                               SEQUENCE LISTING

<110> ZYMERGEN INC.
 
<120> GENOME EDITING USING CRISPR IN CORYNEBACTERIUM

<130> ZYMR-038/02WO

<150> US 62/701,979
<151> 2018-07-23

<150> US 62/628,166
<151> 2018-02-08

<160> 13    

<170> PatentIn version 3.5

<210> 1
<211> 1451
<212> DNA
<213> Artificial Sequence

<220>
<223> Synthetic polynucleotide

<400> 1
atctgacttg gttacgatgg actttgaaca cgccgagggt gactaaaccg ctggatttac       60

gcggttttct tctcttcaac ttctttgacg tagcggtgaa ccgtgcccac cgagcaacca      120

atctctgccg cgatagcgcg catggacaga cctttggcgc gcagctcctg gacgcgagca      180

cgcttctcgt tggcacgttt aatgaacact tcacgcggtt cggaagtcca tcgttgagct      240

gttctcaccg acataccggc acgttctgcc agctcgcggg ctgtttttcc gttgcgtgga      300

taacgtgcat aaaccttagc caatgttcct ccaaagagta tgtccagcct cacgacgcac      360

ctcagcgctt cgttgccagc ctttcttccc gcgtgcggat tgcattgcgg tgaatgtggc      420

gtcgtagacg gcggcgccgt ctgtccacat gcgtgacttg gtgatgatcc atttatggat      480

tgacctggca atacagtcaa cttcggccac aggtagtggt tcatcaaaca gctcttggtt      540

aagtgcttgc gcggtggttt ggattgcgcg gcctaggccg tcagcgtctc caaaatgctt      600

tctgacctcc cgatatgccc acgtacgtgc gctttcaaag agtgcgcaat tacgacctag      660

accaactggc gagaaccgcc gcgttttcct ccaggacgca ggcggcataa agccggtttc      720

ttcgagccaa aagcgcagct catcgagcgt atacagctta tcggtgatcc agtgactatc      780

ccatgcagtg tgctcggggt ttttggtgat cagcccggag tatccgctat cgccatcgac      840

agagcgccgt aggccttcgg tgacagccgc ggcataggcc aaaggcttgc gtttggcgta      900

ttcggtgcgg gtaaatggct ccgcgagcgc ccagacagcg tgtgcgtgcc cgtttaaggg      960

gttttcaacc accgcgttag gtctccagtc ctccctgtcc cacaaagagc gcaaaagcgc     1020

gtcctcctgg tcgatgtcaa cgaccaggag gttagagagc gcgtcgggat tggcttcgac     1080

gtagcgctta tccagcgcgt tcttccgtga ggtgcggtaa atgccctcac ggaggtcatc     1140

gcttgccaat ggccacagcg gcagccacag ctgctcaaag cgtccctcag ggcgggtagt     1200

tggtctcatg tagctgactt tctcacacga gcgtgcacgg tcggttttca ttcctcggcg     1260

tgttcataat acgacattta accaagtcag atgtttcccc ggtttccggg ggttcccctg     1320

aagaaccctt ccagtgcgag cgaagcgagc tcctttggcc ggcgcccctc aggtagccct     1380

ctaaggctcc cagggctccg cccctccctg aggttggctc aagcctcctg gtggctccta     1440

cggacgttct a                                                          1451


<210> 2
<211> 1885
<212> DNA
<213> Artificial Sequence

<220>
<223> Synthetic polynucleotide

<400> 2
ggaaccgtta tctgcctatg gtgtgagccc ccctagagag cttcaagagc aatcagcccg       60

acctagaaag gaggccaaga gagagacccc tacgggggga accgttttct gcctacgaga      120

tggcacattt actgggaagc tttacggcgt cctcgtggaa gttcaatgcc cgcagactta      180

agtgctctat tcacggtctg acgtgacacg ctaaattcag acatagcttc attgattgtc      240

ggccacgagc cagtctctcc ctcaacagtc ataaaccaac ctgcaatggt caagcgattt      300

cctttagctt tcctagcttg tcgttgactg gacttagcta gtttttctcg ctgtgctcgg      360

gcgtactcac tgtttgggtc tttccagcgt tctgcggcct ttttaccgcc acgtcttccc      420

atagtggcca gagcttttcg ccctcggctg ctctgcgtct ctgtctgacg agcagggacg      480

actggctggc ctttagcgac gtagccgcgc acacgtcgcg ccatcgtctg gcggtcacgc      540

atcggcggca gatcaggctc acggccgtct gctccgaccg cctgagcgac ggtgtaggca      600

cgctcgtagg cgtcgatgat cttggtgtct tttaggcgct caccagccgc ttttaactgg      660

tatcccacag tcaaagcgtg gcgaaaagcc gtctcatcac gggcggcacg ccctggagca      720

gtccagagga cacggacgcc gtcgatcagc tctccagacg cttcagcggc gctcggcagg      780

cttgcttcaa gcgtggcaag tgcttttgct tccgcagtgg cttttcttgc cgcttcgata      840

cgtgcccgtc cgctagaaaa ctcctgctca tagcgttttt taggtttttc tgtgcctgag      900

atcatgcgag caacctccat aagatcagct aggcgatcca cgcgattgtg ctgggcatgc      960

cagcggtacg cggtgggatc gtcggagacg tgcagtggcc accggctcag cctatgtgaa     1020

aaagcctggt cagcgccgaa aacgcgggtc atttcctcgg tcgttgcagc cagcaggcgc     1080

atattcgggc tgctcatgcc tgctgcggca tacaccggat caatgagcca gatgagctgg     1140

catttcccgc tcagtggatt cacgccgatc caagctggcg ctttttccag gcgtgcccag     1200

cgctccaaaa tcgcgtagac ctcggggttt acgtgctcga ttttcccgcc ggcctggtgg     1260

ctcggcacat caatgtccag gacaagcacg gctgcgtgct gcgcgtgcgt cagagcaaca     1320

tactggcacc gggcaagcga ttttgaacca actcggtata acttcggctg tgtttctccc     1380

gtgtccgggt ctttgatcca agcgctggcg aagtcgcggg tcttgctgcc ctggaaattt     1440

tctctgccca ggtgagcgag gaattcgcgg cggtcttcgc tcgtccagcc acgtgatcgc     1500

agcgcgagct cgggatgggt gtcgaacaga tcagcggaaa atttccaggc cggtgtgtca     1560

atgtctcgtg aatccgctag agtcattttt gagcgctttc tcccaggttt ggactggggg     1620

ttagccgacg ccctgtgagt taccgctcac ggggcgttca acatttttca ggtattcgtg     1680

cagcttatcg cttcttgccg cctgtgcgct ttttcgacgc gcgacgctgc tgccgattcg     1740

gtgcaggtgg tggcggcgct gacacgtcct gggcggccac ggccacacga aacgcggcat     1800

ttacgatgtt tgtcatgcct gcgggcaccg cgccacgatc gcggataatt ctcgctgccg     1860

cttccagctc tgtgacgacc atgct                                           1885


<210> 3
<211> 4107
<212> DNA
<213> Artificial Sequence

<220>
<223> Synthetic polynucleotide

<400> 3
atggacaaga agtacagcat cggcctggac atcggcacca acagcgtggg ctgggcggtc       60

atcaccgacg agtacaaggt cccctccaag aagttcaagg tcctgggcaa caccgaccgg      120

cactcgatca agaagaacct gatcggcgcc ctgctcttcg acagcggcga aaccgccgag      180

gcgacccgcc tgaagcggac cgcccgtcgc cgctacaccc ggcgcaagaa ccgcatctgc      240

tacctccagg agatcttctc caacgagatg gccaaggtcg acgactcgtt cttccaccgg      300

ctcgaggaga gcttcctggt ggaggaggac aagaagcacg agcgccaccc gatcttcggc      360

aacatcgtcg acgaggtggc ctaccacgag aagtacccca ccatctacca cctccgcaag      420

aagctggtcg actcgaccga caaggcggac ctgcggctca tctacctggc cctcgcgcac      480

atgatcaagt tccgcggcca cttcctcatc gagggcgacc tgaacccgga caactccgac      540

gtcgacaagc ttttcatcca gctggtgcag acctacaacc agctgttcga ggagaacccc      600

atcaacgcca gcggcgtcga cgccaaggcg atcctctccg cgcgcctgag caagtcccgg      660

cgcctggaga acctcatcgc ccagctgccg ggcgagaaga agaacggcct cttcggcaac      720

ctgatcgcgc tgtcgctcgg cctgaccccc aacttcaaga gcaacttcga cctggccgag      780

gacgcgaagc tccagctgtc caaggacacc tacgacgacg acctggacaa cctgctcgcc      840

cagatcggcg accagtacgc ggacctcttc ctggccgcga agaacctctc ggacgccatc      900

ctgctcagcg acatcctgcg ggtcaacacc gagatcacca aggccccgct gtcggcgagc      960

atgatcaagc ggtacgacga gcaccaccag gacctgaccc tgctcaaggc cctcgtgcgc     1020

cagcagctgc ccgagaagta caaggagatc ttcttcgacc agtccaagaa cggctacgcc     1080

ggctacatcg acggcggcgc gtcgcaggag gagttctaca agttcatcaa gccgatcctg     1140

gagaagatgg acggcaccga ggagctgctc gtcaagctga accgcgagga cctgctccgc     1200

aagcagcgga ccttcgacaa cggctccatc ccgcaccaga tccacctggg cgagctccac     1260

gccatcctcc ggcgccagga ggacttctac cccttcctga aggacaaccg cgagaagatc     1320

gagaagatcc tgaccttccg gatcccgtac tacgtcggcc ccctggcccg cggcaactcc     1380

cggttcgcgt ggatgacccg gaagtcggag gaaaccatca ccccgtggaa cttcgaggag     1440

gtcgtggaca agggcgcctc cgcgcagtcg ttcatcgagc gcatgaccaa cttcgacaag     1500

aacctcccga acgagaaggt cctgcccaag cacagcctgc tctacgagta cttcaccgtg     1560

tacaacgagc tgaccaaggt caagtacgtg accgagggca tgcggaagcc ggccttcctg     1620

tccggcgagc agaagaaggc gatcgtcgac ctgctcttca agaccaaccg caaggtcacc     1680

gtgaagcagc tgaaggagga ctacttcaag aagatcgagt gcttcgactc cgtcgagatc     1740

tcgggcgtgg aggaccgctt caacgcctcc ctgggcacct accacgacct gctcaagatc     1800

atcaaggaca aggacttcct cgacaacgag gagaacgagg acatcctgga ggacatcgtc     1860

ctcaccctga ccctcttcga ggaccgcgag atgatcgagg agcggctcaa gacctacgcc     1920

cacctgttcg acgacaaggt gatgaagcag ctgaagcggc gccggtacac cggctggggc     1980

cgcctctccc ggaagctgat caacggcatc cgggacaagc agagcggcaa gaccatcctg     2040

gacttcctca agtccgacgg cttcgccaac cgcaacttca tgcagctcat ccacgacgac     2100

tcgctgacct tcaaggagga catccagaag gcccaggtgt ccggccaggg cgacagcctc     2160

cacgagcaca tcgccaacct ggcgggctcc ccggcgatca agaagggcat cctccagacc     2220

gtcaaggtcg tggacgagct ggtcaaggtg atgggccgcc acaagcccga gaacatcgtg     2280

atcgagatgg cccgggagaa ccagaccacc cagaagggcc agaagaactc ccgcgagcgg     2340

atgaagcgca tcgaggaggg catcaaggag ctcggctcgc agatcctgaa ggagcacccg     2400

gtcgagaaca cccagctcca gaacgagaag ctgtacctct actacctgca gaacggccgc     2460

gacatgtacg tggaccagga gctcgacatc aaccggctga gcgactacga cgtcgaccac     2520

atcgtgccgc agtccttcct gaaggacgac tcgatcgaca acaaggtcct gacccgctcc     2580

gacaagaacc ggggcaagtc cgacaacgtg ccctcggagg aggtcgtgaa gaagatgaag     2640

aactactggc gccagctgct caacgccaag ctcatcaccc agcgcaagtt cgacaacctg     2700

accaaggccg agcggggcgg cctgtcggag ctcgacaagg cgggcttcat caagcgccag     2760

ctcgtcgaaa cccggcagat caccaagcac gtggcccaga tcctggacag ccggatgaac     2820

accaagtacg acgagaacga caagctgatc cgcgaggtca aggtgatcac cctcaagagc     2880

aagctggtgt ccgacttccg caaggacttc cagttctaca aggtccggga gatcaacaac     2940

taccaccacg cccacgacgc gtacctgaac gccgtcgtgg gcaccgcgct gatcaagaag     3000

tacccgaagc tggagtccga gttcgtctac ggcgactaca aggtctacga cgtgcgcaag     3060

atgatcgcca agtcggagca ggagatcggc aaggccaccg cgaagtactt cttctacagc     3120

aacatcatga acttcttcaa gaccgagatc accctggcca acggcgagat ccgcaagcgg     3180

cccctgatcg aaaccaacgg cgaaaccggc gagatcgtct gggacaaggg ccgcgacttc     3240

gccaccgtcc ggaaggtgct gtccatgccg caggtcaaca tcgtcaagaa aaccgaggtg     3300

cagaccggcg gcttcagcaa ggagtccatc ctccccaagc gcaactcgga caagctgatc     3360

gcccggaaga aggactggga cccgaagaag tacggcggct tcgacagccc caccgtcgcc     3420

tactccgtgc tggtcgtggc gaaggtcgag aagggcaaga gcaagaagct gaagtccgtg     3480

aaggagctgc tcggcatcac catcatggag cgctcctcgt tcgagaagaa cccgatcgac     3540

ttcctggagg ccaagggcta caaggaggtc aagaaggacc tcatcatcaa gctgcccaag     3600

tactcgctgt tcgagctcga gaacggccgc aagcggatgc tcgccagcgc gggcgagctg     3660

cagaagggca acgagctggc cctcccgtcc aagtacgtca acttcctgta cctcgcgtcc     3720

cactacgaga agctgaaggg ctcgcccgag gacaacgagc agaagcagct tttcgtggag     3780

cagcacaagc actacctgga cgagatcatc gagcagatct cggagttcag caagcgggtc     3840

atcctggccg acgcgaacct cgacaaggtg ctgtccgcct acaacaagca ccgcgacaag     3900

ccgatccggg agcaggcgga gaacatcatc cacctgttca ccctcaccaa cctgggcgcc     3960

cccgccgcgt tcaagtactt cgacaccacc atcgaccgca agcggtacac cagcaccaag     4020

gaggtcctcg acgcgaccct gatccaccag tccatcaccg gcctgtacga aacccgcatc     4080

gacctctccc agctcggcgg cgactga                                         4107


<210> 4
<211> 59
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 4
cgtcaagatc acccaaaact ggtggctgtt ctcttttaag cgggatagca tgggttctt        59


<210> 5
<211> 97
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 5
tgccgtttct cgcgttgtgt gtggtactac gtggggacct aagcgtgtaa gatggaaacg       60

tctgtatcgg ataagtagcg aggagtgttc gttaaaa                                97


<210> 6
<211> 92
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 6
taactacatt gagcgaaatg ccaaccacat gtcccatgct tttactaatg tggggtctta       60

gaagaaagcg accaatttaa ggagagttga at                                     92


<210> 7
<211> 49
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 7
agtgaaccca tacttttata tatgggtatc ggcggtctat gcttgtggg                   49


<210> 8
<211> 74
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 8
tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcacatcagc       60

aggacgcact gacc                                                         74


<210> 9
<211> 80
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 9
tcgtcaagat cacccaaaac tggtggctgt tctcttttaa gcgggatagc atgggttctt       60

atccctatca gtgatagaga                                                   80


<210> 10
<211> 62
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 10
ttgacaatta atcatcggct cgtataatgt gtggaattgt gagcggataa caatttcaca       60

ca                                                                      62


<210> 11
<211> 101
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 11
ctcgagggta aatgtgagca ctcacaattc attttgcaaa agttgttgac tttatctaca       60

aggtgtggca taatgtgtgt aattgtgagc ggataacaat t                          101


<210> 12
<211> 354
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 12
acttttcata ctcccgccat tcagagaaga aaccaattgt ccatattgca tcagacattg       60

ccgtcactgc gtcttttact ggctcttctc gctaaccaaa ccggtaaccc cgcttattaa      120

aagcattctg taacaaagcg ggaccaaagc catgacaaaa acgcgtaaca aaagtgtcta      180

taatcacggc agaaaagtcc acattgatta tttgcacggc gtcacacttt gctatgccat      240

agcattttta tccataagat tagcggatcc tacctgacgc tttttatcgc aactctctac      300

tgtttctcca tacccgtttt tttgggaatt cgagctctaa ggaggttata aaaa            354


<210> 13
<211> 226
<212> DNA
<213> Unknown

<220>
<223> promoter sequence

<400> 13
gagctgttga caattaatca tccggctcgt ataatgtgtg gaattgtgag cggataacaa       60

tttcacacag gaaacagcgc cgctgagaaa aagcgaagcg gcactgctct ttaacaattt      120

atcagacaat ctgtgtgggc actcgaccgg aattatcgat taactttatt attaaaaatt      180

aaagaggtat atattaatgt atcgattaaa taaggaggaa taaacc                     226


