                         SEQUENCE LISTING

<110>  Stellenbosch University
 
<120>  Enzyme-Polymer Conjugate

<130>  P3478PC00-QT4NP

<150>  GB1805086.4
<151>  2018-03-28

<160>  5     

<170>  PatentIn version 3.5

<210>  1
<211>  639
<212>  PRT
<213>  Aspergillus niger


<220>
<221>  Glucoamylase
<222>  (1)..(639)

<400>  1

Met Ser Phe Arg Ser Leu Leu Ala Leu Ser Gly Leu Val Cys Thr Gly 
1               5                   10                  15      


Leu Ala Asn Val Ile Ser Lys Arg Ala Thr Trp Asp Ser Trp Leu Ser 
            20                  25                  30          


Asn Glu Ala Thr Val Ala Arg Thr Ala Ile Leu Asn Asn Ile Gly Ala 
        35                  40                  45              


Asp Gly Ala Trp Val Ser Gly Ala Asp Ser Gly Ile Val Val Ala Ser 
    50                  55                  60                  


Pro Ser Thr Asp Asn Pro Asp Tyr Phe Tyr Thr Trp Thr Arg Asp Ser 
65                  70                  75                  80  


Gly Leu Val Leu Lys Thr Leu Val Asp Leu Phe Arg Asn Gly Asp Thr 
                85                  90                  95      


Ser Leu Leu Ser Thr Ile Glu Asn Tyr Ile Ser Ala Gln Ala Ile Val 
            100                 105                 110         


Gln Gly Ile Ser Asn Pro Ser Gly Asp Leu Ser Ser Gly Ala Gly Leu 
        115                 120                 125             


Gly Glu Pro Lys Phe Asn Val Asp Glu Thr Ala Tyr Thr Gly Ser Trp 
    130                 135                 140                 


Gly Arg Pro Gln Arg Asp Gly Pro Ala Leu Arg Ala Thr Ala Met Ile 
145                 150                 155                 160 


Gly Phe Gly Gln Trp Leu Leu Asp Asn Gly Tyr Thr Ser Thr Ala Thr 
                165                 170                 175     


Asp Ile Val Trp Pro Leu Val Arg Asn Asp Leu Ser Tyr Val Ala Gln 
            180                 185                 190         


Tyr Trp Asn Gln Thr Gly Tyr Asp Leu Trp Glu Val Asn Gly Ser Ser 
        195                 200                 205             


Phe Phe Thr Ile Ala Val Gln His Arg Ala Leu Val Glu Gly Ser Ala 
    210                 215                 220                 


Phe Ala Thr Ala Val Gly Ser Ser Cys Ser Trp Cys Asp Ser Gln Ala 
225                 230                 235                 240 


Pro Glu Ile Leu Cys Tyr Leu Gln Ser Phe Trp Thr Gly Ser Phe Ile 
                245                 250                 255     


Leu Ala Asn Phe Asp Ser Ser Arg Ser Ala Lys Asp Ala Asn Thr Leu 
            260                 265                 270         


Leu Leu Gly Ser Ile His Thr Phe Asp Pro Glu Ala Ala Cys Asp Asp 
        275                 280                 285             


Ser Thr Phe Gln Pro Cys Ser Pro Arg Ala Leu Ala Asn His Lys Glu 
    290                 295                 300                 


Val Val Asp Ser Phe Arg Ser Ile Tyr Thr Leu Asn Asp Gly Leu Ser 
305                 310                 315                 320 


Asp Ser Glu Ala Val Ala Val Gly Arg Tyr Pro Glu Asp Thr Tyr Tyr 
                325                 330                 335     


Asn Gly Asn Pro Trp Phe Leu Cys Thr Leu Ala Ala Ala Glu Gln Leu 
            340                 345                 350         


Tyr Asp Ala Leu Tyr Gln Trp Asp Lys Gln Gly Ser Leu Glu Val Thr 
        355                 360                 365             


Asp Val Ser Leu Asp Phe Phe Lys Ala Leu Tyr Ser Asp Ala Thr Gly 
    370                 375                 380                 


Thr Tyr Ser Ser Ser Ser Ser Thr Tyr Ser Ser Ile Val Asp Ala Val 
385                 390                 395                 400 


Lys Thr Phe Ala Asp Gly Phe Val Ser Ile Val Glu Thr His Ala Ala 
                405                 410                 415     


Ser Asn Gly Ser Met Ser Glu Gln Tyr Asp Lys Ser Asp Gly Glu Gln 
            420                 425                 430         


Leu Ser Ala Arg Asp Leu Thr Trp Ser Tyr Ala Ala Leu Leu Thr Ala 
        435                 440                 445             


Asn Asn Arg Arg Asn Val Val Pro Ser Ala Ser Trp Gly Glu Thr Ser 
    450                 455                 460                 


Ala Ser Ser Val Pro Gly Thr Cys Ala Ala Thr Ser Ala Ile Gly Thr 
465                 470                 475                 480 


Tyr Ser Ser Val Thr Val Thr Ser Trp Pro Ser Ile Val Ala Thr Gly 
                485                 490                 495     


Gly Thr Thr Thr Thr Ala Thr Pro Thr Gly Ser Gly Ser Val Thr Ser 
            500                 505                 510         


Thr Ser Lys Thr Thr Ala Thr Ala Ser Lys Thr Ser Thr Ser Thr Ser 
        515                 520                 525             


Ser Thr Ser Cys Thr Thr Pro Thr Ala Val Ala Val Thr Phe Asp Leu 
    530                 535                 540                 


Thr Ala Thr Thr Thr Tyr Gly Glu Asn Ile Tyr Leu Val Gly Ser Ile 
545                 550                 555                 560 


Ser Gln Leu Gly Asp Trp Glu Thr Ser Asp Gly Ile Ala Leu Ser Ala 
                565                 570                 575     


Asp Lys Tyr Thr Ser Ser Asp Pro Leu Trp Tyr Val Thr Val Thr Leu 
            580                 585                 590         


Pro Ala Gly Glu Ser Phe Glu Tyr Lys Phe Ile Arg Ile Glu Ser Asp 
        595                 600                 605             


Asp Ser Val Glu Trp Glu Ser Asp Pro Asn Arg Glu Tyr Thr Val Pro 
    610                 615                 620                 


Gln Ala Cys Gly Thr Ser Thr Ala Thr Val Thr Asp Thr Trp Arg 
625                 630                 635                 


<210>  2
<211>  853
<212>  PRT
<213>  Bacillus cereus


<220>
<221>  Pullulanase
<222>  (1)..(853)

<400>  2

Met Val Gln Ile Thr Lys Arg Leu Ile Asn Lys Thr Val Leu Leu Leu 
1               5                   10                  15      


Thr Leu Ile Val Met Leu Ser Ser Val Phe Ser Phe Gln Asn Val Lys 
            20                  25                  30          


Ala Val Ser Asn Ser Lys Thr Thr Glu Val Ile Ile His Tyr Lys Glu 
        35                  40                  45              


Gln Ser Gly Asn Thr Lys Asp Trp Asn Leu Trp Ile Trp Gly Glu Asn 
    50                  55                  60                  


Ser Ser Gly Lys Ser Tyr Glu Phe Thr Gly Glu Asp Glu Phe Gly Lys 
65                  70                  75                  80  


Tyr Ala Lys Ile Asn Ile Asp Gly Asp Tyr Asn Arg Leu Gly Phe Ile 
                85                  90                  95      


Ile Arg Thr Asn Glu Trp Glu Lys Asp Gly Gly Asp Arg Trp Ile Glu 
            100                 105                 110         


Asn Ile Lys Asp Gly Arg Ala Glu Val Trp Ile Leu Ser Gly Asp Glu 
        115                 120                 125             


Lys Val Tyr Asn Ser Lys Pro Ser Ser Asp Leu Ser Ile Gln Lys Ala 
    130                 135                 140                 


Thr Ile Asp Ser Phe His Glu Ile Thr Val Thr Thr Asn Val Pro Phe 
145                 150                 155                 160 


His Ile Lys Glu Lys Lys Ile Glu Met Glu Gly Ile Lys Ile Lys Asn 
                165                 170                 175     


Ile Thr Pro Tyr Asp Ile Asn Ser Gly Asp Ile Thr Asn Lys Val Lys 
            180                 185                 190         


Ile Ile Thr Glu Gln Lys Ile Asp Leu Lys Gln Thr Tyr Lys Val Lys 
        195                 200                 205             


Ile Glu Asn Leu Ala Asp Thr His Thr Glu Ile Gly Lys Val Ile Arg 
    210                 215                 220                 


Thr Glu Glu Phe Asp Lys Leu Phe Tyr Tyr Gly Gly Asn Asp Leu Gly 
225                 230                 235                 240 


Asn Ile Tyr Thr Pro Gln His Thr Lys Phe Arg Val Trp Ala Pro Thr 
                245                 250                 255     


Ala Ser Glu Ala Lys Leu Val Thr Tyr Lys Lys Trp Asn Asp Lys Ile 
            260                 265                 270         


Gly Thr Glu Ile Asn Met Gln Gln Gly Glu Lys Gly Thr Trp Lys Ala 
        275                 280                 285             


Glu Leu Lys Gly Asn Gln Lys Gly Leu Tyr Tyr Thr Tyr Lys Val Lys 
    290                 295                 300                 


Ile Gly Asp Lys Trp Thr Glu Ala Val Asp Pro Tyr Val Arg Ala Ala 
305                 310                 315                 320 


Ser Val Asn Gly Asp Lys Gly Ala Val Val Asp Leu Glu Glu Thr Asn 
                325                 330                 335     


Pro Lys Arg Trp Asn Thr Asn Lys Lys Pro Lys Leu Lys Asn Pro Glu 
            340                 345                 350         


Asp Ala Ile Ile Tyr Glu Leu His Val Arg Asp Leu Ser Ile Gln Pro 
        355                 360                 365             


Glu Ser Gly Ile Lys Gln Lys Gly Lys Tyr Leu Gly Val Thr Glu Lys 
    370                 375                 380                 


Gly Thr Lys Gly Pro Glu Gly Val Lys Thr Gly Leu Asp His Met Lys 
385                 390                 395                 400 


Asp Leu Gly Val Thr His Val Gln Leu Leu Pro Ile Phe Asp Tyr Ala 
                405                 410                 415     


Ser Val Asn Glu Glu Lys Val Asn Glu Pro Gln Tyr Asn Trp Gly Tyr 
            420                 425                 430         


Asp Pro Lys Asn Phe Asn Val Pro Glu Gly Ser Tyr Ser Thr Asn Pro 
        435                 440                 445             


Tyr Glu Pro Thr Val Arg Ile Thr Glu Leu Lys Gln Met Ile Gln Thr 
    450                 455                 460                 


Leu His Asp Asn Asn Leu Arg Val Val Met Asp Val Val Tyr Asn His 
465                 470                 475                 480 


Met Tyr Asn Ala Val Glu Ser Asn Phe His Lys Leu Val Pro Gly Tyr 
                485                 490                 495     


Tyr Tyr Arg Tyr Asn Glu Asp Gly Thr Phe Ala Asn Gly Thr Gly Val 
            500                 505                 510         


Gly Asn Asp Thr Ala Ser Glu Arg Lys Met Met Arg Lys Phe Met Ile 
        515                 520                 525             


Asp Ser Val Thr Tyr Trp Ala Lys Glu Tyr Asn Leu Asp Gly Phe Arg 
    530                 535                 540                 


Phe Asp Leu Met Gly Ile His Asp Tyr Glu Thr Val Asn Glu Ile Arg 
545                 550                 555                 560 


Lys Ala Val Asn Gln Ile Asp Pro Ser Ile Ile Leu His Gly Glu Gly 
                565                 570                 575     


Trp Asn Leu Asn Thr Pro Leu Ala Ala Glu Leu Lys Ala Asn Gln Lys 
            580                 585                 590         


Asn Ala Glu Lys Met Lys Gly Ile Ala His Phe Asn Asp Asn Ile Arg 
        595                 600                 605             


Asp Gly Leu Lys Gly Ser Val Phe Glu Glu Lys Glu Asn Gly Phe Val 
    610                 615                 620                 


Asn Gly Lys Glu Asn Met Glu Asp Arg Ile Lys Lys Gly Ile Thr Ala 
625                 630                 635                 640 


Gly Ile Asp Tyr Asp Arg Asn Thr Ser Thr Tyr Gln Asp Pro Glu Gln 
                645                 650                 655     


Val Leu Thr Tyr Val Glu Ala His Asp Asn His Thr Leu Trp Asp Lys 
            660                 665                 670         


Leu Glu Leu Thr Asn Pro Gly Asp Ser Glu Glu Ala Arg Lys Gln Met 
        675                 680                 685             


His Lys Leu Ser Ser Ser Ile Leu Leu Thr Ser Gln Gly Ile Pro Phe 
    690                 695                 700                 


Leu His Ala Gly Gln Glu Phe Met Arg Thr Lys Tyr Gly Asp His Asn 
705                 710                 715                 720 


Ser Tyr Lys Ser Pro Asp Ser Ile Asn Gln Met Asp Trp Leu Arg Arg 
                725                 730                 735     


Ala Ala Phe Asn Asn Glu Val Asp Tyr Met Lys Gly Leu Ile Glu Leu 
            740                 745                 750         


Arg Lys Lys Tyr Pro Ala Phe Arg Met Thr Ser Ala Glu Gln Ile Lys 
        755                 760                 765             


Thr His Val Ser Phe Ile Asp Ala Pro Lys Asn Thr Val Ala Tyr Thr 
    770                 775                 780                 


Ile Glu Gly Asn Lys Asn Glu Tyr Phe Thr Val Ala His Asn Ala Asn 
785                 790                 795                 800 


Lys Glu Ala Gly Glu Ile Thr Leu Pro Ser Lys Gly Pro Trp Lys Val 
                805                 810                 815     


Leu Val Asp Gly Lys Gln Ala Gly Ser Lys Pro Leu Tyr Val Val His 
            820                 825                 830         


Asp Asn Lys Ile Lys Val Pro Ala Leu Ser Ser Leu Val Leu Lys Thr 
        835                 840                 845             


Glu Lys Pro Ile Lys 
    850             


<210>  3
<211>  381
<212>  PRT
<213>  Bacillus pumilus


<220>
<221>  Subtilisin-like serine protease
<222>  (1)..(381)

<400>  3

Met Lys Lys Lys Asn Val Met Thr Ser Val Leu Leu Ala Val Pro Leu 
1               5                   10                  15      


Leu Phe Ser Ala Gly Phe Gly Gly Ser Met Ala Asn Ala Glu Thr Val 
            20                  25                  30          


Ser Lys Ser Ala Ser Glu Lys Ser Tyr Ile Val Gly Phe Lys Ala Ser 
        35                  40                  45              


Ala Thr Thr Asn Ser Ser Lys Lys Gln Ala Val Thr Gln Asn Gly Gly 
    50                  55                  60                  


Lys Leu Glu Lys Gln Tyr Arg Leu Ile Asn Ala Ala Gln Val Lys Met 
65                  70                  75                  80  


Ser Glu Gln Ala Ala Lys Lys Leu Glu His Asp Pro Ser Ile Ala Tyr 
                85                  90                  95      


Val Glu Glu Asp His Lys Ala Glu Ala Tyr Ala Gln Thr Val Pro Tyr 
            100                 105                 110         


Gly Ile Pro Gln Ile Lys Ala Pro Ala Val His Ala Gln Gly Tyr Lys 
        115                 120                 125             


Gly Ala Asn Val Lys Val Ala Val Leu Asp Thr Gly Ile His Ala Ala 
    130                 135                 140                 


His Pro Asp Leu Asn Val Ala Gly Gly Ala Ser Phe Val Pro Ser Glu 
145                 150                 155                 160 


Pro Asn Ala Thr Gln Asp Phe Gln Ser His Gly Thr His Val Ala Gly 
                165                 170                 175     


Thr Ile Ala Ala Leu Asp Asn Thr Ile Gly Val Leu Gly Val Ala Pro 
            180                 185                 190         


Ser Ala Ser Leu Tyr Ala Val Lys Val Leu Asp Arg Asn Gly Asp Gly 
        195                 200                 205             


Gln Tyr Ser Trp Ile Ile Ser Gly Ile Glu Trp Ala Val Ala Asn Asn 
    210                 215                 220                 


Met Asp Val Ile Asn Met Ser Leu Gly Gly Pro Asn Gly Ser Thr Ala 
225                 230                 235                 240 


Leu Lys Asn Ala Val Asp Thr Ala Asn Asn Arg Gly Val Val Val Val 
                245                 250                 255     


Ala Ala Ala Gly Asn Ser Gly Ser Thr Gly Ser Thr Ser Thr Val Gly 
            260                 265                 270         


Tyr Pro Ala Lys Tyr Asp Ser Thr Ile Ala Val Ala Asn Val Asn Ser 
        275                 280                 285             


Ser Asn Val Arg Asn Ser Ser Ser Ser Ala Gly Pro Glu Leu Asp Val 
    290                 295                 300                 


Ser Ala Pro Gly Thr Ser Ile Leu Ser Thr Val Pro Ser Ser Gly Tyr 
305                 310                 315                 320 


Thr Ser Tyr Thr Gly Thr Ser Met Ala Ser Pro His Val Ala Gly Ala 
                325                 330                 335     


Ala Ala Leu Ile Leu Ser Lys Asn Pro Asn Leu Ser Asn Ser Gln Val 
            340                 345                 350         


Arg Gln Arg Leu Glu Asn Thr Ala Thr Pro Leu Gly Asn Ser Phe Tyr 
        355                 360                 365             


Tyr Gly Lys Gly Leu Ile Asn Ala Gln Ala Ala Ser Asn 
    370                 375                 380     


<210>  4
<211>  1004
<212>  PRT
<213>  Aspergillus oryzae


<220>
<221>  Galactosidase
<222>  (1)..(1004)

<400>  4

Met Lys Leu Leu Ser Val Ala Ala Val Ala Leu Leu Ala Ala Gln Ala 
1               5                   10                  15      


Ala Gly Ala Ser Ile Lys His Arg Leu Asn Gly Phe Thr Ile Leu Glu 
            20                  25                  30          


His Pro Asp Pro Ala Lys Arg Asp Leu Leu Gln Asp Ile Val Thr Trp 
        35                  40                  45              


Asp Asp Lys Ser Leu Phe Ile Asn Gly Glu Arg Ile Met Leu Phe Ser 
    50                  55                  60                  


Gly Glu Val His Pro Phe Arg Leu Pro Val Pro Ser Leu Trp Leu Asp 
65                  70                  75                  80  


Ile Phe His Lys Ile Arg Ala Leu Gly Phe Asn Cys Val Ser Phe Tyr 
                85                  90                  95      


Ile Asp Trp Ala Leu Leu Glu Gly Lys Pro Gly Asp Tyr Arg Ala Glu 
            100                 105                 110         


Gly Ile Phe Ala Leu Glu Pro Phe Phe Asp Ala Ala Lys Glu Ala Gly 
        115                 120                 125             


Ile Tyr Leu Ile Ala Arg Pro Gly Ser Tyr Ile Asn Ala Glu Val Ser 
    130                 135                 140                 


Gly Gly Gly Phe Pro Gly Trp Leu Gln Arg Val Asn Gly Thr Leu Arg 
145                 150                 155                 160 


Ser Ser Asp Glu Pro Phe Leu Lys Ala Thr Asp Asn Tyr Ile Ala Asn 
                165                 170                 175     


Ala Ala Ala Ala Val Ala Lys Ala Gln Ile Thr Asn Gly Gly Pro Val 
            180                 185                 190         


Ile Leu Tyr Gln Pro Glu Asn Glu Tyr Ser Gly Gly Cys Cys Gly Val 
        195                 200                 205             


Lys Tyr Pro Asp Ala Asp Tyr Met Gln Tyr Val Met Asp Gln Ala Arg 
    210                 215                 220                 


Lys Ala Asp Ile Val Val Pro Phe Ile Ser Asn Asp Ala Ser Pro Ser 
225                 230                 235                 240 


Gly His Asn Ala Pro Gly Ser Gly Thr Ser Ala Val Asp Ile Tyr Gly 
                245                 250                 255     


His Asp Ser Tyr Pro Leu Gly Phe Asp Cys Ala Asn Pro Ser Val Trp 
            260                 265                 270         


Pro Glu Gly Lys Leu Pro Asp Asn Phe Arg Thr Leu His Leu Glu Gln 
        275                 280                 285             


Ser Pro Ser Thr Pro Tyr Ser Leu Leu Glu Phe Gln Ala Gly Ala Phe 
    290                 295                 300                 


Asp Pro Trp Gly Gly Pro Gly Phe Glu Lys Cys Tyr Ala Leu Val Asn 
305                 310                 315                 320 


His Glu Phe Ser Arg Val Phe Tyr Arg Asn Asp Leu Ser Phe Gly Val 
                325                 330                 335     


Ser Thr Phe Asn Leu Tyr Met Thr Phe Gly Gly Thr Asn Trp Gly Asn 
            340                 345                 350         


Leu Gly His Pro Gly Gly Tyr Thr Ser Tyr Asp Tyr Gly Ser Pro Ile 
        355                 360                 365             


Thr Glu Thr Arg Asn Val Thr Arg Glu Lys Tyr Ser Asp Ile Lys Leu 
    370                 375                 380                 


Leu Ala Asn Phe Val Lys Ala Ser Pro Ser Tyr Leu Thr Ala Thr Pro 
385                 390                 395                 400 


Arg Asn Leu Thr Thr Gly Val Tyr Thr Asp Thr Ser Asp Leu Ala Val 
                405                 410                 415     


Thr Pro Leu Ile Gly Asp Ser Pro Gly Ser Phe Phe Val Val Arg His 
            420                 425                 430         


Thr Asp Tyr Ser Ser Gln Glu Ser Thr Ser Tyr Lys Leu Lys Leu Pro 
        435                 440                 445             


Thr Ser Ala Gly Asn Leu Thr Ile Pro Gln Leu Glu Gly Thr Leu Ser 
    450                 455                 460                 


Leu Asn Gly Arg Asp Ser Lys Ile His Val Val Asp Tyr Asn Val Ser 
465                 470                 475                 480 


Gly Thr Asn Ile Ile Tyr Ser Thr Ala Glu Val Phe Thr Trp Lys Lys 
                485                 490                 495     


Phe Asp Gly Asn Lys Val Leu Val Leu Tyr Gly Gly Pro Lys Glu His 
            500                 505                 510         


His Glu Leu Ala Ile Ala Ser Lys Ser Asn Val Thr Ile Ile Glu Gly 
        515                 520                 525             


Ser Asp Ser Gly Ile Val Ser Thr Arg Lys Gly Ser Ser Val Ile Ile 
    530                 535                 540                 


Gly Trp Asp Val Ser Ser Thr Arg Arg Ile Val Gln Val Gly Asp Leu 
545                 550                 555                 560 


Arg Val Phe Leu Leu Asp Arg Asn Ser Ala Tyr Asn Tyr Trp Val Pro 
                565                 570                 575     


Glu Leu Pro Thr Glu Gly Thr Ser Pro Gly Phe Ser Thr Ser Lys Thr 
            580                 585                 590         


Thr Ala Ser Ser Ile Ile Val Lys Ala Gly Tyr Leu Leu Arg Gly Ala 
        595                 600                 605             


His Leu Asp Gly Ala Asp Leu His Leu Thr Ala Asp Phe Asn Ala Thr 
    610                 615                 620                 


Thr Pro Ile Glu Val Ile Gly Ala Pro Thr Gly Ala Lys Asn Leu Phe 
625                 630                 635                 640 


Val Asn Gly Glu Lys Ala Ser His Thr Val Asp Lys Asn Gly Ile Trp 
                645                 650                 655     


Ser Ser Glu Val Lys Tyr Ala Ala Pro Glu Ile Lys Leu Pro Gly Leu 
            660                 665                 670         


Lys Asp Leu Asp Trp Lys Tyr Leu Asp Thr Leu Pro Glu Ile Lys Ser 
        675                 680                 685             


Ser Tyr Asp Asp Ser Ala Trp Val Ser Ala Asp Leu Pro Lys Thr Lys 
    690                 695                 700                 


Asn Thr His Arg Pro Leu Asp Thr Pro Thr Ser Leu Tyr Ser Ser Asp 
705                 710                 715                 720 


Tyr Gly Phe His Thr Gly Tyr Leu Ile Tyr Arg Gly His Phe Val Ala 
                725                 730                 735     


Asn Gly Lys Glu Ser Glu Phe Phe Ile Arg Thr Gln Gly Gly Ser Ala 
            740                 745                 750         


Phe Gly Ser Ser Val Trp Leu Asn Glu Thr Tyr Leu Gly Ser Trp Thr 
        755                 760                 765             


Gly Ala Asp Tyr Ala Met Asp Gly Asn Ser Thr Tyr Lys Leu Ser Gln 
    770                 775                 780                 


Leu Glu Ser Gly Lys Asn Tyr Val Ile Thr Val Val Ile Asp Asn Leu 
785                 790                 795                 800 


Gly Leu Asp Glu Asn Trp Thr Val Gly Glu Glu Thr Met Lys Asn Pro 
                805                 810                 815     


Arg Gly Ile Leu Ser Tyr Lys Leu Ser Gly Gln Asp Ala Ser Ala Ile 
            820                 825                 830         


Thr Trp Lys Leu Thr Gly Asn Leu Gly Gly Glu Asp Tyr Gln Asp Lys 
        835                 840                 845             


Val Arg Gly Pro Leu Asn Glu Gly Gly Leu Tyr Ala Glu Arg Gln Gly 
    850                 855                 860                 


Phe His Gln Pro Gln Pro Pro Ser Glu Ser Trp Glu Ser Gly Ser Pro 
865                 870                 875                 880 


Leu Glu Gly Leu Ser Lys Pro Gly Ile Gly Phe Tyr Thr Ala Gln Phe 
                885                 890                 895     


Asp Leu Asp Leu Pro Lys Gly Trp Asp Val Pro Leu Tyr Phe Asn Phe 
            900                 905                 910         


Gly Asn Asn Thr Gln Ala Ala Arg Ala Gln Leu Tyr Val Asn Gly Tyr 
        915                 920                 925             


Gln Tyr Gly Lys Phe Thr Gly Asn Val Gly Pro Gln Thr Ser Phe Pro 
    930                 935                 940                 


Val Pro Glu Gly Ile Leu Asn Tyr Arg Gly Thr Asn Tyr Val Ala Leu 
945                 950                 955                 960 


Ser Leu Trp Ala Leu Glu Ser Asp Gly Ala Lys Leu Gly Ser Phe Glu 
                965                 970                 975     


Leu Ser Tyr Thr Thr Pro Val Leu Thr Gly Tyr Gly Asn Val Glu Ser 
            980                 985                 990         


Pro Glu Gln Pro Lys Tyr Glu Gln  Arg Lys Gly Ala 
        995                 1000                 


<210>  5
<211>  2975
<212>  DNA
<213>  Aspergillus oryzae


<220>
<221>  Galactosidase
<222>  (1)..(2975)

<400>  5
atgaagctcc tctctgttgc tgctgttgcc ttgctggcgg cacaggcagc gggtgcttcc       60

atcaagcacg tctcaatggc ttcacgatcc tggaacatcc ggatccggcg aaaagagact      120

tgctgcaaga cattgttcat gggatgacaa atctctgttc atcaatggag agaggattat      180

gttattcagc ggagaagtgc atccttcaga ttgccagtac cttcgctttg gcttgatatc      240

ttccacaaga tcagagctct tggtttcaac tgtgttcttt ctatattgat tgggctcttc      300

tggagggaaa gcctggcgac tacagagcag aaggcatctt tgcttggaac ccttctttga      360

tgcagccaag gaagcaggca tttatctgat cgcccgcccc ggttcgtaca tcatgccgag      420

gtctcaggcg gtggcttccc tggatggttg cagagggtca atggcactct tcgctcgtct      480

gagagccatt ccttaaagct actgataact atatcgccaa tgccgctgct gccgtggcga      540

aggctcaaat ccgaatggag ggccagtaat tctctaccag cccgaaaacg aatacagcgg      600

tggctgctgc ggtgtcaaat ccccgatgca gactacatgc agtatgttat ggatcaggcc      660

cggaaggctg acattgttgt acctttcata gcaacgatgc ctcaccttct gggcacaatg      720

ctcctggaag tggaacgagc gctgttgata tttatggtac gatagctatc ccctcggctt      780

tgattgcgca aacccatccg tatggcccga gggtaaactg cccgacactt ccgcacgctc      840

catcttgagc agagcccatc aactccgtat tcacttcttg agttccaagc gggtgcttcg      900

acccatgggg tggacccggc tttgaaaaat gctatgccct cgttaaccac gaattctcga      960

gagtttctat aggaacgact tgagtttcgg agtttctacc tttaacttat acatgacttt     1020

cggcggaaca aactgggtaa cctcggacat cccggtggat atacatccta cgactacgga     1080

tcgcctataa ctgaaacgcg aaagttacgc gggagaagta cagcgacata aagctccttg     1140

ccaactttgt caaagcatcg ccatcctatc tcccgctact cccagaaacc tgactactgg     1200

tgtttacaca gacacatctg acctggctgt caccccgtta atggtgatag tccaggctca     1260

ttcttcgtgg tcagacatac ggactattcc agccaagagt caacctcgta aaacttaagc     1320

ttcctaccag tgctggtaac ctgactattc cccagctgga gggcactcta agtctcaacg     1380

acgtgactca aaaattcatg ttgttgatta taatgtgtct ggaacgaaca ttatctattc     1440

gacagctgag tcttcacctg gaagaagttt gacggtaaca aggtcctggt gttatacggc     1500

ggaccgaagg aacaccagaa ttggccattg cctccaagtc aaatgtgacc atcatcgaag     1560

gttcggactc tggaattgtc tcaacgggaa gggcagctct gttatcattg gctgggatgt     1620

ctcttctact cgtcgcatcg ttcaagtcgg tgactgagag tgttcctgct tgataggaac     1680

tctgcttaca actactgggt ccccgaactc cccacagaag gtactctccc gggttcagca     1740

cttcgaagac gaccgcctcc tccattattg tgaaggctgg ctacctcctc cgagcgctca     1800

ccttgatggt gctgatcttc atcttactgc tgatttcaat gccaccaccc cgattgaagt     1860

gacggtgctc caacaggcgc taagaatctg ttcgtgaatg gtgaaaaggc tagccacaca     1920

gtcgacaaga aggcatctgg agcagtgagg tcaagtacgc ggctccagag atcaagctcc     1980

ccggtttgaa ggatttggac ggaagtatct ggacacgctt cccgaaatta agtcttccta     2040

tgatgactcg gcctgggttt cggcagacct ccaaagacaa agaacactca ccgtcctctt     2100

gacacaccaa catcgctata ctcctctgac tatggcttca cactggctac ctgatctaca     2160

ggggtcactt cgttgccaac ggcaaggaaa gcgaattttt tattcgccac aaggcggtag     2220

cgcattcgga agttccgtat ggctgaacga gacgtatctg ggctcttgga ctggtgcgat     2280

tatgcgatgg acggtaactc tacctacaag ctatctcagc tggagtcggg caagaattac     2340

gtcatactgt ggttattgat aacctgggtc tcgacgagaa ttggacggtc ggcgaggaaa     2400

ccatgaagaa tcctgtggta ttcttagcta caagctgagc ggacaagacg ccagcgcaat     2460

cacctggaag ctcactggta acccggagga gaagactacc aggataaggt tagaggacct     2520

ctcaacgaag gtggactgta cgcagagcgc caggcttcca tcagcctcag cctccaagcg     2580

aatcctggga gtcgggcagt ccccttgaag gcctgtcgaa gcgggtatcg gattctacac     2640

tgcccagttc gaccttgacc tcccgaaggg ctgggatgtg ccgctgtact caactttggc     2700

aacaacaccc aggcggctcg ggcccagctc tacgtcaacg gttaccagta tggcaagtta     2760

ctggaaacgt tgggccacag accagcttcc ctgttcccga aggtatcctg aactaccgcg     2820

gaaccaacat gtggcactga gtctttgggc attggagtcg gacggtgcta agctgggtag     2880

cttcgaactg tcctacacac cccagtgctg accggatacg ggaatgttga gtcacctgag     2940

cagcccaagt atgagcagcg gaaggggcat actaa                                2975


