                         SEQUENCE LISTING

<110>  Eli Lilly and Company
 
<120>  INCRETIN ANALOGS AND USES THEREOF

<130>  X21791

<150>  62/608,644
<151>  2017-12-21

<160>  12    

<170>  PatentIn version 3.5

<210>  1
<211>  29
<212>  PRT
<213>  Homo sapiens

<400>  1

His Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser 
1               5                   10                  15      


Arg Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr 
            20                  25                  


<210>  2
<211>  30
<212>  PRT
<213>  Homo sapiens

<400>  2

His Ala Glu Gly Thr Phe Thr Ser Asp Val Ser Ser Tyr Leu Glu Gly 
1               5                   10                  15      


Gln Ala Ala Lys Glu Phe Ile Ala Trp Leu Val Lys Gly Arg 
            20                  25                  30  


<210>  3
<211>  37
<212>  PRT
<213>  Homo sapiens

<400>  3

His Ser Gln Gly Thr Phe Thr Ser Asp Tyr Ser Lys Tyr Leu Asp Ser 
1               5                   10                  15      


Arg Arg Ala Gln Asp Phe Val Gln Trp Leu Met Asn Thr Lys Arg Asn 
            20                  25                  30          


Arg Asn Asn Ile Ala 
        35          


<210>  4
<211>  42
<212>  PRT
<213>  Homo sapiens

<400>  4

Tyr Ala Glu Gly Thr Phe Ile Ser Asp Tyr Ser Ile Ala Met Asp Lys 
1               5                   10                  15      


Ile His Gln Gln Asp Phe Val Asn Trp Leu Leu Ala Gln Lys Gly Lys 
            20                  25                  30          


Lys Asn Asp Trp Lys His Asn Ile Thr Gln 
        35                  40          


<210>  5
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is Aib

<220>
<221>  MISC_FEATURE
<222>  (3)..(3)
<223>  Xaa at position 3 is Gln or His

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is alpha-methyl-phenylalanine or 
       alpha-methyl-2-fluorophenylalanine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is alpha-methyl-Leu or Leu

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Xaa at position 17 is any amino acid with a functional group 
       available for conjugation wherein the functional group is 
       conjugated to a C16-C22 fatty acid

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa at position 2 is Aib, Gln, or His

<400>  5

Tyr Xaa Xaa Gly Thr Xaa Thr Ser Asp Tyr Ser Ile Xaa Leu Asp Lys 
1               5                   10                  15      


Xaa Ala Gln Xaa Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  6
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is alpha-amino-isobutyric acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is alpha-methyl-phenylalanine

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Lys at position 17 is chemically modified by conjugation of the 
       epsilon-amino group of the Lys side chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)-CO-(CH2)18-CO
       2H

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa at position 20 is alpha-amino-isobutyric acid

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  Ser at position 39 is optionally amidated

<400>  6

Tyr Xaa Gln Gly Thr Xaa Thr Ser Asp Tyr Ser Ile Leu Leu Asp Lys 
1               5                   10                  15      


Lys Ala Gln Xaa Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  7
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is alpha-amino-isobutyric acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is alpha-methyl-phenylalanine

<220>
<221>  MISC_FEATURE
<222>  (13)..(13)
<223>  Xaa at position 13 is alpha-methyl-Leu

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Lys at position 17 is chemically modified by conjugation of the 
       epsilon-amino group of the Lys side chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)-CO-(CH2)18-HC
       O2H

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa at position 2 is alpha-amino-isobutyric acid

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  Ser at position 39 is optionally amidated

<400>  7

Tyr Xaa Gln Gly Thr Xaa Thr Ser Asp Tyr Ser Ile Xaa Leu Asp Lys 
1               5                   10                  15      


Lys Ala Gln Xaa Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  8
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is alpha-amino-isobutyric acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is alpha-methyl-2-fluorophenylalanine

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Lys at position 17 is chemically modified by conjugation of the 
       epsilon-amino group of the Lys side chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)2-(gamma-Glu)-CO-(CH2)18-CO
       2H

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa at position 20 is alpha-amino-isobutyric acid

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  Ser at position 39 is optionally amidated

<400>  8

Tyr Xaa His Gly Thr Xaa Thr Ser Asp Tyr Ser Ile Leu Leu Asp Lys 
1               5                   10                  15      


Lys Ala Gln Xaa Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  9
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is alpha-amino-isobutyric acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is alpha-methyl-2-fluorophenylalanine

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Lys at position 17 is chemically modified by conjugation of the 
       epsilon-amino group of the Lys side chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)-(gamma-Glu)-CO-(CH2)18-CO2
       H

<220>
<221>  MISC_FEATURE
<222>  (20)..(20)
<223>  Xaa at position 20 is alpha-amino-isobutyric acid

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  Ser at position 39 is optionally amidated

<400>  9

Tyr Xaa Gln Gly Thr Xaa Thr Ser Asp Tyr Ser Ile Leu Leu Asp Lys 
1               5                   10                  15      


Lys Ala Gln Xaa Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  10
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is alpha-amino-isobutyric acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is alpha-methyl-2-fluorophenylalanine

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Lys at position 17 is chemically modified by conjugation of the 
       epsilon-amino group of the Lys side chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)-(gamma-Glu)-CO-(CH2)18-CO2
       H

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  Ser at position 39 is optionally amidated

<400>  10

Tyr Xaa Gln Gly Thr Xaa Thr Ser Asp Tyr Ser Ile Leu Leu Asp Lys 
1               5                   10                  15      


Lys Ala Gln Gln Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  11
<211>  39
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct


<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa at position 2 is alpha-amino-isobutyric acid

<220>
<221>  MISC_FEATURE
<222>  (6)..(6)
<223>  Xaa at position 6 is alpha-methyl-2-fluorophenylalanine

<220>
<221>  MOD_RES
<222>  (17)..(17)
<223>  Lys at position 17 is chemically modified by conjugation of the 
       epsilon-amino group of the Lys side chain with 
       (2-[2-(2-Amino-ethoxy)-ethoxy]-acetyl)-(gamma-Glu)-CO-(CH2)18-CO2
       H

<220>
<221>  MOD_RES
<222>  (39)..(39)
<223>  Ser at position 39 is optionally amidated

<400>  11

Tyr Xaa Gln Gly Thr Xaa Thr Ser Asp Tyr Ser Ile Leu Leu Asp Lys 
1               5                   10                  15      


Lys Ala Gln His Ala Phe Ile Glu Tyr Leu Leu Glu Gly Gly Pro Ser 
            20                  25                  30          


Ser Gly Ala Pro Pro Pro Ser 
        35                  


<210>  12
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic Construct

<400>  12

Gly Pro Ser Ser Gly Ala Pro Pro Pro Ser 
1               5                   10  


