                         SEQUENCE LISTING

<110>  DANISCO
 
<120>  MUTANT AND GENETICALLY MODIFIED BACILLUS CELLS AND METHODS 
       THEREOF FOR INCREASED PROTEIN PRODUCTION

<130>  NB41301-WO-PCT

<160>  84    

<170>  PatentIn version 3.5

<210>  1
<211>  4188
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  synthetic polynucleotide encoding Cas9

<400>  1
gtggccccaa aaaagaaacg caaggttatg gataaaaaat acagcattgg tctggatatc       60

ggaaccaaca gcgttgggtg ggcagtaata acagatgaat acaaagtgcc gtcaaaaaaa      120

tttaaggttc tggggaatac agatcgccac agcataaaaa agaatctgat tggggcattg      180

ctgtttgatt cgggtgagac agctgaggcc acgcgtctga aacgtacagc aagaagacgt      240

tacacacgtc gtaaaaatcg tatttgctac ttacaggaaa ttttttctaa cgaaatggcc      300

aaggtagatg atagtttctt ccatcgtctc gaagaatctt ttctggttga ggaagataaa      360

aaacacgaac gtcaccctat ctttggcaat atcgtggatg aagtggccta tcatgaaaaa      420

taccctacga tttatcatct tcgcaagaag ttggttgata gtacggacaa agcggatctg      480

cgtttaatct atcttgcgtt agcgcacatg atcaaatttc gtggtcattt cttaattgaa      540

ggtgatctga atcctgataa ctctgatgtg gacaaattgt ttatacaatt agtgcaaacc      600

tataatcagc tgttcgagga aaaccccatt aatgcctctg gagttgatgc caaagcgatt      660

ttaagcgcga gactttctaa gtcccggcgt ctggagaatc tgatcgccca gttaccaggg      720

gaaaagaaaa atggtctgtt tggtaatctg attgccctca gtctggggct taccccgaac      780

ttcaaatcca attttgacct ggctgaggac gcaaagctgc agctgagcaa agatacttat      840

gatgatgacc tcgacaatct gctcgcccag attggtgacc aatatgcgga tctgtttctg      900

gcagcgaaga atctttcgga tgctatcttg ctgtcggata ttctgcgtgt taataccgaa      960

atcaccaaag cgcctctgtc tgcaagtatg atcaagagat acgacgagca ccaccaggac     1020

ctgactcttc ttaaggcact ggtacgccaa cagcttccgg agaaatacaa agaaatattc     1080

ttcgaccagt ccaagaatgg ttacgcgggc tacatcgatg gtggtgcatc acaggaagag     1140

ttctataaat ttattaaacc aatccttgag aaaatggatg gcacggaaga gttacttgtt     1200

aaacttaacc gcgaagactt gcttagaaag caacgtacat tcgacaacgg ctccatccca     1260

caccagattc atttaggtga acttcacgcc atcttgcgca gacaagaaga tttctatccc     1320

ttcttaaaag acaatcggga gaaaatcgag aagatcctga cgttccgcat tccctattat     1380

gtcggtcccc tggcacgtgg taattctcgg tttgcctgga tgacgcgcaa aagtgaggaa     1440

accatcaccc cttggaactt tgaagaagtc gtggataaag gtgctagcgc gcagtctttt     1500

atagaaagaa tgacgaactt cgataaaaac ttgcccaacg aaaaagtcct gcccaagcac     1560

tctcttttat atgagtactt tactgtgtac aacgaactga ctaaagtgaa atacgttacg     1620

gaaggtatgc gcaaacctgc ctttcttagt ggcgagcaga aaaaagcaat tgtcgatctt     1680

ctctttaaaa cgaatcgcaa ggtaactgta aaacagctga aggaagatta tttcaaaaag     1740

atcgaatgct ttgattctgt cgagatctcg ggtgtcgaag atcgtttcaa cgcttcctta     1800

gggacctatc atgatttgct gaagataata aaagacaaag actttctcga caatgaagaa     1860

aatgaagata ttctggagga tattgttttg accttgacct tattcgaaga tagagagatg     1920

atcgaggagc gcttaaaaac ctatgcccac ctgtttgatg acaaagtcat gaagcaatta     1980

aagcgccgca gatatacggg gtggggccgc ttgagccgca agttgattaa cggtattaga     2040

gacaagcaga gcggaaaaac tatcctggat ttcctcaaat ctgacggatt tgcgaaccgc     2100

aattttatgc agcttataca tgatgattcg cttacattca aagaggatat tcagaaggct     2160

caggtgtctg ggcaaggtga ttcactccac gaacatatag caaatttggc cggctctcct     2220

gcgattaaga aggggatcct gcaaacagtt aaagttgtgg atgaacttgt aaaagtaatg     2280

ggccgccaca agccggagaa tatcgtgata gaaatggcgc gcgagaatca aacgacacaa     2340

aaaggtcaaa agaactcaag agagagaatg aagcgcattg aggaggggat aaaggaactt     2400

ggatctcaaa ttctgaaaga acatccagtt gaaaacactc agctgcaaaa tgaaaaattg     2460

tacctgtact acctgcagaa tggaagagac atgtacgtgg atcaggaatt ggatatcaat     2520

agactctcgg actatgacgt agatcacatt gtccctcaga gcttcctcaa ggatgattct     2580

atagataata aagtacttac gagatcggac aaaaatcgcg gtaaatcgga taacgtccca     2640

tcggaggaag tcgttaaaaa gatgaaaaac tattggcgtc aactgctgaa cgccaagctg     2700

atcacacagc gtaagtttga taatctgact aaagccgaac gcggtggtct tagtgaactc     2760

gataaagcag gatttataaa acggcagtta gtagaaacgc gccaaattac gaaacacgtg     2820

gctcagatcc tcgattctag aatgaataca aagtacgatg aaaacgataa actgatccgt     2880

gaagtaaaag tcattacctt aaaatctaaa cttgtgtccg atttccgcaa agattttcag     2940

ttttacaagg tccgggaaat caataactat caccatgcac atgatgcata tttaaatgcg     3000

gttgtaggca cggcccttat taagaaatac cctaaactcg aaagtgagtt tgtttatggg     3060

gattataaag tgtatgacgt tcgcaaaatg atcgcgaaat cagaacagga aatcggtaag     3120

gctaccgcta aatacttttt ttattccaac attatgaatt tttttaagac cgaaataact     3180

ctcgcgaatg gtgaaatccg taaacggcct cttatagaaa ccaatggtga aacgggagaa     3240

atcgtttggg ataaaggtcg tgactttgcc accgttcgta aagtcctctc aatgccgcaa     3300

gttaacattg tcaagaagac ggaagttcaa acagggggat tctccaaaga atctatcctg     3360

ccgaagcgta acagtgataa acttattgcc agaaaaaaag attgggatcc aaaaaaatac     3420

ggaggctttg attcccctac cgtcgcgtat agtgtgctgg tggttgctaa agtcgagaaa     3480

gggaaaagca agaaattgaa atcagttaaa gaactgctgg gtattacaat tatggaaaga     3540

tcgtcctttg agaaaaatcc gatcgacttt ttagaggcca aggggtataa ggaagtgaaa     3600

aaagatctca tcatcaaatt accgaagtat agtctttttg agctggaaaa cggcagaaaa     3660

agaatgctgg cctccgcggg cgagttacag aagggaaatg agctggcgct gccttccaaa     3720

tatgttaatt ttctgtacct tgccagtcat tatgagaaac tgaagggcag ccccgaagat     3780

aacgaacaga aacaattatt cgtggaacag cataagcact atttagatga aattatagag     3840

caaattagtg aattttctaa gcgcgttatc ctcgcggatg ctaatttaga caaagtactg     3900

tcagcttata ataaacatcg ggataagccg attagagaac aggccgaaaa tatcattcat     3960

ttgtttacct taaccaacct tggagcacca gctgccttca aatatttcga taccacaatt     4020

gatcgtaaac ggtatacaag tacaaaagaa gtcttggacg caaccctcat tcatcaatct     4080

attactggat tatatgagac acgcattgat ctttcacagc tgggcggaga caagaagaaa     4140

aaactgaaac tgcaccatca tcaccatcat catcaccatc attgataa                  4188


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  N-terminal NLS

<400>  2

Ala Pro Lys Lys Lys Arg Lys Val 
1               5               


<210>  3
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C-terminal NLS

<400>  3

Lys Lys Lys Lys Leu Lys 
1               5       


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  10-histidine tag

<400>  4

His His His His His His His His His His 
1               5                   10  


<210>  5
<211>  607
<212>  DNA
<213>  Bacillus subtilis

<400>  5
attcctccat tttcttctgc tatcaaaata acagactcgt gattttccaa acgagctttc       60

aaaaaagcct ctgccccttg caaatcggat gcctgtctat aaaattcccg atattggtta      120

aacagcggcg caatggcggc cgcatctgat gtctttgctt ggcgaatgtt catcttattt      180

cttcctccct ctcaataatt ttttcattct atcccttttc tgtaaagttt atttttcaga      240

atacttttat catcatgctt tgaaaaaata tcacgataat atccattgtt ctcacggaag      300

cacacgcagg tcatttgaac gaattttttc gacaggaatt tgccgggact caggagcatt      360

taacctaaaa aagcatgaca tttcagcata atgaacattt actcatgtct attttcgttc      420

ttttctgtat gaaaatagtt atttcgagtc tctacggaaa tagcgagaga tgatatacct      480

aaatagagat aaaatcatct caaaaaaatg ggtctactaa aatattattc catctattac      540

aataaattca cagaatagtc ttttaagtaa gtctactctg aattttttta aaaggagagg      600

gtaacta                                                                607


<210>  6
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward primer

<400>  6
atatatgagt aaacttggtc tgacagaatt cctccatttt cttctgctat                  50


<210>  7
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 reverse primer

<400>  7
tgcggccgcg aattcgatta cgaatgccgt ctccc                                  35


<210>  8
<211>  3290
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKB320 backbone

<400>  8
gaattcgcgg ccgcacgcgt ccatggggat ccccgcgggt cgacctcgag agttacgcta       60

gggataacag ggtaatatag gagctccagt cggcttaaac cagttttcgc tggtgcgaaa      120

aaagagtgtc ttgtgacacc taaattcaaa atctatcggt cagatttata ccgatttgat      180

tttatatatt cttgaataac atacgccgag ttatcacata aaagcgggaa ccaatcataa      240

aatttaaact tcattgcata atccattaaa ctcttaaatt ctacgattcc ttgttcatca      300

ataaactcaa tcatttcttt aattaattta tatctatctg ttgttgtttt ctttaataat      360

tcattaacat ctacaccgcc ataaactatc atatcttctt tttgatattt aaatttatta      420

ggatcgtcca tgtgaagcat atatctcaca agacctttca cacttcctgc aatctgcgga      480

atagtcgcat tcaattcttc tgttaattat ttttatctgt tcataagatt tattaccctc      540

atacatcact agaatatgat aatgctcttt tttcatccta ccttctgtat cagtatccct      600

atcatgtaat ggagacacta caaattgaat gtgtaactct tttaaatact ctaaccactc      660

ggcttttgct gattctggat ataaaacaaa tgtccaatta cgtcctcttg aatttttctt      720

gttttcagtt tcttttatta cattttcgct catgatataa taacggtgct aatacactta      780

acaaaattta gtcatagata ggcagcatgc cagtgctgtc tatctttttt tgtttaaaat      840

gcaccgtatt cctcctttgc atattttttt attagaatac cggttgcatc tgatttgcta      900

atattatatt tttctttgat tctatttaat atctcatttt cttctgttgt aagtcttaaa      960

gtaacagcaa cttttttctc ttcttttcta tctacaacta tcactgtacc tcccaacatc     1020

tgtttttttc actttaacat aaaaaacaac cttttaacat taaaaaccca atatttattt     1080

atttgtttgg acaatggaca ctggacacct aggggggagg tcgtagtacc cccctatgtt     1140

ttctccccta aataacccca aaaatctaag aaaaaaagac ctcaaaaagg tctttaatta     1200

acatctcaaa tttcgcattt attccaattt cctttttgcg tgtgatgcga gctcatcggc     1260

tccgtcgata ctatgttata cgccaacttt caaaacaact ttgaaaaagc tgttttctgg     1320

tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct     1380

tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga     1440

gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag     1500

gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa     1560

tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc     1620

tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct     1680

ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg     1740

aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca     1800

tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt     1860

acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat     1920

ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg     1980

tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg     2040

gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg     2100

tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac     2160

tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt     2220

agtgactgca gtgagatctg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag     2280

gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg     2340

agtaggacaa atccgccgct ctagctaagc agaaggccat cctgacggat ggcctttttg     2400

cgtttctaca aactcttgtt aactctagag ctgcctgccg cgtttcggtg atgaagatct     2460

tcccgatgat taattaattc agaacgctcg gttgccgccg ggcgtttttt atgaagcttc     2520

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     2580

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     2640

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     2700

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     2760

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     2820

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     2880

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     2940

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     3000

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     3060

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     3120

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     3180

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     3240

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca                3290


<210>  9
<211>  4204
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKB320

<400>  9
gcggccgcac gcgtccatgg ggatccccgc gggtcgacct cgagagttac gctagggata       60

acagggtaat ataggagctc cagtcggctt aaaccagttt tcgctggtgc gaaaaaagag      120

tgtcttgtga cacctaaatt caaaatctat cggtcagatt tataccgatt tgattttata      180

tattcttgaa taacatacgc cgagttatca cataaaagcg ggaaccaatc ataaaattta      240

aacttcattg cataatccat taaactctta aattctacga ttccttgttc atcaataaac      300

tcaatcattt ctttaattaa tttatatcta tctgttgttg ttttctttaa taattcatta      360

acatctacac cgccataaac tatcatatct tctttttgat atttaaattt attaggatcg      420

tccatgtgaa gcatatatct cacaagacct ttcacacttc ctgcaatctg cggaatagtc      480

gcattcaatt cttctgttaa ttatttttat ctgttcataa gatttattac cctcatacat      540

cactagaata tgataatgct cttttttcat cctaccttct gtatcagtat ccctatcatg      600

taatggagac actacaaatt gaatgtgtaa ctcttttaaa tactctaacc actcggcttt      660

tgctgattct ggatataaaa caaatgtcca attacgtcct cttgaatttt tcttgttttc      720

agtttctttt attacatttt cgctcatgat ataataacgg tgctaataca cttaacaaaa      780

tttagtcata gataggcagc atgccagtgc tgtctatctt tttttgttta aaatgcaccg      840

tattcctcct ttgcatattt ttttattaga ataccggttg catctgattt gctaatatta      900

tatttttctt tgattctatt taatatctca ttttcttctg ttgtaagtct taaagtaaca      960

gcaacttttt tctcttcttt tctatctaca actatcactg tacctcccaa catctgtttt     1020

tttcacttta acataaaaaa caacctttta acattaaaaa cccaatattt atttatttgt     1080

ttggacaatg gacactggac acctaggggg gaggtcgtag taccccccta tgttttctcc     1140

cctaaataac cccaaaaatc taagaaaaaa agacctcaaa aaggtcttta attaacatct     1200

caaatttcgc atttattcca atttcctttt tgcgtgtgat gcgagctcat cggctccgtc     1260

gatactatgt tatacgccaa ctttcaaaac aactttgaaa aagctgtttt ctggtattta     1320

aggttttaga atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg     1380

ggtatcttta aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat     1440

caccggaatt gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt     1500

ctcctgctaa ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg     1560

acagccggta taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc     1620

tggaaggaaa gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca     1680

atctgctcat gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa     1740

gccctgaaaa gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca     1800

tatcggattg tccctatacg aatagcttag acagccgctt agccgaattg gattacttac     1860

tgaataacga tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag     1920

atccgcgcga gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt     1980

cccacggcga cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta     2040

ttgatcttgg gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt     2100

cgatcaggga ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga     2160

tcaagcctga ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtgac     2220

tgcagtgaga tctggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag     2280

tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg     2340

acaaatccgc cgctctagct aagcagaagg ccatcctgac ggatggcctt tttgcgtttc     2400

tacaaactct tgttaactct agagctgcct gccgcgtttc ggtgatgaag atcttcccga     2460

tgattaatta attcagaacg ctcggttgcc gccgggcgtt ttttatgaag cttcgttgct     2520

ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca     2580

gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct     2640

cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc     2700

gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt     2760

tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc     2820

cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc     2880

cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg     2940

gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc     3000

agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag     3060

cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga     3120

tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat     3180

tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag     3240

ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat     3300

cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc     3360

cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat     3420

accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag     3480

ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg     3540

ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc     3600

tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca     3660

acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg     3720

tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc     3780

actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta     3840

ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc     3900

aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg     3960

ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc     4020

cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc     4080

aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat     4140

actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgga     4200

attc                                                                  4204


<210>  10
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKB320 forward primer

<400>  10
gggagacggc attcgtaatc gaattcgcgg ccgca                                  35


<210>  11
<211>  50
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pKB320 reverse primer

<400>  11
atagcagaag aaaatggagg aattctgtca gaccaagttt actcatatat                  50


<210>  12
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 reverse sequencing primer

<400>  12
ccgactggag ctcctatatt acc                                               23


<210>  13
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 reverse sequening primer 2

<400>  13
gctgtggcga tctgtattcc                                                   20


<210>  14
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward sequencing primer 1

<400>  14
gtcttttaag taagtctact ct                                                22


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward seq primer 2

<400>  15
ccaaagcgat tttaagcgcg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward seq primer 3

<400>  16
cctggcacgt ggtaattctc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward seq primer 4

<400>  17
ggatttcctc aaatctgacg                                                   20


<210>  18
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward seq primer 5

<400>  18
gtagaaacgc gccaaattac g                                                 21


<210>  19
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward seq primer 6

<400>  19
gctggtggtt gctaaagtcg                                                   20


<210>  20
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 forward seq primer 7

<400>  20
ggacgcaacc ctcattcatc                                                   20


<210>  21
<211>  8347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRF694

<400>  21
gaattcctcc attttcttct gctatcaaaa taacagactc gtgattttcc aaacgagctt       60

tcaaaaaagc ctctgcccct tgcaaatcgg atgcctgtct ataaaattcc cgatattggt      120

taaacagcgg cgcaatggcg gccgcatctg atgtctttgc ttggcgaatg ttcatcttat      180

ttcttcctcc ctctcaataa ttttttcatt ctatcccttt tctgtaaagt ttatttttca      240

gaatactttt atcatcatgc tttgaaaaaa tatcacgata atatccattg ttctcacgga      300

agcacacgca ggtcatttga acgaattttt tcgacaggaa tttgccggga ctcaggagca      360

tttaacctaa aaaagcatga catttcagca taatgaacat ttactcatgt ctattttcgt      420

tcttttctgt atgaaaatag ttatttcgag tctctacgga aatagcgaga gatgatatac      480

ctaaatagag ataaaatcat ctcaaaaaaa tgggtctact aaaatattat tccatctatt      540

acaataaatt cacagaatag tcttttaagt aagtctactc tgaatttttt taaaaggaga      600

gggtaactag tggccccaaa aaagaaacgc aaggttatgg ataaaaaata cagcattggt      660

ctggatatcg gaaccaacag cgttgggtgg gcagtaataa cagatgaata caaagtgccg      720

tcaaaaaaat ttaaggttct ggggaataca gatcgccaca gcataaaaaa gaatctgatt      780

ggggcattgc tgtttgattc gggtgagaca gctgaggcca cgcgtctgaa acgtacagca      840

agaagacgtt acacacgtcg taaaaatcgt atttgctact tacaggaaat tttttctaac      900

gaaatggcca aggtagatga tagtttcttc catcgtctcg aagaatcttt tctggttgag      960

gaagataaaa aacacgaacg tcaccctatc tttggcaata tcgtggatga agtggcctat     1020

catgaaaaat accctacgat ttatcatctt cgcaagaagt tggttgatag tacggacaaa     1080

gcggatctgc gtttaatcta tcttgcgtta gcgcacatga tcaaatttcg tggtcatttc     1140

ttaattgaag gtgatctgaa tcctgataac tctgatgtgg acaaattgtt tatacaatta     1200

gtgcaaacct ataatcagct gttcgaggaa aaccccatta atgcctctgg agttgatgcc     1260

aaagcgattt taagcgcgag actttctaag tcccggcgtc tggagaatct gatcgcccag     1320

ttaccagggg aaaagaaaaa tggtctgttt ggtaatctga ttgccctcag tctggggctt     1380

accccgaact tcaaatccaa ttttgacctg gctgaggacg caaagctgca gctgagcaaa     1440

gatacttatg atgatgacct cgacaatctg ctcgcccaga ttggtgacca atatgcggat     1500

ctgtttctgg cagcgaagaa tctttcggat gctatcttgc tgtcggatat tctgcgtgtt     1560

aataccgaaa tcaccaaagc gcctctgtct gcaagtatga tcaagagata cgacgagcac     1620

caccaggacc tgactcttct taaggcactg gtacgccaac agcttccgga gaaatacaaa     1680

gaaatattct tcgaccagtc caagaatggt tacgcgggct acatcgatgg tggtgcatca     1740

caggaagagt tctataaatt tattaaacca atccttgaga aaatggatgg cacggaagag     1800

ttacttgtta aacttaaccg cgaagacttg cttagaaagc aacgtacatt cgacaacggc     1860

tccatcccac accagattca tttaggtgaa cttcacgcca tcttgcgcag acaagaagat     1920

ttctatccct tcttaaaaga caatcgggag aaaatcgaga agatcctgac gttccgcatt     1980

ccctattatg tcggtcccct ggcacgtggt aattctcggt ttgcctggat gacgcgcaaa     2040

agtgaggaaa ccatcacccc ttggaacttt gaagaagtcg tggataaagg tgctagcgcg     2100

cagtctttta tagaaagaat gacgaacttc gataaaaact tgcccaacga aaaagtcctg     2160

cccaagcact ctcttttata tgagtacttt actgtgtaca acgaactgac taaagtgaaa     2220

tacgttacgg aaggtatgcg caaacctgcc tttcttagtg gcgagcagaa aaaagcaatt     2280

gtcgatcttc tctttaaaac gaatcgcaag gtaactgtaa aacagctgaa ggaagattat     2340

ttcaaaaaga tcgaatgctt tgattctgtc gagatctcgg gtgtcgaaga tcgtttcaac     2400

gcttccttag ggacctatca tgatttgctg aagataataa aagacaaaga ctttctcgac     2460

aatgaagaaa atgaagatat tctggaggat attgttttga ccttgacctt attcgaagat     2520

agagagatga tcgaggagcg cttaaaaacc tatgcccacc tgtttgatga caaagtcatg     2580

aagcaattaa agcgccgcag atatacgggg tggggccgct tgagccgcaa gttgattaac     2640

ggtattagag acaagcagag cggaaaaact atcctggatt tcctcaaatc tgacggattt     2700

gcgaaccgca attttatgca gcttatacat gatgattcgc ttacattcaa agaggatatt     2760

cagaaggctc aggtgtctgg gcaaggtgat tcactccacg aacatatagc aaatttggcc     2820

ggctctcctg cgattaagaa ggggatcctg caaacagtta aagttgtgga tgaacttgta     2880

aaagtaatgg gccgccacaa gccggagaat atcgtgatag aaatggcgcg cgagaatcaa     2940

acgacacaaa aaggtcaaaa gaactcaaga gagagaatga agcgcattga ggaggggata     3000

aaggaacttg gatctcaaat tctgaaagaa catccagttg aaaacactca gctgcaaaat     3060

gaaaaattgt acctgtacta cctgcagaat ggaagagaca tgtacgtgga tcaggaattg     3120

gatatcaata gactctcgga ctatgacgta gatcacattg tccctcagag cttcctcaag     3180

gatgattcta tagataataa agtacttacg agatcggaca aaaatcgcgg taaatcggat     3240

aacgtcccat cggaggaagt cgttaaaaag atgaaaaact attggcgtca actgctgaac     3300

gccaagctga tcacacagcg taagtttgat aatctgacta aagccgaacg cggtggtctt     3360

agtgaactcg ataaagcagg atttataaaa cggcagttag tagaaacgcg ccaaattacg     3420

aaacacgtgg ctcagatcct cgattctaga atgaatacaa agtacgatga aaacgataaa     3480

ctgatccgtg aagtaaaagt cattacctta aaatctaaac ttgtgtccga tttccgcaaa     3540

gattttcagt tttacaaggt ccgggaaatc aataactatc accatgcaca tgatgcatat     3600

ttaaatgcgg ttgtaggcac ggcccttatt aagaaatacc ctaaactcga aagtgagttt     3660

gtttatgggg attataaagt gtatgacgtt cgcaaaatga tcgcgaaatc agaacaggaa     3720

atcggtaagg ctaccgctaa atactttttt tattccaaca ttatgaattt ttttaagacc     3780

gaaataactc tcgcgaatgg tgaaatccgt aaacggcctc ttatagaaac caatggtgaa     3840

acgggagaaa tcgtttggga taaaggtcgt gactttgcca ccgttcgtaa agtcctctca     3900

atgccgcaag ttaacattgt caagaagacg gaagttcaaa cagggggatt ctccaaagaa     3960

tctatcctgc cgaagcgtaa cagtgataaa cttattgcca gaaaaaaaga ttgggatcca     4020

aaaaaatacg gaggctttga ttcccctacc gtcgcgtata gtgtgctggt ggttgctaaa     4080

gtcgagaaag ggaaaagcaa gaaattgaaa tcagttaaag aactgctggg tattacaatt     4140

atggaaagat cgtcctttga gaaaaatccg atcgactttt tagaggccaa ggggtataag     4200

gaagtgaaaa aagatctcat catcaaatta ccgaagtata gtctttttga gctggaaaac     4260

ggcagaaaaa gaatgctggc ctccgcgggc gagttacaga agggaaatga gctggcgctg     4320

ccttccaaat atgttaattt tctgtacctt gccagtcatt atgagaaact gaagggcagc     4380

cccgaagata acgaacagaa acaattattc gtggaacagc ataagcacta tttagatgaa     4440

attatagagc aaattagtga attttctaag cgcgttatcc tcgcggatgc taatttagac     4500

aaagtactgt cagcttataa taaacatcgg gataagccga ttagagaaca ggccgaaaat     4560

atcattcatt tgtttacctt aaccaacctt ggagcaccag ctgccttcaa atatttcgat     4620

accacaattg atcgtaaacg gtatacaagt acaaaagaag tcttggacgc aaccctcatt     4680

catcaatcta ttactggatt atatgagaca cgcattgatc tttcacagct gggcggagac     4740

aagaagaaaa aactgaaact gcaccatcat caccatcatc atcaccatca ttgataactc     4800

gagaaagctt acataaaaaa ccggccttgg ccccgccggt tttttattat ttttcttcct     4860

ccgcatgttc aatccgctcc ataatcgacg gatggctccc tctgaaaatt ttaacgagaa     4920

acggcgggtt gacccggctc agtcccgtaa cggccaagtc ctgaaacgtc tcaatcgccg     4980

cttcccggtt tccggtcagc tcaatgccgt aacggtcggc ggcgttttcc tgataccggg     5040

agacggcatt cgtaatcgaa ttcgcggccg cacgcgtcca tggggatccc cgcgggtcga     5100

cctcgagagt tacgctaggg ataacagggt aatataggag ctccagtcgg cttaaaccag     5160

ttttcgctgg tgcgaaaaaa gagtgtcttg tgacacctaa attcaaaatc tatcggtcag     5220

atttataccg atttgatttt atatattctt gaataacata cgccgagtta tcacataaaa     5280

gcgggaacca atcataaaat ttaaacttca ttgcataatc cattaaactc ttaaattcta     5340

cgattccttg ttcatcaata aactcaatca tttctttaat taatttatat ctatctgttg     5400

ttgttttctt taataattca ttaacatcta caccgccata aactatcata tcttcttttt     5460

gatatttaaa tttattagga tcgtccatgt gaagcatata tctcacaaga cctttcacac     5520

ttcctgcaat ctgcggaata gtcgcattca attcttctgt taattatttt tatctgttca     5580

taagatttat taccctcata catcactaga atatgataat gctctttttt catcctacct     5640

tctgtatcag tatccctatc atgtaatgga gacactacaa attgaatgtg taactctttt     5700

aaatactcta accactcggc ttttgctgat tctggatata aaacaaatgt ccaattacgt     5760

cctcttgaat ttttcttgtt ttcagtttct tttattacat tttcgctcat gatataataa     5820

cggtgctaat acacttaaca aaatttagtc atagataggc agcatgccag tgctgtctat     5880

ctttttttgt ttaaaatgca ccgtattcct cctttgcata tttttttatt agaataccgg     5940

ttgcatctga tttgctaata ttatattttt ctttgattct atttaatatc tcattttctt     6000

ctgttgtaag tcttaaagta acagcaactt ttttctcttc ttttctatct acaactatca     6060

ctgtacctcc caacatctgt ttttttcact ttaacataaa aaacaacctt ttaacattaa     6120

aaacccaata tttatttatt tgtttggaca atggacactg gacacctagg ggggaggtcg     6180

tagtaccccc ctatgttttc tcccctaaat aaccccaaaa atctaagaaa aaaagacctc     6240

aaaaaggtct ttaattaaca tctcaaattt cgcatttatt ccaatttcct ttttgcgtgt     6300

gatgcgagct catcggctcc gtcgatacta tgttatacgc caactttcaa aacaactttg     6360

aaaaagctgt tttctggtat ttaaggtttt agaatgcaag gaacagtgaa ttggagttcg     6420

tcttgttata attagcttct tggggtatct ttaaatactg tagaaaagag gaaggaaata     6480

ataaatggct aaaatgagaa tatcaccgga attgaaaaaa ctgatcgaaa aataccgctg     6540

cgtaaaagat acggaaggaa tgtctcctgc taaggtatat aagctggtgg gagaaaatga     6600

aaacctatat ttaaaaatga cggacagccg gtataaaggg accacctatg atgtggaacg     6660

ggaaaaggac atgatgctat ggctggaagg aaagctgcct gttccaaagg tcctgcactt     6720

tgaacggcat gatggctgga gcaatctgct catgagtgag gccgatggcg tcctttgctc     6780

ggaagagtat gaagatgaac aaagccctga aaagattatc gagctgtatg cggagtgcat     6840

caggctcttt cactccatcg acatatcgga ttgtccctat acgaatagct tagacagccg     6900

cttagccgaa ttggattact tactgaataa cgatctggcc gatgtggatt gcgaaaactg     6960

ggaagaagac actccattta aagatccgcg cgagctgtat gattttttaa agacggaaaa     7020

gcccgaagag gaacttgtct tttcccacgg cgacctggga gacagcaaca tctttgtgaa     7080

agatggcaaa gtaagtggct ttattgatct tgggagaagc ggcagggcgg acaagtggta     7140

tgacattgcc ttctgcgtcc ggtcgatcag ggaggatatc ggggaagaac agtatgtcga     7200

gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat attatatttt     7260

actggatgaa ttgttttagt gactgcagtg agatctggta atgactctct agcttgaggc     7320

atcaaataaa acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt     7380

cggtgaacgc tctcctgagt aggacaaatc cgccgctcta gctaagcaga aggccatcct     7440

gacggatggc ctttttgcgt ttctacaaac tcttgttaac tctagagctg cctgccgcgt     7500

ttcggtgatg aagatcttcc cgatgattaa ttaattcaga acgctcggtt gccgccgggc     7560

gttttttatg aagcttcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat     7620

cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag     7680

gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga     7740

tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg     7800

tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt     7860

cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac     7920

gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc     7980

ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt     8040

ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc     8100

ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc     8160

agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg     8220

aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag     8280

atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg     8340

tctgaca                                                               8347


<210>  22
<211>  846
<212>  DNA
<213>  Bacillus licheniformis

<400>  22
gtgaacagat cctttaccgt tgaaaaggta ctcaacaaca acgttttaat cgctctccat       60

gatgattaca gagaagttgt cttgattggc aaaggaatcg gttttggtaa aaagcgcgga      120

gatcttatcg aacatgagaa ctacgaaaaa atgtttatcc tggaaaatga taaggaacaa      180

tcgcagtata agaagctcct cacttatgtc gatgaaaaaa tggttgatat cgccaatgat      240

gtcatctacc atatcgcgca aaaaatcggc cagccgctga acgaacacat tcatgtcgcc      300

ctgacggacc atatcgcatt tgcagttaag cgtctagaaa agggatttga tatgaaaaat      360

ccgtttttgc ttgagacgga atcgctttat ccgaaggaat acgaagtcgc caaggaagcc      420

gtcgatatga ttaatgaaaa atccgacatt cagctgcctg aaggtgaaat cgggttcatc      480

gcgcttcata tccacagtgc gatgacaaac cgcccgcttt ctgaagtcaa tcagcattca      540

caactgatct ccaggcttgt ccaggtcatc gaagattcat tccagatgca agtcaacagg      600

gaaagcgtga actatttgcg gctgatcagg cacttgcgct ttacgattga caggataaaa      660

cgggacgagc cgattcagga accggaaaaa ttaatgttgt tgttgaaaac ggaatatccg      720

ctgtgttaca atactgcttg gaagatgatc aagatcttgc agcaagcgct caagaaaccg      780

gttcatgagg cagaagccgt ttatttgaca ttgcatttgt accgtttgac taataaaatt      840

tcataa                                                                 846


<210>  23
<211>  23
<212>  DNA
<213>  Bacillus licheniformis

<400>  23
ttttttcatc gacataagtg agg                                               23


<210>  24
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT VT domain

<400>  24
ttttttcatc gacataagtg                                                   20


<210>  25
<211>  3
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT target site PAM sequence

<400>  25
agg                                                                      3


<210>  26
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cas9 endonuclease recognition domain

<400>  26
gttttagagc tagaaatagc aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt       60

ggcaccgagt cggtgc                                                       76


<210>  27
<211>  96
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  glcT RNA

<400>  27
uuuuuucauc gacauaagug guuuuagagc uagaaauagc aaguuaaaau aaggcuaguc       60

cguuaucaac uugaaaaagu ggcaccgagu cggugc                                 96


<210>  28
<211>  96
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  DNA encoding glcT gRNA

<400>  28
ttttttcatc gacataagtg gttttagagc tagaaatagc aagttaaaat aaggctagtc       60

cgttatcaac ttgaaaaagt ggcaccgagt cggtgc                                 96


<210>  29
<211>  93
<212>  DNA
<213>  Bacillus subtilis

<400>  29
tcgctgataa acagctgaca tcaactaaaa gcttcattaa atactttgaa aaaagttgtt       60

gacttaaaag aagctaaatg ttatagtaat aaa                                    93


<210>  30
<211>  95
<212>  DNA
<213>  lambda phage

<400>  30
gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc       60

tcggttgccg ccgggcgttt tttattggtg agaat                                  95


<210>  31
<211>  284
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT gRNA expression cassette

<400>  31
tcgctgataa acagctgaca tcaactaaaa gcttcattaa atactttgaa aaaagttgtt       60

gacttaaaag aagctaaatg ttatagtaat aaattttttc atcgacataa gtggttttag      120

agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg      180

agtcggtgcg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt      240

tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaat                       284


<210>  32
<211>  500
<212>  DNA
<213>  Bacillus licheniformis

<400>  32
taagctgtga caaccagccg atcagcgaag tgctcggcat ttcaacaatt gaaatcaata       60

taaaggaaat ggggaagcgc gcgtttcgcc ttctgcaaaa aagaatcggc ggcgccgggc      120

cggaaaaact gactgttcct tacaagctga tcaaaagggc gactgtgtaa tcaaaacatc      180

aatctttttc cagacagtga tcaaaattcg acatttttca tcaaaaacgt caaaataaat      240

tgacgcgctt tcatgagttt tgtacgatat gagagattga tgacaaaaag gagctgagga      300

tcgtgaacag atcctttacc gttgaaaagg tactcaacaa caacgtttta atcgctctcc      360

atgatgatta cagagaagtt gtcttgattg gcaaaggaat cggttttggt aaaaagcgcg      420

gagatcttat cgaacatgag aactacgaaa aaatgtttat cctggaaaat gataaggaac      480

aatcgcagta taagaagctc                                                  500


<210>  33
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT 5' forward primer

<400>  33
tgagtaaact tggtctgaca taagctgtga caaccagc                               38


<210>  34
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT 5' reverse primer

<400>  34
ccattttttc atcgacataa gtgaagagct tcttatactg cgatt                       45


<210>  35
<211>  500
<212>  DNA
<213>  Bacillus licheniformis

<400>  35
tcacttatgt cgatgaaaaa atggttgata tcgccaatga tgtcatctac catatcgcgc       60

aaaaaatcgg ccagccgctg aacgaacaca ttcatgtcgc cctgacggac catatcgcat      120

ttgcagttaa gcgtctagaa aagggatttg atatgaaaaa tccgtttttg cttgagacgg      180

aatcgcttta tccgaaggaa tacgaagtcg ccaaggaagc cgtcgatatg attaatgaaa      240

aatccgacat tcagctgcct gaaggtgaaa tcgggttcat cgcgcttcat atccacagtg      300

cgatgacaaa ccgcccgctt tctgaagtca atcagcattc acaactgatc tccaggcttg      360

tccaggtcat cgaagattca ttccagatgc aagtcaacag ggaaagcgtg aactatttgc      420

ggctgatcag gcacttgcgc tttacgattg acaggataaa acgggacgag ccgattcagg      480

aaccggaaaa attaatgttg                                                  500


<210>  36
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT 3' forward primer

<400>  36
aatcgcagta taagaagctc ttcacttatg tcgatgaaaa aatgg                       45


<210>  37
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT 3' reverse primer

<400>  37
cagaagaaaa tggaggaatt ccaacattaa tttttccggt tcctga                      46


<210>  38
<211>  9632
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRF731

<400>  38
tcgctgataa acagctgaca tcaactaaaa gcttcattaa atactttgaa aaaagttgtt       60

gacttaaaag aagctaaatg ttatagtaat aaattttttc atcgacataa gtggttttag      120

agctagaaat agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg      180

agtcggtgcg actcctgttg atagatccag taatgacctc agaactccat ctggatttgt      240

tcagaacgct cggttgccgc cgggcgtttt ttattggtga gaatgaattc gcggccgcac      300

gcgtccatgg ggatccccgc gggtcgacct cgagagttac gctagggata acagggtaat      360

ataggagctc cagtcggctt aaaccagttt tcgctggtgc gaaaaaagag tgtcttgtga      420

cacctaaatt caaaatctat cggtcagatt tataccgatt tgattttata tattcttgaa      480

taacatacgc cgagttatca cataaaagcg ggaaccaatc ataaaattta aacttcattg      540

cataatccat taaactctta aattctacga ttccttgttc atcaataaac tcaatcattt      600

ctttaattaa tttatatcta tctgttgttg ttttctttaa taattcatta acatctacac      660

cgccataaac tatcatatct tctttttgat atttaaattt attaggatcg tccatgtgaa      720

gcatatatct cacaagacct ttcacacttc ctgcaatctg cggaatagtc gcattcaatt      780

cttctgttaa ttatttttat ctgttcataa gatttattac cctcatacat cactagaata      840

tgataatgct cttttttcat cctaccttct gtatcagtat ccctatcatg taatggagac      900

actacaaatt gaatgtgtaa ctcttttaaa tactctaacc actcggcttt tgctgattct      960

ggatataaaa caaatgtcca attacgtcct cttgaatttt tcttgttttc agtttctttt     1020

attacatttt cgctcatgat ataataacgg tgctaataca cttaacaaaa tttagtcata     1080

gataggcagc atgccagtgc tgtctatctt tttttgttta aaatgcaccg tattcctcct     1140

ttgcatattt ttttattaga ataccggttg catctgattt gctaatatta tatttttctt     1200

tgattctatt taatatctca ttttcttctg ttgtaagtct taaagtaaca gcaacttttt     1260

tctcttcttt tctatctaca actatcactg tacctcccaa catctgtttt tttcacttta     1320

acataaaaaa caacctttta acattaaaaa cccaatattt atttatttgt ttggacaatg     1380

gacactggac acctaggggg gaggtcgtag taccccccta tgttttctcc cctaaataac     1440

cccaaaaatc taagaaaaaa agacctcaaa aaggtcttta attaacatct caaatttcgc     1500

atttattcca atttcctttt tgcgtgtgat gcgagctcat cggctccgtc gatactatgt     1560

tatacgccaa ctttcaaaac aactttgaaa aagctgtttt ctggtattta aggttttaga     1620

atgcaaggaa cagtgaattg gagttcgtct tgttataatt agcttcttgg ggtatcttta     1680

aatactgtag aaaagaggaa ggaaataata aatggctaaa atgagaatat caccggaatt     1740

gaaaaaactg atcgaaaaat accgctgcgt aaaagatacg gaaggaatgt ctcctgctaa     1800

ggtatataag ctggtgggag aaaatgaaaa cctatattta aaaatgacgg acagccggta     1860

taaagggacc acctatgatg tggaacggga aaaggacatg atgctatggc tggaaggaaa     1920

gctgcctgtt ccaaaggtcc tgcactttga acggcatgat ggctggagca atctgctcat     1980

gagtgaggcc gatggcgtcc tttgctcgga agagtatgaa gatgaacaaa gccctgaaaa     2040

gattatcgag ctgtatgcgg agtgcatcag gctctttcac tccatcgaca tatcggattg     2100

tccctatacg aatagcttag acagccgctt agccgaattg gattacttac tgaataacga     2160

tctggccgat gtggattgcg aaaactggga agaagacact ccatttaaag atccgcgcga     2220

gctgtatgat tttttaaaga cggaaaagcc cgaagaggaa cttgtctttt cccacggcga     2280

cctgggagac agcaacatct ttgtgaaaga tggcaaagta agtggcttta ttgatcttgg     2340

gagaagcggc agggcggaca agtggtatga cattgccttc tgcgtccggt cgatcaggga     2400

ggatatcggg gaagaacagt atgtcgagct attttttgac ttactgggga tcaagcctga     2460

ttgggagaaa ataaaatatt atattttact ggatgaattg ttttagtgac tgcagtgaga     2520

tctggtaatg actctctagc ttgaggcatc aaataaaacg aaaggctcag tcgaaagact     2580

gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct cctgagtagg acaaatccgc     2640

cgctctagct aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct     2700

tgttaactct agagctgcct gccgcgtttc ggtgatgaag atcttcccga tgattaatta     2760

attcagaacg ctcggttgcc gccgggcgtt ttttatgaag cttcgttgct ggcgtttttc     2820

cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga     2880

aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct     2940

cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg     3000

gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag     3060

ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat     3120

cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac     3180

aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac     3240

tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc     3300

ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt     3360

tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc     3420

ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg     3480

agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca     3540

atctaaagta tatatgagta aacttggtct gacataagct gtgacaacca gccgatcagc     3600

gaagtgctcg gcatttcaac aattgaaatc aatataaagg aaatggggaa gcgcgcgttt     3660

cgccttctgc aaaaaagaat cggcggcgcc gggccggaaa aactgactgt tccttacaag     3720

ctgatcaaaa gggcgactgt gtaatcaaaa catcaatctt tttccagaca gtgatcaaaa     3780

ttcgacattt ttcatcaaaa acgtcaaaat aaattgacgc gctttcatga gttttgtacg     3840

atatgagaga ttgatgacaa aaaggagctg aggatcgtga acagatcctt taccgttgaa     3900

aaggtactca acaacaacgt tttaatcgct ctccatgatg attacagaga agttgtcttg     3960

attggcaaag gaatcggttt tggtaaaaag cgcggagatc ttatcgaaca tgagaactac     4020

gaaaaaatgt ttatcctgga aaatgataag gaacaatcgc agtataagaa gctcttcact     4080

tatgtcgatg aaaaaatggt tgatatcgcc aatgatgtca tctaccatat cgcgcaaaaa     4140

atcggccagc cgctgaacga acacattcat gtcgccctga cggaccatat cgcatttgca     4200

gttaagcgtc tagaaaaggg atttgatatg aaaaatccgt ttttgcttga gacggaatcg     4260

ctttatccga aggaatacga agtcgccaag gaagccgtcg atatgattaa tgaaaaatcc     4320

gacattcagc tgcctgaagg tgaaatcggg ttcatcgcgc ttcatatcca cagtgcgatg     4380

acaaaccgcc cgctttctga agtcaatcag cattcacaac tgatctccag gcttgtccag     4440

gtcatcgaag attcattcca gatgcaagtc aacagggaaa gcgtgaacta tttgcggctg     4500

atcaggcact tgcgctttac gattgacagg ataaaacggg acgagccgat tcaggaaccg     4560

gaaaaattaa tgttggaatt cctccatttt cttctgctat caaaataaca gactcgtgat     4620

tttccaaacg agctttcaaa aaagcctctg ccccttgcaa atcggatgcc tgtctataaa     4680

attcccgata ttggttaaac agcggcgcaa tggcggccgc atctgatgtc tttgcttggc     4740

gaatgttcat cttatttctt cctccctctc aataattttt tcattctatc ccttttctgt     4800

aaagtttatt tttcagaata cttttatcat catgctttga aaaaatatca cgataatatc     4860

cattgttctc acggaagcac acgcaggtca tttgaacgaa ttttttcgac aggaatttgc     4920

cgggactcag gagcatttaa cctaaaaaag catgacattt cagcataatg aacatttact     4980

catgtctatt ttcgttcttt tctgtatgaa aatagttatt tcgagtctct acggaaatag     5040

cgagagatga tatacctaaa tagagataaa atcatctcaa aaaaatgggt ctactaaaat     5100

attattccat ctattacaat aaattcacag aatagtcttt taagtaagtc tactctgaat     5160

ttttttaaaa ggagagggta actagtggcc ccaaaaaaga aacgcaaggt tatggataaa     5220

aaatacagca ttggtctgga tatcggaacc aacagcgttg ggtgggcagt aataacagat     5280

gaatacaaag tgccgtcaaa aaaatttaag gttctgggga atacagatcg ccacagcata     5340

aaaaagaatc tgattggggc attgctgttt gattcgggtg agacagctga ggccacgcgt     5400

ctgaaacgta cagcaagaag acgttacaca cgtcgtaaaa atcgtatttg ctacttacag     5460

gaaatttttt ctaacgaaat ggccaaggta gatgatagtt tcttccatcg tctcgaagaa     5520

tcttttctgg ttgaggaaga taaaaaacac gaacgtcacc ctatctttgg caatatcgtg     5580

gatgaagtgg cctatcatga aaaataccct acgatttatc atcttcgcaa gaagttggtt     5640

gatagtacgg acaaagcgga tctgcgttta atctatcttg cgttagcgca catgatcaaa     5700

tttcgtggtc atttcttaat tgaaggtgat ctgaatcctg ataactctga tgtggacaaa     5760

ttgtttatac aattagtgca aacctataat cagctgttcg aggaaaaccc cattaatgcc     5820

tctggagttg atgccaaagc gattttaagc gcgagacttt ctaagtcccg gcgtctggag     5880

aatctgatcg cccagttacc aggggaaaag aaaaatggtc tgtttggtaa tctgattgcc     5940

ctcagtctgg ggcttacccc gaacttcaaa tccaattttg acctggctga ggacgcaaag     6000

ctgcagctga gcaaagatac ttatgatgat gacctcgaca atctgctcgc ccagattggt     6060

gaccaatatg cggatctgtt tctggcagcg aagaatcttt cggatgctat cttgctgtcg     6120

gatattctgc gtgttaatac cgaaatcacc aaagcgcctc tgtctgcaag tatgatcaag     6180

agatacgacg agcaccacca ggacctgact cttcttaagg cactggtacg ccaacagctt     6240

ccggagaaat acaaagaaat attcttcgac cagtccaaga atggttacgc gggctacatc     6300

gatggtggtg catcacagga agagttctat aaatttatta aaccaatcct tgagaaaatg     6360

gatggcacgg aagagttact tgttaaactt aaccgcgaag acttgcttag aaagcaacgt     6420

acattcgaca acggctccat cccacaccag attcatttag gtgaacttca cgccatcttg     6480

cgcagacaag aagatttcta tcccttctta aaagacaatc gggagaaaat cgagaagatc     6540

ctgacgttcc gcattcccta ttatgtcggt cccctggcac gtggtaattc tcggtttgcc     6600

tggatgacgc gcaaaagtga ggaaaccatc accccttgga actttgaaga agtcgtggat     6660

aaaggtgcta gcgcgcagtc ttttatagaa agaatgacga acttcgataa aaacttgccc     6720

aacgaaaaag tcctgcccaa gcactctctt ttatatgagt actttactgt gtacaacgaa     6780

ctgactaaag tgaaatacgt tacggaaggt atgcgcaaac ctgcctttct tagtggcgag     6840

cagaaaaaag caattgtcga tcttctcttt aaaacgaatc gcaaggtaac tgtaaaacag     6900

ctgaaggaag attatttcaa aaagatcgaa tgctttgatt ctgtcgagat ctcgggtgtc     6960

gaagatcgtt tcaacgcttc cttagggacc tatcatgatt tgctgaagat aataaaagac     7020

aaagactttc tcgacaatga agaaaatgaa gatattctgg aggatattgt tttgaccttg     7080

accttattcg aagatagaga gatgatcgag gagcgcttaa aaacctatgc ccacctgttt     7140

gatgacaaag tcatgaagca attaaagcgc cgcagatata cggggtgggg ccgcttgagc     7200

cgcaagttga ttaacggtat tagagacaag cagagcggaa aaactatcct ggatttcctc     7260

aaatctgacg gatttgcgaa ccgcaatttt atgcagctta tacatgatga ttcgcttaca     7320

ttcaaagagg atattcagaa ggctcaggtg tctgggcaag gtgattcact ccacgaacat     7380

atagcaaatt tggccggctc tcctgcgatt aagaagggga tcctgcaaac agttaaagtt     7440

gtggatgaac ttgtaaaagt aatgggccgc cacaagccgg agaatatcgt gatagaaatg     7500

gcgcgcgaga atcaaacgac acaaaaaggt caaaagaact caagagagag aatgaagcgc     7560

attgaggagg ggataaagga acttggatct caaattctga aagaacatcc agttgaaaac     7620

actcagctgc aaaatgaaaa attgtacctg tactacctgc agaatggaag agacatgtac     7680

gtggatcagg aattggatat caatagactc tcggactatg acgtagatca cattgtccct     7740

cagagcttcc tcaaggatga ttctatagat aataaagtac ttacgagatc ggacaaaaat     7800

cgcggtaaat cggataacgt cccatcggag gaagtcgtta aaaagatgaa aaactattgg     7860

cgtcaactgc tgaacgccaa gctgatcaca cagcgtaagt ttgataatct gactaaagcc     7920

gaacgcggtg gtcttagtga actcgataaa gcaggattta taaaacggca gttagtagaa     7980

acgcgccaaa ttacgaaaca cgtggctcag atcctcgatt ctagaatgaa tacaaagtac     8040

gatgaaaacg ataaactgat ccgtgaagta aaagtcatta ccttaaaatc taaacttgtg     8100

tccgatttcc gcaaagattt tcagttttac aaggtccggg aaatcaataa ctatcaccat     8160

gcacatgatg catatttaaa tgcggttgta ggcacggccc ttattaagaa ataccctaaa     8220

ctcgaaagtg agtttgttta tggggattat aaagtgtatg acgttcgcaa aatgatcgcg     8280

aaatcagaac aggaaatcgg taaggctacc gctaaatact ttttttattc caacattatg     8340

aattttttta agaccgaaat aactctcgcg aatggtgaaa tccgtaaacg gcctcttata     8400

gaaaccaatg gtgaaacggg agaaatcgtt tgggataaag gtcgtgactt tgccaccgtt     8460

cgtaaagtcc tctcaatgcc gcaagttaac attgtcaaga agacggaagt tcaaacaggg     8520

ggattctcca aagaatctat cctgccgaag cgtaacagtg ataaacttat tgccagaaaa     8580

aaagattggg atccaaaaaa atacggaggc tttgattccc ctaccgtcgc gtatagtgtg     8640

ctggtggttg ctaaagtcga gaaagggaaa agcaagaaat tgaaatcagt taaagaactg     8700

ctgggtatta caattatgga aagatcgtcc tttgagaaaa atccgatcga ctttttagag     8760

gccaaggggt ataaggaagt gaaaaaagat ctcatcatca aattaccgaa gtatagtctt     8820

tttgagctgg aaaacggcag aaaaagaatg ctggcctccg cgggcgagtt acagaaggga     8880

aatgagctgg cgctgccttc caaatatgtt aattttctgt accttgccag tcattatgag     8940

aaactgaagg gcagccccga agataacgaa cagaaacaat tattcgtgga acagcataag     9000

cactatttag atgaaattat agagcaaatt agtgaatttt ctaagcgcgt tatcctcgcg     9060

gatgctaatt tagacaaagt actgtcagct tataataaac atcgggataa gccgattaga     9120

gaacaggccg aaaatatcat tcatttgttt accttaacca accttggagc accagctgcc     9180

ttcaaatatt tcgataccac aattgatcgt aaacggtata caagtacaaa agaagtcttg     9240

gacgcaaccc tcattcatca atctattact ggattatatg agacacgcat tgatctttca     9300

cagctgggcg gagacaagaa gaaaaaactg aaactgcacc atcatcacca tcatcatcac     9360

catcattgat aactcgagaa agcttacata aaaaaccggc cttggccccg ccggtttttt     9420

attatttttc ttcctccgca tgttcaatcc gctccataat cgacggatgg ctccctctga     9480

aaattttaac gagaaacggc gggttgaccc ggctcagtcc cgtaacggcc aagtcctgaa     9540

acgtctcaat cgccgcttcc cggtttccgg tcagctcaat gccgtaacgg tcggcggcgt     9600

tttcctgata ccgggagacg gcattcgtaa tc                                   9632


<210>  39
<211>  9632
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRF724

<400>  39
gaattcctcc attttcttct gctatcaaaa taacagactc gtgattttcc aaacgagctt       60

tcaaaaaagc ctctgcccct tgcaaatcgg atgcctgtct ataaaattcc cgatattggt      120

taaacagcgg cgcaatggcg gccgcatctg atgtctttgc ttggcgaatg ttcatcttat      180

ttcttcctcc ctctcaataa ttttttcatt ctatcccttt tctgtaaagt ttatttttca      240

gaatactttt atcatcatgc tttgaaaaaa tatcacgata atatccattg ttctcacgga      300

agcacacgca ggtcatttga acgaattttt tcgacaggaa tttgccggga ctcaggagca      360

tttaacctaa aaaagcatga catttcagca taatgaacat ttactcatgt ctattttcgt      420

tcttttctgt atgaaaatag ttatttcgag tctctacgga aatagcgaga gatgatatac      480

ctaaatagag ataaaatcat ctcaaaaaaa tgggtctact aaaatattat tccatctatt      540

acaataaatt cacagaatag tcttttaagt aagtctactc tgaatttttt taaaaggaga      600

gggtaactag tggccccaaa aaagaaacgc aaggttatgg ataaaaaata cagcattggt      660

ctggatatcg gaaccaacag cgttgggtgg gcagtaataa cagatgaata caaagtgccg      720

tcaaaaaaat ttaaggttct ggggaataca gatcgccaca gcataaaaaa gaatctgatt      780

ggggcattgc tgtttgattc gggtgagaca gctgaggcca cgcgtctgaa acgtacagca      840

agaagacgtt acacacgtcg taaaaatcgt atttgctact tacaggaaat tttttctaac      900

gaaatggcca aggtagatga tagtttcttc catcgtctcg aagaatcttt tctggttgag      960

gaagataaaa aacacgaacg tcaccctatc tttggcaata tcgtggatga agtggcctat     1020

catgaaaaat accctacgat ttatcatctt cgcaagaagt tggttgatag tacggacaaa     1080

gcggatctgc gtttaatcta tcttgcgtta gcgcacatga tcaaatttcg tggtcatttc     1140

ttaattgaag gtgatctgaa tcctgataac tctgatgtgg acaaattgtt tatacaatta     1200

gtgcaaacct ataatcagct gttcgaggaa aaccccatta atgcctctgg agttgatgcc     1260

aaagcgattt taagcgcgag actttctaag tcccggcgtc tggagaatct gatcgcccag     1320

ttaccagggg aaaagaaaaa tggtctgttt ggtaatctga ttgccctcag tctggggctt     1380

accccgaact tcaaatccaa ttttgacctg gctgaggacg caaagctgca gctgagcaaa     1440

gatacttatg atgatgacct cgacaatctg ctcgcccaga ttggtgacca atatgcggat     1500

ctgtttctgg cagcgaagaa tctttcggat gctatcttgc tgtcggatat tctgcgtgtt     1560

aataccgaaa tcaccaaagc gcctctgtct gcaagtatga tcaagagata cgacgagcac     1620

caccaggacc tgactcttct taaggcactg gtacgccaac agcttccgga gaaatacaaa     1680

gaaatattct tcgaccagtc caagaatggt tacgcgggct acatcgatgg tggtgcatca     1740

caggaagagt tctataaatt tattaaacca atccttgaga aaatggatgg cacggaagag     1800

ttacttgtta aacttaaccg cgaagacttg cttagaaagc aacgtacatt cgacaacggc     1860

tccatcccac accagattca tttaggtgaa cttcacgcca tcttgcgcag acaagaagat     1920

ttctatccct tcttaaaaga caatcgggag aaaatcgaga agatcctgac gttccgcatt     1980

ccctattatg tcggtcccct ggcacgtggt aattctcggt ttgcctggat gacgcgcaaa     2040

agtgaggaaa ccatcacccc ttggaacttt gaagaagtcg tggataaagg tgctagcgcg     2100

cagtctttta tagaaagaat gacgaacttc gataaaaact tgcccaacga aaaagtcctg     2160

cccaagcact ctcttttata tgagtacttt actgtgtaca acgaactgac taaagtgaaa     2220

tacgttacgg aaggtatgcg caaacctgcc tttcttagtg gcgagcagaa aaaagcaatt     2280

gtcgatcttc tctttaaaac gaatcgcaag gtaactgtaa aacagctgaa ggaagattat     2340

ttcaaaaaga tcgaatgctt tgattctgtc gagatctcgg gtgtcgaaga tcgtttcaac     2400

gcttccttag ggacctatca tgatttgctg aagataataa aagacaaaga ctttctcgac     2460

aatgaagaaa atgaagatat tctggaggat attgttttga ccttgacctt attcgaagat     2520

agagagatga tcgaggagcg cttaaaaacc tatgcccacc tgtttgatga caaagtcatg     2580

aagcaattaa agcgccgcag atatacgggg tggggccgct tgagccgcaa gttgattaac     2640

ggtattagag acaagcagag cggaaaaact atcctggatt tcctcaaatc tgacggattt     2700

gcgaaccgca attttatgca gcttatacat gatgattcgc ttacattcaa agaggatatt     2760

cagaaggctc aggtgtctgg gcaaggtgat tcactccacg aacatatagc aaatttggcc     2820

ggctctcctg cgattaagaa ggggatcctg caaacagtta aagttgtgga tgaacttgta     2880

aaagtaatgg gccgccacaa gccggagaat atcgtgatag aaatggcgcg cgagaatcaa     2940

acgacacaaa aaggtcaaaa gaactcaaga gagagaatga agcgcattga ggaggggata     3000

aaggaacttg gatctcaaat tctgaaagaa catccagttg aaaacactca gctgcaaaat     3060

gaaaaattgt acctgtacta cctgcagaat ggaagagaca tgtacgtgga tcaggaattg     3120

gatatcaata gactctcgga ctatgacgta gatcacattg tccctcagag cttcctcaag     3180

gatgattcta tagataataa agtacttacg agatcggaca aaaatcgcgg taaatcggat     3240

aacgtcccat cggaggaagt cgttaaaaag atgaaaaact attggcgtca actgctgaac     3300

gccaagctga tcacacagcg taagtttgat aatctgacta aagccgaacg cggtggtctt     3360

agtgaactcg ataaagcagg atttataaaa cggcagttag tagaaacgcg ccaaattacg     3420

aaacacgtgg ctcagatcct cgattctaga atgaatacaa agtacgatga aaacgataaa     3480

ctgatccgtg aagtaaaagt cattacctta aaatctaaac ttgtgtccga tttccgcaaa     3540

gattttcagt tttacaaggt ccgggaaatc aataactatc accatgcaca tgatgcatat     3600

ttaaatgcgg ttgtaggcac ggcccttatt aagaaatacc ctaaactcga aagtgagttt     3660

gtttatgggg attataaagt gtatgacgtt cgcaaaatga tcgcgaaatc agaacaggaa     3720

atcggtaagg ctaccgctaa atactttttt tattccaaca ttatgaattt ttttaagacc     3780

gaaataactc tcgcgaatgg tgaaatccgt aaacggcctc ttatagaaac caatggtgaa     3840

acgggagaaa tcgtttggga taaaggtcgt gactttgcca ccgttcgtaa agtcctctca     3900

atgccgcaag ttaacattgt caagaagacg gaagttcaaa cagggggatt ctccaaagaa     3960

tctatcctgc cgaagcgtaa cagtgataaa cttattgcca gaaaaaaaga ttgggatcca     4020

aaaaaatacg gaggctttga ttcccctacc gtcgcgtata gtgtgctggt ggttgctaaa     4080

gtcgagaaag ggaaaagcaa gaaattgaaa tcagttaaag aactgctggg tattacaatt     4140

atggaaagat cgtcctttga gaaaaatccg atcgactttt tagaggccaa ggggtataag     4200

gaagtgaaaa aagatctcat catcaaatta ccgaagtata gtctttttga gctggaaaac     4260

ggcagaaaaa gaatgctggc ctccgcgggc gagttacaga agggaaatga gctggcgctg     4320

ccttccaaat atgttaattt tctgtacctt gccagtcatt atgagaaact gaagggcagc     4380

cccgaagata acgaacagaa acaattattc gtggaacagc ataagcacta tttagatgaa     4440

attatagagc aaattagtga attttctaag cgcgttatcc tcgcggatgc taatttagac     4500

aaagtactgt cagcttataa taaacatcgg gataagccga ttagagaaca ggccgaaaat     4560

atcattcatt tgtttacctt aaccaacctt ggagcaccag ctgccttcaa atatttcgat     4620

accacaattg atcgtaaacg gtatacaagt acaaaagaag tcttggacgc aaccctcatt     4680

catcaatcta ttactggatt atatgagaca cgcattgatc tttcacagct gggcggagac     4740

aagaagaaaa aactgaaact gcaccatcat caccatcatc atcaccatca ttgataactc     4800

gagaaagctt acataaaaaa ccggccttgg ccccgccggt tttttattat ttttcttcct     4860

ccgcatgttc aatccgctcc ataatcgacg gatggctccc tctgaaaatt ttaacgagaa     4920

acggcgggtt gacccggctc agtcccgtaa cggccaagtc ctgaaacgtc tcaatcgccg     4980

cttcccggtt tccggtcagc tcaatgccgt aacggtcggc ggcgttttcc tgataccggg     5040

agacggcatt cgtaatcgta tgaggaactg atggcatctg caggctatat cagcgcgtct     5100

acagtccagg aagcaagaag cagctatgat tccatttacg acatcgtgtc acagtacgat     5160

ttagaggacc tttctctgtt tgacagcgaa aagtggaagg tgctttcaaa aaaagacatc     5220

gaaaacctgg acaaatattt cgactttctc gtgcaggaag caagcagccg aaacaaaaac     5280

tgaatacttc tccgcggcac actctcctct ctatcatttt cgtctgttta cgatcctgct     5340

gttattttat cccttatgtt aacttttgtc aatatttttc ctgtctaagt atttcctata     5400

gtcaacattt gtattaaaat gttcatatca tgaatttgcg ggggggatgg cgatgacaag     5460

gttcggcgag cggctcaaag agctgaggga acaaagaagc ctgtcggtta atcagcttgc     5520

catgtatgcc ggtgtgagcg ccgcagccat ttccagaatc gaaaacggcc accgcggcgt     5580

tcccaagccc gcgacgatca gaaaattggc cgaggctctg aaaatgccgt acgagcagct     5640

catggatatt gccggttata tgagagctga cgagattcgc gaacagccgc gcggctatgt     5700

cacgatgcag gagatcgcgg ccaagcacgg cgtcgaagac ctgtggctgt ttaaacccga     5760

gaaatgggac tgtttgtccc gcgaagacct gctcaacctc gaacagtatt ttcatttttt     5820

ggttaatgaa gcgaagaagc gccaatcata aaaagccgaa tttccctttt aggagaagtt     5880

cggctttttt cggctgcctt aagcggcatc cggattcggc gtcttgcctt tatgatgctt     5940

aacggggctc agcgcacgct cgagccatcc catgaacaga tcggcgatga tcgccatcag     6000

cgccgtcggg atcgcgcctg ctagaatgat cgctgttccg ttggtcgcgt ttgatcctcg     6060

ctgataaaca gctgacatca actaaaagct tcattaaata ctttgaaaaa agttgttgac     6120

ttaaaagaag ctaaatgtta tagtaataaa aaatggctgc ggctctggaa agttttagag     6180

ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag tggcaccgag     6240

tcggtgcgac tcctgttgat agatccagta atgacctcag aactccatct ggatttgttc     6300

agaacgctcg gttgccgccg ggcgtttttt attggtgaga atgaattcgc ggccgcacgc     6360

gtccatgggg atccccgcgg gtcgacctcg agagttacgc tagggataac agggtaatat     6420

aggagctcca gtcggcttaa accagttttc gctggtgcga aaaaagagtg tcttgtgaca     6480

cctaaattca aaatctatcg gtcagattta taccgatttg attttatata ttcttgaata     6540

acatacgccg agttatcaca taaaagcggg aaccaatcat aaaatttaaa cttcattgca     6600

taatccatta aactcttaaa ttctacgatt ccttgttcat caataaactc aatcatttct     6660

ttaattaatt tatatctatc tgttgttgtt ttctttaata attcattaac atctacaccg     6720

ccataaacta tcatatcttc tttttgatat ttaaatttat taggatcgtc catgtgaagc     6780

atatatctca caagaccttt cacacttcct gcaatctgcg gaatagtcgc attcaattct     6840

tctgttaatt atttttatct gttcataaga tttattaccc tcatacatca ctagaatatg     6900

ataatgctct tttttcatcc taccttctgt atcagtatcc ctatcatgta atggagacac     6960

tacaaattga atgtgtaact cttttaaata ctctaaccac tcggcttttg ctgattctgg     7020

atataaaaca aatgtccaat tacgtcctct tgaatttttc ttgttttcag tttcttttat     7080

tacattttcg ctcatgatat aataacggtg ctaatacact taacaaaatt tagtcataga     7140

taggcagcat gccagtgctg tctatctttt tttgtttaaa atgcaccgta ttcctccttt     7200

gcatattttt ttattagaat accggttgca tctgatttgc taatattata tttttctttg     7260

attctattta atatctcatt ttcttctgtt gtaagtctta aagtaacagc aacttttttc     7320

tcttcttttc tatctacaac tatcactgta cctcccaaca tctgtttttt tcactttaac     7380

ataaaaaaca accttttaac attaaaaacc caatatttat ttatttgttt ggacaatgga     7440

cactggacac ctagggggga ggtcgtagta cccccctatg ttttctcccc taaataaccc     7500

caaaaatcta agaaaaaaag acctcaaaaa ggtctttaat taacatctca aatttcgcat     7560

ttattccaat ttcctttttg cgtgtgatgc gagctcatcg gctccgtcga tactatgtta     7620

tacgccaact ttcaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat     7680

gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa     7740

tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga     7800

aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg     7860

tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata     7920

aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc     7980

tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga     8040

gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga     8100

ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc     8160

cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc     8220

tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc     8280

tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc     8340

tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga     8400

gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg     8460

atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt     8520

gggagaaaat aaaatattat attttactgg atgaattgtt ttagtgactg cagtgagatc     8580

tggtaatgac tctctagctt gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg     8640

gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg     8700

ctctagctaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactcttg     8760

ttaactctag agctgcctgc cgcgtttcgg tgatgaagat cttcccgatg attaattaat     8820

tcagaacgct cggttgccgc cgggcgtttt ttatgaagct tcgttgctgg cgtttttcca     8880

taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa     8940

cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc     9000

tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc     9060

gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct     9120

gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg     9180

tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag     9240

gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta     9300

cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg     9360

aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt     9420

tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt     9480

ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag     9540

attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat     9600

ctaaagtata tatgagtaaa cttggtctga ca                                   9632


<210>  40
<211>  18
<212>  DNA
<213>  Bacillus licheniformis

<400>  40
gccgcagcca tttccaga                                                     18


<210>  41
<211>  417
<212>  DNA
<213>  Bacillus licheniformis

<400>  41
atgacaaggt tcggcgagcg gctcaaagag ctgagggaac aaagaagcct gtcggttaat       60

cagcttgcca tgtatgccgg tgtgagcgcc gcagccattt ccagagccgc agccatttcc      120

agaatcgaaa acggccaccg cggcgttccc aagcccgcga cgatcagaaa attggccgag      180

gctctgaaaa tgccgtacga gcagctcatg gatattgccg gttatatgag agctgacgag      240

attcgcgaac agccgcgcgg ctatgtcacg atgcaggaga tcgcggccaa gcacggcgtc      300

gaagacctgt ggctgtttaa acccgagaaa tgggactgtt tgtcccgcga agacctgctc      360

aacctcgaac agtattttca ttttttggtt aatgaagcga agaagcgcca atcataa         417


<210>  42
<211>  8347
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  8.3kb PCR of pRF694

<400>  42
gaattcgcgg ccgcacgcgt ccatggggat ccccgcgggt cgacctcgag agttacgcta       60

gggataacag ggtaatatag gagctccagt cggcttaaac cagttttcgc tggtgcgaaa      120

aaagagtgtc ttgtgacacc taaattcaaa atctatcggt cagatttata ccgatttgat      180

tttatatatt cttgaataac atacgccgag ttatcacata aaagcgggaa ccaatcataa      240

aatttaaact tcattgcata atccattaaa ctcttaaatt ctacgattcc ttgttcatca      300

ataaactcaa tcatttcttt aattaattta tatctatctg ttgttgtttt ctttaataat      360

tcattaacat ctacaccgcc ataaactatc atatcttctt tttgatattt aaatttatta      420

ggatcgtcca tgtgaagcat atatctcaca agacctttca cacttcctgc aatctgcgga      480

atagtcgcat tcaattcttc tgttaattat ttttatctgt tcataagatt tattaccctc      540

atacatcact agaatatgat aatgctcttt tttcatccta ccttctgtat cagtatccct      600

atcatgtaat ggagacacta caaattgaat gtgtaactct tttaaatact ctaaccactc      660

ggcttttgct gattctggat ataaaacaaa tgtccaatta cgtcctcttg aatttttctt      720

gttttcagtt tcttttatta cattttcgct catgatataa taacggtgct aatacactta      780

acaaaattta gtcatagata ggcagcatgc cagtgctgtc tatctttttt tgtttaaaat      840

gcaccgtatt cctcctttgc atattttttt attagaatac cggttgcatc tgatttgcta      900

atattatatt tttctttgat tctatttaat atctcatttt cttctgttgt aagtcttaaa      960

gtaacagcaa cttttttctc ttcttttcta tctacaacta tcactgtacc tcccaacatc     1020

tgtttttttc actttaacat aaaaaacaac cttttaacat taaaaaccca atatttattt     1080

atttgtttgg acaatggaca ctggacacct aggggggagg tcgtagtacc cccctatgtt     1140

ttctccccta aataacccca aaaatctaag aaaaaaagac ctcaaaaagg tctttaatta     1200

acatctcaaa tttcgcattt attccaattt cctttttgcg tgtgatgcga gctcatcggc     1260

tccgtcgata ctatgttata cgccaacttt caaaacaact ttgaaaaagc tgttttctgg     1320

tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt ataattagct     1380

tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg gctaaaatga     1440

gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa gatacggaag     1500

gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta tatttaaaaa     1560

tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag gacatgatgc     1620

tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg catgatggct     1680

ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag tatgaagatg     1740

aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc tttcactcca     1800

tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc gaattggatt     1860

acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa gacactccat     1920

ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa gaggaacttg     1980

tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc aaagtaagtg     2040

gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt gccttctgcg     2100

tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt tttgacttac     2160

tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat gaattgtttt     2220

agtgactgca gtgagatctg gtaatgactc tctagcttga ggcatcaaat aaaacgaaag     2280

gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg     2340

agtaggacaa atccgccgct ctagctaagc agaaggccat cctgacggat ggcctttttg     2400

cgtttctaca aactcttgtt aactctagag ctgcctgccg cgtttcggtg atgaagatct     2460

tcccgatgat taattaattc agaacgctcg gttgccgccg ggcgtttttt atgaagcttc     2520

gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc     2580

aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag     2640

ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct     2700

cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta     2760

ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc     2820

cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc     2880

agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt     2940

gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct gcgctctgct     3000

gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc     3060

tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca     3120

agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta     3180

agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa     3240

atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca gaattcctcc     3300

attttcttct gctatcaaaa taacagactc gtgattttcc aaacgagctt tcaaaaaagc     3360

ctctgcccct tgcaaatcgg atgcctgtct ataaaattcc cgatattggt taaacagcgg     3420

cgcaatggcg gccgcatctg atgtctttgc ttggcgaatg ttcatcttat ttcttcctcc     3480

ctctcaataa ttttttcatt ctatcccttt tctgtaaagt ttatttttca gaatactttt     3540

atcatcatgc tttgaaaaaa tatcacgata atatccattg ttctcacgga agcacacgca     3600

ggtcatttga acgaattttt tcgacaggaa tttgccggga ctcaggagca tttaacctaa     3660

aaaagcatga catttcagca taatgaacat ttactcatgt ctattttcgt tcttttctgt     3720

atgaaaatag ttatttcgag tctctacgga aatagcgaga gatgatatac ctaaatagag     3780

ataaaatcat ctcaaaaaaa tgggtctact aaaatattat tccatctatt acaataaatt     3840

cacagaatag tcttttaagt aagtctactc tgaatttttt taaaaggaga gggtaactag     3900

tggccccaaa aaagaaacgc aaggttatgg ataaaaaata cagcattggt ctggatatcg     3960

gaaccaacag cgttgggtgg gcagtaataa cagatgaata caaagtgccg tcaaaaaaat     4020

ttaaggttct ggggaataca gatcgccaca gcataaaaaa gaatctgatt ggggcattgc     4080

tgtttgattc gggtgagaca gctgaggcca cgcgtctgaa acgtacagca agaagacgtt     4140

acacacgtcg taaaaatcgt atttgctact tacaggaaat tttttctaac gaaatggcca     4200

aggtagatga tagtttcttc catcgtctcg aagaatcttt tctggttgag gaagataaaa     4260

aacacgaacg tcaccctatc tttggcaata tcgtggatga agtggcctat catgaaaaat     4320

accctacgat ttatcatctt cgcaagaagt tggttgatag tacggacaaa gcggatctgc     4380

gtttaatcta tcttgcgtta gcgcacatga tcaaatttcg tggtcatttc ttaattgaag     4440

gtgatctgaa tcctgataac tctgatgtgg acaaattgtt tatacaatta gtgcaaacct     4500

ataatcagct gttcgaggaa aaccccatta atgcctctgg agttgatgcc aaagcgattt     4560

taagcgcgag actttctaag tcccggcgtc tggagaatct gatcgcccag ttaccagggg     4620

aaaagaaaaa tggtctgttt ggtaatctga ttgccctcag tctggggctt accccgaact     4680

tcaaatccaa ttttgacctg gctgaggacg caaagctgca gctgagcaaa gatacttatg     4740

atgatgacct cgacaatctg ctcgcccaga ttggtgacca atatgcggat ctgtttctgg     4800

cagcgaagaa tctttcggat gctatcttgc tgtcggatat tctgcgtgtt aataccgaaa     4860

tcaccaaagc gcctctgtct gcaagtatga tcaagagata cgacgagcac caccaggacc     4920

tgactcttct taaggcactg gtacgccaac agcttccgga gaaatacaaa gaaatattct     4980

tcgaccagtc caagaatggt tacgcgggct acatcgatgg tggtgcatca caggaagagt     5040

tctataaatt tattaaacca atccttgaga aaatggatgg cacggaagag ttacttgtta     5100

aacttaaccg cgaagacttg cttagaaagc aacgtacatt cgacaacggc tccatcccac     5160

accagattca tttaggtgaa cttcacgcca tcttgcgcag acaagaagat ttctatccct     5220

tcttaaaaga caatcgggag aaaatcgaga agatcctgac gttccgcatt ccctattatg     5280

tcggtcccct ggcacgtggt aattctcggt ttgcctggat gacgcgcaaa agtgaggaaa     5340

ccatcacccc ttggaacttt gaagaagtcg tggataaagg tgctagcgcg cagtctttta     5400

tagaaagaat gacgaacttc gataaaaact tgcccaacga aaaagtcctg cccaagcact     5460

ctcttttata tgagtacttt actgtgtaca acgaactgac taaagtgaaa tacgttacgg     5520

aaggtatgcg caaacctgcc tttcttagtg gcgagcagaa aaaagcaatt gtcgatcttc     5580

tctttaaaac gaatcgcaag gtaactgtaa aacagctgaa ggaagattat ttcaaaaaga     5640

tcgaatgctt tgattctgtc gagatctcgg gtgtcgaaga tcgtttcaac gcttccttag     5700

ggacctatca tgatttgctg aagataataa aagacaaaga ctttctcgac aatgaagaaa     5760

atgaagatat tctggaggat attgttttga ccttgacctt attcgaagat agagagatga     5820

tcgaggagcg cttaaaaacc tatgcccacc tgtttgatga caaagtcatg aagcaattaa     5880

agcgccgcag atatacgggg tggggccgct tgagccgcaa gttgattaac ggtattagag     5940

acaagcagag cggaaaaact atcctggatt tcctcaaatc tgacggattt gcgaaccgca     6000

attttatgca gcttatacat gatgattcgc ttacattcaa agaggatatt cagaaggctc     6060

aggtgtctgg gcaaggtgat tcactccacg aacatatagc aaatttggcc ggctctcctg     6120

cgattaagaa ggggatcctg caaacagtta aagttgtgga tgaacttgta aaagtaatgg     6180

gccgccacaa gccggagaat atcgtgatag aaatggcgcg cgagaatcaa acgacacaaa     6240

aaggtcaaaa gaactcaaga gagagaatga agcgcattga ggaggggata aaggaacttg     6300

gatctcaaat tctgaaagaa catccagttg aaaacactca gctgcaaaat gaaaaattgt     6360

acctgtacta cctgcagaat ggaagagaca tgtacgtgga tcaggaattg gatatcaata     6420

gactctcgga ctatgacgta gatcacattg tccctcagag cttcctcaag gatgattcta     6480

tagataataa agtacttacg agatcggaca aaaatcgcgg taaatcggat aacgtcccat     6540

cggaggaagt cgttaaaaag atgaaaaact attggcgtca actgctgaac gccaagctga     6600

tcacacagcg taagtttgat aatctgacta aagccgaacg cggtggtctt agtgaactcg     6660

ataaagcagg atttataaaa cggcagttag tagaaacgcg ccaaattacg aaacacgtgg     6720

ctcagatcct cgattctaga atgaatacaa agtacgatga aaacgataaa ctgatccgtg     6780

aagtaaaagt cattacctta aaatctaaac ttgtgtccga tttccgcaaa gattttcagt     6840

tttacaaggt ccgggaaatc aataactatc accatgcaca tgatgcatat ttaaatgcgg     6900

ttgtaggcac ggcccttatt aagaaatacc ctaaactcga aagtgagttt gtttatgggg     6960

attataaagt gtatgacgtt cgcaaaatga tcgcgaaatc agaacaggaa atcggtaagg     7020

ctaccgctaa atactttttt tattccaaca ttatgaattt ttttaagacc gaaataactc     7080

tcgcgaatgg tgaaatccgt aaacggcctc ttatagaaac caatggtgaa acgggagaaa     7140

tcgtttggga taaaggtcgt gactttgcca ccgttcgtaa agtcctctca atgccgcaag     7200

ttaacattgt caagaagacg gaagttcaaa cagggggatt ctccaaagaa tctatcctgc     7260

cgaagcgtaa cagtgataaa cttattgcca gaaaaaaaga ttgggatcca aaaaaatacg     7320

gaggctttga ttcccctacc gtcgcgtata gtgtgctggt ggttgctaaa gtcgagaaag     7380

ggaaaagcaa gaaattgaaa tcagttaaag aactgctggg tattacaatt atggaaagat     7440

cgtcctttga gaaaaatccg atcgactttt tagaggccaa ggggtataag gaagtgaaaa     7500

aagatctcat catcaaatta ccgaagtata gtctttttga gctggaaaac ggcagaaaaa     7560

gaatgctggc ctccgcgggc gagttacaga agggaaatga gctggcgctg ccttccaaat     7620

atgttaattt tctgtacctt gccagtcatt atgagaaact gaagggcagc cccgaagata     7680

acgaacagaa acaattattc gtggaacagc ataagcacta tttagatgaa attatagagc     7740

aaattagtga attttctaag cgcgttatcc tcgcggatgc taatttagac aaagtactgt     7800

cagcttataa taaacatcgg gataagccga ttagagaaca ggccgaaaat atcattcatt     7860

tgtttacctt aaccaacctt ggagcaccag ctgccttcaa atatttcgat accacaattg     7920

atcgtaaacg gtatacaagt acaaaagaag tcttggacgc aaccctcatt catcaatcta     7980

ttactggatt atatgagaca cgcattgatc tttcacagct gggcggagac aagaagaaaa     8040

aactgaaact gcaccatcat caccatcatc atcaccatca ttgataactc gagaaagctt     8100

acataaaaaa ccggccttgg ccccgccggt tttttattat ttttcttcct ccgcatgttc     8160

aatccgctcc ataatcgacg gatggctccc tctgaaaatt ttaacgagaa acggcgggtt     8220

gacccggctc agtcccgtaa cggccaagtc ctgaaacgtc tcaatcgccg cttcccggtt     8280

tccggtcagc tcaatgccgt aacggtcggc ggcgttttcc tgataccggg agacggcatt     8340

cgtaatc                                                               8347


<210>  43
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRF694 forward

<400>  43
gaattcgcgg ccgcacg                                                      17


<210>  44
<211>  29
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pRF694 reverse

<400>  44
gattacgaat gccgtctccc ggtatcagg                                         29


<210>  45
<211>  1365
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 ET gRNA cassette

<400>  45
ggcggcgttt tcctgatacc gggagacggc attcgtaatc gtatgaggaa ctgatggcat       60

ctgcaggcta tatcagcgcg tctacagtcc aggaagcaag aagcagctat gattccattt      120

acgacatcgt gtcacagtac gatttagagg acctttctct gtttgacagc gaaaagtgga      180

aggtgctttc aaaaaaagac atcgaaaacc tggacaaata tttcgacttt ctcgtgcagg      240

aagcaagcag ccgaaacaaa aactgaatac ttctccgcgg cacactctcc tctctatcat      300

tttcgtctgt ttacgatcct gctgttattt tatcccttat gttaactttt gtcaatattt      360

ttcctgtcta agtatttcct atagtcaaca tttgtattaa aatgttcata tcatgaattt      420

gcggggggga tggcgatgac aaggttcggc gagcggctca aagagctgag ggaacaaaga      480

agcctgtcgg ttaatcagct tgccatgtat gccggtgtga gcgccgcagc catttccaga      540

atcgaaaacg gccaccgcgg cgttcccaag cccgcgacga tcagaaaatt ggccgaggct      600

ctgaaaatgc cgtacgagca gctcatggat attgccggtt atatgagagc tgacgagatt      660

cgcgaacagc cgcgcggcta tgtcacgatg caggagatcg cggccaagca cggcgtcgaa      720

gacctgtggc tgtttaaacc cgagaaatgg gactgtttgt cccgcgaaga cctgctcaac      780

ctcgaacagt attttcattt tttggttaat gaagcgaaga agcgccaatc ataaaaagcc      840

gaatttccct tttaggagaa gttcggcttt tttcggctgc cttaagcggc atccggattc      900

ggcgtcttgc ctttatgatg cttaacgggg ctcagcgcac gctcgagcca tcccatgaac      960

agatcggcga tgatcgccat cagcgccgtc gggatcgcgc ctgctagaat gatcgctgtt     1020

ccgttggtcg cgtttgatcc tcgctgataa acagctgaca tcaactaaaa gcttcattaa     1080

atactttgaa aaaagttgtt gacttaaaag aagctaaatg ttatagtaat aaaaaatggc     1140

tgcggctctg gaaagtttta gagctagaaa tagcaagtta aaataaggct agtccgttat     1200

caacttgaaa aagtggcacc gagtcggtgc gactcctgtt gatagatcca gtaatgacct     1260

cagaactcca tctggatttg ttcagaacgc tcggttgccg ccgggcgttt tttattggtg     1320

agaatgaatt cgcggccgca cgcgtccatg gggatccccg cgggt                     1365


<210>  46
<211>  1000
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 editing template

<400>  46
gtatgaggaa ctgatggcat ctgcaggcta tatcagcgcg tctacagtcc aggaagcaag       60

aagcagctat gattccattt acgacatcgt gtcacagtac gatttagagg acctttctct      120

gtttgacagc gaaaagtgga aggtgctttc aaaaaaagac atcgaaaacc tggacaaata      180

tttcgacttt ctcgtgcagg aagcaagcag ccgaaacaaa aactgaatac ttctccgcgg      240

cacactctcc tctctatcat tttcgtctgt ttacgatcct gctgttattt tatcccttat      300

gttaactttt gtcaatattt ttcctgtcta agtatttcct atagtcaaca tttgtattaa      360

aatgttcata tcatgaattt gcggggggga tggcgatgac aaggttcggc gagcggctca      420

aagagctgag ggaacaaaga agcctgtcgg ttaatcagct tgccatgtat gccggtgtga      480

gcgccgcagc catttccaga atcgaaaacg gccaccgcgg cgttcccaag cccgcgacga      540

tcagaaaatt ggccgaggct ctgaaaatgc cgtacgagca gctcatggat attgccggtt      600

atatgagagc tgacgagatt cgcgaacagc cgcgcggcta tgtcacgatg caggagatcg      660

cggccaagca cggcgtcgaa gacctgtggc tgtttaaacc cgagaaatgg gactgtttgt      720

cccgcgaaga cctgctcaac ctcgaacagt attttcattt tttggttaat gaagcgaaga      780

agcgccaatc ataaaaagcc gaatttccct tttaggagaa gttcggcttt tttcggctgc      840

cttaagcggc atccggattc ggcgtcttgc ctttatgatg cttaacgggg ctcagcgcac      900

gctcgagcca tcccatgaac agatcggcga tgatcgccat cagcgccgtc gggatcgcgc      960

ctgctagaat gatcgctgtt ccgttggtcg cgtttgatcc                           1000


<210>  47
<211>  285
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 gRNA expression cassette

<400>  47
tcgctgataa acagctgaca tcaactaaaa gcttcattaa atactttgaa aaaagttgtt       60

gacttaaaag aagctaaatg ttatagtaat aaaaaatggc tgcggctctg gaaagtttta      120

gagctagaaa tagcaagtta aaataaggct agtccgttat caacttgaaa aagtggcacc      180

gagtcggtgc gactcctgtt gatagatcca gtaatgacct cagaactcca tctggatttg      240

ttcagaacgc tcggttgccg ccgggcgttt tttattggtg agaat                      285


<210>  48
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 cassette forward primer

<400>  48
ggcggcgttt tcctgatacc gggag                                             25


<210>  49
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 cassette reverse primer

<400>  49
acccgcgggg atccccatgg                                                   20


<210>  50
<211>  6393
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  plasmid pBL.comK

<400>  50
aagcttcata tgcaagggtt tattgttttc taaaatctga ttaccaatta gaatgaatat       60

ttcccaaata ttaaataata aaacaaaaaa attgaaaaaa gtgtttccac cattttttca      120

atttttttat aattttttta atctgttatt taaatagttt atagttaaat ttacattttc      180

attagtccat tcaatattct ctccaagata actacgaact gctaacaaaa ttctctccct      240

atgttctaat ggagaagatt cagccactgc atttcccgca atatcttttg gtatgatttt      300

acccgtgtcc atagttaaaa tcatacggca taaagttaat atagagttgg tttcatcatc      360

ctgataatta tctattaatt cctctgacga atccataatg gctcttctca catcagaaaa      420

tggaatatca ggtagtaatt cctctaagtc ataatttccg tatattcttt tattttttcg      480

ttttgcttgg taaagcatta tggttaaatc tgaatttaat tccttctgag gaatgtatcc      540

ttgttcataa agctcttgta accattctcc ataaataaat tcttgtttgg gaggatgatt      600

ccacggtacc atttcttgct gaataataat tgttaattca atatatcgta agttgctttt      660

atctcctatt ttttttgaaa taggtctaat tttttgtata agtatttctt tactttgatc      720

tgtcaatggt tcagatacga cgactaaaaa gtcaagatca ctatttggtt ttagtccact      780

ctcaactcct gatccaaaca tgtaagtacc aataaggtta ttttttaaat gtttccgaag      840

tatttttttc actttattaa tttgttcgta tgtattcaaa tatatcctcc tcactatttt      900

gattagtacc tattttatat ccatagttgt taattaaata aacttaattt agtttattta      960

tggatttcat tggcttctaa attttttatc tagataataa ttattttagt taattttatt     1020

ctagattata tatgatatga tctttcattt ccataaaact aaagtaagtg taaacctatt     1080

cattgtttta aaaatatctc ttgccagtca cgttacgtta ttagttatag ttattataac     1140

atgtattcac gaacgggcgc gccggtatcc gcgcttcttg agcactattt attcaaagcc     1200

gctccagatc aatagcgctt tttcagctcc ctgaggatga attcgtatat cagctgattc     1260

cggtcttctt tcggatagag cataaattcc tgtttcttct gcatggggtt tccttcaatc     1320

ctgtcgataa attttgttct cagccatgcc gttcggtaaa cctggttttc gaaagatgag     1380

atggatacgg gcagctccag cgtttccccg ttgacaaacg tgacaaacgt gttgtcatac     1440

tttgccgcgc aaaactcgtg aacatgcgca tgggaaagcc acccgcactg aggacgagtt     1500

gaggaaaatg tggggaaaag aaaaatgttg tttgagtgat ccaccatgat cggcggttta     1560

tgggaaactt taatgacttc atatgtgccc gcttttcttc ccgcatagct cgatccgaaa     1620

tagcggcagc ttctttcgat aatttgaaac ggcttcatat tgacgcggaa agtcctgtcg     1680

gtctcaagta tttttgaggc ggatttctcc ccctcaccca gaggcaggac agccattgtc     1740

gaactgttta cttcatacgt atcctttgtc atatcctctg tgctcatgtg atttccccct     1800

taaaaataaa ttcattcaaa tacagatgca ttttatttca tatagtaagt acatcaccta     1860

ttagtttgtt gtttaaacaa actaacttat tttcatctta tataacctcg tcagtatttt     1920

caatattttt tttagttttt tatgaacaca ttagatttaa taaagggaag attcgctatg     1980

tactatgttg atacttaatt taaagattaa acaaatggag tggatgaagt ggatatcgct     2040

gatcaaacct ttgtcaaaaa agtaaatcaa aagttattat taaaagaaat ccttaaaaat     2100

tcacctattt caagagcaaa attatctgaa atgactggat taaataaatc aactgtctca     2160

tcacaggtaa acacgttaat gaaagaaagt atggtatttg aaataggtca aggacaatca     2220

agtggcggaa gaagacctgt catgcttgtt tttaataaaa aggcaggata ctccgttgga     2280

atagatgttg gtgtggatta tattaatggc attttaacag accttgaagg aacaatcgtt     2340

cttgatcaat accgccattt ggaatccaat tctccagaaa taacgaaaga cattttgatt     2400

gatatgattc atcactttat tacgcaaatg ccccaatctc cgtacgggtt tattggtata     2460

ggtacttgcg tgcctggact cattgataaa gatcaaaaaa ttgttttcac tccgaactcc     2520

aactggagag atattgactt aaaatcttcg atacaagaga agtacaatgt gtctgttttt     2580

attgaaaatg aggcaaatgc tggcgcatat ggagaaaaac tatttggagc tgcaaaaaat     2640

cacgataaca ttatttacgt aagtatcagc acaggaatag ggatcggtgt tattatcaac     2700

aatcatttat atagaggagt aagcggcttc tctggagaaa tgggacatat gacaatagac     2760

tttaatggtc ctaaatgcag ttgcggaaac cgaggatgct gggaattgta tgcttcagag     2820

aaggctttat ttaaatctct tcagaccaaa gagaaaaaac tgtcctatca agatatcata     2880

aacctcgccc atctgaatga tatcggaacc ttaaatgcat tacaaaattt tggattctat     2940

ttaggaatag gccttaccaa tattctaaat actctcaacc cacaagccgt aattttaaga     3000

aatagcataa ttgaatcgca tcctatggtt ttaaattcaa tgagaagtga agtatcatca     3060

agggtttatt cccaattagg caatagctat gaattattgc catcttcctt aggacagaat     3120

gcaccggcat taggaatgtc ctccattgtg attgatcatt ttctggacat gattacaatg     3180

taatttttta tggaatggac agctcatctt taaagatgag tttttttatt ctaggagtat     3240

ttctgaagca atagtgacat ggcaccttct catatgaaaa aggagttcta aaataaaaat     3300

ctcctttttc atgtgcaaat tatttttctt tataacgaaa atatctaaat gacaatgcat     3360

atgcaagagg ggatcacata aatatatatt ttaaaaatat cccactttat ccaattttcg     3420

tttgttgaac taatgggtgc tttagttgaa gaataaaaga ccacattaaa aaatgtggtc     3480

ttttgtgttt ttttaaagga tttgagcgta gcgaaaaatc cttttctttc ttatcttgat     3540

actatataga aacaacatca tttttcaaaa ttaggtcaaa gccttgtgta tcaagggttt     3600

gatggttctt tgacaggtaa aaactccttc tgctattatt aaatactata tagaaacaac     3660

atcatttttc aaaattaggt caaagccttg tgtatcaagg gtttgatggt tctttgacag     3720

gtaaaaactc cttctgctat tattaaggtg tcgaatcaaa ataatagaat gctagagaac     3780

tagctcagaa ggagtttttt tgttgattta ttcatctgaa aatgattata gcatcctcga     3840

agataaaacc gcaacaggta aaaagcggga ttggaagggg aaaaagagac ggacgaacct     3900

catggcggag cattacgaag cgttagagag taagattggg gcaccttact atggcaaaaa     3960

ggctgaaaaa ctaattagtt gtgcagagta tctttcgttt aagagagacc cggagacggg     4020

caagttaaaa ctgtatcaag cccatttttg taaagtgagg ttatgtccga tgtgtgcgtg     4080

gcgcaggtcg ttaaaaattg cttatcacaa taagttgatc gtagaggaag ccaatagaca     4140

gtacggctgc ggatggattt ttctcacgct gacgattcga aatgtaaagg gagaacggct     4200

gaagccacaa atttctgcga tgatggaagg ctttaggaaa ctgttccagt acaaaaaagt     4260

aaaaacttcg gttcttggat ttttcagagc tttagagatt accaaaaatc atgaagaaga     4320

tacatatcat cctcattttc atgtgttgat accagtaagg aaaaattatt ttgggaaaaa     4380

ctatattaag caggcggagt ggacgagcct ttggaaaaag gcgatgaaat tggattacac     4440

tccaattgtc gatattcgtc gagtgaaagg taaagctaag attgacgctg aacagattga     4500

aaacgatgtg cggaacgcaa tgatggagca aaaagctgtt ctcgaaatct ctaaatatcc     4560

ggttaaggat acggatgttg tgcgcggtaa taaggtgact gaagacaatc tgaacacggt     4620

gctttacttg gatgatgcgt tggcagctcg aaggttaatt ggatacggtg gcattttgaa     4680

ggagatacat aaagagctga atcttggtga tgcggaggac ggcgatctgg tcaagattga     4740

ggaagaagat gacgaggttg caaatggtgc atttgaggtt atggcttatt ggcatcctgg     4800

cattaaaaat tacataatca aataaaaaaa gcagaccttt agaaggcctg cttttttaac     4860

taacccattt gtattgtgtt gaaatatgtt ttgtatggtg cactctcagt acaatctgct     4920

ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac     4980

gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca     5040

tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac     5100

gcctattttt ataggttaat gtcatgataa taatggtttc ttagcgattc acaaaaaata     5160

ggcacacgaa aaacaagtta agggatgcag tttatgcatc ccttaactta aaatactaaa     5220

aatgcccata ttttttcctc cttataaaat tagtataatt atagcacgag atctaaaagg     5280

atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg     5340

ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt     5400

ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg     5460

ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata     5520

ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca     5580

ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag     5640

tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc     5700

tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga     5760

tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg     5820

tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac     5880

gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg     5940

tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg     6000

ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct     6060

gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc     6120

gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc     6180

cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg     6240

ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta     6300

cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca     6360

ggaaacagct atgaccatga ttacgccgga tcc                                  6393


<210>  51
<211>  1069
<212>  DNA
<213>  Bacillus licheniformis

<400>  51
cggcatcaag tggatattcc cggaagcctc gctattttaa gctgtgacaa ccagccgatc       60

agcgaagtgc tcggcatttc aacaattgaa atcaatataa aggaaatggg gaagcgcgcg      120

tttcgccttc tgcaaaaaag aatcggcggc gccgggccgg aaaaactgac tgttccttac      180

aagctgatca aaagggcgac tgtgtaatca aaacatcaat ctttttccag acagtgatca      240

aaattcgaca tttttcatca aaaacgtcaa aataaattga cgcgctttca tgagttttgt      300

acgatatgag agattgatga caaaaaggag ctgaggatcg tgaacagatc ctttaccgtt      360

gaaaaggtac tcaacaacaa cgttttaatc gctctccatg atgattacag agaagttgtc      420

ttgattggca aaggaatcgg ttttggtaaa aagcgcggag atcttatcga acatgagaac      480

tacgaaaaaa tgtttatcct ggaaaatgat aaggaacaat cgcagtataa gaagctcctc      540

acttatgtcg atgaaaaaat ggttgatatc gccaatgatg tcatctacca tatcgcgcaa      600

aaaatcggcc agccgctgaa cgaacacatt catgtcgccc tgacggacca tatcgcattt      660

gcagttaagc gtctagaaaa gggatttgat atgaaaaatc cgtttttgct tgagacggaa      720

tcgctttatc cgaaggaata cgaagtcgcc aaggaagccg tcgatatgat taatgaaaaa      780

tccgacattc agctgcctga aggtgaaatc gggttcatcg cgcttcatat ccacagtgcg      840

atgacaaacc gcccgctttc tgaagtcaat cagcattcac aactgatctc caggcttgtc      900

caggtcatcg aagattcatt ccagatgcaa gtcaacaggg aaagcgtgaa ctatttgcgg      960

ctgatcaggc acttgcgctt tacgattgac aggataaaac gggacgagcc gattcaggaa     1020

ccggaaaaat taatgttgtt gttgaaaacg gaatatccgc tgtgttaca                 1069


<210>  52
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT locus forward primer

<400>  52
cggcatcaag tggatattcc                                                   20


<210>  53
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT locus reverse primer

<400>  53
tgtaacacag cggatattcc                                                   20


<210>  54
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT locus forward sequencing primer

<400>  54
tgaggatcgt gaacagatcc                                                   20


<210>  55
<211>  281
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glcT L67F

<400>  55

Val Asn Arg Ser Phe Thr Val Glu Lys Val Leu Asn Asn Asn Val Leu 
1               5                   10                  15      


Ile Ala Leu His Asp Asp Tyr Arg Glu Val Val Leu Ile Gly Lys Gly 
            20                  25                  30          


Ile Gly Phe Gly Lys Lys Arg Gly Asp Leu Ile Glu His Glu Asn Tyr 
        35                  40                  45              


Glu Lys Met Phe Ile Leu Glu Asn Asp Lys Glu Gln Ser Gln Tyr Lys 
    50                  55                  60                  


Lys Leu Phe Thr Tyr Val Asp Glu Lys Met Val Asp Ile Ala Asn Asp 
65                  70                  75                  80  


Val Ile Tyr His Ile Ala Gln Lys Ile Gly Gln Pro Leu Asn Glu His 
                85                  90                  95      


Ile His Val Ala Leu Thr Asp His Ile Ala Phe Ala Val Lys Arg Leu 
            100                 105                 110         


Glu Lys Gly Phe Asp Met Lys Asn Pro Phe Leu Leu Glu Thr Glu Ser 
        115                 120                 125             


Leu Tyr Pro Lys Glu Tyr Glu Val Ala Lys Glu Ala Val Asp Met Ile 
    130                 135                 140                 


Asn Glu Lys Ser Asp Ile Gln Leu Pro Glu Gly Glu Ile Gly Phe Ile 
145                 150                 155                 160 


Ala Leu His Ile His Ser Ala Met Thr Asn Arg Pro Leu Ser Glu Val 
                165                 170                 175     


Asn Gln His Ser Gln Leu Ile Ser Arg Leu Val Gln Val Ile Glu Asp 
            180                 185                 190         


Ser Phe Gln Met Gln Val Asn Arg Glu Ser Val Asn Tyr Leu Arg Leu 
        195                 200                 205             


Ile Arg His Leu Arg Phe Thr Ile Asp Arg Ile Lys Arg Asp Glu Pro 
    210                 215                 220                 


Ile Gln Glu Pro Glu Lys Leu Met Leu Leu Leu Lys Thr Glu Tyr Pro 
225                 230                 235                 240 


Leu Cys Tyr Asn Thr Ala Trp Lys Met Ile Lys Ile Leu Gln Gln Ala 
                245                 250                 255     


Leu Lys Lys Pro Val His Glu Ala Glu Ala Val Tyr Leu Thr Leu His 
            260                 265                 270         


Leu Tyr Arg Leu Thr Asn Lys Ile Ser 
        275                 280     


<210>  56
<211>  846
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  glcT ORF encoding L67F

<400>  56
gtgaacagat cctttaccgt tgaaaaggta ctcaacaaca acgttttaat cgctctccat       60

gatgattaca gagaagttgt cttgattggc aaaggaatcg gttttggtaa aaagcgcgga      120

gatcttatcg aacatgagaa ctacgaaaaa atgtttatcc tggaaaatga taaggaacaa      180

tcgcagtata agaagctctt cacttatgtc gatgaaaaaa tggttgatat cgccaatgat      240

gtcatctacc atatcgcgca aaaaatcggc cagccgctga acgaacacat tcatgtcgcc      300

ctgacggacc atatcgcatt tgcagttaag cgtctagaaa agggatttga tatgaaaaat      360

ccgtttttgc ttgagacgga atcgctttat ccgaaggaat acgaagtcgc caaggaagcc      420

gtcgatatga ttaatgaaaa atccgacatt cagctgcctg aaggtgaaat cgggttcatc      480

gcgcttcata tccacagtgc gatgacaaac cgcccgcttt ctgaagtcaa tcagcattca      540

caactgatct ccaggcttgt ccaggtcatc gaagattcat tccagatgca agtcaacagg      600

gaaagcgtga actatttgcg gctgatcagg cacttgcgct ttacgattga caggataaaa      660

cgggacgagc cgattcagga accggaaaaa ttaatgttgt tgttgaaaac ggaatatccg      720

ctgtgttaca atactgcttg gaagatgatc aagatcttgc agcaagcgct caagaaaccg      780

gttcatgagg cagaagccgt ttatttgaca ttgcatttgt accgtttgac taataaaatt      840

tcataa                                                                 846


<210>  57
<211>  1164
<212>  DNA
<213>  Bacillus licheniformis

<400>  57
gcgaatcgaa aacggaaagc gcggcgtgcc gaagccggcg acgatcagaa aactggcgga       60

cgctttgaaa gtcccgtatg aggaactgat ggcatctgca ggctatatca gcgcgtctac      120

agtccaggaa gcaagaagca gctatgattc catttacgac atcgtgtcac agtacgattt      180

agaggacctt tctctgtttg acagcgaaaa gtggaaggtg ctttcaaaaa aagacatcga      240

aaacctggac aaatatttcg actttctcgt gcaggaagca agcagccgaa acaaaaactg      300

aatacttctc cgcggcacac tctcctctct atcattttcg tctgtttacg atcctgctgt      360

tattttatcc cttatgttaa cttttgtcaa tatttttcct gtctaagtat ttcctatagt      420

caacatttgt attaaaatgt tcatatcatg aatttgcggg ggggatggcg atgacaaggt      480

tcggcgagcg gctcaaagag ctgagggaac aaagaagcct gtcggttaat cagcttgcca      540

tgtatgccgg tgtgagcgcc gcagccattt ccagagccgc agccatttcc agaatcgaaa      600

acggccaccg cggcgttccc aagcccgcga cgatcagaaa attggccgag gctctgaaaa      660

tgccgtacga gcagctcatg gatattgccg gttatatgag agctgacgag attcgcgaac      720

agccgcgcgg ctatgtcacg atgcaggaga tcgcggccaa gcacggcgtc gaagacctgt      780

ggctgtttaa acccgagaaa tgggactgtt tgtcccgcga agacctgctc aacctcgaac      840

agtattttca ttttttggtt aatgaagcga agaagcgcca atcataaaaa gccgaatttc      900

ccttttagga gaagttcggc ttttttcggc tgccttaagc ggcatccgga ttcggcgtct      960

tgcctttatg atgcttaacg gggctcagcg cacgctcgag ccatcccatg aacagatcgg     1020

cgatgatcgc catcagcgcc gtcgggatcg cgcctgctag aatgatcgct gttccgttgg     1080

tcgcgtttga tcccctgaca atgatatccc cgaggccgcc tgcgccgaca aacgtgccga     1140

tggccgtaat gccgatcgcg atga                                            1164


<210>  58
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 locus forward primer

<400>  58
gcgaatcgaa aacggaaagc                                                   20


<210>  59
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 locus reverse primer

<400>  59
tcatcgcgat cggcattacg                                                   20


<210>  60
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  rghR2 locus sequencing primer

<400>  60
tttcgacttt ctcgtgcagg                                                   20


<210>  61
<211>  1146
<212>  DNA
<213>  Bacillus licheniformis

<400>  61
gcgaatcgaa aacggaaagc gcggcgtgcc gaagccggcg acgatcagaa aactggcgga       60

cgctttgaaa gtcccgtatg aggaactgat ggcatctgca ggctatatca gcgcgtctac      120

agtccaggaa gcaagaagca gctatgattc catttacgac atcgtgtcac agtacgattt      180

agaggacctt tctctgtttg acagcgaaaa gtggaaggtg ctttcaaaaa aagacatcga      240

aaacctggac aaatatttcg actttctcgt gcaggaagca agcagccgaa acaaaaactg      300

aatacttctc cgcggcacac tctcctctct atcattttcg tctgtttacg atcctgctgt      360

tattttatcc cttatgttaa cttttgtcaa tatttttcct gtctaagtat ttcctatagt      420

caacatttgt attaaaatgt tcatatcatg aatttgcggg ggggatggcg atgacaaggt      480

tcggcgagcg gctcaaagag ctgagggaac aaagaagcct gtcggttaat cagcttgcca      540

tgtatgccgg tgtgagcgcc gcagccattt ccagaatcga aaacggccac cgcggcgttc      600

ccaagcccgc gacgatcaga aaattggccg aggctctgaa aatgccgtac gagcagctca      660

tggatattgc cggttatatg agagctgacg agattcgcga acagccgcgc ggctatgtca      720

cgatgcagga gatcgcggcc aagcacggcg tcgaagacct gtggctgttt aaacccgaga      780

aatgggactg tttgtcccgc gaagacctgc tcaacctcga acagtatttt cattttttgg      840

ttaatgaagc gaagaagcgc caatcataaa aagccgaatt tcccttttag gagaagttcg      900

gcttttttcg gctgccttaa gcggcatccg gattcggcgt cttgccttta tgatgcttaa      960

cggggctcag cgcacgctcg agccatccca tgaacagatc ggcgatgatc gccatcagcg     1020

ccgtcgggat cgcgcctgct agaatgatcg ctgttccgtt ggtcgcgttt gatcccctga     1080

caatgatatc cccgaggccg cctgcgccga caaacgtgcc gatggccgta atgccgatcg     1140

cgatga                                                                1146


<210>  62
<211>  58
<212>  DNA
<213>  Bacillus subtilis

<400>  62
acagaatagt cttttaagta agtctactct gaattttttt aaaaggagag ggtaaaga         58


<210>  63
<211>  57
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  aprE mod-5' UTR

<400>  63
acagaatagt cttttaagta agtctactct gaattttttt aaaaggagag ggtaaag          57


<210>  64
<211>  6208
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  WT 5' UTR expression construct

<400>  64
ggctatgacg aaacgattgc agcctatgag gcgagtctcg aaaagctcgg acttgactac       60

cttgatttat acctgatcca ctggcctgtt gaaggacgct acaaagcggc gtggaaagcg      120

cttgaaacac tttatgaaca aggacgcgta aaagcaatcg gagtgagcaa ttttcagatt      180

caccatctgg aagacttgct gaaagatgcc gccgtcaaac cggcgatcaa ccaggttgag      240

tatcatccgc ggctgacgca gaaagagctg caagcgtttt gccgtgcgca cggcatccag      300

ctgcaagcat ggtcgccgct gatgcaaggc caattgctca gccatccact gctgaaagat      360

atcgcggaca agtacggcaa gacaccggcc caagtcattt tgcgctggga tttgcaaaac      420

ggggtcgtta cgattccgaa gtcgactaaa gcggagcgga ttgcccaaaa cgcggacata      480

tttgattttg aactgaccac cgaggaaatg aagcaaattg acgcgctgaa tgaaaacacc      540

cgtgtcggcc ctgatcccga taactttgac ttttaacaaa acggccccgt tcgacattcg      600

aacggggctt taattgaatt gtgcggttac accgccggac tccatcatca tcagttcttt      660

tttcatatcc aatccgcccc ggtatcccgt gagctgcccg cttttaccga taacccgatg      720

gcaaggcacc accattaaca gcggatttgc gccgatcgcc gcgcctactg cccgcacagc      780

ggcctgcttt tcaatatgct cggcgatatc ggaataggag caagtgctgc cgtaagggat      840

ttcggagagc gccttccaca ctgccagctg aaaaggcgtg ccggcaaggt cgacaggaaa      900

gctgaaatga gttcgcttgc cgttcaaata cgcctgcagc tgctcggcgt attctgccaa      960

tcctttgtca tcccgaatga aaactggctg tgtaaatctt ttttcagccc aagcggccaa     1020

atcctcgaag ccttgattcc atccccctgt aaaacagagc ccgcgggcag tcgccccaat     1080

gtgaatctgc caacctcggc aaataagcgt acgccagtat acgatttgat cgtccatatg     1140

tttacctccg tttcatttgc cggtacgacg tcggcgattg cccagtcttc tttttaaaca     1200

aagaggcaaa atattccgca ttcgcaatgc ctaccattga agcgatttct gcgatcgatc     1260

gttctgaatg agcaagcaaa tcgaccgctt tctcaatcct tttctgcagg atgtattctg     1320

ccggcgagac gcctttgatt cgtttaaatg tccgctgcag gtgaaaaggg ctgatatggc     1380

acctgtcagc caaagcttgc agagacagcg gatcgcgata agattcctcg atgatttcca     1440

ccacacgctg tgccagctct tcatccggca gcagcgcccc ggccggattg cagcgtttgc     1500

aggggcggta cccttctgat aaagcatctt ttgcattgaa aaagatctgc acattgtcga     1560

tttgcggaac tctcgatttg caggaagggc ggcaaaatat gccggtcgtt ttgaccgcgt     1620

aataaaaaac tccgtcatag gcggaatcgt tttccgtaat cgcccgccac atttcaggcg     1680

tcaatcgtga tttgctgttc atatcttcac cccgatctat gtcagtataa cctatatgac     1740

agccggaggt ggagaggcgg agaacggcac agcaagaaga caaagaagaa gagagactgt     1800

tgcctggacc tccgaaacgc gctacaattc atttacaaca caggatgggg tgagaatatt     1860

gccggaatca gtgaagcagg cctcctaaaa taaaaatcta tattttagga ggtaaaacat     1920

gaattttcaa acaatcgagc ttgacacatg gtatagaaaa tcttattttg accattacat     1980

gaaggaagcg aaatgttctt tcagcatcac ggcaaacgtc aatgtgacaa atttgctcgc     2040

cgtgctcaag aaaaagaagc tcaagctgta tccggctttt atttatatcg tatcaagggt     2100

cattcattcg cgccctgagt ttagaacaac gtttgatgac aaaggacagc tgggttattg     2160

ggaacaaatg catccgtgct atgcgatttt tcatcaggac gaccaaacgt tttccgccct     2220

ctggacggaa tactcagacg atttttcgca gttttatcat caatatcttc tggacgccga     2280

gcgctttgga gacaaaaggg gcctttgggc taagccggac atcccgccca atacgttttc     2340

agtttcttct attccatggg tgcgcttttc aaacttcaat ttaaaccttg ataacagcga     2400

acacttgctg ccgattatta caaacgggaa atacttttca gaaggcaggg aaacattttt     2460

gcccgtttcc ttgcaagttc accatgcagt gtgtgacggc tatcatgccg gcgcttttat     2520

aaacgagttg gaacggcttg ccgccgattg tgaggagtgg cttgtgtgac agaggaaagg     2580

ccgatatgat tcggcctttt ttatatgtac ttcttagcgg gtctcttaac ccccctcgag     2640

gtcgctgata aacagctgac atcaatatcc tattttttca aaaaatattt taaaaagttg     2700

ttgacttaaa agaagctaaa tgttatagta ataaaacaga atagtctttt aagtaagtct     2760

actctgaatt tttttaaaag gagagggtaa agaatgaaac aacaaaaacg gctttacgcc     2820

cgattgctga cgctgttatt tgcgctcatc ttcttgctgc ctcattctgc agctagcgca     2880

gccgcaccgt ttaacggtac catgatgcag tattttgaat ggtacttgcc ggatgatggc     2940

acgttatgga ccaaagtggc caatgaagcc aacaacttat ccagccttgg catcaccgct     3000

ctttggctgc cgcccgctta caaaggaaca agccgcagcg acgtagggta cggagtatac     3060

gacttgtatg acctcggcga attcaatcaa aaagggaccg tccgcacaaa atatggaaca     3120

aaagctcaat atcttcaagc cattcaagcc gcccacgccg ctggaatgca agtgtacgcc     3180

gatgtcgtgt tcgaccataa aggcggcgct gacggcacgg aatgggtgga cgccgtcgaa     3240

gtcaatccgt ccgaccgcaa ccaagaaatc tcgggcacct atcaaatcca agcatggacg     3300

aaatttgatt ttcccgggcg gggcaacacc tactccagct ttaagtggcg ctggtaccat     3360

tttgacggcg ttgattggga cgaaagccga aaattaagcc gcatttacaa attcaggggc     3420

atcggcaaag cgtgggattg gccggtagac acagaaaacg gaaactatga ctacttaatg     3480

tatgccgacc ttgatatgga tcatcccgaa gtcgtgaccg agctgaaaaa ctgggggaaa     3540

tggtatgtca acacaacgaa cattgatggg ttccggcttg atgccgtcaa gcatattaag     3600

ttcagttttt ttcctgattg gttgtcgtat gtgcgttctc agactggcaa gccgctattt     3660

accgtcgggg aatattggag ctatgacatc aacaagttgc acaattacat tacgaaaaca     3720

aacggaacga tgtctttgtt tgatgccccg ttacacaaca aattttatac cgcttccaaa     3780

tcagggggcg catttgatat gcgcacgtta atgaccaata ctctcatgaa agatcaaccg     3840

acattggccg tcaccttcgt tgataatcat gacaccgaac ccggccaagc gcttcagtca     3900

tgggtcgacc catggttcaa accgttggct tacgccttta ttctaactcg gcaggaagga     3960

tacccgtgcg tcttttatgg tgactattat ggcattccac aatataacat tccttcgctg     4020

aaaagcaaaa tcgatccgct cctcatcgcg cgcagggatt atgcttacgg aacgcaacat     4080

gattatcttg atcactccga catcatcggg tggacaaggg aaggggtcac tgaaaaacca     4140

ggatccgggc tggccgcact gatcaccgat gggccgggag gaagcaaatg gatgtacgtt     4200

ggcaaacaac acgctggaaa agtgttctat gaccttaccg gcaaccggag tgacaccgtc     4260

accatcaaca gtgatggatg gggggaattc aaagtcaatg gcggttcggt ttcggtttgg     4320

gttcctagaa aaacgaccta aaagcttctc gaggttaaca gaggacggat ttcctgaagg     4380

aaatccgttt ttttattttt aacatctctc actgctgtgt gattttactc acggcatttg     4440

gaacgccggc tctcaacaaa ctttctgtag tgaaaatcat gaaccaaacg gatcgtcggc     4500

ctgattaaca gctgaaagct gccgatcaca aacatccata gtcccgccgg cttcagttcc     4560

tcggagaaaa agcagaagct cccgacaagg aataaaaggc cgatgagaaa atcgtttaat     4620

gtatgtagaa ctttgtatct ttttttgaaa aagagttcat atcgattgtt attgttttgc     4680

ggcattgctt gatcactcca atccttttat ttaccctgcc ggaagccgga gtgaaacgcc     4740

ggtatacata ggatttatga attaggaaaa catatgggga aataaaccat ccaggagtga     4800

aaaatatgcg gttattcata tgtgcatcgt gcctgttcgg cttgattgtt ccgtcatttg     4860

aaacgaaagc gctgacgttt gaagaattgc cggttaaaca agcttcaaaa caatgggaag     4920

ttcaaatcgg taaagccgaa gccggaaacg gaatggcgaa accggaaaaa ggagcgtttc     4980

atacttatgc tgtcgaaatc aaaaacattg gacacgatgt ggcttcggcg gaaatttttg     5040

tctatcggaa cgagcctaat tcttcaacga aattttcgct ttggaacatt cctcacgaaa     5100

atccggtttc tttagccaaa agcttaaatc acggaagctc tgtcaagcac cgcaatctgc     5160

ttatggcaga gaatgcgacc gaattggaag tggacatgat ttggacggaa aaaggaagcg     5220

aaggcagact tttaaaggaa acgttcattt tcaagggaga tgaatcatga agaaaaaatg     5280

gccgttcatc gtcaacggtc tttttttaat gacttaggca gccgatcgtt cggccatacg     5340

atatcgaagc gacctcgaac cagcagagct cgtcacaaaa catttgcatt taaagaaaaa     5400

tacaggatgt tttcaccaat atttttctca atgatgatac actattgaca agctgctact     5460

ttgggagggt gtttccatag atgccgatga agcaaaaaca ccaaatgtgt catgagagct     5520

ctctctaatc gatataaaag tagggtgaac cggggttgtc aatctgtaaa agatcttttt     5580

ttatcccgtg atacgctttt ggaattctga atcttcaaga aagtccccag ccttttgctg     5640

atcaatcgag aacaaaggat gatacatatg aaaagaatag ataaaatcta ccatcagctg     5700

ctggataatt ttcgcgaaaa gaatatcaat cagcttttaa agatacaagg gaattcggct     5760

aaagaaatcg ccgggcagct gcaaatggag cgttccaatg tcagctttga attaaacaat     5820

ctggttcggg ccaaaaaggt gatcaagatt aaaacgttcc ccgtccgcta catcccggtg     5880

gaaattgttg aaaacgtctt gaacatcaaa tggaattcag agttgatgga ggttgaagaa     5940

ctgaggcggc tggctgacgg ccaaaaaaag ccggcgcgca atatatccgc cgatcccctc     6000

gagctcatga tcggggctaa agggagcttg aaaaaggcaa tttctcaggc gaaagcggca     6060

gtcttttatc ctccgcacgg cttgcatatg ctgctgctcg ggccgacggg ttcggggaaa     6120

tcgctgtttg cgaatcggat ctaccagttc gccgtttatt ctgacatatt gaagcccgat     6180

tccccgttca tcacattcaa ctgtgcag                                        6208


<210>  65
<211>  6207
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Mod 5' UTR expression construct

<400>  65
ggctatgacg aaacgattgc agcctatgag gcgagtctcg aaaagctcgg acttgactac       60

cttgatttat acctgatcca ctggcctgtt gaaggacgct acaaagcggc gtggaaagcg      120

cttgaaacac tttatgaaca aggacgcgta aaagcaatcg gagtgagcaa ttttcagatt      180

caccatctgg aagacttgct gaaagatgcc gccgtcaaac cggcgatcaa ccaggttgag      240

tatcatccgc ggctgacgca gaaagagctg caagcgtttt gccgtgcgca cggcatccag      300

ctgcaagcat ggtcgccgct gatgcaaggc caattgctca gccatccact gctgaaagat      360

atcgcggaca agtacggcaa gacaccggcc caagtcattt tgcgctggga tttgcaaaac      420

ggggtcgtta cgattccgaa gtcgactaaa gcggagcgga ttgcccaaaa cgcggacata      480

tttgattttg aactgaccac cgaggaaatg aagcaaattg acgcgctgaa tgaaaacacc      540

cgtgtcggcc ctgatcccga taactttgac ttttaacaaa acggccccgt tcgacattcg      600

aacggggctt taattgaatt gtgcggttac accgccggac tccatcatca tcagttcttt      660

tttcatatcc aatccgcccc ggtatcccgt gagctgcccg cttttaccga taacccgatg      720

gcaaggcacc accattaaca gcggatttgc gccgatcgcc gcgcctactg cccgcacagc      780

ggcctgcttt tcaatatgct cggcgatatc ggaataggag caagtgctgc cgtaagggat      840

ttcggagagc gccttccaca ctgccagctg aaaaggcgtg ccggcaaggt cgacaggaaa      900

gctgaaatga gttcgcttgc cgttcaaata cgcctgcagc tgctcggcgt attctgccaa      960

tcctttgtca tcccgaatga aaactggctg tgtaaatctt ttttcagccc aagcggccaa     1020

atcctcgaag ccttgattcc atccccctgt aaaacagagc ccgcgggcag tcgccccaat     1080

gtgaatctgc caacctcggc aaataagcgt acgccagtat acgatttgat cgtccatatg     1140

tttacctccg tttcatttgc cggtacgacg tcggcgattg cccagtcttc tttttaaaca     1200

aagaggcaaa atattccgca ttcgcaatgc ctaccattga agcgatttct gcgatcgatc     1260

gttctgaatg agcaagcaaa tcgaccgctt tctcaatcct tttctgcagg atgtattctg     1320

ccggcgagac gcctttgatt cgtttaaatg tccgctgcag gtgaaaaggg ctgatatggc     1380

acctgtcagc caaagcttgc agagacagcg gatcgcgata agattcctcg atgatttcca     1440

ccacacgctg tgccagctct tcatccggca gcagcgcccc ggccggattg cagcgtttgc     1500

aggggcggta cccttctgat aaagcatctt ttgcattgaa aaagatctgc acattgtcga     1560

tttgcggaac tctcgatttg caggaagggc ggcaaaatat gccggtcgtt ttgaccgcgt     1620

aataaaaaac tccgtcatag gcggaatcgt tttccgtaat cgcccgccac atttcaggcg     1680

tcaatcgtga tttgctgttc atatcttcac cccgatctat gtcagtataa cctatatgac     1740

agccggaggt ggagaggcgg agaacggcac agcaagaaga caaagaagaa gagagactgt     1800

tgcctggacc tccgaaacgc gctacaattc atttacaaca caggatgggg tgagaatatt     1860

gccggaatca gtgaagcagg cctcctaaaa taaaaatcta tattttagga ggtaaaacat     1920

gaattttcaa acaatcgagc ttgacacatg gtatagaaaa tcttattttg accattacat     1980

gaaggaagcg aaatgttctt tcagcatcac ggcaaacgtc aatgtgacaa atttgctcgc     2040

cgtgctcaag aaaaagaagc tcaagctgta tccggctttt atttatatcg tatcaagggt     2100

cattcattcg cgccctgagt ttagaacaac gtttgatgac aaaggacagc tgggttattg     2160

ggaacaaatg catccgtgct atgcgatttt tcatcaggac gaccaaacgt tttccgccct     2220

ctggacggaa tactcagacg atttttcgca gttttatcat caatatcttc tggacgccga     2280

gcgctttgga gacaaaaggg gcctttgggc taagccggac atcccgccca atacgttttc     2340

agtttcttct attccatggg tgcgcttttc aaacttcaat ttaaaccttg ataacagcga     2400

acacttgctg ccgattatta caaacgggaa atacttttca gaaggcaggg aaacattttt     2460

gcccgtttcc ttgcaagttc accatgcagt gtgtgacggc tatcatgccg gcgcttttat     2520

aaacgagttg gaacggcttg ccgccgattg tgaggagtgg cttgtgtgac agaggaaagg     2580

ccgatatgat tcggcctttt ttatatgtac ttcttagcgg gtctcttaac ccccctcgag     2640

gtcgctgata aacagctgac atcaatatcc tattttttca aaaaatattt taaaaagttg     2700

ttgacttaaa agaagctaaa tgttatagta ataaaacaga atagtctttt aagtaagtct     2760

actctgaatt tttttaaaag gagagggtaa agatgaaaca acaaaaacgg ctttacgccc     2820

gattgctgac gctgttattt gcgctcatct tcttgctgcc tcattctgca gctagcgcag     2880

ccgcaccgtt taacggtacc atgatgcagt attttgaatg gtacttgccg gatgatggca     2940

cgttatggac caaagtggcc aatgaagcca acaacttatc cagccttggc atcaccgctc     3000

tttggctgcc gcccgcttac aaaggaacaa gccgcagcga cgtagggtac ggagtatacg     3060

acttgtatga cctcggcgaa ttcaatcaaa aagggaccgt ccgcacaaaa tatggaacaa     3120

aagctcaata tcttcaagcc attcaagccg cccacgccgc tggaatgcaa gtgtacgccg     3180

atgtcgtgtt cgaccataaa ggcggcgctg acggcacgga atgggtggac gccgtcgaag     3240

tcaatccgtc cgaccgcaac caagaaatct cgggcaccta tcaaatccaa gcatggacga     3300

aatttgattt tcccgggcgg ggcaacacct actccagctt taagtggcgc tggtaccatt     3360

ttgacggcgt tgattgggac gaaagccgaa aattaagccg catttacaaa ttcaggggca     3420

tcggcaaagc gtgggattgg ccggtagaca cagaaaacgg aaactatgac tacttaatgt     3480

atgccgacct tgatatggat catcccgaag tcgtgaccga gctgaaaaac tgggggaaat     3540

ggtatgtcaa cacaacgaac attgatgggt tccggcttga tgccgtcaag catattaagt     3600

tcagtttttt tcctgattgg ttgtcgtatg tgcgttctca gactggcaag ccgctattta     3660

ccgtcgggga atattggagc tatgacatca acaagttgca caattacatt acgaaaacaa     3720

acggaacgat gtctttgttt gatgccccgt tacacaacaa attttatacc gcttccaaat     3780

cagggggcgc atttgatatg cgcacgttaa tgaccaatac tctcatgaaa gatcaaccga     3840

cattggccgt caccttcgtt gataatcatg acaccgaacc cggccaagcg cttcagtcat     3900

gggtcgaccc atggttcaaa ccgttggctt acgcctttat tctaactcgg caggaaggat     3960

acccgtgcgt cttttatggt gactattatg gcattccaca atataacatt ccttcgctga     4020

aaagcaaaat cgatccgctc ctcatcgcgc gcagggatta tgcttacgga acgcaacatg     4080

attatcttga tcactccgac atcatcgggt ggacaaggga aggggtcact gaaaaaccag     4140

gatccgggct ggccgcactg atcaccgatg ggccgggagg aagcaaatgg atgtacgttg     4200

gcaaacaaca cgctggaaaa gtgttctatg accttaccgg caaccggagt gacaccgtca     4260

ccatcaacag tgatggatgg ggggaattca aagtcaatgg cggttcggtt tcggtttggg     4320

ttcctagaaa aacgacctaa aagcttctcg aggttaacag aggacggatt tcctgaagga     4380

aatccgtttt tttattttta acatctctca ctgctgtgtg attttactca cggcatttgg     4440

aacgccggct ctcaacaaac tttctgtagt gaaaatcatg aaccaaacgg atcgtcggcc     4500

tgattaacag ctgaaagctg ccgatcacaa acatccatag tcccgccggc ttcagttcct     4560

cggagaaaaa gcagaagctc ccgacaagga ataaaaggcc gatgagaaaa tcgtttaatg     4620

tatgtagaac tttgtatctt tttttgaaaa agagttcata tcgattgtta ttgttttgcg     4680

gcattgcttg atcactccaa tccttttatt taccctgccg gaagccggag tgaaacgccg     4740

gtatacatag gatttatgaa ttaggaaaac atatggggaa ataaaccatc caggagtgaa     4800

aaatatgcgg ttattcatat gtgcatcgtg cctgttcggc ttgattgttc cgtcatttga     4860

aacgaaagcg ctgacgtttg aagaattgcc ggttaaacaa gcttcaaaac aatgggaagt     4920

tcaaatcggt aaagccgaag ccggaaacgg aatggcgaaa ccggaaaaag gagcgtttca     4980

tacttatgct gtcgaaatca aaaacattgg acacgatgtg gcttcggcgg aaatttttgt     5040

ctatcggaac gagcctaatt cttcaacgaa attttcgctt tggaacattc ctcacgaaaa     5100

tccggtttct ttagccaaaa gcttaaatca cggaagctct gtcaagcacc gcaatctgct     5160

tatggcagag aatgcgaccg aattggaagt ggacatgatt tggacggaaa aaggaagcga     5220

aggcagactt ttaaaggaaa cgttcatttt caagggagat gaatcatgaa gaaaaaatgg     5280

ccgttcatcg tcaacggtct ttttttaatg acttaggcag ccgatcgttc ggccatacga     5340

tatcgaagcg acctcgaacc agcagagctc gtcacaaaac atttgcattt aaagaaaaat     5400

acaggatgtt ttcaccaata tttttctcaa tgatgataca ctattgacaa gctgctactt     5460

tgggagggtg tttccataga tgccgatgaa gcaaaaacac caaatgtgtc atgagagctc     5520

tctctaatcg atataaaagt agggtgaacc ggggttgtca atctgtaaaa gatctttttt     5580

tatcccgtga tacgcttttg gaattctgaa tcttcaagaa agtccccagc cttttgctga     5640

tcaatcgaga acaaaggatg atacatatga aaagaataga taaaatctac catcagctgc     5700

tggataattt tcgcgaaaag aatatcaatc agcttttaaa gatacaaggg aattcggcta     5760

aagaaatcgc cgggcagctg caaatggagc gttccaatgt cagctttgaa ttaaacaatc     5820

tggttcgggc caaaaaggtg atcaagatta aaacgttccc cgtccgctac atcccggtgg     5880

aaattgttga aaacgtcttg aacatcaaat ggaattcaga gttgatggag gttgaagaac     5940

tgaggcggct ggctgacggc caaaaaaagc cggcgcgcaa tatatccgcc gatcccctcg     6000

agctcatgat cggggctaaa gggagcttga aaaaggcaat ttctcaggcg aaagcggcag     6060

tcttttatcc tccgcacggc ttgcatatgc tgctgctcgg gccgacgggt tcggggaaat     6120

cgctgtttgc gaatcggatc taccagttcg ccgtttattc tgacatattg aagcccgatt     6180

ccccgttcat cacattcaac tgtgcag                                         6207


<210>  66
<211>  1703
<212>  DNA
<213>  Bacillus licheniformis

<400>  66
ggctatgacg aaacgattgc agcctatgag gcgagtctcg aaaagctcgg acttgactac       60

cttgatttat acctgatcca ctggcctgtt gaaggacgct acaaagcggc gtggaaagcg      120

cttgaaacac tttatgaaca aggacgcgta aaagcaatcg gagtgagcaa ttttcagatt      180

caccatctgg aagacttgct gaaagatgcc gccgtcaaac cggcgatcaa ccaggttgag      240

tatcatccgc ggctgacgca gaaagagctg caagcgtttt gccgtgcgca cggcatccag      300

ctgcaagcat ggtcgccgct gatgcaaggc caattgctca gccatccact gctgaaagat      360

atcgcggaca agtacggcaa gacaccggcc caagtcattt tgcgctggga tttgcaaaac      420

ggggtcgtta cgattccgaa gtcgactaaa gcggagcgga ttgcccaaaa cgcggacata      480

tttgattttg aactgaccac cgaggaaatg aagcaaattg acgcgctgaa tgaaaacacc      540

cgtgtcggcc ctgatcccga taactttgac ttttaacaaa acggccccgt tcgacattcg      600

aacggggctt taattgaatt gtgcggttac accgccggac tccatcatca tcagttcttt      660

tttcatatcc aatccgcccc ggtatcccgt gagctgcccg cttttaccga taacccgatg      720

gcaaggcacc accattaaca gcggatttgc gccgatcgcc gcgcctactg cccgcacagc      780

ggcctgcttt tcaatatgct cggcgatatc ggaataggag caagtgctgc cgtaagggat      840

ttcggagagc gccttccaca ctgccagctg aaaaggcgtg ccggcaaggt cgacaggaaa      900

gctgaaatga gttcgcttgc cgttcaaata cgcctgcagc tgctcggcgt attctgccaa      960

tcctttgtca tcccgaatga aaactggctg tgtaaatctt ttttcagccc aagcggccaa     1020

atcctcgaag ccttgattcc atccccctgt aaaacagagc ccgcgggcag tcgccccaat     1080

gtgaatctgc caacctcggc aaataagcgt acgccagtat acgatttgat cgtccatatg     1140

tttacctccg tttcatttgc cggtacgacg tcggcgattg cccagtcttc tttttaaaca     1200

aagaggcaaa atattccgca ttcgcaatgc ctaccattga agcgatttct gcgatcgatc     1260

gttctgaatg agcaagcaaa tcgaccgctt tctcaatcct tttctgcagg atgtattctg     1320

ccggcgagac gcctttgatt cgtttaaatg tccgctgcag gtgaaaaggg ctgatatggc     1380

acctgtcagc caaagcttgc agagacagcg gatcgcgata agattcctcg atgatttcca     1440

ccacacgctg tgccagctct tcatccggca gcagcgcccc ggccggattg cagcgtttgc     1500

aggggcggta cccttctgat aaagcatctt ttgcattgaa aaagatctgc acattgtcga     1560

tttgcggaac tctcgatttg caggaagggc ggcaaaatat gccggtcgtt ttgaccgcgt     1620

aataaaaaac tccgtcatag gcggaatcgt tttccgtaat cgcccgccac atttcaggcg     1680

tcaatcgtga tttgctgttc ata                                             1703


<210>  67
<211>  937
<212>  DNA
<213>  Bacillus licheniformis

<400>  67
atcttcaccc cgatctatgt cagtataacc tatatgacag ccggaggtgg agaggcggag       60

aacggcacag caagaagaca aagaagaaga gagactgttg cctggacctc cgaaacgcgc      120

tacaattcat ttacaacaca ggatggggtg agaatattgc cggaatcagt gaagcaggcc      180

tcctaaaata aaaatctata ttttaggagg taaaacatga attttcaaac aatcgagctt      240

gacacatggt atagaaaatc ttattttgac cattacatga aggaagcgaa atgttctttc      300

agcatcacgg caaacgtcaa tgtgacaaat ttgctcgccg tgctcaagaa aaagaagctc      360

aagctgtatc cggcttttat ttatatcgta tcaagggtca ttcattcgcg ccctgagttt      420

agaacaacgt ttgatgacaa aggacagctg ggttattggg aacaaatgca tccgtgctat      480

gcgatttttc atcaggacga ccaaacgttt tccgccctct ggacggaata ctcagacgat      540

ttttcgcagt tttatcatca atatcttctg gacgccgagc gctttggaga caaaaggggc      600

ctttgggcta agccggacat cccgcccaat acgttttcag tttcttctat tccatgggtg      660

cgcttttcaa acttcaattt aaaccttgat aacagcgaac acttgctgcc gattattaca      720

aacgggaaat acttttcaga aggcagggaa acatttttgc ccgtttcctt gcaagttcac      780

catgcagtgt gtgacggcta tcatgccggc gcttttataa acgagttgga acggcttgcc      840

gccgattgtg aggagtggct tgtgtgacag aggaaaggcc gatatgattc ggcctttttt      900

atatgtactt cttagcgggt ctcttaaccc ccctcga                               937


<210>  68
<211>  95
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  spoVGrrnIp hybrid promoter

<400>  68
gtcgctgata aacagctgac atcaatatcc tattttttca aaaaatattt taaaaagttg       60

ttgacttaaa agaagctaaa tgttatagta ataaa                                  95


<210>  69
<211>  87
<212>  DNA
<213>  Bacillus licheniformis

<400>  69
atgaaacaac aaaaacggct ttacgcccga ttgctgacgc tgttatttgc gctcatcttc       60

ttgctgcctc attctgcagc tagcgca                                           87


<210>  70
<211>  1461
<212>  DNA
<213>  G. stearothermophilus

<400>  70
gccgcaccgt ttaacggtac catgatgcag tattttgaat ggtacttgcc ggatgatggc       60

acgttatgga ccaaagtggc caatgaagcc aacaacttat ccagccttgg catcaccgct      120

ctttggctgc cgcccgctta caaaggaaca agccgcagcg acgtagggta cggagtatac      180

gacttgtatg acctcggcga attcaatcaa aaagggaccg tccgcacaaa atatggaaca      240

aaagctcaat atcttcaagc cattcaagcc gcccacgccg ctggaatgca agtgtacgcc      300

gatgtcgtgt tcgaccataa aggcggcgct gacggcacgg aatgggtgga cgccgtcgaa      360

gtcaatccgt ccgaccgcaa ccaagaaatc tcgggcacct atcaaatcca agcatggacg      420

aaatttgatt ttcccgggcg gggcaacacc tactccagct ttaagtggcg ctggtaccat      480

tttgacggcg ttgattggga cgaaagccga aaattaagcc gcatttacaa attcaggggc      540

atcggcaaag cgtgggattg gccggtagac acagaaaacg gaaactatga ctacttaatg      600

tatgccgacc ttgatatgga tcatcccgaa gtcgtgaccg agctgaaaaa ctgggggaaa      660

tggtatgtca acacaacgaa cattgatggg ttccggcttg atgccgtcaa gcatattaag      720

ttcagttttt ttcctgattg gttgtcgtat gtgcgttctc agactggcaa gccgctattt      780

accgtcgggg aatattggag ctatgacatc aacaagttgc acaattacat tacgaaaaca      840

aacggaacga tgtctttgtt tgatgccccg ttacacaaca aattttatac cgcttccaaa      900

tcagggggcg catttgatat gcgcacgtta atgaccaata ctctcatgaa agatcaaccg      960

acattggccg tcaccttcgt tgataatcat gacaccgaac ccggccaagc gcttcagtca     1020

tgggtcgacc catggttcaa accgttggct tacgccttta ttctaactcg gcaggaagga     1080

tacccgtgcg tcttttatgg tgactattat ggcattccac aatataacat tccttcgctg     1140

aaaagcaaaa tcgatccgct cctcatcgcg cgcagggatt atgcttacgg aacgcaacat     1200

gattatcttg atcactccga catcatcggg tggacaaggg aaggggtcac tgaaaaacca     1260

ggatccgggc tggccgcact gatcaccgat gggccgggag gaagcaaatg gatgtacgtt     1320

ggcaaacaac acgctggaaa agtgttctat gaccttaccg gcaaccggag tgacaccgtc     1380

accatcaaca gtgatggatg gggggaattc aaagtcaatg gcggttcggt ttcggtttgg     1440

gttcctagaa aaacgaccta a                                               1461


<210>  71
<211>  34
<212>  DNA
<213>  Bacillus licheniformis

<400>  71
cggatttcct gaaggaaatc cgttttttta tttt                                   34


<210>  72
<211>  1809
<212>  DNA
<213>  Bacillus licheniformis

<400>  72
taacatctct cactgctgtg tgattttact cacggcattt ggaacgccgg ctctcaacaa       60

actttctgta gtgaaaatca tgaaccaaac ggatcgtcgg cctgattaac agctgaaagc      120

tgccgatcac aaacatccat agtcccgccg gcttcagttc ctcggagaaa aagcagaagc      180

tcccgacaag gaataaaagg ccgatgagaa aatcgtttaa tgtatgtaga actttgtatc      240

tttttttgaa aaagagttca tatcgattgt tattgttttg cggcattgct tgatcactcc      300

aatcctttta tttaccctgc cggaagccgg agtgaaacgc cggtatacat aggatttatg      360

aattaggaaa acatatgggg aaataaacca tccaggagtg aaaaatatgc ggttattcat      420

atgtgcatcg tgcctgttcg gcttgattgt tccgtcattt gaaacgaaag cgctgacgtt      480

tgaagaattg ccggttaaac aagcttcaaa acaatgggaa gttcaaatcg gtaaagccga      540

agccggaaac ggaatggcga aaccggaaaa aggagcgttt catacttatg ctgtcgaaat      600

caaaaacatt ggacacgatg tggcttcggc ggaaattttt gtctatcgga acgagcctaa      660

ttcttcaacg aaattttcgc tttggaacat tcctcacgaa aatccggttt ctttagccaa      720

aagcttaaat cacggaagct ctgtcaagca ccgcaatctg cttatggcag agaatgcgac      780

cgaattggaa gtggacatga tttggacgga aaaaggaagc gaaggcagac ttttaaagga      840

aacgttcatt ttcaagggag atgaatcatg aagaaaaaat ggccgttcat cgtcaacggt      900

ctttttttaa tgacttaggc agccgatcgt tcggccatac gatatcgaag cgacctcgaa      960

ccagcagagc tcgtcacaaa acatttgcat ttaaagaaaa atacaggatg ttttcaccaa     1020

tatttttctc aatgatgata cactattgac aagctgctac tttgggaggg tgtttccata     1080

gatgccgatg aagcaaaaac accaaatgtg tcatgagagc tctctctaat cgatataaaa     1140

gtagggtgaa ccggggttgt caatctgtaa aagatctttt tttatcccgt gatacgcttt     1200

tggaattctg aatcttcaag aaagtcccca gccttttgct gatcaatcga gaacaaagga     1260

tgatacatat gaaaagaata gataaaatct accatcagct gctggataat tttcgcgaaa     1320

agaatatcaa tcagctttta aagatacaag ggaattcggc taaagaaatc gccgggcagc     1380

tgcaaatgga gcgttccaat gtcagctttg aattaaacaa tctggttcgg gccaaaaagg     1440

tgatcaagat taaaacgttc cccgtccgct acatcccggt ggaaattgtt gaaaacgtct     1500

tgaacatcaa atggaattca gagttgatgg aggttgaaga actgaggcgg ctggctgacg     1560

gccaaaaaaa gccggcgcgc aatatatccg ccgatcccct cgagctcatg atcggggcta     1620

aagggagctt gaaaaaggca atttctcagg cgaaagcggc agtcttttat cctccgcacg     1680

gcttgcatat gctgctgctc gggccgacgg gttcggggaa atcgctgttt gcgaatcgga     1740

tctaccagtt cgccgtttat tctgacatat tgaagcccga ttccccgttc atcacattca     1800

actgtgcag                                                             1809


<210>  73
<211>  486
<212>  PRT
<213>  G. stearothermophilus

<400>  73

Ala Ala Pro Phe Asn Gly Thr Met Met Gln Tyr Phe Glu Trp Tyr Leu 
1               5                   10                  15      


Pro Asp Asp Gly Thr Leu Trp Thr Lys Val Ala Asn Glu Ala Asn Asn 
            20                  25                  30          


Leu Ser Ser Leu Gly Ile Thr Ala Leu Trp Leu Pro Pro Ala Tyr Lys 
        35                  40                  45              


Gly Thr Ser Arg Ser Asp Val Gly Tyr Gly Val Tyr Asp Leu Tyr Asp 
    50                  55                  60                  


Leu Gly Glu Phe Asn Gln Lys Gly Thr Val Arg Thr Lys Tyr Gly Thr 
65                  70                  75                  80  


Lys Ala Gln Tyr Leu Gln Ala Ile Gln Ala Ala His Ala Ala Gly Met 
                85                  90                  95      


Gln Val Tyr Ala Asp Val Val Phe Asp His Lys Gly Gly Ala Asp Gly 
            100                 105                 110         


Thr Glu Trp Val Asp Ala Val Glu Val Asn Pro Ser Asp Arg Asn Gln 
        115                 120                 125             


Glu Ile Ser Gly Thr Tyr Gln Ile Gln Ala Trp Thr Lys Phe Asp Phe 
    130                 135                 140                 


Pro Gly Arg Gly Asn Thr Tyr Ser Ser Phe Lys Trp Arg Trp Tyr His 
145                 150                 155                 160 


Phe Asp Gly Val Asp Trp Asp Glu Ser Arg Lys Leu Ser Arg Ile Tyr 
                165                 170                 175     


Lys Phe Arg Gly Ile Gly Lys Ala Trp Asp Trp Pro Val Asp Thr Glu 
            180                 185                 190         


Asn Gly Asn Tyr Asp Tyr Leu Met Tyr Ala Asp Leu Asp Met Asp His 
        195                 200                 205             


Pro Glu Val Val Thr Glu Leu Lys Asn Trp Gly Lys Trp Tyr Val Asn 
    210                 215                 220                 


Thr Thr Asn Ile Asp Gly Phe Arg Leu Asp Ala Val Lys His Ile Lys 
225                 230                 235                 240 


Phe Ser Phe Phe Pro Asp Trp Leu Ser Tyr Val Arg Ser Gln Thr Gly 
                245                 250                 255     


Lys Pro Leu Phe Thr Val Gly Glu Tyr Trp Ser Tyr Asp Ile Asn Lys 
            260                 265                 270         


Leu His Asn Tyr Ile Thr Lys Thr Asn Gly Thr Met Ser Leu Phe Asp 
        275                 280                 285             


Ala Pro Leu His Asn Lys Phe Tyr Thr Ala Ser Lys Ser Gly Gly Ala 
    290                 295                 300                 


Phe Asp Met Arg Thr Leu Met Thr Asn Thr Leu Met Lys Asp Gln Pro 
305                 310                 315                 320 


Thr Leu Ala Val Thr Phe Val Asp Asn His Asp Thr Glu Pro Gly Gln 
                325                 330                 335     


Ala Leu Gln Ser Trp Val Asp Pro Trp Phe Lys Pro Leu Ala Tyr Ala 
            340                 345                 350         


Phe Ile Leu Thr Arg Gln Glu Gly Tyr Pro Cys Val Phe Tyr Gly Asp 
        355                 360                 365             


Tyr Tyr Gly Ile Pro Gln Tyr Asn Ile Pro Ser Leu Lys Ser Lys Ile 
    370                 375                 380                 


Asp Pro Leu Leu Ile Ala Arg Arg Asp Tyr Ala Tyr Gly Thr Gln His 
385                 390                 395                 400 


Asp Tyr Leu Asp His Ser Asp Ile Ile Gly Trp Thr Arg Glu Gly Val 
                405                 410                 415     


Thr Glu Lys Pro Gly Ser Gly Leu Ala Ala Leu Ile Thr Asp Gly Pro 
            420                 425                 430         


Gly Gly Ser Lys Trp Met Tyr Val Gly Lys Gln His Ala Gly Lys Val 
        435                 440                 445             


Phe Tyr Asp Leu Thr Gly Asn Arg Ser Asp Thr Val Thr Ile Asn Ser 
    450                 455                 460                 


Asp Gly Trp Gly Glu Phe Lys Val Asn Gly Gly Ser Val Ser Val Trp 
465                 470                 475                 480 


Val Pro Arg Lys Thr Thr 
                485     


<210>  74
<211>  1967
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  catH locus WT construct

<400>  74
tgtgtgacgg ctatcatgcc ggcgctttta taaacgagtt ggaacggctt gccgccgatt       60

gtgaggagtg gcttgtgtga cagaggaaag gccgatatga ttcggccttt tttatatgta      120

cttcttagcg ggtctcttaa cccccctcga ggtcgctgat aaacagctga catcaatatc      180

ctattttttc aaaaaatatt ttaaaaagtt gttgacttaa aagaagctaa atgttatagt      240

aataaaacag aatagtcttt taagtaagtc tactctgaat ttttttaaaa ggagagggta      300

aagaatgaaa caacaaaaac ggctttacgc ccgattgctg acgctgttat ttgcgctcat      360

cttcttgctg cctcattctg cagctagcgc agccgcaccg tttaacggta ccatgatgca      420

gtattttgaa tggtacttgc cggatgatgg cacgttatgg accaaagtgg ccaatgaagc      480

caacaactta tccagccttg gcatcaccgc tctttggctg ccgcccgctt acaaaggaac      540

aagccgcagc gacgtagggt acggagtata cgacttgtat gacctcggcg aattcaatca      600

aaaagggacc gtccgcacaa aatatggaac aaaagctcaa tatcttcaag ccattcaagc      660

cgcccacgcc gctggaatgc aagtgtacgc cgatgtcgtg ttcgaccata aaggcggcgc      720

tgacggcacg gaatgggtgg acgccgtcga agtcaatccg tccgaccgca accaagaaat      780

ctcgggcacc tatcaaatcc aagcatggac gaaatttgat tttcccgggc ggggcaacac      840

ctactccagc tttaagtggc gctggtacca ttttgacggc gttgattggg acgaaagccg      900

aaaattaagc cgcatttaca aattcagggg catcggcaaa gcgtgggatt ggccggtaga      960

cacagaaaac ggaaactatg actacttaat gtatgccgac cttgatatgg atcatcccga     1020

agtcgtgacc gagctgaaaa actgggggaa atggtatgtc aacacaacga acattgatgg     1080

gttccggctt gatgccgtca agcatattaa gttcagtttt tttcctgatt ggttgtcgta     1140

tgtgcgttct cagactggca agccgctatt taccgtcggg gaatattgga gctatgacat     1200

caacaagttg cacaattaca ttacgaaaac aaacggaacg atgtctttgt ttgatgcccc     1260

gttacacaac aaattttata ccgcttccaa atcagggggc gcatttgata tgcgcacgtt     1320

aatgaccaat actctcatga aagatcaacc gacattggcc gtcaccttcg ttgataatca     1380

tgacaccgaa cccggccaag cgcttcagtc atgggtcgac ccatggttca aaccgttggc     1440

ttacgccttt attctaactc ggcaggaagg atacccgtgc gtcttttatg gtgactatta     1500

tggcattcca caatataaca ttccttcgct gaaaagcaaa atcgatccgc tcctcatcgc     1560

gcgcagggat tatgcttacg gaacgcaaca tgattatctt gatcactccg acatcatcgg     1620

gtggacaagg gaaggggtca ctgaaaaacc aggatccggg ctggccgcac tgatcaccga     1680

tgggccggga ggaagcaaat ggatgtacgt tggcaaacaa cacgctggaa aagtgttcta     1740

tgaccttacc ggcaaccgga gtgacaccgt caccatcaac agtgatggat ggggggaatt     1800

caaagtcaat ggcggttcgg tttcggtttg ggttcctaga aaaacgacct aaaagcttct     1860

cgaggttaac agaggacgga tttcctgaag gaaatccgtt tttttatttt taacatctct     1920

cactgctgtg tgattttact cacggcattt ggaacgccgg ctctcaa                   1967


<210>  75
<211>  1966
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  catH locus mod 5'-UTR construct

<400>  75
tgtgtgacgg ctatcatgcc ggcgctttta taaacgagtt ggaacggctt gccgccgatt       60

gtgaggagtg gcttgtgtga cagaggaaag gccgatatga ttcggccttt tttatatgta      120

cttcttagcg ggtctcttaa cccccctcga ggtcgctgat aaacagctga catcaatatc      180

ctattttttc aaaaaatatt ttaaaaagtt gttgacttaa aagaagctaa atgttatagt      240

aataaaacag aatagtcttt taagtaagtc tactctgaat ttttttaaaa ggagagggta      300

aagatgaaac aacaaaaacg gctttacgcc cgattgctga cgctgttatt tgcgctcatc      360

ttcttgctgc ctcattctgc agctagcgca gccgcaccgt ttaacggtac catgatgcag      420

tattttgaat ggtacttgcc ggatgatggc acgttatgga ccaaagtggc caatgaagcc      480

aacaacttat ccagccttgg catcaccgct ctttggctgc cgcccgctta caaaggaaca      540

agccgcagcg acgtagggta cggagtatac gacttgtatg acctcggcga attcaatcaa      600

aaagggaccg tccgcacaaa atatggaaca aaagctcaat atcttcaagc cattcaagcc      660

gcccacgccg ctggaatgca agtgtacgcc gatgtcgtgt tcgaccataa aggcggcgct      720

gacggcacgg aatgggtgga cgccgtcgaa gtcaatccgt ccgaccgcaa ccaagaaatc      780

tcgggcacct atcaaatcca agcatggacg aaatttgatt ttcccgggcg gggcaacacc      840

tactccagct ttaagtggcg ctggtaccat tttgacggcg ttgattggga cgaaagccga      900

aaattaagcc gcatttacaa attcaggggc atcggcaaag cgtgggattg gccggtagac      960

acagaaaacg gaaactatga ctacttaatg tatgccgacc ttgatatgga tcatcccgaa     1020

gtcgtgaccg agctgaaaaa ctgggggaaa tggtatgtca acacaacgaa cattgatggg     1080

ttccggcttg atgccgtcaa gcatattaag ttcagttttt ttcctgattg gttgtcgtat     1140

gtgcgttctc agactggcaa gccgctattt accgtcgggg aatattggag ctatgacatc     1200

aacaagttgc acaattacat tacgaaaaca aacggaacga tgtctttgtt tgatgccccg     1260

ttacacaaca aattttatac cgcttccaaa tcagggggcg catttgatat gcgcacgtta     1320

atgaccaata ctctcatgaa agatcaaccg acattggccg tcaccttcgt tgataatcat     1380

gacaccgaac ccggccaagc gcttcagtca tgggtcgacc catggttcaa accgttggct     1440

tacgccttta ttctaactcg gcaggaagga tacccgtgcg tcttttatgg tgactattat     1500

ggcattccac aatataacat tccttcgctg aaaagcaaaa tcgatccgct cctcatcgcg     1560

cgcagggatt atgcttacgg aacgcaacat gattatcttg atcactccga catcatcggg     1620

tggacaaggg aaggggtcac tgaaaaacca ggatccgggc tggccgcact gatcaccgat     1680

gggccgggag gaagcaaatg gatgtacgtt ggcaaacaac acgctggaaa agtgttctat     1740

gaccttaccg gcaaccggag tgacaccgtc accatcaaca gtgatggatg gggggaattc     1800

aaagtcaatg gcggttcggt ttcggtttgg gttcctagaa aaacgaccta aaagcttctc     1860

gaggttaaca gaggacggat ttcctgaagg aaatccgttt ttttattttt aacatctctc     1920

actgctgtgt gattttactc acggcatttg gaacgccggc tctcaa                    1966


<210>  76
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  catH locus forward primer

<400>  76
tgtgtgacgg ctatcatgcc                                                   20


<210>  77
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  catH locus reverse primer

<400>  77
ttgagagccg gcgttcc                                                      17


<210>  78
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  catH forward sequencing primer 1

<400>  78
aacgagttgg aacggcttgc                                                   20


<210>  79
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  catH forward sequencing primer 2

<400>  79
ggcaacacct actccagctt                                                   20


<210>  80
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  catH forward sequencing primer 3

<400>  80
gatcactccg acatcatcgg                                                   20


<210>  81
<211>  1205
<212>  DNA
<213>  Bacillus licheniformis

<400>  81
catcaatctt tttccagaca gtgatcaaaa ttcgacattt ttcatcaaaa acgtcaaaat       60

aaattgacgc gctttcatga gttttgtacg atatgagaga ttgatgacaa aaaggagctg      120

aggatcgtga acagatcctt taccgttgaa aaggtactca acaacaacgt tttaatcgct      180

ctccatgatg attacagaga agttgtcttg attggcaaag gaatcggttt tggtaaaaag      240

cgcggagatc ttatcgaaca tgagaactac gaaaaaatgt ttatcctgga aaatgataag      300

gaacaatcgc agtataagaa gctcctcact tatgtcgatg aaaaaatggt tgatatcgcc      360

aatgatgtca tctaccatat cgcgcaaaaa atcggccagc cgctgaacga acacattcat      420

gtcgccctga cggaccatat cgcatttgca gttaagcgtc tagaaaaggg atttgatatg      480

aaaaatccgt ttttgcttga gacggaatcg ctttatccga aggaatacga agtcgccaag      540

gaagccgtcg atatgattaa tgaaaaatcc gacattcagc tgcctgaagg tgaaatcggg      600

ttcatcgcgc ttcatatcca cagtgcgatg acaaaccgcc cgctttctga agtcaatcag      660

cattcacaac tgatctccag gcttgtccag gtcatcgaag attcattcca gatgcaagtc      720

aacagggaaa gcgtgaacta tttgcggctg atcaggcact tgcgctttac gattgacagg      780

ataaaacggg acgagccgat tcaggaaccg gaaaaattaa tgttgttgtt gaaaacggaa      840

tatccgctgt gttacaatac tgcttggaag atgatcaaga tcttgcagca agcgctcaag      900

aaaccggttc atgaggcaga agccgtttat ttgacattgc atttgtaccg tttgactaat      960

aaaatttcat aacgccttta taacgtgtta ctgattcgat caggcatgag tgaaaaaagg     1020

ggagaaatga agacgctgtc ttttgtttct tagtgtaccc gctttcacca tgcctttttt     1080

gtcgttctaa aggcgaaatg taaacggttt atttagatga aaaagcagtc ttttgaacga     1140

agttgtcatg aatttttgct gtaaggtcaa actattctta gagggggtta aaaaatgttt     1200

aaatc                                                                 1205


<210>  82
<211>  281
<212>  PRT
<213>  Bacillus licheniformis

<400>  82

Val Asn Arg Ser Phe Thr Val Glu Lys Val Leu Asn Asn Asn Val Leu 
1               5                   10                  15      


Ile Ala Leu His Asp Asp Tyr Arg Glu Val Val Leu Ile Gly Lys Gly 
            20                  25                  30          


Ile Gly Phe Gly Lys Lys Arg Gly Asp Leu Ile Glu His Glu Asn Tyr 
        35                  40                  45              


Glu Lys Met Phe Ile Leu Glu Asn Asp Lys Glu Gln Ser Gln Tyr Lys 
    50                  55                  60                  


Lys Leu Leu Thr Tyr Val Asp Glu Lys Met Val Asp Ile Ala Asn Asp 
65                  70                  75                  80  


Val Ile Tyr His Ile Ala Gln Lys Ile Gly Gln Pro Leu Asn Glu His 
                85                  90                  95      


Ile His Val Ala Leu Thr Asp His Ile Ala Phe Ala Val Lys Arg Leu 
            100                 105                 110         


Glu Lys Gly Phe Asp Met Lys Asn Pro Phe Leu Leu Glu Thr Glu Ser 
        115                 120                 125             


Leu Tyr Pro Lys Glu Tyr Glu Val Ala Lys Glu Ala Val Asp Met Ile 
    130                 135                 140                 


Asn Glu Lys Ser Asp Ile Gln Leu Pro Glu Gly Glu Ile Gly Phe Ile 
145                 150                 155                 160 


Ala Leu His Ile His Ser Ala Met Thr Asn Arg Pro Leu Ser Glu Val 
                165                 170                 175     


Asn Gln His Ser Gln Leu Ile Ser Arg Leu Val Gln Val Ile Glu Asp 
            180                 185                 190         


Ser Phe Gln Met Gln Val Asn Arg Glu Ser Val Asn Tyr Leu Arg Leu 
        195                 200                 205             


Ile Arg His Leu Arg Phe Thr Ile Asp Arg Ile Lys Arg Asp Glu Pro 
    210                 215                 220                 


Ile Gln Glu Pro Glu Lys Leu Met Leu Leu Leu Lys Thr Glu Tyr Pro 
225                 230                 235                 240 


Leu Cys Tyr Asn Thr Ala Trp Lys Met Ile Lys Ile Leu Gln Gln Ala 
                245                 250                 255     


Leu Lys Lys Pro Val His Glu Ala Glu Ala Val Tyr Leu Thr Leu His 
            260                 265                 270         


Leu Tyr Arg Leu Thr Asn Lys Ile Ser 
        275                 280     


<210>  83
<211>  140
<212>  PRT
<213>  Bacillus licheniformis

<400>  83

Met Ala Met Thr Arg Phe Gly Glu Arg Leu Lys Glu Leu Arg Glu Gln 
1               5                   10                  15      


Arg Ser Leu Ser Val Asn Gln Leu Ala Met Tyr Ala Gly Val Ser Ala 
            20                  25                  30          


Ala Ala Ile Ser Arg Ala Ala Ala Ile Ser Arg Ile Glu Asn Gly His 
        35                  40                  45              


Arg Gly Val Pro Lys Pro Ala Thr Ile Arg Lys Leu Ala Glu Ala Leu 
    50                  55                  60                  


Lys Met Pro Tyr Glu Gln Leu Met Asp Ile Ala Gly Tyr Met Arg Ala 
65                  70                  75                  80  


Asp Glu Ile Arg Glu Gln Pro Arg Gly Tyr Val Thr Met Gln Glu Ile 
                85                  90                  95      


Ala Ala Lys His Gly Val Glu Asp Leu Trp Leu Phe Lys Pro Glu Lys 
            100                 105                 110         


Trp Asp Cys Leu Ser Arg Glu Asp Leu Leu Asn Leu Glu Gln Tyr Phe 
        115                 120                 125             


His Phe Leu Val Asn Glu Ala Lys Lys Arg Gln Ser 
    130                 135                 140 


<210>  84
<211>  134
<212>  PRT
<213>  Bacillus licheniformis

<400>  84

Met Ala Met Thr Arg Phe Gly Glu Arg Leu Lys Glu Leu Arg Glu Gln 
1               5                   10                  15      


Arg Ser Leu Ser Val Asn Gln Leu Ala Met Tyr Ala Gly Val Ser Ala 
            20                  25                  30          


Ala Ala Ile Ser Arg Ile Glu Asn Gly His Arg Gly Val Pro Lys Pro 
        35                  40                  45              


Ala Thr Ile Arg Lys Leu Ala Glu Ala Leu Lys Met Pro Tyr Glu Gln 
    50                  55                  60                  


Leu Met Asp Ile Ala Gly Tyr Met Arg Ala Asp Glu Ile Arg Glu Gln 
65                  70                  75                  80  


Pro Arg Gly Tyr Val Thr Met Gln Glu Ile Ala Ala Lys His Gly Val 
                85                  90                  95      


Glu Asp Leu Trp Leu Phe Lys Pro Glu Lys Trp Asp Cys Leu Ser Arg 
            100                 105                 110         


Glu Asp Leu Leu Asn Leu Glu Gln Tyr Phe His Phe Leu Val Asn Glu 
        115                 120                 125             


Ala Lys Lys Arg Gln Ser 
    130                 


