                         SEQUENCE LISTING

<110>  University of Pittsburgh - Of The Commonwealth System of 
       Higher Education
       Shanks, Robert M.Q.
       Klarlund, Jes K.
 
<120>  DELIVERING BIOLOGICAL DRUGS TO TISSUES

<130>  8123-100458-02

<150>  62/640,859
<151>  2018-03-09

<160>  26    

<170>  PatentIn version 3.5

<210>  1
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  vWF polypeptide

<400>  1

Trp Arg Glu Pro Ser Phe Met Ala Leu Ser 
1               5                   10  


<210>  2
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  vWF polypeptide

<400>  2

Trp Arg Glu Pro Ser Phe Cys Ala Leu Ser 
1               5                   10  


<210>  3
<211>  215
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Clostridium polypeptide

<400>  3

Glu Ile Lys Asp Leu Ser Glu Asn Lys Leu Pro Val Ile Tyr Met His 
1               5                   10                  15      


Val Pro Lys Ser Gly Ala Leu Asn Gln Lys Val Val Phe Tyr Gly Lys 
            20                  25                  30          


Gly Thr Tyr Asp Pro Asp Gly Ser Ile Ala Gly Tyr Gln Trp Asp Phe 
        35                  40                  45              


Gly Asp Gly Ser Asp Phe Ser Ser Glu Gln Asn Pro Ser His Val Tyr 
    50                  55                  60                  


Thr Lys Lys Gly Glu Tyr Thr Val Thr Leu Arg Val Met Asp Ser Ser 
65                  70                  75                  80  


Gly Gln Met Ser Glu Lys Thr Met Lys Ile Lys Ile Thr Asp Pro Val 
                85                  90                  95      


Tyr Pro Ile Gly Thr Glu Lys Glu Pro Asn Asn Ser Lys Glu Thr Ala 
            100                 105                 110         


Ser Gly Pro Ile Val Pro Gly Ile Pro Val Ser Gly Thr Ile Glu Asn 
        115                 120                 125             


Thr Ser Asp Gln Asp Tyr Phe Tyr Phe Asp Val Ile Thr Pro Gly Glu 
    130                 135                 140                 


Val Lys Ile Asp Ile Asn Lys Leu Gly Tyr Gly Gly Ala Thr Trp Val 
145                 150                 155                 160 


Val Tyr Asp Glu Asn Asn Asn Ala Val Ser Tyr Ala Thr Asp Asp Gly 
                165                 170                 175     


Gln Asn Leu Ser Gly Lys Phe Lys Ala Asp Lys Pro Gly Arg Tyr Tyr 
            180                 185                 190         


Ile His Leu Tyr Met Phe Asn Gly Ser Tyr Met Pro Tyr Arg Ile Asn 
        195                 200                 205             


Ile Glu Gly Ser Val Gly Arg 
    210                 215 


<210>  4
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HS-binding polypeptide

<400>  4

Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro Ser 
1               5                   10                  15      


Leu Arg Lys Tyr Lys 
            20      


<210>  5
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HS-binding polypeptide

<400>  5

Lys Arg Lys Lys Lys Gly Lys Gly Leu Gly Lys Lys Arg Asp Pro Cys 
1               5                   10                  15      


Leu Arg Lys Tyr Lys 
            20      


<210>  6
<211>  290
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ConA polypeptide

<400>  6

Met Ala Ile Ser Lys Lys Ser Ser Leu Phe Leu Pro Ile Phe Thr Phe 
1               5                   10                  15      


Ile Thr Met Phe Leu Met Val Val Asn Lys Val Ser Ser Ser Thr His 
            20                  25                  30          


Glu Thr Asn Ala Leu His Phe Met Phe Asn Gln Phe Ser Lys Asp Gln 
        35                  40                  45              


Lys Asp Leu Ile Leu Gln Gly Asp Ala Thr Thr Gly Thr Asp Gly Asn 
    50                  55                  60                  


Leu Glu Leu Thr Arg Val Ser Ser Asn Gly Ser Pro Gln Gly Ser Ser 
65                  70                  75                  80  


Val Gly Arg Ala Leu Phe Tyr Ala Pro Val His Ile Trp Glu Ser Ser 
                85                  90                  95      


Ala Val Val Ala Ser Phe Glu Ala Thr Phe Thr Phe Leu Ile Lys Ser 
            100                 105                 110         


Pro Asp Ser His Pro Ala Asp Gly Ile Ala Phe Phe Ile Ser Asn Ile 
        115                 120                 125             


Asp Ser Ser Ile Pro Ser Gly Ser Thr Gly Arg Leu Leu Gly Leu Phe 
    130                 135                 140                 


Pro Asp Ala Asn Val Ile Arg Asn Ser Thr Thr Ile Asp Phe Asn Ala 
145                 150                 155                 160 


Ala Tyr Asn Ala Asp Thr Ile Val Ala Val Glu Leu Asp Thr Tyr Pro 
                165                 170                 175     


Asn Thr Asp Ile Gly Asp Pro Ser Tyr Pro His Ile Gly Ile Asp Ile 
            180                 185                 190         


Lys Ser Val Arg Ser Lys Lys Thr Ala Lys Trp Asn Met Gln Asn Gly 
        195                 200                 205             


Lys Val Gly Thr Ala His Ile Ile Tyr Asn Ser Val Asp Lys Arg Leu 
    210                 215                 220                 


Ser Ala Val Val Ser Tyr Pro Asn Ala Asp Ser Ala Thr Val Ser Tyr 
225                 230                 235                 240 


Asp Val Asp Leu Asp Asn Val Leu Pro Glu Trp Val Arg Val Gly Leu 
                245                 250                 255     


Ser Ala Ser Thr Gly Leu Tyr Lys Glu Thr Asn Thr Ile Leu Ser Trp 
            260                 265                 270         


Ser Phe Thr Ser Lys Leu Lys Ser Asn Glu Ile Pro Asp Ile Ala Thr 
        275                 280                 285             


Val Val 
    290 


<210>  7
<211>  212
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  WGA polypeptide

<400>  7

Met Lys Met Met Ser Thr Arg Ala Leu Ala Leu Gly Ala Ala Ala Val 
1               5                   10                  15      


Leu Ala Phe Ala Ala Ala Thr Ala Gln Ala Gln Arg Cys Gly Glu Gln 
            20                  25                  30          


Gly Ser Asn Met Glu Cys Pro Asn Asn Leu Cys Cys Ser Gln Tyr Gly 
        35                  40                  45              


Tyr Cys Gly Met Gly Gly Asp Tyr Cys Gly Lys Gly Cys Gln Asn Gly 
    50                  55                  60                  


Ala Cys Trp Thr Ser Lys Arg Cys Gly Ser Gln Ala Gly Gly Ala Thr 
65                  70                  75                  80  


Cys Thr Asn Asn Gln Cys Cys Ser Gln Tyr Gly Tyr Cys Gly Phe Gly 
                85                  90                  95      


Ala Glu Tyr Cys Gly Ala Gly Cys Gln Gly Gly Pro Cys Arg Ala Asp 
            100                 105                 110         


Ile Lys Cys Gly Ser Gln Ala Gly Gly Lys Leu Cys Pro Asn Asn Leu 
        115                 120                 125             


Cys Cys Ser Gln Trp Gly Phe Cys Gly Leu Gly Ser Glu Phe Cys Gly 
    130                 135                 140                 


Gly Gly Cys Gln Ser Gly Ala Cys Ser Thr Asp Lys Pro Cys Gly Lys 
145                 150                 155                 160 


Asp Ala Gly Gly Arg Val Cys Thr Asn Asn Tyr Cys Cys Ser Lys Trp 
                165                 170                 175     


Gly Ser Cys Gly Ile Gly Pro Gly Tyr Cys Gly Ala Gly Cys Gln Ser 
            180                 185                 190         


Gly Gly Cys Asp Gly Val Phe Ala Glu Ala Ile Thr Ala Asn Ser Thr 
        195                 200                 205             


Leu Leu Gln Glu 
    210         


<210>  8
<211>  217
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Jac polypeptide

<400>  8

Met Ala Tyr Ser Ser Leu Leu Ser Leu Ser Val Leu Ala Leu Leu Phe 
1               5                   10                  15      


Ser Ile Ser Ser Ala Asp Thr Arg Lys Trp Phe Leu Ala Asn Gly Ile 
            20                  25                  30          


Asn Gln Asn Pro Ile Gly Ile Ile Glu Ala Ala Val Gly Val Ser Glu 
        35                  40                  45              


Asp Leu Leu Asn Leu Asn Gly Met Glu Ala Lys Asn Asp Glu Gln Ser 
    50                  55                  60                  


Gly Ile Ser Gln Thr Val Ile Val Gly Pro Trp Gly Ala Lys Val Ser 
65                  70                  75                  80  


Thr Ser Ser Asn Gly Lys Ala Phe Asp Asp Gly Ala Phe Thr Gly Ile 
                85                  90                  95      


Arg Glu Ile Asn Leu Ser Tyr Asn Lys Glu Thr Ala Ile Gly Asp Phe 
            100                 105                 110         


Gln Val Val Tyr Asp Leu Asn Gly Ser Pro Tyr Val Gly Gln Asn His 
        115                 120                 125             


Lys Ser Phe Ile Thr Gly Phe Thr Pro Val Lys Ile Ser Leu Asp Phe 
    130                 135                 140                 


Pro Ser Glu Tyr Ile Met Glu Val Ser Gly Tyr Thr Gly Asn Val Ser 
145                 150                 155                 160 


Gly Tyr Val Val Val Arg Ser Leu Thr Phe Lys Thr Asn Lys Lys Thr 
                165                 170                 175     


Tyr Gly Pro Tyr Gly Val Thr Ser Gly Thr Pro Phe Asn Leu Pro Ile 
            180                 185                 190         


Glu Asn Gly Leu Ile Val Gly Phe Lys Gly Ser Ile Gly Tyr Trp Leu 
        195                 200                 205             


Asp Tyr Phe Ser Met Tyr Leu Ser Leu 
    210                 215         


<210>  9
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  9

Gly Gly Gly Gly Ser 
1               5   


<210>  10
<211>  116
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ig-binding polypeptide

<400>  10

Ala Asp Asn Lys Phe Asn Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile 
1               5                   10                  15      


Leu His Leu Pro Asn Leu Asn Glu Glu Gln Arg Asn Gly Phe Ile Gln 
            20                  25                  30          


Ser Leu Lys Asp Asp Pro Ser Gln Ser Ala Asn Leu Leu Ala Glu Ala 
        35                  40                  45              


Lys Lys Leu Asn Asp Ala Gln Ala Pro Lys Ala Asp Asn Lys Phe Asn 
    50                  55                  60                  


Lys Glu Gln Gln Asn Ala Phe Tyr Glu Ile Leu His Leu Pro Asn Leu 
65                  70                  75                  80  


Thr Glu Glu Gln Arg Asn Gly Phe Ile Gln Ser Leu Lys Asp Asp Pro 
                85                  90                  95      


Ser Val Ser Lys Glu Ile Leu Ala Glu Ala Lys Lys Leu Asn Asp Ala 
            100                 105                 110         


Gln Ala Pro Lys 
        115     


<210>  11
<211>  77
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ig-binding polypeptide

<400>  11

Lys Thr Phe Thr Val Thr Glu Lys Pro Glu Val Ile Asp Ala Ser Glu 
1               5                   10                  15      


Leu Thr Pro Ala Val Thr Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr 
            20                  25                  30          


Leu Lys Gly Glu Thr Thr Thr Lys Ala Val Asp Ala Glu Thr Ala Glu 
        35                  40                  45              


Lys Ala Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly Val Trp 
    50                  55                  60                  


Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu 
65                  70                  75          


<210>  12
<211>  161
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  TNF receptor 1 polypeptide

<400>  12

Asp Ser Val Cys Pro Gln Gly Lys Tyr Ile His Pro Gln Asn Asn Ser 
1               5                   10                  15      


Ile Cys Cys Thr Lys Cys His Lys Gly Thr Tyr Leu Tyr Asn Asp Cys 
            20                  25                  30          


Pro Gly Pro Gly Gln Asp Thr Asp Cys Arg Glu Cys Glu Ser Gly Ser 
        35                  40                  45              


Phe Thr Ala Ser Glu Asn His Leu Arg His Cys Leu Ser Cys Ser Lys 
    50                  55                  60                  


Cys Arg Lys Glu Met Gly Gln Val Glu Ile Ser Ser Cys Thr Val Asp 
65                  70                  75                  80  


Arg Asp Thr Val Cys Gly Cys Arg Lys Asn Gln Tyr Arg His Tyr Trp 
                85                  90                  95      


Ser Glu Asn Leu Phe Gln Cys Phe Asn Cys Ser Leu Cys Leu Asn Gly 
            100                 105                 110         


Thr Val His Leu Ser Cys Gln Glu Lys Gln Asn Thr Val Cys Thr Cys 
        115                 120                 125             


His Ala Gly Phe Phe Leu Arg Glu Asn Glu Cys Val Ser Cys Ser Asn 
    130                 135                 140                 


Cys Lys Lys Ser Leu Glu Cys Thr Lys Leu Cys Leu Pro Gln Ile Glu 
145                 150                 155                 160 


Asn 
    


<210>  13
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P144 polypeptide

<400>  13

Thr Ser Leu Asp Ala Ser Ile Ile Trp Ala Met Met Gln Asn 
1               5                   10                  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  P17 polypeptide

<400>  14

Lys Arg Ile Trp Phe Ile Pro Arg Ser Ser Trp Tyr Glu Arg Ala 
1               5                   10                  15  


<210>  15
<211>  176
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary fusion protein

<400>  15

Met Trp Arg Glu Pro Ser Phe Met Ala Leu Ser Ala Ser Gly Gly Gly 
1               5                   10                  15      


Gly Ser Gly Gly Gly Gly Ser Ala Ser Met Gly Thr Pro Ala Val Thr 
            20                  25                  30          


Thr Tyr Lys Leu Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr 
        35                  40                  45              


Thr Lys Ala Val Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys Gln Tyr 
    50                  55                  60                  


Ala Asn Asp Asn Gly Val Asp Gly Val Trp Thr Tyr Asp Asp Ala Thr 
65                  70                  75                  80  


Lys Thr Phe Thr Val Thr Glu Val Asn Thr Pro Ala Val Thr Thr Tyr 
                85                  90                  95      


Lys Leu Val Ile Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Lys 
            100                 105                 110         


Ala Val Asp Ala Glu Thr Ala Glu Lys Ala Phe Lys Gln Tyr Ala Asn 
        115                 120                 125             


Asp Asn Gly Val Asp Gly Val Trp Thr Tyr Asp Asp Ala Thr Lys Thr 
    130                 135                 140                 


Phe Thr Val Thr Glu Ile Gly Glu Asn Leu Tyr Phe Gln Gly Ile Asp 
145                 150                 155                 160 


Glu Asn Leu Tyr Phe Gln Gly Gly Ser His His His His His His His 
                165                 170                 175     


<210>  16
<211>  186
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary fusion protein

<400>  16

Met Ser Lys Lys His His His His His His His His Gly Gly Gly Gly 
1               5                   10                  15      


Ser Ala Ser Met Gly Thr Pro Ala Val Thr Thr Tyr Lys Leu Val Ile 
            20                  25                  30          


Asn Gly Lys Thr Leu Lys Gly Glu Thr Thr Thr Lys Ala Val Asp Ala 
        35                  40                  45              


Glu Thr Ala Glu Lys Ala Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val 
    50                  55                  60                  


Asp Gly Val Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr 
65                  70                  75                  80  


Glu Val Asn Thr Pro Ala Val Thr Thr Tyr Lys Leu Val Ile Asn Gly 
                85                  90                  95      


Lys Thr Leu Lys Gly Glu Thr Thr Thr Lys Ala Val Asp Ala Glu Thr 
            100                 105                 110         


Ala Glu Lys Ala Phe Lys Gln Tyr Ala Asn Asp Asn Gly Val Asp Gly 
        115                 120                 125             


Val Trp Thr Tyr Asp Asp Ala Thr Lys Thr Phe Thr Val Thr Glu Ile 
    130                 135                 140                 


Gly Glu Asn Leu Tyr Phe Gln Gly Ile Asp Glu Asn Leu Tyr Phe Gln 
145                 150                 155                 160 


Gly Gly Ser Ala Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Ala Ser 
                165                 170                 175     


Trp Arg Glu Pro Ser Phe Met Ala Leu Ser 
            180                 185     


<210>  17
<211>  82
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary fusion protein

<400>  17

Met Ser Lys Lys Trp Arg Glu Pro Ser Phe Met Ala Leu Ser Ala Ser 
1               5                   10                  15      


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Ala Ala Ala Lys Thr 
            20                  25                  30          


Ser Leu Asp Ala Ser Ile Ile Trp Ala Met Met Gln Asn Glu Ala Ala 
        35                  40                  45              


Ala Lys Glu Ala Ala Ala Lys Thr Ser Leu Asp Ala Ser Ile Ile Trp 
    50                  55                  60                  


Ala Met Met Gln Asn Gly Gly Gly Gly Ser His His His His His His 
65                  70                  75                  80  


His His 
        


<210>  18
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Exemplary fusion protein

<400>  18

Met Ser Lys Lys Trp Arg Glu Pro Ser Phe Met Ala Leu Ser Ala Ser 
1               5                   10                  15      


Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Lys 
            20                  25                  30          


Arg Ile Trp Phe Ile Pro Arg Ser Ser Trp Tyr Glu Arg Ala Gly Gly 
        35                  40                  45              


Gly Gly Ser Gly Gly Gly Gly Ser Lys Arg Ile Trp Phe Ile Pro Arg 
    50                  55                  60                  


Ser Ser Trp Tyr Glu Arg Ala Gly Gly Gly Gly Ser His His His His 
65                  70                  75                  80  


His His His His 
                


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  19

Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10  


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Collagen-binding anchor domain from the von Willebrand factor

<400>  20

Tyr Arg Glu Pro Ser Phe Met Ala Leu Ser 
1               5                   10  


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Collagen-binding anchor domain from the von Willebrand factor

<400>  21

Trp Lys Glu Pro Ser Phe Met Ala Leu Ser 
1               5                   10  


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Collagen-binding anchor domain from the von Willebrand factor

<400>  22

Trp Arg Asp Pro Ser Phe Met Ala Leu Ser 
1               5                   10  


<210>  23
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Collagen-binding anchor domain from the von Willebrand factor

<400>  23

Trp Arg Glu Ala Ser Phe Met Ala Leu Ser 
1               5                   10  


<210>  24
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Collagen-binding anchor domain from the von Willebrand factor

<400>  24

Trp Arg Glu Pro Thr Phe Met Ala Leu Ser 
1               5                   10  


<210>  25
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Collagen-binding anchor domain from the von Willebrand factor

<400>  25

Trp Arg Glu Pro Ser Tyr Met Ala Leu Ser 
1               5                   10  


<210>  26
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Collagen-binding anchor domain from the von Willebrand factor

<400>  26

Trp Arg Glu Pro Ser Phe Ala Ala Leu Ser 
1               5                   10  


