                         SEQUENCE LISTING

<110>  The General Hospital Corporation
 
<120>  COXIELLA BURNETII EPITOPES FOR T CELL-TARGETED Q FEVER VACCINES

<130>  29539-0389WO1

<140>  PCT/US2019/023878
<141>  2019-03-25

<150>  US 62/647,156
<151>  2018-03-23

<160>  59    

<170>  PatentIn version 3.5

<210>  1
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  1

Ser Glu Gln Ile Thr Leu Gln Thr Ala Glu Lys Val Gly Leu Asn Val 
1               5                   10                  15      


Ala 
    


<210>  2
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  2

Thr Pro Thr Phe Val Ile Gly Asn Lys Ala Leu Thr Lys Phe Gly Phe 
1               5                   10                  15      


<210>  3
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  3

Lys Asp Asp Ile Leu Glu Ala Val Ala Asn Met Ser Val Met Asp Val 
1               5                   10                  15      


<210>  4
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  4

Lys Ile Gly Val Ile Lys Ala Ile Arg Thr Ile Thr Gly Leu Gly Leu 
1               5                   10                  15      


Lys Glu Ala 
            


<210>  5
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  5

Leu Ala Gln Tyr Arg Glu Leu Glu Ala Phe Ser Gln Phe Ala Ser 
1               5                   10                  15  


<210>  6
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  6

Ser His Glu Val Leu His Ala Met Ser Arg Gly Val Glu Val Leu Ala 
1               5                   10                  15      


<210>  7
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  7

Ser Arg Ala Phe Leu Thr Ala Asn Lys Asn Lys Pro Gly Val Lys Thr 
1               5                   10                  15      


<210>  8
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  8

Ile Lys Gly Trp Gln Glu Ala Leu Thr Arg Met Lys Pro Gly Ala Ile 
1               5                   10                  15      


Trp Glu Ile 
            


<210>  9
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  9

Ala Ile Tyr Phe Ile Gly Trp Tyr Ala Asn Leu Ala His Ile Lys Leu 
1               5                   10                  15      


Gly Ile Ser 
            


<210>  10
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  10

Glu His Thr Ile Val Val Asn Ala Ser Ala Ser Glu Ala Ala Ala Leu 
1               5                   10                  15      


Gln 
    


<210>  11
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  11

Pro Ile Thr Lys Lys Gln Leu Lys Thr Met Ser Asn Tyr Glu Val Ile 
1               5                   10                  15      


Ala Lys 
        


<210>  12
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  12

Gly Lys His Phe Asp Gly Ile Lys Val Leu Lys Leu Ser Pro Gln Asn 
1               5                   10                  15      


Thr Ile 
        


<210>  13
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  13

Phe Thr Phe Arg Ser Gln Asp Ser Arg Arg Val Gln Glu Trp 
1               5                   10                  


<210>  14
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  14

Pro Asp Tyr Val Leu Asn Ala Val Asn His Ile Arg Tyr Lys Pro 
1               5                   10                  15  


<210>  15
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  15

Met Met Glu His Leu Gln Asn Ile Thr Asn Leu Val Ser Thr Gly Arg 
1               5                   10                  15      


Gln Gly Ala 
            


<210>  16
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  16

Leu Lys Pro Phe His Phe Ile Ser Ser Pro Thr Arg Asp Leu Gln Ile 
1               5                   10                  15      


Ala 
    


<210>  17
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  17

Lys Ile Pro Val Lys Ile Ile Lys Pro Pro Phe Val Arg Arg Gly 
1               5                   10                  15  


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  18

Gln Gly His Ile Ile Asn Ile Gly Ser Ile Ser Ser His Gln Val 
1               5                   10                  15  


<210>  19
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  19

Glu Ala Val Tyr Lys Gly Phe Thr Pro Leu Lys Ala Glu Asp Ile Ala 
1               5                   10                  15      


Glu Ala 
        


<210>  20
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  20

Ala Gln Pro Ile Ile His Arg Leu Ser Thr Gly Gln Asn Thr Asn Pro 
1               5                   10                  15      


<210>  21
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  21

Ile Ala Arg Tyr Phe Met Val Asn Ile Ser Gln Leu Ile Gly Glu Glu 
1               5                   10                  15      


<210>  22
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  22

Arg Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val Glu Ala Leu 
1               5                   10                  15      


Lys Arg Phe 
            


<210>  23
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  23

Arg Glu Ala Val Leu Phe Leu Val Thr Ile Lys Glu Leu Leu Glu Asp 
1               5                   10                  15      


Pro 
    


<210>  24
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  24

Leu Pro Pro Val Thr Ser Ser Val Ala Val Lys Val Pro Ser Ser 
1               5                   10                  15  


<210>  25
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  25

Ser Asp Met Trp Gln Ala Leu Leu Ala Gly Lys Ser Gly Val Lys 
1               5                   10                  15  


<210>  26
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  26

Gln Thr Gln Leu Gln Gln Ser Phe Ser Lys Arg Thr Met Ala Thr 
1               5                   10                  15  


<210>  27
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  27

Arg Phe Asp Leu Ser Leu Met Leu Asn Tyr Pro Asn Ser Ala Asp Arg 
1               5                   10                  15      


Tyr 
    


<210>  28
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  28

Ile Ser Leu Leu Val Phe Lys Asn Ser His Arg Val Gln Leu Trp Ala 
1               5                   10                  15      


Lys 
    


<210>  29
<211>  25
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  29

Val Ala Arg Val Ser Arg Leu Lys Asp Asn Phe Val Val Leu Glu Ile 
1               5                   10                  15      


Ser Lys Gly Thr Glu Ile Thr Val Gln 
            20                  25  


<210>  30
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  30

Gly Thr Glu Ile Thr Val Gln Lys Ala Ser Ile Ala Ser Val Leu Pro 
1               5                   10                  15      


Lys 
    


<210>  31
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  31

Ala Glu Asn Val Leu Ile Ile His Asn Lys Thr Leu Ala His Arg Tyr 
1               5                   10                  15      


Leu Ala 
        


<210>  32
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  32

Thr Gly Glu Ile Val Lys Met Ile Asn Gln Ala Lys Gln Ser Ile Tyr 
1               5                   10                  15      


Val Gln Gly 
            


<210>  33
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  33

Asp Gly Arg Leu Glu Gln Leu Asn Ser Gln Asn Gln Gln Leu Gln 
1               5                   10                  15  


<210>  34
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  34

Pro Ala Lys Ile Asn Leu Ala Arg Thr Tyr Ile Ala Met Glu Asp 
1               5                   10                  15  


<210>  35
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  35

Val Phe Asn Ile Thr Leu Gln Lys Val Met Ala Pro Glu Leu Pro Val 
1               5                   10                  15      


Leu 
    


<210>  36
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  36

Ile Asp His Leu Gln Gln Met Thr Arg Gln Gln Val Ala Met Gln Thr 
1               5                   10                  15      


His Lys 
        


<210>  37
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  37

Val Ala Lys Leu Arg Gly Asp Leu Ser Ser Ile Ile His Lys Leu 
1               5                   10                  15  


<210>  38
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  38

Leu Ser Ser Ile Ile His Lys Leu Thr Ser Phe Ser Lys Thr Glu Ala 
1               5                   10                  15      


<210>  39
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  39

Gln Glu Leu Phe Val Ala Gln Asn Lys Ala Met Ser Asp Phe Met 
1               5                   10                  15  


<210>  40
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  40

Gln Asn Ala Phe Gln Leu Gln Glu Thr Ile Val Ser Thr Glu Asn 
1               5                   10                  15  


<210>  41
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  41

Leu Lys Asp Val Val Ala Leu Arg Asn Gln Ala Gln Thr Ala Lys 
1               5                   10                  15  


<210>  42
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  42

Asp His Ala Tyr Lys Leu Ala Val Ser Ser Thr Lys Ser Met Thr 
1               5                   10                  15  


<210>  43
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  43

Asn Ala Gly Ile Ile Arg Asn Lys Leu Lys Ile Gln Ala Thr Ile Asn 
1               5                   10                  15      


<210>  44
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  44

Gly Leu Ser Trp Leu Thr Ile Leu Lys Lys Arg Asn Asn Tyr Arg Asp 
1               5                   10                  15      


Ser Phe Asn 
            


<210>  45
<211>  17
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  45

Gly Val Ala Tyr Thr Tyr Asn Arg Ala Asn Ala Gly Leu Pro Thr Asn 
1               5                   10                  15      


Lys 
    


<210>  46
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  46

Val Pro Gly Tyr Arg Asn Ala Ser Ser Lys Arg Phe Val Ala Pro 
1               5                   10                  15  


<210>  47
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  47

Lys Ala Gln Leu Ile Gln Leu Lys Thr His Val Thr Ile Asn Ala Thr 
1               5                   10                  15      


<210>  48
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  48

Ser Pro Ala Val Leu Ser Ala Ala Lys Lys Ile Phe Gly Asp Gly Ala 
1               5                   10                  15      


<210>  49
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  49

Asp Gln Arg Ile Thr Gln Leu Lys Asn Leu Asn Ser Asn Asn Ser Asp 
1               5                   10                  15      


Ser Ser Asn Asp Asn 
            20      


<210>  50
<211>  18
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  HLA class II epitope

<400>  50

Leu Arg Pro Val Arg Tyr Phe Thr Gly Val Pro Ser Pro Val Lys Thr 
1               5                   10                  15      


Pro Glu 
        


<210>  51
<211>  625
<212>  PRT
<213>  Mycobacterium tuberculosis

<400>  51

Met Ala Arg Ala Val Gly Ile Asp Leu Gly Thr Thr Asn Ser Val Val 
1               5                   10                  15      


Ser Val Leu Glu Gly Gly Asp Pro Val Val Val Ala Asn Ser Glu Gly 
            20                  25                  30          


Ser Arg Thr Thr Pro Ser Ile Val Ala Phe Ala Arg Asn Gly Glu Val 
        35                  40                  45              


Leu Val Gly Gln Pro Ala Lys Asn Gln Ala Val Thr Asn Val Asp Arg 
    50                  55                  60                  


Thr Val Arg Ser Val Lys Arg His Met Gly Ser Asp Trp Ser Ile Glu 
65                  70                  75                  80  


Ile Asp Gly Lys Lys Tyr Thr Ala Pro Glu Ile Ser Ala Arg Ile Leu 
                85                  90                  95      


Met Lys Leu Lys Arg Asp Ala Glu Ala Tyr Leu Gly Glu Asp Ile Thr 
            100                 105                 110         


Asp Ala Val Ile Thr Thr Pro Ala Tyr Phe Asn Asp Ala Gln Arg Gln 
        115                 120                 125             


Ala Thr Lys Asp Ala Gly Gln Ile Ala Gly Leu Asn Val Leu Arg Ile 
    130                 135                 140                 


Val Asn Glu Pro Thr Ala Ala Ala Leu Ala Tyr Gly Leu Asp Lys Gly 
145                 150                 155                 160 


Glu Lys Glu Gln Arg Ile Leu Val Phe Asp Leu Gly Gly Gly Thr Phe 
                165                 170                 175     


Asp Val Ser Leu Leu Glu Ile Gly Glu Gly Val Val Glu Val Arg Ala 
            180                 185                 190         


Thr Ser Gly Asp Asn His Leu Gly Gly Asp Asp Trp Asp Gln Arg Val 
        195                 200                 205             


Val Asp Trp Leu Val Asp Lys Phe Lys Gly Thr Ser Gly Ile Asp Leu 
    210                 215                 220                 


Thr Lys Asp Lys Met Ala Met Gln Arg Leu Arg Glu Ala Ala Glu Lys 
225                 230                 235                 240 


Ala Lys Ile Glu Leu Ser Ser Ser Gln Ser Thr Ser Ile Asn Leu Pro 
                245                 250                 255     


Tyr Ile Thr Val Asp Ala Asp Lys Asn Pro Leu Phe Leu Asp Glu Gln 
            260                 265                 270         


Leu Thr Arg Ala Glu Phe Gln Arg Ile Thr Gln Asp Leu Leu Asp Arg 
        275                 280                 285             


Thr Arg Lys Pro Phe Gln Ser Val Ile Ala Asp Thr Gly Ile Ser Val 
    290                 295                 300                 


Ser Glu Ile Asp His Val Val Leu Val Gly Gly Ser Thr Arg Met Pro 
305                 310                 315                 320 


Ala Val Thr Asp Leu Val Lys Glu Leu Thr Gly Gly Lys Glu Pro Asn 
                325                 330                 335     


Lys Gly Val Asn Pro Asp Glu Val Val Ala Val Gly Ala Ala Leu Gln 
            340                 345                 350         


Ala Gly Val Leu Lys Gly Glu Val Lys Asp Val Leu Leu Leu Asp Val 
        355                 360                 365             


Thr Pro Leu Ser Leu Gly Ile Glu Thr Lys Gly Gly Phe Met Thr Arg 
    370                 375                 380                 


Leu Ile Glu Arg Asn Thr Thr Ile Pro Thr Lys Arg Ser Glu Thr Phe 
385                 390                 395                 400 


Thr Thr Ala Asp Asp Asn Gln Pro Ser Val Gln Ile Gln Val Tyr Gln 
                405                 410                 415     


Gly Glu Arg Glu Ile Ala Ala His Asn Lys Leu Leu Gly Ser Phe Glu 
            420                 425                 430         


Leu Thr Gly Ile Pro Pro Ala Pro Arg Gly Ile Pro Gln Ile Glu Val 
        435                 440                 445             


Thr Phe Asp Ile Asp Ala Asn Gly Ile Val His Val Thr Ala Lys Asp 
    450                 455                 460                 


Lys Gly Thr Gly Lys Glu Asn Thr Ile Arg Ile Gln Glu Gly Ser Gly 
465                 470                 475                 480 


Leu Ser Lys Glu Asp Ile Asp Arg Met Ile Lys Asp Ala Glu Ala His 
                485                 490                 495     


Ala Glu Glu Asp Arg Lys Arg Arg Glu Glu Ala Asp Val Arg Asn Gln 
            500                 505                 510         


Ala Glu Thr Leu Val Tyr Gln Thr Glu Lys Phe Val Lys Glu Gln Arg 
        515                 520                 525             


Glu Ala Glu Gly Gly Ser Lys Val Pro Glu Asp Thr Leu Asn Lys Val 
    530                 535                 540                 


Asp Ala Ala Val Ala Glu Ala Lys Ala Ala Leu Gly Gly Ser Asp Ile 
545                 550                 555                 560 


Ser Ala Ile Lys Ser Ala Met Glu Lys Leu Gly Gln Glu Ser Gln Ala 
                565                 570                 575     


Leu Gly Gln Ala Ile Tyr Glu Ala Ala Gln Ala Ala Ser Gln Ala Thr 
            580                 585                 590         


Gly Ala Ala His Pro Gly Gly Glu Pro Gly Gly Ala His Pro Gly Ser 
        595                 600                 605             


Ala Asp Asp Val Val Asp Ala Glu Val Val Asp Asp Gly Arg Glu Ala 
    610                 615                 620                 


Lys 
625 


<210>  52
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  52

Gly Pro Gly Pro Gly 
1               5   


<210>  53
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  53

Gly Gly Gly Ser 
1               


<210>  54
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linker

<400>  54

Gly Ser Gly Ser 
1               


<210>  55
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  linkers that include cleavage sequences

<400>  55

Arg Val Lys Arg 
1               


<210>  56
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  CpG oligodeoxynucleotide

<400>  56
tccatgacgt tcctgacgtt                                                   20


<210>  57
<211>  296
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  18-tope concatemer

<400>  57

Arg Phe Asp Leu Ser Leu Met Leu Asn Tyr Pro Asn Ser Ala Asp Arg 
1               5                   10                  15      


Tyr Gly Thr Glu Ile Thr Val Gln Lys Ala Ser Ile Ala Ser Val Leu 
            20                  25                  30          


Pro Lys Asn Ala Gly Ile Ile Arg Asn Lys Leu Lys Ile Gln Ala Thr 
        35                  40                  45              


Ile Asn Pro Asp Tyr Val Leu Asn Ala Val Asn His Ile Arg Tyr Lys 
    50                  55                  60                  


Pro Gln Thr Gln Leu Gln Gln Ser Phe Ser Lys Arg Thr Met Ala Thr 
65                  70                  75                  80  


Gln Gly His Ile Ile Asn Ile Gly Ser Ile Ser Ser His Gln Val Lys 
                85                  90                  95      


Ile Pro Val Lys Ile Ile Lys Pro Pro Phe Val Arg Arg Gly Lys Ile 
            100                 105                 110         


Gly Val Ile Lys Ala Ile Arg Thr Ile Thr Gly Leu Gly Leu Lys Glu 
        115                 120                 125             


Ala Arg Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val Glu Ala 
    130                 135                 140                 


Leu Lys Arg Phe Val Ala Lys Leu Arg Gly Asp Leu Ser Ser Ile Ile 
145                 150                 155                 160 


His Lys Leu Met Met Glu His Leu Gln Asn Ile Thr Asn Leu Val Ser 
                165                 170                 175     


Thr Gly Arg Gln Gly Ala Gly Val Ala Tyr Thr Tyr Asn Arg Ala Asn 
            180                 185                 190         


Ala Gly Leu Pro Thr Asn Lys Leu Ser Ser Ile Ile His Lys Leu Thr 
        195                 200                 205             


Ser Phe Ser Lys Thr Glu Ala Ala Gln Pro Ile Ile His Arg Leu Ser 
    210                 215                 220                 


Thr Gly Gln Asn Thr Asn Pro Ile Ala Arg Tyr Phe Met Val Asn Ile 
225                 230                 235                 240 


Ser Gln Leu Ile Gly Glu Glu Ser Pro Ala Val Leu Ser Ala Ala Lys 
                245                 250                 255     


Lys Ile Phe Gly Asp Gly Ala Gly Lys His Phe Asp Gly Ile Lys Val 
            260                 265                 270         


Leu Lys Leu Ser Pro Gln Asn Thr Ile Asp His Ala Tyr Lys Leu Ala 
        275                 280                 285             


Val Ser Ser Thr Lys Ser Met Thr 
    290                 295     


<210>  58
<211>  445
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  27-tope concatemer

<400>  58

Ile Ala Arg Tyr Phe Met Val Asn Ile Ser Gln Leu Ile Gly Glu Glu 
1               5                   10                  15      


Leu Ser Ser Ile Ile His Lys Leu Thr Ser Phe Ser Lys Thr Glu Ala 
            20                  25                  30          


Asp His Ala Tyr Lys Leu Ala Val Ser Ser Thr Lys Ser Met Thr Arg 
        35                  40                  45              


Phe Asp Leu Ser Leu Met Leu Asn Tyr Pro Asn Ser Ala Asp Arg Tyr 
    50                  55                  60                  


Gly Thr Glu Ile Thr Val Gln Lys Ala Ser Ile Ala Ser Val Leu Pro 
65                  70                  75                  80  


Lys Asn Ala Gly Ile Ile Arg Asn Lys Leu Lys Ile Gln Ala Thr Ile 
                85                  90                  95      


Asn Leu Arg Pro Val Arg Tyr Phe Thr Gly Val Pro Ser Pro Val Lys 
            100                 105                 110         


Thr Pro Glu Leu Pro Pro Val Thr Ser Ser Val Ala Val Lys Val Pro 
        115                 120                 125             


Ser Ser Gln Gly His Ile Ile Asn Ile Gly Ser Ile Ser Ser His Gln 
    130                 135                 140                 


Val Val Pro Gly Tyr Arg Asn Ala Ser Ser Lys Arg Phe Val Ala Pro 
145                 150                 155                 160 


Glu Ala Val Tyr Lys Gly Phe Thr Pro Leu Lys Ala Glu Asp Ile Ala 
                165                 170                 175     


Glu Ala Ser His Glu Val Leu His Ala Met Ser Arg Gly Val Glu Val 
            180                 185                 190         


Leu Ala Lys Ile Pro Val Lys Ile Ile Lys Pro Pro Phe Val Arg Arg 
        195                 200                 205             


Gly Gln Thr Gln Leu Gln Gln Ser Phe Ser Lys Arg Thr Met Ala Thr 
    210                 215                 220                 


Lys Ile Gly Val Ile Lys Ala Ile Arg Thr Ile Thr Gly Leu Gly Leu 
225                 230                 235                 240 


Lys Glu Ala Ala Glu Asn Val Leu Ile Ile His Asn Lys Thr Leu Ala 
                245                 250                 255     


His Arg Tyr Leu Ala Val Ala Lys Leu Arg Gly Asp Leu Ser Ser Ile 
            260                 265                 270         


Ile His Lys Leu Arg Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val 
        275                 280                 285             


Val Glu Ala Leu Lys Arg Phe Gly Val Ala Tyr Thr Tyr Asn Arg Ala 
    290                 295                 300                 


Asn Ala Gly Leu Pro Thr Asn Lys Met Met Glu His Leu Gln Asn Ile 
305                 310                 315                 320 


Thr Asn Leu Val Ser Thr Gly Arg Gln Gly Ala Arg Glu Ala Val Leu 
                325                 330                 335     


Phe Leu Val Thr Ile Lys Glu Leu Leu Glu Asp Pro Ser Pro Ala Val 
            340                 345                 350         


Leu Ser Ala Ala Lys Lys Ile Phe Gly Asp Gly Ala Thr Pro Thr Phe 
        355                 360                 365             


Val Ile Gly Asn Lys Ala Leu Thr Lys Phe Gly Phe Lys Ala Gln Leu 
    370                 375                 380                 


Ile Gln Leu Lys Thr His Val Thr Ile Asn Ala Thr Ala Gln Pro Ile 
385                 390                 395                 400 


Ile His Arg Leu Ser Thr Gly Gln Asn Thr Asn Pro Gly Lys His Phe 
                405                 410                 415     


Asp Gly Ile Lys Val Leu Lys Leu Ser Pro Gln Asn Thr Ile Pro Asp 
            420                 425                 430         


Tyr Val Leu Asn Ala Val Asn His Ile Arg Tyr Lys Pro 
        435                 440                 445 


<210>  59
<211>  734
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  44-tope concatemer

<400>  59

Leu Lys Pro Phe His Phe Ile Ser Ser Pro Thr Arg Asp Leu Gln Ile 
1               5                   10                  15      


Ala Leu Pro Pro Val Thr Ser Ser Val Ala Val Lys Val Pro Ser Ser 
            20                  25                  30          


Arg Phe Asp Leu Ser Leu Met Leu Asn Tyr Pro Asn Ser Ala Asp Arg 
        35                  40                  45              


Tyr Gly Thr Glu Ile Thr Val Gln Lys Ala Ser Ile Ala Ser Val Leu 
    50                  55                  60                  


Pro Lys Asn Ala Gly Ile Ile Arg Asn Lys Leu Lys Ile Gln Ala Thr 
65                  70                  75                  80  


Ile Asn Leu Arg Pro Val Arg Tyr Phe Thr Gly Val Pro Ser Pro Val 
                85                  90                  95      


Lys Thr Pro Glu Ser Glu Gln Ile Thr Leu Gln Thr Ala Glu Lys Val 
            100                 105                 110         


Gly Leu Asn Val Ala Leu Lys Asp Val Val Ala Leu Arg Asn Gln Ala 
        115                 120                 125             


Gln Thr Ala Lys Gln Gly His Ile Ile Asn Ile Gly Ser Ile Ser Ser 
    130                 135                 140                 


His Gln Val Val Pro Gly Tyr Arg Asn Ala Ser Ser Lys Arg Phe Val 
145                 150                 155                 160 


Ala Pro Leu Ala Gln Tyr Arg Glu Leu Glu Ala Phe Ser Gln Phe Ala 
                165                 170                 175     


Ser Gln Thr Gln Leu Gln Gln Ser Phe Ser Lys Arg Thr Met Ala Thr 
            180                 185                 190         


Glu Ala Val Tyr Lys Gly Phe Thr Pro Leu Lys Ala Glu Asp Ile Ala 
        195                 200                 205             


Glu Ala Ser His Glu Val Leu His Ala Met Ser Arg Gly Val Glu Val 
    210                 215                 220                 


Leu Ala Gly Lys His Phe Asp Gly Ile Lys Val Leu Lys Leu Ser Pro 
225                 230                 235                 240 


Gln Asn Thr Ile Ser Asp Met Trp Gln Ala Leu Leu Ala Gly Lys Ser 
                245                 250                 255     


Gly Val Lys Glu His Thr Ile Val Val Asn Ala Ser Ala Ser Glu Ala 
            260                 265                 270         


Ala Ala Leu Gln Ile Ser Leu Leu Val Phe Lys Asn Ser His Arg Val 
        275                 280                 285             


Gln Leu Trp Ala Lys Gln Glu Leu Phe Val Ala Gln Asn Lys Ala Met 
    290                 295                 300                 


Ser Asp Phe Met Phe Thr Phe Arg Ser Gln Asp Ser Arg Arg Val Gln 
305                 310                 315                 320 


Glu Trp Ile Lys Gly Trp Gln Glu Ala Leu Thr Arg Met Lys Pro Gly 
                325                 330                 335     


Ala Ile Trp Glu Ile Ala Glu Asn Val Leu Ile Ile His Asn Lys Thr 
            340                 345                 350         


Leu Ala His Arg Tyr Leu Ala Val Ala Arg Val Ser Arg Leu Lys Asp 
        355                 360                 365             


Asn Phe Val Val Leu Glu Ile Ser Lys Gly Thr Glu Ile Thr Val Gln 
    370                 375                 380                 


Lys Ile Pro Val Lys Ile Ile Lys Pro Pro Phe Val Arg Arg Gly Lys 
385                 390                 395                 400 


Ile Gly Val Ile Lys Ala Ile Arg Thr Ile Thr Gly Leu Gly Leu Lys 
                405                 410                 415     


Glu Ala Asp His Ala Tyr Lys Leu Ala Val Ser Ser Thr Lys Ser Met 
            420                 425                 430         


Thr Arg Leu Gly Phe Met Ser Phe Phe Thr Lys Ala Val Val Glu Ala 
        435                 440                 445             


Leu Lys Arg Phe Val Ala Lys Leu Arg Gly Asp Leu Ser Ser Ile Ile 
    450                 455                 460                 


His Lys Leu Asp Gly Arg Leu Glu Gln Leu Asn Ser Gln Asn Gln Gln 
465                 470                 475                 480 


Leu Gln Met Met Glu His Leu Gln Asn Ile Thr Asn Leu Val Ser Thr 
                485                 490                 495     


Gly Arg Gln Gly Ala Lys Asp Asp Ile Leu Glu Ala Val Ala Asn Met 
            500                 505                 510         


Ser Val Met Asp Val Thr Gly Glu Ile Val Lys Met Ile Asn Gln Ala 
        515                 520                 525             


Lys Gln Ser Ile Tyr Val Gln Gly Ser Arg Ala Phe Leu Thr Ala Asn 
    530                 535                 540                 


Lys Asn Lys Pro Gly Val Lys Thr Arg Glu Ala Val Leu Phe Leu Val 
545                 550                 555                 560 


Thr Ile Lys Glu Leu Leu Glu Asp Pro Ser Pro Ala Val Leu Ser Ala 
                565                 570                 575     


Ala Lys Lys Ile Phe Gly Asp Gly Ala Thr Pro Thr Phe Val Ile Gly 
            580                 585                 590         


Asn Lys Ala Leu Thr Lys Phe Gly Phe Ile Asp His Leu Gln Gln Met 
        595                 600                 605             


Thr Arg Gln Gln Val Ala Met Gln Thr His Lys Gly Val Ala Tyr Thr 
    610                 615                 620                 


Tyr Asn Arg Ala Asn Ala Gly Leu Pro Thr Asn Lys Leu Ser Ser Ile 
625                 630                 635                 640 


Ile His Lys Leu Thr Ser Phe Ser Lys Thr Glu Ala Ala Gln Pro Ile 
                645                 650                 655     


Ile His Arg Leu Ser Thr Gly Gln Asn Thr Asn Pro Lys Ala Gln Leu 
            660                 665                 670         


Ile Gln Leu Lys Thr His Val Thr Ile Asn Ala Thr Pro Asp Tyr Val 
        675                 680                 685             


Leu Asn Ala Val Asn His Ile Arg Tyr Lys Pro Gly Leu Ser Trp Leu 
    690                 695                 700                 


Thr Ile Leu Lys Lys Arg Asn Asn Tyr Arg Asp Ser Phe Asn Ile Ala 
705                 710                 715                 720 


Arg Tyr Phe Met Val Asn Ile Ser Gln Leu Ile Gly Glu Glu 
                725                 730                 


