﻿                         SEQUENCE LISTING

<110>  天津科技大学
 
<120>  高产尿苷的基因工程菌及其构建方法与应用

<160>  3     

<170>  PatentIn version 3.5

<210>  1
<211>  9492
<212>  DNA
<213>  B. subtilis

<400>  1
atgaagcatt taacgacgat gagtgaactt agcactgagg aaatcaaaga tttgcttcaa       60

acagcacaag agctcaaaag cggaaaaaca gacaatcagc ttacaggaaa gtttgcagca      120

aacctgtttt tcgaaccgag cacgagaacg cggttcagct ttgaggtcgc agaaaaaaag      180

ctgggcatga atgtgcttaa ccttgatgga acaagcacaa gcgtgcaaaa aggcgaaacc      240

ttatatgaca cgatccggac gcttgaatca atcggtgtgg acgtctgcgt catcaggcac      300

agtgaggatg agtattatga agagcttgtc agccaggtga acattccgat tctgaatgcg      360

ggagacgggt gcggccagca tccaacacaa tcactgcttg atttaatgac gatttatgaa      420

gagttcaata cgtttaaagg gcttaccgtc tccattcacg gcgacatcaa gcatagcaga      480

gtggcaaggt caaatgcgga agtgttgaca agattgggtg cccgggtcct attttccggc      540

ccttcggaat ggcaggatga agaaaataca ttcggcacgt atgtctcaat ggatgaagca      600

gttgagtctt ccgatgttgt catgctgctg cgcattcaaa atgaacgaca tcagtccgct      660

gtcagtcagg aaggctattt aaacaaatac ggcttgaccg tagaacgggc tgagcgtatg      720

aagcggcatg cgatcatcat gcatcctgct ccggtaaaca gaggagtgga gattgatgac      780

agcttagtag aaagcgaaaa atcaagaatc ttcaagcaaa tgaaaaatgg cgtatttatc      840

agaatggcag tgatacagcg tgccttacaa accaatgtga aaagaggaga agcagcgtat      900

gtcatatctc attaaaaacg gctggatact aaacgaaaat ggtgaaaaaa cacaagcgga      960

tatccgagtg actggagaaa ccatcaccgc aatcggcaag cttgatgcaa cggataatga     1020

aacggtaatt gatgcaaaag gtttgctcgt ttcacctggg tttgttgatc tccacgtgca     1080

tttcagagag ccgggcggag agaaaaaaga aactattgaa accggggcaa aagcagcggc     1140

gcgcggcggc tatactacag tagcagcaat gccgaatacg cggccggttc ctgatacaaa     1200

ggagcagatg gaatgggtgc aaaacagaat taaagaaaca tcatgcgtaa gagttcttcc     1260

atatgcatcc attacgatca gacaaatcgg cgatgaaatg acaaactttg aagcgttaaa     1320

agaagccggg gcatttgctt ttacagatga cggcgttggt atacagacag caggaatgat     1380

gtatgaagcg atgaaacggg cagccgcaat tgacaaagcg attgttgcac attgcgagga     1440

caactcctta atttacggag ggagcgtaca tgaggggaca ttctccaaag cgaacgggct     1500

aaacggcatt ccttctgtgt gtgaatcggt tcatattgct cgcgatgtgc tgctggctga     1560

ggcggcaaac tgccattatc atgtatgcca tatcagcaca aaagaatctg tcagagttgt     1620

acgcgatgcg aaaaaagcgg gaatcagagt gacagcagaa gtatcgccgc atcatttgct     1680

gctttgtgat gaggacatcc cggggctgga cacaaactat aaaatgaatc ctccgctccg     1740

cagcccagaa gacagagctg ctttaattga aggtctttta gacggaacaa ttgattttat     1800

cgcaacagac catgcaccgc atacggaaga agagaagaac acagaaatga agctggcgcc     1860

attcggaatt gtcggcttag aaacagcatt cccgcttctt tacacacact ttgtcaaaaa     1920

tggcagctgg tcactgaagc agctgattga ctacatgaca atcaagccat gcgaagcgtt     1980

cggtctccca tatggaacat tacaaacggg gcaagctgcg gacattacgt taatcgattt     2040

agaaaaagaa gcagttatag acaaagagac atttttatca aaaggaaaaa atacaccatt     2100

caacggcatc agttgcaccg gctggccggt cgctacaatt gcggcaggga agcttgctta     2160

tgaagagggg agacttgtca aatgaagaga cgattagtac tggaaaacgg agcggtattc     2220

gagggagaag ctttcggaag cttagaacac aacatgggag aagtcgtttt taatactggg     2280

atgacaggct atcaggaaat tttatctgat ccttcttact gcggacagat cgtaacatta     2340

acatacccgc ttatcggaaa ttacggcatt aaccgtgatg attttgaatc cattacccct     2400

tttgtcaaag ggctgatcat caaagaatta tgtgagctgc cttccaactg gcgttcagca     2460

tacaccttag acgagtattt aaaaatgaaa aacattcccg gactccaggg aattgataca     2520

aggaagctga caagaatgat ccgcacggca ggcgcgctaa aaggaacatt cgcttcatct     2580

gatgaagata tcgaagcagt gctgaaaaga ctgaacgaaa cggaattgcc aagaaatcaa     2640

gtatcccaag tatcagcaaa aacagcatat ccgagcccgg gaagaggcaa acgcattgtc     2700

ttggttgact tcggcatgaa acacgggatt ctaagagagc tgaacaaacg gaaatgtgac     2760

gtcatcgttg tgccttacaa cattacagcg gaagaggtgc ttcagctgaa accggacggt     2820

atcatgcttt ctaacggacc tggagacccg aaggatgtgc ctgaagcgat tgaaatgatt     2880

aaaggtgttc ttggaaaagt gccattattc ggaatatgtc tcggccacca attattcgcg     2940

ctggcgtgcg gggcgaatac tgaaaaaatg aaattcggcc acaggggctc aaaccacccg     3000

gtaaaagagc tggctacagg aaaagttgcc ttaacatctc aaaaccatgg atatacagtt     3060

tcgtctatca gtaaaacaga actggaagtg acgcatatcg caattaacga cgatacgatt     3120

gaagggctga agcataaaac attgccggca tttacggttc aatatcatcc cgaagcctca     3180

cctggtcctg aggatgccaa ccatctattt gacagattca tcgaaatgat cgaaacaaca     3240

gagaaagaag gggaagcggt atgccaaaac gcgtagacat taacaaaatt ttagtaatcg     3300

gatctggacc gatcatcatc ggccaagcag cagaatttga ctatgcggga acacaagcct     3360

gtcttgcttt gaaagaagaa ggctatgaag tcatccttgt caactcaaac cctgcaacga     3420

tcatgacaga tacagaaatg gctgaccggg tttacatcga accgctcaca cctgaattcc     3480

tgacacgaat catcagaaaa gagcgcccgg atgccattct tcctacactc ggaggccaaa     3540

ccggtttgaa tcttgcggtt gagctttctg aaagaggcgt tttggcagaa tgcggcgtcg     3600

aagtgcttgg cacgaaactg tctgcgattc agcaagctga agaccgtgac ttgttcagaa     3660

cattaatgaa tgaactgaat gaaccggtgc ctgaaagtga gattatccac tcccttgaag     3720

aagcagaaaa attcgtcagt caaattggat tccctgtcat tgtccgcccg gcatatacat     3780

taggcggaac aggcggaggc atctgctcga atgaaacaga gctaaaagaa atcgttgaga     3840

acggcttgaa attaagcccg gtacaccaat gtctgcttga aaaaagcatc gccggctata     3900

aagaaatcga gtatgaagtc atgagagaca gccaggatca cgccattgtc gtttgtaaca     3960

tggaaaacat tgatccagtt ggaatccata ctggagacag tattgttgtc gcgccgagcc     4020

aaacgctcag cgatcgcgaa tatcagctct tgcggaatgt atcgttaaaa ctgattcgcg     4080

cgcttgggat cgaaggcgga tgtaatgtcc agctcgcctt agatccagac agcttccaat     4140

attacattat tgaagtaaat ccgcgtgtca gccgttcatc tgcccttgca tcaaaagcaa     4200

cggggtaccc gattgcaaag ctcgctgcta aaattgcagt cggactttca ttagatgaaa     4260

tgatgaaccc ggtgacagga aaaacatatg cagcatttga acctgctctt gactatgtcg     4320

tatccaaaat tccgcgctgg ccgtttgata agtttgaatc agcaaacaga aagcttggca     4380

cgcaaatgaa agcgacaggt gaggtcatgg caatcggccg cacgcttgaa gagtcattgc     4440

tgaaggcagt gcgatcactg gaagcggatg tgtatcatct tgaattgaag gacgccgctg     4500

acatttcaga tgagcttctt gaaaagcgaa ttaaaaaggc cggtgatgaa cgcttattct     4560

acttagctga agcgtacaga agaggctaca cggtagaaga cctccatgaa ttttccgcta     4620

tcgatgtctt cttcttgcat aagctgttcg gaatcgtaca gtttgaaaaa gaattgaagg     4680

ccaatgcggg cgatacagat gtgctgagac gggcaaaaga actcggcttc tctgatcagt     4740

acatcagccg tgaatggaaa atgaaagaat ctgagcttta cagcttgaga aaacaagcgg     4800

ggattgcgcc ggtattcaaa atggtagata catgcgcggc ggaatttgag tcagaaacgc     4860

catacttcta tagcacatat gaagaagaaa atgaatctgt cgttacagat aagaaaagtg     4920

tgatggtgct tggttcgggt ccgattcgaa tcggtcaggg tgtcgagttc gactatgcga     4980

cggttcactc tgtatgggca attaaacaag caggctatga agccattatt gtcaacaaca     5040

acccggaaac cgtttcaaca gacttcagca tctcagacaa gctgtatttt gaaccgctta     5100

cgattgaaga tgtcatgcac atcattgacc tcgaacagcc aatgggcgtt gtcgtacaat     5160

ttggcggaca aactgcgatt aaccttgctg acgagctttc tgcacgcgga gtgaaaatcc     5220

ttggaacttc attagaagat ttagaccgtg ccgaagaccg ggataaattt gaacaagcgc     5280

ttggagaact tggtgttcct cagccgcttg gcaaaacagc gacatcagtt aatcaggcgg     5340

taagcatcgc aagtgatatc ggttatccgg tactggtacg cccttcctat gtacttggcg     5400

gccgggcgat ggagattgtt taccatgaag aggaactgct tcattacatg aaaaatgcag     5460

tcaaaatcaa tccacagcac cctgtattaa ttgatagata cttgaccgga aaagaaattg     5520

aagtcgatgc agtatccgac ggtgaaacag tcgtcattcc gggaattatg gagcacattg     5580

aacgtgcggg cgttcactcc ggagactcaa tcgctgttta tccgcctcag tctctcacag     5640

aggacattaa gaaaaaaatt gaacaataca cgatcgcatt ggctaaaggg ctgaatattg     5700

tcggtttgct caatattcaa ttcgtcttgt cgcaaggcga ggtgtacgtg ctagaagtga     5760

atccgagatc aagcagaacc gtaccgtttt taagcaaaat tacgggtatc ccaatggcga     5820

atctcgcaac aaaaatcatt cttggtcaaa agctggctgc gtttggctat acagagggcc     5880

ttcagcctga acagcaaggt gtatttgtaa aagcgccggt cttctccttt gccaagctga     5940

gaagagtgga tattacgtta gggcctgaaa tgaaatcaac aggtgaagtc atggggaaag     6000

attcgacact tgaaaaggcg ctctacaaag ccttgatcgc ttcaggtatt caaatcccga     6060

actacggttc cgtgctttta acagtagctg ataaggacaa agaagggctt gccattgcta     6120

agcggttcca cgcgatcggc tacaacattt tagcgacgga aggaacggca ggctacctga     6180

aagaagcttc cattccagcg aaggtcgtcg gaaaaatcgg tcaggatggc ccgaacttgc     6240

ttgatgtcat cagaaacgga gaagcgcagt ttgtcatcaa tacgctgaca aaaggaaagc     6300

agccggcaag agacggtttt agaatcagac gtgaatcagt agaaaatggt gttgcctgcc     6360

taacatcttt agatacggca gaggcgatat tgcgagtgct ggaaagcatg acattccgtg     6420

ctgatcaaat gccggcagtc aacacaaatc aggaggcggc agtcactata tgaaaaaagc     6480

gtatttgaca gtatgttcta accagcaaat tgcagaccgg gtgtttcaaa tggttctgaa     6540

aggggagctt gtccaagggt ttacaacccc tggacagttc cttcatctta aagtgagcga     6600

agcggttacg cctcttttga gaaggccgat cagcatcgca gacgtcaact ttgaaaaaaa     6660

tgaagtcacc atcatttatc gggtagatgg ggaagggaca agactcttgt cactgaagca     6720

gcagggagaa cttgtggatg tcctcgggcc tttgggaaat ggctttcctg ttaatgaagt     6780

tcaacccgga aagacggctt tgctggtagg aggcggagta ggtgtgccgc ctctccaaga     6840

gctgtcgaaa cgcttgattg aaaaaggggt aaatgtcatc cacgttttag gattccaatc     6900

ggcaaaggat gttttttacg aggaagaatg ccggcagtac ggagacacgt atgtggcaac     6960

agctgacgga agctacgggg aaaccggatt tgtcacagat gtgattaaac ggaaaaagct     7020

agagtttgat atcctcctca gctgcgggcc gacaccgatg ctcaaggcgt taaaacagga     7080

atatgcccat aaagaagtat atctgtccat ggaggaacga atgggctgcg gaatcggcgc     7140

atgcttcgcg tgtgtgtgcc atacaaacga aagtgagaca tcctatgtaa aagtatgtct     7200

cgacgggcct gtatttaaag ctcaggaggt ggcgctgtaa tgctagaggt gaaattgccg     7260

ggacttgatt tgaaaaaccc aatcattcct gcatcaggct gcttcggttt tggaaaagaa     7320

ttttcacgtt tttatgattt gtcttgtctt ggagctatca tgattaaggc tacgacaaag     7380

gagccgcgct ttgggaatcc gacgccgcgg gtagctgaga ctggtgctgg aatgctcaat     7440

gcgatcggtc tccaaaatcc ggggctggat agtgtgttgc atcatgagct gccgtggctt     7500

gagcagtttg atacaccgat cattgccaat gtcgcaggtt ctcaagtcga tgattatgtt     7560

gaagtcgcag aacatatcag caaagcgcct aatgttcatg ctcttgaatt gaatatttcc     7620

tgcccgaatg tgaaaacagg cggaatcgct tttggcacga atcctgaaat ggctgccgat     7680

ttgacaaaag cggtgaaaga ggtttcggat gtacccgttt atgtgaagct atccccgaac     7740

gtggctaata tcacagaaat tgcattagcg atcgaggaag cgggagcgga cggtcttacg     7800

atgatcaaca cactaatcgg catgagactc gatttaaaaa ccggcaaacc gatattagcg     7860

aataaaacag ggggactttc gggccctgct gtgaagccgg ttgccattcg catggtgtat     7920

gaagtcagcc agatggtcaa catcccgatt atcggaatgg gaggcgtgca aacggctgaa     7980

gatgccctgg aatttcttct cgcgggagca agcgcagtcg ctgtcggaac agcaaacttt     8040

gtgaatcctt ttgcatgtcc agagattatt gaacagctcc catctgtttt gctccaatac     8100

ggctatcaat caattgaaga atgcatcgga aggagctgga atcatgaaaa acaacctgcc     8160

catcatcgcg cttgattttg cgtcagctga agaaacactt gcgttcttag cgccttttca     8220

gcaagaaccg ttatttgtaa aggttgggat ggagcttttt tatcaagaag ggccatctat     8280

cgtgaaacaa ctaaaagaaa gaaactgcga gctattttta gatctaaagc ttcatgacat     8340

cccgactact gtaaacaaag cgatgaagcg ccttgccagt cttggagtag acctcgtcaa     8400

tgttcatgct gccgggggca aaaaaatgat gcaggcagct ctcgaaggct tagaagaagg     8460

tacgccggct ggaaaaaaac gtccgtcact tatcgcggta acccagctga caagcacatc     8520

tgaacaaatc atgaaagatg aactgctgat cgaaaagtct ctgattgata cggttgtgca     8580

ctacagcaaa caggcggaag aaagcggact ggatggagtg gtctgctctg ttcatgaagc     8640

aaaagccatt taccaagcgg tgtcgccttc atttctgact gtcactccgg ggatcagaat     8700

gtcagaggac gctgcgaatg accaagttcg cgtagcgacg cctgccattg caagagagaa     8760

aggttcatca gcgattgtag taggacgctc gattacaaaa gcggaagacc cggtaaaagc     8820

ctataaggct gtcagacttg aatgggaggg aatcaaatct tgaaacaaat catcgcaaaa     8880

catctattag acatccaagc tgtattttta cgcccgaacg agccgttcac atgggcaagc     8940

ggcattttat caccgatcta ctgtgacaac cgccttacgc tatctttccc agaggtcaga     9000

aacgatgttg cttcaggtat cagcaagctt gttaaagagc attttcctga agctgaaatg     9060

attgcgggaa cagcaactgc cggtattcct catgctgctc ttgcggcgga ccatttgaat     9120

cttccgatgt gttatgtgag gagcaagccg aaggcgcacg gaaaaggcaa tcagattgag     9180

ggagctgtgc aagaagggca aaaaacagtc gtcattgaag acttaatttc cacaggaggc     9240

agcgtgcttg aagcttgtgc agctttacaa gcggccggct gtgaagtgct tggtgtcgtc     9300

tcaatcttta cgtacggact tcctaaagcg gaggaagcct tcgcaaaggc agaactgcca     9360

tactactcat taaccgatta tgatacgctc acagaggtcg cgcttgaaaa cggaaatatt     9420

cattcagatg atctaaaaaa gctgcaaaca tggaaacgaa atcccgagtc aaaagattgg     9480

tttaaaaaat aa                                                         9492


<210>  2
<211>  74
<212>  DNA
<213>  E. coli W3110

<400>  2
ttgacaatta atcatccggc tcgtataatg tgtggaattg tgagcggata acaatttcac       60

acaggaaaca gacc                                                         74


<210>  3
<211>  948
<212>  DNA
<213>  E. coli W3110

<400>  3
gtgcctgata tgaagctttt tgctggtaac gccaccccgg aactagcaca acgtattgcc       60

aaccgcctgt acacttcact cggcgacgcc gctgtaggtc gctttagcga tggcgaagtc      120

agcgtacaaa ttaatgaaaa tgtacgcggt ggtgatattt tcatcatcca gtccacttgt      180

gcccctacta acgacaacct gatggaatta gtcgttatgg ttgatgccct gcgtcgtgct      240

tccgcaggtc gtatcaccgc tgttatcccc tactttggct atgcgcgcca ggaccgtcgc      300

gtccgttccg ctcgtgtacc aatcactgcg aaagtggttg cagacttcct ctccagcgtc      360

ggtgttgacc gtgtgctgac agtggatctg cacgctgaac agattcaggg tttcttcgac      420

gttccggttg ataacgtatt tggtagcccg atcctgctgg aagacatgct gcagctgaat      480

ctggataacc caattgtggt ttctccggac atcggcggcg ttgtgcgtgc ccgcgctatc      540

gctaagctgc tgaacgatac cgatatggca atcatcgaca aacgtcgtcc gcgtgcgaac      600

gtttcacagg tgatgcatat catcggtgac gttgcaggtc gtgactgcgt actggtcgat      660

gatatgatcg acactggcgg tacgctgtgt aaagctgctg aagctctgaa agaacgtggt      720

gctaaacgtg tatttgcgta cgcgactcac ccgatcttct ctggcaacgc ggcgaacaac      780

ctgcgtaact ctgtaattga tgaagtcgtt gtctgcgata ccattccgct gagcgatgaa      840

atcaaatcac tgccgaacgt gcgtactctg accctgtcag gtatgctggc cgaagcgatt      900

cgtcgtatca gcaacgaaga atcgatctct gccatgttcg aacactaa                   948


