                         SEQUENCE LISTING

<110>  Exxon Mobil Research and Engineering
       Summers, Zarath
       Patel, Jay
       Marler, David
 
<120>  BIOLOGICAL UPGRADING OF HYDROCARBON STREAMS WITH OXYGENASES

<130>  PM2017032

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  224
<212>  PRT
<213>  Corynebacterium jeikeium

<400>  1

Met Ala Ser Thr Ser Thr Leu Ser Asn Thr Ser Phe Asn Thr Ala Glu 
1               5                   10                  15      


Pro Ile Ser Gln Ala Leu Arg Asn Ala Thr Ala Glu Ala His Gly Arg 
            20                  25                  30          


Ala Glu Gly Ser Glu Phe Met Ser Thr Leu Phe Lys Gly Glu Leu Asp 
        35                  40                  45              


Ala Gln Ala Val Tyr Ala Leu Ser Gly Gln Leu Trp Phe Val Tyr Ser 
    50                  55                  60                  


Ala Leu Glu Asn Ala Val Glu Arg Val Ser Gly Thr Pro Ile Gly Ser 
65                  70                  75                  80  


Val Ile Ala Asp Pro Arg Leu Gln Arg Cys Ala Ala Leu Glu His Asp 
                85                  90                  95      


Leu Thr Tyr Cys Leu Gly Ser Asp Trp Arg Glu Gln Leu Glu Leu Leu 
            100                 105                 110         


Pro Ala Thr Arg Arg Tyr Val Asp Arg Leu Asp Ser Phe Gly Glu Gln 
        115                 120                 125             


Asp Val Val Arg Val Ile Ala His His Tyr Val Arg Tyr Leu Gly Asp 
    130                 135                 140                 


Ile Ser Gly Gly Gln Val Ile Ala Val Arg Val Ala Asp Leu Tyr Asn 
145                 150                 155                 160 


Val Ala Pro Glu Ala Leu Lys Phe Tyr Asp Phe Ser Ala Ile Gly Lys 
                165                 170                 175     


Ile Pro Pro Tyr Arg Thr Ser Tyr Arg Gln Arg Leu Asp Ser Leu Pro 
            180                 185                 190         


Leu Thr Ala Gln Gln Arg Ser Glu Leu Ile Glu Glu Ala Ile Asp Ala 
        195                 200                 205             


Phe Gly Met Asn Phe Ser Leu Phe Thr Asp Leu Tyr Gly Val Cys Ala 
    210                 215                 220                 


<210>  2
<211>  198
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  2

Met Asp Thr Leu Ala Pro Glu Ser Thr Arg Gln Asn Leu Arg Ser Gln 
1               5                   10                  15      


Arg Leu Asn Leu Leu Thr Asn Glu Pro His Gln Arg Leu Glu Ser Leu 
            20                  25                  30          


Val Lys Ser Lys Glu Pro Phe Ala Ser Arg Asp Asn Phe Ala Arg Phe 
        35                  40                  45              


Val Ala Ala Gln Tyr Leu Phe Gln His Asp Leu Glu Pro Leu Tyr Arg 
    50                  55                  60                  


Asn Glu Ala Leu Ala Arg Leu Phe Pro Gly Leu Ala Ser Arg Ala Arg 
65                  70                  75                  80  


Asp Asp Ala Ala Arg Ala Asp Leu Ala Asp Leu Gly His Pro Val Pro 
                85                  90                  95      


Glu Gly Asp Gln Ser Val Arg Glu Ala Asp Leu Ser Leu Ala Glu Ala 
            100                 105                 110         


Leu Gly Trp Leu Phe Val Ser Glu Gly Ser Lys Leu Gly Ala Ala Phe 
        115                 120                 125             


Leu Phe Lys Lys Ala Ala Ala Leu Glu Leu Asp Glu Asn Phe Gly Ala 
    130                 135                 140                 


Arg His Leu Ala Glu Pro Glu Gly Gly Arg Ala Gln Gly Trp Lys Ser 
145                 150                 155                 160 


Phe Val Ala Ile Leu Asp Gly Ile Glu Leu Asn Glu Glu Glu Glu Arg 
                165                 170                 175     


Leu Ala Ala Lys Gly Ala Ser Asp Ala Phe Asn Arg Phe Gly Asp Leu 
            180                 185                 190         


Leu Glu Arg Thr Phe Ala 
        195             


<210>  3
<211>  342
<212>  PRT
<213>  Escherichia coli

<400>  3

Met Asn His Tyr Thr Arg Trp Leu Glu Leu Lys Glu Gln Asn Pro Gly 
1               5                   10                  15      


Lys Tyr Ala Arg Asp Ile Ala Gly Leu Met Asn Ile Ser Glu Ala Glu 
            20                  25                  30          


Leu Ala Phe Ala Arg Val Thr His Asp Ala Trp Arg Met Arg Gly Asp 
        35                  40                  45              


Ile Arg Glu Ile Leu Ala Ala Leu Glu Ser Val Gly Glu Thr Lys Cys 
    50                  55                  60                  


Ile Cys Arg Asn Glu Tyr Ala Val His Glu Gln Val Gly Ala Phe Thr 
65                  70                  75                  80  


Asn Gln His Leu Asn Gly His Ala Gly Leu Ile Leu Asn Pro Arg Ala 
                85                  90                  95      


Leu Asp Leu Arg Leu Phe Leu Asn Gln Trp Ala Ser Val Phe His Ile 
            100                 105                 110         


Lys Glu Asn Thr Ala Arg Gly Glu Arg Gln Ser Ile Gln Phe Phe Asp 
        115                 120                 125             


His Gln Gly Asp Ala Leu Leu Lys Val Tyr Ala Thr Asp Asn Thr Asp 
    130                 135                 140                 


Met Ala Ala Trp Ser Glu Leu Leu Ala Arg Phe Ile Thr Asp Glu Asn 
145                 150                 155                 160 


Thr Pro Leu Glu Leu Lys Ala Val Asp Ala Pro Val Val Gln Thr Arg 
                165                 170                 175     


Ala Asp Ala Ser Val Val Glu Gln Glu Trp Arg Ala Met Thr Asp Val 
            180                 185                 190         


His Gln Phe Phe Thr Leu Leu Lys Arg His Asn Leu Thr Arg Gln Gln 
        195                 200                 205             


Ala Phe Asn Leu Val Ala Asp Asp Leu Ala Cys Lys Val Ser Asn Ser 
    210                 215                 220                 


Ala Leu Ala Gln Ile Leu Glu Ser Ala Gln Gln Asp Gly Asn Glu Ile 
225                 230                 235                 240 


Met Val Phe Val Gly Asn Arg Gly Cys Val Gln Ile Phe Thr Gly Val 
                245                 250                 255     


Val Glu Lys Val Val Pro Met Lys Gly Trp Leu Asn Ile Phe Asn Pro 
            260                 265                 270         


Thr Phe Thr Leu His Leu Leu Glu Glu Ser Ile Ala Glu Thr Trp Val 
        275                 280                 285             


Thr Arg Lys Pro Ala Ser Asp Gly Tyr Val Thr Ser Leu Glu Leu Phe 
    290                 295                 300                 


Ala His Asp Gly Thr Gln Ile Ala Gln Leu Tyr Gly Gln Arg Thr Glu 
305                 310                 315                 320 


Gly Glu Gln Glu Gln Ala Gln Trp Arg Lys Gln Ile Ala Ser Leu Ile 
                325                 330                 335     


Pro Glu Gly Val Thr Ala 
            340         


<210>  4
<211>  360
<212>  PRT
<213>  Pseudomonas aeruginosa

<400>  4

His His His His His His Met Ser Ser Thr Pro Ser Leu Ser His Ser 
1               5                   10                  15      


Pro Ala Glu Leu Tyr Arg Ala Trp Gln Asp Leu Arg Ala Glu Arg Pro 
            20                  25                  30          


Gln Leu Arg Ala Arg Asp Ala Ala Ala Leu Leu Gln Val Ser Glu Gly 
        35                  40                  45              


Glu Leu Val Ala Ser Arg Val Gly Ile Asp Ala Val Arg Leu Arg Pro 
    50                  55                  60                  


Asp Trp Ala Ala Leu Leu Pro Ala Leu Gly Glu Leu Gly Pro Ile Met 
65                  70                  75                  80  


Ala Leu Thr Arg Asn Glu His Cys Val His Glu Arg Lys Gly Pro Tyr 
                85                  90                  95      


Arg Glu Val Thr Val Ser Ala Asn Gly Gln Met Gly Leu Val Val Ser 
            100                 105                 110         


Pro Asp Ile Asp Leu Arg Leu Phe Leu Gly Gly Trp Asn Ala Val Phe 
        115                 120                 125             


Ala Ile Ala Glu Glu Thr Ala Arg Gly Thr Gln Arg Ser Ile Gln Val 
    130                 135                 140                 


Phe Asp Gln Gln Gly Val Ala Val His Lys Val Phe Leu Ala Glu Ala 
145                 150                 155                 160 


Ser Asp Val Arg Ala Trp Glu Pro Leu Val Glu Arg Leu Arg Ala Ala 
                165                 170                 175     


Glu Gln Asp Ala Val Leu Ala Leu His Glu Pro Arg Ala Pro Ala Ala 
            180                 185                 190         


Ala Leu Val Asp Ala Gln Ile Asp Ala Ala Ala Leu Arg Glu Gly Trp 
        195                 200                 205             


Ala Ala Leu Lys Asp Thr His His Phe His Ala Leu Leu Lys Lys His 
    210                 215                 220                 


Gly Ala Gln Arg Thr Gln Ala Leu Arg Leu Ala Gly Gly Glu Trp Ala 
225                 230                 235                 240 


Glu Arg Leu Asp Asn Gly Asp Leu Ala Lys Leu Phe Glu Ala Ala Ala 
                245                 250                 255     


Glu Ser Gly Leu Pro Ile Met Val Phe Val Gly Asn Ala His Cys Ile 
            260                 265                 270         


Gln Ile His Thr Gly Pro Val Cys Asn Leu Lys Trp Leu Asp Asp Trp 
        275                 280                 285             


Phe Asn Val Leu Asp Pro Glu Phe Asn Leu His Leu Lys Thr Thr Gly 
    290                 295                 300                 


Ile Ala Glu Leu Trp Arg Val Arg Lys Pro Ser Thr Asp Gly Ile Val 
305                 310                 315                 320 


Thr Ser Trp Glu Ala Phe Asp Pro Asp Gly Glu Leu Ile Val Gln Leu 
                325                 330                 335     


Phe Gly Ala Arg Lys Pro Gly Glu Pro Glu Arg Asp Asp Trp Arg Glu 
            340                 345                 350         


Leu Ala Glu Ser Phe Lys Ala Leu 
        355                 360 


<210>  5
<211>  251
<212>  PRT
<213>  Helicobacter pylori

<400>  5

Met Leu Asn Arg Ile Ile Glu His Met Asn Ala His His Val Glu Asp 
1               5                   10                  15      


Met Lys Gly Leu Leu Lys Lys Phe Gly Gln Val His His Ala Glu Asn 
            20                  25                  30          


Val Ala Phe Lys Ser Val Asp Ser Gln Gly Val Val Ile Gly Tyr Asn 
        35                  40                  45              


Asn Asn Gln Thr Leu Arg Ile Glu Phe Asn His Glu Val Lys Asp Pro 
    50                  55                  60                  


Lys Asp Tyr Lys Asn Ala Ile Ile Glu Leu Cys Gln Ser Val Glu Lys 
65                  70                  75                  80  


Thr His Asp Leu Lys Gly Val Glu Glu Glu Val Lys Ala Phe Lys Glu 
                85                  90                  95      


Gly Phe Asp Ser Val Cys Leu Ala Thr Leu His Pro Asn Gly His Val 
            100                 105                 110         


Val Cys Ser Tyr Ala Pro Leu Met Ser Asp Gly Lys Gln Tyr Tyr Ile 
        115                 120                 125             


Tyr Val Ser Glu Val Ala Glu His Phe Ala Gly Leu Lys His Asn Pro 
    130                 135                 140                 


His Asn Val Glu Val Met Phe Leu Glu Asp Glu Ser Lys Ala Lys Ser 
145                 150                 155                 160 


Ala Ile Leu Arg Lys Arg Leu Arg Tyr Lys Thr Asn Ala Arg Phe Ile 
                165                 170                 175     


Glu Arg Gly Ala Glu Phe Asp Lys Ala Phe Asp Ser Phe Ile Glu Lys 
            180                 185                 190         


Thr Gly Gly Ala Gly Gly Ile Lys Thr Ile Arg Thr Met Gln Asp Phe 
        195                 200                 205             


His Leu Ile Ala Leu Asp Phe Lys Glu Gly Arg Phe Val Lys Gly Phe 
    210                 215                 220                 


Gly Gln Ala Tyr Asp Ile Leu Gly Asp Lys Ile Ala Tyr Val Gly Asp 
225                 230                 235                 240 


Lys Gly Asn Pro His Asp Phe Ala His Lys Lys 
                245                 250     


<210>  6
<211>  108
<212>  PRT
<213>  Staphylococcus aureus

<400>  6

Met Phe Met Ala Glu Asn Arg Leu Gln Leu Gln Lys Gly Ser Ala Glu 
1               5                   10                  15      


Glu Thr Ile Glu Arg Phe Tyr Asn Arg Gln Gly Ile Glu Thr Ile Glu 
            20                  25                  30          


Gly Phe Gln Gln Met Phe Val Thr Lys Thr Leu Asn Thr Glu Asp Thr 
        35                  40                  45              


Asp Glu Val Lys Ile Leu Thr Ile Trp Glu Ser Glu Asp Ser Phe Asn 
    50                  55                  60                  


Asn Trp Leu Asn Ser Asp Val Phe Lys Glu Ala His Lys Asn Val Arg 
65                  70                  75                  80  


Leu Lys Ser Asp Asp Asp Gly Gln Gln Ser Pro Ile Leu Ser Asn Lys 
                85                  90                  95      


Val Phe Lys Tyr Asp Ile Gly Tyr His Tyr Gln Lys 
            100                 105             


<210>  7
<211>  123
<212>  PRT
<213>  Mycobacterium tuberculosis

<400>  7

Met Pro Val Val Lys Ile Asn Ala Ile Glu Val Pro Ala Gly Ala Gly 
1               5                   10                  15      


Pro Glu Leu Glu Lys Arg Phe Ala His Arg Ala His Ala Val Glu Asn 
            20                  25                  30          


Ser Pro Gly Phe Leu Gly Phe Gln Leu Leu Arg Pro Val Lys Gly Glu 
        35                  40                  45              


Glu Arg Tyr Phe Val Val Thr His Trp Glu Ser Asp Glu Ala Phe Gln 
    50                  55                  60                  


Ala Trp Ala Asn Gly Pro Ala Ile Ala Ala His Ala Gly His Arg Ala 
65                  70                  75                  80  


Asn Pro Val Ala Thr Gly Ala Ser Leu Leu Glu Phe Glu Val Val Leu 
                85                  90                  95      


Asp Val Gly Gly Thr Gly Lys Thr Ala Gly Val Pro Arg Gly Lys Leu 
            100                 105                 110         


Ala Ala Ala Leu Glu His His His His His His 
        115                 120             


<210>  8
<211>  165
<212>  PRT
<213>  Bacillus thuringiensis

<400>  8

Met Lys Ala Ile Ile Ser Tyr Glu Thr Pro Leu Glu Gln Ser His Phe 
1               5                   10                  15      


Thr Ala Lys Asn Asp Glu Lys Asn Met Phe Tyr Lys Glu Asn Thr Glu 
            20                  25                  30          


Glu Ser Val Glu Gly Ser Leu Gln Tyr Asp Val Leu Asp Ala Val Gly 
        35                  40                  45              


Glu Phe Lys Gly Gln Ser Gly Tyr Ile Val Cys Asn Asn Ile Ser Val 
    50                  55                  60                  


Thr Asp Glu Gly Arg Pro Val Phe Glu Asn Arg Phe Lys Asn Arg Ala 
65                  70                  75                  80  


Gly Leu Ile Glu Asn Glu Pro Gly Phe Gln Ala Ile Arg Val Leu Arg 
                85                  90                  95      


Pro Leu Ser Asn Asp Thr Tyr Val Ile Leu Thr Met Trp Glu Thr Glu 
            100                 105                 110         


Gln Asn Phe Lys Asp Trp Thr Glu Ser Arg Ser Phe Glu Asn Ala His 
        115                 120                 125             


Lys Lys Arg Pro Thr Gln Ala Glu Gly Gln Ala Pro Ala His Pro His 
    130                 135                 140                 


Ala Glu Gln Gln Lys Ser Ile Phe Ser Arg Pro Ser Phe Val Thr Thr 
145                 150                 155                 160 


Phe Asp Val Leu Val 
                165 


<210>  9
<211>  107
<212>  PRT
<213>  Staphylococcus aureus

<400>  9

Met Lys Phe Met Ala Glu Asn Arg Leu Thr Leu Thr Lys Gly Thr Ala 
1               5                   10                  15      


Lys Asp Ile Ile Glu Arg Phe Tyr Thr Arg His Gly Ile Glu Thr Leu 
            20                  25                  30          


Glu Gly Phe Asp Gly Met Phe Val Thr Gln Thr Leu Glu Gln Glu Asp 
        35                  40                  45              


Phe Asp Glu Val Lys Ile Leu Thr Val Trp Lys Ser Lys Gln Ala Phe 
    50                  55                  60                  


Thr Asp Trp Leu Lys Ser Asp Val Phe Lys Ala Ala His Lys His Val 
65                  70                  75                  80  


Arg Ser Lys Asn Glu Asp Glu Ser Ser Pro Ile Ile Asn Asn Lys Val 
                85                  90                  95      


Ile Thr Tyr Asp Ile Gly Tyr Ser Tyr Met Lys 
            100                 105         


<210>  10
<211>  121
<212>  PRT
<213>  Listeria monocytogenes

<400>  10

Met Ile Ile Val Thr Asn Thr Ile Lys Val Glu Lys Gly Ala Ala Glu 
1               5                   10                  15      


His Val Ile Arg Gln Phe Thr Gly Ala Asn Gly Asp Gly His Pro Thr 
            20                  25                  30          


Lys Asp Ile Ala Glu Val Glu Gly Phe Leu Gly Phe Glu Leu Trp His 
        35                  40                  45              


Ser Lys Pro Glu Asp Lys Asp Tyr Glu Glu Val Val Val Thr Ser Lys 
    50                  55                  60                  


Trp Glu Ser Glu Glu Ala Gln Arg Asn Trp Val Lys Ser Asp Ser Phe 
65                  70                  75                  80  


Lys Lys Ala His Gly Arg Thr Lys Asp Thr Arg Glu Gln Arg Glu Asp 
                85                  90                  95      


Arg Lys Gly Ile Val Gly Asn Ala Ile Ala Arg Phe Glu Val Val His 
            100                 105                 110         


Val Gln Asn Pro Val Ile Val Glu Lys 
        115                 120     


<210>  11
<211>  105
<212>  PRT
<213>  Mycobacterium abscessus

<400>  11

Met Ser Val Val Lys Ile Asn Ala Ile Glu Ile Pro Glu Gly Ala Gly 
1               5                   10                  15      


Pro Glu Leu Glu Lys Arg Phe Ala His Arg Ala Gly Ala Val Glu Asn 
            20                  25                  30          


Gln Pro Gly Phe Leu Gly Phe Gln Leu Leu Arg Pro Val Lys Gly Glu 
        35                  40                  45              


Asp Arg Tyr Phe Val Val Thr Gln Trp Glu Ser Glu Glu Ala Phe Gln 
    50                  55                  60                  


Ala Trp Ala Thr Gly Pro Ala Val Glu Ala His Ala Gly Gln Gln Ala 
65                  70                  75                  80  


Lys Pro Val Ala Thr Gly Ala His Leu Leu Glu Phe Glu Val Val Leu 
                85                  90                  95      


Asp Val Thr Gly Ala Ala Ala Lys Ala 
            100                 105 


<210>  12
<211>  107
<212>  PRT
<213>  Bacillus cereus

<400>  12

Met Ile Ile Val Thr Asn Thr Ala Lys Ile Thr Lys Gly Asn Gly His 
1               5                   10                  15      


Lys Leu Ile Glu Arg Phe Asn Lys Val Gly Gln Val Glu Thr Met Pro 
            20                  25                  30          


Gly Phe Leu Gly Leu Glu Val Leu Leu Thr Gln Asn Thr Val Asp Tyr 
        35                  40                  45              


Asp Glu Val Thr Ile Ser Thr Arg Trp Asn Ala Lys Glu Asp Phe Gln 
    50                  55                  60                  


Gly Trp Thr Lys Ser Ser Ala Phe Lys Asp Ala His Ser His Gln Gly 
65                  70                  75                  80  


Gly Met Pro Glu Tyr Ile Leu Asp Asn Lys Ile Thr Tyr Tyr Asn Val 
                85                  90                  95      


Glu Val Val Arg Met Pro Met Ala Ala Ala Gln 
            100                 105         


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Forward primer to T7 promoter

<400>  13
taatacgact cactataggg                                                   20


<210>  14
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Reverse primer to terminator

<400>  14
gctagttatt gctcagcgg                                                    19
