                         SEQUENCE LISTING

<110>  Tsinghua University
       ZHU, Ting
       LIU, Xianyu
       JIANG, Wenjun
       WANG, Min
 
<120>  METHODS OF SEQUENCING AND PRODUCING NUCLEIC ACID SEQUENCES

<130>  76995

<150>  US 62/652,915
<151>  2018-04-05

<160>  36    

<170>  PatentIn version 3.5

<210>  1
<211>  12
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-fluorescein amidite (FAM) labeled 12-nucleotides (nt) L-DNA 
       oligonucleotide (oligo)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM conjugated

<400>  1
actacgaacg cg                                                           12


<210>  2
<211>  11
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-FAM labeled 11-nt L-DNA oligo


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM conjugated

<400>  2
cgcgctgtta t                                                            11


<210>  3
<211>  25
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-FAM labeled 25-nucleotides (nt) L-DNA oligonucleotide (oligo)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM conjugated

<400>  3
atgcctggca gttccctact ctcgc                                             25


<210>  4
<211>  55
<212>  DNA
<213>  Artificial sequence

<220>
<223>  5'-FAM labeled 55-nt L-DNA aptamer


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM conjugated

<400>  4
tcacgtgcat gatagacggc gaagccgtcg agttgctgtg tgccgatgca cgtga            55


<210>  5
<211>  46
<212>  RNA
<213>  Artificial sequence

<220>
<223>  5' FAM-labeled 46-nt L-ribozyme RNA


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM cunjugated

<400>  5
ggaucgaaag auuuccgcau ccccgaaagg guacauggcg uuaggu                      46


<210>  6
<211>  352
<212>  PRT
<213>  Sulfolobus solfataricus

<400>  6

Met Ile Val Leu Phe Val Asp Phe Asp Tyr Phe Tyr Ala Gln Val Glu 
1               5                   10                  15      


Glu Val Leu Asn Pro Ser Leu Lys Gly Lys Pro Val Val Val Cys Val 
            20                  25                  30          


Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala Val Ala Thr Ala Asn Tyr 
        35                  40                  45              


Glu Ala Arg Lys Phe Gly Val Lys Ala Gly Ile Pro Ile Val Glu Ala 
    50                  55                  60                  


Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu Pro Met Arg Lys Glu Val 
65                  70                  75                  80  


Tyr Gln Gln Val Ser Ser Arg Ile Met Asn Leu Leu Arg Glu Tyr Ser 
                85                  90                  95      


Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu Ala Tyr Leu Asp Ile Ser 
            100                 105                 110         


Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr Asn Leu Gly Leu Glu Ile 
        115                 120                 125             


Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile Thr Val Thr Val Gly Ile 
    130                 135                 140                 


Ser Lys Asn Lys Val Phe Ala Lys Ile Ala Ala Asp Met Ala Lys Pro 
145                 150                 155                 160 


Asn Gly Ile Lys Val Ile Asp Asp Glu Glu Val Lys Arg Leu Ile Arg 
                165                 170                 175     


Glu Leu Asp Ile Ala Asp Val Pro Gly Ile Gly Asn Ile Thr Ala Glu 
            180                 185                 190         


Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu Val Asp Thr Leu Ser Ile 
        195                 200                 205             


Glu Phe Asp Lys Leu Lys Gly Met Ile Gly Glu Ala Lys Ala Lys Tyr 
    210                 215                 220                 


Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn Glu Pro Ile Arg Thr Arg 
225                 230                 235                 240 


Val Arg Lys Ser Ile Gly Arg Ile Val Thr Met Lys Arg Asn Ser Arg 
                245                 250                 255     


Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe Arg Ala Ile Glu Glu Ser 
            260                 265                 270         


Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys Ala Ile His Val Val Ala 
        275                 280                 285             


Val Thr Glu Asp Leu Asp Ile Val Ser Arg Gly Arg Thr Phe Pro His 
    290                 295                 300                 


Gly Ile Ser Lys Glu Thr Ala Tyr Ser Glu Ser Val Lys Leu Leu Gln 
305                 310                 315                 320 


Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile Arg Arg Ile Gly Val Arg 
                325                 330                 335     


Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu Asp Lys Phe Phe Asp Thr 
            340                 345                 350         


<210>  7
<211>  416
<212>  PRT
<213>  Sulfolobus solfataricus

<400>  7

Met Ile Val Leu Phe Val Asp Phe Asp Tyr Phe Tyr Ala Gln Val Glu 
1               5                   10                  15      


Glu Val Leu Asn Pro Ser Leu Lys Gly Lys Pro Val Val Val Cys Val 
            20                  25                  30          


Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala Val Ala Thr Ala Asn Tyr 
        35                  40                  45              


Glu Ala Arg Lys Phe Gly Val Lys Ala Gly Ile Pro Ile Val Glu Ala 
    50                  55                  60                  


Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu Pro Met Arg Lys Glu Val 
65                  70                  75                  80  


Tyr Gln Gln Val Ser Ser Arg Ile Met Asn Leu Leu Arg Glu Tyr Ser 
                85                  90                  95      


Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu Ala Tyr Leu Asp Ile Ser 
            100                 105                 110         


Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr Asn Leu Gly Leu Glu Ile 
        115                 120                 125             


Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile Thr Val Thr Val Gly Ile 
    130                 135                 140                 


Ser Lys Asn Lys Val Phe Ala Lys Ile Ala Ala Asp Met Ala Lys Pro 
145                 150                 155                 160 


Asn Gly Ile Lys Val Ile Asp Asp Glu Glu Val Lys Arg Leu Ile Arg 
                165                 170                 175     


Glu Leu Asp Ile Ala Asp Val Pro Gly Ile Gly Asn Ile Thr Ala Glu 
            180                 185                 190         


Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu Val Asp Thr Leu Ser Ile 
        195                 200                 205             


Glu Phe Asp Lys Leu Lys Gly Met Ile Gly Glu Ala Lys Ala Lys Tyr 
    210                 215                 220                 


Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn Glu Pro Ile Arg Thr Arg 
225                 230                 235                 240 


Val Arg Lys Ser Ile Gly Arg Ile Val Thr Met Lys Arg Asn Ser Arg 
                245                 250                 255     


Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe Arg Ala Ile Glu Glu Ser 
            260                 265                 270         


Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys Ala Ile His Val Val Ala 
        275                 280                 285             


Val Thr Glu Asp Leu Asp Ile Val Ser Arg Gly Arg Thr Phe Pro His 
    290                 295                 300                 


Gly Ile Ser Lys Glu Thr Ala Tyr Ser Glu Ser Val Lys Leu Leu Gln 
305                 310                 315                 320 


Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile Arg Arg Ile Gly Val Arg 
                325                 330                 335     


Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu Asp Lys Phe Phe Asp Thr 
            340                 345                 350         


Gln Ala Glu Leu Cys Ala Asp Pro Lys Glu Leu Trp Val Gln Gln Leu 
        355                 360                 365             


Met Gln His Leu Asp Lys Thr Pro Ser Pro Gln Lys Pro Ala Gln Gly 
    370                 375                 380                 


Cys Arg Lys Asp Arg Gly Ala Ser Lys Thr Gly Lys Lys Gly Lys Gly 
385                 390                 395                 400 


Ser Lys Gly Cys Lys Arg Thr Glu Arg Ser Gln Thr Pro Lys Gly Pro 
                405                 410                 415     


<210>  8
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4 wt with 5' His tag

<400>  8

His His His His His His Met Ile Val Leu Phe Val Asp Phe Asp Tyr 
1               5                   10                  15      


Phe Tyr Ala Gln Val Glu Glu Val Leu Asn Pro Ser Leu Lys Gly Lys 
            20                  25                  30          


Pro Val Val Val Cys Val Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala 
        35                  40                  45              


Val Ala Thr Ala Asn Tyr Glu Ala Arg Lys Phe Gly Val Lys Ala Gly 
    50                  55                  60                  


Ile Pro Ile Val Glu Ala Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Met Arg Lys Glu Val Tyr Gln Gln Val Ser Ser Arg Ile Met Asn 
                85                  90                  95      


Leu Leu Arg Glu Tyr Ser Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu 
            100                 105                 110         


Ala Tyr Leu Asp Ile Ser Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr 
        115                 120                 125             


Asn Leu Gly Leu Glu Ile Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile 
    130                 135                 140                 


Thr Val Thr Val Gly Ile Ser Lys Asn Lys Val Phe Ala Lys Ile Ala 
145                 150                 155                 160 


Ala Asp Met Ala Lys Pro Asn Gly Ile Lys Val Ile Asp Asp Glu Glu 
                165                 170                 175     


Val Lys Arg Leu Ile Arg Glu Leu Asp Ile Ala Asp Val Pro Gly Ile 
            180                 185                 190         


Gly Asn Ile Thr Ala Glu Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu 
        195                 200                 205             


Val Asp Thr Leu Ser Ile Glu Phe Asp Lys Leu Lys Gly Met Ile Gly 
    210                 215                 220                 


Glu Ala Lys Ala Lys Tyr Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn 
225                 230                 235                 240 


Glu Pro Ile Arg Thr Arg Val Arg Lys Ser Ile Gly Arg Ile Val Thr 
                245                 250                 255     


Met Lys Arg Asn Ser Arg Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe 
            260                 265                 270         


Arg Ala Ile Glu Glu Ser Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys 
        275                 280                 285             


Ala Ile His Val Val Ala Val Thr Glu Asp Leu Asp Ile Val Ser Arg 
    290                 295                 300                 


Gly Arg Thr Phe Pro His Gly Ile Ser Lys Glu Thr Ala Tyr Ser Glu 
305                 310                 315                 320 


Ser Val Lys Leu Leu Gln Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile 
                325                 330                 335     


Arg Arg Ile Gly Val Arg Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu 
            340                 345                 350         


Asp Lys Phe Phe Asp Thr 
        355             


<210>  9
<211>  352
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4 wt without 5' His tag

<400>  9

Met Ile Val Leu Phe Val Asp Phe Asp Tyr Phe Tyr Ala Gln Val Glu 
1               5                   10                  15      


Glu Val Leu Asn Pro Ser Leu Lys Gly Lys Pro Val Val Val Cys Val 
            20                  25                  30          


Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala Val Ala Thr Ala Asn Tyr 
        35                  40                  45              


Glu Ala Arg Lys Phe Gly Val Lys Ala Gly Ile Pro Ile Val Glu Ala 
    50                  55                  60                  


Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu Pro Met Arg Lys Glu Val 
65                  70                  75                  80  


Tyr Gln Gln Val Ser Ser Arg Ile Met Asn Leu Leu Arg Glu Tyr Ser 
                85                  90                  95      


Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu Ala Tyr Leu Asp Ile Ser 
            100                 105                 110         


Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr Asn Leu Gly Leu Glu Ile 
        115                 120                 125             


Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile Thr Val Thr Val Gly Ile 
    130                 135                 140                 


Ser Lys Asn Lys Val Phe Ala Lys Ile Ala Ala Asp Met Ala Lys Pro 
145                 150                 155                 160 


Asn Gly Ile Lys Val Ile Asp Asp Glu Glu Val Lys Arg Leu Ile Arg 
                165                 170                 175     


Glu Leu Asp Ile Ala Asp Val Pro Gly Ile Gly Asn Ile Thr Ala Glu 
            180                 185                 190         


Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu Val Asp Thr Leu Ser Ile 
        195                 200                 205             


Glu Phe Asp Lys Leu Lys Gly Met Ile Gly Glu Ala Lys Ala Lys Tyr 
    210                 215                 220                 


Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn Glu Pro Ile Arg Thr Arg 
225                 230                 235                 240 


Val Arg Lys Ser Ile Gly Arg Ile Val Thr Met Lys Arg Asn Ser Arg 
                245                 250                 255     


Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe Arg Ala Ile Glu Glu Ser 
            260                 265                 270         


Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys Ala Ile His Val Val Ala 
        275                 280                 285             


Val Thr Glu Asp Leu Asp Ile Val Ser Arg Gly Arg Thr Phe Pro His 
    290                 295                 300                 


Gly Ile Ser Lys Glu Thr Ala Tyr Ser Glu Ser Val Lys Leu Leu Gln 
305                 310                 315                 320 


Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile Arg Arg Ile Gly Val Arg 
                325                 330                 335     


Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu Asp Lys Phe Phe Asp Thr 
            340                 345                 350         


<210>  10
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-5m with 5' His tag


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (95)..(95)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa is isosteric methionine analog norleucine

<400>  10

His His His His His His Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr 
1               5                   10                  15      


Phe Tyr Ala Gln Val Glu Glu Val Leu Asn Pro Ser Leu Lys Gly Lys 
            20                  25                  30          


Pro Val Val Val Ser Val Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala 
        35                  40                  45              


Val Ala Thr Ala Asn Tyr Glu Ala Arg Lys Phe Gly Val Lys Ala Gly 
    50                  55                  60                  


Ile Pro Ile Val Glu Ala Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Xaa Arg Lys Glu Val Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn 
                85                  90                  95      


Leu Leu Arg Glu Tyr Ser Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu 
            100                 105                 110         


Ala Tyr Leu Asp Ile Ser Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr 
        115                 120                 125             


Ala Leu Gly Leu Glu Ile Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile 
    130                 135                 140                 


Thr Val Thr Val Gly Ile Ser Lys Asn Lys Val Phe Ala Lys Ile Ala 
145                 150                 155                 160 


Ala Asp Xaa Ala Lys Pro Asn Gly Ile Lys Val Ile Asp Asp Glu Glu 
                165                 170                 175     


Val Lys Arg Leu Ile Arg Glu Leu Asp Ile Ala Asp Val Pro Gly Ile 
            180                 185                 190         


Gly Asn Ile Thr Ala Glu Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu 
        195                 200                 205             


Val Asp Thr Leu Ala Ile Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly 
    210                 215                 220                 


Glu Ala Lys Ala Lys Tyr Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn 
225                 230                 235                 240 


Glu Pro Ile Arg Thr Arg Val Arg Lys Ser Ile Gly Arg Ile Val Thr 
                245                 250                 255     


Xaa Lys Arg Asn Ser Arg Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe 
            260                 265                 270         


Arg Ala Ile Glu Glu Ser Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys 
        275                 280                 285             


Ala Ile His Val Val Ala Val Thr Glu Asp Leu Asp Ile Val Ser Arg 
    290                 295                 300                 


Gly Arg Thr Phe Pro His Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu 
305                 310                 315                 320 


Ser Val Lys Leu Leu Gln Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile 
                325                 330                 335     


Arg Arg Ile Gly Val Arg Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu 
            340                 345                 350         


Asp Lys Phe Phe Asp Thr 
        355             


<210>  11
<211>  352
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-5m without His tag


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (157)..(157)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (216)..(216)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (251)..(251)
<223>  Xaa is isosteric methionine analog norleucine

<400>  11

Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr Phe Tyr Ala Gln Val Glu 
1               5                   10                  15      


Glu Val Leu Asn Pro Ser Leu Lys Gly Lys Pro Val Val Val Ser Val 
            20                  25                  30          


Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala Val Ala Thr Ala Asn Tyr 
        35                  40                  45              


Glu Ala Arg Lys Phe Gly Val Lys Ala Gly Ile Pro Ile Val Glu Ala 
    50                  55                  60                  


Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu Pro Xaa Arg Lys Glu Val 
65                  70                  75                  80  


Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn Leu Leu Arg Glu Tyr Ser 
                85                  90                  95      


Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu Ala Tyr Leu Asp Ile Ser 
            100                 105                 110         


Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr Ala Leu Gly Leu Glu Ile 
        115                 120                 125             


Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile Thr Val Thr Val Gly Ile 
    130                 135                 140                 


Ser Lys Asn Lys Val Phe Ala Lys Ile Ala Ala Asp Xaa Ala Lys Pro 
145                 150                 155                 160 


Asn Gly Ile Lys Val Ile Asp Asp Glu Glu Val Lys Arg Leu Ile Arg 
                165                 170                 175     


Glu Leu Asp Ile Ala Asp Val Pro Gly Ile Gly Asn Ile Thr Ala Glu 
            180                 185                 190         


Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu Val Asp Thr Leu Ala Ile 
        195                 200                 205             


Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly Glu Ala Lys Ala Lys Tyr 
    210                 215                 220                 


Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn Glu Pro Ile Arg Thr Arg 
225                 230                 235                 240 


Val Arg Lys Ser Ile Gly Arg Ile Val Thr Xaa Lys Arg Asn Ser Arg 
                245                 250                 255     


Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe Arg Ala Ile Glu Glu Ser 
            260                 265                 270         


Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys Ala Ile His Val Val Ala 
        275                 280                 285             


Val Thr Glu Asp Leu Asp Ile Val Ser Arg Gly Arg Thr Phe Pro His 
    290                 295                 300                 


Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu Ser Val Lys Leu Leu Gln 
305                 310                 315                 320 


Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile Arg Arg Ile Gly Val Arg 
                325                 330                 335     


Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu Asp Lys Phe Phe Asp Thr 
            340                 345                 350         


<210>  12
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-6m-Y12S with 5' His tag


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (95)..(95)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa is isosteric methionine analog norleucine

<400>  12

His His His His His His Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr 
1               5                   10                  15      


Phe Ser Ala Gln Val Glu Glu Val Leu Asn Pro Ser Leu Lys Gly Lys 
            20                  25                  30          


Pro Val Val Val Ser Val Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala 
        35                  40                  45              


Val Ala Thr Ala Asn Tyr Glu Ala Arg Lys Phe Gly Val Lys Ala Gly 
    50                  55                  60                  


Ile Pro Ile Val Glu Ala Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Xaa Arg Lys Glu Val Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn 
                85                  90                  95      


Leu Leu Arg Glu Tyr Ser Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu 
            100                 105                 110         


Ala Tyr Leu Asp Ile Ser Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr 
        115                 120                 125             


Ala Leu Gly Leu Glu Ile Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile 
    130                 135                 140                 


Thr Val Thr Val Gly Ile Ser Lys Asn Lys Val Phe Ala Lys Ile Ala 
145                 150                 155                 160 


Ala Asp Xaa Ala Lys Pro Asn Gly Ile Lys Val Ile Asp Asp Glu Glu 
                165                 170                 175     


Val Lys Arg Leu Ile Arg Glu Leu Asp Ile Ala Asp Val Pro Gly Ile 
            180                 185                 190         


Gly Asn Ile Thr Ala Glu Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu 
        195                 200                 205             


Val Asp Thr Leu Ala Ile Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly 
    210                 215                 220                 


Glu Ala Lys Ala Lys Tyr Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn 
225                 230                 235                 240 


Glu Pro Ile Arg Thr Arg Val Arg Lys Ser Ile Gly Arg Ile Val Thr 
                245                 250                 255     


Xaa Lys Arg Asn Ser Arg Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe 
            260                 265                 270         


Arg Ala Ile Glu Glu Ser Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys 
        275                 280                 285             


Ala Ile His Val Val Ala Val Thr Glu Asp Leu Asp Ile Val Ser Arg 
    290                 295                 300                 


Gly Arg Thr Phe Pro His Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu 
305                 310                 315                 320 


Ser Val Lys Leu Leu Gln Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile 
                325                 330                 335     


Arg Arg Ile Gly Val Arg Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu 
            340                 345                 350         


Asp Lys Phe Phe Asp Thr 
        355             


<210>  13
<211>  352
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-6m-Y12S without  His tag


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (157)..(157)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (216)..(216)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (251)..(251)
<223>  Xaa is isosteric methionine analog norleucine

<400>  13

Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr Phe Ser Ala Gln Val Glu 
1               5                   10                  15      


Glu Val Leu Asn Pro Ser Leu Lys Gly Lys Pro Val Val Val Ser Val 
            20                  25                  30          


Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala Val Ala Thr Ala Asn Tyr 
        35                  40                  45              


Glu Ala Arg Lys Phe Gly Val Lys Ala Gly Ile Pro Ile Val Glu Ala 
    50                  55                  60                  


Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu Pro Xaa Arg Lys Glu Val 
65                  70                  75                  80  


Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn Leu Leu Arg Glu Tyr Ser 
                85                  90                  95      


Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu Ala Tyr Leu Asp Ile Ser 
            100                 105                 110         


Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr Ala Leu Gly Leu Glu Ile 
        115                 120                 125             


Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile Thr Val Thr Val Gly Ile 
    130                 135                 140                 


Ser Lys Asn Lys Val Phe Ala Lys Ile Ala Ala Asp Xaa Ala Lys Pro 
145                 150                 155                 160 


Asn Gly Ile Lys Val Ile Asp Asp Glu Glu Val Lys Arg Leu Ile Arg 
                165                 170                 175     


Glu Leu Asp Ile Ala Asp Val Pro Gly Ile Gly Asn Ile Thr Ala Glu 
            180                 185                 190         


Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu Val Asp Thr Leu Ala Ile 
        195                 200                 205             


Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly Glu Ala Lys Ala Lys Tyr 
    210                 215                 220                 


Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn Glu Pro Ile Arg Thr Arg 
225                 230                 235                 240 


Val Arg Lys Ser Ile Gly Arg Ile Val Thr Xaa Lys Arg Asn Ser Arg 
                245                 250                 255     


Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe Arg Ala Ile Glu Glu Ser 
            260                 265                 270         


Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys Ala Ile His Val Val Ala 
        275                 280                 285             


Val Thr Glu Asp Leu Asp Ile Val Ser Arg Gly Arg Thr Phe Pro His 
    290                 295                 300                 


Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu Ser Val Lys Leu Leu Gln 
305                 310                 315                 320 


Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile Arg Arg Ile Gly Val Arg 
                325                 330                 335     


Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu Asp Lys Phe Phe Asp Thr 
            340                 345                 350         


<210>  14
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-6m-Y12A with 5' His tag


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (95)..(95)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa is isosteric methionine analog norleucine

<400>  14

His His His His His His Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr 
1               5                   10                  15      


Phe Ala Ala Gln Val Glu Glu Val Leu Asn Pro Ser Leu Lys Gly Lys 
            20                  25                  30          


Pro Val Val Val Ser Val Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala 
        35                  40                  45              


Val Ala Thr Ala Asn Tyr Glu Ala Arg Lys Phe Gly Val Lys Ala Gly 
    50                  55                  60                  


Ile Pro Ile Val Glu Ala Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Xaa Arg Lys Glu Val Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn 
                85                  90                  95      


Leu Leu Arg Glu Tyr Ser Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu 
            100                 105                 110         


Ala Tyr Leu Asp Ile Ser Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr 
        115                 120                 125             


Ala Leu Gly Leu Glu Ile Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile 
    130                 135                 140                 


Thr Val Thr Val Gly Ile Ser Lys Asn Lys Val Phe Ala Lys Ile Ala 
145                 150                 155                 160 


Ala Asp Xaa Ala Lys Pro Asn Gly Ile Lys Val Ile Asp Asp Glu Glu 
                165                 170                 175     


Val Lys Arg Leu Ile Arg Glu Leu Asp Ile Ala Asp Val Pro Gly Ile 
            180                 185                 190         


Gly Asn Ile Thr Ala Glu Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu 
        195                 200                 205             


Val Asp Thr Leu Ala Ile Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly 
    210                 215                 220                 


Glu Ala Lys Ala Lys Tyr Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn 
225                 230                 235                 240 


Glu Pro Ile Arg Thr Arg Val Arg Lys Ser Ile Gly Arg Ile Val Thr 
                245                 250                 255     


Xaa Lys Arg Asn Ser Arg Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe 
            260                 265                 270         


Arg Ala Ile Glu Glu Ser Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys 
        275                 280                 285             


Ala Ile His Val Val Ala Val Thr Glu Asp Leu Asp Ile Val Ser Arg 
    290                 295                 300                 


Gly Arg Thr Phe Pro His Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu 
305                 310                 315                 320 


Ser Val Lys Leu Leu Gln Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile 
                325                 330                 335     


Arg Arg Ile Gly Val Arg Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu 
            340                 345                 350         


Asp Lys Phe Phe Asp Thr 
        355             


<210>  15
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-6m-Y12A without  His tag


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (95)..(95)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa is isosteric methionine analog norleucine

<400>  15

His His His His His His Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr 
1               5                   10                  15      


Phe Ala Ala Gln Val Glu Glu Val Leu Asn Pro Ser Leu Lys Gly Lys 
            20                  25                  30          


Pro Val Val Val Ser Val Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala 
        35                  40                  45              


Val Ala Thr Ala Asn Tyr Glu Ala Arg Lys Phe Gly Val Lys Ala Gly 
    50                  55                  60                  


Ile Pro Ile Val Glu Ala Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Xaa Arg Lys Glu Val Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn 
                85                  90                  95      


Leu Leu Arg Glu Tyr Ser Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu 
            100                 105                 110         


Ala Tyr Leu Asp Ile Ser Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr 
        115                 120                 125             


Ala Leu Gly Leu Glu Ile Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile 
    130                 135                 140                 


Thr Val Thr Val Gly Ile Ser Lys Asn Lys Val Phe Ala Lys Ile Ala 
145                 150                 155                 160 


Ala Asp Xaa Ala Lys Pro Asn Gly Ile Lys Val Ile Asp Asp Glu Glu 
                165                 170                 175     


Val Lys Arg Leu Ile Arg Glu Leu Asp Ile Ala Asp Val Pro Gly Ile 
            180                 185                 190         


Gly Asn Ile Thr Ala Glu Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu 
        195                 200                 205             


Val Asp Thr Leu Ala Ile Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly 
    210                 215                 220                 


Glu Ala Lys Ala Lys Tyr Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn 
225                 230                 235                 240 


Glu Pro Ile Arg Thr Arg Val Arg Lys Ser Ile Gly Arg Ile Val Thr 
                245                 250                 255     


Xaa Lys Arg Asn Ser Arg Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe 
            260                 265                 270         


Arg Ala Ile Glu Glu Ser Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys 
        275                 280                 285             


Ala Ile His Val Val Ala Val Thr Glu Asp Leu Asp Ile Val Ser Arg 
    290                 295                 300                 


Gly Arg Thr Phe Pro His Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu 
305                 310                 315                 320 


Ser Val Lys Leu Leu Gln Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile 
                325                 330                 335     


Arg Arg Ile Gly Val Arg Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu 
            340                 345                 350         


Asp Lys Phe Phe Asp Thr 
        355             


<210>  16
<211>  358
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-6m-Y12G with 5' His tag


<220>
<221>  misc_feature
<222>  (7)..(7)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (82)..(82)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (95)..(95)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (163)..(163)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (222)..(222)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (257)..(257)
<223>  Xaa is isosteric methionine analog norleucine

<400>  16

His His His His His His Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr 
1               5                   10                  15      


Phe Gly Ala Gln Val Glu Glu Val Leu Asn Pro Ser Leu Lys Gly Lys 
            20                  25                  30          


Pro Val Val Val Ser Val Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala 
        35                  40                  45              


Val Ala Thr Ala Asn Tyr Glu Ala Arg Lys Phe Gly Val Lys Ala Gly 
    50                  55                  60                  


Ile Pro Ile Val Glu Ala Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu 
65                  70                  75                  80  


Pro Xaa Arg Lys Glu Val Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn 
                85                  90                  95      


Leu Leu Arg Glu Tyr Ser Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu 
            100                 105                 110         


Ala Tyr Leu Asp Ile Ser Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr 
        115                 120                 125             


Ala Leu Gly Leu Glu Ile Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile 
    130                 135                 140                 


Thr Val Thr Val Gly Ile Ser Lys Asn Lys Val Phe Ala Lys Ile Ala 
145                 150                 155                 160 


Ala Asp Xaa Ala Lys Pro Asn Gly Ile Lys Val Ile Asp Asp Glu Glu 
                165                 170                 175     


Val Lys Arg Leu Ile Arg Glu Leu Asp Ile Ala Asp Val Pro Gly Ile 
            180                 185                 190         


Gly Asn Ile Thr Ala Glu Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu 
        195                 200                 205             


Val Asp Thr Leu Ala Ile Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly 
    210                 215                 220                 


Glu Ala Lys Ala Lys Tyr Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn 
225                 230                 235                 240 


Glu Pro Ile Arg Thr Arg Val Arg Lys Ser Ile Gly Arg Ile Val Thr 
                245                 250                 255     


Xaa Lys Arg Asn Ser Arg Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe 
            260                 265                 270         


Arg Ala Ile Glu Glu Ser Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys 
        275                 280                 285             


Ala Ile His Val Val Ala Val Thr Glu Asp Leu Asp Ile Val Ser Arg 
    290                 295                 300                 


Gly Arg Thr Phe Pro His Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu 
305                 310                 315                 320 


Ser Val Lys Leu Leu Gln Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile 
                325                 330                 335     


Arg Arg Ile Gly Val Arg Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu 
            340                 345                 350         


Asp Lys Phe Phe Asp Thr 
        355             


<210>  17
<211>  352
<212>  PRT
<213>  Artificial sequence

<220>
<223>  Dpo4-6m-Y12G without His tag


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (76)..(76)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (89)..(89)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (157)..(157)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (216)..(216)
<223>  Xaa is isosteric methionine analog norleucine

<220>
<221>  misc_feature
<222>  (251)..(251)
<223>  Xaa is isosteric methionine analog norleucine

<400>  17

Xaa Ile Val Leu Phe Val Asp Phe Asp Tyr Phe Gly Ala Gln Val Glu 
1               5                   10                  15      


Glu Val Leu Asn Pro Ser Leu Lys Gly Lys Pro Val Val Val Ser Val 
            20                  25                  30          


Phe Ser Gly Arg Phe Glu Asp Ser Gly Ala Val Ala Thr Ala Asn Tyr 
        35                  40                  45              


Glu Ala Arg Lys Phe Gly Val Lys Ala Gly Ile Pro Ile Val Glu Ala 
    50                  55                  60                  


Lys Lys Ile Leu Pro Asn Ala Val Tyr Leu Pro Xaa Arg Lys Glu Val 
65                  70                  75                  80  


Tyr Gln Gln Val Ser Cys Arg Ile Xaa Asn Leu Leu Arg Glu Tyr Ser 
                85                  90                  95      


Glu Lys Ile Glu Ile Ala Ser Ile Asp Glu Ala Tyr Leu Asp Ile Ser 
            100                 105                 110         


Asp Lys Val Arg Asp Tyr Arg Glu Ala Tyr Ala Leu Gly Leu Glu Ile 
        115                 120                 125             


Lys Asn Lys Ile Leu Glu Lys Glu Lys Ile Thr Val Thr Val Gly Ile 
    130                 135                 140                 


Ser Lys Asn Lys Val Phe Ala Lys Ile Ala Ala Asp Xaa Ala Lys Pro 
145                 150                 155                 160 


Asn Gly Ile Lys Val Ile Asp Asp Glu Glu Val Lys Arg Leu Ile Arg 
                165                 170                 175     


Glu Leu Asp Ile Ala Asp Val Pro Gly Ile Gly Asn Ile Thr Ala Glu 
            180                 185                 190         


Lys Leu Lys Lys Leu Gly Ile Asn Lys Leu Val Asp Thr Leu Ala Ile 
        195                 200                 205             


Glu Phe Asp Lys Leu Lys Gly Xaa Ile Gly Glu Ala Lys Ala Lys Tyr 
    210                 215                 220                 


Leu Ile Ser Leu Ala Arg Asp Glu Tyr Asn Glu Pro Ile Arg Thr Arg 
225                 230                 235                 240 


Val Arg Lys Ser Ile Gly Arg Ile Val Thr Xaa Lys Arg Asn Ser Arg 
                245                 250                 255     


Asn Leu Glu Glu Ile Lys Pro Tyr Leu Phe Arg Ala Ile Glu Glu Ser 
            260                 265                 270         


Tyr Tyr Lys Leu Asp Lys Arg Ile Pro Lys Ala Ile His Val Val Ala 
        275                 280                 285             


Val Thr Glu Asp Leu Asp Ile Val Ser Arg Gly Arg Thr Phe Pro His 
    290                 295                 300                 


Gly Ile Ser Lys Glu Thr Ala Tyr Ala Glu Ser Val Lys Leu Leu Gln 
305                 310                 315                 320 


Lys Ile Leu Glu Glu Asp Glu Arg Lys Ile Arg Arg Ile Gly Val Arg 
                325                 330                 335     


Phe Ser Lys Phe Ile Glu Ala Ile Gly Leu Asp Lys Phe Phe Asp Thr 
            340                 345                 350         


<210>  18
<211>  41
<212>  DNA
<213>  Artificial sequence

<220>
<223>  (D)-41-nt DNA template

<400>  18
ggacggcatt ggatcgacga tgagttggtt ggacggctgc g                           41


<210>  19
<211>  41
<212>  RNA
<213>  Artificial sequence

<220>
<223>  (D)-41-nt RNA template

<400>  19
ggacggcauu ggaucgacga ugaguugguu ggacggcugc g                           41


<210>  20
<211>  21
<212>  DNA
<213>  Artificial sequence

<220>
<223>  (D)- 21 nt DNA primer (DNA-1-P)  5' FAM labelled


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM conjugated

<400>  20
cgcagccgtc caaccaactc a                                                 21


<210>  21
<211>  21
<212>  RNA
<213>  Artificial sequence

<220>
<223>  (D)- 21 nt RNA primer (RNA-1-P)  5' FAM labelled


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM conjugated

<400>  21
cgcagccguc caaccaacuc a                                                 21


<210>  22
<211>  15
<212>  DNA
<213>  Artificial sequence

<220>
<223>  (L)-15-nt DNA primer  5' FAM labelled


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5' FAM conjugated

<400>  22
acctaacgcc atgta                                                        15


<210>  23
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L)-FAM-PCR-R-DNA primer 5' FAM labelled

<400>  23
atgcctggca gttccctact ctcgc                                             25


<210>  24
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L)-PCR-F-DNA primer 5' FAM labelled

<400>  24
tgcctggcgg cagtagcgc                                                    19


<210>  25
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L)-120-nt DNA template

<400>  25
atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc       60

gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca      120


<210>  26
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L)-120-nt 5S RNA template (a transcription product of SEQ ID NO:
       25


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  26
ugccuggcgg caguagcgcg guggucccac cugaccccau gccgaacuca gaagugaaac       60

gccguagcgc cgaugguagu guggggucuc cccaugcgag aguagggaac ugccaggcau      120


<210>  27
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L) 25 nt DNA Primer (for RT of L-5S rRNA)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-Cy5

<400>  27
atgcctggca gttccctact ctcgc                                             25


<210>  28
<211>  120
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (D)-120-nt DNA (marker)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-Cy5

<400>  28
atgcctggca gttccctact ctcgcatggg gagaccccac actaccatcg gcgctacggc       60

gtttcacttc tgagttcggc atggggtcag gtgggaccac cgcgctactg ccgccaggca      120


<210>  29
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L) 5S rRNA-PCR-F primer

<400>  29
tgcctggcgg cagtagcgc                                                    19


<210>  30
<211>  25
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L) 5S rRNA-PCR-R primer

<400>  30
atgcctggca gttccctact ctcgc                                             25


<210>  31
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L) 76 nt tRNA template

<400>  31
gggucguuag cucaguuggu agagcaguug acuuuuaauc aauuggucgc agguucgaau       60

ccugcacgac ccacca                                                       76


<210>  32
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L)-FAM-14 nt DNA primer (for RT of L-76 nt tRNA)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  32
tggtgggtcg tgca                                                         14


<210>  33
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L) 18 nt PCR-F primer (for amplifying 76 nt RT DNA)

<400>  33
gggtcgttag ctcagttg                                                     18


<210>  34
<211>  14
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L) 14 nt PCR-R primer (for amplifying 76 nt RT DNA)

<400>  34
tggtgggtcg tgca                                                         14


<210>  35
<211>  18
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L) 18 nt PCR-F primer (for amplifying 76 nt RT DNA)


<220>
<221>  misc_feature
<222>  (1)..(1)
<223>  5'-FAM

<400>  35
gggtcgttag ctcagttg                                                     18


<210>  36
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (L)- 76 nt DNA (a reverse transcription product of SEQ ID NO: 31)

<400>  36
tggtgggtcg tgcaggattc gaacctgcga ccaattgatt aaaagtcaac tgctctacca       60

actgagctaa cgaccc                                                       76


