                         SEQUENCE LISTING

<110>  MASSACHUSETTS INSTITUTE OF TECHNOLOGY
       THE SCRIPPS RESEARCH INSTITUTE
       IRVINE, Darrell J.
       MOYER, Tyson
       SCHIEF, William
 
<120>  ANTIGEN-ADJUVANT COUPLING REAGENTS AND METHODS OF USE

<130>  MITN-044PC

<140>  PCT/US2018/066576
<141>  2018-12-19

<150>  US 62/607,691
<151>  2017-12-19

<160>  14    

<170>  PatentIn version 3.5

<210>  1
<211>  198
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: amino acid sequence of eOD

<400>  1

Thr Gly Cys His His His His His His Gly Gly Asp Thr Ile Thr Leu 
1               5                   10                  15      


Pro Cys Arg Pro Ala Pro Pro Pro His Cys Ser Ser Asn Ile Thr Gly 
            20                  25                  30          


Leu Ile Leu Thr Arg Gln Gly Gly Tyr Ser Asn Asp Asn Thr Val Ile 
        35                  40                  45              


Phe Arg Pro Ser Gly Gly Asp Trp Arg Asp Ile Ala Arg Cys Gln Ile 
    50                  55                  60                  


Ala Gly Thr Val Val Ser Thr Gln Leu Phe Leu Asn Gly Ser Leu Ala 
65                  70                  75                  80  


Glu Glu Glu Val Val Ile Arg Ser Glu Asp Trp Arg Asp Asn Ala Lys 
                85                  90                  95      


Ser Ile Cys Val Gln Leu Asn Thr Ser Val Glu Ile Asn Cys Thr Gly 
            100                 105                 110         


Ala Gly His Cys Asn Ile Ser Arg Ala Lys Trp Asn Asn Thr Leu Lys 
        115                 120                 125             


Gln Ile Ala Ser Lys Leu Arg Glu Gln Tyr Gly Asn Lys Thr Ile Ile 
    130                 135                 140                 


Phe Lys Pro Ser Ser Gly Gly Asp Pro Glu Phe Val Asn His Ser Phe 
145                 150                 155                 160 


Asn Cys Gly Gly Glu Phe Phe Tyr Cys Asp Ser Thr Gln Leu Phe Asn 
                165                 170                 175     


Ser Thr Trp Phe Asn Ser Thr Gly Ser Ala Phe Lys Val Ala Ala Trp 
            180                 185                 190         


Thr Leu Lys Ala Ala Ala 
        195             


<210>  2
<211>  646
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic: amino acid sequence of the cysteine-modified SOSIP

<400>  2

Thr Gly Ala Glu Asn Leu Trp Val Thr Val Tyr Tyr Gly Val Pro Val 
1               5                   10                  15      


Trp Lys Asp Ala Glu Thr Thr Leu Phe Cys Ala Ser Asp Ala Lys Ala 
            20                  25                  30          


Tyr Glu Thr Glu Lys His Asn Val Trp Ala Thr His Ala Cys Val Pro 
        35                  40                  45              


Thr Asp Pro Asn Pro Gln Glu Ile His Leu Glu Asn Val Thr Glu Glu 
    50                  55                  60                  


Phe Asn Met Trp Lys Asn Asn Met Val Glu Gln Met His Thr Asp Ile 
65                  70                  75                  80  


Ile Ser Leu Trp Asp Gln Ser Leu Lys Pro Cys Val Lys Leu Thr Pro 
                85                  90                  95      


Leu Cys Val Thr Leu Gln Cys Thr Asn Val Thr Asn Asn Ile Thr Asp 
            100                 105                 110         


Asp Met Arg Gly Glu Leu Lys Asn Cys Ser Phe Asn Met Thr Thr Glu 
        115                 120                 125             


Leu Arg Asp Lys Lys Gln Lys Val Tyr Ser Leu Phe Tyr Arg Leu Asp 
    130                 135                 140                 


Val Val Gln Ile Asn Glu Asn Gln Gly Asn Arg Ser Asn Asn Ser Asn 
145                 150                 155                 160 


Lys Glu Tyr Arg Leu Ile Asn Cys Asn Thr Ser Ala Ile Thr Gln Ala 
                165                 170                 175     


Cys Pro Lys Val Ser Phe Glu Pro Ile Pro Ile His Tyr Cys Ala Pro 
            180                 185                 190         


Ala Gly Phe Ala Ile Leu Lys Cys Lys Asp Lys Lys Phe Asn Gly Thr 
        195                 200                 205             


Gly Pro Cys Pro Ser Val Ser Thr Val Gln Cys Thr His Gly Ile Lys 
    210                 215                 220                 


Pro Val Val Ser Thr Gln Leu Leu Leu Asn Gly Ser Leu Ala Glu Glu 
225                 230                 235                 240 


Glu Val Met Ile Arg Ser Glu Asn Ile Thr Asn Asn Ala Lys Asn Ile 
                245                 250                 255     


Leu Val Gln Phe Asn Thr Pro Val Gln Ile Asn Cys Thr Arg Pro Asn 
            260                 265                 270         


Asn Asn Thr Arg Lys Ser Ile Arg Ile Gly Pro Gly Gln Ala Phe Tyr 
        275                 280                 285             


Ala Thr Gly Asp Ile Ile Gly Asp Ile Arg Gln Ala His Cys Asn Val 
    290                 295                 300                 


Ser Lys Ala Thr Trp Asn Glu Thr Leu Gly Lys Val Val Lys Gln Leu 
305                 310                 315                 320 


Arg Lys His Phe Gly Asn Asn Thr Ile Ile Arg Phe Ala Asn Ser Ser 
                325                 330                 335     


Gly Gly Asp Leu Glu Val Thr Thr His Ser Phe Asn Cys Gly Gly Glu 
            340                 345                 350         


Phe Phe Tyr Cys Asn Thr Ser Gly Leu Phe Asn Ser Thr Trp Ile Ser 
        355                 360                 365             


Asn Thr Ser Val Gln Gly Ser Asn Ser Thr Gly Ser Asn Asp Ser Ile 
    370                 375                 380                 


Thr Leu Pro Cys Arg Ile Lys Gln Ile Ile Asn Met Trp Gln Arg Ile 
385                 390                 395                 400 


Gly Gln Ala Met Tyr Ala Pro Pro Ile Gln Gly Val Ile Arg Cys Val 
                405                 410                 415     


Ser Asn Ile Thr Gly Leu Ile Leu Thr Arg Asp Gly Gly Ser Thr Asn 
            420                 425                 430         


Ser Thr Thr Glu Thr Phe Arg Pro Gly Gly Gly Asp Met Arg Asp Asn 
        435                 440                 445             


Trp Arg Ser Glu Leu Tyr Lys Tyr Lys Val Val Lys Ile Glu Pro Leu 
    450                 455                 460                 


Gly Val Ala Pro Thr Arg Cys Lys Arg Arg Val Val Gly Arg Arg Arg 
465                 470                 475                 480 


Arg Arg Arg Ala Val Gly Ile Gly Ala Val Phe Leu Gly Phe Leu Gly 
                485                 490                 495     


Ala Ala Gly Ser Thr Met Gly Ala Ala Ser Met Thr Leu Thr Val Gln 
            500                 505                 510         


Ala Arg Asn Leu Leu Ser Gly Ile Val Gln Gln Gln Ser Asn Leu Leu 
        515                 520                 525             


Arg Ala Pro Glu Ala Gln Gln His Leu Leu Lys Leu Thr Val Trp Gly 
    530                 535                 540                 


Ile Lys Gln Leu Gln Ala Arg Val Leu Ala Val Glu Arg Tyr Leu Arg 
545                 550                 555                 560 


Asp Gln Gln Leu Leu Gly Ile Trp Gly Cys Ser Gly Lys Leu Ile Cys 
                565                 570                 575     


Cys Thr Asn Val Pro Trp Asn Ser Ser Trp Ser Asn Arg Asn Leu Ser 
            580                 585                 590         


Glu Ile Trp Asp Asn Met Thr Trp Leu Gln Trp Asp Lys Glu Ile Ser 
        595                 600                 605             


Asn Tyr Thr Gln Ile Ile Tyr Gly Leu Leu Glu Glu Ser Gln Asn Gln 
    610                 615                 620                 


Gln Glu Lys Asn Glu Gln Asp Leu Leu Ala Leu Asp Gly Thr Lys His 
625                 630                 635                 640 


His His His His His Cys 
                645     


<210>  3
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker


<220>
<221>  misc_feature
<222>  (6)..(25)
<223>  Any "Gly Gly Gly Ser" group may be present or absent

<400>  3

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  4
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  4

Gly Gly Gly Gly Ser 
1               5   


<210>  5
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  5

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10  


<210>  6
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  6

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  7
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  7

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser 
            20  


<210>  8
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  8

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser 
            20                  25  


<210>  9
<211>  27
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker


<220>
<221>  misc_feature
<222>  (12)..(26)
<223>  Any "Glu Ala Ala Ala Lys" group may be present or absent

<400>  9

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
            20                  25          


<210>  10
<211>  50
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  10

Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala 
1               5                   10                  15      


Ala Lys Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys 
            20                  25                  30          


Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45              


Leu Glu 
    50  


<210>  11
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  11

Gly Gly Ser Gly 
1               


<210>  12
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker


<220>
<221>  misc_feature
<222>  (5)..(20)
<223>  Any "Gly Gly Ser Gly" group may be present or absent

<400>  12

Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Ser Gly 
            20  


<210>  13
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker

<400>  13

Gly Ser Ala Thr 
1               


<210>  14
<211>  30
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Synthetic linker


<220>
<221>  misc_feature
<222>  (7)..(30)
<223>  Any "Gly Gly Ser Gly Gly Ser" group may be present or absent

<400>  14

Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly 
1               5                   10                  15      


Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser Gly Gly Ser 
            20                  25                  30  


