ala Ala
                165                 170                 175 

Ala Ala Phe Arg Arg Ile Ala Gln Leu Glu Lys Leu Gly Gly Ser Leu
            180                 185                 190 

Ile Ala Gly Ala Leu Arg Leu Arg Arg Gln Gln Pro Pro Gln Pro Lys
        195                 200                 205 

Pro Pro Ala Gln Val Gln Met Arg Pro Gly Glu Leu Gly Ser Phe Arg
    210                 215                 220 

Glu Gly Leu Ala Ala Leu Pro Arg Ala Ile Ala Gln Gln Leu Lys Ala
225                 230                 235                 240 

Pro Leu His Leu Gln Thr Pro Val Glu Ala Ile Thr Pro Glu Pro Lys
                245                 250                 255 

Gly Gly Tyr Leu Leu Arg Ser Gly Glu Gln Thr Trp His Ala Arg Ser
            260                 265                 270 

Val Val Leu Ala Thr Pro Ala Tyr Gln Thr Ala Glu Leu Val Ala Pro
        275                 280                 285 

Phe Gln Pro Ala Ile Ala Arg Ala Leu Ala Thr Ile Pro Tyr Pro Thr
    290                 295                 300 

Val Ala Cys Val Val Leu Ala Tyr Pro Ala Gly Leu Gly Arg Ser Val
305                 310                 315                 320 

Arg Pro Gly Phe Gly Val Leu Val Pro Arg Gly Gln Gly Ile Arg Thr
                325                 330                 335 

Leu Gly Thr Ile Trp Ser Ser Cys Leu Phe Pro Gln Arg Thr Pro Ala
            340                 345                 350 

Gly Trp Gln Val Phe Thr Ser Phe Ile Gly Gly Ala Thr Asp Pro Asp
        355                 360                 365 

Leu Ala Ser Leu Arg Glu Glu Ala Ile Val Glu Gln Val Gln Gln Asp
    370                 375                 380 

Leu Thr Arg Leu Leu Asp Leu Pro Ala Ala Lys Ala Arg Leu Leu Gly
385                 390                 395                 400 

Met Lys Val Trp Arg Arg Ala Ile Pro Gln Tyr Ile Val Gly Tyr Pro
                405                 410                 415 

Gln Gln Trp Gln Gln Val Thr His Ala Leu Thr Gln Thr Pro Gly Leu
            420                 425                 430 

Phe Leu Cys Ser Asn Tyr Ala Glu Gly Val Ala Leu Gly Asp Arg Val
        435                 440                 445 

Glu His Gly Asn Arg Thr Ala Ala Ala Val Ala Ala Tyr Leu Ala Gly
    450                 455                 460 

Gly Gln Ser
465        


<210>    6
<211>    1404
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO10 coding gene)


<400>    6
atgattgaag tggatgtggc tattgttggt ggtggtctta gtggattgtc agtggcttgg         60

agattacaga ggagtgctcc tcattattct ggagttcttc ttgaggcttc tgatagactt        120

ggaggtaata tcactacaca agctgctgaa ggatttgtgt gggagcttgg tccaaacagt        180

ttcgctccta ctccagcact cttacagttg attgctgaag ttggactcca ttctgagtta        240

atcagaggag ataggcacct tccaagatat atatactgga ggggagaact ttatcctttg        300

gagccaacta ggcctcttgc tttggcaaca tcaaatcttt tgagtccttg gggaaaggtt        360

agagctgcac tcggagcttt aggttttgtg cctccatatc ttggatctgg agatgaaagt        420

gttgattctt tctttagaag gcatcttgga caagaagttg ctgagagatt ggtggcacca        480

tttgtttcag gagtgtacgc tggagatcct caacagcttt ctgctgctgc tgcttttaga        540

aggattgctc aacttgagaa gttgggaggt tcattgatcg ctggagcact cagattaaga        600

aggcaacagc ctccacagcc aaaacctcca gctcaagtgc agatgagacc tggagaactc        660

ggtagtttta gggagggtct cgctgcatta cctagagcta tcgcacaaca gttgaaggca        720

ccacttcatt tgcaaacacc tgttgaagct attacccctg agccaaaagg aggttatctc        780

ttaaggagtg gtgaacagac ttggcacgct agatcagttg tgttggctac tccagcatac        840

caaactgctg aacttgttgc accattccag cctgctatcg ctagagcttt ggctaccata        900

ccttatccaa ctgttgcttg tgttgtgctt gcttaccctg ctggattggg tagatcagtt        960

agacctggat ttggtgtttt ggtgcctaga ggacaaggta taaggacact cggaaccatt       1020

tggtcttcat gcttattccc acaaagaact cctgctggtt ggcaggtttt tacctctttc       1080

ataggaggtg ctactgatcc tgatcttgca tcattgagag aagaggctat tgttgaacaa       1140

gtgcaacagg atctcacaag gcttcttgat cttcctgctg caaaggcaag actcttgggt       1200

atgaaggttt ggagaagggc tattccacaa tatatcgttg gttaccctca acagtggcaa       1260

caggtgacac acgctcttac ccagactcct ggtctcttct tatgttcaaa ctacgctgag       1320

ggagttgcat tgggagatag agtggaacac ggaaatagga ctgctgctgc tgtggctgct       1380

tacctcgctg gtggacaatc ataa                                              1404


<210>    7
<211>    1389
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (Optimized codon encoding CyPPO16)


<400>    7
atgctagact ccctgattgt aggggccgga atatcaggac tctcagctgc acacaccctt         60

cagaagcagc agactcagtt tctggtcacg gaatcccaag ggcgtgtggg tggcaatata        120

acgacgaacc gtcaaggtga ttacttgtgg gaggaaggtc ccaattcttt tgcgccaaca        180

gaggaccttt tacgacttgc tgtcgaggta gggctcaaag aggatctggt attcgccgac        240

agacgtctac cgaggtttgt gtattggaac caacagttac accctgtacc catgagccct        300

cctgcggcgt tgaagactca actactgtca gaagctggaa agtggagagc agcattgggg        360

gcgctgggat ttgtaggcgg ccttgtcgga agagaggaag aaacggttcg acaatttttt        420

acgcgtcatc ttggtacgga agttaccgag cgtttagtcg caccatttgt ctccggtgtg        480

tatgctggcg acgttgatca actatccgcg caagcggcgt ttcgtagagt ctttgagttc        540

gcacagctcg gcggggggtt ggccgcgggg gggattcttg cgcgtcgtca ggccccccca        600

aaagccccac ccgatcccag tttacccgaa accaagactg ggcagttagg ctcttttcgt        660

gaagggctag aaatgctccc cagagcaata gccagccaac tcggagacag gttaaagcta        720

cagtggcgac taacgcatct ggagataact ccccaacaaa cttatttggc gcattttaat        780

actccggatg gcccgcagca aatcgcaaca aggactctga tcctaacaac ccccgctcct        840

atcactgctg atcttcttaa acctttgacg cccgcgcttc atggggtgct caaggaaatt        900

tactatcctc cggtagcctg cgtagtccta gcatacccaa gggccgcgag tgcgcgtcca        960

ttggaaggtt ttggtcatct tatacccagg aatcagggga taaggactct tggtacaatc       1020

tggtcctcct gtctcttccc gggcaggacg cctgagggtg agcatctgct gaccaatttc       1080

atcggcggcg caacggaccc tggtatagcc cagctagatc ctgaggaaat cgctcaagcc       1140

gtccatcaag atttgtgcaa gatactgatc agaccagaat tcactcccaa aatcttagcc       1200

gtcaggctgt ggaagcaagc tattcctcag tatacgctag ggcacttgca gcgacttgca       1260

accttggaac aggagttgtc caagtttcct gggcttcata tcctagctaa ttatacggat       1320

ggtgttgcgc tcggcgattg cgtcaaacgt ggagttgcag tggctcaaaa aattagaagc       1380

agggagtga                                                               1389


<210>    8
<211>    1401
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (Optimized codon encoding CyPPO17)


<400>    8
atggaggtcg atgttgcaat tgtcggcggc ggcctgtcag gcttgagcgt tgcctggcgt         60

ctccagcagt ctgctcctca atacagtggc gttctattgg aagcgtcaga ccgtctaggt        120

gggaacatta ctacgcaagg ggcagagggg ttcgtgtggg aactaggccc taacagcttc        180

gctccgactc ccgcgctttt acagcttata gcagaagtag ggcttcactc cgagcttata        240

cgtggcgatc gacatctacc gcgatacatt tattggcgag gcgaacttta tccattggag        300

ccgaccaggc cgcttgcttt ggcaacaagc aatctcctct ctccatgggg taaggtcaga        360

gcagcgttgg gcgccctcgg gtttgtcccc ccgtatctcg gatctggcga tgaatccgtt        420

aattcatttt tccgacgaca cctaggtcag gaggtagccg aacgtctggt ggcacctttc        480

gtctctggcg tgtatgcggg agatccccag caactgtccg ccgccgctgc atttagacga        540

atagcgcagc tggaaaaact aggtggggga ctcatagcag gagcattgag gctgaggaga        600

cagcaacccc ccaaacccag gcccccggca gaggttcaaa tgcgacccgg tgagttgggg        660

tctttcaaag agggattggc cgccttgcca cgtgcaatag ctcaacagct gaaagcacca        720

gtgcacctac aaaccccagt cgaagcaatc acgccggagc ccaacggagg ttatctactc        780

agatccggcg aacagacttg gcaagcgaga agcgtcgtcc tagcaacacc tgcctatcag        840

accgcagcat tagtggcgcc gtttcaacct gcaatagcgc gagtgttagc ggccattccg        900

tatccaacag tggcctgtgt agtactcgcc tatcccgcgg gactaggcag gtcagtacga        960

ccaggctttg gcgtccttat accccgtggc caaggtatcc gtacactcgg cactatatgg       1020

tctagctgct tatttccaca gcgaactcca gctgggtggc aagtttttac ttcattcatc       1080

ggtggagcaa cagatccgga cctcgcctca ctgagggaag aagcgatcgt tcaacaggtg       1140

caacaggact tgacccgtct acttgatctt cctgccgcca aggcgagact cctcggtatg       1200

aaagtgtggc gaagggcgat cccacaatac ttggtagggt atccacagca atggcagcaa       1260

gtaacccatg cccttagtca gacgccgggc cttttcctgt gtagtaacta cgccgagggc       1320

gtggcattag gcgacagggt tgaacatggt aacaggaccg ctgcggcagt ggccgcgtac       1380

ctgagtgggg ggcaatcctg a                                                 1401


<210>    9
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO16_XbaIF primer)


<400>    9
cccctctaga atgctagact ccctgattgt                                          30


<210>    10
<211>    31
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO16_XhoIR primer)


<400>    10
ccccctcgag ctccctgctt ctaatttttt g                                        31


<210>    11
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO17_XbaIF primer)


<400>    11
cccctctaga atggaggtcg atgttgcaat                                          30


<210>    12
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO17_XhoIR primer)


<400>    12
ccccctcgag ggattgcccc ccactcaggt                                          30


<210>    13
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360M forward primer)


<400>    13
catctgctga ccaatatgat cggcggcgca acg                                      33


<210>    14
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360M reverse primer)


<400>    14
cgttgcgccg ccgatcatat tggtcagcag atg                                      33


<210>    15
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360L forward primer)


<400>    15
gaccaatttg atcggcggcg caacggaccc tg                                       32


<210>    16
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360L reverse primer)


<400>    16
cgccgatcaa attggtcagc agatgctcac cc                                       32


<210>    17
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360I forward primer)


<400>    17
catctgctga ccaatatcat cggcggcgca acg                                      33


<210>    18
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360I reverse primer)


<400>    18
cgttgcgccg ccgatgatat tggtcagcag atg                                      33


<210>    19
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360C forward primer)


<400>    19
tgaccaattg catcggcggc gcaacggacc ctg                                      33


<210>    20
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360C reverse primer)


<400>    20
cgccgatgca attggtcagc agatgctcac cc                                       32


<210>    21
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360V forward primer)


<400>    21
ctgaccaatg tcatcggcgg cgcaacggac cc                                       32


<210>    22
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360V reverse primer)


<400>    22
gccgatgaca ttggtcagca gatgctcacc ctc                                      33


<210>    23
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360T forward primer)


<400>    23
catctgctga ccaataccat cggcggcgca acg                                      33


<210>    24
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F360T reverse primer)


<400>    24
cgttgcgccg ccgatggtat tggtcagcag atg                                      33


<210>    25
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V305L forward primer)


<400>    25
tatcctccgc tagcctgcgt agtcctagca tac                                      33


<210>    26
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V305L reverse primer)


<400>    26
cgcaggctag cggaggatag taaatttcct tg                                       32


<210>    27
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A162L forward primer)


<400>    27
gtctccggtg tgtatcttgg cgacgttgat caa                                      33


<210>    28
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A162L reverse primer)


<400>    28
ttgatcaacg tcgccaagat acacaccgga gac                                      33


<210>    29
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A162C forward primer)


<400>    29
gtctccggtg tgtattgtgg cgacgttgat caa                                      33


<210>    30
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A162C reverse primer)


<400>    30
ttgatcaacg tcgccacaat acacaccgga gac                                      33


<210>    31
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V305M forward primer)


<400>    31
atttactatc ctccgatggc ctgcgtagtc cta                                      33


<210>    32
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V305M reverse primer)


<400>    32
taggactacg caggccatcg gaggatagta aat                                      33


<210>    33
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (R85A forward primer)


<400>    33
gacagacgtc taccggcgtt tgtgtattgg aac                                      33


<210>    34
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (R85A reverse primer)


<400>    34
gttccaatac acaaacgccg gtagacgtct gtc                                      33


<210>    35
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F156A forward primer)


<400>    35
cgtttagtcg caccagcggt ctccggtgtg tatg                                     34


<210>    36
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F156A reverse primer)


<400>    36
catacacacc ggagaccgct ggtgcgacta aacg                                     34


<210>    37
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V160C forward primer)


<400>    37
ccatttgtct ccggttgcta tgctggcgac gttg                                     34


<210>    38
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V160C reverse primer)


<400>    38
caacgtcgcc agcatagcaa ccggagacaa atgg                                     34


<210>    39
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F324V forward primer)


<400>    39
cgtccattgg aaggtgtggg tcatcttata ccc                                      33


<210>    40
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F324V reverse primer)


<400>    40
gggtataaga tgacccacac cttccaatgg acg                                      33


<210>    41
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (L327T forward primer)


<400>    41
gaaggttttg gtcataccat acccaggaat cag                                      33


<210>    42
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (L327T reverse primer)


<400>    42
ctgattcctg ggtatggtat gaccaaaacc ttc                                      33


<210>    43
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (I340T forward primer)


<400>    43
aggactcttg gtacaacctg gtcctcctgt ctc                                      33


<210>    44
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (I340T reverse primer)


<400>    44
gagacaggag gaccaggttg taccaagagt cct                                      33


<210>    45
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V160C+A162C forward primer)


<400>    45
tgtctccggt tgctattgtg gcgacgttga tcaac                                    35


<210>    46
<211>    35
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V160C+A162C reverse primer)


<400>    46
gttgatcaac gtcgccacaa tagcaaccgg agaca                                    35


<210>    47
<211>    48
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V160C+A162L forward primer)


<400>    47
cgcaccattt gtctccggtt gctatcttgg cgacgttgat caactatc                      48


<210>    48
<211>    48
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V160C+A162L reverse primer)


<400>    48
gatagttgat caacgtcgcc aagatagcaa ccggagacaa atggtgcg                      48


<210>    49
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359M forward primer)


<400>    49
caagttttta cttcaatgat cggtggagca aca                                      33


<210>    50
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359M reverse primer)


<400>    50
tgttgctcca ccgatcattg aagtaaaaac ttg                                      33


<210>    51
<211>    32
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359C forward primer)


<400>    51
ccaccgatgc atgaagtaaa aacttgccac cc                                       32


<210>    52
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359C reverse primer)


<400>    52
tttacttcat gcatcggtgg agcaacagat ccg                                      33


<210>    53
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359L forward primer)


<400>    53
tccaccgatc aatgaagtaa aaacttgcca ccc                                      33


<210>    54
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359L reverse primer)


<400>    54
ttacttcatt gatcggtgga gcaacagatc cgg                                      33


<210>    55
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359I forward primer)


<400>    55
caagttttta cttcaatcat cggtggagca aca                                      33


<210>    56
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359I reverse primer)


<400>    56
tgttgctcca ccgatgattg aagtaaaaac ttg                                      33


<210>    57
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359V forward primer)


<400>    57
caagttttta cttcagtcat cggtggagca aca                                      33


<210>    58
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359V reverse primer)


<400>    58
tgttgctcca ccgatgactg aagtaaaaac ttg                                      33


<210>    59
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359T forward primer)


<400>    59
caagttttta cttcaaccat cggtggagca acag                                     34


<210>    60
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F359T reverse primer)


<400>    60
ctgttgctcc accgatggtt gaagtaaaaa cttg                                     34


<210>    61
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V304L forward primer)


<400>    61
tatccaacac tggcctgtgt agtactcgcc                                          30


<210>    62
<211>    31
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V304L reverse primer)


<400>    62
cacaggccag tgttggatac ggaatggccg c                                        31


<210>    63
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A166L forward primer)


<400>    63
gtctctggcg tgtatctggg agatccccag caa                                      33


<210>    64
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A166L reverse primer)


<400>    64
ttgctgggga tctcccagat acacgccaga gac                                      33


<210>    65
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A166C forward primer)


<400>    65
gtctctggcg tgtattgcgg agatccccag caa                                      33


<210>    66
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (A166C reverse primer)


<400>    66
ttgctgggga tctccgcaat acacgccaga gac                                      33


<210>    67
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V304M forward primer)


<400>    67
attccgtatc caacaatggc ctgtgtagta ctc                                      33


<210>    68
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V304M reverse primer)


<400>    68
gagtactaca caggccattg ttggatacgg aat                                      33


<210>    69
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (R88A forward primer)


<400>    69
gatcgacatc taccggcgta catttattgg cgag                                     34


<210>    70
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (R88A reverse primer)


<400>    70
ctcgccaata aatgtacgcc ggtagatgtc gatc                                     34


<210>    71
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F160A forward primer)


<400>    71
cgtctggtgg cacctgcggt ctctggcgtg tatg                                     34


<210>    72
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F160A reverse primer)


<400>    72
catacacgcc agagaccgca ggtgccacca gacg                                     34


<210>    73
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V164C forward primer)


<400>    73
cctttcgtct ctggctgcta tgcgggagat ccc                                      33


<210>    74
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V164C reverse primer)


<400>    74
gggatctccc gcatagcagc cagagacgaa agg                                      33


<210>    75
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F323V forward primer)


<400>    75
gtcagtacga ccaggcgtgg gcgtccttat accc                                     34


<210>    76
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (F323V reverse primer)


<400>    76
gggtataagg acgcccacgc ctggtcgtac tgac                                     34


<210>    77
<211>    42
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (L326T forward primer)


<400>    77
ggctttggcg tcactatacc ccgtggccaa ggtatccgta ca                            42


<210>    78
<211>    42
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (L326T reverse primer)


<400>    78
gccacggggt atagtgacgc caaagcctgg tcgtactgac ct                            42


<210>    79
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (I339T forward primer)


<400>    79
cgtacactcg gcactacctg gtctagctgc tta                                      33


<210>    80
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (I339T reverse primer)


<400>    80
taagcagcta gaccaggtag tgccgagtgt acg                                      33


<210>    81
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V164C+A166L forward primer)


<400>    81
cctttcgtct ctggctgcta tctgggagat ccccagcaa                                39


<210>    82
<211>    39
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V164C+A166L reverse primer)


<400>    82
ttgctgggga tctcccagat agcagccaga gacgaaagg                                39


<210>    83
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V164C+A166C forward primer)


<400>    83
ttcgtctctg gctgctattg cggagatccc cag                                      33


<210>    84
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (V164C+A166C reverse primer)


<400>    84
ctggggatct ccgcaatagc agccagagac gaa                                      33


<210>    85
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO16_BamHIF primer)


<400>    85
ccccggatcc atgctagact ccctgattgt                                          30


<210>    86
<211>    34
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO16_SalIR primer)


<400>    86
ccccgtcgac tcactccctg cttctaattt tttg                                     34


<210>    87
<211>    30
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO17_BamHIF primer)


<400>    87
ccccggatcc atggaggtcg atgttgcaat                                          30


<210>    88
<211>    33
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (CyPPO17_SalIR primer)


<400>    88
ccccgtcgac tcaggattgc cccccactca ggt                                      33


<210>    89
<211>    1614
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Gene for AtPPO1 of Arabidopsis thaliana


<400>    89
atggagttat ctcttctccg tccgacgact caatcgcttc ttccgtcgtt ttcgaagccc         60

aatctccgat taaatgttta taagcctctt agactccgtt gttcagtggc cggtggacca        120

accgtcggat cttcaaaaat cgaaggcgga ggaggcacca ccatcacgac ggattgtgtg        180

attgtcggcg gaggtattag tggtctttgc atcgctcagg cgcttgctac taagcatcct        240

gatgctgctc cgaatttaat tgtgaccgag gctaaggatc gtgttggagg caacattatc        300

actcgtgaag agaatggttt tctctgggaa gaaggtccca atagttttca accgtctgat        360

cctatgctca ctatggtggt agatagtggt ttgaaggatg atttggtgtt gggagatcct        420

actgcgccaa ggtttgtgtt gtggaatggg aaattgaggc cggttccatc gaagctaaca        480

gacttaccgt tctttgattt gatgagtatt ggtgggaaga ttagagctgg ttttggtgca        540

cttggcattc gaccgtcacc tccaggtcgt gaagaatctg tggaggagtt tgtacggcgt        600

aacctcggtg atgaggtttt tgagcgcctg attgaaccgt tttgttcagg tgtttatgct        660

ggtgatcctt caaaactgag catgaaagca gcgtttggga aggtttggaa actagagcaa        720

aatggtggaa gcataatagg tggtactttt aaggcaattc aggagaggaa aaacgctccc        780

aaggcagaac gagacccgcg cctgccaaaa ccacagggcc aaacagttgg ttctttcagg        840

aagggacttc gaatgttgcc agaagcaata tctgcaagat taggtagcaa agttaagttg        900

tcttggaagc tctcaggtat cactaagctg gagagcggag gatacaactt aacatatgag        960

actccagatg gtttagtttc cgtgcagagc aaaagtgttg taatgacggt gccatctcat       1020

gttgcaagtg gtctcttgcg ccctctttct gaatctgctg caaatgcact ctcaaaacta       1080

tattacccac cagttgcagc agtatctatc tcgtacccga aagaagcaat ccgaacagaa       1140

tgtttgatag atggtgaact aaagggtttt gggcaattgc atccacgcac gcaaggagtt       1200

gaaacattag gaactatcta cagctcctca ctctttccaa atcgcgcacc gcccggaaga       1260

attttgctgt tgaactacat tggcgggtct acaaacaccg gaattctgtc caagtctgaa       1320

ggtgagttag tggaagcagt tgacagagat ttgaggaaaa tgctaattaa gcctaattcg       1380

accgatccac ttaaattagg agttagggta tggcctcaag ccattcctca gtttctagtt       1440

ggtcactttg atatccttga cacggctaaa tcatctctaa cgtcttcggg ctacgaaggg       1500

ctatttttgg gtggcaatta cgtcgctggt gtagccttag gccggtgtgt agaaggcgca       1560

tatgaaaccg cgattgaggt caacaacttc atgtcacggt acgcttacaa gtaa             1614


<210>    90
<211>    155
<212>    DNA
<213>    Artificial Sequence

<220>
<223>    Synthetic (Gene for Transit Peptide of AtPPO1)


<400>    90
atggagttat tcttctccgt ccgacgactc aatcgcttct tccgtcgttt tcgaagccca         60

atctccgatt aaatgtttat aagcctctta gactccgttg ttcagtggcc ggtggaccaa        120

ccgtcggatc ttcaaaaatc gaaggcggag gaggc                                   155
