                         SEQUENCE LISTING

<110>  Firmenich SA
        
 
<120>  Method for producing Vanillin

<130>  10790/PCT

<160>  25    

<170>  PatentIn version 3.5

<210>  1
<211>  1437
<212>  DNA
<213>  Isoeugenol monooxygenase of P. nitroreducens Jin1 (codon modified)


<220>
<221>  CDS
<222>  (1)..(1437)

<400>  1
atg gca cgt ctg aac cgt aat gat ccg caa ctc gtc ggc acc ctg tta         48
Met Ala Arg Leu Asn Arg Asn Asp Pro Gln Leu Val Gly Thr Leu Leu           
1               5                   10                  15                

cca acc cgt att gag gct gac ctg ttc gac ctg gaa gtg gac ggc gaa         96
Pro Thr Arg Ile Glu Ala Asp Leu Phe Asp Leu Glu Val Asp Gly Glu           
            20                  25                  30                    

att ccg aag tcc atc aac ggt acc ttc tac cgt aac acg ccg gag cct        144
Ile Pro Lys Ser Ile Asn Gly Thr Phe Tyr Arg Asn Thr Pro Glu Pro           
        35                  40                  45                        

cag gtg acg ccg cag aaa ttc cac acc ttc atc gat ggt gac ggc atg        192
Gln Val Thr Pro Gln Lys Phe His Thr Phe Ile Asp Gly Asp Gly Met           
    50                  55                  60                            

gcg tct gca ttt cat ttt gaa gat ggc cac gtg gac ttc atc agc cgc        240
Ala Ser Ala Phe His Phe Glu Asp Gly His Val Asp Phe Ile Ser Arg           
65                  70                  75                  80            

tgg gtt aaa acc gcg cgt ttc acg gcg gag aga ctg gca cgt aaa agc        288
Trp Val Lys Thr Ala Arg Phe Thr Ala Glu Arg Leu Ala Arg Lys Ser           
                85                  90                  95                

ctg ttc ggt atg tac cgt aat ccg tac acc gat gac acg tct gtg aag        336
Leu Phe Gly Met Tyr Arg Asn Pro Tyr Thr Asp Asp Thr Ser Val Lys           
            100                 105                 110                   

ggt ctg gat cgt acc gtt gcc aac acg agc atc atc agc cat cac ggt        384
Gly Leu Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Ser His His Gly           
        115                 120                 125                       

aag gtt ctg gcg gtg aaa gaa gat ggc ttg ccg tac gag ctg gac cca        432
Lys Val Leu Ala Val Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro           
    130                 135                 140                           

cgc act ctg gaa acc cgt ggt cgc ttt gac tat gat ggc caa gtc acc        480
Arg Thr Leu Glu Thr Arg Gly Arg Phe Asp Tyr Asp Gly Gln Val Thr           
145                 150                 155                 160           

agc cag acg cac acc gcg cat ccg aag tat gac ccg gaa acc ggt gac        528
Ser Gln Thr His Thr Ala His Pro Lys Tyr Asp Pro Glu Thr Gly Asp           
                165                 170                 175               

ctg ttg ttc ttt ggc tcc gca gcc aag ggt gag gca acg cct gat atg        576
Leu Leu Phe Phe Gly Ser Ala Ala Lys Gly Glu Ala Thr Pro Asp Met           
            180                 185                 190                   

gcc tat tac atc gtt gat aaa cat ggt aag gta acg cat gag act tgg        624
Ala Tyr Tyr Ile Val Asp Lys His Gly Lys Val Thr His Glu Thr Trp           
        195                 200                 205                       

ttc gag caa ccg tat ggc gcc ttt atg cat gat ttt gct att acc cgc        672
Phe Glu Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Ile Thr Arg           
    210                 215                 220                           

aat tgg agc atc ttc ccg atc atg ccg gct acc aat tcg ttg agc cgc        720
Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Ser Arg           
225                 230                 235                 240           

ctg aaa gcg aag cag ccg att tac atg tgg gag ccg gaa ctg ggt tcc        768
Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Leu Gly Ser           
                245                 250                 255               

tat att ggt gtt ctg ccg cgt cgc ggt caa ggt agc cag atc cgc tgg        816
Tyr Ile Gly Val Leu Pro Arg Arg Gly Gln Gly Ser Gln Ile Arg Trp           
            260                 265                 270                   

ctg aaa gca cca gcg ctg tgg gtc ttt cac gtc gtc aac gcc tgg gaa        864
Leu Lys Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu           
        275                 280                 285                       

gtg ggc acc aaa atc tac att gac ctt atg gag agc gaa att ctg cca        912
Val Gly Thr Lys Ile Tyr Ile Asp Leu Met Glu Ser Glu Ile Leu Pro           
    290                 295                 300                           

ttc ccg ttc ccg aat agc caa aat cag ccg ttc gct cct gag aaa gca        960
Phe Pro Phe Pro Asn Ser Gln Asn Gln Pro Phe Ala Pro Glu Lys Ala           
305                 310                 315                 320           

gtg ccg cgt ctg acc cgt tgg gag att gat ctg gat agc agc agc gat       1008
Val Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asp Ser Ser Ser Asp           
                325                 330                 335               

gag att aag cgt acg cgt ctg cac gac ttc ttt gca gag atg ccg atc       1056
Glu Ile Lys Arg Thr Arg Leu His Asp Phe Phe Ala Glu Met Pro Ile           
            340                 345                 350                   

atg gat ttt cgt ttt gcg ctg cag tgc aac cgc tac ggt ttt atg ggt       1104
Met Asp Phe Arg Phe Ala Leu Gln Cys Asn Arg Tyr Gly Phe Met Gly           
        355                 360                 365                       

gtc gat gac ccg cgc aag ccg ctg gcg cac caa caa gcg gag aag att       1152
Val Asp Asp Pro Arg Lys Pro Leu Ala His Gln Gln Ala Glu Lys Ile           
    370                 375                 380                           

ttt gcg tac aat agc ctg ggt atc tgg gac aac cac cgt ggt gat tat       1200
Phe Ala Tyr Asn Ser Leu Gly Ile Trp Asp Asn His Arg Gly Asp Tyr           
385                 390                 395                 400           

gat ttg tgg tac agc ggc gaa gcc tca gcg gcg caa gaa ccg gct ttc       1248
Asp Leu Trp Tyr Ser Gly Glu Ala Ser Ala Ala Gln Glu Pro Ala Phe           
                405                 410                 415               

gtt ccg cgt tct ccg act gca gcg gaa ggc gac ggt tat ctg ctg acc       1296
Val Pro Arg Ser Pro Thr Ala Ala Glu Gly Asp Gly Tyr Leu Leu Thr           
            420                 425                 430                   

gtt gtg ggc cgt ttg gac gag aac cgc agc gac ctg gtc att ctg gac       1344
Val Val Gly Arg Leu Asp Glu Asn Arg Ser Asp Leu Val Ile Leu Asp           
        435                 440                 445                       

acg cag gac atc cag agc ggt ccg gtt gcg acc att aaa ctg ccg ttt       1392
Thr Gln Asp Ile Gln Ser Gly Pro Val Ala Thr Ile Lys Leu Pro Phe           
    450                 455                 460                           

cgc ctg cgt gcg gcc ctg cac ggc tgt tgg gtt ccg cgt ccg taa           1437
Arg Leu Arg Ala Ala Leu His Gly Cys Trp Val Pro Arg Pro                   
465                 470                 475                               


<210>  2
<211>  478
<212>  PRT
<213>  Isoeugenol monooxygenase of P. nitroreducens Jin1 (codon modified)

<400>  2

Met Ala Arg Leu Asn Arg Asn Asp Pro Gln Leu Val Gly Thr Leu Leu 
1               5                   10                  15      


Pro Thr Arg Ile Glu Ala Asp Leu Phe Asp Leu Glu Val Asp Gly Glu 
            20                  25                  30          


Ile Pro Lys Ser Ile Asn Gly Thr Phe Tyr Arg Asn Thr Pro Glu Pro 
        35                  40                  45              


Gln Val Thr Pro Gln Lys Phe His Thr Phe Ile Asp Gly Asp Gly Met 
    50                  55                  60                  


Ala Ser Ala Phe His Phe Glu Asp Gly His Val Asp Phe Ile Ser Arg 
65                  70                  75                  80  


Trp Val Lys Thr Ala Arg Phe Thr Ala Glu Arg Leu Ala Arg Lys Ser 
                85                  90                  95      


Leu Phe Gly Met Tyr Arg Asn Pro Tyr Thr Asp Asp Thr Ser Val Lys 
            100                 105                 110         


Gly Leu Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Ser His His Gly 
        115                 120                 125             


Lys Val Leu Ala Val Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro 
    130                 135                 140                 


Arg Thr Leu Glu Thr Arg Gly Arg Phe Asp Tyr Asp Gly Gln Val Thr 
145                 150                 155                 160 


Ser Gln Thr His Thr Ala His Pro Lys Tyr Asp Pro Glu Thr Gly Asp 
                165                 170                 175     


Leu Leu Phe Phe Gly Ser Ala Ala Lys Gly Glu Ala Thr Pro Asp Met 
            180                 185                 190         


Ala Tyr Tyr Ile Val Asp Lys His Gly Lys Val Thr His Glu Thr Trp 
        195                 200                 205             


Phe Glu Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Ile Thr Arg 
    210                 215                 220                 


Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Ser Arg 
225                 230                 235                 240 


Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Leu Gly Ser 
                245                 250                 255     


Tyr Ile Gly Val Leu Pro Arg Arg Gly Gln Gly Ser Gln Ile Arg Trp 
            260                 265                 270         


Leu Lys Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu 
        275                 280                 285             


Val Gly Thr Lys Ile Tyr Ile Asp Leu Met Glu Ser Glu Ile Leu Pro 
    290                 295                 300                 


Phe Pro Phe Pro Asn Ser Gln Asn Gln Pro Phe Ala Pro Glu Lys Ala 
305                 310                 315                 320 


Val Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asp Ser Ser Ser Asp 
                325                 330                 335     


Glu Ile Lys Arg Thr Arg Leu His Asp Phe Phe Ala Glu Met Pro Ile 
            340                 345                 350         


Met Asp Phe Arg Phe Ala Leu Gln Cys Asn Arg Tyr Gly Phe Met Gly 
        355                 360                 365             


Val Asp Asp Pro Arg Lys Pro Leu Ala His Gln Gln Ala Glu Lys Ile 
    370                 375                 380                 


Phe Ala Tyr Asn Ser Leu Gly Ile Trp Asp Asn His Arg Gly Asp Tyr 
385                 390                 395                 400 


Asp Leu Trp Tyr Ser Gly Glu Ala Ser Ala Ala Gln Glu Pro Ala Phe 
                405                 410                 415     


Val Pro Arg Ser Pro Thr Ala Ala Glu Gly Asp Gly Tyr Leu Leu Thr 
            420                 425                 430         


Val Val Gly Arg Leu Asp Glu Asn Arg Ser Asp Leu Val Ile Leu Asp 
        435                 440                 445             


Thr Gln Asp Ile Gln Ser Gly Pro Val Ala Thr Ile Lys Leu Pro Phe 
    450                 455                 460                 


Arg Leu Arg Ala Ala Leu His Gly Cys Trp Val Pro Arg Pro 
465                 470                 475             


<210>  3
<211>  1437
<212>  DNA
<213>  Isoeugenol monooxygenase of P. putida IE27


<220>
<221>  CDS
<222>  (1)..(1437)

<400>  3
atg gca acg ttt gac cgc aat gat ccg cag ttg gca gga acg atg ttc         48
Met Ala Thr Phe Asp Arg Asn Asp Pro Gln Leu Ala Gly Thr Met Phe           
1               5                   10                  15                

ccc acc cga ata gag gcg aat gtc ttt gac ctt gaa att gag ggc gag         96
Pro Thr Arg Ile Glu Ala Asn Val Phe Asp Leu Glu Ile Glu Gly Glu           
            20                  25                  30                    

atc cca cgt gca atc aac ggg agc ttc ttc cgc aac acc ccc gaa cct        144
Ile Pro Arg Ala Ile Asn Gly Ser Phe Phe Arg Asn Thr Pro Glu Pro           
        35                  40                  45                        

cag gtc acc acg caa cct ttc cac acc ttc atc gat ggg gat ggt ttg        192
Gln Val Thr Thr Gln Pro Phe His Thr Phe Ile Asp Gly Asp Gly Leu           
    50                  55                  60                            

gcg tct gct ttt cat ttc gaa gat ggc cag gtc gac ttt gtc agc cgt        240
Ala Ser Ala Phe His Phe Glu Asp Gly Gln Val Asp Phe Val Ser Arg           
65                  70                  75                  80            

tgg gta tgt act cct cgc ttt gaa gct gag cgg tcg gct cgt aaa tca        288
Trp Val Cys Thr Pro Arg Phe Glu Ala Glu Arg Ser Ala Arg Lys Ser           
                85                  90                  95                

ctc ttc ggt atg tac cgc aat ccg ttc act gat gat cca tcg gta gaa        336
Leu Phe Gly Met Tyr Arg Asn Pro Phe Thr Asp Asp Pro Ser Val Glu           
            100                 105                 110                   

ggt att gat cgt aca gtc gcc aac acc agt atc atc act cat cac ggg        384
Gly Ile Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Thr His His Gly           
        115                 120                 125                       

aaa gta ctg gcc gca aag gaa gat gga cta cct tat gag ctt gac ccc        432
Lys Val Leu Ala Ala Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro           
    130                 135                 140                           

caa acc ctg gaa acc cga ggt cgt tat gat tac aag ggg cag gta acc        480
Gln Thr Leu Glu Thr Arg Gly Arg Tyr Asp Tyr Lys Gly Gln Val Thr           
145                 150                 155                 160           

agc cat aca cat aca gcg cac cct aag ttc gac ccc cag aca ggt gaa        528
Ser His Thr His Thr Ala His Pro Lys Phe Asp Pro Gln Thr Gly Glu           
                165                 170                 175               

atg tta ctc ttc ggc tcc gct gct aaa ggc gaa cga acg ctt gat atg        576
Met Leu Leu Phe Gly Ser Ala Ala Lys Gly Glu Arg Thr Leu Asp Met           
            180                 185                 190                   

gcg tac tat att gtt gat cgc tac ggc aag gtg aca cat gag acc tgg        624
Ala Tyr Tyr Ile Val Asp Arg Tyr Gly Lys Val Thr His Glu Thr Trp           
        195                 200                 205                       

ttt aag cag cct tac ggt gca ttc atg cac gac ttt gct gtc acg cgc        672
Phe Lys Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Val Thr Arg           
    210                 215                 220                           

aac tgg tca atc ttt ccg atc atg cct gcg aca aat agc ctt gag cgt        720
Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Glu Arg           
225                 230                 235                 240           

ctt aaa gca aag cag ccc att tac atg tgg gag cct gag cga gga agc        768
Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Arg Gly Ser           
                245                 250                 255               

tat ata gga gta ctt cct cgt cgt ggt cag ggc aag gac att cgt tgg        816
Tyr Ile Gly Val Leu Pro Arg Arg Gly Gln Gly Lys Asp Ile Arg Trp           
            260                 265                 270                   

ttc cgt gcc ccg gcg ttg tgg gtt ttc cat gtc gtg aat gct tgg gag        864
Phe Arg Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu           
        275                 280                 285                       

gaa ggg aat aga att ctg att gac ttg atg gaa agt gag att ttg ccg        912
Glu Gly Asn Arg Ile Leu Ile Asp Leu Met Glu Ser Glu Ile Leu Pro           
    290                 295                 300                           

ttc cca ttc ccg aac tcg cag aac ctt cca ttt gat ccc tcc aag gct        960
Phe Pro Phe Pro Asn Ser Gln Asn Leu Pro Phe Asp Pro Ser Lys Ala           
305                 310                 315                 320           

gtt ccg cgt cta acc cgt tgg gaa att gat ctc aat agt ggt aac gat       1008
Val Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asn Ser Gly Asn Asp           
                325                 330                 335               

gag atg aaa cgt acg cag cta cac gaa tat ttt gca gaa atg cct atc       1056
Glu Met Lys Arg Thr Gln Leu His Glu Tyr Phe Ala Glu Met Pro Ile           
            340                 345                 350                   

atg gat ttc cgt ttt gcg ctc cag gat cat cgc tac gcc tac atg ggg       1104
Met Asp Phe Arg Phe Ala Leu Gln Asp His Arg Tyr Ala Tyr Met Gly           
        355                 360                 365                       

gtt gac gat cct cgt cgc ccc tta gct cat cag caa gct gaa aaa atc       1152
Val Asp Asp Pro Arg Arg Pro Leu Ala His Gln Gln Ala Glu Lys Ile           
    370                 375                 380                           

ttt gcc tac aat tcg tta ggg gtt tgg gac aac cat cgt aaa gat tat       1200
Phe Ala Tyr Asn Ser Leu Gly Val Trp Asp Asn His Arg Lys Asp Tyr           
385                 390                 395                 400           

gaa ctt tgg ttt acg gga aaa atg tct gca gcg cag gaa ccg gcg ttt       1248
Glu Leu Trp Phe Thr Gly Lys Met Ser Ala Ala Gln Glu Pro Ala Phe           
                405                 410                 415               

gtt cct aga agc cca gat gcg cct gag ggc gat ggc tac cta ctc agt       1296
Val Pro Arg Ser Pro Asp Ala Pro Glu Gly Asp Gly Tyr Leu Leu Ser           
            420                 425                 430                   

gta gta ggg cgg ctc gat gaa gat cgt agc gat cta gtt atc ctt gat       1344
Val Val Gly Arg Leu Asp Glu Asp Arg Ser Asp Leu Val Ile Leu Asp           
        435                 440                 445                       

acg caa tgt ttg gca gct ggg cct gtg gcc act gtc aag ctt ccc ttc       1392
Thr Gln Cys Leu Ala Ala Gly Pro Val Ala Thr Val Lys Leu Pro Phe           
    450                 455                 460                           

cgt ctc cga gca gcg ttg cac ggt tgt tgg cag tct aag aac tga           1437
Arg Leu Arg Ala Ala Leu His Gly Cys Trp Gln Ser Lys Asn                   
465                 470                 475                               


<210>  4
<211>  478
<212>  PRT
<213>  Isoeugenol monooxygenase of P. putida IE27

<400>  4

Met Ala Thr Phe Asp Arg Asn Asp Pro Gln Leu Ala Gly Thr Met Phe 
1               5                   10                  15      


Pro Thr Arg Ile Glu Ala Asn Val Phe Asp Leu Glu Ile Glu Gly Glu 
            20                  25                  30          


Ile Pro Arg Ala Ile Asn Gly Ser Phe Phe Arg Asn Thr Pro Glu Pro 
        35                  40                  45              


Gln Val Thr Thr Gln Pro Phe His Thr Phe Ile Asp Gly Asp Gly Leu 
    50                  55                  60                  


Ala Ser Ala Phe His Phe Glu Asp Gly Gln Val Asp Phe Val Ser Arg 
65                  70                  75                  80  


Trp Val Cys Thr Pro Arg Phe Glu Ala Glu Arg Ser Ala Arg Lys Ser 
                85                  90                  95      


Leu Phe Gly Met Tyr Arg Asn Pro Phe Thr Asp Asp Pro Ser Val Glu 
            100                 105                 110         


Gly Ile Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Thr His His Gly 
        115                 120                 125             


Lys Val Leu Ala Ala Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro 
    130                 135                 140                 


Gln Thr Leu Glu Thr Arg Gly Arg Tyr Asp Tyr Lys Gly Gln Val Thr 
145                 150                 155                 160 


Ser His Thr His Thr Ala His Pro Lys Phe Asp Pro Gln Thr Gly Glu 
                165                 170                 175     


Met Leu Leu Phe Gly Ser Ala Ala Lys Gly Glu Arg Thr Leu Asp Met 
            180                 185                 190         


Ala Tyr Tyr Ile Val Asp Arg Tyr Gly Lys Val Thr His Glu Thr Trp 
        195                 200                 205             


Phe Lys Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Val Thr Arg 
    210                 215                 220                 


Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Glu Arg 
225                 230                 235                 240 


Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Arg Gly Ser 
                245                 250                 255     


Tyr Ile Gly Val Leu Pro Arg Arg Gly Gln Gly Lys Asp Ile Arg Trp 
            260                 265                 270         


Phe Arg Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu 
        275                 280                 285             


Glu Gly Asn Arg Ile Leu Ile Asp Leu Met Glu Ser Glu Ile Leu Pro 
    290                 295                 300                 


Phe Pro Phe Pro Asn Ser Gln Asn Leu Pro Phe Asp Pro Ser Lys Ala 
305                 310                 315                 320 


Val Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asn Ser Gly Asn Asp 
                325                 330                 335     


Glu Met Lys Arg Thr Gln Leu His Glu Tyr Phe Ala Glu Met Pro Ile 
            340                 345                 350         


Met Asp Phe Arg Phe Ala Leu Gln Asp His Arg Tyr Ala Tyr Met Gly 
        355                 360                 365             


Val Asp Asp Pro Arg Arg Pro Leu Ala His Gln Gln Ala Glu Lys Ile 
    370                 375                 380                 


Phe Ala Tyr Asn Ser Leu Gly Val Trp Asp Asn His Arg Lys Asp Tyr 
385                 390                 395                 400 


Glu Leu Trp Phe Thr Gly Lys Met Ser Ala Ala Gln Glu Pro Ala Phe 
                405                 410                 415     


Val Pro Arg Ser Pro Asp Ala Pro Glu Gly Asp Gly Tyr Leu Leu Ser 
            420                 425                 430         


Val Val Gly Arg Leu Asp Glu Asp Arg Ser Asp Leu Val Ile Leu Asp 
        435                 440                 445             


Thr Gln Cys Leu Ala Ala Gly Pro Val Ala Thr Val Lys Leu Pro Phe 
    450                 455                 460                 


Arg Leu Arg Ala Ala Leu His Gly Cys Trp Gln Ser Lys Asn 
465                 470                 475             


<210>  5
<211>  1437
<212>  DNA
<213>  Isoeugenol monooxygenase of P. putida IE27 (codon modified)


<220>
<221>  CDS
<222>  (1)..(1437)

<400>  5
atg gcc act ttt gac cgc aat gac ccg caa ctg gca ggc acc atg ttc         48
Met Ala Thr Phe Asp Arg Asn Asp Pro Gln Leu Ala Gly Thr Met Phe           
1               5                   10                  15                

ccg acg cgc atc gaa gcg aat gtt ttt gat ctg gag att gaa ggt gag         96
Pro Thr Arg Ile Glu Ala Asn Val Phe Asp Leu Glu Ile Glu Gly Glu           
            20                  25                  30                    

att ccg cgt gcg atc aac ggt agc ttt ttc cgc aac acg cca gag ccg        144
Ile Pro Arg Ala Ile Asn Gly Ser Phe Phe Arg Asn Thr Pro Glu Pro           
        35                  40                  45                        

caa gtc acc acg cag ccg ttt cat act ttc atc gac ggc gac ggc ctg        192
Gln Val Thr Thr Gln Pro Phe His Thr Phe Ile Asp Gly Asp Gly Leu           
    50                  55                  60                            

gcg tca gcg ttc cac ttc gaa gat ggc cag gtc gac ttt gtg agc cgc        240
Ala Ser Ala Phe His Phe Glu Asp Gly Gln Val Asp Phe Val Ser Arg           
65                  70                  75                  80            

tgg gtc tgc acc ccg cgt ttc gag gca gag cgc agc gcg cgt aaa agc        288
Trp Val Cys Thr Pro Arg Phe Glu Ala Glu Arg Ser Ala Arg Lys Ser           
                85                  90                  95                

ctg ttt ggt atg tat cgc aat ccg ttt acg gat gac ccg agc gtt gaa        336
Leu Phe Gly Met Tyr Arg Asn Pro Phe Thr Asp Asp Pro Ser Val Glu           
            100                 105                 110                   

ggc att gac cgt acc gtg gcg aat acc tcg atc att acc cac cac ggt        384
Gly Ile Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Thr His His Gly           
        115                 120                 125                       

aag gtc ctg gca gca aaa gaa gat ggc ttg ccg tac gag tta gat ccg        432
Lys Val Leu Ala Ala Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro           
    130                 135                 140                           

cag acc ctg gaa acg cgt ggt cgc tat gac tac aag ggc cag gtt acc        480
Gln Thr Leu Glu Thr Arg Gly Arg Tyr Asp Tyr Lys Gly Gln Val Thr           
145                 150                 155                 160           

agc cat acc cac acg gct cac cct aag ttt gat ccg caa acg ggt gag        528
Ser His Thr His Thr Ala His Pro Lys Phe Asp Pro Gln Thr Gly Glu           
                165                 170                 175               

atg ctg ctg ttc ggc agc gcg gca aag ggt gag cgt acc ctg gac atg        576
Met Leu Leu Phe Gly Ser Ala Ala Lys Gly Glu Arg Thr Leu Asp Met           
            180                 185                 190                   

gcg tac tat atc gtt gac cgt tac ggt aaa gtg acc cat gaa acc tgg        624
Ala Tyr Tyr Ile Val Asp Arg Tyr Gly Lys Val Thr His Glu Thr Trp           
        195                 200                 205                       

ttc aag caa ccg tac ggc gcc ttt atg cac gac ttc gca gtc acg cgc        672
Phe Lys Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Val Thr Arg           
    210                 215                 220                           

aac tgg tct atc ttt ccg att atg ccg gcc acc aat agc ctg gag cgt        720
Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Glu Arg           
225                 230                 235                 240           

ctg aaa gct aag caa ccg att tac atg tgg gaa ccg gag cgt ggt tcc        768
Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Arg Gly Ser           
                245                 250                 255               

tac atc ggc gtg ctg ccg cgt cgt ggt cag ggt aaa gat atc cgc tgg        816
Tyr Ile Gly Val Leu Pro Arg Arg Gly Gln Gly Lys Asp Ile Arg Trp           
            260                 265                 270                   

ttc cgt gcg cct gcc ctc tgg gtg ttc cac gtt gtg aac gca tgg gaa        864
Phe Arg Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu           
        275                 280                 285                       

gag ggc aat cgt att ctg atc gat ctg atg gag agc gaa atc ctg cca        912
Glu Gly Asn Arg Ile Leu Ile Asp Leu Met Glu Ser Glu Ile Leu Pro           
    290                 295                 300                           

ttc ccg ttt ccg aac tct cag aat ctg ccg ttc gat ccg agc aaa gcc        960
Phe Pro Phe Pro Asn Ser Gln Asn Leu Pro Phe Asp Pro Ser Lys Ala           
305                 310                 315                 320           

gta ccg cgc ttg acc cgt tgg gag att gat ttg aac agc ggt aat gac       1008
Val Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asn Ser Gly Asn Asp           
                325                 330                 335               

gag atg aag cgt act cag ctg cac gaa tac ttc gct gag atg ccg att       1056
Glu Met Lys Arg Thr Gln Leu His Glu Tyr Phe Ala Glu Met Pro Ile           
            340                 345                 350                   

atg gac ttt cgt ttc gcg ctg caa gat cac cgt tac gcg tat atg ggt       1104
Met Asp Phe Arg Phe Ala Leu Gln Asp His Arg Tyr Ala Tyr Met Gly           
        355                 360                 365                       

gtt gat gat cca cgc cgt cca ttg gcg cat caa caa gcg gaa aag att       1152
Val Asp Asp Pro Arg Arg Pro Leu Ala His Gln Gln Ala Glu Lys Ile           
    370                 375                 380                           

ttt gcg tat aac agc ctg ggt gtt tgg gac aac cat cgt aaa gac tat       1200
Phe Ala Tyr Asn Ser Leu Gly Val Trp Asp Asn His Arg Lys Asp Tyr           
385                 390                 395                 400           

gag ctg tgg ttt acg ggt aaa atg tcc gcg gct cag gaa ccg gcc ttc       1248
Glu Leu Trp Phe Thr Gly Lys Met Ser Ala Ala Gln Glu Pro Ala Phe           
                405                 410                 415               

gtg ccg cgc agc ccg gac gcc cct gag ggt gat ggt tat ttg ctg tcc       1296
Val Pro Arg Ser Pro Asp Ala Pro Glu Gly Asp Gly Tyr Leu Leu Ser           
            420                 425                 430                   

gtc gtg ggt cgc ctg gat gaa gat cgt agc gac ctg gtt atc ctg gac       1344
Val Val Gly Arg Leu Asp Glu Asp Arg Ser Asp Leu Val Ile Leu Asp           
        435                 440                 445                       

acc cag tgc ctt gcg gca ggc ccg gtt gcg acc gtc aag ctg ccg ttc       1392
Thr Gln Cys Leu Ala Ala Gly Pro Val Ala Thr Val Lys Leu Pro Phe           
    450                 455                 460                           

cgt ctg cgt gca gct ctg cat ggt tgt tgg cag agc aaa aac taa           1437
Arg Leu Arg Ala Ala Leu His Gly Cys Trp Gln Ser Lys Asn                   
465                 470                 475                               


<210>  6
<211>  478
<212>  PRT
<213>  Isoeugenol monooxygenase of P. putida IE27 (codon modified)

<400>  6

Met Ala Thr Phe Asp Arg Asn Asp Pro Gln Leu Ala Gly Thr Met Phe 
1               5                   10                  15      


Pro Thr Arg Ile Glu Ala Asn Val Phe Asp Leu Glu Ile Glu Gly Glu 
            20                  25                  30          


Ile Pro Arg Ala Ile Asn Gly Ser Phe Phe Arg Asn Thr Pro Glu Pro 
        35                  40                  45              


Gln Val Thr Thr Gln Pro Phe His Thr Phe Ile Asp Gly Asp Gly Leu 
    50                  55                  60                  


Ala Ser Ala Phe His Phe Glu Asp Gly Gln Val Asp Phe Val Ser Arg 
65                  70                  75                  80  


Trp Val Cys Thr Pro Arg Phe Glu Ala Glu Arg Ser Ala Arg Lys Ser 
                85                  90                  95      


Leu Phe Gly Met Tyr Arg Asn Pro Phe Thr Asp Asp Pro Ser Val Glu 
            100                 105                 110         


Gly Ile Asp Arg Thr Val Ala Asn Thr Ser Ile Ile Thr His His Gly 
        115                 120                 125             


Lys Val Leu Ala Ala Lys Glu Asp Gly Leu Pro Tyr Glu Leu Asp Pro 
    130                 135                 140                 


Gln Thr Leu Glu Thr Arg Gly Arg Tyr Asp Tyr Lys Gly Gln Val Thr 
145                 150                 155                 160 


Ser His Thr His Thr Ala His Pro Lys Phe Asp Pro Gln Thr Gly Glu 
                165                 170                 175     


Met Leu Leu Phe Gly Ser Ala Ala Lys Gly Glu Arg Thr Leu Asp Met 
            180                 185                 190         


Ala Tyr Tyr Ile Val Asp Arg Tyr Gly Lys Val Thr His Glu Thr Trp 
        195                 200                 205             


Phe Lys Gln Pro Tyr Gly Ala Phe Met His Asp Phe Ala Val Thr Arg 
    210                 215                 220                 


Asn Trp Ser Ile Phe Pro Ile Met Pro Ala Thr Asn Ser Leu Glu Arg 
225                 230                 235                 240 


Leu Lys Ala Lys Gln Pro Ile Tyr Met Trp Glu Pro Glu Arg Gly Ser 
                245                 250                 255     


Tyr Ile Gly Val Leu Pro Arg Arg Gly Gln Gly Lys Asp Ile Arg Trp 
            260                 265                 270         


Phe Arg Ala Pro Ala Leu Trp Val Phe His Val Val Asn Ala Trp Glu 
        275                 280                 285             


Glu Gly Asn Arg Ile Leu Ile Asp Leu Met Glu Ser Glu Ile Leu Pro 
    290                 295                 300                 


Phe Pro Phe Pro Asn Ser Gln Asn Leu Pro Phe Asp Pro Ser Lys Ala 
305                 310                 315                 320 


Val Pro Arg Leu Thr Arg Trp Glu Ile Asp Leu Asn Ser Gly Asn Asp 
                325                 330                 335     


Glu Met Lys Arg Thr Gln Leu His Glu Tyr Phe Ala Glu Met Pro Ile 
            340                 345                 350         


Met Asp Phe Arg Phe Ala Leu Gln Asp His Arg Tyr Ala Tyr Met Gly 
        355                 360                 365             


Val Asp Asp Pro Arg Arg Pro Leu Ala His Gln Gln Ala Glu Lys Ile 
    370                 375                 380                 


Phe Ala Tyr Asn Ser Leu Gly Val Trp Asp Asn His Arg Lys Asp Tyr 
385                 390                 395                 400 


Glu Leu Trp Phe Thr Gly Lys Met Ser Ala Ala Gln Glu Pro Ala Phe 
                405                 410                 415     


Val Pro Arg Ser Pro Asp Ala Pro Glu Gly Asp Gly Tyr Leu Leu Ser 
            420                 425                 430         


Val Val Gly Arg Leu Asp Glu Asp Arg Ser Asp Leu Val Ile Leu Asp 
        435                 440                 445             


Thr Gln Cys Leu Ala Ala Gly Pro Val Ala Thr Val Lys Leu Pro Phe 
    450                 455                 460                 


Arg Leu Arg Ala Ala Leu His Gly Cys Trp Gln Ser Lys Asn 
465                 470                 475             


<210>  7
<211>  1647
<212>  DNA
<213>  Chaperonin GroEL of E. coli


<220>
<221>  CDS
<222>  (1)..(1647)

<400>  7
atg gca gct aaa gac gta aaa ttc ggt aac gac gct cgt gtg aaa atg         48
Met Ala Ala Lys Asp Val Lys Phe Gly Asn Asp Ala Arg Val Lys Met           
1               5                   10                  15                

ctg cgc ggc gta aac gta ctg gca gat gca gtg aaa gtt acc ctc ggt         96
Leu Arg Gly Val Asn Val Leu Ala Asp Ala Val Lys Val Thr Leu Gly           
            20                  25                  30                    

cca aaa ggc cgt aac gta gtt ctg gat aaa tct ttc ggt gca ccg acc        144
Pro Lys Gly Arg Asn Val Val Leu Asp Lys Ser Phe Gly Ala Pro Thr           
        35                  40                  45                        

atc acc aaa gat ggt gtt tcc gtt gct cgt gaa atc gaa ctg gaa gac        192
Ile Thr Lys Asp Gly Val Ser Val Ala Arg Glu Ile Glu Leu Glu Asp           
    50                  55                  60                            

aag ttc gaa aat atg ggt gcg cag atg gtg aaa gaa gtt gcc tct aaa        240
Lys Phe Glu Asn Met Gly Ala Gln Met Val Lys Glu Val Ala Ser Lys           
65                  70                  75                  80            

gca aac gac gct gca ggc gac ggt acc acc act gca acc gta ctg gct        288
Ala Asn Asp Ala Ala Gly Asp Gly Thr Thr Thr Ala Thr Val Leu Ala           
                85                  90                  95                

cag gct atc atc act gaa ggt ctg aaa gct gtt gct gcg ggc atg aac        336
Gln Ala Ile Ile Thr Glu Gly Leu Lys Ala Val Ala Ala Gly Met Asn           
            100                 105                 110                   

ccg atg gac ctg aaa cgt ggt atc gac aaa gcg gtt acc gct gca gtt        384
Pro Met Asp Leu Lys Arg Gly Ile Asp Lys Ala Val Thr Ala Ala Val           
        115                 120                 125                       

gaa gaa ctg aaa gcg ctg tcc gta cca tgc tct gac tct aaa gcg att        432
Glu Glu Leu Lys Ala Leu Ser Val Pro Cys Ser Asp Ser Lys Ala Ile           
    130                 135                 140                           

gct cag gtt ggt acc atc tcc gct aac tcc gac gaa acc gta ggt aaa        480
Ala Gln Val Gly Thr Ile Ser Ala Asn Ser Asp Glu Thr Val Gly Lys           
145                 150                 155                 160           

ctg atc gct gaa gcg atg gac aaa gtc ggt aaa gaa ggc gtt atc acc        528
Leu Ile Ala Glu Ala Met Asp Lys Val Gly Lys Glu Gly Val Ile Thr           
                165                 170                 175               

gtt gaa gac ggt acc ggt ctg cag gac gaa ctg gac gtg gtt gaa ggt        576
Val Glu Asp Gly Thr Gly Leu Gln Asp Glu Leu Asp Val Val Glu Gly           
            180                 185                 190                   

atg cag ttc gac cgt ggc tac ctg tct cct tac ttc atc aac aag ccg        624
Met Gln Phe Asp Arg Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Lys Pro           
        195                 200                 205                       

gaa act ggc gca gta gaa ctg gaa agc ccg ttc atc ctg ctg gct gac        672
Glu Thr Gly Ala Val Glu Leu Glu Ser Pro Phe Ile Leu Leu Ala Asp           
    210                 215                 220                           

aag aaa atc tcc aac atc cgc gaa atg ctg ccg gtt ctg gaa gct gtt        720
Lys Lys Ile Ser Asn Ile Arg Glu Met Leu Pro Val Leu Glu Ala Val           
225                 230                 235                 240           

gcc aaa gca ggc aaa ccg ctg ctg atc atc gct gaa gat gta gaa ggc        768
Ala Lys Ala Gly Lys Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly           
                245                 250                 255               

gaa gcg ctg gca act ctg gtt gtt aac acc atg cgt ggc atc gtg aaa        816
Glu Ala Leu Ala Thr Leu Val Val Asn Thr Met Arg Gly Ile Val Lys           
            260                 265                 270                   

gtc gct gcg gtt aaa gca ccg ggc ttc ggc gat cgt cgt aaa gct atg        864
Val Ala Ala Val Lys Ala Pro Gly Phe Gly Asp Arg Arg Lys Ala Met           
        275                 280                 285                       

ctg cag gat atc gca acc ctg act ggc ggt acc gtg atc tct gaa gag        912
Leu Gln Asp Ile Ala Thr Leu Thr Gly Gly Thr Val Ile Ser Glu Glu           
    290                 295                 300                           

atc ggt atg gag ctg gaa aaa gca acc ctg gaa gac ctg ggt cag gct        960
Ile Gly Met Glu Leu Glu Lys Ala Thr Leu Glu Asp Leu Gly Gln Ala           
305                 310                 315                 320           

aaa cgt gtt gtg atc aac aaa gac acc acc act atc atc gat ggc gtg       1008
Lys Arg Val Val Ile Asn Lys Asp Thr Thr Thr Ile Ile Asp Gly Val           
                325                 330                 335               

ggt gaa gaa gct gca atc cag ggc cgt gtt gct cag atc cgt cag cag       1056
Gly Glu Glu Ala Ala Ile Gln Gly Arg Val Ala Gln Ile Arg Gln Gln           
            340                 345                 350                   

att gaa gaa gca act tct gac tac gac cgt gaa aaa ctg cag gaa cgc       1104
Ile Glu Glu Ala Thr Ser Asp Tyr Asp Arg Glu Lys Leu Gln Glu Arg           
        355                 360                 365                       

gta gcg aaa ctg gca ggc ggc gtt gca gtt atc aaa gtg ggt gct gct       1152
Val Ala Lys Leu Ala Gly Gly Val Ala Val Ile Lys Val Gly Ala Ala           
    370                 375                 380                           

acc gaa gtt gaa atg aaa gag aaa aaa gca cgc gtt gaa gat gcc ctg       1200
Thr Glu Val Glu Met Lys Glu Lys Lys Ala Arg Val Glu Asp Ala Leu           
385                 390                 395                 400           

cac gcg acc cgt gct gcg gta gaa gaa ggc gtg gtt gct ggt ggt ggt       1248
His Ala Thr Arg Ala Ala Val Glu Glu Gly Val Val Ala Gly Gly Gly           
                405                 410                 415               

gtt gcg ctg atc cgc gta gcg tct aaa ctg gct gac ctg cgt ggt cag       1296
Val Ala Leu Ile Arg Val Ala Ser Lys Leu Ala Asp Leu Arg Gly Gln           
            420                 425                 430                   

aac gaa gac cag aac gtg ggt atc aaa gtt gca ctg cgt gca atg gaa       1344
Asn Glu Asp Gln Asn Val Gly Ile Lys Val Ala Leu Arg Ala Met Glu           
        435                 440                 445                       

gct ccg ctg cgt cag atc gta ttg aac tgc ggc gaa gaa ccg tct gtt       1392
Ala Pro Leu Arg Gln Ile Val Leu Asn Cys Gly Glu Glu Pro Ser Val           
    450                 455                 460                           

gtt gct aac acc gtt aaa ggc ggc gac ggc aac tac ggt tac aac gca       1440
Val Ala Asn Thr Val Lys Gly Gly Asp Gly Asn Tyr Gly Tyr Asn Ala           
465                 470                 475                 480           

gca acc gaa gaa tac ggc aac atg atc gac atg ggt atc ctg gat cca       1488
Ala Thr Glu Glu Tyr Gly Asn Met Ile Asp Met Gly Ile Leu Asp Pro           
                485                 490                 495               

acc aaa gta act cgt tct gct ctg cag tac gca gct tct gtg gct ggc       1536
Thr Lys Val Thr Arg Ser Ala Leu Gln Tyr Ala Ala Ser Val Ala Gly           
            500                 505                 510                   

ctg atg atc acc acc gaa tgc atg gtt acc gac ctg ccg aaa aac gat       1584
Leu Met Ile Thr Thr Glu Cys Met Val Thr Asp Leu Pro Lys Asn Asp           
        515                 520                 525                       

gca gct gac tta ggc gct gct ggc ggt atg ggc ggc atg ggt ggc atg       1632
Ala Ala Asp Leu Gly Ala Ala Gly Gly Met Gly Gly Met Gly Gly Met           
    530                 535                 540                           

ggc ggc atg atg taa                                                   1647
Gly Gly Met Met                                                           
545                                                                       


<210>  8
<211>  548
<212>  PRT
<213>  Chaperonin GroEL of E. coli

<400>  8

Met Ala Ala Lys Asp Val Lys Phe Gly Asn Asp Ala Arg Val Lys Met 
1               5                   10                  15      


Leu Arg Gly Val Asn Val Leu Ala Asp Ala Val Lys Val Thr Leu Gly 
            20                  25                  30          


Pro Lys Gly Arg Asn Val Val Leu Asp Lys Ser Phe Gly Ala Pro Thr 
        35                  40                  45              


Ile Thr Lys Asp Gly Val Ser Val Ala Arg Glu Ile Glu Leu Glu Asp 
    50                  55                  60                  


Lys Phe Glu Asn Met Gly Ala Gln Met Val Lys Glu Val Ala Ser Lys 
65                  70                  75                  80  


Ala Asn Asp Ala Ala Gly Asp Gly Thr Thr Thr Ala Thr Val Leu Ala 
                85                  90                  95      


Gln Ala Ile Ile Thr Glu Gly Leu Lys Ala Val Ala Ala Gly Met Asn 
            100                 105                 110         


Pro Met Asp Leu Lys Arg Gly Ile Asp Lys Ala Val Thr Ala Ala Val 
        115                 120                 125             


Glu Glu Leu Lys Ala Leu Ser Val Pro Cys Ser Asp Ser Lys Ala Ile 
    130                 135                 140                 


Ala Gln Val Gly Thr Ile Ser Ala Asn Ser Asp Glu Thr Val Gly Lys 
145                 150                 155                 160 


Leu Ile Ala Glu Ala Met Asp Lys Val Gly Lys Glu Gly Val Ile Thr 
                165                 170                 175     


Val Glu Asp Gly Thr Gly Leu Gln Asp Glu Leu Asp Val Val Glu Gly 
            180                 185                 190         


Met Gln Phe Asp Arg Gly Tyr Leu Ser Pro Tyr Phe Ile Asn Lys Pro 
        195                 200                 205             


Glu Thr Gly Ala Val Glu Leu Glu Ser Pro Phe Ile Leu Leu Ala Asp 
    210                 215                 220                 


Lys Lys Ile Ser Asn Ile Arg Glu Met Leu Pro Val Leu Glu Ala Val 
225                 230                 235                 240 


Ala Lys Ala Gly Lys Pro Leu Leu Ile Ile Ala Glu Asp Val Glu Gly 
                245                 250                 255     


Glu Ala Leu Ala Thr Leu Val Val Asn Thr Met Arg Gly Ile Val Lys 
            260                 265                 270         


Val Ala Ala Val Lys Ala Pro Gly Phe Gly Asp Arg Arg Lys Ala Met 
        275                 280                 285             


Leu Gln Asp Ile Ala Thr Leu Thr Gly Gly Thr Val Ile Ser Glu Glu 
    290                 295                 300                 


Ile Gly Met Glu Leu Glu Lys Ala Thr Leu Glu Asp Leu Gly Gln Ala 
305                 310                 315                 320 


Lys Arg Val Val Ile Asn Lys Asp Thr Thr Thr Ile Ile Asp Gly Val 
                325                 330                 335     


Gly Glu Glu Ala Ala Ile Gln Gly Arg Val Ala Gln Ile Arg Gln Gln 
            340                 345                 350         


Ile Glu Glu Ala Thr Ser Asp Tyr Asp Arg Glu Lys Leu Gln Glu Arg 
        355                 360                 365             


Val Ala Lys Leu Ala Gly Gly Val Ala Val Ile Lys Val Gly Ala Ala 
    370                 375                 380                 


Thr Glu Val Glu Met Lys Glu Lys Lys Ala Arg Val Glu Asp Ala Leu 
385                 390                 395                 400 


His Ala Thr Arg Ala Ala Val Glu Glu Gly Val Val Ala Gly Gly Gly 
                405                 410                 415     


Val Ala Leu Ile Arg Val Ala Ser Lys Leu Ala Asp Leu Arg Gly Gln 
            420                 425                 430         


Asn Glu Asp Gln Asn Val Gly Ile Lys Val Ala Leu Arg Ala Met Glu 
        435                 440                 445             


Ala Pro Leu Arg Gln Ile Val Leu Asn Cys Gly Glu Glu Pro Ser Val 
    450                 455                 460                 


Val Ala Asn Thr Val Lys Gly Gly Asp Gly Asn Tyr Gly Tyr Asn Ala 
465                 470                 475                 480 


Ala Thr Glu Glu Tyr Gly Asn Met Ile Asp Met Gly Ile Leu Asp Pro 
                485                 490                 495     


Thr Lys Val Thr Arg Ser Ala Leu Gln Tyr Ala Ala Ser Val Ala Gly 
            500                 505                 510         


Leu Met Ile Thr Thr Glu Cys Met Val Thr Asp Leu Pro Lys Asn Asp 
        515                 520                 525             


Ala Ala Asp Leu Gly Ala Ala Gly Gly Met Gly Gly Met Gly Gly Met 
    530                 535                 540                 


Gly Gly Met Met 
545             


<210>  9
<211>  294
<212>  DNA
<213>  Chaperonin GroES of E.coli


<220>
<221>  CDS
<222>  (1)..(294)

<400>  9
atg aat att cgt cca ttg cat gat cgc gtg atc gtc aag cgt aaa gaa         48
Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu           
1               5                   10                  15                

gtt gaa act aaa tct gct ggc ggc atc gtt ctg acc ggc tct gca gcg         96
Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala           
            20                  25                  30                    

gct aaa tcc acc cgc ggc gaa gtg ctg gct gtc ggc aat ggc cgt atc        144
Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile           
        35                  40                  45                        

ctt gaa aat ggc gaa gtg aag ccg ctg gat gtg aaa gtt ggc gac atc        192
Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile           
    50                  55                  60                            

gtt att ttc aac gat ggc tac ggt gtg aaa tct gag aag atc gac aat        240
Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn           
65                  70                  75                  80            

gaa gaa gtg ttg atc atg tcc gaa agc gac att ctg gca att gtt gaa        288
Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu           
                85                  90                  95                

gcg taa                                                                294
Ala                                                                       
                                                                          


<210>  10
<211>  97
<212>  PRT
<213>  Chaperonin GroES of E.coli

<400>  10

Met Asn Ile Arg Pro Leu His Asp Arg Val Ile Val Lys Arg Lys Glu 
1               5                   10                  15      


Val Glu Thr Lys Ser Ala Gly Gly Ile Val Leu Thr Gly Ser Ala Ala 
            20                  25                  30          


Ala Lys Ser Thr Arg Gly Glu Val Leu Ala Val Gly Asn Gly Arg Ile 
        35                  40                  45              


Leu Glu Asn Gly Glu Val Lys Pro Leu Asp Val Lys Val Gly Asp Ile 
    50                  55                  60                  


Val Ile Phe Asn Asp Gly Tyr Gly Val Lys Ser Glu Lys Ile Asp Asn 
65                  70                  75                  80  


Glu Glu Val Leu Ile Met Ser Glu Ser Asp Ile Leu Ala Ile Val Glu 
                85                  90                  95      


Ala 
    


<210>  11
<211>  3451
<212>  DNA
<213>  PC1 (polycistroncic construct containing codon modified isoeugenol monooxygenase of P. nitroreducens Jin 1, groES, groEL)

<400>  11
gaaggagata tacatatggc acgtctgaac cgtaatgatc cgcaactcgt cggcaccctg       60

ttaccaaccc gtattgaggc tgacctgttc gacctggaag tggacggcga aattccgaag      120

tccatcaacg gtaccttcta ccgtaacacg ccggagcctc aggtgacgcc gcagaaattc      180

cacaccttca tcgatggtga cggcatggcg tctgcatttc attttgaaga tggccacgtg      240

gacttcatca gccgctgggt taaaaccgcg cgtttcacgg cggagagact ggcacgtaaa      300

agcctgttcg gtatgtaccg taatccgtac accgatgaca cgtctgtgaa gggtctggat      360

cgtaccgttg ccaacacgag catcatcagc catcacggta aggttctggc ggtgaaagaa      420

gatggcttgc cgtacgagct ggacccacgc actctggaaa cccgtggtcg ctttgactat      480

gatggccaag tcaccagcca gacgcacacc gcgcatccga agtatgaccc ggaaaccggt      540

gacctgttgt tctttggctc cgcagccaag ggtgaggcaa cgcctgatat ggcctattac      600

atcgttgata aacatggtaa ggtaacgcat gagacttggt tcgagcaacc gtatggcgcc      660

tttatgcatg attttgctat tacccgcaat tggagcatct tcccgatcat gccggctacc      720

aattcgttga gccgcctgaa agcgaagcag ccgatttaca tgtgggagcc ggaactgggt      780

tcctatattg gtgttctgcc gcgtcgcggt caaggtagcc agatccgctg gctgaaagca      840

ccagcgctgt gggtctttca cgtcgtcaac gcctgggaag tgggcaccaa aatctacatt      900

gaccttatgg agagcgaaat tctgccattc ccgttcccga atagccaaaa tcagccgttc      960

gctcctgaga aagcagtgcc gcgtctgacc cgttgggaga ttgatctgga tagcagcagc     1020

gatgagatta agcgtacgcg tctgcacgac ttctttgcag agatgccgat catggatttt     1080

cgttttgcgc tgcagtgcaa ccgctacggt tttatgggtg tcgatgaccc gcgcaagccg     1140

ctggcgcacc aacaagcgga gaagattttt gcgtacaata gcctgggtat ctgggacaac     1200

caccgtggtg attatgattt gtggtacagc ggcgaagcct cagcggcgca agaaccggct     1260

ttcgttccgc gttctccgac tgcagcggaa ggcgacggtt atctgctgac cgttgtgggc     1320

cgtttggacg agaaccgcag cgacctggtc attctggaca cgcaggacat ccagagcggt     1380

ccggttgcga ccattaaact gccgtttcgc ctgcgtgcgg ccctgcacgg ctgttgggtt     1440

ccgcgtccgt aagaaggaga tatacatatg aatattcgtc cattgcatga tcgcgtgatc     1500

gtcaagcgta aagaagttga aactaaatct gctggcggca tcgttctgac cggctctgca     1560

gcggctaaat ccacccgcgg cgaagtgctg gctgtcggca atggccgtat ccttgaaaat     1620

ggcgaagtga agccgctgga tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac     1680

ggtgtgaaat ctgagaagat cgacaatgaa gaagtgttga tcatgtccga aagcgacatt     1740

ctggcaattg ttgaagcgta atccgcgcac gacactgaac atacgaattt aaggaataaa     1800

gataatggca gctaaagacg taaaattcgg taacgacgct cgtgtgaaaa tgctgcgcgg     1860

cgtaaacgta ctggcagatg cagtgaaagt taccctcggt ccaaaaggcc gtaacgtagt     1920

tctggataaa tctttcggtg caccgaccat caccaaagat ggtgtttccg ttgctcgtga     1980

aatcgaactg gaagacaagt tcgaaaatat gggtgcgcag atggtgaaag aagttgcctc     2040

taaagcaaac gacgctgcag gcgacggtac caccactgca accgtactgg ctcaggctat     2100

catcactgaa ggtctgaaag ctgttgctgc gggcatgaac ccgatggacc tgaaacgtgg     2160

tatcgacaaa gcggttaccg ctgcagttga agaactgaaa gcgctgtccg taccatgctc     2220

tgactctaaa gcgattgctc aggttggtac catctccgct aactccgacg aaaccgtagg     2280

taaactgatc gctgaagcga tggacaaagt cggtaaagaa ggcgttatca ccgttgaaga     2340

cggtaccggt ctgcaggacg aactggacgt ggttgaaggt atgcagttcg accgtggcta     2400

cctgtctcct tacttcatca acaagccgga aactggcgca gtagaactgg aaagcccgtt     2460

catcctgctg gctgacaaga aaatctccaa catccgcgaa atgctgccgg ttctggaagc     2520

tgttgccaaa gcaggcaaac cgctgctgat catcgctgaa gatgtagaag gcgaagcgct     2580

ggcaactctg gttgttaaca ccatgcgtgg catcgtgaaa gtcgctgcgg ttaaagcacc     2640

gggcttcggc gatcgtcgta aagctatgct gcaggatatc gcaaccctga ctggcggtac     2700

cgtgatctct gaagagatcg gtatggagct ggaaaaagca accctggaag acctgggtca     2760

ggctaaacgt gttgtgatca acaaagacac caccactatc atcgatggcg tgggtgaaga     2820

agctgcaatc cagggccgtg ttgctcagat ccgtcagcag attgaagaag caacttctga     2880

ctacgaccgt gaaaaactgc aggaacgcgt agcgaaactg gcaggcggcg ttgcagttat     2940

caaagtgggt gctgctaccg aagttgaaat gaaagagaaa aaagcacgcg ttgaagatgc     3000

cctgcacgcg acccgtgctg cggtagaaga aggcgtggtt gctggtggtg gtgttgcgct     3060

gatccgcgta gcgtctaaac tggctgacct gcgtggtcag aacgaagacc agaacgtggg     3120

tatcaaagtt gcactgcgtg caatggaagc tccgctgcgt cagatcgtat tgaactgcgg     3180

cgaagaaccg tctgttgttg ctaacaccgt taaaggcggc gacggcaact acggttacaa     3240

cgcagcaacc gaagaatacg gcaacatgat cgacatgggt atcctggatc caaccaaagt     3300

aactcgttct gctctgcagt acgcagcttc tgtggctggc ctgatgatca ccaccgaatg     3360

catggttacc gacctgccga aaaacgatgc agctgactta ggcgctgctg gcggtatggg     3420

cggcatgggt ggcatgggcg gcatgatgta a                                    3451


<210>  12
<211>  3475
<212>  DNA
<213>  PC2 (polycistroncic construct containing codon modified isoeugenol monooxygenase of P. nitroreducens Jin 1, groES, groEL)

<400>  12
gaaggagata tacatatggc acgtctgaac cgtaatgatc cgcaactcgt cggcaccctg       60

ttaccaaccc gtattgaggc tgacctgttc gacctggaag tggacggcga aattccgaag      120

tccatcaacg gtaccttcta ccgtaacacg ccggagcctc aggtgacgcc gcagaaattc      180

cacaccttca tcgatggtga cggcatggcg tctgcatttc attttgaaga tggccacgtg      240

gacttcatca gccgctgggt taaaaccgcg cgtttcacgg cggagagact ggcacgtaaa      300

agcctgttcg gtatgtaccg taatccgtac accgatgaca cgtctgtgaa gggtctggat      360

cgtaccgttg ccaacacgag catcatcagc catcacggta aggttctggc ggtgaaagaa      420

gatggcttgc cgtacgagct ggacccacgc actctggaaa cccgtggtcg ctttgactat      480

gatggccaag tcaccagcca gacgcacacc gcgcatccga agtatgaccc ggaaaccggt      540

gacctgttgt tctttggctc cgcagccaag ggtgaggcaa cgcctgatat ggcctattac      600

atcgttgata aacatggtaa ggtaacgcat gagacttggt tcgagcaacc gtatggcgcc      660

tttatgcatg attttgctat tacccgcaat tggagcatct tcccgatcat gccggctacc      720

aattcgttga gccgcctgaa agcgaagcag ccgatttaca tgtgggagcc ggaactgggt      780

tcctatattg gtgttctgcc gcgtcgcggt caaggtagcc agatccgctg gctgaaagca      840

ccagcgctgt gggtctttca cgtcgtcaac gcctgggaag tgggcaccaa aatctacatt      900

gaccttatgg agagcgaaat tctgccattc ccgttcccga atagccaaaa tcagccgttc      960

gctcctgaga aagcagtgcc gcgtctgacc cgttgggaga ttgatctgga tagcagcagc     1020

gatgagatta agcgtacgcg tctgcacgac ttctttgcag agatgccgat catggatttt     1080

cgttttgcgc tgcagtgcaa ccgctacggt tttatgggtg tcgatgaccc gcgcaagccg     1140

ctggcgcacc aacaagcgga gaagattttt gcgtacaata gcctgggtat ctgggacaac     1200

caccgtggtg attatgattt gtggtacagc ggcgaagcct cagcggcgca agaaccggct     1260

ttcgttccgc gttctccgac tgcagcggaa ggcgacggtt atctgctgac cgttgtgggc     1320

cgtttggacg agaaccgcag cgacctggtc attctggaca cgcaggacat ccagagcggt     1380

ccggttgcga ccattaaact gccgtttcgc ctgcgtgcgg ccctgcacgg ctgttgggtt     1440

ccgcgtccgt aatccgcgca cgacactgaa catacggaag gagatataca tatgaatatt     1500

cgtccattgc atgatcgcgt gatcgtcaag cgtaaagaag ttgaaactaa atctgctggc     1560

ggcatcgttc tgaccggctc tgcagcggct aaatccaccc gcggcgaagt gctggctgtc     1620

ggcaatggcc gtatccttga aaatggcgaa gtgaagccgc tggatgtgaa agttggcgac     1680

atcgttattt tcaacgatgg ctacggtgtg aaatctgaga agatcgacaa tgaagaagtg     1740

ttgatcatgt ccgaaagcga cattctggca attgttgaag cgtaatccgc gcacgacact     1800

gaacatacga atttaaggaa taaagataat ggcagctaaa gacgtaaaat tcggtaacga     1860

cgctcgtgtg aaaatgctgc gcggcgtaaa cgtactggca gatgcagtga aagttaccct     1920

cggtccaaaa ggccgtaacg tagttctgga taaatctttc ggtgcaccga ccatcaccaa     1980

agatggtgtt tccgttgctc gtgaaatcga actggaagac aagttcgaaa atatgggtgc     2040

gcagatggtg aaagaagttg cctctaaagc aaacgacgct gcaggcgacg gtaccaccac     2100

tgcaaccgta ctggctcagg ctatcatcac tgaaggtctg aaagctgttg ctgcgggcat     2160

gaacccgatg gacctgaaac gtggtatcga caaagcggtt accgctgcag ttgaagaact     2220

gaaagcgctg tccgtaccat gctctgactc taaagcgatt gctcaggttg gtaccatctc     2280

cgctaactcc gacgaaaccg taggtaaact gatcgctgaa gcgatggaca aagtcggtaa     2340

agaaggcgtt atcaccgttg aagacggtac cggtctgcag gacgaactgg acgtggttga     2400

aggtatgcag ttcgaccgtg gctacctgtc tccttacttc atcaacaagc cggaaactgg     2460

cgcagtagaa ctggaaagcc cgttcatcct gctggctgac aagaaaatct ccaacatccg     2520

cgaaatgctg ccggttctgg aagctgttgc caaagcaggc aaaccgctgc tgatcatcgc     2580

tgaagatgta gaaggcgaag cgctggcaac tctggttgtt aacaccatgc gtggcatcgt     2640

gaaagtcgct gcggttaaag caccgggctt cggcgatcgt cgtaaagcta tgctgcagga     2700

tatcgcaacc ctgactggcg gtaccgtgat ctctgaagag atcggtatgg agctggaaaa     2760

agcaaccctg gaagacctgg gtcaggctaa acgtgttgtg atcaacaaag acaccaccac     2820

tatcatcgat ggcgtgggtg aagaagctgc aatccagggc cgtgttgctc agatccgtca     2880

gcagattgaa gaagcaactt ctgactacga ccgtgaaaaa ctgcaggaac gcgtagcgaa     2940

actggcaggc ggcgttgcag ttatcaaagt gggtgctgct accgaagttg aaatgaaaga     3000

gaaaaaagca cgcgttgaag atgccctgca cgcgacccgt gctgcggtag aagaaggcgt     3060

ggttgctggt ggtggtgttg cgctgatccg cgtagcgtct aaactggctg acctgcgtgg     3120

tcagaacgaa gaccagaacg tgggtatcaa agttgcactg cgtgcaatgg aagctccgct     3180

gcgtcagatc gtattgaact gcggcgaaga accgtctgtt gttgctaaca ccgttaaagg     3240

cggcgacggc aactacggtt acaacgcagc aaccgaagaa tacggcaaca tgatcgacat     3300

gggtatcctg gatccaacca aagtaactcg ttctgctctg cagtacgcag cttctgtggc     3360

tggcctgatg atcaccaccg aatgcatggt taccgacctg ccgaaaaacg atgcagctga     3420

cttaggcgct gctggcggta tgggcggcat gggtggcatg ggcggcatga tgtaa          3475


<210>  13
<211>  3456
<212>  DNA
<213>  PC3 (polycistroncic construct containing codon modified isoeugenol monooxygenase of P. nitroreducens Jin 1, groES, groEL)

<400>  13
gaaggagata tacatatggc acgtctgaac cgtaatgatc cgcaactcgt cggcaccctg       60

ttaccaaccc gtattgaggc tgacctgttc gacctggaag tggacggcga aattccgaag      120

tccatcaacg gtaccttcta ccgtaacacg ccggagcctc aggtgacgcc gcagaaattc      180

cacaccttca tcgatggtga cggcatggcg tctgcatttc attttgaaga tggccacgtg      240

gacttcatca gccgctgggt taaaaccgcg cgtttcacgg cggagagact ggcacgtaaa      300

agcctgttcg gtatgtaccg taatccgtac accgatgaca cgtctgtgaa gggtctggat      360

cgtaccgttg ccaacacgag catcatcagc catcacggta aggttctggc ggtgaaagaa      420

gatggcttgc cgtacgagct ggacccacgc actctggaaa cccgtggtcg ctttgactat      480

gatggccaag tcaccagcca gacgcacacc gcgcatccga agtatgaccc ggaaaccggt      540

gacctgttgt tctttggctc cgcagccaag ggtgaggcaa cgcctgatat ggcctattac      600

atcgttgata aacatggtaa ggtaacgcat gagacttggt tcgagcaacc gtatggcgcc      660

tttatgcatg attttgctat tacccgcaat tggagcatct tcccgatcat gccggctacc      720

aattcgttga gccgcctgaa agcgaagcag ccgatttaca tgtgggagcc ggaactgggt      780

tcctatattg gtgttctgcc gcgtcgcggt caaggtagcc agatccgctg gctgaaagca      840

ccagcgctgt gggtctttca cgtcgtcaac gcctgggaag tgggcaccaa aatctacatt      900

gaccttatgg agagcgaaat tctgccattc ccgttcccga atagccaaaa tcagccgttc      960

gctcctgaga aagcagtgcc gcgtctgacc cgttgggaga ttgatctgga tagcagcagc     1020

gatgagatta agcgtacgcg tctgcacgac ttctttgcag agatgccgat catggatttt     1080

cgttttgcgc tgcagtgcaa ccgctacggt tttatgggtg tcgatgaccc gcgcaagccg     1140

ctggcgcacc aacaagcgga gaagattttt gcgtacaata gcctgggtat ctgggacaac     1200

caccgtggtg attatgattt gtggtacagc ggcgaagcct cagcggcgca agaaccggct     1260

ttcgttccgc gttctccgac tgcagcggaa ggcgacggtt atctgctgac cgttgtgggc     1320

cgtttggacg agaaccgcag cgacctggtc attctggaca cgcaggacat ccagagcggt     1380

ccggttgcga ccattaaact gccgtttcgc ctgcgtgcgg ccctgcacgg ctgttgggtt     1440

ccgcgtccgt aaaattaggt aaaaaataaa aaatgaatat tcgtccattg catgatcgcg     1500

tgatcgtcaa gcgtaaagaa gttgaaacta aatctgctgg cggcatcgtt ctgaccggct     1560

ctgcagcggc taaatccacc cgcggcgaag tgctggctgt cggcaatggc cgtatccttg     1620

aaaatggcga agtgaagccg ctggatgtga aagttggcga catcgttatt ttcaacgatg     1680

gctacggtgt gaaatctgag aagatcgaca atgaagaagt gttgatcatg tccgaaagcg     1740

acattctggc aattgttgaa gcgtaatccg cgcacgacac tgaacatacg aatttaagga     1800

ataaagataa tggcagctaa agacgtaaaa ttcggtaacg acgctcgtgt gaaaatgctg     1860

cgcggcgtaa acgtactggc agatgcagtg aaagttaccc tcggtccaaa aggccgtaac     1920

gtagttctgg ataaatcttt cggtgcaccg accatcacca aagatggtgt ttccgttgct     1980

cgtgaaatcg aactggaaga caagttcgaa aatatgggtg cgcagatggt gaaagaagtt     2040

gcctctaaag caaacgacgc tgcaggcgac ggtaccacca ctgcaaccgt actggctcag     2100

gctatcatca ctgaaggtct gaaagctgtt gctgcgggca tgaacccgat ggacctgaaa     2160

cgtggtatcg acaaagcggt taccgctgca gttgaagaac tgaaagcgct gtccgtacca     2220

tgctctgact ctaaagcgat tgctcaggtt ggtaccatct ccgctaactc cgacgaaacc     2280

gtaggtaaac tgatcgctga agcgatggac aaagtcggta aagaaggcgt tatcaccgtt     2340

gaagacggta ccggtctgca ggacgaactg gacgtggttg aaggtatgca gttcgaccgt     2400

ggctacctgt ctccttactt catcaacaag ccggaaactg gcgcagtaga actggaaagc     2460

ccgttcatcc tgctggctga caagaaaatc tccaacatcc gcgaaatgct gccggttctg     2520

gaagctgttg ccaaagcagg caaaccgctg ctgatcatcg ctgaagatgt agaaggcgaa     2580

gcgctggcaa ctctggttgt taacaccatg cgtggcatcg tgaaagtcgc tgcggttaaa     2640

gcaccgggct tcggcgatcg tcgtaaagct atgctgcagg atatcgcaac cctgactggc     2700

ggtaccgtga tctctgaaga gatcggtatg gagctggaaa aagcaaccct ggaagacctg     2760

ggtcaggcta aacgtgttgt gatcaacaaa gacaccacca ctatcatcga tggcgtgggt     2820

gaagaagctg caatccaggg ccgtgttgct cagatccgtc agcagattga agaagcaact     2880

tctgactacg accgtgaaaa actgcaggaa cgcgtagcga aactggcagg cggcgttgca     2940

gttatcaaag tgggtgctgc taccgaagtt gaaatgaaag agaaaaaagc acgcgttgaa     3000

gatgccctgc acgcgacccg tgctgcggta gaagaaggcg tggttgctgg tggtggtgtt     3060

gcgctgatcc gcgtagcgtc taaactggct gacctgcgtg gtcagaacga agaccagaac     3120

gtgggtatca aagttgcact gcgtgcaatg gaagctccgc tgcgtcagat cgtattgaac     3180

tgcggcgaag aaccgtctgt tgttgctaac accgttaaag gcggcgacgg caactacggt     3240

tacaacgcag caaccgaaga atacggcaac atgatcgaca tgggtatcct ggatccaacc     3300

aaagtaactc gttctgctct gcagtacgca gcttctgtgg ctggcctgat gatcaccacc     3360

gaatgcatgg ttaccgacct gccgaaaaac gatgcagctg acttaggcgc tgctggcggt     3420

atgggcggca tgggtggcat gggcggcatg atgtaa                               3456


<210>  14
<211>  3423
<212>  DNA
<213>  PC4 (polycistroncic construct containing codon modified isoeugenol monooxygenase of P. nitroreducens Jin 1, groES, groEL)

<400>  14
gaaggagata tacatatggc acgtctgaac cgtaatgatc cgcaactcgt cggcaccctg       60

ttaccaaccc gtattgaggc tgacctgttc gacctggaag tggacggcga aattccgaag      120

tccatcaacg gtaccttcta ccgtaacacg ccggagcctc aggtgacgcc gcagaaattc      180

cacaccttca tcgatggtga cggcatggcg tctgcatttc attttgaaga tggccacgtg      240

gacttcatca gccgctgggt taaaaccgcg cgtttcacgg cggagagact ggcacgtaaa      300

agcctgttcg gtatgtaccg taatccgtac accgatgaca cgtctgtgaa gggtctggat      360

cgtaccgttg ccaacacgag catcatcagc catcacggta aggttctggc ggtgaaagaa      420

gatggcttgc cgtacgagct ggacccacgc actctggaaa cccgtggtcg ctttgactat      480

gatggccaag tcaccagcca gacgcacacc gcgcatccga agtatgaccc ggaaaccggt      540

gacctgttgt tctttggctc cgcagccaag ggtgaggcaa cgcctgatat ggcctattac      600

atcgttgata aacatggtaa ggtaacgcat gagacttggt tcgagcaacc gtatggcgcc      660

tttatgcatg attttgctat tacccgcaat tggagcatct tcccgatcat gccggctacc      720

aattcgttga gccgcctgaa agcgaagcag ccgatttaca tgtgggagcc ggaactgggt      780

tcctatattg gtgttctgcc gcgtcgcggt caaggtagcc agatccgctg gctgaaagca      840

ccagcgctgt gggtctttca cgtcgtcaac gcctgggaag tgggcaccaa aatctacatt      900

gaccttatgg agagcgaaat tctgccattc ccgttcccga atagccaaaa tcagccgttc      960

gctcctgaga aagcagtgcc gcgtctgacc cgttgggaga ttgatctgga tagcagcagc     1020

gatgagatta agcgtacgcg tctgcacgac ttctttgcag agatgccgat catggatttt     1080

cgttttgcgc tgcagtgcaa ccgctacggt tttatgggtg tcgatgaccc gcgcaagccg     1140

ctggcgcacc aacaagcgga gaagattttt gcgtacaata gcctgggtat ctgggacaac     1200

caccgtggtg attatgattt gtggtacagc ggcgaagcct cagcggcgca agaaccggct     1260

ttcgttccgc gttctccgac tgcagcggaa ggcgacggtt atctgctgac cgttgtgggc     1320

cgtttggacg agaaccgcag cgacctggtc attctggaca cgcaggacat ccagagcggt     1380

ccggttgcga ccattaaact gccgtttcgc ctgcgtgcgg ccctgcacgg ctgttgggtt     1440

ccgcgtccgt aagaaggaga tatacatatg aatattcgtc cattgcatga tcgcgtgatc     1500

gtcaagcgta aagaagttga aactaaatct gctggcggca tcgttctgac cggctctgca     1560

gcggctaaat ccacccgcgg cgaagtgctg gctgtcggca atggccgtat ccttgaaaat     1620

ggcgaagtga agccgctgga tgtgaaagtt ggcgacatcg ttattttcaa cgatggctac     1680

ggtgtgaaat ctgagaagat cgacaatgaa gaagtgttga tcatgtccga aagcgacatt     1740

ctggcaattg ttgaagcgta agaaggagat atacatatgg cagctaaaga cgtaaaattc     1800

ggtaacgacg ctcgtgtgaa aatgctgcgc ggcgtaaacg tactggcaga tgcagtgaaa     1860

gttaccctcg gtccaaaagg ccgtaacgta gttctggata aatctttcgg tgcaccgacc     1920

atcaccaaag atggtgtttc cgttgctcgt gaaatcgaac tggaagacaa gttcgaaaat     1980

atgggtgcgc agatggtgaa agaagttgcc tctaaagcaa acgacgctgc aggcgacggt     2040

accaccactg caaccgtact ggctcaggct atcatcactg aaggtctgaa agctgttgct     2100

gcgggcatga acccgatgga cctgaaacgt ggtatcgaca aagcggttac cgctgcagtt     2160

gaagaactga aagcgctgtc cgtaccatgc tctgactcta aagcgattgc tcaggttggt     2220

accatctccg ctaactccga cgaaaccgta ggtaaactga tcgctgaagc gatggacaaa     2280

gtcggtaaag aaggcgttat caccgttgaa gacggtaccg gtctgcagga cgaactggac     2340

gtggttgaag gtatgcagtt cgaccgtggc tacctgtctc cttacttcat caacaagccg     2400

gaaactggcg cagtagaact ggaaagcccg ttcatcctgc tggctgacaa gaaaatctcc     2460

aacatccgcg aaatgctgcc ggttctggaa gctgttgcca aagcaggcaa accgctgctg     2520

atcatcgctg aagatgtaga aggcgaagcg ctggcaactc tggttgttaa caccatgcgt     2580

ggcatcgtga aagtcgctgc ggttaaagca ccgggcttcg gcgatcgtcg taaagctatg     2640

ctgcaggata tcgcaaccct gactggcggt accgtgatct ctgaagagat cggtatggag     2700

ctggaaaaag caaccctgga agacctgggt caggctaaac gtgttgtgat caacaaagac     2760

accaccacta tcatcgatgg cgtgggtgaa gaagctgcaa tccagggccg tgttgctcag     2820

atccgtcagc agattgaaga agcaacttct gactacgacc gtgaaaaact gcaggaacgc     2880

gtagcgaaac tggcaggcgg cgttgcagtt atcaaagtgg gtgctgctac cgaagttgaa     2940

atgaaagaga aaaaagcacg cgttgaagat gccctgcacg cgacccgtgc tgcggtagaa     3000

gaaggcgtgg ttgctggtgg tggtgttgcg ctgatccgcg tagcgtctaa actggctgac     3060

ctgcgtggtc agaacgaaga ccagaacgtg ggtatcaaag ttgcactgcg tgcaatggaa     3120

gctccgctgc gtcagatcgt attgaactgc ggcgaagaac cgtctgttgt tgctaacacc     3180

gttaaaggcg gcgacggcaa ctacggttac aacgcagcaa ccgaagaata cggcaacatg     3240

atcgacatgg gtatcctgga tccaaccaaa gtaactcgtt ctgctctgca gtacgcagct     3300

tctgtggctg gcctgatgat caccaccgaa tgcatggtta ccgacctgcc gaaaaacgat     3360

gcagctgact taggcgctgc tggcggtatg ggcggcatgg gtggcatggg cggcatgatg     3420

taa                                                                   3423


<210>  15
<211>  3456
<212>  DNA
<213>  PC5 (polycistroncic construct containing codon modified isoeugenol monooxygenase of P. nitroreducens Jin 1, groES, groEL)

<400>  15
gaaggagata tacatatggc acgtctgaac cgtaatgatc cgcaactcgt cggcaccctg       60

ttaccaaccc gtattgaggc tgacctgttc gacctggaag tggacggcga aattccgaag      120

tccatcaacg gtaccttcta ccgtaacacg ccggagcctc aggtgacgcc gcagaaattc      180

cacaccttca tcgatggtga cggcatggcg tctgcatttc attttgaaga tggccacgtg      240

gacttcatca gccgctgggt taaaaccgcg cgtttcacgg cggagagact ggcacgtaaa      300

agcctgttcg gtatgtaccg taatccgtac accgatgaca cgtctgtgaa gggtctggat      360

cgtaccgttg ccaacacgag catcatcagc catcacggta aggttctggc ggtgaaagaa      420

gatggcttgc cgtacgagct ggacccacgc actctggaaa cccgtggtcg ctttgactat      480

gatggccaag tcaccagcca gacgcacacc gcgcatccga agtatgaccc ggaaaccggt      540

gacctgttgt tctttggctc cgcagccaag ggtgaggcaa cgcctgatat ggcctattac      600

atcgttgata aacatggtaa ggtaacgcat gagacttggt tcgagcaacc gtatggcgcc      660

tttatgcatg attttgctat tacccgcaat tggagcatct tcccgatcat gccggctacc      720

aattcgttga gccgcctgaa agcgaagcag ccgatttaca tgtgggagcc ggaactgggt      780

tcctatattg gtgttctgcc gcgtcgcggt caaggtagcc agatccgctg gctgaaagca      840

ccagcgctgt gggtctttca cgtcgtcaac gcctgggaag tgggcaccaa aatctacatt      900

gaccttatgg agagcgaaat tctgccattc ccgttcccga atagccaaaa tcagccgttc      960

gctcctgaga aagcagtgcc gcgtctgacc cgttgggaga ttgatctgga tagcagcagc     1020

gatgagatta agcgtacgcg tctgcacgac ttctttgcag agatgccgat catggatttt     1080

cgttttgcgc tgcagtgcaa ccgctacggt tttatgggtg tcgatgaccc gcgcaagccg     1140

ctggcgcacc aacaagcgga gaagattttt gcgtacaata gcctgggtat ctgggacaac     1200

caccgtggtg attatgattt gtggtacagc ggcgaagcct cagcggcgca agaaccggct     1260

ttcgttccgc gttctccgac tgcagcggaa ggcgacggtt atctgctgac cgttgtgggc     1320

cgtttggacg agaaccgcag cgacctggtc attctggaca cgcaggacat ccagagcggt     1380

ccggttgcga ccattaaact gccgtttcgc ctgcgtgcgg ccctgcacgg ctgttgggtt     1440

ccgcgtccgt aaaagatagc aaaaaataaa aaatgaatat tcgtccattg catgatcgcg     1500

tgatcgtcaa gcgtaaagaa gttgaaacta aatctgctgg cggcatcgtt ctgaccggct     1560

ctgcagcggc taaatccacc cgcggcgaag tgctggctgt cggcaatggc cgtatccttg     1620

aaaatggcga agtgaagccg ctggatgtga aagttggcga catcgttatt ttcaacgatg     1680

gctacggtgt gaaatctgag aagatcgaca atgaagaagt gttgatcatg tccgaaagcg     1740

acattctggc aattgttgaa gcgtaatccg cgcacgacac tgaacatacg aatttaagga     1800

ataaagataa tggcagctaa agacgtaaaa ttcggtaacg acgctcgtgt gaaaatgctg     1860

cgcggcgtaa acgtactggc agatgcagtg aaagttaccc tcggtccaaa aggccgtaac     1920

gtagttctgg ataaatcttt cggtgcaccg accatcacca aagatggtgt ttccgttgct     1980

cgtgaaatcg aactggaaga caagttcgaa aatatgggtg cgcagatggt gaaagaagtt     2040

gcctctaaag caaacgacgc tgcaggcgac ggtaccacca ctgcaaccgt actggctcag     2100

gctatcatca ctgaaggtct gaaagctgtt gctgcgggca tgaacccgat ggacctgaaa     2160

cgtggtatcg acaaagcggt taccgctgca gttgaagaac tgaaagcgct gtccgtacca     2220

tgctctgact ctaaagcgat tgctcaggtt ggtaccatct ccgctaactc cgacgaaacc     2280

gtaggtaaac tgatcgctga agcgatggac aaagtcggta aagaaggcgt tatcaccgtt     2340

gaagacggta ccggtctgca ggacgaactg gacgtggttg aaggtatgca gttcgaccgt     2400

ggctacctgt ctccttactt catcaacaag ccggaaactg gcgcagtaga actggaaagc     2460

ccgttcatcc tgctggctga caagaaaatc tccaacatcc gcgaaatgct gccggttctg     2520

gaagctgttg ccaaagcagg caaaccgctg ctgatcatcg ctgaagatgt agaaggcgaa     2580

gcgctggcaa ctctggttgt taacaccatg cgtggcatcg tgaaagtcgc tgcggttaaa     2640

gcaccgggct tcggcgatcg tcgtaaagct atgctgcagg atatcgcaac cctgactggc     2700

ggtaccgtga tctctgaaga gatcggtatg gagctggaaa aagcaaccct ggaagacctg     2760

ggtcaggcta aacgtgttgt gatcaacaaa gacaccacca ctatcatcga tggcgtgggt     2820

gaagaagctg caatccaggg ccgtgttgct cagatccgtc agcagattga agaagcaact     2880

tctgactacg accgtgaaaa actgcaggaa cgcgtagcga aactggcagg cggcgttgca     2940

gttatcaaag tgggtgctgc taccgaagtt gaaatgaaag agaaaaaagc acgcgttgaa     3000

gatgccctgc acgcgacccg tgctgcggta gaagaaggcg tggttgctgg tggtggtgtt     3060

gcgctgatcc gcgtagcgtc taaactggct gacctgcgtg gtcagaacga agaccagaac     3120

gtgggtatca aagttgcact gcgtgcaatg gaagctccgc tgcgtcagat cgtattgaac     3180

tgcggcgaag aaccgtctgt tgttgctaac accgttaaag gcggcgacgg caactacggt     3240

tacaacgcag caaccgaaga atacggcaac atgatcgaca tgggtatcct ggatccaacc     3300

aaagtaactc gttctgctct gcagtacgca gcttctgtgg ctggcctgat gatcaccacc     3360

gaatgcatgg ttaccgacct gccgaaaaac gatgcagctg acttaggcgc tgctggcggt     3420

atgggcggca tgggtggcat gggcggcatg atgtaa                               3456


<210>  16
<211>  5424
<212>  DNA
<213>  pIEM2_C154_T222_A1318


<220>
<221>  Ori_p15a
<222>  (1)..(827)

<220>
<221>  Term_rpoC
<222>  (1009)..(1128)

<220>
<221>  Term_bla
<222>  (1129)..(1429)

<220>
<221>  P_T7_Inducible
<222>  (1456)..(1497)

<220>
<221>  LacO1
<222>  (1477)..(1497)

<220>
<221>  IEM_C154_T222_A1318
<222>  (1528)..(2997)

<220>
<221>  Term_T7
<222>  (3002)..(3049)

<220>
<221>  P_Amp
<222>  (3322)..(3439)

<220>
<221>  Kanamycin-r
<222>  (3450)..(4259)

<220>
<221>  P_lacI
<222>  (4260)..(4341)

<220>
<221>  lacI
<222>  (4342)..(5424)

<400>  16
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgag gatccgaatt cgagctcccc     3000

cctagcataa ccccttgggg cctctaaacg ggtcttgagg ggttttttgc ccctgagacg     3060

cgtcaatcga gttcgtacct aagggcgaca ccccctaatt agcccgggcg aaaggcccag     3120

tctttcgact gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcatgggg     3180

agtccccaca ctaccatcgg cgctacggcg tttcacttct gagttcggca tggggtcagg     3240

tgggaccacc gcgctactgc cgccaggcaa acaaggggtg ttatgagcca tattcaggta     3300

taaatgggct cgcgataatg ttcagaattg gttaattggt tgtaacactg acccctattt     3360

gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa     3420

tgcttcaata atattgaaaa aggaagaata tgagccatat tcaacgggaa acgtcgaggc     3480

cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct cgcgataatg     3540

tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg ccagagttgt     3600

ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg gtcagactaa     3660

actggctgac ggaatttatg ccacttccga ccatcaagca ttttatccgt actcctgatg     3720

atgcatggtt actcaccact gcgatccccg gaaaaacagc gttccaggta ttagaagaat     3780

atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc cggttgcact     3840

cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgcctc gctcaggcgc     3900

aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag cgtaatggct     3960

ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca ccggattcag     4020

tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg aaattaatag     4080

gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt gccatcctat     4140

ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa aaatatggta     4200

ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag tttttctaag     4260

cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg catgatagcg cccggaagag     4320

agtcaattca gggtggtgaa tatgaaacca gtaacgttat acgatgtcgc agagtatgcc     4380

ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa     4440

acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca     4500

caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg     4560

cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc     4620

gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat     4680

cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc     4740

attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag     4800

acacccatca acagtattat tttctcccat gaggacggta cgcgactggg cgtggagcat     4860

ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg     4920

gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata     4980

gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg     5040

aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca     5100

atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac     5160

gacgataccg aagatagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt     5220

cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg     5280

aagggcaatc agctgttgcc agtctcactg gtgaaaagaa aaaccaccct ggcgcccaat     5340

acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt     5400

tcccgactgg aaagcgggca gtga                                            5424


<210>  17
<211>  7557
<212>  DNA
<213>  construct trc/+term

<400>  17
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tcctgtccat gactcggtga caattaatta     3060

tccggctcgt ataatgtgtg gaattgtgag cggataacaa ttccctctag aaataatttt     3120

gtttaacttt taaaggagag ttatcaatga atattcgtcc attgcatgat cgcgtgatcg     3180

tcaagcgtaa agaagttgaa actaaatctg ctggcggcat cgttctgacc ggctctgcag     3240

cggctaaatc cacccgcggc gaagtgctgg ctgtcggcaa tggccgtatc cttgaaaatg     3300

gcgaagtgaa gccgctggat gtgaaagttg gcgacatcgt tattttcaac gatggctacg     3360

gtgtgaaatc tgagaagatc gacaatgaag aagtgttgat catgtccgaa agcgacattc     3420

tggcaattgt tgaagcgtaa tccgcgcacg acactgaaca tacgaattta aggaataaag     3480

ataatggcag ctaaagacgt aaaattcggt aacgacgctc gtgtgaaaat gctgcgcggc     3540

gtaaacgtac tggcagatgc agtgaaagtt accctcggtc caaaaggccg taacgtagtt     3600

ctggataaat ctttcggtgc accgaccatc accaaagatg gtgtttccgt tgctcgtgaa     3660

atcgaactgg aagacaagtt cgaaaatatg ggtgcgcaga tggtgaaaga agttgcctct     3720

aaagcaaacg acgctgcagg cgacggtacc accactgcaa ccgtactggc tcaggctatc     3780

atcactgaag gtctgaaagc tgttgctgcg ggcatgaacc cgatggacct gaaacgtggt     3840

atcgacaaag cggttaccgc tgcagttgaa gaactgaaag cgctgtccgt accatgctct     3900

gactctaaag cgattgctca ggttggtacc atctccgcta actccgacga aaccgtaggt     3960

aaactgatcg ctgaagcgat ggacaaagtc ggtaaagaag gcgttatcac cgttgaagac     4020

ggtaccggtc tgcaggacga actggacgtg gttgaaggta tgcagttcga ccgtggctac     4080

ctgtctcctt acttcatcaa caagccggaa actggcgcag tagaactgga aagcccgttc     4140

atcctgctgg ctgacaagaa aatctccaac atccgcgaaa tgctgccggt tctggaagct     4200

gttgccaaag caggcaaacc gctgctgatc atcgctgaag atgtagaagg cgaagcgctg     4260

gcaactctgg ttgttaacac catgcgtggc atcgtgaaag tcgctgcggt taaagcaccg     4320

ggcttcggcg atcgtcgtaa agctatgctg caggatatcg caaccctgac tggcggtacc     4380

gtgatctctg aagagatcgg tatggagctg gaaaaagcaa ccctggaaga cctgggtcag     4440

gctaaacgtg ttgtgatcaa caaagacacc accactatca tcgatggcgt gggtgaagaa     4500

gctgcaatcc agggccgtgt tgctcagatc cgtcagcaga ttgaagaagc aacttctgac     4560

tacgaccgtg aaaaactgca ggaacgcgta gcgaaactgg caggcggcgt tgcagttatc     4620

aaagtgggtg ctgctaccga agttgaaatg aaagagaaaa aagcacgcgt tgaagatgcc     4680

ctgcacgcga cccgtgctgc ggtagaagaa ggcgtggttg ctggtggtgg tgttgcgctg     4740

atccgcgtag cgtctaaact ggctgacctg cgtggtcaga acgaagacca gaacgtgggt     4800

atcaaagttg cactgcgtgc aatggaagct ccgctgcgtc agatcgtatt gaactgcggc     4860

gaagaaccgt ctgttgttgc taacaccgtt aaaggcggcg acggcaacta cggttacaac     4920

gcagcaaccg aagaatacgg caacatgatc gacatgggta tcctggatcc aaccaaagta     4980

actcgttctg ctctgcagta cgcagcttct gtggctggcc tgatgatcac caccgaatgc     5040

atggttaccg acctgccgaa aaacgatgca gctgacttag gcgctgctgg cggtatgggc     5100

ggcatgggtg gcatgggcgg catgatgtaa ccccctagca taaccccttg gggcctctaa     5160

acgggtcttg aggggttttt tgcccctgag acgcgtcaat cgagttcgta cctaagggcg     5220

acacccccta attagcccgg gcgaaaggcc cagtctttcg actgagcctt tcgttttatt     5280

tgatgcctgg cagttcccta ctctcgcatg gggagtcccc acactaccat cggcgctacg     5340

gcgtttcact tctgagttcg gcatggggtc aggtgggacc accgcgctac tgccgccagg     5400

caaacaaggg gtgttatgag ccatattcag gtataaatgg gctcgcgata atgttcagaa     5460

ttggttaatt ggttgtaaca ctgaccccta tttgtttatt tttctaaata cattcaaata     5520

tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga     5580

atatgagcca tattcaacgg gaaacgtcga ggccgcgatt aaattccaac atggatgctg     5640

atttatatgg gtataaatgg gctcgcgata atgtcgggca atcaggtgcg acaatctatc     5700

gcttgtatgg gaagcccgat gcgccagagt tgtttctgaa acatggcaaa ggtagcgttg     5760

ccaatgatgt tacagatgag atggtcagac taaactggct gacggaattt atgccacttc     5820

cgaccatcaa gcattttatc cgtactcctg atgatgcatg gttactcacc actgcgatcc     5880

ccggaaaaac agcgttccag gtattagaag aatatcctga ttcaggtgaa aatattgttg     5940

atgcgctggc agtgttcctg cgccggttgc actcgattcc tgtttgtaat tgtcctttta     6000

acagcgatcg cgtatttcgc ctcgctcagg cgcaatcacg aatgaataac ggtttggttg     6060

atgcgagtga ttttgatgac gagcgtaatg gctggcctgt tgaacaagtc tggaaagaaa     6120

tgcataaact tttgccattc tcaccggatt cagtcgtcac tcatggtgat ttctcacttg     6180

ataaccttat ttttgacgag gggaaattaa taggttgtat tgatgttgga cgagtcggaa     6240

tcgcagaccg ataccaggat cttgccatcc tatggaactg cctcggtgag ttttctcctt     6300

cattacagaa acggcttttt caaaaatatg gtattgataa tcctgatatg aataaattgc     6360

agtttcattt gatgctcgat gagtttttct aagcggcgcg ccatcgaatg gcgcaaaacc     6420

tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatatgaaa     6480

ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc     6540

gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg     6600

gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg     6660

ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg     6720

attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc     6780

ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg     6840

atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat     6900

gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc     6960

catgaggacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc     7020

gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat     7080

aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc     7140

atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg     7200

ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg     7260

cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagatag ctcatgttat     7320

atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac     7380

cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gccagtctca     7440

ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg     7500

gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtga        7557


<210>  18
<211>  7556
<212>  DNA
<213>  construct tac/+term

<400>  18
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tcctgtccat gactcggtga caattaatca     3060

tcggctcgta taatgtgtgg aattgtgagc ggataacaat tccctctaga aataattttg     3120

tttaactttt aaaggagagt tatcaatgaa tattcgtcca ttgcatgatc gcgtgatcgt     3180

caagcgtaaa gaagttgaaa ctaaatctgc tggcggcatc gttctgaccg gctctgcagc     3240

ggctaaatcc acccgcggcg aagtgctggc tgtcggcaat ggccgtatcc ttgaaaatgg     3300

cgaagtgaag ccgctggatg tgaaagttgg cgacatcgtt attttcaacg atggctacgg     3360

tgtgaaatct gagaagatcg acaatgaaga agtgttgatc atgtccgaaa gcgacattct     3420

ggcaattgtt gaagcgtaat ccgcgcacga cactgaacat acgaatttaa ggaataaaga     3480

taatggcagc taaagacgta aaattcggta acgacgctcg tgtgaaaatg ctgcgcggcg     3540

taaacgtact ggcagatgca gtgaaagtta ccctcggtcc aaaaggccgt aacgtagttc     3600

tggataaatc tttcggtgca ccgaccatca ccaaagatgg tgtttccgtt gctcgtgaaa     3660

tcgaactgga agacaagttc gaaaatatgg gtgcgcagat ggtgaaagaa gttgcctcta     3720

aagcaaacga cgctgcaggc gacggtacca ccactgcaac cgtactggct caggctatca     3780

tcactgaagg tctgaaagct gttgctgcgg gcatgaaccc gatggacctg aaacgtggta     3840

tcgacaaagc ggttaccgct gcagttgaag aactgaaagc gctgtccgta ccatgctctg     3900

actctaaagc gattgctcag gttggtacca tctccgctaa ctccgacgaa accgtaggta     3960

aactgatcgc tgaagcgatg gacaaagtcg gtaaagaagg cgttatcacc gttgaagacg     4020

gtaccggtct gcaggacgaa ctggacgtgg ttgaaggtat gcagttcgac cgtggctacc     4080

tgtctcctta cttcatcaac aagccggaaa ctggcgcagt agaactggaa agcccgttca     4140

tcctgctggc tgacaagaaa atctccaaca tccgcgaaat gctgccggtt ctggaagctg     4200

ttgccaaagc aggcaaaccg ctgctgatca tcgctgaaga tgtagaaggc gaagcgctgg     4260

caactctggt tgttaacacc atgcgtggca tcgtgaaagt cgctgcggtt aaagcaccgg     4320

gcttcggcga tcgtcgtaaa gctatgctgc aggatatcgc aaccctgact ggcggtaccg     4380

tgatctctga agagatcggt atggagctgg aaaaagcaac cctggaagac ctgggtcagg     4440

ctaaacgtgt tgtgatcaac aaagacacca ccactatcat cgatggcgtg ggtgaagaag     4500

ctgcaatcca gggccgtgtt gctcagatcc gtcagcagat tgaagaagca acttctgact     4560

acgaccgtga aaaactgcag gaacgcgtag cgaaactggc aggcggcgtt gcagttatca     4620

aagtgggtgc tgctaccgaa gttgaaatga aagagaaaaa agcacgcgtt gaagatgccc     4680

tgcacgcgac ccgtgctgcg gtagaagaag gcgtggttgc tggtggtggt gttgcgctga     4740

tccgcgtagc gtctaaactg gctgacctgc gtggtcagaa cgaagaccag aacgtgggta     4800

tcaaagttgc actgcgtgca atggaagctc cgctgcgtca gatcgtattg aactgcggcg     4860

aagaaccgtc tgttgttgct aacaccgtta aaggcggcga cggcaactac ggttacaacg     4920

cagcaaccga agaatacggc aacatgatcg acatgggtat cctggatcca accaaagtaa     4980

ctcgttctgc tctgcagtac gcagcttctg tggctggcct gatgatcacc accgaatgca     5040

tggttaccga cctgccgaaa aacgatgcag ctgacttagg cgctgctggc ggtatgggcg     5100

gcatgggtgg catgggcggc atgatgtaac cccctagcat aaccccttgg ggcctctaaa     5160

cgggtcttga ggggtttttt gcccctgaga cgcgtcaatc gagttcgtac ctaagggcga     5220

caccccctaa ttagcccggg cgaaaggccc agtctttcga ctgagccttt cgttttattt     5280

gatgcctggc agttccctac tctcgcatgg ggagtcccca cactaccatc ggcgctacgg     5340

cgtttcactt ctgagttcgg catggggtca ggtgggacca ccgcgctact gccgccaggc     5400

aaacaagggg tgttatgagc catattcagg tataaatggg ctcgcgataa tgttcagaat     5460

tggttaattg gttgtaacac tgacccctat ttgtttattt ttctaaatac attcaaatat     5520

gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagaa     5580

tatgagccat attcaacggg aaacgtcgag gccgcgatta aattccaaca tggatgctga     5640

tttatatggg tataaatggg ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg     5700

cttgtatggg aagcccgatg cgccagagtt gtttctgaaa catggcaaag gtagcgttgc     5760

caatgatgtt acagatgaga tggtcagact aaactggctg acggaattta tgccacttcc     5820

gaccatcaag cattttatcc gtactcctga tgatgcatgg ttactcacca ctgcgatccc     5880

cggaaaaaca gcgttccagg tattagaaga atatcctgat tcaggtgaaa atattgttga     5940

tgcgctggca gtgttcctgc gccggttgca ctcgattcct gtttgtaatt gtccttttaa     6000

cagcgatcgc gtatttcgcc tcgctcaggc gcaatcacga atgaataacg gtttggttga     6060

tgcgagtgat tttgatgacg agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat     6120

gcataaactt ttgccattct caccggattc agtcgtcact catggtgatt tctcacttga     6180

taaccttatt tttgacgagg ggaaattaat aggttgtatt gatgttggac gagtcggaat     6240

cgcagaccga taccaggatc ttgccatcct atggaactgc ctcggtgagt tttctccttc     6300

attacagaaa cggctttttc aaaaatatgg tattgataat cctgatatga ataaattgca     6360

gtttcatttg atgctcgatg agtttttcta agcggcgcgc catcgaatgg cgcaaaacct     6420

ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatatgaaac     6480

cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg     6540

tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg     6600

cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc     6660

tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga     6720

ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg     6780

gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga     6840

tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg     6900

ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc     6960

atgaggacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg     7020

cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata     7080

aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca     7140

tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc     7200

tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc     7260

gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagatagc tcatgttata     7320

tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc     7380

gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg ccagtctcac     7440

tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg     7500

ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtga         7556


<210>  19
<211>  7544
<212>  DNA
<213>  construct T7/+term

<400>  19
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tcctgtccat gactcggtaa tacgactcac     3060

tataggggaa ttgtgagcgg ataacaattc cctctagaaa taattttgtt taacttttaa     3120

aggagagtta tcaatgaata ttcgtccatt gcatgatcgc gtgatcgtca agcgtaaaga     3180

agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg ctaaatccac     3240

ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg aagtgaagcc     3300

gctggatgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg tgaaatctga     3360

gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg caattgttga     3420

agcgtaatcc gcgcacgaca ctgaacatac gaatttaagg aataaagata atggcagcta     3480

aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta aacgtactgg     3540

cagatgcagt gaaagttacc ctcggtccaa aaggccgtaa cgtagttctg gataaatctt     3600

tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc gaactggaag     3660

acaagttcga aaatatgggt gcgcagatgg tgaaagaagt tgcctctaaa gcaaacgacg     3720

ctgcaggcga cggtaccacc actgcaaccg tactggctca ggctatcatc actgaaggtc     3780

tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc gacaaagcgg     3840

ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc atgctctgac tctaaagcga     3900

ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa ctgatcgctg     3960

aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt accggtctgc     4020

aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg tctccttact     4080

tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc ctgctggctg     4140

acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagctgtt gccaaagcag     4200

gcaaaccgct gctgatcatc gctgaagatg tagaaggcga agcgctggca actctggttg     4260

ttaacaccat gcgtggcatc gtgaaagtcg ctgcggttaa agcaccgggc ttcggcgatc     4320

gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgtg atctctgaag     4380

agatcggtat ggagctggaa aaagcaaccc tggaagacct gggtcaggct aaacgtgttg     4440

tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct gcaatccagg     4500

gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgactac gaccgtgaaa     4560

aactgcagga acgcgtagcg aaactggcag gcggcgttgc agttatcaaa gtgggtgctg     4620

ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agatgccctg cacgcgaccc     4680

gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc cgcgtagcgt     4740

ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc aaagttgcac     4800

tgcgtgcaat ggaagctccg ctgcgtcaga tcgtattgaa ctgcggcgaa gaaccgtctg     4860

ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca gcaaccgaag     4920

aatacggcaa catgatcgac atgggtatcc tggatccaac caaagtaact cgttctgctc     4980

tgcagtacgc agcttctgtg gctggcctga tgatcaccac cgaatgcatg gttaccgacc     5040

tgccgaaaaa cgatgcagct gacttaggcg ctgctggcgg tatgggcggc atgggtggca     5100

tgggcggcat gatgtaaccc cctagcataa ccccttgggg cctctaaacg ggtcttgagg     5160

ggttttttgc ccctgagacg cgtcaatcga gttcgtacct aagggcgaca ccccctaatt     5220

agcccgggcg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag     5280

ttccctactc tcgcatgggg agtccccaca ctaccatcgg cgctacggcg tttcacttct     5340

gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa acaaggggtg     5400

ttatgagcca tattcaggta taaatgggct cgcgataatg ttcagaattg gttaattggt     5460

tgtaacactg acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat     5520

gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaata tgagccatat     5580

tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta     5640

taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa     5700

gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac     5760

agatgagatg gtcagactaa actggctgac ggaatttatg ccacttccga ccatcaagca     5820

ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc     5880

gttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt     5940

gttcctgcgc cggttgcact cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt     6000

atttcgcctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt     6060

tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt     6120

gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt     6180

tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata     6240

ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg     6300

gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat     6360

gctcgatgag tttttctaag cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg     6420

catgatagcg cccggaagag agtcaattca gggtggtgaa tatgaaacca gtaacgttat     6480

acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg     6540

ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt     6600

acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg     6660

ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg     6720

ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct     6780

gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc     6840

cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat     6900

ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaggacggta     6960

cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg     7020

gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc     7080

gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc     7140

aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg     7200

atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg     7260

atatctcggt agtgggatac gacgataccg aagatagctc atgttatatc ccgccgttaa     7320

ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac     7380

tctctcaggg ccaggcggtg aagggcaatc agctgttgcc agtctcactg gtgaaaagaa     7440

aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     7500

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtga                      7544


<210>  20
<211>  7550
<212>  DNA
<213>  construct T5/+term

<400>  20
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tcctgtccat gactcggaaa tcataaaaaa     3060

tttatttgct ttgtgagcgg ataacaatta taataccctc tagaaataat tttgtttaac     3120

ttttaaagga gagttatcaa tgaatattcg tccattgcat gatcgcgtga tcgtcaagcg     3180

taaagaagtt gaaactaaat ctgctggcgg catcgttctg accggctctg cagcggctaa     3240

atccacccgc ggcgaagtgc tggctgtcgg caatggccgt atccttgaaa atggcgaagt     3300

gaagccgctg gatgtgaaag ttggcgacat cgttattttc aacgatggct acggtgtgaa     3360

atctgagaag atcgacaatg aagaagtgtt gatcatgtcc gaaagcgaca ttctggcaat     3420

tgttgaagcg taatccgcgc acgacactga acatacgaat ttaaggaata aagataatgg     3480

cagctaaaga cgtaaaattc ggtaacgacg ctcgtgtgaa aatgctgcgc ggcgtaaacg     3540

tactggcaga tgcagtgaaa gttaccctcg gtccaaaagg ccgtaacgta gttctggata     3600

aatctttcgg tgcaccgacc atcaccaaag atggtgtttc cgttgctcgt gaaatcgaac     3660

tggaagacaa gttcgaaaat atgggtgcgc agatggtgaa agaagttgcc tctaaagcaa     3720

acgacgctgc aggcgacggt accaccactg caaccgtact ggctcaggct atcatcactg     3780

aaggtctgaa agctgttgct gcgggcatga acccgatgga cctgaaacgt ggtatcgaca     3840

aagcggttac cgctgcagtt gaagaactga aagcgctgtc cgtaccatgc tctgactcta     3900

aagcgattgc tcaggttggt accatctccg ctaactccga cgaaaccgta ggtaaactga     3960

tcgctgaagc gatggacaaa gtcggtaaag aaggcgttat caccgttgaa gacggtaccg     4020

gtctgcagga cgaactggac gtggttgaag gtatgcagtt cgaccgtggc tacctgtctc     4080

cttacttcat caacaagccg gaaactggcg cagtagaact ggaaagcccg ttcatcctgc     4140

tggctgacaa gaaaatctcc aacatccgcg aaatgctgcc ggttctggaa gctgttgcca     4200

aagcaggcaa accgctgctg atcatcgctg aagatgtaga aggcgaagcg ctggcaactc     4260

tggttgttaa caccatgcgt ggcatcgtga aagtcgctgc ggttaaagca ccgggcttcg     4320

gcgatcgtcg taaagctatg ctgcaggata tcgcaaccct gactggcggt accgtgatct     4380

ctgaagagat cggtatggag ctggaaaaag caaccctgga agacctgggt caggctaaac     4440

gtgttgtgat caacaaagac accaccacta tcatcgatgg cgtgggtgaa gaagctgcaa     4500

tccagggccg tgttgctcag atccgtcagc agattgaaga agcaacttct gactacgacc     4560

gtgaaaaact gcaggaacgc gtagcgaaac tggcaggcgg cgttgcagtt atcaaagtgg     4620

gtgctgctac cgaagttgaa atgaaagaga aaaaagcacg cgttgaagat gccctgcacg     4680

cgacccgtgc tgcggtagaa gaaggcgtgg ttgctggtgg tggtgttgcg ctgatccgcg     4740

tagcgtctaa actggctgac ctgcgtggtc agaacgaaga ccagaacgtg ggtatcaaag     4800

ttgcactgcg tgcaatggaa gctccgctgc gtcagatcgt attgaactgc ggcgaagaac     4860

cgtctgttgt tgctaacacc gttaaaggcg gcgacggcaa ctacggttac aacgcagcaa     4920

ccgaagaata cggcaacatg atcgacatgg gtatcctgga tccaaccaaa gtaactcgtt     4980

ctgctctgca gtacgcagct tctgtggctg gcctgatgat caccaccgaa tgcatggtta     5040

ccgacctgcc gaaaaacgat gcagctgact taggcgctgc tggcggtatg ggcggcatgg     5100

gtggcatggg cggcatgatg taacccccta gcataacccc ttggggcctc taaacgggtc     5160

ttgaggggtt ttttgcccct gagacgcgtc aatcgagttc gtacctaagg gcgacacccc     5220

ctaattagcc cgggcgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc     5280

tggcagttcc ctactctcgc atggggagtc cccacactac catcggcgct acggcgtttc     5340

acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaacaa     5400

ggggtgttat gagccatatt caggtataaa tgggctcgcg ataatgttca gaattggtta     5460

attggttgta acactgaccc ctatttgttt atttttctaa atacattcaa atatgtatcc     5520

gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agaatatgag     5580

ccatattcaa cgggaaacgt cgaggccgcg attaaattcc aacatggatg ctgatttata     5640

tgggtataaa tgggctcgcg ataatgtcgg gcaatcaggt gcgacaatct atcgcttgta     5700

tgggaagccc gatgcgccag agttgtttct gaaacatggc aaaggtagcg ttgccaatga     5760

tgttacagat gagatggtca gactaaactg gctgacggaa tttatgccac ttccgaccat     5820

caagcatttt atccgtactc ctgatgatgc atggttactc accactgcga tccccggaaa     5880

aacagcgttc caggtattag aagaatatcc tgattcaggt gaaaatattg ttgatgcgct     5940

ggcagtgttc ctgcgccggt tgcactcgat tcctgtttgt aattgtcctt ttaacagcga     6000

tcgcgtattt cgcctcgctc aggcgcaatc acgaatgaat aacggtttgg ttgatgcgag     6060

tgattttgat gacgagcgta atggctggcc tgttgaacaa gtctggaaag aaatgcataa     6120

acttttgcca ttctcaccgg attcagtcgt cactcatggt gatttctcac ttgataacct     6180

tatttttgac gaggggaaat taataggttg tattgatgtt ggacgagtcg gaatcgcaga     6240

ccgataccag gatcttgcca tcctatggaa ctgcctcggt gagttttctc cttcattaca     6300

gaaacggctt tttcaaaaat atggtattga taatcctgat atgaataaat tgcagtttca     6360

tttgatgctc gatgagtttt tctaagcggc gcgccatcga atggcgcaaa acctttcgcg     6420

gtatggcatg atagcgcccg gaagagagtc aattcagggt ggtgaatatg aaaccagtaa     6480

cgttatacga tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga     6540

accaggccag ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc     6600

tgaattacat tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg     6660

gcgttgccac ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat     6720

ctcgcgccga tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg     6780

aagcctgtaa agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta     6840

actatccgct ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg     6900

cgttatttct tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgagg     6960

acggtacgcg actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt     7020

tagcgggccc attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc     7080

tcactcgcaa tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg     7140

gttttcaaca aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg     7200

ccaacgatca gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg     7260

gtgcggatat ctcggtagtg ggatacgacg ataccgaaga tagctcatgt tatatcccgc     7320

cgttaaccac catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc     7380

tgcaactctc tcagggccag gcggtgaagg gcaatcagct gttgccagtc tcactggtga     7440

aaagaaaaac caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt     7500

cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga                7550


<210>  21
<211>  7544
<212>  DNA
<213>  construct H10/+term

<400>  21
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tcctgtccat gactcggtaa tacgactcac     3060

tacggaagaa ttgtgagcgg ataacaattc cctctagaaa taattttgtt taacttttaa     3120

aggagagtta tcaatgaata ttcgtccatt gcatgatcgc gtgatcgtca agcgtaaaga     3180

agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg ctaaatccac     3240

ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg aagtgaagcc     3300

gctggatgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg tgaaatctga     3360

gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg caattgttga     3420

agcgtaatcc gcgcacgaca ctgaacatac gaatttaagg aataaagata atggcagcta     3480

aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta aacgtactgg     3540

cagatgcagt gaaagttacc ctcggtccaa aaggccgtaa cgtagttctg gataaatctt     3600

tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc gaactggaag     3660

acaagttcga aaatatgggt gcgcagatgg tgaaagaagt tgcctctaaa gcaaacgacg     3720

ctgcaggcga cggtaccacc actgcaaccg tactggctca ggctatcatc actgaaggtc     3780

tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc gacaaagcgg     3840

ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc atgctctgac tctaaagcga     3900

ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa ctgatcgctg     3960

aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt accggtctgc     4020

aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg tctccttact     4080

tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc ctgctggctg     4140

acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagctgtt gccaaagcag     4200

gcaaaccgct gctgatcatc gctgaagatg tagaaggcga agcgctggca actctggttg     4260

ttaacaccat gcgtggcatc gtgaaagtcg ctgcggttaa agcaccgggc ttcggcgatc     4320

gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgtg atctctgaag     4380

agatcggtat ggagctggaa aaagcaaccc tggaagacct gggtcaggct aaacgtgttg     4440

tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct gcaatccagg     4500

gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgactac gaccgtgaaa     4560

aactgcagga acgcgtagcg aaactggcag gcggcgttgc agttatcaaa gtgggtgctg     4620

ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agatgccctg cacgcgaccc     4680

gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc cgcgtagcgt     4740

ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc aaagttgcac     4800

tgcgtgcaat ggaagctccg ctgcgtcaga tcgtattgaa ctgcggcgaa gaaccgtctg     4860

ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca gcaaccgaag     4920

aatacggcaa catgatcgac atgggtatcc tggatccaac caaagtaact cgttctgctc     4980

tgcagtacgc agcttctgtg gctggcctga tgatcaccac cgaatgcatg gttaccgacc     5040

tgccgaaaaa cgatgcagct gacttaggcg ctgctggcgg tatgggcggc atgggtggca     5100

tgggcggcat gatgtaaccc cctagcataa ccccttgggg cctctaaacg ggtcttgagg     5160

ggttttttgc ccctgagacg cgtcaatcga gttcgtacct aagggcgaca ccccctaatt     5220

agcccgggcg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag     5280

ttccctactc tcgcatgggg agtccccaca ctaccatcgg cgctacggcg tttcacttct     5340

gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa acaaggggtg     5400

ttatgagcca tattcaggta taaatgggct cgcgataatg ttcagaattg gttaattggt     5460

tgtaacactg acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat     5520

gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaata tgagccatat     5580

tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta     5640

taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa     5700

gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac     5760

agatgagatg gtcagactaa actggctgac ggaatttatg ccacttccga ccatcaagca     5820

ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc     5880

gttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt     5940

gttcctgcgc cggttgcact cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt     6000

atttcgcctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt     6060

tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt     6120

gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt     6180

tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata     6240

ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg     6300

gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat     6360

gctcgatgag tttttctaag cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg     6420

catgatagcg cccggaagag agtcaattca gggtggtgaa tatgaaacca gtaacgttat     6480

acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg     6540

ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt     6600

acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg     6660

ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg     6720

ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct     6780

gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc     6840

cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat     6900

ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaggacggta     6960

cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg     7020

gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc     7080

gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc     7140

aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg     7200

atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg     7260

atatctcggt agtgggatac gacgataccg aagatagctc atgttatatc ccgccgttaa     7320

ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac     7380

tctctcaggg ccaggcggtg aagggcaatc agctgttgcc agtctcactg gtgaaaagaa     7440

aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     7500

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtga                      7544


<210>  22
<211>  7544
<212>  DNA
<213>  construct H9/+term

<400>  22
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tcctgtccat gactcggtaa tacgactcac     3060

taatactgaa ttgtgagcgg ataacaattc cctctagaaa taattttgtt taacttttaa     3120

aggagagtta tcaatgaata ttcgtccatt gcatgatcgc gtgatcgtca agcgtaaaga     3180

agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg ctaaatccac     3240

ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg aagtgaagcc     3300

gctggatgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg tgaaatctga     3360

gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg caattgttga     3420

agcgtaatcc gcgcacgaca ctgaacatac gaatttaagg aataaagata atggcagcta     3480

aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta aacgtactgg     3540

cagatgcagt gaaagttacc ctcggtccaa aaggccgtaa cgtagttctg gataaatctt     3600

tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc gaactggaag     3660

acaagttcga aaatatgggt gcgcagatgg tgaaagaagt tgcctctaaa gcaaacgacg     3720

ctgcaggcga cggtaccacc actgcaaccg tactggctca ggctatcatc actgaaggtc     3780

tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc gacaaagcgg     3840

ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc atgctctgac tctaaagcga     3900

ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa ctgatcgctg     3960

aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt accggtctgc     4020

aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg tctccttact     4080

tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc ctgctggctg     4140

acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagctgtt gccaaagcag     4200

gcaaaccgct gctgatcatc gctgaagatg tagaaggcga agcgctggca actctggttg     4260

ttaacaccat gcgtggcatc gtgaaagtcg ctgcggttaa agcaccgggc ttcggcgatc     4320

gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgtg atctctgaag     4380

agatcggtat ggagctggaa aaagcaaccc tggaagacct gggtcaggct aaacgtgttg     4440

tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct gcaatccagg     4500

gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgactac gaccgtgaaa     4560

aactgcagga acgcgtagcg aaactggcag gcggcgttgc agttatcaaa gtgggtgctg     4620

ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agatgccctg cacgcgaccc     4680

gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc cgcgtagcgt     4740

ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc aaagttgcac     4800

tgcgtgcaat ggaagctccg ctgcgtcaga tcgtattgaa ctgcggcgaa gaaccgtctg     4860

ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca gcaaccgaag     4920

aatacggcaa catgatcgac atgggtatcc tggatccaac caaagtaact cgttctgctc     4980

tgcagtacgc agcttctgtg gctggcctga tgatcaccac cgaatgcatg gttaccgacc     5040

tgccgaaaaa cgatgcagct gacttaggcg ctgctggcgg tatgggcggc atgggtggca     5100

tgggcggcat gatgtaaccc cctagcataa ccccttgggg cctctaaacg ggtcttgagg     5160

ggttttttgc ccctgagacg cgtcaatcga gttcgtacct aagggcgaca ccccctaatt     5220

agcccgggcg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag     5280

ttccctactc tcgcatgggg agtccccaca ctaccatcgg cgctacggcg tttcacttct     5340

gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa acaaggggtg     5400

ttatgagcca tattcaggta taaatgggct cgcgataatg ttcagaattg gttaattggt     5460

tgtaacactg acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat     5520

gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaata tgagccatat     5580

tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta     5640

taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa     5700

gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac     5760

agatgagatg gtcagactaa actggctgac ggaatttatg ccacttccga ccatcaagca     5820

ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc     5880

gttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt     5940

gttcctgcgc cggttgcact cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt     6000

atttcgcctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt     6060

tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt     6120

gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt     6180

tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata     6240

ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg     6300

gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat     6360

gctcgatgag tttttctaag cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg     6420

catgatagcg cccggaagag agtcaattca gggtggtgaa tatgaaacca gtaacgttat     6480

acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg     6540

ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt     6600

acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg     6660

ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg     6720

ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct     6780

gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc     6840

cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat     6900

ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaggacggta     6960

cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg     7020

gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc     7080

gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc     7140

aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg     7200

atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg     7260

atatctcggt agtgggatac gacgataccg aagatagctc atgttatatc ccgccgttaa     7320

ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac     7380

tctctcaggg ccaggcggtg aagggcaatc agctgttgcc agtctcactg gtgaaaagaa     7440

aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     7500

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtga                      7544


<210>  23
<211>  7545
<212>  DNA
<213>  construct G6/+term

<400>  23
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tccctgtcca tgactcggta atacgactca     3060

ctatttcgga attgtgagcg gataacaatt ccctctagaa ataattttgt ttaactttta     3120

aaggagagtt atcaatgaat attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag     3180

aagttgaaac taaatctgct ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca     3240

cccgcggcga agtgctggct gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc     3300

cgctggatgt gaaagttggc gacatcgtta ttttcaacga tggctacggt gtgaaatctg     3360

agaagatcga caatgaagaa gtgttgatca tgtccgaaag cgacattctg gcaattgttg     3420

aagcgtaatc cgcgcacgac actgaacata cgaatttaag gaataaagat aatggcagct     3480

aaagacgtaa aattcggtaa cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg     3540

gcagatgcag tgaaagttac cctcggtcca aaaggccgta acgtagttct ggataaatct     3600

ttcggtgcac cgaccatcac caaagatggt gtttccgttg ctcgtgaaat cgaactggaa     3660

gacaagttcg aaaatatggg tgcgcagatg gtgaaagaag ttgcctctaa agcaaacgac     3720

gctgcaggcg acggtaccac cactgcaacc gtactggctc aggctatcat cactgaaggt     3780

ctgaaagctg ttgctgcggg catgaacccg atggacctga aacgtggtat cgacaaagcg     3840

gttaccgctg cagttgaaga actgaaagcg ctgtccgtac catgctctga ctctaaagcg     3900

attgctcagg ttggtaccat ctccgctaac tccgacgaaa ccgtaggtaa actgatcgct     3960

gaagcgatgg acaaagtcgg taaagaaggc gttatcaccg ttgaagacgg taccggtctg     4020

caggacgaac tggacgtggt tgaaggtatg cagttcgacc gtggctacct gtctccttac     4080

ttcatcaaca agccggaaac tggcgcagta gaactggaaa gcccgttcat cctgctggct     4140

gacaagaaaa tctccaacat ccgcgaaatg ctgccggttc tggaagctgt tgccaaagca     4200

ggcaaaccgc tgctgatcat cgctgaagat gtagaaggcg aagcgctggc aactctggtt     4260

gttaacacca tgcgtggcat cgtgaaagtc gctgcggtta aagcaccggg cttcggcgat     4320

cgtcgtaaag ctatgctgca ggatatcgca accctgactg gcggtaccgt gatctctgaa     4380

gagatcggta tggagctgga aaaagcaacc ctggaagacc tgggtcaggc taaacgtgtt     4440

gtgatcaaca aagacaccac cactatcatc gatggcgtgg gtgaagaagc tgcaatccag     4500

ggccgtgttg ctcagatccg tcagcagatt gaagaagcaa cttctgacta cgaccgtgaa     4560

aaactgcagg aacgcgtagc gaaactggca ggcggcgttg cagttatcaa agtgggtgct     4620

gctaccgaag ttgaaatgaa agagaaaaaa gcacgcgttg aagatgccct gcacgcgacc     4680

cgtgctgcgg tagaagaagg cgtggttgct ggtggtggtg ttgcgctgat ccgcgtagcg     4740

tctaaactgg ctgacctgcg tggtcagaac gaagaccaga acgtgggtat caaagttgca     4800

ctgcgtgcaa tggaagctcc gctgcgtcag atcgtattga actgcggcga agaaccgtct     4860

gttgttgcta acaccgttaa aggcggcgac ggcaactacg gttacaacgc agcaaccgaa     4920

gaatacggca acatgatcga catgggtatc ctggatccaa ccaaagtaac tcgttctgct     4980

ctgcagtacg cagcttctgt ggctggcctg atgatcacca ccgaatgcat ggttaccgac     5040

ctgccgaaaa acgatgcagc tgacttaggc gctgctggcg gtatgggcgg catgggtggc     5100

atgggcggca tgatgtaacc ccctagcata accccttggg gcctctaaac gggtcttgag     5160

gggttttttg cccctgagac gcgtcaatcg agttcgtacc taagggcgac accccctaat     5220

tagcccgggc gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca     5280

gttccctact ctcgcatggg gagtccccac actaccatcg gcgctacggc gtttcacttc     5340

tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca aacaaggggt     5400

gttatgagcc atattcaggt ataaatgggc tcgcgataat gttcagaatt ggttaattgg     5460

ttgtaacact gacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca     5520

tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagaat atgagccata     5580

ttcaacggga aacgtcgagg ccgcgattaa attccaacat ggatgctgat ttatatgggt     5640

ataaatgggc tcgcgataat gtcgggcaat caggtgcgac aatctatcgc ttgtatggga     5700

agcccgatgc gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta     5760

cagatgagat ggtcagacta aactggctga cggaatttat gccacttccg accatcaagc     5820

attttatccg tactcctgat gatgcatggt tactcaccac tgcgatcccc ggaaaaacag     5880

cgttccaggt attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag     5940

tgttcctgcg ccggttgcac tcgattcctg tttgtaattg tccttttaac agcgatcgcg     6000

tatttcgcct cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt     6060

ttgatgacga gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt     6120

tgccattctc accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt     6180

ttgacgaggg gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat     6240

accaggatct tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac     6300

ggctttttca aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga     6360

tgctcgatga gtttttctaa gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg     6420

gcatgatagc gcccggaaga gagtcaattc agggtggtga atatgaaacc agtaacgtta     6480

tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag     6540

gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc ggagctgaat     6600

tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct gattggcgtt     6660

gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc     6720

gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc     6780

tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat cattaactat     6840

ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt tccggcgtta     6900

tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca tgaggacggt     6960

acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg     7020

ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa atatctcact     7080

cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat gtccggtttt     7140

caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct ggttgccaac     7200

gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg     7260

gatatctcgg tagtgggata cgacgatacc gaagatagct catgttatat cccgccgtta     7320

accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa     7380

ctctctcagg gccaggcggt gaagggcaat cagctgttgc cagtctcact ggtgaaaaga     7440

aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta     7500

atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtga                     7545


<210>  24
<211>  7544
<212>  DNA
<213>  construct C4/+term

<400>  24
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgac tagcataacc ccttggggcc     3000

tctaaacggg tcttgagggg ttttttggca tcctgtccat gactcggtaa tacgactcac     3060

tatcaaggaa ttgtgagcgg ataacaattc cctctagaaa taattttgtt taacttttaa     3120

aggagagtta tcaatgaata ttcgtccatt gcatgatcgc gtgatcgtca agcgtaaaga     3180

agttgaaact aaatctgctg gcggcatcgt tctgaccggc tctgcagcgg ctaaatccac     3240

ccgcggcgaa gtgctggctg tcggcaatgg ccgtatcctt gaaaatggcg aagtgaagcc     3300

gctggatgtg aaagttggcg acatcgttat tttcaacgat ggctacggtg tgaaatctga     3360

gaagatcgac aatgaagaag tgttgatcat gtccgaaagc gacattctgg caattgttga     3420

agcgtaatcc gcgcacgaca ctgaacatac gaatttaagg aataaagata atggcagcta     3480

aagacgtaaa attcggtaac gacgctcgtg tgaaaatgct gcgcggcgta aacgtactgg     3540

cagatgcagt gaaagttacc ctcggtccaa aaggccgtaa cgtagttctg gataaatctt     3600

tcggtgcacc gaccatcacc aaagatggtg tttccgttgc tcgtgaaatc gaactggaag     3660

acaagttcga aaatatgggt gcgcagatgg tgaaagaagt tgcctctaaa gcaaacgacg     3720

ctgcaggcga cggtaccacc actgcaaccg tactggctca ggctatcatc actgaaggtc     3780

tgaaagctgt tgctgcgggc atgaacccga tggacctgaa acgtggtatc gacaaagcgg     3840

ttaccgctgc agttgaagaa ctgaaagcgc tgtccgtacc atgctctgac tctaaagcga     3900

ttgctcaggt tggtaccatc tccgctaact ccgacgaaac cgtaggtaaa ctgatcgctg     3960

aagcgatgga caaagtcggt aaagaaggcg ttatcaccgt tgaagacggt accggtctgc     4020

aggacgaact ggacgtggtt gaaggtatgc agttcgaccg tggctacctg tctccttact     4080

tcatcaacaa gccggaaact ggcgcagtag aactggaaag cccgttcatc ctgctggctg     4140

acaagaaaat ctccaacatc cgcgaaatgc tgccggttct ggaagctgtt gccaaagcag     4200

gcaaaccgct gctgatcatc gctgaagatg tagaaggcga agcgctggca actctggttg     4260

ttaacaccat gcgtggcatc gtgaaagtcg ctgcggttaa agcaccgggc ttcggcgatc     4320

gtcgtaaagc tatgctgcag gatatcgcaa ccctgactgg cggtaccgtg atctctgaag     4380

agatcggtat ggagctggaa aaagcaaccc tggaagacct gggtcaggct aaacgtgttg     4440

tgatcaacaa agacaccacc actatcatcg atggcgtggg tgaagaagct gcaatccagg     4500

gccgtgttgc tcagatccgt cagcagattg aagaagcaac ttctgactac gaccgtgaaa     4560

aactgcagga acgcgtagcg aaactggcag gcggcgttgc agttatcaaa gtgggtgctg     4620

ctaccgaagt tgaaatgaaa gagaaaaaag cacgcgttga agatgccctg cacgcgaccc     4680

gtgctgcggt agaagaaggc gtggttgctg gtggtggtgt tgcgctgatc cgcgtagcgt     4740

ctaaactggc tgacctgcgt ggtcagaacg aagaccagaa cgtgggtatc aaagttgcac     4800

tgcgtgcaat ggaagctccg ctgcgtcaga tcgtattgaa ctgcggcgaa gaaccgtctg     4860

ttgttgctaa caccgttaaa ggcggcgacg gcaactacgg ttacaacgca gcaaccgaag     4920

aatacggcaa catgatcgac atgggtatcc tggatccaac caaagtaact cgttctgctc     4980

tgcagtacgc agcttctgtg gctggcctga tgatcaccac cgaatgcatg gttaccgacc     5040

tgccgaaaaa cgatgcagct gacttaggcg ctgctggcgg tatgggcggc atgggtggca     5100

tgggcggcat gatgtaaccc cctagcataa ccccttgggg cctctaaacg ggtcttgagg     5160

ggttttttgc ccctgagacg cgtcaatcga gttcgtacct aagggcgaca ccccctaatt     5220

agcccgggcg aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggcag     5280

ttccctactc tcgcatgggg agtccccaca ctaccatcgg cgctacggcg tttcacttct     5340

gagttcggca tggggtcagg tgggaccacc gcgctactgc cgccaggcaa acaaggggtg     5400

ttatgagcca tattcaggta taaatgggct cgcgataatg ttcagaattg gttaattggt     5460

tgtaacactg acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat     5520

gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagaata tgagccatat     5580

tcaacgggaa acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta     5640

taaatgggct cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa     5700

gcccgatgcg ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac     5760

agatgagatg gtcagactaa actggctgac ggaatttatg ccacttccga ccatcaagca     5820

ttttatccgt actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc     5880

gttccaggta ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt     5940

gttcctgcgc cggttgcact cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt     6000

atttcgcctc gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt     6060

tgatgacgag cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt     6120

gccattctca ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt     6180

tgacgagggg aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata     6240

ccaggatctt gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg     6300

gctttttcaa aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat     6360

gctcgatgag tttttctaag cggcgcgcca tcgaatggcg caaaaccttt cgcggtatgg     6420

catgatagcg cccggaagag agtcaattca gggtggtgaa tatgaaacca gtaacgttat     6480

acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg     6540

ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt     6600

acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg attggcgttg     6660

ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg     6720

ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct     6780

gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc attaactatc     6840

cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt ccggcgttat     6900

ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat gaggacggta     6960

cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg     7020

gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa tatctcactc     7080

gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg tccggttttc     7140

aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg gttgccaacg     7200

atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg     7260

atatctcggt agtgggatac gacgataccg aagatagctc atgttatatc ccgccgttaa     7320

ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac     7380

tctctcaggg ccaggcggtg aagggcaatc agctgttgcc agtctcactg gtgaaaagaa     7440

aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa     7500

tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtga                      7544


<210>  25
<211>  7405
<212>  DNA
<213>  construct PC1_triple

<400>  25
ttaataagat gatcttcttg agatcgtttt ggtctgcgcg taatctcttg ctctgaaaac       60

gaaaaaaccg ccttgcaggg cggtttttcg aaggttctct gagctaccaa ctctttgaac      120

cgaggtaact ggcttggagg agcgcagtca ccaaaacttg tcctttcagt ttagccttaa      180

ccggcgcatg acttcaagac taactcctct aaatcaatta ccagtggctg ctgccagtgg      240

tgcttttgca tgtctttccg ggttggactc aagacgatag ttaccggata aggcgcagcg      300

gtcggactga acggggggtt cgtgcataca gtccagcttg gagcgaactg cctacccgga      360

actgagtgtc aggcgtggaa tgagacaaac gcggccataa cagcggaatg acaccggtaa      420

accgaaaggc aggaacagga gagcgcacga gggagccgcc agggggaaac gcctggtatc      480

tttatagtcc tgtcgggttt cgccaccact gatttgagcg tcagatttcg tgatgcttgt      540

caggggggcg gagcctatgg aaaaacggct ttgccgcggc cctctcactt ccctgttaag      600

tatcttcctg gcatcttcca ggaaatctcc gccccgttcg taagccattt ccgctcgccg      660

cagtcgaacg accgagcgta gcgagtcagt gagcgaggaa gcggaatata tcctgtatca      720

catattctgc tgacgcaccg gtgcagcctt ttttctcctg ccacatgaag cacttcactg      780

acaccctcat cagtgccaac atagtaagcc agtatacact ccgctagcgc tgaggtcccg      840

cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaaggcg agagtaggga      900

actgccaggc atcaaactaa gcagaaggcc cctgacggat ggcctttttg cgtttctaca      960

aactctttct gtgttgtaaa acgacggcca gtcttaagct cgggccccct gggcggttct     1020

gataacgagt aatcgttaat ccgcaaataa cgtaaaaacc cgcttcggcg ggttttttta     1080

tggggggagt ttagggaaag agcatttgtc agaatattta agggcgcctg tcactttgct     1140

tgatatatga gaattattta accttataaa tgagaaaaaa gcaacgcact ttaaataaga     1200

tacgttgctt tttcgattga tgaacaccta taattaaact attcatctat tatttatgat     1260

tttttgtata tacaatattt ctagtttgtt aaagagaatt aagaaaataa atctcgaaaa     1320

taataaaggg aaaatcagtt tttgatatca aaattataca tgtcaacgat aatacaaaat     1380

ataatacaaa ctataagatg ttatcagtat ttattatgca tttagaataa attttgtgtc     1440

gcccttccgc gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc     1500

ctctagaaat aattttgttt aacttttgaa ggagatatac atatggcaac gtttgaccgc     1560

aatgatccgc agttggcagg aacgatgttc cccacccgaa tagaggcgaa tgtctttgac     1620

cttgaaattg agggcgagat cccacgtgca atcaacggga gcttcttccg caacaccccc     1680

gaacctcagg tcaccccgca acctttccac accttcatcg atggggatgg tttggcgtct     1740

gcttttcatt tcgaagatgg ccatgtcgac tttgtcagcc gttgggtatg tactcctcgc     1800

tttgaagctg agcggtcggc tcgtaaatca ctcttcggta tgtaccgcaa tccgttcact     1860

gatgatccat cggtagaagg tattgatcgt acagtcgcca acaccagtat catcactcat     1920

cacgggaaag tactggccgc aaaggaagat ggactacctt atgagcttga cccccaaacc     1980

ctggaaaccc gaggtcgtta tgattacaag gggcaggtaa ccagccatac acatacagcg     2040

caccctaagt tcgaccccca gacaggtgaa atgttactct tcggctccgc tgctaaaggc     2100

gaacgaacgc ttgatatggc gtactatatt gttgatcgct acggcaaggt gacacatgag     2160

acctggttta agcagcctta cggtgcattc atgcacgact ttgctgtcac gcgcaactgg     2220

tcaatctttc cgatcatgcc tgcgacaaat agccttgagc gtcttaaagc aaagcagccc     2280

atttacatgt gggagcctga gcgaggaagc tatataggag tacttcctcg tcgtggtcag     2340

ggcaaggaca ttcgttggtt ccgtgccccg gcgttgtggg ttttccatgt cgtgaatgct     2400

tgggaggaag ggaatagaat tctgattgac ttgatggaaa gtgagatttt gccgttccca     2460

ttcccgaact cgcagaacct tccatttgat ccctccaagg ctgttccgcg tctaacccgt     2520

tgggaaattg atctcaatag tggtaacgat gagatgaaac gtacgcagct acacgaatat     2580

tttgcagaaa tgcctatcat ggatttccgt tttgcgctcc aggatcatcg ctacgcctac     2640

atgggggttg acgatcctcg tcgcccctta gctcatcagc aagctgaaaa aatctttgcc     2700

tacaattcgt taggggtttg ggacaaccat cgtaaagatt atgaactttg gtttacggga     2760

aaaatgtctg cagcgcagga accggcgttt gttcctagaa gcccagatgc gcctgagggc     2820

gatggctacc tactcagtgt agtagggcgg ctcgatgaaa atcgtagcga tctagttatc     2880

cttgatacgc aatgtttggc agctgggcct gtggccactg tcaagcttcc cttccgtctc     2940

cgagcagcgt tgcacggttg ttggcagtct aagaactgag aaggagatat acatatgaat     3000

attcgtccat tgcatgatcg cgtgatcgtc aagcgtaaag aagttgaaac taaatctgct     3060

ggcggcatcg ttctgaccgg ctctgcagcg gctaaatcca cccgcggcga agtgctggct     3120

gtcggcaatg gccgtatcct tgaaaatggc gaagtgaagc cgctggatgt gaaagttggc     3180

gacatcgtta ttttcaacga tggctacggt gtgaaatctg agaagatcga caatgaagaa     3240

gtgttgatca tgtccgaaag cgacattctg gcaattgttg aagcgtaatc cgcgcacgac     3300

actgaacata cgaatttaag gaataaagat aatggcagct aaagacgtaa aattcggtaa     3360

cgacgctcgt gtgaaaatgc tgcgcggcgt aaacgtactg gcagatgcag tgaaagttac     3420

cctcggtcca aaaggccgta acgtagttct ggataaatct ttcggtgcac cgaccatcac     3480

caaagatggt gtttccgttg ctcgtgaaat cgaactggaa gacaagttcg aaaatatggg     3540

tgcgcagatg gtgaaagaag ttgcctctaa agcaaacgac gctgcaggcg acggtaccac     3600

cactgcaacc gtactggctc aggctatcat cactgaaggt ctgaaagctg ttgctgcggg     3660

catgaacccg atggacctga aacgtggtat cgacaaagcg gttaccgctg cagttgaaga     3720

actgaaagcg ctgtccgtac catgctctga ctctaaagcg attgctcagg ttggtaccat     3780

ctccgctaac tccgacgaaa ccgtaggtaa actgatcgct gaagcgatgg acaaagtcgg     3840

taaagaaggc gttatcaccg ttgaagacgg taccggtctg caggacgaac tggacgtggt     3900

tgaaggtatg cagttcgacc gtggctacct gtctccttac ttcatcaaca agccggaaac     3960

tggcgcagta gaactggaaa gcccgttcat cctgctggct gacaagaaaa tctccaacat     4020

ccgcgaaatg ctgccggttc tggaagctgt tgccaaagca ggcaaaccgc tgctgatcat     4080

cgctgaagat gtagaaggcg aagcgctggc aactctggtt gttaacacca tgcgtggcat     4140

cgtgaaagtc gctgcggtta aagcaccggg cttcggcgat cgtcgtaaag ctatgctgca     4200

ggatatcgca accctgactg gcggtaccgt gatctctgaa gagatcggta tggagctgga     4260

aaaagcaacc ctggaagacc tgggtcaggc taaacgtgtt gtgatcaaca aagacaccac     4320

cactatcatc gatggcgtgg gtgaagaagc tgcaatccag ggccgtgttg ctcagatccg     4380

tcagcagatt gaagaagcaa cttctgacta cgaccgtgaa aaactgcagg aacgcgtagc     4440

gaaactggca ggcggcgttg cagttatcaa agtgggtgct gctaccgaag ttgaaatgaa     4500

agagaaaaaa gcacgcgttg aagatgccct gcacgcgacc cgtgctgcgg tagaagaagg     4560

cgtggttgct ggtggtggtg ttgcgctgat ccgcgtagcg tctaaactgg ctgacctgcg     4620

tggtcagaac gaagaccaga acgtgggtat caaagttgca ctgcgtgcaa tggaagctcc     4680

gctgcgtcag atcgtattga actgcggcga agaaccgtct gttgttgcta acaccgttaa     4740

aggcggcgac ggcaactacg gttacaacgc agcaaccgaa gaatacggca acatgatcga     4800

catgggtatc ctggatccaa ccaaagtaac tcgttctgct ctgcagtacg cagcttctgt     4860

ggctggcctg atgatcacca ccgaatgcat ggttaccgac ctgccgaaaa acgatgcagc     4920

tgacttaggc gctgctggcg gtatgggcgg catgggtggc atgggcggca tgatgtaacc     4980

ccctagcata accccttggg gcctctaaac gggtcttgag gggttttttg cccctgagac     5040

gcgtcaatcg agttcgtacc taagggcgac accccctaat tagcccgggc gaaaggccca     5100

gtctttcgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcatggg     5160

gagtccccac actaccatcg gcgctacggc gtttcacttc tgagttcggc atggggtcag     5220

gtgggaccac cgcgctactg ccgccaggca aacaaggggt gttatgagcc atattcaggt     5280

ataaatgggc tcgcgataat gttcagaatt ggttaattgg ttgtaacact gacccctatt     5340

tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa     5400

atgcttcaat aatattgaaa aaggaagaat atgagccata ttcaacggga aacgtcgagg     5460

ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc tcgcgataat     5520

gtcgggcaat caggtgcgac aatctatcgc ttgtatggga agcccgatgc gccagagttg     5580

tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat ggtcagacta     5640

aactggctga cggaatttat gccacttccg accatcaagc attttatccg tactcctgat     5700

gatgcatggt tactcaccac tgcgatcccc ggaaaaacag cgttccaggt attagaagaa     5760

tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg ccggttgcac     5820

tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgcct cgctcaggcg     5880

caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga gcgtaatggc     5940

tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc accggattca     6000

gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg gaaattaata     6060

ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct tgccatccta     6120

tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca aaaatatggt     6180

attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga gtttttctaa     6240

gcggcgcgcc atcgaatggc gcaaaacctt tcgcggtatg gcatgatagc gcccggaaga     6300

gagtcaattc agggtggtga atatgaaacc agtaacgtta tacgatgtcg cagagtatgc     6360

cggtgtctct tatcagaccg tttcccgcgt ggtgaaccag gccagccacg tttctgcgaa     6420

aacgcgggaa aaagtggaag cggcgatggc ggagctgaat tacattccca accgcgtggc     6480

acaacaactg gcgggcaaac agtcgttgct gattggcgtt gccacctcca gtctggccct     6540

gcacgcgccg tcgcaaattg tcgcggcgat taaatctcgc gccgatcaac tgggtgccag     6600

cgtggtggtg tcgatggtag aacgaagcgg cgtcgaagcc tgtaaagcgg cggtgcacaa     6660

tcttctcgcg caacgcgtca gtgggctgat cattaactat ccgctggatg accaggatgc     6720

cattgctgtg gaagctgcct gcactaatgt tccggcgtta tttcttgatg tctctgacca     6780

gacacccatc aacagtatta ttttctccca tgaggacggt acgcgactgg gcgtggagca     6840

tctggtcgca ttgggtcacc agcaaatcgc gctgttagcg ggcccattaa gttctgtctc     6900

ggcgcgtctg cgtctggctg gctggcataa atatctcact cgcaatcaaa ttcagccgat     6960

agcggaacgg gaaggcgact ggagtgccat gtccggtttt caacaaacca tgcaaatgct     7020

gaatgagggc atcgttccca ctgcgatgct ggttgccaac gatcagatgg cgctgggcgc     7080

aatgcgcgcc attaccgagt ccgggctgcg cgttggtgcg gatatctcgg tagtgggata     7140

cgacgatacc gaagatagct catgttatat cccgccgtta accaccatca aacaggattt     7200

tcgcctgctg gggcaaacca gcgtggaccg cttgctgcaa ctctctcagg gccaggcggt     7260

gaagggcaat cagctgttgc cagtctcact ggtgaaaaga aaaaccaccc tggcgcccaa     7320

tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt     7380

ttcccgactg gaaagcgggc agtga                                           7405


