                               SEQUENCE LISTING

<110> ENZYVANT THERAPEUTICS GMBH
       
<120> FARBER DISEASE MARKERS AND USES THEREOF

<130> ENZ-004WO

<140>
<141>

<150> 62/648,775
<151> 2018-03-27

<160> 4     

<170> PatentIn version 3.5

<210> 1
<211> 395
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polypeptide

<220>
<223> Recombinant human acid ceramidase

<400> 1
Met Pro Gly Arg Ser Cys Val Ala Leu Val Leu Leu Ala Ala Ala Val 
1               5                   10                  15      


Ser Cys Ala Val Ala Gln His Ala Pro Pro Trp Thr Glu Asp Cys Arg 
            20                  25                  30          


Lys Ser Thr Tyr Pro Pro Ser Gly Pro Thr Tyr Arg Gly Ala Val Pro 
        35                  40                  45              


Trp Tyr Thr Ile Asn Leu Asp Leu Pro Pro Tyr Lys Arg Trp His Glu 
    50                  55                  60                  


Leu Met Leu Asp Lys Ala Pro Val Leu Lys Val Ile Val Asn Ser Leu 
65                  70                  75                  80  


Lys Asn Met Ile Asn Thr Phe Val Pro Ser Gly Lys Ile Met Gln Val 
                85                  90                  95      


Val Asp Glu Lys Leu Pro Gly Leu Leu Gly Asn Phe Pro Gly Pro Phe 
            100                 105                 110         


Glu Glu Glu Met Lys Gly Ile Ala Ala Val Thr Asp Ile Pro Leu Gly 
        115                 120                 125             


Glu Ile Ile Ser Phe Asn Ile Phe Tyr Glu Leu Phe Thr Ile Cys Thr 
    130                 135                 140                 


Ser Ile Val Ala Glu Asp Lys Lys Gly His Leu Ile His Gly Arg Asn 
145                 150                 155                 160 


Met Asp Phe Gly Val Phe Leu Gly Trp Asn Ile Asn Asn Asp Thr Trp 
                165                 170                 175     


Val Ile Thr Glu Gln Leu Lys Pro Leu Thr Val Asn Leu Asp Phe Gln 
            180                 185                 190         


Arg Asn Asn Lys Thr Val Phe Lys Ala Ser Ser Phe Ala Gly Tyr Val 
        195                 200                 205             


Gly Met Leu Thr Gly Phe Lys Pro Gly Leu Phe Ser Leu Thr Leu Asn 
    210                 215                 220                 


Glu Arg Phe Ser Ile Asn Gly Gly Tyr Leu Gly Ile Leu Glu Trp Ile 
225                 230                 235                 240 


Leu Gly Lys Lys Asp Val Met Trp Ile Gly Phe Leu Thr Arg Thr Val 
                245                 250                 255     


Leu Glu Asn Ser Thr Ser Tyr Glu Glu Ala Lys Asn Leu Leu Thr Lys 
            260                 265                 270         


Thr Lys Ile Leu Ala Pro Ala Tyr Phe Ile Leu Gly Gly Asn Gln Ser 
        275                 280                 285             


Gly Glu Gly Cys Val Ile Thr Arg Asp Arg Lys Glu Ser Leu Asp Val 
    290                 295                 300                 


Tyr Glu Leu Asp Ala Lys Gln Gly Arg Trp Tyr Val Val Gln Thr Asn 
305                 310                 315                 320 


Tyr Asp Arg Trp Lys His Pro Phe Phe Leu Asp Asp Arg Arg Thr Pro 
                325                 330                 335     


Ala Lys Met Cys Leu Asn Arg Thr Ser Gln Glu Asn Ile Ser Phe Glu 
            340                 345                 350         


Thr Met Tyr Asp Val Leu Ser Thr Lys Pro Val Leu Asn Lys Leu Thr 
        355                 360                 365             


Val Tyr Thr Thr Leu Ile Asp Val Thr Lys Gly Gln Phe Glu Thr Tyr 
    370                 375                 380                 


Leu Arg Asp Cys Pro Asp Pro Cys Ile Gly Trp 
385                 390                 395 


<210> 2
<211> 2618
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Encodes recombinant human acid ceramidase

<400> 2
ggctcggtcc gactattgcc cgcggtgggg gagggggatg gatcacgcca cgcgccaaag       60

gcgatcgcga ctctccttct gcaggtagcc tggaaggctc tctctctttc tctacgccac      120

ccttttcgtg gcactgaaaa gccccgtcct ctcctcccag tcccgcctcc tccgagcgtt      180

ccccctactg cctggaatgg tgcggtccca ggtcgcgggt cacgcggcgg agggggcgtg      240

gcctgccccc ggcccagccg gctcttcttt gcctctgctg gagtccgggg agtggcgttg      300

gctgctagag cgatgccggg ccggagttgc gtcgccttag tcctcctggc tgccgccgtc      360

agctgtgccg tcgcgcagca cgcgccgccg tggacagagg actgcagaaa atcaacctat      420

cctccttcag gaccaacgta cagaggtgca gttccatggt acaccataaa tcttgactta      480

ccaccctaca aaagatggca tgaattgatg cttgacaagg caccagtgct aaaggttata      540

gtgaattctc tgaagaatat gataaataca ttcgtgccaa gtggaaaaat tatgcaggtg      600

gtggatgaaa aattgcctgg cctacttggc aactttcctg gcccttttga agaggaaatg      660

aagggtattg ccgctgttac tgatatacct ttaggagaga ttatttcatt caatattttt      720

tatgaattat ttaccatttg tacttcaata gtagcagaag acaaaaaagg tcatctaata      780

catgggagaa acatggattt tggagtattt cttgggtgga acataaataa tgatacctgg      840

gtcataactg agcaactaaa acctttaaca gtgaatttgg atttccaaag aaacaacaaa      900

actgtcttca aggcttcaag ctttgctggc tatgtgggca tgttaacagg attcaaacca      960

ggactgttca gtcttacact gaatgaacgt ttcagtataa atggtggtta tctgggtatt     1020

ctagaatgga ttctgggaaa gaaagatgtc atgtggatag ggttcctcac tagaacagtt     1080

ctggaaaata gcacaagtta tgaagaagcc aagaatttat tgaccaagac caagatattg     1140

gccccagcct actttatcct gggaggcaac cagtctgggg aaggttgtgt gattacacga     1200

gacagaaagg aatcattgga tgtatatgaa ctcgatgcta agcagggtag atggtatgtg     1260

gtacaaacaa attatgaccg ttggaaacat cccttcttcc ttgatgatcg cagaacgcct     1320

gcaaagatgt gtctgaaccg caccagccaa gagaatatct catttgaaac catgtatgat     1380

gtcctgtcaa caaaacctgt cctcaacaag ctgaccgtat acacaacctt gatagatgtt     1440

accaaaggtc aattcgaaac ttacctgcgg gactgccctg acccttgtat aggttggtga     1500

gcacacgtct ggcctacaga atgcggcctc tgagacatga agacaccatc tccatgtgac     1560

cgaacactgc agctgtctga ccttccaaag actaagactc gcggcaggtt ctctttgagt     1620

caatagcttg tcttcgtcca tctgttgaca aatgacagat cttttttttt tccccctatc     1680

agttgatttt tcttatttac agataacttc tttaggggaa gtaaaacagt catctagaat     1740

tcactgagtt ttgtttcact ttgacatttg gggatctggt gggcagtcga accatggtga     1800

actccacctc cgtggaataa atggagattc agcgtgggtg ttgaatccag cacgtctgtg     1860

tgagtaacgg gacagtaaac actccacatt cttcagtttt tcacttctac ctacatattt     1920

gtatgttttt ctgtataaca gccttttcct tctggttcta actgctgtta aaattaatat     1980

atcattatct ttgctgttat tgacagcgat ataattttat tacatatgat tagagggatg     2040

agacagacat tcacctgtat atttctttta atgggcacaa aatgggccct tgcctctaaa     2100

tagcactttt tggggttcaa gaagtaatca gtatgcaaag caatctttta tacaataatt     2160

gaagtgttcc ctttttcata attactctac ttcccagtaa ccctaaggaa gttgctaact     2220

taaaaaactg catcccacgt tctgttaatt tagtaaataa acaagtcaaa gacttgtgga     2280

aaataggaag tgaacccata ttttaaattc tcataagtag cattcatgta ataaacaggt     2340

ttttagtttg ttcttcagat tgatagggag ttttaaagaa attttagtag ttactaaaat     2400

tatgttactg tatttttcag aaatcaaact gcttatgaaa agtactaata gaacttgtta     2460

acctttctaa ccttcacgat taactgtgaa atgtacgtca tttgtgcaag accgtttgtc     2520

cacttcattt tgtataatca cagttgtgtt cctgacactc aataaacagt cactggaaag     2580

agtgccagtc agcagtcatg cacgctgatt gggtgtgt                             2618


<210> 3
<211> 1276
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Encodes recombinant human acid ceramidase

<400> 3
aagcttaccg ccaccatgaa ctgctgcatc ggcctgggtg agaaggcgcg tggctcgcac       60

cgcgccagct acccctccct gagcgccctc ttcaccgagg cgtccatcct cggattcggg      120

agcttcgccg tcaaggcaca gtggaccgag gattgccgca agagtacgta cccccccagt      180

ggcccgacgt accgcggcgc cgtcccctgg tacacgatca acctggacct ccccccgtac      240

aagcgctggc acgagttgat gctggacaag gcccccgtac tgaaggtcat cgtgaactcc      300

ctgaagaaca tgatcaacac cttcgtcccc tcgggcaaga tcatgcaggt cgtggacgag      360

aagctgcccg ggctcctcgg caacttcccc ggcccgttcg aagaggagat gaagggcatc      420

gcggccgtca ctgacatccc cctgggcgag atcatcagct tcaacatctt ctacgagctg      480

ttcaccatct gcacctccat cgtagccgag gacaagaagg gccacctgat ccacggtcgc      540

aacatggact tcggcgtctt cctgggctgg aacatcaaca acgacacctg ggtcatcacc      600

gagcagctga agccgctcac cgtgaacctc gatttccagc gcaacaacaa gacggtgttc      660

aaggccagct ccttcgccgg gtacgtcggg atgctcacgg gcttcaagcc gggactgttc      720

tcgctgaccc tcaacgagcg gttctccatc aacgggggct acctcggcat cctggagtgg      780

attctcggca agaaggacgt gatgtggatc ggcttcctca cacggaccgt gctggaaaac      840

tccactagtt acgaggaggc caagaacctg ctgaccaaga cgaagatcct ggccccggca      900

tacttcatcc tgggcggcaa ccagtcgggc gaggggtgcg tcatcacccg cgaccggaag      960

gagtccctgg acgtctatga gctggacgcc aagcagggcc gctggtacgt cgtccagacg     1020

aactacgacc gatggaagca ccccttcttc ctcgacgacc ggcgcacgcc cgccaagatg     1080

tgcctgaacc gcaccagcca ggagaacatc tcgttcgaga cgatgtacga cgtgctgtcg     1140

accaagcccg tgctcaacaa gctgacggtc tacaccacgc tgatcgacgt gacgaaaggc     1200

cagttcgaaa cgtacctgcg ggactgcccg gacccttgca tcggctggtg ataatctaga     1260

gtcggggcgg ccggcc                                                     1276


<210> 4
<211> 1276
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      polynucleotide

<220>
<223> Encodes recombinant human acid ceramidase

<400> 4
aagcttaccg ccaccatgaa ctgctgcatc gggctgggag agaaagctcg cgggtcccac       60

cgggcctcct acccaagtct cagcgcgctt ttcaccgagg cctcaattct gggatttggc      120

agctttgctg tgaaagccca atggacagag gactgcagaa aatcaaccta tcctccttca      180

ggaccaacgt acagaggtgc agttccatgg tacaccataa atcttgactt accaccctac      240

aaaagatggc atgaattgat gcttgacaag gcaccagtgc taaaggttat agtgaattct      300

ctgaagaata tgataaatac attcgtgcca agtggaaaaa ttatgcaggt ggtggatgaa      360

aaattgcctg gcctacttgg caactttcct ggcccttttg aagaggaaat gaagggtatt      420

gccgctgtta ctgatatacc tttaggagag attatttcat tcaatatttt ttatgaatta      480

tttaccattt gtacttcaat agtagcagaa gacaaaaaag gtcatctaat acatgggaga      540

aacatggatt ttggagtatt tcttgggtgg aacataaata atgatacctg ggtcataact      600

gagcaactaa aacctttaac agtgaatttg gatttccaaa gaaacaacaa aactgtcttc      660

aaggcttcca gctttgctgg ctatgtgggc atgttaacag gattcaaacc aggactgttc      720

agtcttacac tgaatgaacg tttcagtata aatggtggtt atctgggtat tctagaatgg      780

attctgggaa agaaagatgt catgtggata gggttcctca ctagaacagt tctggaaaat      840

agcacaagtt atgaagaagc caagaattta ttgaccaaga ccaagatatt ggccccagcc      900

tactttatcc tgggaggcaa ccagtctggg gaaggttgtg tgattacacg agacagaaag      960

gaatcattgg atgtatatga actcgatgct aagcagggta gatggtatgt ggtacaaaca     1020

aattatgacc gttggaaaca tcccttcttc cttgatgatc gcagaacgcc tgcaaagatg     1080

tgtctgaacc gcaccagcca agagaatatc tcatttgaaa ccatgtatga tgtcctgtca     1140

acaaaacctg tcctcaacaa gctgaccgta tacacaacct tgatagatgt taccaaaggt     1200

caattcgaaa cttacctgcg ggactgccct gacccttgta taggttggtg ataacctagg     1260

gtcggggcgg ccggcc                                                     1276


