                         SEQUENCE LISTING

<110>  KWS SAAT SE
 
<120>  Plants with improved digestibility and marker haplotypes

<130>  KWS0277PCT

<150>  EP18169122.1
<151>  2018-04-24

<160>  40    

<170>  PatentIn version 3.5

<210>  1
<211>  4215
<212>  DNA
<213>  Zea mays

<400>  1
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  2
<211>  1584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of SEQ ID NO: 1

<400>  2
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acagatacaa ggacatgatc      660

gtgtcgctgc tgaccggcac gggcatgttc aacatcagcg acttcgtgcc ggcgctggcg      720

cgtctggacc tgcagggcgt gcaggcgaag ctgcggcgcg tccaccgcca gttcgacggc      780

ctcatcacca agctgctggc cgagcacgcc gcgacggccg cggaccgcgc gcgccagggc      840

cgcccggact tcgtcgaccg gctccgcgcc acgatggacg ccggcgccgc cgccgacgac      900

gagagcggcg agaccatcac cgaggtcaac atcaagggcc tcatcttcga catgttcacg      960

gcgggtacgg acacgtcgtc gatcatcgtg gagtgggcga tggcggagat gctcaagaac     1020

ccgaccgtca tggcgcgcgc gcaggaggag ctggaccgcg cggtgggccg gggccggcgc     1080

ctggaggagt cggacctgcc cggcctcccc tacctgcagg cggtgtgcaa ggaggccatg     1140

cggctgcacc cgtccacgcc gctcagcctc ccgcacttct ccttggacgc ctgcgacgac     1200

gtcgacggct accgcgtccc ggccaacacc cgcctgctcg tcaacgtctg ggccatcggc     1260

cgggacccgg aggcctggga gaggcccctc gacttccgcc ccgagcgctt cctgcccggg     1320

ggcggcgcgg agaaggtcga ccccctgggg aactgcttcg agctcatccc gttcggcgcc     1380

ggccggagga tctgcgcggg gaagctggcg ggcatggtgt tcgtgcagta cttcctgggc     1440

acgctgctgc acgcgttcga ctggcgcctg cctgacggcg aggagaagct ggacatgagc     1500

gagacgttcg gcctcgcgct gcccaaggca gtgccgctcc gcgccgtcgc cacgccacgg     1560

ctcgtgccgg aagcctatgc ctga                                            1584


<210>  3
<211>  527
<212>  PRT
<213>  Zea mays

<400>  3

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  4
<211>  5903
<212>  DNA
<213>  Sorghum bicolor

<400>  4
cccctacgat gtatatgtag ctcgtctgaa aacggttaaa taaaaaaaaa agataacaac       60

ggtcccgaga tttcgagtac acagcgcctc atcaccaata taaaaaccgt cgtgctcgcg      120

gaggcatcct tagcttgggc aggtcctcct ccctagctag ctagcacagt aacgccacca      180

tgaagctcgc cgcgttgtgc accgaccccg tcgtgctctc ctgcgccttc ctctgcctcc      240

tcctccacct cgctctccgg tccctgctgc acccttcttc tgccgcctct tcctctggcc      300

gccgcgccgg cgccggcggc cacctcccgc cggggccacc gggcctgccg atcctcggcg      360

cgctgccact cgtcggccct gccccgcatg ccggcctggc cgcgctggcg cgcaagtacg      420

gtcccatcat gtacctgaag atgggcacga cgggcgtcgt ggtggcgtcg tccccgtgcg      480

cggcgaagac gttcctcaag gcgctggacg cgaagtacgc caaccggccg gcggtggcga      540

gcgccgccga catcacgtac cggcgccaga acatggtgtt cgcggactac gggcccaagt      600

ggaagctgat gcggaagctc gccagcgtgc acctgctcgg ggcgcgcgcg ctcgccgact      660

gggcgtgcgt gcggcgcgac gaggccggcc acctgctccg cggcgtggcg gaggcggccg      720

cggcccggcg gcccgtcgtc gtgccggagc tgctcgtgtg cgcgctcgcc aacatcgtcg      780

ggcagatcac cgtcagcaag cgggtgttcg acgcgcaggg ggatgactca aacaggtgag      840

gatcgatcaa agctgcatga aatcctatca acaagacaat gaccatatat atataaggca      900

taaaatccaa gttcatttcg aaaggaaagc acatgttttt cttccaaaat ttcatgatta      960

tcgggtgcct taagttggga tgaggtttac aatatctttg aatgggacta gtgagatcag     1020

ttgaactagt tatccttaat taatgcaatg cttgacggaa tgaacatcag cagaccgtta     1080

atgcggaaag ccggggataa aaaaattaaa aagttcagct aggaagctgc tgtcatcggg     1140

tacgtatgag aagactgaag aatcggcatg acgagcactt agtactggca tttagccgga     1200

atgtccttgt tagtgcagag agtcgctcac caaaacctga aagcgaaaag agagcgatcg     1260

aaacggcccc tccatcgaca aataataagg agaatcttgt catttttcat gcatgcattc     1320

atggaaaaaa cacaaactga tcaagtattt ttcgtctaaa catataataa ttctgatacc     1380

atatatgcgt gctgcgtgct gcgtgctgcg tgcaggtaca aggacatgat cgtgtcgctg     1440

ctgacgggca cgggcatgtt caacatcagc gacttcgtgc cggcgctggc gcgtctggac     1500

ctgcagggcg tgcaggccaa gctgcggcgg atccaccacc agttcgacgg cctcatcacc     1560

aagctgctgg ccgagcacgc cgcgacggcg gcggaccgcg cgcgccaggg ccgccaggac     1620

ttcgtcgaca ggctccgcgc cacgatggac gccggcgccg acgacgagag cggcgagacc     1680

atcaccgagg tcaacatcaa gggcctcatc ttcgtaagct ctcgctctct ttatattttc     1740

ctcaccccca acctctcttc atgcatttat attttaattg cttgcttccc ttggttgcta     1800

actaaccaac tactaatctg tcgtagctgc aaaaatatgt aatactactg caacaatcga     1860

tcatggccat gggtaactga atcatatact ccctccatcc caaattgtaa gtcattccaa     1920

gaattttgaa gagtcaaact tttcaaagtt tgaccaaatt tatatgataa aataataatt     1980

attatgatac caactaaata tcattagatt cttccttaat tatattttca tagtatactc     2040

atcttacgtt acaaatctta gtatttcact ctataatttt ggtcaaacgt gaaaatgctt     2100

tgactctcca agattcttgg aatgacttac aatttgagat ggagggagta tatcttcgct     2160

gaaaatgaag tgcacggcat tttggtgtga cataggagct aagctaagct aagctagtag     2220

ccaccggacc taacggagca ctgacactga ccgaaagctc agcgtgttag gtgaagctgc     2280

tagtggagtt gttggaaaga caatatcaaa atcacaatct cgaactcatg agatgatgtg     2340

gggcctgcag ggccggtttt gagtcctcca tgatggtgat agctcaaggc ttgtttggtt     2400

caaacattac tagagcaaaa tatggttcaa acattaattg ctaaccgttg aagagtagaa     2460

tgcatatata tgcctgtcat gccatggttt tatgagaaga tgcttactat atatttttat     2520

tagataacaa tagggatcac tagatttaat tatctgccga gagaacatac agatttaatc     2580

caacctaaat tcacccccgt ctccttgttc tcacggtgga aatatatagt gggtaaactg     2640

acaaaaaagg catttggttc ctatactttg ttggaacata tttttaattt gttaggctat     2700

atagcataca gatccaacaa attccaccca taaaatatct atcaatacgc ataaaagtag     2760

tttaaggaaa tctagaaata ttggaaccac tactagctag aatctgtatg ttttcctagg     2820

attttaattc ctacgaatag aactaaaata tgtaaggaaa acatttccct atcatatttt     2880

gcaccaaaaa aaattctatc gatgcataaa tagtaaaaca cgaaaaatgt tgggacaata     2940

gggacttatt taccccaagg gaaccaaagc acctaaaaat tatacatttc ctgaaggaaa     3000

tttagatagt agttccaaga aaaactagaa aagtggtggc atagatatgc tagggaattc     3060

attccctaag ggaatgagaa tgtatgaaaa ttatacaatt tcaaataagc tctaaagata     3120

aagaggtatt ctcttttctg gaacaaacaa aatatgaaag tgcctccaaa gatcttattg     3180

agtcaacttt tatagaatta tatattgttt ttattatttt taagatggcg tgcaaaatca     3240

agtctaaatt attcagtgaa agcctcgaga ttctttaagg attaaaaata agtgaagcgg     3300

ttaagattta atcaggaaaa cgatattgga ctcctgtcct ccaaccaaac ttgattatga     3360

cgttttagct taatgttcac tttgtagacg tcggatatat agggatgcga accgaaagga     3420

aaaggctgac gttttaacaa actgcacata tatgttacca gaactcgaat agcagctttg     3480

taagtctcta gaatgttgga gtagttaaca acgtgataga cgatgtaaca cctggaccat     3540

gttattatgt catttcctag aagaattgta atcaaatcta ttaattaatc aagcaaagaa     3600

ttgaaagcta taaatggctt aaggcaacat ctggttgtga gttgtgtgat taattattgg     3660

tgaatgtcgt gtcagcacta acattattaa ggtcagtctc aatgcatagt ttcatgacac     3720

agttaccaag actataaact aggtaaccga gccacaagag tttcatgggg atgaaactcc     3780

tctctcatct gatgaaactc cttcatttaa tgactctgcc aactcagcaa ttttgcttat     3840

gtggcaccat atttaatgtg catgacactc tcataaaaca tgcattgaga ctggcctaag     3900

ggggtgttta gtgttggact ctttagatcg tctactaggt cgtagatcgg tggggaattt     3960

acttctattt gggataaacc acatagaatt tcatggatct agagaggaat ggaggacgag     4020

aggatgtgtt atatactgaa ccataccttt ggggttggcg gagaagcgcg tggtcgaggc     4080

agtggcggtg gcggactttc cgtcgctggc agtgcctccc tctcgatcgg tctagggtta     4140

gatggggact cgcggcattg tagcggtgaa ccttgtaact tgtgctctag cctccaccct     4200

ctcttttata cactgcgcga caggggcctg gcagccaagt tattgggcta gacgcccccg     4260

atcagggcgt ggtcaagggt ccggccaagt ctttggactt gggccagtgg agatcaatct     4320

aacatttagt tccccataaa atacaaaatg tcaactactt tggaatgatg aaatgcaaaa     4380

tatcaaaaat ttcaggttag atccatccaa aatgcagaat ttattgctct cattatagcc     4440

tcttgagact tttttggact tttttggcct cttgaggcca aaatacaaaa tagagaataa     4500

ccacattttt gttaaaattt ttgcatagta tttattttta ttatgtaaaa taatacacca     4560

aaactcagaa ttttttggga tggaagggga actaaacacc ccgtgaatct agtcggcaca     4620

atgactcaag caatgaaacc actactgttt cagtataagg ttggaaaaca ctgttctcaa     4680

cataacatcg cattgactct ctctttgtgg gctgtgtggc tttgtgggtc agcttgattg     4740

gagctcctta acatatataa catacactgt gcctgctgat gctgaggttg tactgtcatc     4800

aaacacacta aaacagtgca ccacaccctt caataatgtc aggtcaaact atataaccag     4860

cttatattat atacagtgtt aaacttaaac ccaacccaac gatatataat tcacttgcac     4920

atacttcaaa accatgtata tatatctcgt tgaaaaaaat tataggagta ataatgtttg     4980

cgtgcgacgc atcgctgctt tgcatgcacg caggacatgt tcacggcggg cacggacacg     5040

tcgtccatca tcgtggagtg ggcgatggcg gagatgctca agaacccgtc cgtgatggcg     5100

cgcgcgcagg aggagctgga ccgcgtggtg ggccgcggcc gccgcctgga ggagtcggac     5160

ctgcccagcc tcccctacct gcaggccgtc tgcaaggagg ccatgcggct gcacccgtcg     5220

acgccgctca gcctcccgca cttctccttc gacgcctgcg acgacgacgt cgccgccggc     5280

ggctaccgcg tcccggccaa cacccggctg ctcatcaaca tctgggccat cggccgggac     5340

ccggcggcgt ggaagaagcc cctggagttc cggcccgagc ggttcctgcc gggcggcggc     5400

gccgagaagg tcgacccgat ggggaactgc ttcgagctca tcccgttcgg cgccggcagg     5460

aggatctgcg ccgggaagct ggccggcatg gtgttcgtgc agtacttcct gggcacgctg     5520

ctgcacgcgt tcgactggag cttgcctgac ggcgaggaga agctggacat gagcgagacg     5580

ttcggcctcg cgctgcctaa ggctgtgccg ctccgcgccg tcgtcacgcc acggctcgtg     5640

ccggaagcct acgcctgagc tagctgtcag tggttcgcgt gtgttgttgt tgccttcctg     5700

catacgtaca taacttgtta tgaatcctac acttgcattg gttgcctagt accagtattt     5760

gaaattatat acggaccttg catggtttca gttttttgac acaaccgatg atggttgatc     5820

agaagacgga tctgtagagt tgttctttaa caatgcgcta acctatatat caagggactg     5880

tttggttttc catgactaaa att                                             5903


<210>  5
<211>  1599
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of SEQ ID NO: 4

<400>  5
atgaagctcg ccgcgttgtg caccgacccc gtcgtgctct cctgcgcctt cctctgcctc       60

ctcctccacc tcgctctccg gtccctgctg cacccttctt ctgccgcctc ttcctctggc      120

cgccgcgccg gcgccggcgg ccacctcccg ccggggccac cgggcctgcc gatcctcggc      180

gcgctgccac tcgtcggccc tgccccgcat gccggcctgg ccgcgctggc gcgcaagtac      240

ggtcccatca tgtacctgaa gatgggcacg acgggcgtcg tggtggcgtc gtccccgtgc      300

gcggcgaaga cgttcctcaa ggcgctggac gcgaagtacg ccaaccggcc ggcggtggcg      360

agcgccgccg acatcacgta ccggcgccag aacatggtgt tcgcggacta cgggcccaag      420

tggaagctga tgcggaagct cgccagcgtg cacctgctcg gggcgcgcgc gctcgccgac      480

tgggcgtgcg tgcggcgcga cgaggccggc cacctgctcc gcggcgtggc ggaggcggcc      540

gcggcccggc ggcccgtcgt cgtgccggag ctgctcgtgt gcgcgctcgc caacatcgtc      600

gggcagatca ccgtcagcaa gcgggtgttc gacgcgcagg gggatgactc aaacaggtac      660

aaggacatga tcgtgtcgct gctgacgggc acgggcatgt tcaacatcag cgacttcgtg      720

ccggcgctgg cgcgtctgga cctgcagggc gtgcaggcca agctgcggcg gatccaccac      780

cagttcgacg gcctcatcac caagctgctg gccgagcacg ccgcgacggc ggcggaccgc      840

gcgcgccagg gccgccagga cttcgtcgac aggctccgcg ccacgatgga cgccggcgcc      900

gacgacgaga gcggcgagac catcaccgag gtcaacatca agggcctcat cttcgacatg      960

ttcacggcgg gcacggacac gtcgtccatc atcgtggagt gggcgatggc ggagatgctc     1020

aagaacccgt ccgtgatggc gcgcgcgcag gaggagctgg accgcgtggt gggccgcggc     1080

cgccgcctgg aggagtcgga cctgcccagc ctcccctacc tgcaggccgt ctgcaaggag     1140

gccatgcggc tgcacccgtc gacgccgctc agcctcccgc acttctcctt cgacgcctgc     1200

gacgacgacg tcgccgccgg cggctaccgc gtcccggcca acacccggct gctcatcaac     1260

atctgggcca tcggccggga cccggcggcg tggaagaagc ccctggagtt ccggcccgag     1320

cggttcctgc cgggcggcgg cgccgagaag gtcgacccga tggggaactg cttcgagctc     1380

atcccgttcg gcgccggcag gaggatctgc gccgggaagc tggccggcat ggtgttcgtg     1440

cagtacttcc tgggcacgct gctgcacgcg ttcgactgga gcttgcctga cggcgaggag     1500

aagctggaca tgagcgagac gttcggcctc gcgctgccta aggctgtgcc gctccgcgcc     1560

gtcgtcacgc cacggctcgt gccggaagcc tacgcctag                            1599


<210>  6
<211>  532
<212>  PRT
<213>  Sorghum bicolor

<400>  6

Met Lys Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Ser Cys Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Leu Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Ser Ser Ala Ala Ser Ser Ser Gly Arg Arg Ala Gly Ala Gly Gly His 
        35                  40                  45              


Leu Pro Pro Gly Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu 
    50                  55                  60                  


Val Gly Pro Ala Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr 
65                  70                  75                  80  


Gly Pro Ile Met Tyr Leu Lys Met Gly Thr Thr Gly Val Val Val Ala 
                85                  90                  95      


Ser Ser Pro Cys Ala Ala Lys Thr Phe Leu Lys Ala Leu Asp Ala Lys 
            100                 105                 110         


Tyr Ala Asn Arg Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Arg 
        115                 120                 125             


Arg Gln Asn Met Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met 
    130                 135                 140                 


Arg Lys Leu Ala Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp 
145                 150                 155                 160 


Trp Ala Cys Val Arg Arg Asp Glu Ala Gly His Leu Leu Arg Gly Val 
                165                 170                 175     


Ala Glu Ala Ala Ala Ala Arg Arg Pro Val Val Val Pro Glu Leu Leu 
            180                 185                 190         


Val Cys Ala Leu Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg 
        195                 200                 205             


Val Phe Asp Ala Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile 
    210                 215                 220                 


Val Ser Leu Leu Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val 
225                 230                 235                 240 


Pro Ala Leu Ala Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg 
                245                 250                 255     


Arg Ile His His Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu 
            260                 265                 270         


His Ala Ala Thr Ala Ala Asp Arg Ala Arg Gln Gly Arg Gln Asp Phe 
        275                 280                 285             


Val Asp Arg Leu Arg Ala Thr Met Asp Ala Gly Ala Asp Asp Glu Ser 
    290                 295                 300                 


Gly Glu Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met 
305                 310                 315                 320 


Phe Thr Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met 
                325                 330                 335     


Ala Glu Met Leu Lys Asn Pro Ser Val Met Ala Arg Ala Gln Glu Glu 
            340                 345                 350         


Leu Asp Arg Val Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu 
        355                 360                 365             


Pro Ser Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu 
    370                 375                 380                 


His Pro Ser Thr Pro Leu Ser Leu Pro His Phe Ser Phe Asp Ala Cys 
385                 390                 395                 400 


Asp Asp Asp Val Ala Ala Gly Gly Tyr Arg Val Pro Ala Asn Thr Arg 
                405                 410                 415     


Leu Leu Ile Asn Ile Trp Ala Ile Gly Arg Asp Pro Ala Ala Trp Lys 
            420                 425                 430         


Lys Pro Leu Glu Phe Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala 
        435                 440                 445             


Glu Lys Val Asp Pro Met Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly 
    450                 455                 460                 


Ala Gly Arg Arg Ile Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val 
465                 470                 475                 480 


Gln Tyr Phe Leu Gly Thr Leu Leu His Ala Phe Asp Trp Ser Leu Pro 
                485                 490                 495     


Asp Gly Glu Glu Lys Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu 
            500                 505                 510         


Pro Lys Ala Val Pro Leu Arg Ala Val Val Thr Pro Arg Leu Val Pro 
        515                 520                 525             


Glu Ala Tyr Ala 
    530         


<210>  7
<211>  3752
<212>  DNA
<213>  Sorghum bicolor

<400>  7
atatagcttg tctgagttcc gagtacgcag ccgcaggagc gcagcgtgct gcagggtgca       60

acgcctcatc aggaatataa agccgtcgtc caggagacac ccttagcttg ggaaggtcct      120

cctccctggc acagcacagt agcacgccac catgcagctc gccgcgttgt gcatcgaccc      180

cctcgtgctc tcctgcgcct tcctctgcct cctcctccac gtcgcgctcc gctccctgct      240

gcacccttct tctgcctcgt cctccggccg ccgcggcggc caccaccagc tcccgccggg      300

gccaccggga ctaccgatcc tcggcgcgct gccactcgtc ggcccagccc cgcacgccgg      360

cctggccgcg ctggcgcgca agtacggtcc catcatgtac ctgaagatgg gcacgacggg      420

cgtcgtggtg gcgtcgtccc cgggcgcggc gaggacgttc ctcaaggcgc tggacgcgaa      480

gtacgccaac cggccggcgg tggcgagcgc cgccgacatc acgtacgggt gccagaacat      540

ggtgttcgcc aactacgggc ccaagtggaa gctgatgcgg aagctcgcca gcgtgcacct      600

gctcggggcg cgcgcgctcg cggactgggc gcacgtgcgg cgcgacgagg ccggccacct      660

gctccgcggc gtggcggagg cggccgcggc ccggcggccc gtcgtcgtgc cggaggtgct      720

ggtgtgcgcc ctcgccaata tcgtcgggca gatcaccgtg agtaagcggg tgttcgacgc      780

acagggggac gagtccaaca ggtcagaact gaaccgaaag gccttttcag tgaatagaat      840

cgctcaccaa aatacgaatg tgaaatatgt gtgtgcaggt acaaggacat gatcgtgtca      900

ctgctgactg gcgcgggact gttcaacatc agcgacttcg tgccggcgct ggcgcgtctg      960

gacctgcagg gcgtgcaggc caagctgcgg cgtgtgcacc accagttcga cggcctcatc     1020

accaagctgc tggccgagca cgccgcgacg gccgcggacc gcgcgcgcga cggccgccag     1080

gacttcgtcg acaggctccg cgccgccatt gacgccggcg ctgacgacga gagcggcgag     1140

accatcaccg aggtcaacat caagggcctc atcttcgtaa gctcgctttc ctcacctcag     1200

ctacatacat atatactctt tccttccttc cattgctttc gttgttttat caagtaaagt     1260

aatgcattcc gtcgcggtaa gaatgtaaga tatgcagcag ctagtaccga tcatgaaaga     1320

aagtaactga gaatcttgtg ctcaaaataa ggcattgttt agttccgaaa aaaatttacc     1380

aaaaatttta gccgttaaat taaatcttgc ggcatatata tggagtatta aagatataaa     1440

aataaaaatt aattacatag tttgcctgta atttatgaga cgaaccttta aacctagtta     1500

gtttataatt ggacaatatt tgtcaaataa aaataaaaat gctacagtat ctgaaattcc     1560

aaaattttgt caactgaaca aggccttaga tgcatttagg ccatgtttgg tttcaaatgt     1620

taaagtttat cgctcgacac atcgaatgtt tagtacacat gcatagagta ctaaatatag     1680

actatttatg aaactaaaaa cacagctaga gagtaatttg cgagatgaat cttttaaacc     1740

taattaatct atgattagac attaattgtt aaataagatg aaaatgttac aacccaacca     1800

agcaccccct tacgtcgtcg cgttggtgcg gccgagcagg agagttaggt ggactagtgg     1860

gttgttggaa agtgacgatc gaagcgaaag cacacattag ctcgagctga tatgtttggt     1920

ttatggtcta attcataccg tgagggcctg caggttctga gtctttcatg attagtcaag     1980

gtgatatcgg tagctgatgg ctttgtttgg taccaaagtg cagactagag caaaatatgg     2040

ttcaaatgtt gataacattt ggtaccgtga agtgaataga atacctaccg gttgtgccat     2100

ggttgtcctc aaaattcatg tttttttaat accccatcta ttttaaatta caagacattt     2160

tgacttttct agataaattg atttttctat gtatctagat atagtgtcta tctaaatgta     2220

tagtaaagat tacatatatt taaaaaagct aaacatttta taattagaat agggggagta     2280

tataattaaa agagagtatc tgtttatagt gatatttctc aagtacagtc cacatacaga     2340

atgcaccgag accattctaa aacctcaaac atgttgcaaa gatctaatgt tctggaacca     2400

aaatatatga actgaatatc gacgattgaa tcattgggta caacaaatat tgtagatttc     2460

aggatctatg accattgatc catcaagttg gacgcttgac ttctccgcag ctaccaatgg     2520

tgacgtcaat gacctggagt taatatctct acggagtatt actcgtcctt gcacaaggat     2580

gaccttatcg tggccatgaa aatagacata tagacaatgt agggtttatc tagaataatc     2640

actaataaga ataagatgag attgaataac atccaaacag tgtcggatct actcatttgg     2700

ttaattggta actttaccca caaaacactg aagtaattcg tgtccattta attttttccg     2760

tcctatacaa tgtttgcatg caacgcatcc aggacatgtt cacggcgggc acggacacat     2820

cgtcgatcat cgtggagtgg gcgatgtctg aaatgctcaa gaacccgccc gtcatggcac     2880

gagcgcagga ggagctggac cgcacggtcg gccgcggccg ccgcctggaa gagtcggacc     2940

tgcccagcct cccctacctg caggccgtct gcaaggaggc catgcggctg cacccgtcga     3000

cgccgctcag cctcccgcac ttctccttcg acgcctgcga cgacgtcggc ggcggctacc     3060

gcgtcccggc caacacccgg ctgctcatca acgtctgggc catcggccgg gacccggcga     3120

cgtgggaggc gcccctcgag ttccggccag agcggttcct gccgggggcc gcggcggaga     3180

aggtcgaccc gatggggaac tacttcgagc tcatcccctt cggcgccggc cggaggatct     3240

gcgccgggaa gctggccggc atggtgttcg tgcagtactt cctgggcacg ctgctgcacg     3300

cgttcaactg gcgcctcgcc gacggcgagg agctggacat gcgcgagacg ttcgggctca     3360

cgctgcctaa ggccgtaccg ctccgcgcca ttgtcacgcc gcggctcctg ccggaagcct     3420

atgcttgagt tagttgtcac ttgtcagtct cagtccgcgc tgtggaagga tgatcggaag     3480

gtttgtgctt gccaacctgc atacatattc tgtttatatg tttttttata gaaactacat     3540

ggtattcatc atatacacaa acgcacatct ctctatgctt aattgcaagg catttgatat     3600

ggaaaatcca ccttggattc aatagtttga caactactga tcttactgaa caaatgatta     3660

ttccggtcca ttaaaatgaa cttttacagg cagttttgta agaccgttca tgtgcttcaa     3720

cttcaagcag taaggcctca tttggttgca tc                                   3752


<210>  8
<211>  1584
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  cDNA of SEQ ID NO: 7

<400>  8
atgcagctcg ccgcgttgtg catcgacccc ctcgtgctct cctgcgcctt cctctgcctc       60

ctcctccacg tcgcgctccg ctccctgctg cacccttctt ctgcctcgtc ctccggccgc      120

cgcggcggcc accaccagct cccgccgggg ccaccgggac taccgatcct cggcgcgctg      180

ccactcgtcg gcccagcccc gcacgccggc ctggccgcgc tggcgcgcaa gtacggtccc      240

atcatgtacc tgaagatggg cacgacgggc gtcgtggtgg cgtcgtcccc gggcgcggcg      300

aggacgttcc tcaaggcgct ggacgcgaag tacgccaacc ggccggcggt ggcgagcgcc      360

gccgacatca cgtacgggtg ccagaacatg gtgttcgcca actacgggcc caagtggaag      420

ctgatgcgga agctcgccag cgtgcacctg ctcggggcgc gcgcgctcgc ggactgggcg      480

cacgtgcggc gcgacgaggc cggccacctg ctccgcggcg tggcggaggc ggccgcggcc      540

cggcggcccg tcgtcgtgcc ggaggtgctg gtgtgcgccc tcgccaatat cgtcgggcag      600

atcaccgtga gtaagcgggt gttcgacgca cagggggacg agtccaacag gtacaaggac      660

atgatcgtgt cactgctgac tggcgcggga ctgttcaaca tcagcgactt cgtgccggcg      720

ctggcgcgtc tggacctgca gggcgtgcag gccaagctgc ggcgtgtgca ccaccagttc      780

gacggcctca tcaccaagct gctggccgag cacgccgcga cggccgcgga ccgcgcgcgc      840

gacggccgcc aggacttcgt cgacaggctc cgcgccgcca ttgacgccgg cgctgacgac      900

gagagcggcg agaccatcac cgaggtcaac atcaagggcc tcatcttcga catgttcacg      960

gcgggcacgg acacatcgtc gatcatcgtg gagtgggcga tgtctgaaat gctcaagaac     1020

ccgcccgtca tggcacgagc gcaggaggag ctggaccgca cggtcggccg cggccgccgc     1080

ctggaagagt cggacctgcc cagcctcccc tacctgcagg ccgtctgcaa ggaggccatg     1140

cggctgcacc cgtcgacgcc gctcagcctc ccgcacttct ccttcgacgc ctgcgacgac     1200

gtcggcggcg gctaccgcgt cccggccaac acccggctgc tcatcaacgt ctgggccatc     1260

ggccgggacc cggcgacgtg ggaggcgccc ctcgagttcc ggccagagcg gttcctgccg     1320

ggggccgcgg cggagaaggt cgacccgatg gggaactact tcgagctcat ccccttcggc     1380

gccggccgga ggatctgcgc cgggaagctg gccggcatgg tgttcgtgca gtacttcctg     1440

ggcacgctgc tgcacgcgtt caactggcgc ctcgccgacg gcgaggagct ggacatgcgc     1500

gagacgttcg ggctcacgct gcctaaggcc gtaccgctcc gcgccattgt cacgccgcgg     1560

ctcctgccgg aagcctatgc ttga                                            1584


<210>  9
<211>  527
<212>  PRT
<213>  Sorghum bicolor

<400>  9

Met Gln Leu Ala Ala Leu Cys Ile Asp Pro Leu Val Leu Ser Cys Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Ser Ser Ala Ser Ser Ser Gly Arg Arg Gly Gly His His Gln Leu Pro 
        35                  40                  45              


Pro Gly Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly 
    50                  55                  60                  


Pro Ala Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro 
65                  70                  75                  80  


Ile Met Tyr Leu Lys Met Gly Thr Thr Gly Val Val Val Ala Ser Ser 
                85                  90                  95      


Pro Gly Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Lys Tyr Ala 
            100                 105                 110         


Asn Arg Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Cys Gln 
        115                 120                 125             


Asn Met Val Phe Ala Asn Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys 
    130                 135                 140                 


Leu Ala Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala 
145                 150                 155                 160 


His Val Arg Arg Asp Glu Ala Gly His Leu Leu Arg Gly Val Ala Glu 
                165                 170                 175     


Ala Ala Ala Ala Arg Arg Pro Val Val Val Pro Glu Val Leu Val Cys 
            180                 185                 190         


Ala Leu Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe 
        195                 200                 205             


Asp Ala Gln Gly Asp Glu Ser Asn Arg Tyr Lys Asp Met Ile Val Ser 
    210                 215                 220                 


Leu Leu Thr Gly Ala Gly Leu Phe Asn Ile Ser Asp Phe Val Pro Ala 
225                 230                 235                 240 


Leu Ala Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val 
                245                 250                 255     


His His Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala 
            260                 265                 270         


Ala Thr Ala Ala Asp Arg Ala Arg Asp Gly Arg Gln Asp Phe Val Asp 
        275                 280                 285             


Arg Leu Arg Ala Ala Ile Asp Ala Gly Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ser Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Pro Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Thr Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Ser 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Phe Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Gly Gly Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Ile Asn 
                405                 410                 415     


Val Trp Ala Ile Gly Arg Asp Pro Ala Thr Trp Glu Ala Pro Leu Glu 
            420                 425                 430         


Phe Arg Pro Glu Arg Phe Leu Pro Gly Ala Ala Ala Glu Lys Val Asp 
        435                 440                 445             


Pro Met Gly Asn Tyr Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg 
    450                 455                 460                 


Ile Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu 
465                 470                 475                 480 


Gly Thr Leu Leu His Ala Phe Asn Trp Arg Leu Ala Asp Gly Glu Glu 
                485                 490                 495     


Leu Asp Met Arg Glu Thr Phe Gly Leu Thr Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Ile Val Thr Pro Arg Leu Leu Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  10
<211>  187
<212>  DNA
<213>  Zea mays

<400>  10
cttctgccca gaagcgggcc cagacatttg agattgggta ttcaaaaatt caaaagatta       60

aagaatttag tgttctaacg ctattttatg caatacatta ttgacaaatt agtgttctaa      120

cactatagat caccaaaaac atgggtattc aatgaatacc catgaaaccc ccctgggccc      180

gcccatg                                                                187


<210>  11
<211>  4402
<212>  DNA
<213>  Zea mays

<400>  11
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgcccagaa gcgggcccag      120

acatttgaga ttgggtattc aaaaattcaa aagattaaag aatttagtgt tctaacgcta      180

ttttatgcaa tacattattg acaaattagt gttctaacac tatagatcac caaaaacatg      240

ggtattcaat gaatacccat gaaacccccc tgggcccgcc catgcttctg ccgcctcttc      300

ctccgggcgc cgcgggcagc tcccgccggg gccaccgggc ctgccgatcc tcggcgcgct      360

gccactcgtg ggcccagccc cgcacgccgg cctggccgcg ctggcgcgca agtacggtcc      420

catcatgtac ctgaagatgg gcacggccgg cgtggtggtg gcgtcgtccc cgcgcgcggc      480

gcggacgttc ctcaaggcgc tggacgcgcg gtacgccaac cggccggccg tggcgagcgc      540

cgcggacatc acgtacgggc ggcagaacat ggtgttcgcg gactacgggc ccaagtggaa      600

gctgatgcgg aagctcgcca gcgtgcacct gctcggcgcg cgcgcgctcg cggactgggc      660

gtgcgtgcgg cgcggcgagg ccggccacgt gctgcgcggc gtggcggagg cggccgcggc      720

cggcaggccc gtcgtcgtgc cggagctgct cgtgtgcgcc ctcgccaaca tcgtcgggca      780

gatcacagtg agcaagcggg tgttcgacgc gcagggggac gactcgaaca ggtgaggatg      840

ggaggtccat gaaatcctac cagctgtgag catgcataaa agttcatttg gaaagaaaag      900

aacatatttt tcttacaaat ttatgcttac tgtttcttta agtttcgata aagtttgtaa      960

aaaaaattta ggctagtttg aaactccatt taggatttct attttccaaa gaaaaataaa     1020

cgaatttctc ttgaaaaaat gaaaattctt tagaaaaata ggttctcaaa ctagccctca     1080

ataaaactta atgcgatcgt tttctctgac tctcattcat ctttctctgg ttatctaatt     1140

gggtccttga gagatgagtt tacctgcttg tcctttatta ttgcaaagac aacatatctg     1200

atgcacatgg aacattggtg cacatggtgc acatatgaaa tcatcaccac tcattttaaa     1260

tctaacgtct atagttgttt gatatatttt attaaggaca ccctccaacg tggtggtgtg     1320

tagtggtgga aggtgttatt tgtaaattga ataatcaact agagacgtta gatctaaaat     1380

gagtggtgat gatttaatat gtgcaccatg tgcaccagtc ttctatgtgc accagatatg     1440

tcctctattg caaatgctag acggaacacc agctagcact agcagactgt ttatgtggaa     1500

agaaaaaact taaaaagatc agctaggaag ctgctgtcat ctgtacgtat atatggtgaa     1560

gactgaacaa tctgcatgac aagcaaaact tagcttaaaa gcgaaaagag cgatggaaac     1620

ggccgctcga taaataatta atgagagtct tgggattttt catgcatgga aaaaaacaaa     1680

gctggcattt ttcatctaat ataatatata acgctgatat catattgcgt gcagatacaa     1740

ggacatgatc gtgtcgctgc tgaccggcac gggcatgttc aacatcagcg acttcgtgcc     1800

ggcgctggcg cgtctggacc tgcagggcgt gcaggcgaag ctgcggcgcg tccaccgcca     1860

gttcgacggc ctcatcacca agctgctggc cgagcacgcc gcgacggccg cggaccgcgc     1920

gcgccagggc cgcccggact tcgtcgaccg gctccgcgcc acgatggacg ccggcgccgc     1980

cgccgacgac gagagcggcg agaccatcac cgaggtcaac atcaagggcc tcatcttcgt     2040

aagctccctg ctttttcctc gcccccaacc atgcatcatc atatgcactt atattttaca     2100

cttgctcggt tttcctttag taactaacta atccgtcgca gctgcgatac acgtagcact     2160

agtactacag cgatgggtca tcggtaactg aatctaaggt gcaatagagt gcacggccgc     2220

gggatcatgg cgtgacatgg gagctaagct aagccagtgg ccacctaacg aaggcactga     2280

ccgaaagctc agtggcgtgt taggtggaga tagtggatcg agttgttgga aagacaatat     2340

caaaaccact ctccaattga tgatgtgtag ggcctgcagt gttttgaatc caccttgttt     2400

ggtcgaacac attactagag tgaaatatgg ttccaatgtt aattgatagc gcgaaagggt     2460

ctctagcgta atggttaaac cttccgagta gcacatccag gttgggttcg atcctctcga     2520

gggcgaattt tcaagctttg ttaaaaaaat tatctcgttg tgccccgtcc gctctcagga     2580

atcgatattc tacacgacac cctccgacta gtgacagttg attgactcgt tagtgatgag     2640

aagccatgct aaaaaagtgg agacgtagat atgatagagg ttccctttcc taagcaaacg     2700

tgaatgctat gaaaattatg cagtttaaaa aaaactttaa agataaacag gaattctctt     2760

ttttggaaca aacaatacga atgcacctcc aaatatctta tcgagtcgac ttttatggaa     2820

ttattgtttt tgttatttct aagatgggag cccaaaatca catacaaatt attcagtgaa     2880

tgcctcggtg tttttttatt agttaagggc tctcattttt ttcaagggat ttttattttt     2940

ttccaaaaga aaataaacta atcctcttta gaaaaatgga aatctattgg agaaatgagg     3000

ttcctaaact agctctaaca gtgagtcagt taatcaggag aagatattag actcctgtat     3060

agtgtgcagc aaccacatcc gattctgacg ttttagctta atgttcgcta tgtagacgtc     3120

gggcataggg aatgcattgc taccagaaca cgaatgacag ctatgcaagt ctctagaacg     3180

ttggagtagt taacaaacgt gatagatgta acctctggat catggtatga tgtcatttcc     3240

tagactagaa gaattggtag tcaaatcgag caaagtcccg aaagcacact gggctttcga     3300

cacagtgata ccaaagatgc tgaaaagaac tgaggcacga taaactgttc ggtgttggtg     3360

taaacgacca aagatgctga aaataactga tcgtcaccat ccgtgaatct aactttcgac     3420

acactgttac caaatccttc gtcaaaatta caggaataat taaggcgctt agacgatgat     3480

aaaccatttt ttgtcactaa ttaaccacac tgttctttgc ttgaccgtga caaaaaaaaa     3540

cttttttgtg aagcagtgtt gccgtaaacc acaaccatca tgaactcact tgccttgtca     3600

tatgtacttg taccatcgaa cgccgcgcgc taagacaatg caccaccctt caagtcttag     3660

ctcactgata ccgctaatta agttagataa tgtcgattac tagttgtctt acttcgaact     3720

atttcttttc ggcaaactga agtaaagaca acgttttgtt ccgcaggaca tgttcacggc     3780

gggtacggac acgtcgtcga tcatcgtgga gtgggcgatg gcggagatgc tcaagaaccc     3840

gaccgtcatg gcgcgcgcgc aggaggagct ggaccgcgcg gtgggccggg gccggcgcct     3900

ggaggagtcg gacctgcccg gcctccccta cctgcaggcg gtgtgcaagg aggccatgcg     3960

gctgcacccg tccacgccgc tcagcctccc gcacttctcc ttggacgcct gcgacgacgt     4020

cgacggctac cgcgtcccgg ccaacacccg cctgctcgtc aacgtctggg ccatcggccg     4080

ggacccggag gcctgggaga ggcccctcga cttccgcccc gagcgcttcc tgcccggggg     4140

cggcgcggag aaggtcgacc ccctggggaa ctgcttcgag ctcatcccgt tcggcgccgg     4200

ccggaggatc tgcgcgggga agctggcggg catggtgttc gtgcagtact tcctgggcac     4260

gctgctgcac gcgttcgact ggcgcctgcc tgacggcgag gagaagctgg acatgagcga     4320

gacgttcggc ctcgcgctgc ccaaggcagt gccgctccgc gccgtcgcca cgccacggct     4380

cgtgccggaa gcctatgcct ga                                              4402


<210>  12
<211>  387
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (101)..(287)
<223>  insertion of 187 nucleotides as present in QTL line

<400>  12
accatgcagc tcgcggcgtt gtgcaccgac cccgtggtgc tgtgcagcgc cttcctctgc       60

ctcctcctcc acgtggctct ccgctcgctg ctgcaccctc cttctgccca gaagcgggcc      120

cagacatttg agattgggta ttcaaaaatt caaaagatta aagaatttag tgttctaacg      180

ctattttatg caatacatta ttgacaaatt agtgttctaa cactatagat caccaaaaac      240

atgggtattc aatgaatacc catgaaaccc ccctgggccc gcccatgctt ctgccgcctc      300

ttcctccggg cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc      360

gctgccactc gtgggcccag ccccgca                                          387


<210>  13
<211>  201
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (101)..(101)
<223>  single nucleotide polymorphism

<400>  13
tgatgaaagc cgtgcaggag taaatagraa amtggagttg tggcgtgara ctctagaatc       60

caaacgtttt agactcttta gaactaaaac cgaatatata wgttgtgact ttggcactac      120

tacatatgac gaaggagata ttagtttgga aggtcaagta gtgccctgra aggatacctt      180

ttggtattta gtatcaatgc t                                                201


<210>  14
<211>  121
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (58)..(58)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  single nucleotide polymorphism

<400>  14
caaggaacct aggatggggg cggattcatt aacaaggagg ggggccctaa tcccctgncg       60

rtctgcacgg gcaataatct agtcagaccc cgaggtcagt agaggcggag gacgcgtcac      120

g                                                                      121


<210>  15
<211>  121
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (61)..(61)
<223>  single nucleotide polymorphism

<400>  15
cagcacatat tgtgttgaaa tgaatgagca cactgcatcc atatcaccac tggtacacaa       60

rggcatagtt taagtcatgt tatgatgtag cgcaccgagc aaggtatttt gacacacata      120

a                                                                      121


<210>  16
<211>  101
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (51)..(51)
<223>  single nucleotide polymorphism

<400>  16
aatgtgggaa gagctggagc agcaaatcta gacgtcattc aagaagtcga rtatgtaaag       60

gaagatgcta gaattatata cctccttgaa tgcctccaaa a                          101


<210>  17
<211>  101
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (51)..(51)
<223>  single nucleotide polymorphism

<400>  17
ctctttctgc agctcgtgat cgagttgtgt gtgctccatt cccaaattat rtatgatcga       60

ttagtagcgt taggggttaa ccagtagccg ctgtgtactt g                          101


<210>  18
<211>  201
<212>  DNA
<213>  Zea mays


<220>
<221>  misc_feature
<222>  (101)..(101)
<223>  single nucleotide polymorphism

<400>  18
ttatggggga ggcagattgt cgtggggcgc gggggagggc gagcttgggg gcgccgcaga       60

gcgagggaca ttgccgagat tgccatggag tgagggcacg rcgagattgc cgcgagggag      120

agggcgcttg ccgaggcaga ttgccgcggg ggaggggcgc ttgccgaggc ggagacgtgg      180

tgaagccgtg gtggacgccc a                                                201


<210>  19
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant P434L

<400>  19
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc ctcgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  20
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant P434L

<400>  20

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Leu Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  21
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant W426stop

<400>  21
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctagg agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  22
<211>  425
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant W426stop

<400>  22

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala 
            420                 425 


<210>  23
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant R252W

<400>  23
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgtggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  24
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant R252W

<400>  24

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Trp Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  25
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant R405H

<400>  25
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccacgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  26
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant R405H

<400>  26

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr His Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  27
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant P407L

<400>  27
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc tggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  28
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant P407L

<400>  28

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Leu Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  29
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant E427K

<400>  29
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctgga agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  30
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant E427K

<400>  30

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Lys Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  31
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant G450R

<400>  31
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctgag gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  32
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant G450R

<400>  32

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Arg Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  33
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant P429S

<400>  33
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggtccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  34
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant P429S

<400>  34

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Ser Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  35
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant P429L

<400>  35
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggctcct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  36
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant P429L

<400>  36

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Leu Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Arg Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  37
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant R436C

<400>  37
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggc agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc cccgagtgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  38
<211>  527
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant R436C

<400>  38

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly Gln Leu Pro Pro Gly 
        35                  40                  45              


Pro Pro Gly Leu Pro Ile Leu Gly Ala Leu Pro Leu Val Gly Pro Ala 
    50                  55                  60                  


Pro His Ala Gly Leu Ala Ala Leu Ala Arg Lys Tyr Gly Pro Ile Met 
65                  70                  75                  80  


Tyr Leu Lys Met Gly Thr Ala Gly Val Val Val Ala Ser Ser Pro Arg 
                85                  90                  95      


Ala Ala Arg Thr Phe Leu Lys Ala Leu Asp Ala Arg Tyr Ala Asn Arg 
            100                 105                 110         


Pro Ala Val Ala Ser Ala Ala Asp Ile Thr Tyr Gly Arg Gln Asn Met 
        115                 120                 125             


Val Phe Ala Asp Tyr Gly Pro Lys Trp Lys Leu Met Arg Lys Leu Ala 
    130                 135                 140                 


Ser Val His Leu Leu Gly Ala Arg Ala Leu Ala Asp Trp Ala Cys Val 
145                 150                 155                 160 


Arg Arg Gly Glu Ala Gly His Val Leu Arg Gly Val Ala Glu Ala Ala 
                165                 170                 175     


Ala Ala Gly Arg Pro Val Val Val Pro Glu Leu Leu Val Cys Ala Leu 
            180                 185                 190         


Ala Asn Ile Val Gly Gln Ile Thr Val Ser Lys Arg Val Phe Asp Ala 
        195                 200                 205             


Gln Gly Asp Asp Ser Asn Arg Tyr Lys Asp Met Ile Val Ser Leu Leu 
    210                 215                 220                 


Thr Gly Thr Gly Met Phe Asn Ile Ser Asp Phe Val Pro Ala Leu Ala 
225                 230                 235                 240 


Arg Leu Asp Leu Gln Gly Val Gln Ala Lys Leu Arg Arg Val His Arg 
                245                 250                 255     


Gln Phe Asp Gly Leu Ile Thr Lys Leu Leu Ala Glu His Ala Ala Thr 
            260                 265                 270         


Ala Ala Asp Arg Ala Arg Gln Gly Arg Pro Asp Phe Val Asp Arg Leu 
        275                 280                 285             


Arg Ala Thr Met Asp Ala Gly Ala Ala Ala Asp Asp Glu Ser Gly Glu 
    290                 295                 300                 


Thr Ile Thr Glu Val Asn Ile Lys Gly Leu Ile Phe Asp Met Phe Thr 
305                 310                 315                 320 


Ala Gly Thr Asp Thr Ser Ser Ile Ile Val Glu Trp Ala Met Ala Glu 
                325                 330                 335     


Met Leu Lys Asn Pro Thr Val Met Ala Arg Ala Gln Glu Glu Leu Asp 
            340                 345                 350         


Arg Ala Val Gly Arg Gly Arg Arg Leu Glu Glu Ser Asp Leu Pro Gly 
        355                 360                 365             


Leu Pro Tyr Leu Gln Ala Val Cys Lys Glu Ala Met Arg Leu His Pro 
    370                 375                 380                 


Ser Thr Pro Leu Ser Leu Pro His Phe Ser Leu Asp Ala Cys Asp Asp 
385                 390                 395                 400 


Val Asp Gly Tyr Arg Val Pro Ala Asn Thr Arg Leu Leu Val Asn Val 
                405                 410                 415     


Trp Ala Ile Gly Arg Asp Pro Glu Ala Trp Glu Arg Pro Leu Asp Phe 
            420                 425                 430         


Arg Pro Glu Cys Phe Leu Pro Gly Gly Gly Ala Glu Lys Val Asp Pro 
        435                 440                 445             


Leu Gly Asn Cys Phe Glu Leu Ile Pro Phe Gly Ala Gly Arg Arg Ile 
    450                 455                 460                 


Cys Ala Gly Lys Leu Ala Gly Met Val Phe Val Gln Tyr Phe Leu Gly 
465                 470                 475                 480 


Thr Leu Leu His Ala Phe Asp Trp Arg Leu Pro Asp Gly Glu Glu Lys 
                485                 490                 495     


Leu Asp Met Ser Glu Thr Phe Gly Leu Ala Leu Pro Lys Ala Val Pro 
            500                 505                 510         


Leu Arg Ala Val Ala Thr Pro Arg Leu Val Pro Glu Ala Tyr Ala 
        515                 520                 525         


<210>  39
<211>  4215
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  genomic DNA of F35H mutant Q44stop

<400>  39
atgcagctcg cggcgttgtg caccgacccc gtggtgctgt gcagcgcctt cctctgcctc       60

ctcctccacg tggctctccg ctcgctgctg caccctcctt ctgccgcctc ttcctccggg      120

cgccgcgggt agctcccgcc ggggccaccg ggcctgccga tcctcggcgc gctgccactc      180

gtgggcccag ccccgcacgc cggcctggcc gcgctggcgc gcaagtacgg tcccatcatg      240

tacctgaaga tgggcacggc cggcgtggtg gtggcgtcgt ccccgcgcgc ggcgcggacg      300

ttcctcaagg cgctggacgc gcggtacgcc aaccggccgg ccgtggcgag cgccgcggac      360

atcacgtacg ggcggcagaa catggtgttc gcggactacg ggcccaagtg gaagctgatg      420

cggaagctcg ccagcgtgca cctgctcggc gcgcgcgcgc tcgcggactg ggcgtgcgtg      480

cggcgcggcg aggccggcca cgtgctgcgc ggcgtggcgg aggcggccgc ggccggcagg      540

cccgtcgtcg tgccggagct gctcgtgtgc gccctcgcca acatcgtcgg gcagatcaca      600

gtgagcaagc gggtgttcga cgcgcagggg gacgactcga acaggtgagg atgggaggtc      660

catgaaatcc taccagctgt gagcatgcat aaaagttcat ttggaaagaa aagaacatat      720

ttttcttaca aatttatgct tactgtttct ttaagtttcg ataaagtttg taaaaaaaat      780

ttaggctagt ttgaaactcc atttaggatt tctattttcc aaagaaaaat aaacgaattt      840

ctcttgaaaa aatgaaaatt ctttagaaaa ataggttctc aaactagccc tcaataaaac      900

ttaatgcgat cgttttctct gactctcatt catctttctc tggttatcta attgggtcct      960

tgagagatga gtttacctgc ttgtccttta ttattgcaaa gacaacatat ctgatgcaca     1020

tggaacattg gtgcacatgg tgcacatatg aaatcatcac cactcatttt aaatctaacg     1080

tctatagttg tttgatatat tttattaagg acaccctcca acgtggtggt gtgtagtggt     1140

ggaaggtgtt atttgtaaat tgaataatca actagagacg ttagatctaa aatgagtggt     1200

gatgatttaa tatgtgcacc atgtgcacca gtcttctatg tgcaccagat atgtcctcta     1260

ttgcaaatgc tagacggaac accagctagc actagcagac tgtttatgtg gaaagaaaaa     1320

acttaaaaag atcagctagg aagctgctgt catctgtacg tatatatggt gaagactgaa     1380

caatctgcat gacaagcaaa acttagctta aaagcgaaaa gagcgatgga aacggccgct     1440

cgataaataa ttaatgagag tcttgggatt tttcatgcat ggaaaaaaac aaagctggca     1500

tttttcatct aatataatat ataacgctga tatcatattg cgtgcagata caaggacatg     1560

atcgtgtcgc tgctgaccgg cacgggcatg ttcaacatca gcgacttcgt gccggcgctg     1620

gcgcgtctgg acctgcaggg cgtgcaggcg aagctgcggc gcgtccaccg ccagttcgac     1680

ggcctcatca ccaagctgct ggccgagcac gccgcgacgg ccgcggaccg cgcgcgccag     1740

ggccgcccgg acttcgtcga ccggctccgc gccacgatgg acgccggcgc cgccgccgac     1800

gacgagagcg gcgagaccat caccgaggtc aacatcaagg gcctcatctt cgtaagctcc     1860

ctgctttttc ctcgccccca accatgcatc atcatatgca cttatatttt acacttgctc     1920

ggttttcctt tagtaactaa ctaatccgtc gcagctgcga tacacgtagc actagtacta     1980

cagcgatggg tcatcggtaa ctgaatctaa ggtgcaatag agtgcacggc cgcgggatca     2040

tggcgtgaca tgggagctaa gctaagccag tggccaccta acgaaggcac tgaccgaaag     2100

ctcagtggcg tgttaggtgg agatagtgga tcgagttgtt ggaaagacaa tatcaaaacc     2160

actctccaat tgatgatgtg tagggcctgc agtgttttga atccaccttg tttggtcgaa     2220

cacattacta gagtgaaata tggttccaat gttaattgat agcgcgaaag ggtctctagc     2280

gtaatggtta aaccttccga gtagcacatc caggttgggt tcgatcctct cgagggcgaa     2340

ttttcaagct ttgttaaaaa aattatctcg ttgtgccccg tccgctctca ggaatcgata     2400

ttctacacga caccctccga ctagtgacag ttgattgact cgttagtgat gagaagccat     2460

gctaaaaaag tggagacgta gatatgatag aggttccctt tcctaagcaa acgtgaatgc     2520

tatgaaaatt atgcagttta aaaaaaactt taaagataaa caggaattct cttttttgga     2580

acaaacaata cgaatgcacc tccaaatatc ttatcgagtc gacttttatg gaattattgt     2640

ttttgttatt tctaagatgg gagcccaaaa tcacatacaa attattcagt gaatgcctcg     2700

gtgttttttt attagttaag ggctctcatt tttttcaagg gatttttatt tttttccaaa     2760

agaaaataaa ctaatcctct ttagaaaaat ggaaatctat tggagaaatg aggttcctaa     2820

actagctcta acagtgagtc agttaatcag gagaagatat tagactcctg tatagtgtgc     2880

agcaaccaca tccgattctg acgttttagc ttaatgttcg ctatgtagac gtcgggcata     2940

gggaatgcat tgctaccaga acacgaatga cagctatgca agtctctaga acgttggagt     3000

agttaacaaa cgtgatagat gtaacctctg gatcatggta tgatgtcatt tcctagacta     3060

gaagaattgg tagtcaaatc gagcaaagtc ccgaaagcac actgggcttt cgacacagtg     3120

ataccaaaga tgctgaaaag aactgaggca cgataaactg ttcggtgttg gtgtaaacga     3180

ccaaagatgc tgaaaataac tgatcgtcac catccgtgaa tctaactttc gacacactgt     3240

taccaaatcc ttcgtcaaaa ttacaggaat aattaaggcg cttagacgat gataaaccat     3300

tttttgtcac taattaacca cactgttctt tgcttgaccg tgacaaaaaa aaactttttt     3360

gtgaagcagt gttgccgtaa accacaacca tcatgaactc acttgccttg tcatatgtac     3420

ttgtaccatc gaacgccgcg cgctaagaca atgcaccacc cttcaagtct tagctcactg     3480

ataccgctaa ttaagttaga taatgtcgat tactagttgt cttacttcga actatttctt     3540

ttcggcaaac tgaagtaaag acaacgtttt gttccgcagg acatgttcac ggcgggtacg     3600

gacacgtcgt cgatcatcgt ggagtgggcg atggcggaga tgctcaagaa cccgaccgtc     3660

atggcgcgcg cgcaggagga gctggaccgc gcggtgggcc ggggccggcg cctggaggag     3720

tcggacctgc ccggcctccc ctacctgcag gcggtgtgca aggaggccat gcggctgcac     3780

ccgtccacgc cgctcagcct cccgcacttc tccttggacg cctgcgacga cgtcgacggc     3840

taccgcgtcc cggccaacac ccgcctgctc gtcaacgtct gggccatcgg ccgggacccg     3900

gaggcctggg agaggcccct cgacttccgc cccgagcgct tcctgcccgg gggcggcgcg     3960

gagaaggtcg accccctggg gaactgcttc gagctcatcc cgttcggcgc cggccggagg     4020

atctgcgcgg ggaagctggc gggcatggtg ttcgtgcagt acttcctggg cacgctgctg     4080

cacgcgttcg actggcgcct gcctgacggc gaggagaagc tggacatgag cgagacgttc     4140

ggcctcgcgc tgcccaaggc agtgccgctc cgcgccgtcg ccacgccacg gctcgtgccg     4200

gaagcctatg cctga                                                      4215


<210>  40
<211>  43
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  amino acid sequence of F35H mutant Q44stop

<400>  40

Met Gln Leu Ala Ala Leu Cys Thr Asp Pro Val Val Leu Cys Ser Ala 
1               5                   10                  15      


Phe Leu Cys Leu Leu Leu His Val Ala Leu Arg Ser Leu Leu His Pro 
            20                  25                  30          


Pro Ser Ala Ala Ser Ser Ser Gly Arg Arg Gly 
        35                  40              


