
                                SEQUENCE LISTING

<110> Seattle Children's Hospital (dba Seattle Children's Research Institute)
      ENDOCYTE, INC.
      PURDUE RESEARCH FOUNDATION

<120> METHODS OF USE FOR CAR T CELLS

<130> SCRI.200WO

<150> 62/620,414          
<151> 2017-02-07  

<150> 62/620,706          
<151> 2018-01-23  

<150> 62/656,233          
<151> 2018-04-11  

<150> 62/724,171          
<151> 2018-08-29  

<150> 62/735,627          
<151> 2018-09-24  

<150> 62/736,727          
<151> 2018-09-26  

<160> 4

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 3198
<212> DNA
<213> Artificial Sequence

<220> 
<223> E2 anti-fluorescein antibody fragment CAR nucleic
      acid sequence

<400> 1
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccaagcg tgctgacaca gcctagctcc gtgtctgccg cccctggcca gaaagtgacc 120
atcagctgta gcggcagcac cagcaacatc ggcaacaact acgtgtcctg gtatcagcag 180
caccccggca aggcccccaa gctgatgatc tacgacgtgt ccaagcggcc cagcggcgtg 240
cccgatagat tttccggcag caagagcggc aacagcgcca gcctggatat cagcggcctg 300
cagtctgagg acgaggccga ctactattgc gccgcctggg acgatagcct gagcgagttc 360
ctgtttggca ccggcaccaa gctgacagtg ctgggcggag gcggaggatc tggcggcgga 420
ggaagtggcg gagggggatc tcaggtgcag ctggtggaaa gcggcggcaa cctggtgcag 480
cctggcggat ctctgagact gagctgtgcc gccagcggct tcaccttcgg cagcttcagc 540
atgagctggg tgcgccaggc tcctggggga ggactggaat gggtggcagg actgagcgcc 600
agaagcagcc tgacccacta cgccgatagc gtgaagggcc ggttcaccat cagccgggac 660
aacgccaaga acagcgtgta cctgcagatg aacagcctgc gggtggaaga taccgccgtg 720
tactactgcg ccagacggtc ctacgacagc agcggctact ggggccactt ctacagctac 780
atggacgtgt ggggccaggg caccctcgtg acagtgtctg agagcaagta cggaccgccc 840
tgcccccctt gccctgcccc cgagttcgac ggcggaccca gcgtgttcct gttccccccc 900
aagcccaagg acaccctgat gatcagccgg acccccgagg tgacctgcgt ggtggtggac 960
gtgagccagg aagatcccga ggtccagttc aattggtacg tggacggcgt ggaagtgcac 1020
aacgccaaga ccaagcccag agaggaacag ttccagagca cctaccgggt ggtgtctgtg 1080
ctgaccgtgc tgcaccagga ctggctgaac ggcaaagaat acaagtgcaa ggtgtccaac 1140
aagggcctgc ccagcagcat cgaaaagacc atcagcaagg ccaagggcca gcctcgcgag 1200
ccccaggtgt acaccctgcc tccctcccag gaagagatga ccaagaacca ggtgtccctg 1260
acctgcctgg tgaagggctt ctaccccagc gacatcgccg tggagtggga gagcaacggc 1320
cagcctgaga acaactacaa gaccacccct cccgtgctgg acagcgacgg cagcttcttc 1380
ctgtacagcc ggctgaccgt ggacaagagc cggtggcagg aaggcaacgt ctttagctgc 1440
agcgtgatgc acgaggccct gcacaaccac tacacccaga agagcctgag cctgtccctg 1500
ggcaagatgt tctgggtgct ggtggtggtg ggcggggtgc tggcctgcta cagcctgctg 1560
gtgacagtgg ccttcatcat cttttgggtg aaacggggca gaaagaaact cctgtatata 1620
ttcaaacaac catttatgag accagtacaa actactcaag aggaagatgg ctgtagctgc 1680
cgatttccag aagaagaaga aggaggatgt gaactgcggg tgaagttcag cagaagcgcc 1740
gacgcccctg cctaccagca gggccagaat cagctgtaca acgagctgaa cctgggcaga 1800
agggaagagt acgacgtcct ggataagcgg agaggccggg accctgagat gggcggcaag 1860
cctcggcgga agaaccccca ggaaggcctg tataacgaac tgcagaaaga caagatggcc 1920
gaggcctaca gcgagatcgg catgaagggc gagcggaggc ggggcaaggg ccacgacggc 1980
ctgtatcagg gcctgtccac cgccaccaag gatacctacg acgccctgca catgcaggcc 2040
ctgcccccaa ggctcgaggg cggcggagag ggcagaggaa gtcttctaac atgcggtgac 2100
gtggaggaga atcccggccc taggatgctt ctcctggtga caagccttct gctctgtgag 2160
ttaccacacc cagcattcct cctgatccca cgcaaagtgt gtaacggaat aggtattggt 2220
gaatttaaag actcactctc cataaatgct acgaatatta aacacttcaa aaactgcacc 2280
tccatcagtg gcgatctcca catcctgccg gtggcattta ggggtgactc cttcacacat 2340
actcctcctc tggatccaca ggaactggat attctgaaaa ccgtaaagga aatcacaggg 2400
tttttgctga ttcaggcttg gcctgaaaac aggacggacc tccatgcctt tgagaaccta 2460
gaaatcatac gcggcaggac caagcaacat ggtcagtttt ctcttgcagt cgtcagcctg 2520
aacataacat ccttgggatt acgctccctc aaggagataa gtgatggaga tgtgataatt 2580
tcaggaaaca aaaatttgtg ctatgcaaat acaataaact ggaaaaaact gtttgggacc 2640
tccggtcaga aaaccaaaat tataagcaac agaggtgaaa acagctgcaa ggccacaggc 2700
caggtctgcc atgccttgtg ctcccccgag ggctgctggg gcccggagcc cagggactgc 2760
gtctcttgcc ggaatgtcag ccgaggcagg gaatgcgtgg acaagtgcaa ccttctggag 2820
ggtgagccaa gggagtttgt ggagaactct gagtgcatac agtgccaccc agagtgcctg 2880
cctcaggcca tgaacatcac ctgcacagga cggggaccag acaactgtat ccagtgtgcc 2940
cactacattg acggccccca ctgcgtcaag acctgcccgg caggagtcat gggagaaaac 3000
aacaccctgg tctggaagta cgcagacgcc ggccatgtgt gccacctgtg ccatccaaac 3060
tgcacctacg gatgcactgg gccaggtctt gaaggctgtc caacgaatgg gcctaagatc 3120
ccgtccatcg ccactgggat ggtgggggcc ctcctcttgc tgctggtggt ggccctgggg 3180
atcggcctct tcatgtga                                               3198

<210> 2
<211> 1065
<212> PRT
<213> Artificial Sequence

<220> 
<223> E2 anti-fluorescein antibody fragment CAR amino
      acid sequence

<400> 2
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Ser Val Leu Thr Gln Pro Ser Ser Val Ser
            20                  25                  30          
Ala Ala Pro Gly Gln Lys Val Thr Ile Ser Cys Ser Gly Ser Thr Ser
        35                  40                  45              
Asn Ile Gly Asn Asn Tyr Val Ser Trp Tyr Gln Gln His Pro Gly Lys
    50                  55                  60                  
Ala Pro Lys Leu Met Ile Tyr Asp Val Ser Lys Arg Pro Ser Gly Val
65                  70                  75                  80  
Pro Asp Arg Phe Ser Gly Ser Lys Ser Gly Asn Ser Ala Ser Leu Asp
                85                  90                  95      
Ile Ser Gly Leu Gln Ser Glu Asp Glu Ala Asp Tyr Tyr Cys Ala Ala
            100                 105                 110         
Trp Asp Asp Ser Leu Ser Glu Phe Leu Phe Gly Thr Gly Thr Lys Leu
        115                 120                 125             
Thr Val Leu Gly Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly
    130                 135                 140                 
Gly Gly Ser Gln Val Gln Leu Val Glu Ser Gly Gly Asn Leu Val Gln
145                 150                 155                 160 
Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe
                165                 170                 175     
Gly Ser Phe Ser Met Ser Trp Val Arg Gln Ala Pro Gly Gly Gly Leu
            180                 185                 190         
Glu Trp Val Ala Gly Leu Ser Ala Arg Ser Ser Leu Thr His Tyr Ala
        195                 200                 205             
Asp Ser Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asn Ala Lys Asn
    210                 215                 220                 
Ser Val Tyr Leu Gln Met Asn Ser Leu Arg Val Glu Asp Thr Ala Val
225                 230                 235                 240 
Tyr Tyr Cys Ala Arg Arg Ser Tyr Asp Ser Ser Gly Tyr Trp Gly His
                245                 250                 255     
Phe Tyr Ser Tyr Met Asp Val Trp Gly Gln Gly Thr Leu Val Thr Val
            260                 265                 270         
Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro Ala Pro Glu
        275                 280                 285             
Phe Asp Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys Pro Lys Asp
    290                 295                 300                 
Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val Val Val Asp
305                 310                 315                 320 
Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr Val Asp Gly
                325                 330                 335     
Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu Gln Phe Gln
            340                 345                 350         
Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His Gln Asp Trp
        355                 360                 365             
Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys Gly Leu Pro
    370                 375                 380                 
Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln Pro Arg Glu
385                 390                 395                 400 
Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met Thr Lys Asn
                405                 410                 415     
Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro Ser Asp Ile
            420                 425                 430         
Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn Tyr Lys Thr
        435                 440                 445             
Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu Tyr Ser Arg
    450                 455                 460                 
Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val Phe Ser Cys
465                 470                 475                 480 
Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln Lys Ser Leu
                485                 490                 495     
Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val Val Gly Gly
            500                 505                 510         
Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe Ile Ile Phe
        515                 520                 525             
Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe Lys Gln Pro
    530                 535                 540                 
Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly Cys Ser Cys
545                 550                 555                 560 
Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg Val Lys Phe
                565                 570                 575     
Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln Asn Gln Leu
            580                 585                 590         
Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp Val Leu Asp
        595                 600                 605             
Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro Arg Arg Lys
    610                 615                 620                 
Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp Lys Met Ala
625                 630                 635                 640 
Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg Arg Gly Lys
                645                 650                 655     
Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr Lys Asp Thr
            660                 665                 670         
Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu Glu Gly Gly
        675                 680                 685             
Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val Glu Glu Asn
    690                 695                 700                 
Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu
705                 710                 715                 720 
Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val Cys Asn Gly
                725                 730                 735     
Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn Ala Thr Asn
            740                 745                 750         
Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp Leu His Ile
        755                 760                 765             
Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr Pro Pro Leu
    770                 775                 780                 
Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu Ile Thr Gly
785                 790                 795                 800 
Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp Leu His Ala
                805                 810                 815     
Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln His Gly Gln
            820                 825                 830         
Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu Gly Leu Arg
        835                 840                 845             
Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser Gly Asn Lys
    850                 855                 860                 
Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu Phe Gly Thr
865                 870                 875                 880 
Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu Asn Ser Cys
                885                 890                 895     
Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro Glu Gly Cys
            900                 905                 910         
Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn Val Ser Arg
        915                 920                 925             
Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly Glu Pro Arg
    930                 935                 940                 
Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro Glu Cys Leu
945                 950                 955                 960 
Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro Asp Asn Cys
                965                 970                 975     
Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val Lys Thr Cys
            980                 985                 990         
Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp Lys Tyr Ala
        995                 1000                1005            
Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys Thr Tyr Gly
    1010                1015                1020                
Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly Pro Lys Ile
1025                1030                1035               1040 
Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu Leu Leu Val
                1045                1050                1055    
Val Ala Leu Gly Ile Gly Leu Phe Met
            1060                1065


<210> 3
<211> 1068
<212> PRT
<213> Artificial Sequence

<220> 
<223> 4M5.3-CAR AMINO ACID SEQUENCE

<400> 3
Met Leu Leu Leu Val Thr Ser Leu Leu Leu Cys Glu Leu Pro His Pro
 1               5                  10                  15      
Ala Phe Leu Leu Ile Pro Asp Val Val Met Thr Gln Thr Pro Leu Ser
            20                  25                  30          
Leu Pro Val Ser Leu Gly Asp Gln Ala Ser Ile Ser Cys Arg Ser Ser
        35                  40                  45              
Gln Ser Leu Val His Ser Asn Gly Asn Thr Tyr Leu Arg Trp Tyr Leu
    50                  55                  60                  
Gln Lys Pro Gly Gln Ser Pro Lys Val Leu Ile Tyr Lys Val Ser Asn
65                  70                  75                  80  
Arg Val Ser Gly Val Pro Asp Arg Phe Ser Gly Ser Gly Ser Gly Thr
                85                  90                  95      
Asp Phe Thr Leu Lys Ile Asn Arg Val Glu Ala Glu Asp Leu Gly Val
            100                 105                 110         
Tyr Phe Cys Ser Gln Ser Thr His Val Pro Trp Thr Phe Gly Gly Gly
        115                 120                 125             
Thr Lys Leu Glu Ile Lys Ser Ser Ala Asp Asp Ala Lys Lys Asp Ala
    130                 135                 140                 
Ala Lys Lys Asp Asp Ala Lys Lys Asp Asp Ala Lys Lys Asp Gly Gly
145                 150                 155                 160 
Val Lys Leu Asp Glu Thr Gly Gly Gly Leu Val Gln Pro Gly Gly Ala
                165                 170                 175     
Met Lys Leu Ser Cys Val Thr Ser Gly Phe Thr Phe Gly His Tyr Trp
            180                 185                 190         
Met Asn Trp Val Arg Gln Ser Pro Glu Lys Gly Leu Glu Trp Val Ala
        195                 200                 205             
Gln Phe Arg Asn Lys Pro Tyr Asn Tyr Glu Thr Tyr Tyr Ser Asp Ser
    210                 215                 220                 
Val Lys Gly Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Ser Ser Val
225                 230                 235                 240 
Tyr Leu Gln Met Asn Asn Leu Arg Val Glu Asp Thr Gly Ile Tyr Tyr
                245                 250                 255     
Cys Thr Gly Ala Ser Tyr Gly Met Glu Tyr Leu Gly Gln Gly Thr Ser
            260                 265                 270         
Val Thr Val Ser Glu Ser Lys Tyr Gly Pro Pro Cys Pro Pro Cys Pro
        275                 280                 285             
Ala Pro Glu Phe Asp Gly Gly Pro Ser Val Phe Leu Phe Pro Pro Lys
    290                 295                 300                 
Pro Lys Asp Thr Leu Met Ile Ser Arg Thr Pro Glu Val Thr Cys Val
305                 310                 315                 320 
Val Val Asp Val Ser Gln Glu Asp Pro Glu Val Gln Phe Asn Trp Tyr
                325                 330                 335     
Val Asp Gly Val Glu Val His Asn Ala Lys Thr Lys Pro Arg Glu Glu
            340                 345                 350         
Gln Phe Gln Ser Thr Tyr Arg Val Val Ser Val Leu Thr Val Leu His
        355                 360                 365             
Gln Asp Trp Leu Asn Gly Lys Glu Tyr Lys Cys Lys Val Ser Asn Lys
    370                 375                 380                 
Gly Leu Pro Ser Ser Ile Glu Lys Thr Ile Ser Lys Ala Lys Gly Gln
385                 390                 395                 400 
Pro Arg Glu Pro Gln Val Tyr Thr Leu Pro Pro Ser Gln Glu Glu Met
                405                 410                 415     
Thr Lys Asn Gln Val Ser Leu Thr Cys Leu Val Lys Gly Phe Tyr Pro
            420                 425                 430         
Ser Asp Ile Ala Val Glu Trp Glu Ser Asn Gly Gln Pro Glu Asn Asn
        435                 440                 445             
Tyr Lys Thr Thr Pro Pro Val Leu Asp Ser Asp Gly Ser Phe Phe Leu
    450                 455                 460                 
Tyr Ser Arg Leu Thr Val Asp Lys Ser Arg Trp Gln Glu Gly Asn Val
465                 470                 475                 480 
Phe Ser Cys Ser Val Met His Glu Ala Leu His Asn His Tyr Thr Gln
                485                 490                 495     
Lys Ser Leu Ser Leu Ser Leu Gly Lys Met Phe Trp Val Leu Val Val
            500                 505                 510         
Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val Thr Val Ala Phe
        515                 520                 525             
Ile Ile Phe Trp Val Lys Arg Gly Arg Lys Lys Leu Leu Tyr Ile Phe
    530                 535                 540                 
Lys Gln Pro Phe Met Arg Pro Val Gln Thr Thr Gln Glu Glu Asp Gly
545                 550                 555                 560 
Cys Ser Cys Arg Phe Pro Glu Glu Glu Glu Gly Gly Cys Glu Leu Arg
                565                 570                 575     
Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly Gln
            580                 585                 590         
Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr Asp
        595                 600                 605             
Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys Pro
    610                 615                 620                 
Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln Lys Asp
625                 630                 635                 640 
Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu Arg Arg
                645                 650                 655     
Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr Ala Thr
            660                 665                 670         
Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro Arg Leu
        675                 680                 685             
Glu Gly Gly Gly Glu Gly Arg Gly Ser Leu Leu Thr Cys Gly Asp Val
    690                 695                 700                 
Glu Glu Asn Pro Gly Pro Arg Met Leu Leu Leu Val Thr Ser Leu Leu
705                 710                 715                 720 
Leu Cys Glu Leu Pro His Pro Ala Phe Leu Leu Ile Pro Arg Lys Val
                725                 730                 735     
Cys Asn Gly Ile Gly Ile Gly Glu Phe Lys Asp Ser Leu Ser Ile Asn
            740                 745                 750         
Ala Thr Asn Ile Lys His Phe Lys Asn Cys Thr Ser Ile Ser Gly Asp
        755                 760                 765             
Leu His Ile Leu Pro Val Ala Phe Arg Gly Asp Ser Phe Thr His Thr
    770                 775                 780                 
Pro Pro Leu Asp Pro Gln Glu Leu Asp Ile Leu Lys Thr Val Lys Glu
785                 790                 795                 800 
Ile Thr Gly Phe Leu Leu Ile Gln Ala Trp Pro Glu Asn Arg Thr Asp
                805                 810                 815     
Leu His Ala Phe Glu Asn Leu Glu Ile Ile Arg Gly Arg Thr Lys Gln
            820                 825                 830         
His Gly Gln Phe Ser Leu Ala Val Val Ser Leu Asn Ile Thr Ser Leu
        835                 840                 845             
Gly Leu Arg Ser Leu Lys Glu Ile Ser Asp Gly Asp Val Ile Ile Ser
    850                 855                 860                 
Gly Asn Lys Asn Leu Cys Tyr Ala Asn Thr Ile Asn Trp Lys Lys Leu
865                 870                 875                 880 
Phe Gly Thr Ser Gly Gln Lys Thr Lys Ile Ile Ser Asn Arg Gly Glu
                885                 890                 895     
Asn Ser Cys Lys Ala Thr Gly Gln Val Cys His Ala Leu Cys Ser Pro
            900                 905                 910         
Glu Gly Cys Trp Gly Pro Glu Pro Arg Asp Cys Val Ser Cys Arg Asn
        915                 920                 925             
Val Ser Arg Gly Arg Glu Cys Val Asp Lys Cys Asn Leu Leu Glu Gly
    930                 935                 940                 
Glu Pro Arg Glu Phe Val Glu Asn Ser Glu Cys Ile Gln Cys His Pro
945                 950                 955                 960 
Glu Cys Leu Pro Gln Ala Met Asn Ile Thr Cys Thr Gly Arg Gly Pro
                965                 970                 975     
Asp Asn Cys Ile Gln Cys Ala His Tyr Ile Asp Gly Pro His Cys Val
            980                 985                 990         
Lys Thr Cys Pro Ala Gly Val Met Gly Glu Asn Asn Thr Leu Val Trp
        995                 1000                1005            
Lys Tyr Ala Asp Ala Gly His Val Cys His Leu Cys His Pro Asn Cys
    1010                1015                1020                
Thr Tyr Gly Cys Thr Gly Pro Gly Leu Glu Gly Cys Pro Thr Asn Gly
1025                1030                1035               1040 
Pro Lys Ile Pro Ser Ile Ala Thr Gly Met Val Gly Ala Leu Leu Leu
                1045                1050                1055    
Leu Leu Val Val Ala Leu Gly Ile Gly Leu Phe Met
            1060                1065            


<210> 4
<211> 3207
<212> DNA
<213> Artificial Sequence

<220> 
<223> 4M5.3-CAR NUCLEOTIDE ACID SEQUENCE

<400> 4
atgcttctcc tggtgacaag ccttctgctc tgtgagttac cacacccagc attcctcctg 60
atcccagacg ttgtaatgac ccagacccct ctgtctctcc ccgtaagctt gggcgaccag 120
gcgagcatct cttgtcggtc ttcccagtcc ctggtccatt caaacggcaa tacttacttg 180
cggtggtact tgcagaagcc cggtcaatcc ccaaaagtgc tgatatacaa ggttagcaat 240
cgggtcagtg gagtgcccga ccgcttcagc ggaagcggat ccgggactga cttcactctg 300
aagatcaacc gggtagaagc tgaagacctg ggggtgtact tctgctctca gtcaacacac 360
gtgccatgga cctttggagg tggcaccaag ctggaaatca aatcatcagc ggacgatgcc 420
aaaaaagacg cggccaagaa ggacgatgcc aagaaggatg atgctaaaaa ggatggcgga 480
gtcaaattgg acgagacagg cgggggactg gtgcagcccg gcggtgccat gaaactgtct 540
tgtgtgacca gcggctttac cttcgggcat tattggatga actgggtgcg acagtctcca 600
gagaaagggc tcgagtgggt ggcccagttt cgaaataaac cgtacaatta tgagacctac 660
tattcagatt ctgtgaaagg gcgcttcact atttcacgcg acgacagcaa aagttccgtc 720
taccttcaga tgaacaacct tagagtggag gataccggaa tatactactg cacgggtgcc 780
agttatggca tggagtactt ggggcagggg acatctgtga ccgtttctga gagcaagtac 840
ggaccgccct gccccccttg ccctgccccc gagttcgacg gcggacccag cgtgttcctg 900
ttccccccca agcccaagga caccctgatg atcagccgga cccccgaggt gacctgcgtg 960
gtggtggacg tgagccagga agatcccgag gtccagttca attggtacgt ggacggcgtg 1020
gaagtgcaca acgccaagac caagcccaga gaggaacagt tccagagcac ctaccgggtg 1080
gtgtctgtgc tgaccgtgct gcaccaggac tggctgaacg gcaaagaata caagtgcaag 1140
gtgtccaaca agggcctgcc cagcagcatc gaaaagacca tcagcaaggc caagggccag 1200
cctcgcgagc cccaggtgta caccctgcct ccctcccagg aagagatgac caagaaccag 1260
gtgtccctga cctgcctggt gaagggcttc taccccagcg acatcgccgt ggagtgggag 1320
agcaacggcc agcctgagaa caactacaag accacccctc ccgtgctgga cagcgacggc 1380
agcttcttcc tgtacagccg gctgaccgtg gacaagagcc ggtggcagga aggcaacgtc 1440
tttagctgca gcgtgatgca cgaggccctg cacaaccact acacccagaa gagcctgagc 1500
ctgtccctgg gcaagatgtt ctgggtgctg gtggtggtgg gcggggtgct ggcctgctac 1560
agcctgctgg tgacagtggc cttcatcatc ttttgggtga aacggggcag aaagaaactc 1620
ctgtatatat tcaaacaacc atttatgaga ccagtacaaa ctactcaaga ggaagatggc 1680
tgtagctgcc gatttccaga agaagaagaa ggaggatgtg aactgcgggt gaagttcagc 1740
agaagcgccg acgcccctgc ctaccagcag ggccagaatc agctgtacaa cgagctgaac 1800
ctgggcagaa gggaagagta cgacgtcctg gataagcgga gaggccggga ccctgagatg 1860
ggcggcaagc ctcggcggaa gaacccccag gaaggcctgt ataacgaact gcagaaagac 1920
aagatggccg aggcctacag cgagatcggc atgaagggcg agcggaggcg gggcaagggc 1980
cacgacggcc tgtatcaggg cctgtccacc gccaccaagg atacctacga cgccctgcac 2040
atgcaggccc tgcccccaag gctcgagggc ggcggagagg gcagaggaag tcttctaaca 2100
tgcggtgacg tggaggagaa tcccggccct aggatgcttc tcctggtgac aagccttctg 2160
ctctgtgagt taccacaccc agcattcctc ctgatcccac gcaaagtgtg taacggaata 2220
ggtattggtg aatttaaaga ctcactctcc ataaatgcta cgaatattaa acacttcaaa 2280
aactgcacct ccatcagtgg cgatctccac atcctgccgg tggcatttag gggtgactcc 2340
ttcacacata ctcctcctct ggatccacag gaactggata ttctgaaaac cgtaaaggaa 2400
atcacagggt ttttgctgat tcaggcttgg cctgaaaaca ggacggacct ccatgccttt 2460
gagaacctag aaatcatacg cggcaggacc aagcaacatg gtcagttttc tcttgcagtc 2520
gtcagcctga acataacatc cttgggatta cgctccctca aggagataag tgatggagat 2580
gtgataattt caggaaacaa aaatttgtgc tatgcaaata caataaactg gaaaaaactg 2640
tttgggacct ccggtcagaa aaccaaaatt ataagcaaca gaggtgaaaa cagctgcaag 2700
gccacaggcc aggtctgcca tgccttgtgc tcccccgagg gctgctgggg cccggagccc 2760
agggactgcg tctcttgccg gaatgtcagc cgaggcaggg aatgcgtgga caagtgcaac 2820
cttctggagg gtgagccaag ggagtttgtg gagaactctg agtgcataca gtgccaccca 2880
gagtgcctgc ctcaggccat gaacatcacc tgcacaggac ggggaccaga caactgtatc 2940
cagtgtgccc actacattga cggcccccac tgcgtcaaga cctgcccggc aggagtcatg 3000
ggagaaaaca acaccctggt ctggaagtac gcagacgccg gccatgtgtg ccacctgtgc 3060
catccaaact gcacctacgg atgcactggg ccaggtcttg aaggctgtcc aacgaatggg 3120
cctaagatcc cgtccatcgc cactgggatg gtgggggccc tcctcttgct gctggtggtg 3180
gccctgggga tcggcctctt catgtga                                     3207
