                         SEQUENCE LISTING

<110>  Silence Therapeutics GmbH
 
<120>  siRNAs with at least two ligands at different ends

<130>  S118PCT

<150>  EP18165986.3
<151>  2018-04-05

<150>  GB1815836.0
<151>  2018-09-28

<150>  GB1815925.1
<151>  2018-09-28

<150>  GB1811084.1
<151>  2018-07-05

<150>  GB1811074.2
<151>  2018-07-05

<150>  EP18165917.8
<151>  2018-04-05

<160>  96    

<170>  PatentIn version 3.5

<210>  1
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  1
aaccagaaga agcagguga                                                    19


<210>  2
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  2
ucaccugcuu cuucugguu                                                    19


<210>  3
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  3
tggacaccaa atcgtactgg aa                                                22


<210>  4
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  4
tggacaccaa atcgtactgg aa                                                22


<210>  5
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe - modified as per summary sequence table at the end of the 
       description

<400>  5
acttggcatt tccccgttcc atgaatt                                           27


<210>  6
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  6
caccgccaaa tttaactgca ga                                                22


<210>  7
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  7
aagggtttga taagttctag ctgt                                              24


<210>  8
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe - modified as per summary sequence table at the end of the 
       description

<400>  8
tgcacagtat ccttttgaag accataaccc a                                      31


<210>  9
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  9
ggcaagcctt atgtcatctc gt                                                22


<210>  10
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  10
ggaatggttt tcccatggta ctt                                               23


<210>  11
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe - modified as per summary sequence table at the end of the 
       description

<400>  11
tgaaatgtct ccgctattac gctggctg                                          28


<210>  12
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  12
cggcacctac cttccactct t                                                 21


<210>  13
<211>  17
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  13
tcggtggtgg gcatcct                                                      17


<210>  14
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe - modified as per summary sequence table at the end of the 
       description

<400>  14
ccgagatgtt tccagctccc ctgttcta                                          28


<210>  15
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  15
gtgtcctcgc aacgtcca                                                     18


<210>  16
<211>  15
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  16
gaccccgggg ctttg                                                        15


<210>  17
<211>  27
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe - modified as per summary sequence table at the end of the 
       description

<400>  17
tggctgtttc tgaacaagca ccaatgg                                           27


<210>  18
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  18
gcatgggtca gaaggattcc tat                                               23


<210>  19
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer

<400>  19
tgtagaaggt gtggtgccag att                                               23


<210>  20
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  probe - modified as per summary sequence table at the end of the 
       description

<400>  20
tcgagcacgg catcgtcacc aa                                                22


<210>  21
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  21
uuauagagca agaacacugu u                                                 21


<210>  22
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  22
aacaguguuc uugcucuaua a                                                 21


<210>  23
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  23
uuauagagca agaacacugu u                                                 21


<210>  24
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  24
aacaguguuc uugcucuaua a                                                 21


<210>  25
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  25
uuauagagca agaacacugu u                                                 21


<210>  26
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  26
aacaguguuc uugcucuaua a                                                 21


<210>  27
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  27
uuauagagca agaacacugu u                                                 21


<210>  28
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  28
aacaguguuc uugcucuaua a                                                 21


<210>  29
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  29
uuauagagca agaacacugu u                                                 21


<210>  30
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  30
aacaguguuc uugcucuaua a                                                 21


<210>  31
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  31
uuauagagca agaacacugu u                                                 21


<210>  32
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  32
aacaguguuc uugcucuaua a                                                 21


<210>  33
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  33
uuauagagca agaacacugu u                                                 21


<210>  34
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  34
aacaguguuc uugcucuaua a                                                 21


<210>  35
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  35
uuauagagca agaacacugu u                                                 21


<210>  36
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  36
aacaguguuc uugcucuaua a                                                 21


<210>  37
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  37
uuauagagca agaacacugu u                                                 21


<210>  38
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  38
aacaguguuc uugcucuaua a                                                 21


<210>  39
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  39
uuauagagca agaacacugu u                                                 21


<210>  40
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  40
aacaguguuc uugcucuaua a                                                 21


<210>  41
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  41
uuauagagca agaacacugu u                                                 21


<210>  42
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  42
aacaguguuc uugcucuaua a                                                 21


<210>  43
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  43
uuauagagca agaacacugu u                                                 21


<210>  44
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  44
aacaguguuc uugcucuaua a                                                 21


<210>  45
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  45
ucuucuuaaa cugaguuuc                                                    19


<210>  46
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  46
gaaacucagu uuaagaaga                                                    19


<210>  47
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  47
auguagccga ggaucuucu                                                    19


<210>  48
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  48
agaagauccu cggcuacau                                                    19


<210>  49
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  49
aaccagaaga agcagguga                                                    19


<210>  50
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  50
ucaccugcuu cuucugguu                                                    19


<210>  51
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  51
auaacucugu ccauuaccg                                                    19


<210>  52
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  52
cgguaaugga cagaguuau                                                    19


<210>  53
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  53
uuauagagca agaacacugu u                                                 21


<210>  54
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  54
aacaguguuc uugcucuaua a                                                 21


<210>  55
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  55
uuauagagca agaacacugu u                                                 21


<210>  56
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  56
aacaguguuc uugcucuaua a                                                 21


<210>  57
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  57
uuauagagca agaacacugu u                                                 21


<210>  58
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  58
aacaguguuc uugcucuaua a                                                 21


<210>  59
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  59
ucuucuuaaa cugaguuuc                                                    19


<210>  60
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  60
gaaacucagu uuaagaaga                                                    19


<210>  61
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  61
aaccagaaga agcagguga                                                    19


<210>  62
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  62
ucaccugcuu cuucugguu                                                    19


<210>  63
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  63
uuauagagca agaacacugu u                                                 21


<210>  64
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  64
aacaguguuc uugcucuaua a                                                 21


<210>  65
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  65
uuauagagca agaacacugu u                                                 21


<210>  66
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  66
aacaguguuc uugcucuaua a                                                 21


<210>  67
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  67
ucgaaguauu ccgcguacg                                                    19


<210>  68
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  68
cguacgcgga auacuucga                                                    19


<210>  69
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  69
uuauagagca agaacacugu u                                                 21


<210>  70
<211>  21
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  70
aacaguguuc uugcucuaua a                                                 21


<210>  71
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  71
cuuacucucg cccaagcga                                                    19


<210>  72
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  72
ucgcuugggc gagaguaag                                                    19


<210>  73
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  73
ucuucuuaaa cugaguuuc                                                    19


<210>  74
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  74
gaaacucagu uuaagaaga                                                    19


<210>  75
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  75
auguagccga ggaucuucu                                                    19


<210>  76
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  76
agaagauccu cggcuacau                                                    19


<210>  77
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  77
uaccagaaga agcagguga                                                    19


<210>  78
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  78
ucaccugcuu cuucuggua                                                    19


<210>  79
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  79
aaccagaaga agcagguga                                                    19


<210>  80
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  80
ucaccugcuu cuucugguu                                                    19


<210>  81
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  81
ucgaaguauu ccgcguacg                                                    19


<210>  82
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  82
cguacgcgga auacuucga                                                    19


<210>  83
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  83
uuauagagca agaacacug                                                    19


<210>  84
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  84
caguguucuu gcucuauaa                                                    19


<210>  85
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  85
uuauagagca agaacacug                                                    19


<210>  86
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  86
caguguucuu gcucuauaa                                                    19


<210>  87
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  87
uuauagagca agaacacug                                                    19


<210>  88
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  88
caguguucuu gcucuauaa                                                    19


<210>  89
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  89
aaccagaaga agcagguga                                                    19


<210>  90
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  90
ucaccugcuu cuucugguu                                                    19


<210>  91
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  91
aaccagaaga agcagguga                                                    19


<210>  92
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  92
ucaccugcuu cuucugguu                                                    19


<210>  93
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  93
ucuucuuaaa cugaguuuc                                                    19


<210>  94
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  94
gaaacucagu uuaagaaga                                                    19


<210>  95
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  95
ucuucuuaaa cugaguuuc                                                    19


<210>  96
<211>  19
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  siRNA strand - modified_nucleotides, modified as per summary 
       sequence table at the end of the description

<400>  96
gaaacucagu uuaagaaga                                                    19


