                         SEQUENCE LISTING

<110>  MedImmune Limited
 
<120>  ENGINEERED O-GLYCOSYLATION IN RECOMBINANT POLYPEPTIDES AND USES 
       THEREOF

<130>  OGLYC-100-US-PSP

<160>  43    

<170>  PatentIn version 3.5

<210>  1
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i0

<400>  1

Glu Phe Ile Ala Trp Leu Val Lys Gly Ala Ala Ala Gly Gly Ser Gly 
1               5                   10                  15      


Ser Thr Ala Ser Ser Gly Ser Gly Ser Ala Thr 
            20                  25          


<210>  2
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i1

<400>  2

Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys Gln Ala 
1               5                   10                  15      


Gly Glu Ser 
            


<210>  3
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i1+10T

<400>  3

Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys Gln Thr 
1               5                   10                  15      


Gly Glu Ser 
            


<210>  4
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i2

<400>  4

Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys 
1               5                   10                  


<210>  5
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i1-11T


<220>
<221>  MISC_FEATURE
<222>  (2)..(6)
<223>  X is any amino acid

<400>  5

Thr Xaa Xaa Xaa Xaa Xaa Lys Asn Pro Leu Pro Thr Lys Glu Ala Ile 
1               5                   10                  15      


Glu Gln Glu Lys 
            20  


<210>  6
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i3

<400>  6

Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu 
1               5                   10      


<210>  7
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i4

<400>  7

Lys Asn Pro Leu Pro Thr Lys Glu Thr 
1               5                   


<210>  8
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i6

<400>  8

Pro Leu Pro Thr Lys Glu 
1               5       


<210>  9
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i6 +1A

<400>  9

Pro Leu Pro Thr Ala Glu 
1               5       


<210>  10
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i6 +3P

<400>  10

Pro Leu Pro Thr Lys Glu Pro 
1               5           


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i6 dbl

<400>  11

Pro Leu Pro Thr Ala Glu Pro 
1               5           


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i7 dbl

<400>  12

Pro Thr Ala Glu Pro 
1               5   


<210>  13
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  i8 P-1A

<400>  13

Ala Thr Ala Glu Pro 
1               5   


<210>  14
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-linked glycosylation sequence

<400>  14

Pro Thr Ala Glu Ala 
1               5   


<210>  15
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-linked glycosylation sequence

<400>  15

Ala Ala Pro Gly Pro Thr Pro Gly Pro 
1               5                   


<210>  16
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-linked glycosylation sequence

<400>  16

Ala Ala Val Gly Ala Thr Val Glu Gly 
1               5                   


<210>  17
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-linked glycosylation sequence

<400>  17

Ala Ala Asp Ser Thr Thr Pro Ala Pro 
1               5                   


<210>  18
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-linked glycosylation sequence

<400>  18

Ala Ala Ser Leu Pro Ser Ile Ser Ser 
1               5                   


<210>  19
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  19

Lys Gly Ala Ala Ala Gly Gly Ser Gly Ser Thr Ala Ser Ser Gly Ser 
1               5                   10                  15      


Gly Ser Ala Thr 
            20  


<210>  20
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  20

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys 
1               5                   10                  15      


Gln Ala Gly Glu Ser Ala Ala Ala Gly Ser Ser Gly Ser Gly Ser Ala 
            20                  25                  30          


Thr 
    


<210>  21
<211>  33
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  21

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys 
1               5                   10                  15      


Gln Thr Gly Glu Ser Ala Ala Ala Gly Ser Ser Gly Ser Gly Ser Ala 
            20                  25                  30          


Thr 
    


<210>  22
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  22

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys 
1               5                   10                  15      


Gly Ser Thr Ala Ser Ser Gly Ser Gly Ser Ala Thr 
            20                  25              


<210>  23
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  23

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys 
1               5                   10                  15      


Gly Ser Thr Ala Ser Ser Gly Ser Gly Ser Ala Thr 
            20                  25              


<210>  24
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  24

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Ala Ile Glu Gln Glu Lys 
1               5                   10                  15      


Gly Ser Thr Ala Ser Ser Gly Ser Gly Ser Ala Thr 
            20                  25              


<210>  25
<211>  28
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  25

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gln Glu Lys 
1               5                   10                  15      


Gly Ala Ala Ala Ser Ser Gly Ser Gly Ser Ala Thr 
            20                  25              


<210>  26
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  26

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Thr Ile Glu Gly Ser Thr 
1               5                   10                  15      


Ala Ser Ser Gly Ser Gly Ser Ala Thr 
            20                  25  


<210>  27
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  27

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Thr Gly Ser Thr Ala Ser 
1               5                   10                  15      


Ser Gly Ser Gly Ser Gly Ser Ala Thr 
            20                  25  


<210>  28
<211>  22
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  28

Lys Gly Lys Asn Pro Leu Pro Thr Lys Glu Gly Ser Thr Ala Ser Ser 
1               5                   10                  15      


Gly Ser Gly Ser Ala Thr 
            20          


<210>  29
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  29

Lys Gly Pro Leu Pro Thr Lys Glu Gly Ser Ala Thr 
1               5                   10          


<210>  30
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  30

Lys Gly Pro Leu Pro Thr Ala Glu Gly Ser Ala Thr 
1               5                   10          


<210>  31
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  31

Lys Gly Pro Leu Pro Thr Lys Glu Pro Gly Ser Ala Thr 
1               5                   10              


<210>  32
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  32

Lys Gly Pro Leu Pro Thr Ala Glu Pro Gly Ser Ala Thr 
1               5                   10              


<210>  33
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  33

Lys Gly Pro Thr Lys Glu Arg Gly Ser Ala Thr 
1               5                   10      


<210>  34
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  34

Lys Gly Pro Thr Ala Glu Pro Gly Ser Ala Thr 
1               5                   10      


<210>  35
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  35

Lys Gly Ala Thr Ala Glu Pro Gly Ser Thr Ala Ser Ser Gly Ser 
1               5                   10                  15  


<210>  36
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  36

Lys Gly Pro Thr Ala Glu Ala Gly Ser Thr Ala Ser Ser Gly Ser 
1               5                   10                  15  


<210>  37
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  37

Lys Gly Ala Ala Pro Gly Pro Thr Pro Gly Pro Gly Ser Thr Ala Ser 
1               5                   10                  15      


Ser Gly Ser Gly Ser Ala Thr 
            20              


<210>  38
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  38

Lys Gly Ala Ala Val Gly Ala Thr Val Glu Gly Gly Ser Thr Ala Ser 
1               5                   10                  15      


Ser Gly Ser Gly Ser Ala Thr 
            20              


<210>  39
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  39

Lys Gly Ala Ala Asp Ser Thr Thr Pro Ala Pro Gly Ser Thr Ala Ser 
1               5                   10                  15      


Ser Gly Ser Gly Ser Ala Thr 
            20              


<210>  40
<211>  23
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation of GLP-1:Fc fusion protein

<400>  40

Lys Gly Ala Ala Ser Leu Pro Ser Ile Ser Ser Gly Ser Thr Ala Ser 
1               5                   10                  15      


Ser Gly Ser Gly Ser Ala Thr 
            20              


<210>  41
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation sequence

<400>  41

Pro Thr Ala Glu Pro Gly 
1               5       


<210>  42
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  X is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(11)
<223>  X is any amino acid

<400>  42

Xaa Xaa Xaa Pro Ala Ala Glu Lys Xaa Xaa Xaa 
1               5                   10      


<210>  43
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  O-glycosylation sequence


<220>
<221>  MISC_FEATURE
<222>  (1)..(3)
<223>  X is any amino acid

<220>
<221>  MISC_FEATURE
<222>  (9)..(11)
<223>  X is any amino acid

<400>  43

Xaa Xaa Xaa Pro Thr Ala Glu Pro Xaa Xaa Xaa 
1               5                   10      


