                         SEQUENCE LISTING

<110>  BIOLOGICAL E LIMITED
 
<120>  EXPRESSION OF PNEUMOCOCCAL SURFACE PROTEIN A

<130>  pspA1

<160>  15    

<170>  PatentIn version 3.5

<210>  1
<211>  795
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Kanamycin Resistance Gene

<400>  1
atgattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc       60

ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt ccggctgtca      120

gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactg      180

caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg      240

ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag      300

gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg      360

cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc      420

atcgagcgag cacgtactcg ggatggagcc gggtcttgtc gatcagatga tctggacgaa      480

gagcatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac      540

ggcgaggatc tcgtcgtgac ccatggcgat gcctgcttgc cgaatatcat ggtggaaaat      600

ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac      660

atagcgttgg ctacccgtga tattgctgaa gagcttggcg gcgaatgggc tgaccgcttc      720

ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt      780

gacgagttct tctga                                                       795


<210>  2
<211>  30
<212>  DNA
<213>  ARTIFICIAL

<220>
<223>  Hybrid Promoter

<400>  2
ttgacaatta atcatcggct cgtataatgt                                        30


<210>  3
<211>  310
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  3

Glu Asp Ala Pro Val Ala Asn Gln Ser Lys Ala Glu Lys Asp Tyr Asp 
1               5                   10                  15      


Ala Ala Lys Arg Asp Ala Glu Asn Ala Lys Lys Ala Leu Glu Asp Ala 
            20                  25                  30          


Lys Arg Ala Gln Lys Lys Tyr Lys Asp Asp Gln Lys Ile Thr Glu Glu 
        35                  40                  45              


Lys Ala Glu Glu Glu Lys Lys Ala Ser Gln Glu Gln Gln Lys Ala Asn 
    50                  55                  60                  


Leu Asp Tyr Gln Gln Lys Leu Arg Lys Tyr Ile Asn Glu Lys Asp Ser 
65                  70                  75                  80  


Lys Lys Arg Ser Met Leu Gln Lys Glu Met Glu Glu Ala Glu Arg Lys 
                85                  90                  95      


Asp Lys Glu Lys Gln Ala Glu Phe Lys Lys Ile Arg Glu Lys Val Ile 
            100                 105                 110         


Pro Ser Ala Glu Glu Leu Thr Glu Thr Arg Arg Lys Ala Glu Glu Ala 
        115                 120                 125             


Glu Ala Lys Glu Pro Glu Leu Thr Lys Lys Val Lys Glu Ala Glu Glu 
    130                 135                 140                 


Lys Val Thr Glu Ala Lys Gln Lys Leu Asp Ala Glu Arg Ala Lys Glu 
145                 150                 155                 160 


Val Ala Leu Gln Ala Lys Ile Ala Glu Leu Glu Asn Glu Val His Arg 
                165                 170                 175     


Leu Glu Thr Lys Leu Lys Glu Ile Asp Glu Ser Asp Ser Glu Asp Tyr 
            180                 185                 190         


Val Lys Glu Gly Leu Arg Ala Pro Leu Gln Ser Glu Leu Asp Ala Lys 
        195                 200                 205             


Gln Ala Lys Leu Ser Lys Leu Glu Glu Leu Ser Asp Lys Ile Asp Glu 
    210                 215                 220                 


Leu Asp Ala Glu Ile Ala Lys Leu Glu Lys Asp Val Glu Asp Phe Lys 
225                 230                 235                 240 


Asn Ser Asp Gly Glu Tyr Ser Ala Leu Tyr Leu Glu Ala Ala Glu Lys 
                245                 250                 255     


Asp Leu Ala Ala Lys Lys Ala Glu Leu Glu Lys Thr Glu Ala Asp Leu 
            260                 265                 270         


Lys Lys Ala Val Asp Glu Pro Glu Lys Pro Ala Pro Ala Pro Glu Thr 
        275                 280                 285             


Pro Ala Pro Glu Ala Pro Ala Glu Gln Pro Lys Pro Ala Pro Ala Pro 
    290                 295                 300                 


Gln Pro Ala Pro Ala Pro 
305                 310 


<210>  4
<211>  369
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  4

Glu Asp Ala Pro Val Ala Asn Gln Ser Lys Ala Glu Lys Asp Tyr Asp 
1               5                   10                  15      


Ala Ala Lys Arg Asp Ala Glu Asn Ala Lys Lys Ala Leu Glu Asp Ala 
            20                  25                  30          


Lys Arg Ala Gln Lys Lys Tyr Lys Asp Asp Gln Lys Ile Thr Glu Glu 
        35                  40                  45              


Lys Ala Glu Glu Glu Lys Lys Ala Ser Gln Glu Gln Gln Lys Ala Asn 
    50                  55                  60                  


Leu Asp Tyr Gln Gln Lys Leu Arg Lys Tyr Ile Asn Glu Lys Asp Ser 
65                  70                  75                  80  


Lys Lys Arg Ser Met Leu Gln Lys Glu Met Glu Glu Ala Glu Arg Lys 
                85                  90                  95      


Asp Lys Glu Lys Gln Ala Glu Phe Lys Lys Ile Arg Glu Lys Val Ile 
            100                 105                 110         


Pro Ser Ala Glu Glu Leu Thr Glu Thr Arg Arg Lys Ala Glu Glu Ala 
        115                 120                 125             


Glu Ala Lys Glu Pro Glu Leu Thr Lys Lys Val Lys Glu Ala Glu Glu 
    130                 135                 140                 


Lys Val Thr Glu Ala Lys Gln Lys Leu Asp Ala Glu Arg Ala Lys Glu 
145                 150                 155                 160 


Val Ala Leu Gln Ala Lys Ile Ala Glu Leu Glu Asn Glu Val His Arg 
                165                 170                 175     


Leu Glu Thr Lys Leu Lys Glu Ile Asp Glu Ser Asp Ser Glu Asp Tyr 
            180                 185                 190         


Val Lys Glu Gly Leu Arg Ala Pro Leu Gln Ser Glu Leu Asp Ala Lys 
        195                 200                 205             


Gln Ala Lys Leu Ser Lys Leu Glu Glu Leu Ser Asp Lys Ile Asp Glu 
    210                 215                 220                 


Leu Asp Ala Glu Ile Ala Lys Leu Glu Lys Asp Val Glu Asp Phe Lys 
225                 230                 235                 240 


Asn Ser Asp Gly Glu Tyr Ser Ala Leu Tyr Leu Glu Ala Ala Glu Lys 
                245                 250                 255     


Asp Leu Ala Ala Lys Lys Ala Glu Leu Glu Lys Thr Glu Ala Asp Leu 
            260                 265                 270         


Lys Lys Ala Val Asp Glu Pro Glu Lys Pro Ala Pro Ala Pro Glu Thr 
        275                 280                 285             


Pro Ala Pro Glu Ala Pro Ala Glu Gln Pro Lys Pro Ala Pro Ala Pro 
    290                 295                 300                 


Gln Pro Ala Pro Ala Pro Lys Pro Glu Lys Pro Ala Glu Gln Pro Lys 
305                 310                 315                 320 


Pro Glu Lys Thr Asp Asp Gln Gln Ala Glu Glu Asp Tyr Ala Arg Arg 
                325                 330                 335     


Ser Glu Glu Glu Tyr Asn Arg Leu Thr Gln Gln Gln Pro Pro Lys Ala 
            340                 345                 350         


Glu Lys Pro Ala Pro Ala Pro Lys Pro Glu Gln Pro Ala Pro Ala Pro 
        355                 360                 365             


Lys 
    


<210>  5
<211>  933
<212>  DNA
<213>  Streptococcus pneumoniae

<400>  5
gaagacgctc ctgtagctaa ccagtctaaa gctgagaaag actatgatgc agcgaagaga       60

gatgctgaga atgcgaaaaa agctttagag gacgcaaaac gtgcgcagaa aaaatataag      120

gatgatcaga agataactga ggagaaagcg gaagaagaaa aaaaagcttc tcaagagcaa      180

caaaaagcaa atctggacta tcaacaaaag ttgaggaaat atattaacga aaaagactcc      240

aaaaaaagat ctatgcttca gaaagaaatg gaggaagctg agagaaaaga taaggaaaaa      300

caagcagaat ttaagaagat tagagaaaag gtgattccta gcgcggaaga gttaacagag      360

actagacgaa aagcagaaga ggctgaagca aaagaaccag agcttactaa aaaagtaaaa      420

gaagctgagg aaaaagttac tgaagccaaa caaaaattgg atgctgaacg tgctaaagaa      480

gttgctcttc aagccaaaat cgctgagttg gaaaatgaag ttcatagact agaaacaaaa      540

ctcaaagaga ttgatgaatc tgactcagaa gattatgtta aagaaggtct ccgtgctcct      600

cttcaatctg aattggatgc taagcaagct aaactatcaa aacttgaaga gttgagtgat      660

aagattgatg agttagacgc tgaaattgca aaacttgaaa aagatgtaga agatttcaaa      720

aactcagacg gtgaatattc tgcattatat cttgaagctg cagaaaaaga tttagctgct      780

aaaaaagctg aattagaaaa aactgaagct gacctcaaga aagcagttga tgagccagaa      840

aaaccagctc cagctccaga aactccagcc ccagaagcac cagctgaaca accaaaacca      900

gcgccggctc ctcaaccagc tcctgcacca taa                                   933


<210>  6
<211>  1110
<212>  DNA
<213>  Streptococcus pneumoniae

<400>  6
gaagacgctc ctgtagctaa ccagtctaaa gctgagaaag actatgatgc agcgaagaga       60

gatgctgaga atgcgaaaaa agctttagag gacgcaaaac gtgcgcagaa aaaatataag      120

gatgatcaga agataactga ggagaaagcg gaagaagaaa aaaaagcttc tcaagagcaa      180

caaaaagcaa atctggacta tcaacaaaag ttgaggaaat atattaacga aaaagactcc      240

aaaaaaagat ctatgcttca gaaagaaatg gaggaagctg agagaaaaga taaggaaaaa      300

caagcagaat ttaagaagat tagagaaaag gtgattccta gcgcggaaga gttaacagag      360

actagacgaa aagcagaaga ggctgaagca aaagaaccag agcttactaa aaaagtaaaa      420

gaagctgagg aaaaagttac tgaagccaaa caaaaattgg atgctgaacg tgctaaagaa      480

gttgctcttc aagccaaaat cgctgagttg gaaaatgaag ttcatagact agaaacaaaa      540

ctcaaagaga ttgatgaatc tgactcagaa gattatgtta aagaaggtct ccgtgctcct      600

cttcaatctg aattggatgc taagcaagct aaactatcaa aacttgaaga gttgagtgat      660

aagattgatg agttagacgc tgaaattgca aaacttgaaa aagatgtaga agatttcaaa      720

aactcagacg gtgaatattc tgcattatat cttgaagctg cagaaaaaga tttagctgct      780

aaaaaagctg aattagaaaa aactgaagct gacctcaaga aagcagttga tgagccagaa      840

aaaccagctc cagctccaga aactccagcc ccagaagcac cagctgaaca accaaaacca      900

gcgccggctc ctcaaccagc tcccgcacca aaaccagaga agccagctga acaaccaaaa      960

ccagaaaaaa cagatgatca acaagctgaa gaagactatg ctcgtagatc agaagaagaa     1020

tataaccgct tgactcaaca gcaaccgcca aaagcagaaa aaccagctcc cgcaccaaaa     1080

ccagagcaac cagctcctgc accaaaataa                                      1110


<210>  7
<211>  20
<212>  DNA
<213>  Corynebacterium glutamicum

<400>  7
tagaaaggtg tgtttcaccc                                                   20


<210>  8
<211>  93
<212>  DNA
<213>  Streptococcus pneumoniae

<400>  8
atgaataaga aaaaaatgat tttaacaagt ctagccagcg tcgctatctt aggggctggt       60

tttgttgcgt cttcgcctac tgttgtaaga gca                                    93


<210>  9
<211>  31
<212>  PRT
<213>  Streptococcus pneumoniae

<400>  9

Met Asn Lys Lys Lys Met Ile Leu Thr Ser Leu Ala Ser Val Ala Ile 
1               5                   10                  15      


Leu Gly Ala Gly Phe Val Ala Ser Ser Pro Thr Val Val Arg Ala 
            20                  25                  30      


<210>  10
<211>  38
<212>  DNA
<213>  Streptococcus pneumoniae

<400>  10
aaattaaagc atgttaagaa catttgacat tttaattt                               38


<210>  11
<211>  19
<212>  DNA
<213>  Artificial

<220>
<223>   PT7 Promoter

<400>  11
taatacgact cactatagg                                                    19


<210>  12
<211>  1854
<212>  DNA
<213>  Corynebacterium diphtheriae

<400>  12
gaattcggtg aggttatggc ggagggttgc gaggtctagg agaacagagg aagtcatgct       60

ttgaagcata taagctgccc tgcccctcaa ggttttcttc aagtgaggtt ttatctaact      120

gcctaacggc aggggaaccg tatattgctt acggtatgag accccttaaa cgtccggata      180

gtcaccgctc ttctttagct ccgcgacatg cctagcaacc gtggcgcgag agactcctac      240

ctctgcccct atttcagccc acgtgggaac tgtccctgtc tggaaatact gatcgttcac      300

catttggcta atacgggact tcgtagatcg tccttgagcc tttttcttac ggtgcgtctt      360

ttcaagcttc gacctttgtg cttgcgcata tttgccctcg gggtctgttt tccagcgttg      420

tgcggctttt tgtccgcctc tgcgtcccat cgtggccaag gctttccgct cgctgctggt      480

ggctttacct ggtgcgttag agccgctgta ggtctcgctc ttggattggg cgacataccc      540

gcgcacgcgc cttgccatgg tttggcggtc gcgcatgggt ggcatctcgt tgtcgcggcc      600

tgcaccgccg tgggtgtgtg cgacgttgta ggcgtgctca taggcgtcga tgattgctgc      660

gtctgtcagg cgttggcctt gctggcgcaa gcggtggcca gtcttaagcg catgtctaaa      720

ggctgtttcg tcgcgtgctg cggttccttg gacaatccag agcacacgca caccgtcgat      780

aagttccggg tcatactggt cgagaccacc ggcgatttcc gcgtctacgt cctgggcgag      840

tgctttgaat gcttgggctt cttcacggcg ggtcttgacc gcgttgataa gttcgcggcc      900

agagctgaat tgctggcgtg gggtggggtt gaactggtcg tgtcctgcca tatcccttac      960

ctgctttatc aagtctccaa ggcgcatcac ccggttgtgc tgcctatacc aacgataagc     1020

ggtaggggct ttgcctgtgt agaacgggtt gcggctaaag cggtgggaaa agtgcgggtc     1080

atggtctaaa agctcaccca gcacacgcgt ggttgctgca agaagcttca tctgcgcaga     1140

tttaccgtta cggtcagcgt agacagggtc aataagccat atgaactggg ctttgccgtt     1200

agttgggtta atacccaccc aggctggccc gacgctatga gtaatcagtg agcgcaccac     1260

gtcgcggacg tacgggttta agtctgcggg gtcaccgcct gcggtaccta cttggtcaac     1320

gtctacgacc aggacggcgg cgtactgctt ggtggtgagc atggcgtact cgcaccgtcc     1380

taaagcatca gtctcgaagc gatacatacg cggcgagttc gtgccgtcag cgttgcgtcg     1440

ataggccttt ttaaagtctc gtgtgactga accgtggagt acatcgcggc ctagatgatc     1500

gcgtaaaagg tcgcggtcac tggcagatgc tggggtgttg tccagtccac cacggtcgcg     1560

ctcgacgcgg gtaggtgttt tagtgtgcgc attctgcgca tgagtctgta aactcatgac     1620

cgtgatttct cccaggtgtg tgctgggtga taagcgaaag tcatcgggtt gccgcccggt     1680

ggctttcttc gtttttcatt gtctttccct gactctaaat gacaccggtg ttatttacta     1740

gccatgacac gcgaaaaata tgccttttac ctgcggttac gtatggctag acatatggca     1800

agctatacgt aaccgcgttt cagctgcaca gggctgtctg cgcagattta ccat           1854


<210>  13
<211>  627
<212>  DNA
<213>  Artificial

<220>
<223>  rrnB terminator

<400>  13
cggcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta       60

atcgccttgc agcacatccc cctttcgcca gctggctaat agcgaagagg cccgcaccga      120

tcgcccttcc caacagttgc gcagcctgaa tggcgatggc tgttttggcg gatgagagaa      180

gattttcagc ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt      240

gcctggcggc agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg      300

ccgtagcgcc gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc      360

aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg      420

tgaacgctct cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac      480

ggcccggagg gtggcgggca ggacgcccgc cataaactgc caggcatcaa attaagcaga      540

aggccatcct gacggatggc ctttttgcgt ttctacaaac tcttttgttt atttttctaa      600

atacattcaa atatgtatcc gctcatg                                          627


<210>  14
<211>  3784
<212>  DNA
<213>  Artificial

<220>
<223>  pBE31C

<400>  14
actagtctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg       60

agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg      120

gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac      180

catgattacg aattcccggg gatccgtcga cctgcagcca agcttggcac tggccgtcgt      240

tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca      300

tccccctttc gccagctggc taatagcgaa gaggcccgca ccgatcgccc ttcccaacag      360

ttgcgcagcc tgaatggcga tggctgtttt ggcggatgag agaagatttt cagcctgata      420

cagattaaat cagaacgcag aagcggtctg ataaaacaga atttgcctgg cggcagtagc      480

gcggtggtcc cacctgaccc catgccgaac tcagaagtga aacgccgtag cgccgatggt      540

agtgtggggt ctccccatgc gagagtaggg aactgccagg catcaaataa aacgaaaggc      600

tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctcctgag      660

taggacaaat ccgccgggag cggatttgaa cgttgcgaag caacggcccg gagggtggcg      720

ggcaggacgc ccgccataaa ctgccaggca tcaaattaag cagaaggcca tcctgacgga      780

tggccttttt gcgtttctac aaactctttt gtttattttt ctaaatacat tcaaatatgt      840

atccgctcat gtttaaaact agtccgaggt cccgggggtt gggcgtcgct tggtcggtca      900

tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga aggcgataga aggcgatgcg      960

ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag cggtcagccc attcgccgcc     1020

aagctcttca gcaatatcac gggtagccaa cgctatgtcc tgatagcggt ccgccacacc     1080

cagccggcca cagtcgatga atccagaaaa gcggccattt tccaccatga tattcggcaa     1140

gcaggcatcg ccatgggtca cgacgagatc ctcgccgtcg ggcatgcgcg ccttgagcct     1200

ggcgaacagt tcggctggcg cgagcccctg atgctcttcg tccagatcat ctgatcgaca     1260

agacccggct ccatcccgag tacgtgctcg ctcgatgcga tgtttcgctt ggtggtcgaa     1320

tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt gcatcagcca tgatggatac     1380

tttctcggca ggagcaaggt gagatgacag gagatcctgc cccggcactt cgcccaatag     1440

cagccagtcc cttcccgctt cagtgacaac gtcgagcaca gctgcgcaag gaacgcccgt     1500

cgtggccagc cacgatagcc gcgctgcctc gtcctgcagt tcattcaggg caccggacag     1560

gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac agccggaaca cggcggcatc     1620

agagcagccg attgtctgtt gtgcccagtc atagccgaat agcctctcca cccaagcggc     1680

cggagaacct gcgtgcaatc catcttgttc aatcatgcga aacgatcctc atcctgtctc     1740

ttgatcagat cttgatcccc tgcgccatca gatccttggc ggcaagaaag ccatccagtt     1800

tactttgcag ggcttcccaa ccttaccaga gggcgcccca gctggcaatt ccggttcgct     1860

tgctgtccat aaaaccgccc agtctagcta tcgccatccc gggaccttct agtagatctg     1920

aattcggtga ggttatggcg gagggttgcg aggtctagga gaacagagga agtcatgctt     1980

tgaagcatat aagctgccct gcccctcaag gttttcttca agtgaggttt tatctaactg     2040

cctaacggca ggggaaccgt atattgctta cggtatgaga ccccttaaac gtccggatag     2100

tcaccgctct tctttagctc cgcgacatgc ctagcaaccg tggcgcgaga gactcctacc     2160

tctgccccta tttcagccca cgtgggaact gtccctgtct ggaaatactg atcgttcacc     2220

atttggctaa tacgggactt cgtagatcgt ccttgagcct ttttcttacg gtgcgtcttt     2280

tcaagcttcg acctttgtgc ttgcgcatat ttgccctcgg ggtctgtttt ccagcgttgt     2340

gcggcttttt gtccgcctct gcgtcccatc gtggccaagg ctttccgctc gctgctggtg     2400

gctttacctg gtgcgttaga gccgctgtag gtctcgctct tggattgggc gacatacccg     2460

cgcacgcgcc ttgccatggt ttggcggtcg cgcatgggtg gcatctcgtt gtcgcggcct     2520

gcaccgccgt gggtgtgtgc gacgttgtag gcgtgctcat aggcgtcgat gattgctgcg     2580

tctgtcaggc gttggccttg ctggcgcaag cggtggccag tcttaagcgc atgtctaaag     2640

gctgtttcgt cgcgtgctgc ggttccttgg acaatccaga gcacacgcac accgtcgata     2700

agttccgggt catactggtc gagaccaccg gcgatttccg cgtctacgtc ctgggcgagt     2760

gctttgaatg cttgggcttc ttcacggcgg gtcttgaccg cgttgataag ttcgcggcca     2820

gagctgaatt gctggcgtgg ggtggggttg aactggtcgt gtcctgccat atcccttacc     2880

tgctttatca agtctccaag gcgcatcacc cggttgtgct gcctatacca acgataagcg     2940

gtaggggctt tgcctgtgta gaacgggttg cggctaaagc ggtgggaaaa gtgcgggtca     3000

tggtctaaaa gctcacccag cacacgcgtg gttgctgcaa gaagcttcat ctgcgcagat     3060

ttaccgttac ggtcagcgta gacagggtca ataagccata tgaactgggc tttgccgtta     3120

gttgggttaa tacccaccca ggctggcccg acgctatgag taatcagtga gcgcaccacg     3180

tcgcggacgt acgggtttaa gtctgcgggg tcaccgcctg cggtacctac ttggtcaacg     3240

tctacgacca ggacggcggc gtactgcttg gtggtgagca tggcgtactc gcaccgtcct     3300

aaagcatcag tctcgaagcg atacatacgc ggcgagttcg tgccgtcagc gttgcgtcga     3360

taggcctttt taaagtctcg tgtgactgaa ccgtggagta catcgcggcc tagatgatcg     3420

cgtaaaaggt cgcggtcact ggcagatgct ggggtgttgt ccagtccacc acggtcgcgc     3480

tcgacgcggg taggtgtttt agtgtgcgca ttctgcgcat gagtctgtaa actcatgacc     3540

gtgatttctc ccaggtgtgt gctgggtgat aagcgaaagt catcgggttg ccgcccggtg     3600

gctttcttcg tttttcattg tctttccctg actctaaatg acaccggtgt tatttactag     3660

ccatgacacg cgaaaaatat gccttttacc tgcggttacg tatggctaga catatggcaa     3720

gctatacgta accgcgtttc agctgcacag ggctgtctgc gcagatttac catagatcta     3780

ctag                                                                  3784


<210>  15
<211>  5501
<212>  DNA
<213>  Artificial

<220>
<223>  pBE117

<400>  15
actagtctga aatgagctgt tgacaattaa tcatcggctc gtataatgtg tggaattgtg       60

agcggataac aatttcacac aggaaactag gcaccccagg ctttacactt tatgcttccg      120

gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac      180

catgattacg aattcccggg gatcctagaa aggtgtgttt cacccatgaa taagaaaaaa      240

atgattttaa caagtctagc cagcgtcgct atcttagggg ctggttttgt tgcgtcttcg      300

cctactgttg taagagcaga agacgctcct gtagctaacc agtctaaagc tgagaaagac      360

tatgatgcag cgaagagaga tgctgagaat gcgaaaaaag ctttagagga cgcaaaacgt      420

gcgcagaaaa aatataagga tgatcagaag ataactgagg agaaagcgga agaagaaaaa      480

aaagcttctc aagagcaaca aaaagcaaat ctggactatc aacaaaagtt gaggaaatat      540

attaacgaaa aagactccaa aaaaagatct atgcttcaga aagaaatgga ggaagctgag      600

agaaaagata aggaaaaaca agcagaattt aagaagatta gagaaaaggt gattcctagc      660

gcggaagagt taacagagac tagacgaaaa gcagaagagg ctgaagcaaa agaaccagag      720

cttactaaaa aagtaaaaga agctgaggaa aaagttactg aagccaaaca aaaattggat      780

gctgaacgtg ctaaagaagt tgctcttcaa gccaaaatcg ctgagttgga aaatgaagtt      840

catagactag aaacaaaact caaagagatt gatgaatctg actcagaaga ttatgttaaa      900

gaaggtctcc gtgctcctct tcaatctgaa ttggatgcta agcaagctaa actatcaaaa      960

cttgaagagt tgagtgataa gattgatgag ttagacgctg aaattgcaaa acttgaaaaa     1020

gatgtagaag atttcaaaaa ctcagacggt gaatattctg cattatatct tgaagctgca     1080

gaaaaagatt tagctgctaa aaaagctgaa ttagaaaaaa ctgaagctga cctcaagaaa     1140

gcagttgatg agccagaaaa accagctcca gctccagaaa ctccagcccc agaagcacca     1200

gctgaacaac caaaaccagc gccggctcct caaccagctc ctgcaccata aaaattaaag     1260

catgttaaga acatttgaca ttttaatttc ggcactggcc gtcgttttac aacgtcgtga     1320

ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag     1380

ctggctaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat     1440

ggcgatggct gttttggcgg atgagagaag attttcagcc tgatacagat taaatcagaa     1500

cgcagaagcg gtctgataaa acagaatttg cctggcggca gtagcgcggt ggtcccacct     1560

gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg atggtagtgt ggggtctccc     1620

catgcgagag tagggaactg ccaggcatca aataaaacga aaggctcagt cgaaagactg     1680

ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc ctgagtagga caaatccgcc     1740

gggagcggat ttgaacgttg cgaagcaacg gcccggaggg tggcgggcag gacgcccgcc     1800

ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg acggatggcc tttttgcgtt     1860

tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgggat     1920

ccgtcgacct gcagccaagc ttggcactgg ccgtcgtttt acaacgtcgt gactgggaaa     1980

accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggctaa     2040

tagcgaagag gcccgcaccg atcgcccttc ccaacagttg cgcagcctga atggcgatgg     2100

ctgttttggc ggatgagaga agattttcag cctgatacag attaaatcag aacgcagaag     2160

cggtctgata aaacagaatt tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat     2220

gccgaactca gaagtgaaac gccgtagcgc cgatggtagt gtggggtctc cccatgcgag     2280

agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc     2340

gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg     2400

atttgaacgt tgcgaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg     2460

ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa     2520

ctcttttgtt tatttttcta aatacattca aatatgtatc cgctcatgtt taaaactagt     2580

ccgaggtccc gggggttggg cgtcgcttgg tcggtcattt cgaaccccag agtcccgctc     2640

agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga gcggcgatac     2700

cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca atatcacggg     2760

tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag tcgatgaatc     2820

cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca tgggtcacga     2880

cgagatcctc gccgtcgggc atgcgcgcct tgagcctggc gaacagttcg gctggcgcga     2940

gcccctgatg ctcttcgtcc agatcatctg atcgacaaga cccggctcca tcccgagtac     3000

gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc ggatcaagcg     3060

tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga gcaaggtgag     3120

atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt cccgcttcag     3180

tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac gatagccgcg     3240

ctgcctcgtc ctgcagttca ttcagggcac cggacaggtc ggtcttgaca aaaagaaccg     3300

ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt gtctgttgtg     3360

cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg tgcaatccat     3420

cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcagatctt gatcccctgc     3480

gccatcagat ccttggcggc aagaaagcca tccagtttac tttgcagggc ttcccaacct     3540

taccagaggg cgccccagct ggcaattccg gttcgcttgc tgtccataaa accgcccagt     3600

ctagctatcg ccatcccggg accttctagt agatctgaat tcggtgaggt tatggcggag     3660

ggttgcgagg tctaggagaa cagaggaagt catgctttga agcatataag ctgccctgcc     3720

cctcaaggtt ttcttcaagt gaggttttat ctaactgcct aacggcaggg gaaccgtata     3780

ttgcttacgg tatgagaccc cttaaacgtc cggatagtca ccgctcttct ttagctccgc     3840

gacatgccta gcaaccgtgg cgcgagagac tcctacctct gcccctattt cagcccacgt     3900

gggaactgtc cctgtctgga aatactgatc gttcaccatt tggctaatac gggacttcgt     3960

agatcgtcct tgagcctttt tcttacggtg cgtcttttca agcttcgacc tttgtgcttg     4020

cgcatatttg ccctcggggt ctgttttcca gcgttgtgcg gctttttgtc cgcctctgcg     4080

tcccatcgtg gccaaggctt tccgctcgct gctggtggct ttacctggtg cgttagagcc     4140

gctgtaggtc tcgctcttgg attgggcgac atacccgcgc acgcgccttg ccatggtttg     4200

gcggtcgcgc atgggtggca tctcgttgtc gcggcctgca ccgccgtggg tgtgtgcgac     4260

gttgtaggcg tgctcatagg cgtcgatgat tgctgcgtct gtcaggcgtt ggccttgctg     4320

gcgcaagcgg tggccagtct taagcgcatg tctaaaggct gtttcgtcgc gtgctgcggt     4380

tccttggaca atccagagca cacgcacacc gtcgataagt tccgggtcat actggtcgag     4440

accaccggcg atttccgcgt ctacgtcctg ggcgagtgct ttgaatgctt gggcttcttc     4500

acggcgggtc ttgaccgcgt tgataagttc gcggccagag ctgaattgct ggcgtggggt     4560

ggggttgaac tggtcgtgtc ctgccatatc ccttacctgc tttatcaagt ctccaaggcg     4620

catcacccgg ttgtgctgcc tataccaacg ataagcggta ggggctttgc ctgtgtagaa     4680

cgggttgcgg ctaaagcggt gggaaaagtg cgggtcatgg tctaaaagct cacccagcac     4740

acgcgtggtt gctgcaagaa gcttcatctg cgcagattta ccgttacggt cagcgtagac     4800

agggtcaata agccatatga actgggcttt gccgttagtt gggttaatac ccacccaggc     4860

tggcccgacg ctatgagtaa tcagtgagcg caccacgtcg cggacgtacg ggtttaagtc     4920

tgcggggtca ccgcctgcgg tacctacttg gtcaacgtct acgaccagga cggcggcgta     4980

ctgcttggtg gtgagcatgg cgtactcgca ccgtcctaaa gcatcagtct cgaagcgata     5040

catacgcggc gagttcgtgc cgtcagcgtt gcgtcgatag gcctttttaa agtctcgtgt     5100

gactgaaccg tggagtacat cgcggcctag atgatcgcgt aaaaggtcgc ggtcactggc     5160

agatgctggg gtgttgtcca gtccaccacg gtcgcgctcg acgcgggtag gtgttttagt     5220

gtgcgcattc tgcgcatgag tctgtaaact catgaccgtg atttctccca ggtgtgtgct     5280

gggtgataag cgaaagtcat cgggttgccg cccggtggct ttcttcgttt ttcattgtct     5340

ttccctgact ctaaatgaca ccggtgttat ttactagcca tgacacgcga aaaatatgcc     5400

ttttacctgc ggttacgtat ggctagacat atggcaagct atacgtaacc gcgtttcagc     5460

tgcacagggc tgtctgcgca gatttaccat agatctacta g                         5501


