                         SEQUENCE LISTING

<110>  Exxon Mobil Research and Engineering
       Summers, Zarath
       Volzing, Katherine
 
<120>  Biological Upgrading of Hydrocarbon Streams With 
       Nickel Binding Proteins

<130>  PM2017021

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  302
<212>  PRT
<213>  Bradyrhizobium japonicum

<400>  1

Met Cys Thr Val Cys Gly Cys Ser Asp Gly Lys Ala Ser Ile Glu His 
1               5                   10                  15      


Ala His Asp His His His Asp His Gly His Asp His Asp His Gly His 
            20                  25                  30          


Asp Gly His His His His His His Gly His Asp Gln Asp His His His 
        35                  40                  45              


His His Asp His Ala His Gly Asp Ala Gly Leu Leu Asp Cys Gly Ala 
    50                  55                  60                  


Asn Pro Ala Gly Gln Lys Ile Thr Gly Met Ser Ser Asp Arg Ile Ile 
65                  70                  75                  80  


Gln Val Glu Arg Asp Ile Leu Gly Lys Asn Asp Arg Leu Ala Ala Asp 
                85                  90                  95      


Asn Arg Ala Arg Phe Arg Ala Asp Glu Val Leu Ala Phe Asn Leu Val 
            100                 105                 110         


Ser Ser Pro Gly Ala Gly Lys Thr Ser Leu Leu Val Arg Ala Val Ser 
        115                 120                 125             


Glu Leu Lys Asp Ser Phe Ala Ile Gly Val Ile Glu Gly Asp Gln Gln 
    130                 135                 140                 


Thr Ser Asn Asp Ala Glu Arg Ile Arg Ala Thr Gly Val Pro Ala Ile 
145                 150                 155                 160 


Gln Val Asn Thr Gly Lys Gly Cys His Leu Asp Ala Ala Met Val Gly 
                165                 170                 175     


Glu Ala Tyr Asp Arg Leu Pro Trp Leu Asn Gly Gly Leu Leu Phe Ile 
            180                 185                 190         


Glu Asn Val Gly Asn Leu Val Cys Pro Ala Ala Phe Asp Leu Gly Glu 
        195                 200                 205             


Ala Cys Lys Ile Val Val Phe Ser Thr Thr Glu Gly Glu Asp Lys Pro 
    210                 215                 220                 


Leu Lys Tyr Pro Asp Met Phe Ala Ala Ser Ser Leu Met Leu Ile Asn 
225                 230                 235                 240 


Lys Ile Asp Leu Ala Ser Val Leu Asp Phe Asp Leu Ala Arg Thr Ile 
                245                 250                 255     


Glu Tyr Ala Arg Arg Val Asn Pro Lys Ile Glu Val Leu Thr Leu Ser 
            260                 265                 270         


Ala Arg Thr Gly Glu Gly Phe Ala Ala Phe Tyr Ala Trp Ile Arg Lys 
        275                 280                 285             


Arg Met Ala Ala Thr Thr Pro Ala Ala Met Thr Ala Ala Glu 
    290                 295                 300         


<210>  2
<211>  290
<212>  PRT
<213>  Escherichia coli

<400>  2

Met Cys Thr Thr Cys Gly Cys Gly Glu Gly Asn Leu Tyr Ile Glu Gly 
1               5                   10                  15      


Asp Glu His Asn Pro His Ser Ala Phe Arg Ser Ala Pro Phe Ala Pro 
            20                  25                  30          


Ala Ala Arg Pro Lys Met Lys Ile Thr Gly Ile Lys Ala Pro Glu Phe 
        35                  40                  45              


Thr Pro Ser Gln Thr Glu Glu Gly Asp Leu His Tyr Gly His Gly Glu 
    50                  55                  60                  


Ala Gly Thr His Ala Pro Gly Met Ser Gln Arg Arg Met Leu Glu Val 
65                  70                  75                  80  


Glu Ile Asp Val Leu Asp Lys Asn Asn Arg Leu Ala Glu Arg Asn Arg 
                85                  90                  95      


Ala Arg Phe Ala Ala Arg Lys Gln Leu Val Leu Asn Leu Val Ser Ser 
            100                 105                 110         


Pro Gly Ser Gly Lys Thr Thr Leu Leu Thr Glu Thr Leu Met Arg Leu 
        115                 120                 125             


Lys Asp Ser Val Pro Cys Ala Val Ile Glu Gly Asp Gln Gln Thr Val 
    130                 135                 140                 


Asn Asp Ala Ala Arg Ile Arg Ala Thr Gly Thr Pro Ala Ile Gln Val 
145                 150                 155                 160 


Asn Thr Gly Lys Gly Cys His Leu Asp Ala Gln Met Ile Ala Asp Ala 
                165                 170                 175     


Ala Pro Arg Leu Pro Leu Asp Asp Asn Gly Ile Leu Phe Ile Glu Asn 
            180                 185                 190         


Val Gly Asn Leu Val Cys Pro Ala Ser Phe Asp Leu Gly Glu Lys His 
        195                 200                 205             


Lys Val Ala Val Leu Ser Val Thr Glu Gly Glu Asp Lys Pro Leu Lys 
    210                 215                 220                 


Tyr Pro His Met Phe Ala Ala Ala Ser Leu Met Leu Leu Asn Lys Val 
225                 230                 235                 240 


Asp Leu Leu Pro Tyr Leu Asn Phe Asp Val Glu Lys Cys Ile Ala Cys 
                245                 250                 255     


Ala Arg Glu Val Asn Pro Glu Ile Glu Ile Ile Leu Ile Ser Ala Thr 
            260                 265                 270         


Ser Gly Glu Gly Met Asp Gln Trp Leu Asn Trp Leu Glu Thr Gln Arg 
        275                 280                 285             


Cys Ala 
    290 


<210>  3
<211>  117
<212>  PRT
<213>  Helicobacter pylori

<400>  3

Met His Glu Tyr Ser Val Val Ser Ser Leu Ile Ala Leu Cys Glu Glu 
1               5                   10                  15      


His Ala Lys Lys Asn Gln Ala His Lys Ile Glu Arg Val Val Val Gly 
            20                  25                  30          


Ile Gly Glu Arg Ser Ala Met Asp Lys Ser Leu Phe Val Ser Ala Phe 
        35                  40                  45              


Glu Thr Phe Arg Glu Glu Ser Leu Val Cys Lys Asp Ala Val Leu Asp 
    50                  55                  60                  


Ile Val Asp Glu Lys Val Glu Leu Glu Cys Lys Asp Cys Ser His Val 
65                  70                  75                  80  


Phe Lys Pro Asn Ala Leu Asp Tyr Gly Val Cys Glu Lys Cys His Ser 
                85                  90                  95      


Lys Asn Val Val Ile Thr Gln Gly Asn Glu Met Arg Leu Leu Ser Leu 
            100                 105                 110         


Glu Met Leu Ala Glu 
        115         


<210>  4
<211>  196
<212>  PRT
<213>  Escherichia coli

<400>  4

Met Lys Val Ala Lys Asp Leu Val Val Ser Leu Ala Tyr Gln Val Arg 
1               5                   10                  15      


Thr Glu Asp Gly Val Leu Val Asp Glu Ser Pro Val Ser Ala Pro Leu 
            20                  25                  30          


Asp Tyr Leu His Gly His Gly Ser Leu Ile Ser Gly Leu Glu Thr Ala 
        35                  40                  45              


Leu Glu Gly His Glu Val Gly Asp Lys Phe Asp Val Ala Val Gly Ala 
    50                  55                  60                  


Asn Asp Ala Tyr Gly Gln Tyr Asp Glu Asn Leu Val Gln Arg Val Pro 
65                  70                  75                  80  


Lys Asp Val Phe Met Gly Val Asp Glu Leu Gln Val Gly Met Arg Phe 
                85                  90                  95      


Leu Ala Glu Thr Asp Gln Gly Pro Val Pro Val Glu Ile Thr Ala Val 
            100                 105                 110         


Glu Asp Asp His Val Val Val Asp Gly Asn His Met Leu Ala Gly Gln 
        115                 120                 125             


Asn Leu Lys Phe Asn Val Glu Val Val Ala Ile Arg Glu Ala Thr Glu 
    130                 135                 140                 


Glu Glu Leu Ala His Gly His Val His Gly Ala His Asp His His His 
145                 150                 155                 160 


Asp His Asp His Asp Gly Cys Cys Gly Gly His Gly His Asp His Gly 
                165                 170                 175     


His Glu His Gly Gly Glu Gly Cys Cys Gly Gly Lys Gly Asn Gly Gly 
            180                 185                 190         


Cys Gly Cys His 
        195     


<210>  5
<211>  158
<212>  PRT
<213>  Enterobacter aerogenes

<400>  5

Met Leu Tyr Leu Thr Gln Arg Leu Glu Ile Pro Ala Ala Ala Thr Ala 
1               5                   10                  15      


Ser Val Thr Leu Pro Ile Asp Val Arg Val Lys Ser Arg Val Lys Val 
            20                  25                  30          


Thr Leu Asn Asp Gly Arg Asp Ala Gly Leu Leu Leu Pro Arg Gly Leu 
        35                  40                  45              


Leu Leu Arg Gly Gly Asp Val Leu Ser Asn Glu Glu Gly Thr Glu Phe 
    50                  55                  60                  


Val Gln Val Ile Ala Ala Asp Glu Glu Val Ser Val Val Arg Cys Asp 
65                  70                  75                  80  


Asp Pro Phe Met Leu Ala Lys Ala Cys Tyr His Leu Gly Asn Arg His 
                85                  90                  95      


Val Pro Leu Gln Ile Met Pro Gly Glu Leu Arg Tyr His His Asp His 
            100                 105                 110         


Val Leu Asp Asp Met Leu Arg Gln Phe Gly Leu Thr Val Thr Phe Gly 
        115                 120                 125             


Gln Leu Pro Phe Glu Pro Glu Ala Gly Ala Tyr Ala Ser Glu Ser His 
    130                 135                 140                 


Gly His His His Ala His His Asp His His Ala His Ser His 
145                 150                 155             


<210>  6
<211>  148
<212>  PRT
<213>  Helicobacter pylori

<400>  6

Met Asp Thr Pro Asn Lys Asp Asp Ser Ile Ile Arg Phe Ser Val Ser 
1               5                   10                  15      


Leu Gln Gln Asn Leu Leu Asp Glu Leu Asp Asn Arg Ile Ile Lys Asn 
            20                  25                  30          


Gly Tyr Ser Ser Arg Ser Glu Leu Val Arg Asp Met Ile Arg Glu Lys 
        35                  40                  45              


Leu Val Glu Asp Asn Trp Ala Glu Asp Asn Pro Asn Asp Gly Ser Lys 
    50                  55                  60                  


Ile Ala Val Leu Val Val Ile Tyr Asp His His Gln Arg Glu Leu Asn 
65                  70                  75                  80  


Gln Arg Met Ile Asp Ile Gln His Thr Ser Gly Thr His Val Leu Cys 
                85                  90                  95      


Thr Thr His Ile His Met Asp Gly His Asn Cys Leu Glu Thr Ile Ile 
            100                 105                 110         


Leu Lys Gly Asn Ser Leu Glu Ile Gln Arg Leu Gln Leu Glu Ile Gly 
        115                 120                 125             


Gly Leu Arg Gly Val Lys Phe Ala Lys Leu Thr Lys Ala Ser Ser Phe 
    130                 135                 140                 


Glu His Asn Glu 
145             


<210>  7
<211>  135
<212>  PRT
<213>  Escherichia coli

<400>  7

Met Arg Leu Leu His Thr Met Leu Arg Val Gly Asp Leu Gln Arg Ser 
1               5                   10                  15      


Ile Asp Phe Tyr Thr Lys Val Leu Gly Met Lys Leu Leu Arg Thr Ser 
            20                  25                  30          


Glu Asn Pro Glu Tyr Lys Tyr Ser Leu Ala Phe Val Gly Tyr Gly Pro 
        35                  40                  45              


Glu Thr Glu Glu Ala Val Ile Glu Leu Thr Tyr Asn Trp Gly Val Asp 
    50                  55                  60                  


Lys Tyr Glu Leu Gly Thr Ala Tyr Gly His Ile Ala Leu Ser Val Asp 
65                  70                  75                  80  


Asn Ala Ala Glu Ala Cys Glu Lys Ile Arg Gln Asn Gly Gly Asn Val 
                85                  90                  95      


Thr Arg Glu Ala Gly Pro Val Lys Gly Gly Thr Thr Val Ile Ala Phe 
            100                 105                 110         


Val Glu Asp Pro Asp Gly Tyr Lys Ile Glu Leu Ile Glu Glu Lys Asp 
        115                 120                 125             


Ala Gly Arg Gly Leu Gly Asn 
    130                 135 


<210>  8
<211>  60
<212>  PRT
<213>  Helicobacter pylori

<400>  8

Met Ala His His Glu Glu Gln His Gly Gly His His His His His His 
1               5                   10                  15      


His Thr His His His His Tyr His Gly Gly Glu His His His His His 
            20                  25                  30          


His Ser Ser His His Glu Glu Gly Cys Cys Ser Thr Ser Asp Ser His 
        35                  40                  45              


His Gln Glu Glu Gly Cys Cys His Gly His His Glu 
    50                  55                  60  


<210>  9
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer to T7 promoter

<400>  9
taatacgact cactataggg                                                   20


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer to terminator

<400>  10
gctagttatt gctcagcgg                                                    19


<210>  11
<211>  5
<212>  PRT
<213>  Helicobacter pylori

<400>  11

Glu Glu Gly Cys Cys 
1               5   


<210>  12
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Consensus motif for nickel binding site


<220>
<221>  Xaa
<222>  (2)..(3)
<223>  Can be any amino acid

<400>  12

Cys Xaa Xaa Cys Gly Cys 
1               5       

