                         SEQUENCE LISTING

<110>  The Board of Trustees of the Leland Stanford Junior 
       University
       Garcia, Christopher K
       Spangler, Jamie
       Bluestone, Jeffrey
       Trotta, Eleonora
 
<120>  SINGLE-CHAIN IL-2/ANTIBODY FUSIONS THAT SELECTIVELY ACTIVATE

<130>  STAN-1458WO

<150>  62/636,605
<151>  2018-02-28

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  443
<212>  PRT
<213>  Rattus norvegicus

<400>  1

Glu Ile Gln Leu Gln Gln Ser Gly Pro Glu Leu Arg Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Asn Ile Thr Asp Tyr 
            20                  25                  30          


Leu Ile His Trp Val Arg His Arg Pro Glu His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Asp Pro Glu Asp Gly Glu Thr Arg Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Ser Lys Ala Thr Leu Thr Ala Asp Thr Ser Ser Asn Ala Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Pro Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Ser Leu Asp Ser Thr Tyr Ile Tyr Pro Phe Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser Ala Glu Thr Thr Ala Pro Ser 
        115                 120                 125             


Val Tyr Pro Leu Ala Pro Gly Thr Ala Leu Lys Ser Asn Ser Met Val 
    130                 135                 140                 


Thr Leu Gly Cys Leu Val Lys Gly Tyr Phe Pro Glu Pro Val Thr Val 
145                 150                 155                 160 


Thr Trp Asn Ser Gly Ala Leu Ser Ser Gly Val His Thr Phe Pro Ala 
                165                 170                 175     


Val Leu Gln Ser Gly Leu Tyr Thr Leu Thr Ser Ser Val Thr Val Pro 
            180                 185                 190         


Ser Ser Thr Trp Ser Ser Gln Ala Val Thr Cys Asn Val Ala His Pro 
        195                 200                 205             


Ala Ser Ser Thr Lys Val Asp Lys Lys Ile Val Pro Arg Glu Cys Asn 
    210                 215                 220                 


Pro Cys Gly Cys Thr Gly Ser Glu Val Ser Ser Val Phe Ile Phe Pro 
225                 230                 235                 240 


Pro Lys Thr Lys Asp Val Leu Thr Ile Thr Leu Thr Pro Lys Val Thr 
                245                 250                 255     


Cys Val Val Val Asp Ile Ser Gln Asn Asp Pro Glu Val Arg Phe Ser 
            260                 265                 270         


Trp Phe Ile Asp Asp Val Glu Val His Thr Ala Gln Thr His Ala Pro 
        275                 280                 285             


Glu Lys Gln Ser Asn Ser Thr Leu Arg Ser Val Ser Glu Leu Pro Ile 
    290                 295                 300                 


Val His Arg Asp Trp Leu Asn Gly Lys Thr Phe Lys Cys Lys Val Asn 
305                 310                 315                 320 


Ser Gly Ala Phe Pro Ala Pro Ile Glu Lys Ser Ile Ser Lys Pro Glu 
                325                 330                 335     


Gly Thr Pro Arg Gly Pro Gln Val Tyr Thr Met Ala Pro Pro Lys Glu 
            340                 345                 350         


Glu Met Thr Gln Ser Gln Val Ser Ile Thr Cys Met Val Lys Gly Phe 
        355                 360                 365             


Tyr Pro Pro Asp Ile Tyr Thr Glu Trp Lys Met Asn Gly Gln Pro Gln 
    370                 375                 380                 


Glu Asn Tyr Lys Asn Thr Pro Pro Thr Met Asp Thr Asp Gly Ser Tyr 
385                 390                 395                 400 


Phe Leu Tyr Ser Lys Leu Asn Val Lys Lys Glu Thr Trp Gln Gln Gly 
                405                 410                 415     


Asn Thr Phe Thr Cys Ser Val Leu His Glu Gly Leu His Asn His His 
            420                 425                 430         


Thr Glu Lys Ser Leu Ser His Ser Pro Gly Lys 
        435                 440             


<210>  2
<211>  379
<212>  PRT
<213>  Rattus norvegicus

<400>  2

Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala Glu Ala Gln Gln 
1               5                   10                  15      


Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu Glu Gln Leu Leu 
            20                  25                  30          


Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn Tyr Arg Asn Leu 
        35                  40                  45              


Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu Pro Lys Gln Ala 
    50                  55                  60                  


Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu Leu Gly Pro Leu 
65                  70                  75                  80  


Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe Gln Leu Glu Asp 
                85                  90                  95      


Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val Val Lys Leu Lys 
            100                 105                 110         


Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp Glu Ser Ala Thr 
        115                 120                 125             


Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys Gln Ser Ile Ile 
    130                 135                 140                 


Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Val Met Thr Gln Ser Pro Phe Ser Leu Ala Val Ser Glu Gly Glu 
                165                 170                 175     


Met Val Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Ser Ser Gly 
            180                 185                 190         


Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser 
        195                 200                 205             


Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Gly Gln Ser Gly Val Pro 
    210                 215                 220                 


Asp Arg Phe Ile Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
225                 230                 235                 240 


Ser Asp Val Gln Ala Glu Asp Leu Ala Asp Tyr Tyr Cys Leu Gln His 
                245                 250                 255     


Tyr Ile Ser Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            260                 265                 270         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Thr Glu 
        275                 280                 285             


Gln Leu Ala Thr Gly Gly Ala Ser Val Val Cys Leu Met Asn Asn Phe 
    290                 295                 300                 


Tyr Pro Arg Asp Ile Ser Val Lys Trp Lys Ile Asp Gly Thr Glu Arg 
305                 310                 315                 320 


Arg Asp Gly Val Leu Asp Ser Val Thr Asp Gln Asp Ser Lys Asp Ser 
                325                 330                 335     


Thr Tyr Ser Met Ser Ser Thr Leu Ser Leu Thr Lys Ala Asp Tyr Glu 
            340                 345                 350         


Ser His Asn Leu Tyr Thr Cys Glu Val Val His Lys Thr Ser Ser Ser 
        355                 360                 365             


Pro Val Val Lys Ser Phe Asn Arg Asn Glu Cys 
    370                 375                 


<210>  3
<211>  379
<212>  PRT
<213>  Rattus norvegicus

<400>  3

Ala Pro Thr Ser Ser Ser Thr Ser Ser Ser Thr Ala Glu Ala Gln Gln 
1               5                   10                  15      


Gln Gln Gln Gln Gln Gln Gln Gln Gln Gln His Leu Glu Gln Leu Leu 
            20                  25                  30          


Met Asp Leu Gln Glu Leu Leu Ser Arg Met Glu Asn Tyr Arg Asn Leu 
        35                  40                  45              


Lys Leu Pro Arg Met Leu Thr Phe Lys Phe Tyr Leu Pro Lys Gln Ala 
    50                  55                  60                  


Thr Glu Leu Lys Asp Leu Gln Cys Leu Glu Asp Glu Leu Gly Pro Leu 
65                  70                  75                  80  


Arg His Val Leu Asp Leu Thr Gln Ser Lys Ser Phe Gln Leu Glu Asp 
                85                  90                  95      


Ala Glu Asn Phe Ile Ser Asn Ile Arg Val Thr Val Val Lys Leu Lys 
            100                 105                 110         


Gly Ser Asp Asn Thr Phe Glu Cys Gln Phe Asp Asp Glu Ser Ala Thr 
        115                 120                 125             


Val Val Asp Phe Leu Arg Arg Trp Ile Ala Phe Cys Gln Ser Ile Ile 
    130                 135                 140                 


Ser Thr Ser Pro Gln Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Asp 
145                 150                 155                 160 


Ile Val Met Thr Gln Ser Pro Phe Ser Leu Ala Val Ser Glu Gly Glu 
                165                 170                 175     


Met Val Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Ser Ser Gly 
            180                 185                 190         


Asn Gln Lys Asn Ala Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln Ser 
        195                 200                 205             


Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Gly Gln Ser Gly Val Pro 
    210                 215                 220                 


Asp Arg Phe Ile Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile 
225                 230                 235                 240 


Ser Asp Val Gln Ala Glu Asp Leu Ala Asp Tyr Tyr Cys Leu Gln His 
                245                 250                 255     


Ala Ile Ser Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu Lys 
            260                 265                 270         


Arg Ala Asp Ala Ala Pro Thr Val Ser Ile Phe Pro Pro Ser Thr Glu 
        275                 280                 285             


Gln Leu Ala Thr Gly Gly Ala Ser Val Val Cys Leu Met Asn Asn Phe 
    290                 295                 300                 


Tyr Pro Arg Asp Ile Ser Val Lys Trp Lys Ile Asp Gly Thr Glu Arg 
305                 310                 315                 320 


Arg Asp Gly Val Leu Asp Ser Val Thr Asp Gln Asp Ser Lys Asp Ser 
                325                 330                 335     


Thr Tyr Ser Met Ser Ser Thr Leu Ser Leu Thr Lys Ala Asp Tyr Glu 
            340                 345                 350         


Ser His Asn Leu Tyr Thr Cys Glu Val Val His Lys Thr Ser Ser Ser 
        355                 360                 365             


Pro Val Val Lys Ser Phe Asn Arg Asn Glu Cys 
    370                 375                 


<210>  4
<211>  121
<212>  PRT
<213>  Rattus norvegicus

<400>  4

Glu Ile Gln Leu Gln Gln Ser Gly Pro Glu Leu Arg Arg Pro Gly Ser 
1               5                   10                  15      


Ser Val Lys Leu Ser Cys Lys Ala Ser Gly Tyr Asn Ile Thr Asp Tyr 
            20                  25                  30          


Leu Ile His Trp Val Arg His Arg Pro Glu His Gly Leu Glu Trp Ile 
        35                  40                  45              


Gly Trp Ile Asp Pro Glu Asp Gly Glu Thr Arg Tyr Ala Gln Lys Phe 
    50                  55                  60                  


Gln Ser Lys Ala Thr Leu Thr Ala Asp Thr Ser Ser Asn Ala Ala Tyr 
65                  70                  75                  80  


Met Gln Leu Ser Ser Leu Thr Pro Glu Asp Thr Ala Thr Tyr Phe Cys 
                85                  90                  95      


Ala Arg Ser Leu Asp Ser Thr Tyr Ile Tyr Pro Phe Ala Tyr Trp Gly 
            100                 105                 110         


Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120     


<210>  5
<211>  113
<212>  PRT
<213>  Rattus norvegicus

<400>  5

Asp Ile Val Met Thr Gln Ser Pro Phe Ser Leu Ala Val Ser Glu Gly 
1               5                   10                  15      


Glu Met Val Thr Ile Asn Cys Lys Ser Ser Gln Ser Leu Leu Ser Ser 
            20                  25                  30          


Gly Asn Gln Lys Asn Tyr Leu Ala Trp Tyr Gln Gln Lys Pro Gly Gln 
        35                  40                  45              


Ser Pro Lys Leu Leu Ile Tyr Tyr Ala Ser Thr Gly Gln Ser Gly Val 
    50                  55                  60                  


Pro Asp Arg Phe Ile Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr 
65                  70                  75                  80  


Ile Ser Asp Val Gln Ala Glu Asp Leu Ala Asp Tyr Tyr Cys Leu Gln 
                85                  90                  95      


His Tyr Ile Ser Pro Pro Thr Phe Gly Ala Gly Thr Lys Leu Glu Leu 
            100                 105                 110         


Lys 
    


