                         SEQUENCE LISTING

<110>  OXFORD NANOPORE TECHNOLOGIES LIMITED
 
<120>  POLYNUCLEOTIDE SYNTHESIS METHOD, KIT AND SYSTEM

<130>  N412179WO

<140>  PCT/GB2019/050296
<141>  2019-02-04

<150>  GB 1801768.1
<151>  2018-02-02

<160>  19    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Nucleic acid portion of morpholino oligonucleotide attachment 
       tether

<400>  1
ggaacctctc tgacaa                                                       16


<210>  2
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide synthesis molecule complement (hybridizes to pore)
       (DL1)

<400>  2
gttacccaga tgtgcgatta ctaagtcaga gaggttcc                               38


<210>  3
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  pore attachment (DL1)

<400>  3
gtcagagagg ttcc                                                         14


<210>  4
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide synthesis molecule complementary sequence (DL1)

<400>  4
cagatgtgcg attac                                                        15


<210>  5
<211>  32
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Polynucleotide synthesis molecule (DL2)

<400>  5
gcttgggtgt ttaaccgtaa tcgcacatct gg                                     32


<210>  6
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Feeder molecule (a) attached to transfer nucleotide (A) (DL3)

<400>  6
gtaacttttt tttttctcac agaacaatta ggtaaccagt aacttttttt tttttttttt       60

tttttttt                                                                68


<210>  7
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  encoder sequence for encoding the stored information (DL3)

<400>  7
tttttttttt                                                              10


<210>  8
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  a universal sequence, for hybridisation of a complementary 
       oligonucleotide (DL5)

<400>  8
ctcacagaac aattaggtaa ccagtaac                                          28


<210>  9
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Barcode (DL3)

<400>  9
tttttttttt tttttttttt ttttt                                             25


<210>  10
<211>  68
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Feeder molecule (b) attached to transfer nucleotide (B) (DL4)

<400>  10
gtaacccccc cccccctcac agaacaatta ggtaaccagt aacccccccc cccccccccc       60

cccccccc                                                                68


<210>  11
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  encoder sequence for encoding the stored information (DL4)

<400>  11
cccccccccc                                                              10


<210>  12
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  barcode (DL4)

<400>  12
cccccccccc cccccccccc ccccc                                             25


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Hybridization oligonucleotide (DL5)

<400>  13
gttactggtt acctaattgt tctgtgag                                          28


<210>  14
<211>  16
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Portion of DL2 complementary to DL1 as shown in Figure 21A

<400>  14
gtaatcgcac atctgg                                                       16


<210>  15
<211>  58
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Feeder transfer nucleotide (feeder molecule) as shown in Figure 
       21A


<220>
<221>  misc_feature
<222>  (6)..(30)
<223>  n is a, c, g, or t

<400>  15
gtaacnnnnn nnnnnnnnnn nnnnnnnnnn ctcacagaac aattaggtaa ccagtaac         58


<210>  16
<211>  74
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sequence of feeder transfer nucleotide ligated to DL2 as shown in
       Figures 21B and 21C


<220>
<221>  misc_feature
<222>  (22)..(46)
<223>  n is a, c, g, or t

<400>  16
gtaatcgcac atctgggtaa cnnnnnnnnn nnnnnnnnnn nnnnnnctca cagaacaatt       60

aggtaaccag taac                                                         74


<210>  17
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cleaved portion of hybridization oligonucleotide as shown in Figures 
       21D and 21E

<400>  17
gttacctaat tgttctgtga g                                                 21


<210>  18
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cleaved portion of feeder transfer nucleotide ligated to DL2 as 
       shown in Figures 21D and 21E


<220>
<221>  misc_feature
<222>  (22)..(46)
<223>  n is a, c, g, or t

<400>  18
gtaatcgcac atctgggtaa cnnnnnnnnn nnnnnnnnnn nnnnnnctca cagaacaatt       60

ag                                                                      62


<210>  19
<211>  12
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Cleaved portion of feeder transfer nucleotide ligated to DL2 as 
       shown in Figure 21D

<400>  19
gtaaccagta ac                                                           12


