                         SEQUENCE LISTING

<110>  LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC
 
<120>  CHIMERIC AMYLASES COMPRISING AN HETEROLOGOUS STARCH BINDING 
       DOMAIN

<130>  55729550-26PCT

<150>  62/648,243
<151>  2013-03-26

<160>  24    

<170>  PatentIn version 3.5

<210>  1
<211>  1956
<212>  DNA
<213>  Bacillus amyloliquefaciens

<400>  1
atgttgttgc aagccttctt gtttttgttg gctggttttg ctgctaagat ttctgctggt       60

ccagctgctg ctaatgctga aactgctaac aaatctaaca aggttactgc ctcctctgtt      120

aagaatggta ctattttaca tgcctggaac tggtctttca acactttgac tcaaaacatg      180

aaggacatta gagatgctgg ttacgctgct attcaaacct ctccaatcaa tcaagtcaaa      240

gaaggtaatc aaggtgacaa gtctatgaga aattggtact ggttgtacca acctacctct      300

taccaaatcg gtaatagata cttgggtact gaacaagaat tcaaggatat gtgtgctgct      360

gctgaaaagt atggtgttaa ggttatagtt gacgccgtta ttaaccatac cacatctgat      420

tatggtgcca tctctgacga aatcaagaga attccaaatt ggactcatgg taacacccaa      480

atcaagaatt ggtctgatag atgggatgtc acccaaaatt ctttgttggg tttgtacgat      540

tggaataccc aaaacaccga agttcaagtc tacttgaaga gattcttgga aagagctttg      600

aacgatggtg ctgatggttt tagatatgat gccgccaaac atatcgaatt gccagatgat      660

ggtaattacg gttctcaatt ctggccaaat attaccaaca cttccgctga atttcaatac      720

ggtgaaatat tgcaagactc cgcttctaga gatactgctt atgctaatta catgaacgtt      780

accgcttcta actacggtca ttctattaga tctgccttga agaacagaaa cttgtccgtt      840

tccaacattt ctcattacgc ctctgatgtt tctgccgata agttggttac ttgggttgaa      900

tctcatgata cctacgctaa cgatgatgaa gaatctactt ggatgtccga tgatgatatt      960

agattgggtt gggctgttat cggttctaga tctggttcta ctcctttgtt tttctcaaga     1020

cctgaaggtg gtggtaatgg tgttagattc ccaggtaaat ctcaaattgg tgatagaggt     1080

tctgccttgt ttaaggatca agctattact gctgttaaca ccttccataa tgttatggct     1140

ggtcaaccag aagaattgtc taatccaaac ggtaacaatc aagttttcat gaatcaaaga     1200

ggttccaagg gtgttgtttt ggctaatgca ggttcttctt ccgttactat taacacctct     1260

gctaaattgc ctgatggtag atacgataat agagctggtg ctggttcttt tcaagttgct     1320

aatggtaaat tgaccggtac tatcaatgct agatctgctg ctgtcttgta cccagatgat     1380

attggtaatg ctccacacgt ctttttggaa aactatcaaa ctggtgccgt tcactctttc     1440

aacgatcaat tgactgttac cttgagagct aatgctaaga ctactaaggc cgtctaccaa     1500

atcaacaacg gtcaacaaac tgctttcaaa gatggtgaca gattgaccat tggtaagggt     1560

gatcctattg gtactaccta caacattaag ttgactggta ctaatggtga aggtgctgct     1620

agaactcaag aatacacttt cgttaagaag gatccatccc aaactaacat catcggttac     1680

caaaatccag atcattgggg tcaagttaac gcctacatct acaaacatga tggtggtaga     1740

gctattgaat tgactggttc ttggccaggt aaagctatga ctaagaatgc taacggtatg     1800

tacacattga ccttgccaga aaacactgat acagctaacg ctaaggttat ctttaacaac     1860

ggttctgctc aagtcccagg tcaaaatcaa ccaggttttg attacgttca aaacggtttg     1920

tacaacaact ctggtttgaa cggttatttg ccacac                               1956


<210>  2
<211>  652
<212>  PRT
<213>  Bacillus amyloliquefaciens

<400>  2

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala Gly Pro Ala Ala Ala Asn Ala Glu Thr Ala Asn Lys Ser 
            20                  25                  30          


Asn Lys Val Thr Ala Ser Ser Val Lys Asn Gly Thr Ile Leu His Ala 
        35                  40                  45              


Trp Asn Trp Ser Phe Asn Thr Leu Thr Gln Asn Met Lys Asp Ile Arg 
    50                  55                  60                  


Asp Ala Gly Tyr Ala Ala Ile Gln Thr Ser Pro Ile Asn Gln Val Lys 
65                  70                  75                  80  


Glu Gly Asn Gln Gly Asp Lys Ser Met Arg Asn Trp Tyr Trp Leu Tyr 
                85                  90                  95      


Gln Pro Thr Ser Tyr Gln Ile Gly Asn Arg Tyr Leu Gly Thr Glu Gln 
            100                 105                 110         


Glu Phe Lys Asp Met Cys Ala Ala Ala Glu Lys Tyr Gly Val Lys Val 
        115                 120                 125             


Ile Val Asp Ala Val Ile Asn His Thr Thr Ser Asp Tyr Gly Ala Ile 
    130                 135                 140                 


Ser Asp Glu Ile Lys Arg Ile Pro Asn Trp Thr His Gly Asn Thr Gln 
145                 150                 155                 160 


Ile Lys Asn Trp Ser Asp Arg Trp Asp Val Thr Gln Asn Ser Leu Leu 
                165                 170                 175     


Gly Leu Tyr Asp Trp Asn Thr Gln Asn Thr Glu Val Gln Val Tyr Leu 
            180                 185                 190         


Lys Arg Phe Leu Glu Arg Ala Leu Asn Asp Gly Ala Asp Gly Phe Arg 
        195                 200                 205             


Tyr Asp Ala Ala Lys His Ile Glu Leu Pro Asp Asp Gly Asn Tyr Gly 
    210                 215                 220                 


Ser Gln Phe Trp Pro Asn Ile Thr Asn Thr Ser Ala Glu Phe Gln Tyr 
225                 230                 235                 240 


Gly Glu Ile Leu Gln Asp Ser Ala Ser Arg Asp Thr Ala Tyr Ala Asn 
                245                 250                 255     


Tyr Met Asn Val Thr Ala Ser Asn Tyr Gly His Ser Ile Arg Ser Ala 
            260                 265                 270         


Leu Lys Asn Arg Asn Leu Ser Val Ser Asn Ile Ser His Tyr Ala Ser 
        275                 280                 285             


Asp Val Ser Ala Asp Lys Leu Val Thr Trp Val Glu Ser His Asp Thr 
    290                 295                 300                 


Tyr Ala Asn Asp Asp Glu Glu Ser Thr Trp Met Ser Asp Asp Asp Ile 
305                 310                 315                 320 


Arg Leu Gly Trp Ala Val Ile Gly Ser Arg Ser Gly Ser Thr Pro Leu 
                325                 330                 335     


Phe Phe Ser Arg Pro Glu Gly Gly Gly Asn Gly Val Arg Phe Pro Gly 
            340                 345                 350         


Lys Ser Gln Ile Gly Asp Arg Gly Ser Ala Leu Phe Lys Asp Gln Ala 
        355                 360                 365             


Ile Thr Ala Val Asn Thr Phe His Asn Val Met Ala Gly Gln Pro Glu 
    370                 375                 380                 


Glu Leu Ser Asn Pro Asn Gly Asn Asn Gln Val Phe Met Asn Gln Arg 
385                 390                 395                 400 


Gly Ser Lys Gly Val Val Leu Ala Asn Ala Gly Ser Ser Ser Val Thr 
                405                 410                 415     


Ile Asn Thr Ser Ala Lys Leu Pro Asp Gly Arg Tyr Asp Asn Arg Ala 
            420                 425                 430         


Gly Ala Gly Ser Phe Gln Val Ala Asn Gly Lys Leu Thr Gly Thr Ile 
        435                 440                 445             


Asn Ala Arg Ser Ala Ala Val Leu Tyr Pro Asp Asp Ile Gly Asn Ala 
    450                 455                 460                 


Pro His Val Phe Leu Glu Asn Tyr Gln Thr Gly Ala Val His Ser Phe 
465                 470                 475                 480 


Asn Asp Gln Leu Thr Val Thr Leu Arg Ala Asn Ala Lys Thr Thr Lys 
                485                 490                 495     


Ala Val Tyr Gln Ile Asn Asn Gly Gln Gln Thr Ala Phe Lys Asp Gly 
            500                 505                 510         


Asp Arg Leu Thr Ile Gly Lys Gly Asp Pro Ile Gly Thr Thr Tyr Asn 
        515                 520                 525             


Ile Lys Leu Thr Gly Thr Asn Gly Glu Gly Ala Ala Arg Thr Gln Glu 
    530                 535                 540                 


Tyr Thr Phe Val Lys Lys Asp Pro Ser Gln Thr Asn Ile Ile Gly Tyr 
545                 550                 555                 560 


Gln Asn Pro Asp His Trp Gly Gln Val Asn Ala Tyr Ile Tyr Lys His 
                565                 570                 575     


Asp Gly Gly Arg Ala Ile Glu Leu Thr Gly Ser Trp Pro Gly Lys Ala 
            580                 585                 590         


Met Thr Lys Asn Ala Asn Gly Met Tyr Thr Leu Thr Leu Pro Glu Asn 
        595                 600                 605             


Thr Asp Thr Ala Asn Ala Lys Val Ile Phe Asn Asn Gly Ser Ala Gln 
    610                 615                 620                 


Val Pro Gly Gln Asn Gln Pro Gly Phe Asp Tyr Val Gln Asn Gly Leu 
625                 630                 635                 640 


Tyr Asn Asn Ser Gly Leu Asn Gly Tyr Leu Pro His 
                645                 650         


<210>  3
<211>  633
<212>  PRT
<213>  Bacillus amyloliquefaciens

<400>  3

Gly Pro Ala Ala Ala Asn Ala Glu Thr Ala Asn Lys Ser Asn Lys Val 
1               5                   10                  15      


Thr Ala Ser Ser Val Lys Asn Gly Thr Ile Leu His Ala Trp Asn Trp 
            20                  25                  30          


Ser Phe Asn Thr Leu Thr Gln Asn Met Lys Asp Ile Arg Asp Ala Gly 
        35                  40                  45              


Tyr Ala Ala Ile Gln Thr Ser Pro Ile Asn Gln Val Lys Glu Gly Asn 
    50                  55                  60                  


Gln Gly Asp Lys Ser Met Arg Asn Trp Tyr Trp Leu Tyr Gln Pro Thr 
65                  70                  75                  80  


Ser Tyr Gln Ile Gly Asn Arg Tyr Leu Gly Thr Glu Gln Glu Phe Lys 
                85                  90                  95      


Asp Met Cys Ala Ala Ala Glu Lys Tyr Gly Val Lys Val Ile Val Asp 
            100                 105                 110         


Ala Val Ile Asn His Thr Thr Ser Asp Tyr Gly Ala Ile Ser Asp Glu 
        115                 120                 125             


Ile Lys Arg Ile Pro Asn Trp Thr His Gly Asn Thr Gln Ile Lys Asn 
    130                 135                 140                 


Trp Ser Asp Arg Trp Asp Val Thr Gln Asn Ser Leu Leu Gly Leu Tyr 
145                 150                 155                 160 


Asp Trp Asn Thr Gln Asn Thr Glu Val Gln Val Tyr Leu Lys Arg Phe 
                165                 170                 175     


Leu Glu Arg Ala Leu Asn Asp Gly Ala Asp Gly Phe Arg Tyr Asp Ala 
            180                 185                 190         


Ala Lys His Ile Glu Leu Pro Asp Asp Gly Asn Tyr Gly Ser Gln Phe 
        195                 200                 205             


Trp Pro Asn Ile Thr Asn Thr Ser Ala Glu Phe Gln Tyr Gly Glu Ile 
    210                 215                 220                 


Leu Gln Asp Ser Ala Ser Arg Asp Thr Ala Tyr Ala Asn Tyr Met Asn 
225                 230                 235                 240 


Val Thr Ala Ser Asn Tyr Gly His Ser Ile Arg Ser Ala Leu Lys Asn 
                245                 250                 255     


Arg Asn Leu Ser Val Ser Asn Ile Ser His Tyr Ala Ser Asp Val Ser 
            260                 265                 270         


Ala Asp Lys Leu Val Thr Trp Val Glu Ser His Asp Thr Tyr Ala Asn 
        275                 280                 285             


Asp Asp Glu Glu Ser Thr Trp Met Ser Asp Asp Asp Ile Arg Leu Gly 
    290                 295                 300                 


Trp Ala Val Ile Gly Ser Arg Ser Gly Ser Thr Pro Leu Phe Phe Ser 
305                 310                 315                 320 


Arg Pro Glu Gly Gly Gly Asn Gly Val Arg Phe Pro Gly Lys Ser Gln 
                325                 330                 335     


Ile Gly Asp Arg Gly Ser Ala Leu Phe Lys Asp Gln Ala Ile Thr Ala 
            340                 345                 350         


Val Asn Thr Phe His Asn Val Met Ala Gly Gln Pro Glu Glu Leu Ser 
        355                 360                 365             


Asn Pro Asn Gly Asn Asn Gln Val Phe Met Asn Gln Arg Gly Ser Lys 
    370                 375                 380                 


Gly Val Val Leu Ala Asn Ala Gly Ser Ser Ser Val Thr Ile Asn Thr 
385                 390                 395                 400 


Ser Ala Lys Leu Pro Asp Gly Arg Tyr Asp Asn Arg Ala Gly Ala Gly 
                405                 410                 415     


Ser Phe Gln Val Ala Asn Gly Lys Leu Thr Gly Thr Ile Asn Ala Arg 
            420                 425                 430         


Ser Ala Ala Val Leu Tyr Pro Asp Asp Ile Gly Asn Ala Pro His Val 
        435                 440                 445             


Phe Leu Glu Asn Tyr Gln Thr Gly Ala Val His Ser Phe Asn Asp Gln 
    450                 455                 460                 


Leu Thr Val Thr Leu Arg Ala Asn Ala Lys Thr Thr Lys Ala Val Tyr 
465                 470                 475                 480 


Gln Ile Asn Asn Gly Gln Gln Thr Ala Phe Lys Asp Gly Asp Arg Leu 
                485                 490                 495     


Thr Ile Gly Lys Gly Asp Pro Ile Gly Thr Thr Tyr Asn Ile Lys Leu 
            500                 505                 510         


Thr Gly Thr Asn Gly Glu Gly Ala Ala Arg Thr Gln Glu Tyr Thr Phe 
        515                 520                 525             


Val Lys Lys Asp Pro Ser Gln Thr Asn Ile Ile Gly Tyr Gln Asn Pro 
    530                 535                 540                 


Asp His Trp Gly Gln Val Asn Ala Tyr Ile Tyr Lys His Asp Gly Gly 
545                 550                 555                 560 


Arg Ala Ile Glu Leu Thr Gly Ser Trp Pro Gly Lys Ala Met Thr Lys 
                565                 570                 575     


Asn Ala Asn Gly Met Tyr Thr Leu Thr Leu Pro Glu Asn Thr Asp Thr 
            580                 585                 590         


Ala Asn Ala Lys Val Ile Phe Asn Asn Gly Ser Ala Gln Val Pro Gly 
        595                 600                 605             


Gln Asn Gln Pro Gly Phe Asp Tyr Val Gln Asn Gly Leu Tyr Asn Asn 
    610                 615                 620                 


Ser Gly Leu Asn Gly Tyr Leu Pro His 
625                 630             


<210>  4
<211>  2394
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Chimeric sequence

<400>  4
atgttgttgc aagccttctt gtttttgttg gctggttttg ctgctaagat ttctgctggt       60

ccagctgctg ctaatgctga aactgctaac aaatctaaca aggttactgc ctcctctgtt      120

aagaatggta ctattttaca tgcctggaac tggtctttca acactttgac tcaaaacatg      180

aaggacatta gagatgctgg ttacgctgct attcaaacct ctccaatcaa tcaagtcaaa      240

gaaggtaatc aaggcgacaa gtctatgaga aattggtatt ggttgtacca gcctacctct      300

taccaaatcg gtaatagata tttgggtact gagcaagagt tcaaggatat gtgtgctgct      360

gctgaaaagt atggtgttaa ggttatagtt gacgccgtta ttaaccatac cacatctgat      420

tatggtgcca tctctgacga aatcaagaga attccaaatt ggactcatgg taacacccaa      480

atcaagaatt ggtctgatag atgggatgtg acccaaaatt ctttgttggg tctgtacgat      540

tggaataccc aaaacactga agttcaggtc tacttgaaga gatttttgga aagggctttg      600

aacgatggtg ctgatggttt tagatatgat gccgccaaac atatcgaatt gccagatgat      660

ggtaattacg gttctcaatt ctggcccaat attaccaata cttccgctga atttcagtac      720

ggtgaaatct tacaagactc cgcttctaga gatactgctt atgctaatta catgaacgtt      780

accgcttcta actacggtca ttctattaga tctgccctga agaacagaaa cttgtccgtt      840

tctaacattt cccattacgc ctctgatgtt tctgctgata agttggttac ttgggttgaa      900

tctcatgata cctacgctaa cgatgatgaa gaatctactt ggatgtccga tgacgatatt      960

agattaggtt gggctgttat cggttctaga tctggttcta ctcctttgtt tttctcaaga     1020

cctgaaggtg gtggtaatgg tgttagattc ccaggtaaat ctcaaattgg tgatagaggt     1080

tctgctttgt ttaaggatca agctattact gccgttaaca cctttcataa tgttatggct     1140

ggtcagccag aagaattgtc taatccaaat ggtaacaacc aggttttcat gaatcaaagg     1200

ggttctaagg gtgttgtttt ggctaatgca ggttcttctt ccgttactat taacacctct     1260

gctaaattgc ctgatggtag atacgataat agagctggtg ctggttcttt tcaagttgct     1320

aatggtaaat tgaccggtac tatcaatgct agatctgctg ctgtcttgta cccagatgat     1380

attggtaacg ctccacatgt tttcttggaa aactatcaaa ctggtgccgt tcactctttc     1440

aacgatcaat tgactgttac cttgagagct aatgctaaga ctactaaggc cgtttaccag     1500

attaacaacg gtcaacaaac tgctttcaaa gacggtgata gattgactat tggtaagggt     1560

gatccaattg gtactaccta caacattaag ttgactggca ctaatggtga aggtgctgct     1620

agaactcaag agtacacttt tgttaagaag gatccatctc agaccaacat catcggttat     1680

caaaatccag atcattgggg tcaagttaac gcctacatct acaaacatga tggtggtaga     1740

gctattgaat tgactggttc ttggccaggt aaagctatga ctaagaatgc taacggtatg     1800

tacacattga ccttgccaga aaacacagat acagctaacg ctaaggttat cttcaacaat     1860

ggttctgctc aagtcccagg tcaaaatcaa cctggttttg attatgttca gaacggcttg     1920

tacaacaact ctggtttgaa tggttatttg ccacatactg gtggtactac tactacagct     1980

actccaacag gttctggttc tgttacttct acttctaaaa ctaccgctac tgcttctaag     2040

acttctacct ctacttcttc cacttcttgt actacaccaa ctgctgttgc tgttactttt     2100

gatttgactg ctactacaac ttacggcgag aacatctatt tggttggttc catttctcaa     2160

ctaggtgatt gggaaacttc tgatggtatt gctttgtctg cagataagta cacttcttct     2220

gatccattgt ggtacgttac tgttacattg ccagctggtg aatcttttga gtacaagttc     2280

atcagaatcg agtccgatga ttctgttgaa tgggaatctg atccaaatag agagtacaca     2340

gttcctcaag cttgtggtac atctactgct actgttactg atacttggag gtga           2394


<210>  5
<211>  797
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric sequence

<400>  5

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala Gly Pro Ala Ala Ala Asn Ala Glu Thr Ala Asn Lys Ser 
            20                  25                  30          


Asn Lys Val Thr Ala Ser Ser Val Lys Asn Gly Thr Ile Leu His Ala 
        35                  40                  45              


Trp Asn Trp Ser Phe Asn Thr Leu Thr Gln Asn Met Lys Asp Ile Arg 
    50                  55                  60                  


Asp Ala Gly Tyr Ala Ala Ile Gln Thr Ser Pro Ile Asn Gln Val Lys 
65                  70                  75                  80  


Glu Gly Asn Gln Gly Asp Lys Ser Met Arg Asn Trp Tyr Trp Leu Tyr 
                85                  90                  95      


Gln Pro Thr Ser Tyr Gln Ile Gly Asn Arg Tyr Leu Gly Thr Glu Gln 
            100                 105                 110         


Glu Phe Lys Asp Met Cys Ala Ala Ala Glu Lys Tyr Gly Val Lys Val 
        115                 120                 125             


Ile Val Asp Ala Val Ile Asn His Thr Thr Ser Asp Tyr Gly Ala Ile 
    130                 135                 140                 


Ser Asp Glu Ile Lys Arg Ile Pro Asn Trp Thr His Gly Asn Thr Gln 
145                 150                 155                 160 


Ile Lys Asn Trp Ser Asp Arg Trp Asp Val Thr Gln Asn Ser Leu Leu 
                165                 170                 175     


Gly Leu Tyr Asp Trp Asn Thr Gln Asn Thr Glu Val Gln Val Tyr Leu 
            180                 185                 190         


Lys Arg Phe Leu Glu Arg Ala Leu Asn Asp Gly Ala Asp Gly Phe Arg 
        195                 200                 205             


Tyr Asp Ala Ala Lys His Ile Glu Leu Pro Asp Asp Gly Asn Tyr Gly 
    210                 215                 220                 


Ser Gln Phe Trp Pro Asn Ile Thr Asn Thr Ser Ala Glu Phe Gln Tyr 
225                 230                 235                 240 


Gly Glu Ile Leu Gln Asp Ser Ala Ser Arg Asp Thr Ala Tyr Ala Asn 
                245                 250                 255     


Tyr Met Asn Val Thr Ala Ser Asn Tyr Gly His Ser Ile Arg Ser Ala 
            260                 265                 270         


Leu Lys Asn Arg Asn Leu Ser Val Ser Asn Ile Ser His Tyr Ala Ser 
        275                 280                 285             


Asp Val Ser Ala Asp Lys Leu Val Thr Trp Val Glu Ser His Asp Thr 
    290                 295                 300                 


Tyr Ala Asn Asp Asp Glu Glu Ser Thr Trp Met Ser Asp Asp Asp Ile 
305                 310                 315                 320 


Arg Leu Gly Trp Ala Val Ile Gly Ser Arg Ser Gly Ser Thr Pro Leu 
                325                 330                 335     


Phe Phe Ser Arg Pro Glu Gly Gly Gly Asn Gly Val Arg Phe Pro Gly 
            340                 345                 350         


Lys Ser Gln Ile Gly Asp Arg Gly Ser Ala Leu Phe Lys Asp Gln Ala 
        355                 360                 365             


Ile Thr Ala Val Asn Thr Phe His Asn Val Met Ala Gly Gln Pro Glu 
    370                 375                 380                 


Glu Leu Ser Asn Pro Asn Gly Asn Asn Gln Val Phe Met Asn Gln Arg 
385                 390                 395                 400 


Gly Ser Lys Gly Val Val Leu Ala Asn Ala Gly Ser Ser Ser Val Thr 
                405                 410                 415     


Ile Asn Thr Ser Ala Lys Leu Pro Asp Gly Arg Tyr Asp Asn Arg Ala 
            420                 425                 430         


Gly Ala Gly Ser Phe Gln Val Ala Asn Gly Lys Leu Thr Gly Thr Ile 
        435                 440                 445             


Asn Ala Arg Ser Ala Ala Val Leu Tyr Pro Asp Asp Ile Gly Asn Ala 
    450                 455                 460                 


Pro His Val Phe Leu Glu Asn Tyr Gln Thr Gly Ala Val His Ser Phe 
465                 470                 475                 480 


Asn Asp Gln Leu Thr Val Thr Leu Arg Ala Asn Ala Lys Thr Thr Lys 
                485                 490                 495     


Ala Val Tyr Gln Ile Asn Asn Gly Gln Gln Thr Ala Phe Lys Asp Gly 
            500                 505                 510         


Asp Arg Leu Thr Ile Gly Lys Gly Asp Pro Ile Gly Thr Thr Tyr Asn 
        515                 520                 525             


Ile Lys Leu Thr Gly Thr Asn Gly Glu Gly Ala Ala Arg Thr Gln Glu 
    530                 535                 540                 


Tyr Thr Phe Val Lys Lys Asp Pro Ser Gln Thr Asn Ile Ile Gly Tyr 
545                 550                 555                 560 


Gln Asn Pro Asp His Trp Gly Gln Val Asn Ala Tyr Ile Tyr Lys His 
                565                 570                 575     


Asp Gly Gly Arg Ala Ile Glu Leu Thr Gly Ser Trp Pro Gly Lys Ala 
            580                 585                 590         


Met Thr Lys Asn Ala Asn Gly Met Tyr Thr Leu Thr Leu Pro Glu Asn 
        595                 600                 605             


Thr Asp Thr Ala Asn Ala Lys Val Ile Phe Asn Asn Gly Ser Ala Gln 
    610                 615                 620                 


Val Pro Gly Gln Asn Gln Pro Gly Phe Asp Tyr Val Gln Asn Gly Leu 
625                 630                 635                 640 


Tyr Asn Asn Ser Gly Leu Asn Gly Tyr Leu Pro His Thr Gly Gly Thr 
                645                 650                 655     


Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val Thr Ser Thr Ser 
            660                 665                 670         


Lys Thr Thr Ala Thr Ala Ser Lys Thr Ser Thr Ser Thr Ser Ser Thr 
        675                 680                 685             


Ser Cys Thr Thr Pro Thr Ala Val Ala Val Thr Phe Asp Leu Thr Ala 
    690                 695                 700                 


Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln 
705                 710                 715                 720 


Leu Gly Asp Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys 
                725                 730                 735     


Tyr Thr Ser Ser Asp Pro Leu Trp Tyr Val Thr Val Thr Leu Pro Ala 
            740                 745                 750         


Gly Glu Ser Phe Glu Tyr Lys Phe Ile Arg Ile Glu Ser Asp Asp Ser 
        755                 760                 765             


Val Glu Trp Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala 
    770                 775                 780                 


Cys Gly Thr Ser Thr Ala Thr Val Thr Asp Thr Trp Arg 
785                 790                 795         


<210>  6
<211>  19
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Signal sequence

<400>  6

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala 
            


<210>  7
<211>  108
<212>  PRT
<213>  Aspergillus niger

<400>  7

Cys Thr Thr Pro Thr Ala Val Ala Val Thr Phe Asp Leu Thr Ala Thr 
1               5                   10                  15      


Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln Leu 
            20                  25                  30          


Gly Asp Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys Tyr 
        35                  40                  45              


Thr Ser Ser Asp Pro Leu Trp Tyr Val Thr Val Thr Leu Pro Ala Gly 
    50                  55                  60                  


Glu Ser Phe Glu Tyr Lys Phe Ile Arg Ile Glu Ser Asp Asp Ser Val 
65                  70                  75                  80  


Glu Trp Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala Cys 
                85                  90                  95      


Gly Thr Ser Thr Ala Thr Val Thr Asp Thr Trp Arg 
            100                 105             


<210>  8
<211>  778
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Chimeric sequence

<400>  8

Gly Pro Ala Ala Ala Asn Ala Glu Thr Ala Asn Lys Ser Asn Lys Val 
1               5                   10                  15      


Thr Ala Ser Ser Val Lys Asn Gly Thr Ile Leu His Ala Trp Asn Trp 
            20                  25                  30          


Ser Phe Asn Thr Leu Thr Gln Asn Met Lys Asp Ile Arg Asp Ala Gly 
        35                  40                  45              


Tyr Ala Ala Ile Gln Thr Ser Pro Ile Asn Gln Val Lys Glu Gly Asn 
    50                  55                  60                  


Gln Gly Asp Lys Ser Met Arg Asn Trp Tyr Trp Leu Tyr Gln Pro Thr 
65                  70                  75                  80  


Ser Tyr Gln Ile Gly Asn Arg Tyr Leu Gly Thr Glu Gln Glu Phe Lys 
                85                  90                  95      


Asp Met Cys Ala Ala Ala Glu Lys Tyr Gly Val Lys Val Ile Val Asp 
            100                 105                 110         


Ala Val Ile Asn His Thr Thr Ser Asp Tyr Gly Ala Ile Ser Asp Glu 
        115                 120                 125             


Ile Lys Arg Ile Pro Asn Trp Thr His Gly Asn Thr Gln Ile Lys Asn 
    130                 135                 140                 


Trp Ser Asp Arg Trp Asp Val Thr Gln Asn Ser Leu Leu Gly Leu Tyr 
145                 150                 155                 160 


Asp Trp Asn Thr Gln Asn Thr Glu Val Gln Val Tyr Leu Lys Arg Phe 
                165                 170                 175     


Leu Glu Arg Ala Leu Asn Asp Gly Ala Asp Gly Phe Arg Tyr Asp Ala 
            180                 185                 190         


Ala Lys His Ile Glu Leu Pro Asp Asp Gly Asn Tyr Gly Ser Gln Phe 
        195                 200                 205             


Trp Pro Asn Ile Thr Asn Thr Ser Ala Glu Phe Gln Tyr Gly Glu Ile 
    210                 215                 220                 


Leu Gln Asp Ser Ala Ser Arg Asp Thr Ala Tyr Ala Asn Tyr Met Asn 
225                 230                 235                 240 


Val Thr Ala Ser Asn Tyr Gly His Ser Ile Arg Ser Ala Leu Lys Asn 
                245                 250                 255     


Arg Asn Leu Ser Val Ser Asn Ile Ser His Tyr Ala Ser Asp Val Ser 
            260                 265                 270         


Ala Asp Lys Leu Val Thr Trp Val Glu Ser His Asp Thr Tyr Ala Asn 
        275                 280                 285             


Asp Asp Glu Glu Ser Thr Trp Met Ser Asp Asp Asp Ile Arg Leu Gly 
    290                 295                 300                 


Trp Ala Val Ile Gly Ser Arg Ser Gly Ser Thr Pro Leu Phe Phe Ser 
305                 310                 315                 320 


Arg Pro Glu Gly Gly Gly Asn Gly Val Arg Phe Pro Gly Lys Ser Gln 
                325                 330                 335     


Ile Gly Asp Arg Gly Ser Ala Leu Phe Lys Asp Gln Ala Ile Thr Ala 
            340                 345                 350         


Val Asn Thr Phe His Asn Val Met Ala Gly Gln Pro Glu Glu Leu Ser 
        355                 360                 365             


Asn Pro Asn Gly Asn Asn Gln Val Phe Met Asn Gln Arg Gly Ser Lys 
    370                 375                 380                 


Gly Val Val Leu Ala Asn Ala Gly Ser Ser Ser Val Thr Ile Asn Thr 
385                 390                 395                 400 


Ser Ala Lys Leu Pro Asp Gly Arg Tyr Asp Asn Arg Ala Gly Ala Gly 
                405                 410                 415     


Ser Phe Gln Val Ala Asn Gly Lys Leu Thr Gly Thr Ile Asn Ala Arg 
            420                 425                 430         


Ser Ala Ala Val Leu Tyr Pro Asp Asp Ile Gly Asn Ala Pro His Val 
        435                 440                 445             


Phe Leu Glu Asn Tyr Gln Thr Gly Ala Val His Ser Phe Asn Asp Gln 
    450                 455                 460                 


Leu Thr Val Thr Leu Arg Ala Asn Ala Lys Thr Thr Lys Ala Val Tyr 
465                 470                 475                 480 


Gln Ile Asn Asn Gly Gln Gln Thr Ala Phe Lys Asp Gly Asp Arg Leu 
                485                 490                 495     


Thr Ile Gly Lys Gly Asp Pro Ile Gly Thr Thr Tyr Asn Ile Lys Leu 
            500                 505                 510         


Thr Gly Thr Asn Gly Glu Gly Ala Ala Arg Thr Gln Glu Tyr Thr Phe 
        515                 520                 525             


Val Lys Lys Asp Pro Ser Gln Thr Asn Ile Ile Gly Tyr Gln Asn Pro 
    530                 535                 540                 


Asp His Trp Gly Gln Val Asn Ala Tyr Ile Tyr Lys His Asp Gly Gly 
545                 550                 555                 560 


Arg Ala Ile Glu Leu Thr Gly Ser Trp Pro Gly Lys Ala Met Thr Lys 
                565                 570                 575     


Asn Ala Asn Gly Met Tyr Thr Leu Thr Leu Pro Glu Asn Thr Asp Thr 
            580                 585                 590         


Ala Asn Ala Lys Val Ile Phe Asn Asn Gly Ser Ala Gln Val Pro Gly 
        595                 600                 605             


Gln Asn Gln Pro Gly Phe Asp Tyr Val Gln Asn Gly Leu Tyr Asn Asn 
    610                 615                 620                 


Ser Gly Leu Asn Gly Tyr Leu Pro His Thr Gly Gly Thr Thr Thr Thr 
625                 630                 635                 640 


Ala Thr Pro Thr Gly Ser Gly Ser Val Thr Ser Thr Ser Lys Thr Thr 
                645                 650                 655     


Ala Thr Ala Ser Lys Thr Ser Thr Ser Thr Ser Ser Thr Ser Cys Thr 
            660                 665                 670         


Thr Pro Thr Ala Val Ala Val Thr Phe Asp Leu Thr Ala Thr Thr Thr 
        675                 680                 685             


Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser Ile Ser Gln Leu Gly Asp 
    690                 695                 700                 


Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser Ala Asp Lys Tyr Thr Ser 
705                 710                 715                 720 


Ser Asp Pro Leu Trp Tyr Val Thr Val Thr Leu Pro Ala Gly Glu Ser 
                725                 730                 735     


Phe Glu Tyr Lys Phe Ile Arg Ile Glu Ser Asp Asp Ser Val Glu Trp 
            740                 745                 750         


Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val Pro Gln Ala Cys Gly Thr 
        755                 760                 765             


Ser Thr Ala Thr Val Thr Asp Thr Trp Arg 
    770                 775             


<210>  9
<211>  515
<212>  PRT
<213>  Saccharomycopsis fibuligera

<400>  9

Met Ile Arg Leu Thr Val Phe Leu Thr Ala Val Phe Ala Ala Val Ala 
1               5                   10                  15      


Ser Cys Val Pro Val Glu Leu Asp Lys Arg Asn Thr Gly His Phe Gln 
            20                  25                  30          


Ala Tyr Ser Gly Tyr Thr Val Ala Arg Ser Asn Phe Thr Gln Trp Ile 
        35                  40                  45              


His Glu Gln Pro Ala Val Ser Trp Tyr Tyr Leu Leu Gln Asn Ile Asp 
    50                  55                  60                  


Tyr Pro Glu Gly Gln Phe Lys Ser Ala Lys Pro Gly Val Val Val Ala 
65                  70                  75                  80  


Ser Pro Ser Thr Ser Glu Pro Asp Tyr Phe Tyr Gln Trp Thr Arg Asp 
                85                  90                  95      


Thr Ala Ile Thr Phe Leu Ser Leu Ile Ala Glu Val Glu Asp His Ser 
            100                 105                 110         


Phe Ser Asn Thr Thr Leu Ala Lys Val Val Glu Tyr Tyr Ile Ser Asn 
        115                 120                 125             


Thr Tyr Thr Leu Gln Arg Val Ser Asn Pro Ser Gly Asn Phe Asp Ser 
    130                 135                 140                 


Pro Asn His Asp Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Asp Thr 
145                 150                 155                 160 


Ala Tyr Thr Ala Ser Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Leu 
                165                 170                 175     


Arg Ala Tyr Ala Ile Ser Arg Tyr Leu Asn Ala Val Ala Lys His Asn 
            180                 185                 190         


Asn Gly Lys Leu Leu Leu Ala Gly Gln Asn Gly Ile Pro Tyr Ser Ser 
        195                 200                 205             


Ala Ser Asp Ile Tyr Trp Lys Ile Ile Lys Pro Asp Leu Gln His Val 
    210                 215                 220                 


Ser Thr His Trp Ser Thr Ser Gly Phe Asp Leu Trp Glu Glu Asn Gln 
225                 230                 235                 240 


Gly Thr His Phe Phe Thr Ala Leu Val Gln Leu Lys Ala Leu Ser Tyr 
                245                 250                 255     


Gly Ile Pro Leu Ser Lys Thr Tyr Asn Asp Pro Gly Phe Thr Ser Trp 
            260                 265                 270         


Leu Glu Lys Gln Lys Asp Ala Leu Asn Ser Tyr Ile Asn Ser Ser Gly 
        275                 280                 285             


Phe Val Asn Ser Gly Lys Lys His Ile Val Glu Ser Pro Gln Leu Ser 
    290                 295                 300                 


Ser Arg Gly Gly Leu Asp Ser Ala Thr Tyr Ile Ala Ala Leu Ile Thr 
305                 310                 315                 320 


His Asp Ile Gly Asp Asp Asp Thr Tyr Thr Pro Phe Asn Val Asp Asn 
                325                 330                 335     


Ser Tyr Val Leu Asn Ser Leu Tyr Tyr Leu Leu Val Asp Asn Lys Asn 
            340                 345                 350         


Arg Tyr Lys Ile Asn Gly Asn Tyr Lys Ala Gly Ala Ala Val Gly Arg 
        355                 360                 365             


Tyr Pro Glu Asp Val Tyr Asn Gly Val Gly Thr Ser Glu Gly Asn Pro 
    370                 375                 380                 


Trp Gln Leu Ala Thr Ala Tyr Ala Gly Gln Thr Phe Tyr Thr Leu Ala 
385                 390                 395                 400 


Tyr Asn Ser Leu Lys Asn Lys Lys Asn Leu Val Ile Glu Lys Leu Asn 
                405                 410                 415     


Tyr Asp Leu Tyr Asn Ser Phe Ile Ala Asp Leu Ser Lys Ile Asp Ser 
            420                 425                 430         


Ser Tyr Ala Ser Lys Asp Ser Leu Thr Leu Thr Tyr Gly Ser Asp Asn 
        435                 440                 445             


Tyr Lys Asn Val Ile Lys Ser Leu Leu Gln Phe Gly Asp Ser Phe Leu 
    450                 455                 460                 


Lys Val Leu Leu Asp His Ile Asp Asp Asn Gly Gln Leu Thr Glu Glu 
465                 470                 475                 480 


Ile Asn Arg Tyr Thr Gly Phe Gln Ala Gly Ala Val Ser Leu Thr Trp 
                485                 490                 495     


Ser Ser Gly Ser Leu Leu Ser Ala Asn Arg Ala Arg Asn Lys Leu Ile 
            500                 505                 510         


Glu Leu Leu 
        515 


<210>  10
<211>  515
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  A40N variant of S. fibuligera glucosamylase

<400>  10

Met Ile Arg Leu Thr Val Phe Leu Thr Ala Val Phe Ala Ala Val Ala 
1               5                   10                  15      


Ser Cys Val Pro Val Glu Leu Asp Lys Arg Asn Thr Gly His Phe Gln 
            20                  25                  30          


Ala Tyr Ser Gly Tyr Thr Val Asn Arg Ser Asn Phe Thr Gln Trp Ile 
        35                  40                  45              


His Glu Gln Pro Ala Val Ser Trp Tyr Tyr Leu Leu Gln Asn Ile Asp 
    50                  55                  60                  


Tyr Pro Glu Gly Gln Phe Lys Ser Ala Lys Pro Gly Val Val Val Ala 
65                  70                  75                  80  


Ser Pro Ser Thr Ser Glu Pro Asp Tyr Phe Tyr Gln Trp Thr Arg Asp 
                85                  90                  95      


Thr Ala Ile Thr Phe Leu Ser Leu Ile Ala Glu Val Glu Asp His Ser 
            100                 105                 110         


Phe Ser Asn Thr Thr Leu Ala Lys Val Val Glu Tyr Tyr Ile Ser Asn 
        115                 120                 125             


Thr Tyr Thr Leu Gln Arg Val Ser Asn Pro Ser Gly Asn Phe Asp Ser 
    130                 135                 140                 


Pro Asn His Asp Gly Leu Gly Glu Pro Lys Phe Asn Val Asp Asp Thr 
145                 150                 155                 160 


Ala Tyr Thr Ala Ser Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Leu 
                165                 170                 175     


Arg Ala Tyr Ala Ile Ser Arg Tyr Leu Asn Ala Val Ala Lys His Asn 
            180                 185                 190         


Asn Gly Lys Leu Leu Leu Ala Gly Gln Asn Gly Ile Pro Tyr Ser Ser 
        195                 200                 205             


Ala Ser Asp Ile Tyr Trp Lys Ile Ile Lys Pro Asp Leu Gln His Val 
    210                 215                 220                 


Ser Thr His Trp Ser Thr Ser Gly Phe Asp Leu Trp Glu Glu Asn Gln 
225                 230                 235                 240 


Gly Thr His Phe Phe Thr Ala Leu Val Gln Leu Lys Ala Leu Ser Tyr 
                245                 250                 255     


Gly Ile Pro Leu Ser Lys Thr Tyr Asn Asp Pro Gly Phe Thr Ser Trp 
            260                 265                 270         


Leu Glu Lys Gln Lys Asp Ala Leu Asn Ser Tyr Ile Asn Ser Ser Gly 
        275                 280                 285             


Phe Val Asn Ser Gly Lys Lys His Ile Val Glu Ser Pro Gln Leu Ser 
    290                 295                 300                 


Ser Arg Gly Gly Leu Asp Ser Ala Thr Tyr Ile Ala Ala Leu Ile Thr 
305                 310                 315                 320 


His Asp Ile Gly Asp Asp Asp Thr Tyr Thr Pro Phe Asn Val Asp Asn 
                325                 330                 335     


Ser Tyr Val Leu Asn Ser Leu Tyr Tyr Leu Leu Val Asp Asn Lys Asn 
            340                 345                 350         


Arg Tyr Lys Ile Asn Gly Asn Tyr Lys Ala Gly Ala Ala Val Gly Arg 
        355                 360                 365             


Tyr Pro Glu Asp Val Tyr Asn Gly Val Gly Thr Ser Glu Gly Asn Pro 
    370                 375                 380                 


Trp Gln Leu Ala Thr Ala Tyr Ala Gly Gln Thr Phe Tyr Thr Leu Ala 
385                 390                 395                 400 


Tyr Asn Ser Leu Lys Asn Lys Lys Asn Leu Val Ile Glu Lys Leu Asn 
                405                 410                 415     


Tyr Asp Leu Tyr Asn Ser Phe Ile Ala Asp Leu Ser Lys Ile Asp Ser 
            420                 425                 430         


Ser Tyr Ala Ser Lys Asp Ser Leu Thr Leu Thr Tyr Gly Ser Asp Asn 
        435                 440                 445             


Tyr Lys Asn Val Ile Lys Ser Leu Leu Gln Phe Gly Asp Ser Phe Leu 
    450                 455                 460                 


Lys Val Leu Leu Asp His Ile Asp Asp Asn Gly Gln Leu Thr Glu Glu 
465                 470                 475                 480 


Ile Asn Arg Tyr Thr Gly Phe Gln Ala Gly Ala Val Ser Leu Thr Trp 
                485                 490                 495     


Ser Ser Gly Ser Leu Leu Ser Ala Asn Arg Ala Arg Asn Lys Leu Ile 
            500                 505                 510         


Glu Leu Leu 
        515 


<210>  11
<211>  519
<212>  PRT
<213>  Saccharomycopsis fibuligera

<400>  11

Met Arg Phe Gly Val Leu Ile Ser Val Phe Ala Ala Ile Val Ser Ala 
1               5                   10                  15      


Leu Pro Leu Gln Glu Gly Pro Leu Asn Lys Arg Ala Tyr Pro Ser Phe 
            20                  25                  30          


Glu Ala Tyr Ser Asn Tyr Lys Val Asp Arg Thr Asp Leu Glu Thr Phe 
        35                  40                  45              


Leu Asp Lys Gln Lys Glu Val Ser Leu Tyr Tyr Leu Leu Gln Asn Ile 
    50                  55                  60                  


Ala Tyr Pro Glu Gly Gln Phe Asn Asn Gly Val Pro Gly Thr Val Ile 
65                  70                  75                  80  


Ala Ser Pro Ser Thr Ser Asn Pro Asp Tyr Tyr Tyr Gln Trp Thr Arg 
                85                  90                  95      


Asp Ser Ala Ile Thr Phe Leu Thr Val Leu Ser Glu Leu Glu Asp Asn 
            100                 105                 110         


Asn Phe Asn Thr Thr Leu Ala Lys Ala Val Glu Tyr Tyr Ile Asn Thr 
        115                 120                 125             


Ser Tyr Asn Leu Gln Arg Thr Ser Asn Pro Ser Gly Ser Phe Asp Asp 
    130                 135                 140                 


Glu Asn His Lys Gly Leu Gly Glu Pro Lys Phe Asn Thr Asp Gly Ser 
145                 150                 155                 160 


Ala Tyr Thr Gly Ala Trp Gly Arg Pro Gln Asn Asp Gly Pro Ala Leu 
                165                 170                 175     


Arg Ala Tyr Ala Ile Ser Arg Tyr Leu Asn Asp Val Asn Ser Leu Asn 
            180                 185                 190         


Glu Gly Lys Leu Val Leu Thr Asp Ser Gly Gly Ile Asn Phe Ser Ser 
        195                 200                 205             


Thr Glu Asp Ile Tyr Lys Asn Ile Ile Lys Pro Asp Leu Glu Tyr Val 
    210                 215                 220                 


Ile Gly Tyr Trp Asp Ser Thr Gly Phe Asp Leu Trp Glu Glu Asn Gln 
225                 230                 235                 240 


Gly Arg His Phe Phe Thr Ser Leu Val Gln Gln Lys Ala Leu Ala Tyr 
                245                 250                 255     


Ala Val Asp Ile Ala Lys Ser Phe Asp Asp Gly Asp Phe Ala Asn Thr 
            260                 265                 270         


Leu Ser Ser Thr Ala Ser Thr Leu Glu Ser Tyr Leu Ser Gly Ser Asp 
        275                 280                 285             


Gly Gly Phe Val Asn Thr Asp Val Asn His Ile Val Glu Asn Pro Asp 
    290                 295                 300                 


Leu Leu Gln Gln Asn Ser Arg Gln Gly Leu Asp Ser Ala Thr Tyr Ile 
305                 310                 315                 320 


Gly Pro Leu Leu Thr His Asp Ile Gly Glu Ser Ser Ser Thr Pro Phe 
                325                 330                 335     


Asp Val Asp Asn Glu Tyr Val Leu Gln Ser Tyr Tyr Leu Leu Leu Glu 
            340                 345                 350         


Asp Asn Lys Asp Arg Tyr Phe Val Asn Ser Ala Tyr Ser Ala Gly Ala 
        355                 360                 365             


Ala Ile Gly Arg Tyr Pro Glu Asp Val Tyr Asn Gly Asp Gly Ser Ser 
    370                 375                 380                 


Glu Gly Asn Pro Trp Phe Leu Ala Thr Ala Tyr Ala Ala Gln Val Pro 
385                 390                 395                 400 


Tyr Lys Leu Ala Tyr Asp Ala Lys Ser Ala Ser Asn Asp Ile Thr Ile 
                405                 410                 415     


Asn Lys Ile Asn Tyr Asp Phe Phe Asn Lys Tyr Ile Val Asp Leu Ser 
            420                 425                 430         


Thr Ile Asn Ser Ala Tyr Gln Ser Ser Asp Ser Val Thr Ile Lys Ser 
        435                 440                 445             


Gly Ser Asp Glu Phe Asn Thr Val Ala Asp Asn Leu Val Thr Phe Gly 
    450                 455                 460                 


Asp Ser Phe Leu Gln Val Ile Leu Asp His Ile Asn Asp Asp Gly Ser 
465                 470                 475                 480 


Leu Asn Glu Gln Leu Asn Arg Tyr Thr Gly Tyr Ser Thr Gly Ala Tyr 
                485                 490                 495     


Ser Leu Thr Trp Ser Ser Gly Ala Leu Leu Glu Ala Ile Arg Leu Arg 
            500                 505                 510         


Asn Lys Val Lys Ala Leu Ala 
        515                 


<210>  12
<211>  626
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  B. amyloliquefaciens amyE alpha-amylase with modified N-terminus

<400>  12

Glu Thr Ala Asn Lys Ser Asn Lys Val Thr Ala Ser Ser Val Lys Asn 
1               5                   10                  15      


Gly Thr Ile Leu His Ala Trp Asn Trp Ser Phe Asn Thr Leu Thr Gln 
            20                  25                  30          


Asn Met Lys Asp Ile Arg Asp Ala Gly Tyr Ala Ala Ile Gln Thr Ser 
        35                  40                  45              


Pro Ile Asn Gln Val Lys Glu Gly Asn Gln Gly Asp Lys Ser Met Arg 
    50                  55                  60                  


Asn Trp Tyr Trp Leu Tyr Gln Pro Thr Ser Tyr Gln Ile Gly Asn Arg 
65                  70                  75                  80  


Tyr Leu Gly Thr Glu Gln Glu Phe Lys Asp Met Cys Ala Ala Ala Glu 
                85                  90                  95      


Lys Tyr Gly Val Lys Val Ile Val Asp Ala Val Ile Asn His Thr Thr 
            100                 105                 110         


Ser Asp Tyr Gly Ala Ile Ser Asp Glu Ile Lys Arg Ile Pro Asn Trp 
        115                 120                 125             


Thr His Gly Asn Thr Gln Ile Lys Asn Trp Ser Asp Arg Trp Asp Val 
    130                 135                 140                 


Thr Gln Asn Ser Leu Leu Gly Leu Tyr Asp Trp Asn Thr Gln Asn Thr 
145                 150                 155                 160 


Glu Val Gln Val Tyr Leu Lys Arg Phe Leu Glu Arg Ala Leu Asn Asp 
                165                 170                 175     


Gly Ala Asp Gly Phe Arg Tyr Asp Ala Ala Lys His Ile Glu Leu Pro 
            180                 185                 190         


Asp Asp Gly Asn Tyr Gly Ser Gln Phe Trp Pro Asn Ile Thr Asn Thr 
        195                 200                 205             


Ser Ala Glu Phe Gln Tyr Gly Glu Ile Leu Gln Asp Ser Ala Ser Arg 
    210                 215                 220                 


Asp Thr Ala Tyr Ala Asn Tyr Met Asn Val Thr Ala Ser Asn Tyr Gly 
225                 230                 235                 240 


His Ser Ile Arg Ser Ala Leu Lys Asn Arg Asn Leu Ser Val Ser Asn 
                245                 250                 255     


Ile Ser His Tyr Ala Ser Asp Val Ser Ala Asp Lys Leu Val Thr Trp 
            260                 265                 270         


Val Glu Ser His Asp Thr Tyr Ala Asn Asp Asp Glu Glu Ser Thr Trp 
        275                 280                 285             


Met Ser Asp Asp Asp Ile Arg Leu Gly Trp Ala Val Ile Gly Ser Arg 
    290                 295                 300                 


Ser Gly Ser Thr Pro Leu Phe Phe Ser Arg Pro Glu Gly Gly Gly Asn 
305                 310                 315                 320 


Gly Val Arg Phe Pro Gly Lys Ser Gln Ile Gly Asp Arg Gly Ser Ala 
                325                 330                 335     


Leu Phe Lys Asp Gln Ala Ile Thr Ala Val Asn Thr Phe His Asn Val 
            340                 345                 350         


Met Ala Gly Gln Pro Glu Glu Leu Ser Asn Pro Asn Gly Asn Asn Gln 
        355                 360                 365             


Val Phe Met Asn Gln Arg Gly Ser Lys Gly Val Val Leu Ala Asn Ala 
    370                 375                 380                 


Gly Ser Ser Ser Val Thr Ile Asn Thr Ser Ala Lys Leu Pro Asp Gly 
385                 390                 395                 400 


Arg Tyr Asp Asn Arg Ala Gly Ala Gly Ser Phe Gln Val Ala Asn Gly 
                405                 410                 415     


Lys Leu Thr Gly Thr Ile Asn Ala Arg Ser Ala Ala Val Leu Tyr Pro 
            420                 425                 430         


Asp Asp Ile Gly Asn Ala Pro His Val Phe Leu Glu Asn Tyr Gln Thr 
        435                 440                 445             


Gly Ala Val His Ser Phe Asn Asp Gln Leu Thr Val Thr Leu Arg Ala 
    450                 455                 460                 


Asn Ala Lys Thr Thr Lys Ala Val Tyr Gln Ile Asn Asn Gly Gln Gln 
465                 470                 475                 480 


Thr Ala Phe Lys Asp Gly Asp Arg Leu Thr Ile Gly Lys Gly Asp Pro 
                485                 490                 495     


Ile Gly Thr Thr Tyr Asn Ile Lys Leu Thr Gly Thr Asn Gly Glu Gly 
            500                 505                 510         


Ala Ala Arg Thr Gln Glu Tyr Thr Phe Val Lys Lys Asp Pro Ser Gln 
        515                 520                 525             


Thr Asn Ile Ile Gly Tyr Gln Asn Pro Asp His Trp Gly Gln Val Asn 
    530                 535                 540                 


Ala Tyr Ile Tyr Lys His Asp Gly Gly Arg Ala Ile Glu Leu Thr Gly 
545                 550                 555                 560 


Ser Trp Pro Gly Lys Ala Met Thr Lys Asn Ala Asn Gly Met Tyr Thr 
                565                 570                 575     


Leu Thr Leu Pro Glu Asn Thr Asp Thr Ala Asn Ala Lys Val Ile Phe 
            580                 585                 590         


Asn Asn Gly Ser Ala Gln Val Pro Gly Gln Asn Gln Pro Gly Phe Asp 
        595                 600                 605             


Tyr Val Gln Asn Gly Leu Tyr Asn Asn Ser Gly Leu Asn Gly Tyr Leu 
    610                 615                 620                 


Pro His 
625     


<210>  13
<211>  19
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  13

Met Leu Leu Gln Ala Phe Leu Phe Leu Leu Ala Gly Phe Ala Ala Lys 
1               5                   10                  15      


Ile Ser Ala 
            


<210>  14
<211>  18
<212>  PRT
<213>  Saccharomyces cerevisiae

<400>  14

Met Gln Leu Leu Arg Cys Phe Ser Ile Phe Ser Val Ile Ala Ser Val 
1               5                   10                  15      


Leu Ala 
        


<210>  15
<211>  20
<212>  PRT
<213>  Aspergillus terreus

<400>  15

Met Lys Trp Thr Phe Ser Leu Leu Leu Leu Leu Ser Val Phe Gly Gln 
1               5                   10                  15      


Ala Thr His Ala 
            20  


<210>  16
<211>  37
<212>  PRT
<213>  Aspergillus niger

<400>  16

Thr Gly Gly Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val 
1               5                   10                  15      


Thr Ser Thr Ser Lys Thr Thr Ala Thr Ala Ser Lys Thr Ser Thr Ser 
            20                  25                  30          


Thr Ser Ser Thr Ser 
        35          


<210>  17
<211>  38
<212>  PRT
<213>  Aspergillus niger

<400>  17

Ala Thr Gly Gly Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser 
1               5                   10                  15      


Val Thr Ser Thr Ser Lys Thr Thr Ala Thr Ala Ser Lys Thr Ser Thr 
            20                  25                  30          


Ser Thr Ser Ser Thr Ser 
        35              


<210>  18
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  18

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser 
1               5                   10                  15  


<210>  19
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  19

Gly Gly Gly Gly Gly Gly Gly Gly 
1               5               


<210>  20
<211>  40
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  20

Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly 
1               5                   10                  15      


Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly Gly Gly Ser Gly Gly 
            20                  25                  30          


Gly Gly Ser Gly Gly Gly Gly Ser 
        35                  40  


<210>  21
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  21

Gly Ser Ala Gly Ser Ala Ala Gly Ser Gly Glu Phe 
1               5                   10          


<210>  22
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  22

Glu Ala Ala Lys Glu Ala Ala Lys Glu Ala Ala Lys 
1               5                   10          


<210>  23
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  23

Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro Ala Pro 
1               5                   10                  15      


Ala Pro Ala Pro 
            20  


<210>  24
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Linker

<400>  24

Ala Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys 
1               5                   10                  15      


Glu Ala Ala Ala Lys Ala Leu Glu Ala Glu Ala Ala Ala Lys Glu Ala 
            20                  25                  30          


Ala Ala Lys Glu Ala Ala Ala Lys Glu Ala Ala Ala Lys Ala 
        35                  40                  45      


