                         SEQUENCE LISTING

<110>  LONDON SCHOOL OF HYGIENE & TROPICAL MEDICINE
       UCL BUSINESS PLC
 
<120>  GLYCONJUGATE VACCINES

<130>  007462179

<140>  PCT/EP2019/061266
<141>  2019-05-02

<150>  GB 1807303.1
<151>  2018-04-03

<160>  41    

<170>  PatentIn version 3.5

<210>  1
<211>  1138
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  (PiuA coding sequence) with sequons

<400>  1
ggatccgaat tccattttaa ataaggagga ataacataat gaaaaagatt tggctggcgc       60

tggctggttt agttttagcg tttagcgcat cggcggcgca gggaggcgat cagaacgcga      120

ccggtggcgc cctagtggcc agcttcttgc tcctacttgg tgcatgtagt acaaactcaa      180

gcactagtca gacagagacc agtagctctg ctccaacaga ggtaaccatt aaaagttcac      240

tggacgaggt caaactttcc aaagttcctg aaaagattgt gacctttgac ctcggcgctg      300

cggatactat tcgcgcttta ggatttgaaa aaaatatcgt cggaatgcct acaaaaactg      360

ttccgactta tctaaaagac ctagtgggaa ctgtcaaaaa tgttggttct atgaaagaac      420

ctgatttaga agctatcgcc gcccttgagc ctgatttgat tatcgcttcg ccacgtacac      480

aaaaattcgt agacaaattc aaagaaatcg ccccaaccgt tctcttccaa gcaagcaagg      540

acgactactg gacttctacc aaggctaata tcgaatcctt agcaagtgcc ttcggcgaaa      600

ctggtacaca gaaagccaag gaagaattga ccaagctaga caagagcatc caagaagtcg      660

ctactaaaaa tgaaagctct gacaaaaaag cccttgcgat cctccttaat gaaggaaaaa      720

tggcagcctt tggtgccaaa tctcgtttct ctttcttgta ccaaaccttg aaattcaaac      780

caactgatac aaaatttgaa gactcacgcc acggacaaga agtcagcttt gaaagtgtca      840

aagaaatcaa ccctgacatc ctctttgtca tcaaccgtac ccttgccatc ggtggggaca      900

actctagcaa cgacggtgtc ctagaaaatg cccttatcgc tgaaacacct gctgctaaaa      960

atggtaagat tatccaacta acaccagacc tctggtatct aagcggaggc ggacttgaat     1020

caacaaaact catgattgaa gacatacaaa aagctttgaa aggaggcgat cagaacgcga     1080

ccggtggcca tcaccatcat caccatcatc accatcatta attaagtcta gaggatcc       1138


<210>  2
<211>  360
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  2 PiuA amino acid sequence with sequons,His tag and leader 
       peptide

<400>  2

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Ala Gln Gly Gly Asp Gln Asn Ala Thr Gly Gly Ala Leu 
            20                  25                  30          


Val Ala Ser Phe Leu Leu Leu Leu Gly Ala Cys Ser Thr Asn Ser Ser 
        35                  40                  45              


Thr Ser Gln Thr Glu Thr Ser Ser Ser Ala Pro Thr Glu Val Thr Ile 
    50                  55                  60                  


Lys Ser Ser Leu Asp Glu Val Lys Leu Ser Lys Val Pro Glu Lys Ile 
65                  70                  75                  80  


Val Thr Phe Asp Leu Gly Ala Ala Asp Thr Ile Arg Ala Leu Gly Phe 
                85                  90                  95      


Glu Lys Asn Ile Val Gly Met Pro Thr Lys Thr Val Pro Thr Tyr Leu 
            100                 105                 110         


Lys Asp Leu Val Gly Thr Val Lys Asn Val Gly Ser Met Lys Glu Pro 
        115                 120                 125             


Asp Leu Glu Ala Ile Ala Ala Leu Glu Pro Asp Leu Ile Ile Ala Ser 
    130                 135                 140                 


Pro Arg Thr Gln Lys Phe Val Asp Lys Phe Lys Glu Ile Ala Pro Thr 
145                 150                 155                 160 


Val Leu Phe Gln Ala Ser Lys Asp Asp Tyr Trp Thr Ser Thr Lys Ala 
                165                 170                 175     


Asn Ile Glu Ser Leu Ala Ser Ala Phe Gly Glu Thr Gly Thr Gln Lys 
            180                 185                 190         


Ala Lys Glu Glu Leu Thr Lys Leu Asp Lys Ser Ile Gln Glu Val Ala 
        195                 200                 205             


Thr Lys Asn Glu Ser Ser Asp Lys Lys Ala Leu Ala Ile Leu Leu Asn 
    210                 215                 220                 


Glu Gly Lys Met Ala Ala Phe Gly Ala Lys Ser Arg Phe Ser Phe Leu 
225                 230                 235                 240 


Tyr Gln Thr Leu Lys Phe Lys Pro Thr Asp Thr Lys Phe Glu Asp Ser 
                245                 250                 255     


Arg His Gly Gln Glu Val Ser Phe Glu Ser Val Lys Glu Ile Asn Pro 
            260                 265                 270         


Asp Ile Leu Phe Val Ile Asn Arg Thr Leu Ala Ile Gly Gly Asp Asn 
        275                 280                 285             


Ser Ser Asn Asp Gly Val Leu Glu Asn Ala Leu Ile Ala Glu Thr Pro 
    290                 295                 300                 


Ala Ala Lys Asn Gly Lys Ile Ile Gln Leu Thr Pro Asp Leu Trp Tyr 
305                 310                 315                 320 


Leu Ser Gly Gly Gly Leu Glu Ser Thr Lys Leu Met Ile Glu Asp Ile 
                325                 330                 335     


Gln Lys Ala Leu Lys Gly Gly Asp Gln Asn Ala Thr Gly Gly His His 
            340                 345                 350         


His His His His His His His His 
        355                 360 


<210>  3
<211>  311
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  (PiuA amino acid sequence)

<400>  3

Ala Leu Val Ala Ser Phe Leu Leu Leu Leu Gly Ala Cys Ser Thr Asn 
1               5                   10                  15      


Ser Ser Thr Ser Gln Thr Glu Thr Ser Ser Ser Ala Pro Thr Glu Val 
            20                  25                  30          


Thr Ile Lys Ser Ser Leu Asp Glu Val Lys Leu Ser Lys Val Pro Glu 
        35                  40                  45              


Lys Ile Val Thr Phe Asp Leu Gly Ala Ala Asp Thr Ile Arg Ala Leu 
    50                  55                  60                  


Gly Phe Glu Lys Asn Ile Val Gly Met Pro Thr Lys Thr Val Pro Thr 
65                  70                  75                  80  


Tyr Leu Lys Asp Leu Val Gly Thr Val Lys Asn Val Gly Ser Met Lys 
                85                  90                  95      


Glu Pro Asp Leu Glu Ala Ile Ala Ala Leu Glu Pro Asp Leu Ile Ile 
            100                 105                 110         


Ala Ser Pro Arg Thr Gln Lys Phe Val Asp Lys Phe Lys Glu Ile Ala 
        115                 120                 125             


Pro Thr Val Leu Phe Gln Ala Ser Lys Asp Asp Tyr Trp Thr Ser Thr 
    130                 135                 140                 


Lys Ala Asn Ile Glu Ser Leu Ala Ser Ala Phe Gly Glu Thr Gly Thr 
145                 150                 155                 160 


Gln Lys Ala Lys Glu Glu Leu Thr Lys Leu Asp Lys Ser Ile Gln Glu 
                165                 170                 175     


Val Ala Thr Lys Asn Glu Ser Ser Asp Lys Lys Ala Leu Ala Ile Leu 
            180                 185                 190         


Leu Asn Glu Gly Lys Met Ala Ala Phe Gly Ala Lys Ser Arg Phe Ser 
        195                 200                 205             


Phe Leu Tyr Gln Thr Leu Lys Phe Lys Pro Thr Asp Thr Lys Phe Glu 
    210                 215                 220                 


Asp Ser Arg His Gly Gln Glu Val Ser Phe Glu Ser Val Lys Glu Ile 
225                 230                 235                 240 


Asn Pro Asp Ile Leu Phe Val Ile Asn Arg Thr Leu Ala Ile Gly Gly 
                245                 250                 255     


Asp Asn Ser Ser Asn Asp Gly Val Leu Glu Asn Ala Leu Ile Ala Glu 
            260                 265                 270         


Thr Pro Ala Ala Lys Asn Gly Lys Ile Ile Gln Leu Thr Pro Asp Leu 
        275                 280                 285             


Trp Tyr Leu Ser Gly Gly Gly Leu Glu Ser Thr Lys Leu Met Ile Glu 
    290                 295                 300                 


Asp Ile Gln Lys Ala Leu Lys 
305                 310     


<210>  4
<211>  3136
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  NanA coding sequence

<400>  4
ggatccgaat tccattttaa ataaggagga ataacataat gaaaaagatt tggctggcgc       60

tggctggttt agttttagcg tttagcgcat cggcggcgca gggaggcgat cagaacgcga      120

ccggtggcga gcaacctctg gcaaatgaaa ctcaactttc gggggagagc tcaaccctaa      180

ctgatacaga aaagagccag ccttcttcag agactgaact ttctggcaat aagcaagaac      240

aagaaaggaa agataagcaa gaagaaaaaa ttccaagaga ttactatgca cgagatttgg      300

aaaatgtcga aacagtgata gaaaaagaag atgttgaaac caatgcttca aatggtcaga      360

gagttgattt atcaagtgaa ctagataaac taaagaaact tgaaaacgca acagttcaca      420

tggagtttaa gccagatgcc aaggccccag cattctataa tctcttttct gtgtcaagtg      480

ctactaaaaa agatgagtac ttcactatgg cagtttacaa taatactgct accctagagg      540

ggcgtggttc ggatgggaaa cagttttaca ataattacaa cgatgcaccc ttaaaagtta      600

aaccaggtca gtggaactct gtgactttca cagttgaaaa accgacagca gaactaccta      660

aaggccgagt gcgcctctac gtaaacgggg tattatctcg aacaagtctg agatctggca      720

atttcattaa agatatgcca gatgtaacgc atgtgcaaat cggagcaacc aagcgtgcca      780

acaatacggt ttgggggtca aatctacaga ttcggaatct cactgtgtat aatcgtgctt      840

taacaccaga agaggtacaa aaacgtagtc aactttttaa acgctcagat ttagaaaaaa      900

aactacctga aggagcggct ttaacagaga aaacggacat attcgaaagc gggcgtaacg      960

gtaaaccaaa taaagatgga atcaagagtt atcgtattcc agcacttctc aagacagata     1020

aaggaacttt gatcgcaggt gcagatgaac gccgtctcca ttcgagtgac tggggtgata     1080

tcggtatggt catcagacgt agtgaagata atggtaaaac ttggggtgac cgagtaacca     1140

ttaccaactt acgtgacaat ccaaaagctt ctgacccatc gatcggttca ccagtgaata     1200

tcgatatggt gttggttcaa gatcctgaaa ccaaacgaat cttttctatc tatgacatgt     1260

tcccagaagg gaagggaatc tttggaatgt cttcacaaaa agaagaagcc tacaaaaaaa     1320

tcgatggaaa aacctatcaa atcctctatc gtgaaggaga aaagggagct tataccattc     1380

gagaaaatgg tactgtctat acaccagatg gtaaggcgac agactatcgc gttgttgtag     1440

atcctgttaa accagcctat agcgacaagg gggatctata caagggtaac caattactag     1500

gcaatatcta cttcacaaca aacaaaactt ctccatttag aattgccaag gatagctatc     1560

tatggatgtc ctacagtgat gacgacggga agacatggtc agcgcctcaa gatattactc     1620

cgatggtcaa agccgattgg atgaaattct tgggtgtagg tcctggaaca ggaattgtac     1680

ttcggaatgg gcctcacaag ggacggattt tgataccggt ttatacgact aataatgtat     1740

ctcacttaaa tggctcgcaa tcttctcgta tcatctattc agatgatcat ggaaaaactt     1800

ggcatgctgg agaagcggtc aacgataacc gtcaggtaga cggtcaaaag atccactctt     1860

ctacgatgaa caatagacgt gcgcaaaata cagaatcaac ggtggtacaa ctaaacaatg     1920

gagatgttaa actctttatg cgtggtttga ctggagatct tcaggttgct acaagtaaag     1980

acggaggagt gacttgggag aaggatatca aacgttatcc acaggttaaa gatgtctatg     2040

ttcaaatgtc tgctatccat acgatgcacg aaggaaaaga atacatcatc ctcagtaatg     2100

caggtggacc gaaacgtgaa aatgggatgg tccacttggc acgtgtcgaa gaaaatggtg     2160

agttgacttg gctcaaacac aatccaattc aaaaaggaga gtttgcctat aattcgctcc     2220

aagaattagg aaatggggag tatggcatct tgtatgaaca tactgaaaaa ggacaaaatg     2280

cctataccct atcatttaga aaatttaatt gggacttttt gagcaaagat ctgatttctc     2340

ctaccgaagc gaaagtgaag cgaactagag agatgggcaa aggagttatt ggcttggagt     2400

tcgactcaga agtattggtc aacaaggctc caacccttca attggcaaat ggtaaaacag     2460

cacgcttcat gacccagtat gatacaaaaa ccctcctatt tacagtggat tcagaggata     2520

tgggtcaaaa agttacaggt ttggcagaag gtgcaattga aagtatgcat aatttaccag     2580

tctctgtggc gggcactaag ctttcgaatg gaatgaacgg aagtgaagct gctgttcatg     2640

aagtgccaga atacacaggc ccattaggga catccggcga agagccagct ccaacagtcg     2700

agaagccaga atacacaggc ccactaggga catccggcga agagccagcc ccgacagtcg     2760

agaagccaga atacacaggc ccactaggga cagctggtga agaagcagct ccaacagtcg     2820

agaagccaga atttacaggg ggagttaatg gtacagagcc agctgttcat gaaatcgcag     2880

agtataaggg atctgattcg cttgtaactc ttactacaaa agaagattat acttacaaag     2940

ctcctcttgc tcagcaggca cttcctgaaa caggaaacaa ggagagtgac ctcctagctt     3000

cactaggact aacagctttc ttccttggtc tgtttacgct agggaaaaag agagaacaag     3060

gaggcgatca gaacgcgacc ggtggccatc accatcatca ccatcatcac catcattaat     3120

taagtctaga ggatcc                                                     3136


<210>  5
<211>  1026
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NanA amino acid sequence with sequons,His tag and leader peptide

<400>  5

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Ala Gln Gly Gly Asp Gln Asn Ala Thr Gly Gly Glu Gln 
            20                  25                  30          


Pro Leu Ala Asn Glu Thr Gln Leu Ser Gly Glu Ser Ser Thr Leu Thr 
        35                  40                  45              


Asp Thr Glu Lys Ser Gln Pro Ser Ser Glu Thr Glu Leu Ser Gly Asn 
    50                  55                  60                  


Lys Gln Glu Gln Glu Arg Lys Asp Lys Gln Glu Glu Lys Ile Pro Arg 
65                  70                  75                  80  


Asp Tyr Tyr Ala Arg Asp Leu Glu Asn Val Glu Thr Val Ile Glu Lys 
                85                  90                  95      


Glu Asp Val Glu Thr Asn Ala Ser Asn Gly Gln Arg Val Asp Leu Ser 
            100                 105                 110         


Ser Glu Leu Asp Lys Leu Lys Lys Leu Glu Asn Ala Thr Val His Met 
        115                 120                 125             


Glu Phe Lys Pro Asp Ala Lys Ala Pro Ala Phe Tyr Asn Leu Phe Ser 
    130                 135                 140                 


Val Ser Ser Ala Thr Lys Lys Asp Glu Tyr Phe Thr Met Ala Val Tyr 
145                 150                 155                 160 


Asn Asn Thr Ala Thr Leu Glu Gly Arg Gly Ser Asp Gly Lys Gln Phe 
                165                 170                 175     


Tyr Asn Asn Tyr Asn Asp Ala Pro Leu Lys Val Lys Pro Gly Gln Trp 
            180                 185                 190         


Asn Ser Val Thr Phe Thr Val Glu Lys Pro Thr Ala Glu Leu Pro Lys 
        195                 200                 205             


Gly Arg Val Arg Leu Tyr Val Asn Gly Val Leu Ser Arg Thr Ser Leu 
    210                 215                 220                 


Arg Ser Gly Asn Phe Ile Lys Asp Met Pro Asp Val Thr His Val Gln 
225                 230                 235                 240 


Ile Gly Ala Thr Lys Arg Ala Asn Asn Thr Val Trp Gly Ser Asn Leu 
                245                 250                 255     


Gln Ile Arg Asn Leu Thr Val Tyr Asn Arg Ala Leu Thr Pro Glu Glu 
            260                 265                 270         


Val Gln Lys Arg Ser Gln Leu Phe Lys Arg Ser Asp Leu Glu Lys Lys 
        275                 280                 285             


Leu Pro Glu Gly Ala Ala Leu Thr Glu Lys Thr Asp Ile Phe Glu Ser 
    290                 295                 300                 


Gly Arg Asn Gly Lys Pro Asn Lys Asp Gly Ile Lys Ser Tyr Arg Ile 
305                 310                 315                 320 


Pro Ala Leu Leu Lys Thr Asp Lys Gly Thr Leu Ile Ala Gly Ala Asp 
                325                 330                 335     


Glu Arg Arg Leu His Ser Ser Asp Trp Gly Asp Ile Gly Met Val Ile 
            340                 345                 350         


Arg Arg Ser Glu Asp Asn Gly Lys Thr Trp Gly Asp Arg Val Thr Ile 
        355                 360                 365             


Thr Asn Leu Arg Asp Asn Pro Lys Ala Ser Asp Pro Ser Ile Gly Ser 
    370                 375                 380                 


Pro Val Asn Ile Asp Met Val Leu Val Gln Asp Pro Glu Thr Lys Arg 
385                 390                 395                 400 


Ile Phe Ser Ile Tyr Asp Met Phe Pro Glu Gly Lys Gly Ile Phe Gly 
                405                 410                 415     


Met Ser Ser Gln Lys Glu Glu Ala Tyr Lys Lys Ile Asp Gly Lys Thr 
            420                 425                 430         


Tyr Gln Ile Leu Tyr Arg Glu Gly Glu Lys Gly Ala Tyr Thr Ile Arg 
        435                 440                 445             


Glu Asn Gly Thr Val Tyr Thr Pro Asp Gly Lys Ala Thr Asp Tyr Arg 
    450                 455                 460                 


Val Val Val Asp Pro Val Lys Pro Ala Tyr Ser Asp Lys Gly Asp Leu 
465                 470                 475                 480 


Tyr Lys Gly Asn Gln Leu Leu Gly Asn Ile Tyr Phe Thr Thr Asn Lys 
                485                 490                 495     


Thr Ser Pro Phe Arg Ile Ala Lys Asp Ser Tyr Leu Trp Met Ser Tyr 
            500                 505                 510         


Ser Asp Asp Asp Gly Lys Thr Trp Ser Ala Pro Gln Asp Ile Thr Pro 
        515                 520                 525             


Met Val Lys Ala Asp Trp Met Lys Phe Leu Gly Val Gly Pro Gly Thr 
    530                 535                 540                 


Gly Ile Val Leu Arg Asn Gly Pro His Lys Gly Arg Ile Leu Ile Pro 
545                 550                 555                 560 


Val Tyr Thr Thr Asn Asn Val Ser His Leu Asn Gly Ser Gln Ser Ser 
                565                 570                 575     


Arg Ile Ile Tyr Ser Asp Asp His Gly Lys Thr Trp His Ala Gly Glu 
            580                 585                 590         


Ala Val Asn Asp Asn Arg Gln Val Asp Gly Gln Lys Ile His Ser Ser 
        595                 600                 605             


Thr Met Asn Asn Arg Arg Ala Gln Asn Thr Glu Ser Thr Val Val Gln 
    610                 615                 620                 


Leu Asn Asn Gly Asp Val Lys Leu Phe Met Arg Gly Leu Thr Gly Asp 
625                 630                 635                 640 


Leu Gln Val Ala Thr Ser Lys Asp Gly Gly Val Thr Trp Glu Lys Asp 
                645                 650                 655     


Ile Lys Arg Tyr Pro Gln Val Lys Asp Val Tyr Val Gln Met Ser Ala 
            660                 665                 670         


Ile His Thr Met His Glu Gly Lys Glu Tyr Ile Ile Leu Ser Asn Ala 
        675                 680                 685             


Gly Gly Pro Lys Arg Glu Asn Gly Met Val His Leu Ala Arg Val Glu 
    690                 695                 700                 


Glu Asn Gly Glu Leu Thr Trp Leu Lys His Asn Pro Ile Gln Lys Gly 
705                 710                 715                 720 


Glu Phe Ala Tyr Asn Ser Leu Gln Glu Leu Gly Asn Gly Glu Tyr Gly 
                725                 730                 735     


Ile Leu Tyr Glu His Thr Glu Lys Gly Gln Asn Ala Tyr Thr Leu Ser 
            740                 745                 750         


Phe Arg Lys Phe Asn Trp Asp Phe Leu Ser Lys Asp Leu Ile Ser Pro 
        755                 760                 765             


Thr Glu Ala Lys Val Lys Arg Thr Arg Glu Met Gly Lys Gly Val Ile 
    770                 775                 780                 


Gly Leu Glu Phe Asp Ser Glu Val Leu Val Asn Lys Ala Pro Thr Leu 
785                 790                 795                 800 


Gln Leu Ala Asn Gly Lys Thr Ala Arg Phe Met Thr Gln Tyr Asp Thr 
                805                 810                 815     


Lys Thr Leu Leu Phe Thr Val Asp Ser Glu Asp Met Gly Gln Lys Val 
            820                 825                 830         


Thr Gly Leu Ala Glu Gly Ala Ile Glu Ser Met His Asn Leu Pro Val 
        835                 840                 845             


Ser Val Ala Gly Thr Lys Leu Ser Asn Gly Met Asn Gly Ser Glu Ala 
    850                 855                 860                 


Ala Val His Glu Val Pro Glu Tyr Thr Gly Pro Leu Gly Thr Ser Gly 
865                 870                 875                 880 


Glu Glu Pro Ala Pro Thr Val Glu Lys Pro Glu Tyr Thr Gly Pro Leu 
                885                 890                 895     


Gly Thr Ser Gly Glu Glu Pro Ala Pro Thr Val Glu Lys Pro Glu Tyr 
            900                 905                 910         


Thr Gly Pro Leu Gly Thr Ala Gly Glu Glu Ala Ala Pro Thr Val Glu 
        915                 920                 925             


Lys Pro Glu Phe Thr Gly Gly Val Asn Gly Thr Glu Pro Ala Val His 
    930                 935                 940                 


Glu Ile Ala Glu Tyr Lys Gly Ser Asp Ser Leu Val Thr Leu Thr Thr 
945                 950                 955                 960 


Lys Glu Asp Tyr Thr Tyr Lys Ala Pro Leu Ala Gln Gln Ala Leu Pro 
                965                 970                 975     


Glu Thr Gly Asn Lys Glu Ser Asp Leu Leu Ala Ser Leu Gly Leu Thr 
            980                 985                 990         


Ala Phe Phe Leu Gly Leu Phe Thr  Leu Gly Lys Lys Arg  Glu Gln Gly 
        995                 1000                 1005             


Gly Asp  Gln Asn Ala Thr Gly  Gly His His His His  His His His 
    1010                 1015                 1020             


His His  His 
    1025     


<210>  6
<211>  977
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  NanA amino acid sequence

<400>  6

Glu Gln Pro Leu Ala Asn Glu Thr Gln Leu Ser Gly Glu Ser Ser Thr 
1               5                   10                  15      


Leu Thr Asp Thr Glu Lys Ser Gln Pro Ser Ser Glu Thr Glu Leu Ser 
            20                  25                  30          


Gly Asn Lys Gln Glu Gln Glu Arg Lys Asp Lys Gln Glu Glu Lys Ile 
        35                  40                  45              


Pro Arg Asp Tyr Tyr Ala Arg Asp Leu Glu Asn Val Glu Thr Val Ile 
    50                  55                  60                  


Glu Lys Glu Asp Val Glu Thr Asn Ala Ser Asn Gly Gln Arg Val Asp 
65                  70                  75                  80  


Leu Ser Ser Glu Leu Asp Lys Leu Lys Lys Leu Glu Asn Ala Thr Val 
                85                  90                  95      


His Met Glu Phe Lys Pro Asp Ala Lys Ala Pro Ala Phe Tyr Asn Leu 
            100                 105                 110         


Phe Ser Val Ser Ser Ala Thr Lys Lys Asp Glu Tyr Phe Thr Met Ala 
        115                 120                 125             


Val Tyr Asn Asn Thr Ala Thr Leu Glu Gly Arg Gly Ser Asp Gly Lys 
    130                 135                 140                 


Gln Phe Tyr Asn Asn Tyr Asn Asp Ala Pro Leu Lys Val Lys Pro Gly 
145                 150                 155                 160 


Gln Trp Asn Ser Val Thr Phe Thr Val Glu Lys Pro Thr Ala Glu Leu 
                165                 170                 175     


Pro Lys Gly Arg Val Arg Leu Tyr Val Asn Gly Val Leu Ser Arg Thr 
            180                 185                 190         


Ser Leu Arg Ser Gly Asn Phe Ile Lys Asp Met Pro Asp Val Thr His 
        195                 200                 205             


Val Gln Ile Gly Ala Thr Lys Arg Ala Asn Asn Thr Val Trp Gly Ser 
    210                 215                 220                 


Asn Leu Gln Ile Arg Asn Leu Thr Val Tyr Asn Arg Ala Leu Thr Pro 
225                 230                 235                 240 


Glu Glu Val Gln Lys Arg Ser Gln Leu Phe Lys Arg Ser Asp Leu Glu 
                245                 250                 255     


Lys Lys Leu Pro Glu Gly Ala Ala Leu Thr Glu Lys Thr Asp Ile Phe 
            260                 265                 270         


Glu Ser Gly Arg Asn Gly Lys Pro Asn Lys Asp Gly Ile Lys Ser Tyr 
        275                 280                 285             


Arg Ile Pro Ala Leu Leu Lys Thr Asp Lys Gly Thr Leu Ile Ala Gly 
    290                 295                 300                 


Ala Asp Glu Arg Arg Leu His Ser Ser Asp Trp Gly Asp Ile Gly Met 
305                 310                 315                 320 


Val Ile Arg Arg Ser Glu Asp Asn Gly Lys Thr Trp Gly Asp Arg Val 
                325                 330                 335     


Thr Ile Thr Asn Leu Arg Asp Asn Pro Lys Ala Ser Asp Pro Ser Ile 
            340                 345                 350         


Gly Ser Pro Val Asn Ile Asp Met Val Leu Val Gln Asp Pro Glu Thr 
        355                 360                 365             


Lys Arg Ile Phe Ser Ile Tyr Asp Met Phe Pro Glu Gly Lys Gly Ile 
    370                 375                 380                 


Phe Gly Met Ser Ser Gln Lys Glu Glu Ala Tyr Lys Lys Ile Asp Gly 
385                 390                 395                 400 


Lys Thr Tyr Gln Ile Leu Tyr Arg Glu Gly Glu Lys Gly Ala Tyr Thr 
                405                 410                 415     


Ile Arg Glu Asn Gly Thr Val Tyr Thr Pro Asp Gly Lys Ala Thr Asp 
            420                 425                 430         


Tyr Arg Val Val Val Asp Pro Val Lys Pro Ala Tyr Ser Asp Lys Gly 
        435                 440                 445             


Asp Leu Tyr Lys Gly Asn Gln Leu Leu Gly Asn Ile Tyr Phe Thr Thr 
    450                 455                 460                 


Asn Lys Thr Ser Pro Phe Arg Ile Ala Lys Asp Ser Tyr Leu Trp Met 
465                 470                 475                 480 


Ser Tyr Ser Asp Asp Asp Gly Lys Thr Trp Ser Ala Pro Gln Asp Ile 
                485                 490                 495     


Thr Pro Met Val Lys Ala Asp Trp Met Lys Phe Leu Gly Val Gly Pro 
            500                 505                 510         


Gly Thr Gly Ile Val Leu Arg Asn Gly Pro His Lys Gly Arg Ile Leu 
        515                 520                 525             


Ile Pro Val Tyr Thr Thr Asn Asn Val Ser His Leu Asn Gly Ser Gln 
    530                 535                 540                 


Ser Ser Arg Ile Ile Tyr Ser Asp Asp His Gly Lys Thr Trp His Ala 
545                 550                 555                 560 


Gly Glu Ala Val Asn Asp Asn Arg Gln Val Asp Gly Gln Lys Ile His 
                565                 570                 575     


Ser Ser Thr Met Asn Asn Arg Arg Ala Gln Asn Thr Glu Ser Thr Val 
            580                 585                 590         


Val Gln Leu Asn Asn Gly Asp Val Lys Leu Phe Met Arg Gly Leu Thr 
        595                 600                 605             


Gly Asp Leu Gln Val Ala Thr Ser Lys Asp Gly Gly Val Thr Trp Glu 
    610                 615                 620                 


Lys Asp Ile Lys Arg Tyr Pro Gln Val Lys Asp Val Tyr Val Gln Met 
625                 630                 635                 640 


Ser Ala Ile His Thr Met His Glu Gly Lys Glu Tyr Ile Ile Leu Ser 
                645                 650                 655     


Asn Ala Gly Gly Pro Lys Arg Glu Asn Gly Met Val His Leu Ala Arg 
            660                 665                 670         


Val Glu Glu Asn Gly Glu Leu Thr Trp Leu Lys His Asn Pro Ile Gln 
        675                 680                 685             


Lys Gly Glu Phe Ala Tyr Asn Ser Leu Gln Glu Leu Gly Asn Gly Glu 
    690                 695                 700                 


Tyr Gly Ile Leu Tyr Glu His Thr Glu Lys Gly Gln Asn Ala Tyr Thr 
705                 710                 715                 720 


Leu Ser Phe Arg Lys Phe Asn Trp Asp Phe Leu Ser Lys Asp Leu Ile 
                725                 730                 735     


Ser Pro Thr Glu Ala Lys Val Lys Arg Thr Arg Glu Met Gly Lys Gly 
            740                 745                 750         


Val Ile Gly Leu Glu Phe Asp Ser Glu Val Leu Val Asn Lys Ala Pro 
        755                 760                 765             


Thr Leu Gln Leu Ala Asn Gly Lys Thr Ala Arg Phe Met Thr Gln Tyr 
    770                 775                 780                 


Asp Thr Lys Thr Leu Leu Phe Thr Val Asp Ser Glu Asp Met Gly Gln 
785                 790                 795                 800 


Lys Val Thr Gly Leu Ala Glu Gly Ala Ile Glu Ser Met His Asn Leu 
                805                 810                 815     


Pro Val Ser Val Ala Gly Thr Lys Leu Ser Asn Gly Met Asn Gly Ser 
            820                 825                 830         


Glu Ala Ala Val His Glu Val Pro Glu Tyr Thr Gly Pro Leu Gly Thr 
        835                 840                 845             


Ser Gly Glu Glu Pro Ala Pro Thr Val Glu Lys Pro Glu Tyr Thr Gly 
    850                 855                 860                 


Pro Leu Gly Thr Ser Gly Glu Glu Pro Ala Pro Thr Val Glu Lys Pro 
865                 870                 875                 880 


Glu Tyr Thr Gly Pro Leu Gly Thr Ala Gly Glu Glu Ala Ala Pro Thr 
                885                 890                 895     


Val Glu Lys Pro Glu Phe Thr Gly Gly Val Asn Gly Thr Glu Pro Ala 
            900                 905                 910         


Val His Glu Ile Ala Glu Tyr Lys Gly Ser Asp Ser Leu Val Thr Leu 
        915                 920                 925             


Thr Thr Lys Glu Asp Tyr Thr Tyr Lys Ala Pro Leu Ala Gln Gln Ala 
    930                 935                 940                 


Leu Pro Glu Thr Gly Asn Lys Glu Ser Asp Leu Leu Ala Ser Leu Gly 
945                 950                 955                 960 


Leu Thr Ala Phe Phe Leu Gly Leu Phe Thr Leu Gly Lys Lys Arg Glu 
                965                 970                 975     


Gln 
    


<210>  7
<211>  1003
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Sp0148 coding sequence

<400>  7
ggatccgaat tccattttaa ataaggagga ataacataat gaaaaagatt tggctggcgc       60

tggctggttt agttttagcg tttagcgcat cggcggcgca gggaggcgat cagaacgcga      120

ccggtggcgc agcccttgct cttgttgctg caggtgtgct tgcggcttgc tcagggggtg      180

ctaagaaaga aggagaagca gctagcaaga aagaaatcat cgttgcaacc aatggatcac      240

caaagccatt tatctatgaa gaaaatggcg aattgactgg ttacgagatt gaagtcgttc      300

gcgctatctt taaagattct gacaaatatg atgtcaagtt tgaaaagaca gaatggtcag      360

gtgtctttgc tggtcttgac gctgatcgtt acaatatggc tgtcaacaat cttagctaca      420

ctaaagaacg tgcggagaaa tacctctatg ccgcaccaat tgcccaaaat cctaatgtcc      480

ttgtcgtgaa gaaagatgac tctagtatca agtctctcga tgatatcggt ggaaaatcga      540

cggaagtcgt tcaagccact acatcagcta agcagttaga agcatacaat gctgaacaca      600

cggacaaccc aactatcctt aactatacta aggcagactt gcaacaaatc atggtacgtt      660

tgagcgatgg acaatttgac tataagattt ttgataaaat cggtgttgaa acagtgatca      720

agaaccaagg tttggacaac ttgaaagtta tcgaacttcc aagcgaccaa caaccgtacg      780

tttacccact tcttgctcag ggtcaagatg agttgaaatc gtttgtagac aaacgcatca      840

aagaacttta taaagatgga actcttgaaa aattgtctaa acaattcttc ggagacactt      900

atctaccggc agaagctgat attaaaggag gcgatcagaa cgcgaccggt ggccatcacc      960

atcatcacca tcatcaccat cattaattaa gtctagagga tcc                       1003


<210>  8
<211>  315
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sp0148 amino acid sequence with sequons,His tag and leader 
       peptide

<400>  8

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Ala Gln Gly Gly Asp Gln Asn Ala Thr Gly Gly Ala Ala 
            20                  25                  30          


Leu Ala Leu Val Ala Ala Gly Val Leu Ala Ala Cys Ser Gly Gly Ala 
        35                  40                  45              


Lys Lys Glu Gly Glu Ala Ala Ser Lys Lys Glu Ile Ile Val Ala Thr 
    50                  55                  60                  


Asn Gly Ser Pro Lys Pro Phe Ile Tyr Glu Glu Asn Gly Glu Leu Thr 
65                  70                  75                  80  


Gly Tyr Glu Ile Glu Val Val Arg Ala Ile Phe Lys Asp Ser Asp Lys 
                85                  90                  95      


Tyr Asp Val Lys Phe Glu Lys Thr Glu Trp Ser Gly Val Phe Ala Gly 
            100                 105                 110         


Leu Asp Ala Asp Arg Tyr Asn Met Ala Val Asn Asn Leu Ser Tyr Thr 
        115                 120                 125             


Lys Glu Arg Ala Glu Lys Tyr Leu Tyr Ala Ala Pro Ile Ala Gln Asn 
    130                 135                 140                 


Pro Asn Val Leu Val Val Lys Lys Asp Asp Ser Ser Ile Lys Ser Leu 
145                 150                 155                 160 


Asp Asp Ile Gly Gly Lys Ser Thr Glu Val Val Gln Ala Thr Thr Ser 
                165                 170                 175     


Ala Lys Gln Leu Glu Ala Tyr Asn Ala Glu His Thr Asp Asn Pro Thr 
            180                 185                 190         


Ile Leu Asn Tyr Thr Lys Ala Asp Leu Gln Gln Ile Met Val Arg Leu 
        195                 200                 205             


Ser Asp Gly Gln Phe Asp Tyr Lys Ile Phe Asp Lys Ile Gly Val Glu 
    210                 215                 220                 


Thr Val Ile Lys Asn Gln Gly Leu Asp Asn Leu Lys Val Ile Glu Leu 
225                 230                 235                 240 


Pro Ser Asp Gln Gln Pro Tyr Val Tyr Pro Leu Leu Ala Gln Gly Gln 
                245                 250                 255     


Asp Glu Leu Lys Ser Phe Val Asp Lys Arg Ile Lys Glu Leu Tyr Lys 
            260                 265                 270         


Asp Gly Thr Leu Glu Lys Leu Ser Lys Gln Phe Phe Gly Asp Thr Tyr 
        275                 280                 285             


Leu Pro Ala Glu Ala Asp Ile Lys Gly Gly Asp Gln Asn Ala Thr Gly 
    290                 295                 300                 


Gly His His His His His His His His His His 
305                 310                 315 


<210>  9
<211>  266
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Sp0148 amino acid sequence

<400>  9

Ala Ala Leu Ala Leu Val Ala Ala Gly Val Leu Ala Ala Cys Ser Gly 
1               5                   10                  15      


Gly Ala Lys Lys Glu Gly Glu Ala Ala Ser Lys Lys Glu Ile Ile Val 
            20                  25                  30          


Ala Thr Asn Gly Ser Pro Lys Pro Phe Ile Tyr Glu Glu Asn Gly Glu 
        35                  40                  45              


Leu Thr Gly Tyr Glu Ile Glu Val Val Arg Ala Ile Phe Lys Asp Ser 
    50                  55                  60                  


Asp Lys Tyr Asp Val Lys Phe Glu Lys Thr Glu Trp Ser Gly Val Phe 
65                  70                  75                  80  


Ala Gly Leu Asp Ala Asp Arg Tyr Asn Met Ala Val Asn Asn Leu Ser 
                85                  90                  95      


Tyr Thr Lys Glu Arg Ala Glu Lys Tyr Leu Tyr Ala Ala Pro Ile Ala 
            100                 105                 110         


Gln Asn Pro Asn Val Leu Val Val Lys Lys Asp Asp Ser Ser Ile Lys 
        115                 120                 125             


Ser Leu Asp Asp Ile Gly Gly Lys Ser Thr Glu Val Val Gln Ala Thr 
    130                 135                 140                 


Thr Ser Ala Lys Gln Leu Glu Ala Tyr Asn Ala Glu His Thr Asp Asn 
145                 150                 155                 160 


Pro Thr Ile Leu Asn Tyr Thr Lys Ala Asp Leu Gln Gln Ile Met Val 
                165                 170                 175     


Arg Leu Ser Asp Gly Gln Phe Asp Tyr Lys Ile Phe Asp Lys Ile Gly 
            180                 185                 190         


Val Glu Thr Val Ile Lys Asn Gln Gly Leu Asp Asn Leu Lys Val Ile 
        195                 200                 205             


Glu Leu Pro Ser Asp Gln Gln Pro Tyr Val Tyr Pro Leu Leu Ala Gln 
    210                 215                 220                 


Gly Gln Asp Glu Leu Lys Ser Phe Val Asp Lys Arg Ile Lys Glu Leu 
225                 230                 235                 240 


Tyr Lys Asp Gly Thr Leu Glu Lys Leu Ser Lys Gln Phe Phe Gly Asp 
                245                 250                 255     


Thr Tyr Leu Pro Ala Glu Ala Asp Ile Lys 
            260                 265     


<210>  10
<211>  713
<212>  PRT
<213>  Campylobacter jejuni PglB amino acid sequence

<400>  10

Met Leu Lys Lys Glu Tyr Leu Lys Asn Pro Tyr Leu Val Leu Phe Ala 
1               5                   10                  15      


Met Ile Ile Leu Ala Tyr Val Phe Ser Val Phe Cys Arg Phe Tyr Trp 
            20                  25                  30          


Val Trp Trp Ala Ser Glu Phe Asn Glu Tyr Phe Phe Asn Asn Gln Leu 
        35                  40                  45              


Met Ile Ile Ser Asn Asp Gly Tyr Ala Phe Ala Glu Gly Ala Arg Asp 
    50                  55                  60                  


Met Ile Ala Gly Phe His Gln Pro Asn Asp Leu Ser Tyr Tyr Gly Ser 
65                  70                  75                  80  


Ser Leu Ser Thr Leu Thr Tyr Trp Leu Tyr Lys Ile Thr Pro Phe Ser 
                85                  90                  95      


Phe Glu Ser Ile Val Leu Tyr Met Ser Thr Phe Leu Ser Ser Leu Val 
            100                 105                 110         


Val Ile Pro Ile Ile Leu Leu Ala Asn Glu Tyr Lys Arg Pro Leu Met 
        115                 120                 125             


Gly Phe Val Ala Ala Leu Leu Ala Ser Ile Ala Asn Ser Tyr Tyr Asn 
    130                 135                 140                 


Arg Thr Met Ser Gly Tyr Tyr Asp Thr Asp Met Leu Val Ile Val Leu 
145                 150                 155                 160 


Pro Met Phe Ile Leu Phe Phe Met Val Arg Met Ile Leu Lys Lys Asp 
                165                 170                 175     


Phe Phe Ser Leu Ile Ala Leu Pro Leu Phe Ile Gly Ile Tyr Leu Trp 
            180                 185                 190         


Trp Tyr Pro Ser Ser Tyr Thr Leu Asn Val Ala Leu Ile Gly Leu Phe 
        195                 200                 205             


Leu Ile Tyr Thr Leu Ile Phe His Arg Lys Glu Lys Ile Phe Tyr Ile 
    210                 215                 220                 


Ala Val Ile Ser Ser Ser Leu Thr Leu Ser Asn Ile Ala Trp Phe Tyr 
225                 230                 235                 240 


Gln Ser Ala Ile Ile Val Ile Leu Phe Ala Leu Phe Ala Leu Glu Gln 
                245                 250                 255     


Lys Arg Leu Asn Phe Met Ile Ile Gly Ile Leu Gly Ser Val Thr Leu 
            260                 265                 270         


Ile Phe Leu Ile Leu Ser Gly Gly Val Asp Pro Ile Leu Tyr Gln Leu 
        275                 280                 285             


Lys Phe Tyr Ile Phe Arg Ser Asp Glu Ser Ala Asn Leu Thr Gln Gly 
    290                 295                 300                 


Phe Met Tyr Phe Asn Val Asn Gln Thr Ile Gln Glu Val Glu Asn Val 
305                 310                 315                 320 


Asp Leu Ser Glu Phe Met Arg Arg Ile Ser Gly Ser Glu Ile Val Phe 
                325                 330                 335     


Leu Phe Ser Leu Phe Gly Phe Ala Trp Leu Leu Arg Lys His Lys Ser 
            340                 345                 350         


Met Ile Met Ala Leu Pro Ile Leu Val Leu Gly Phe Leu Ala Leu Lys 
        355                 360                 365             


Gly Gly Leu Arg Phe Thr Ile Tyr Ser Val Pro Val Met Ala Leu Gly 
    370                 375                 380                 


Phe Gly Phe Leu Leu Ser Glu Phe Lys Ala Ile Leu Val Lys Lys Tyr 
385                 390                 395                 400 


Ser Gln Leu Thr Ser Asn Val Cys Ile Val Phe Ala Thr Ile Leu Thr 
                405                 410                 415     


Leu Ala Pro Val Phe Ile His Ile Tyr Asn Tyr Lys Ala Pro Thr Val 
            420                 425                 430         


Phe Ser Gln Asn Glu Ala Ser Leu Leu Asn Gln Leu Lys Asn Ile Ala 
        435                 440                 445             


Asn Arg Glu Asp Tyr Val Val Thr Trp Trp Asp Tyr Gly Tyr Pro Val 
    450                 455                 460                 


Arg Tyr Tyr Ser Asp Val Lys Thr Leu Val Asp Gly Gly Lys His Leu 
465                 470                 475                 480 


Gly Lys Asp Asn Phe Phe Pro Ser Phe Ala Leu Ser Lys Asp Glu Gln 
                485                 490                 495     


Ala Ala Ala Asn Met Ala Arg Leu Ser Val Glu Tyr Thr Glu Lys Ser 
            500                 505                 510         


Phe Tyr Ala Leu Gln Asn Asp Ile Leu Lys Thr Asp Ile Leu Gln Ala 
        515                 520                 525             


Met Met Lys Asp Tyr Asn Gln Ser Asn Val Asp Leu Phe Leu Ala Ser 
    530                 535                 540                 


Leu Ser Lys Pro Asp Phe Lys Ile Asp Thr Pro Lys Thr Arg Asp Ile 
545                 550                 555                 560 


Tyr Leu Tyr Met Pro Ala Arg Met Ser Leu Ile Phe Ser Thr Val Ala 
                565                 570                 575     


Ser Phe Ala Phe Ile Asn Leu Asp Thr Gly Val Leu Asp Lys Pro Phe 
            580                 585                 590         


Thr Phe Ser Thr Ala Tyr Pro Leu Asp Val Lys Asn Gly Glu Ile Tyr 
        595                 600                 605             


Leu Ser Asn Gly Val Val Leu Ser Asp Asp Phe Arg Ser Phe Lys Ile 
    610                 615                 620                 


Gly Asp Asn Val Val Ser Val Asn Ser Ile Val Glu Ile Asn Ser Ile 
625                 630                 635                 640 


Lys Gln Gly Glu Tyr Lys Ile Thr Pro Ile Asp Asp Lys Ala Gln Phe 
                645                 650                 655     


Tyr Ile Phe Tyr Leu Lys Asp Ser Ala Ile Pro Tyr Ala Gln Phe Ile 
            660                 665                 670         


Leu Met Asp Lys Thr Met Phe Asn Ser Ala Tyr Val Gln Met Phe Phe 
        675                 680                 685             


Leu Gly Asn Tyr Asp Lys Asn Leu Phe Asp Ser Val Ile Asn Ser Arg 
    690                 695                 700                 


Asp Ala Lys Val Phe Lys Leu Lys Ile 
705                 710             


<210>  11
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosylation sequon recognised by Campylobacter jejuni PglB


<220>
<221>  MISC_FEATURE
<222>  (1)..(1)
<223>  Xaa = D or E

<220>
<221>  MISC_FEATURE
<222>  (2)..(2)
<223>  Xaa = Any amino acid except P

<220>
<221>  MISC_FEATURE
<222>  (4)..(4)
<223>  Xaa = Any amino acid except P

<220>
<221>  MISC_FEATURE
<222>  (5)..(5)
<223>  Xaa = S or T

<400>  11

Xaa Xaa Asn Xaa Xaa 
1               5   


<210>  12
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  glycosylation sequon

<400>  12

Asp Gln Asn Ala Thr 
1               5   


<210>  13
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  DsbA leader sequence

<400>  13

Met Lys Lys Ile Trp Leu Ala Leu Ala Gly Leu Val Leu Ala Phe Ser 
1               5                   10                  15      


Ala Ser Ala Ala Gln 
            20      


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  14

Met Arg Gly Ser His His His His His His 
1               5                   10  


<210>  15
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  15

Asp Tyr Lys Asp Asp Asp Asp Lys 
1               5               


<210>  16
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  16

Lys Glu Thr Ala Ala Ala Lys Phe Glu Arg Gln His Met Asp Ser 
1               5                   10                  15  


<210>  17
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  17

Arg Arg Arg Arg Arg 
1               5   


<210>  18
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  18

Arg Arg Arg Arg Arg Arg 
1               5       


<210>  19
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  19

His His His His His His 
1               5       


<210>  20
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  20

His His His His His His His 
1               5           


<210>  21
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  21

His His His His His His His His 
1               5               


<210>  22
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  22

His His His His His His His His His 
1               5                   


<210>  23
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  23

His His His His His His His His His His 
1               5                   10  


<210>  24
<211>  4
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  24

Cys Cys Cys Cys 
1               


<210>  25
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  25

Phe Phe Phe Phe Phe Phe Phe Phe Phe Phe Phe 
1               5                   10      


<210>  26
<211>  5
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  26

Asp Asp Asp Asp Asp 
1               5   


<210>  27
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  27

Asp Asp Asp Asp Asp Asp 
1               5       


<210>  28
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  28

Asp Asp Asp Asp Asp Asp Asp 
1               5           


<210>  29
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  29

Asp Asp Asp Asp Asp Asp Asp Asp 
1               5               


<210>  30
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  30

Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   


<210>  31
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  31

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10  


<210>  32
<211>  11
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  32

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10      


<210>  33
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  33

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10          


<210>  34
<211>  13
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  34

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10              


<210>  35
<211>  14
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  35

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10                  


<210>  36
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  36

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10                  15  


<210>  37
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  purification tag

<400>  37

Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp Asp 
1               5                   10                  15      


<210>  38
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  strep-tag II

<400>  38

Trp Ser His Pro Gln Phe Glu Lys 
1               5               


<210>  39
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  c-myc tag

<400>  39

Glu Gln Lys Leu Ile Ser Glu Glu Asp Leu 
1               5                   10  


<210>  40
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cruz tag 09

<400>  40

Met Lys Ala Glu Phe Arg Arg Gln Glu Ser Asp Arg 
1               5                   10          


<210>  41
<211>  12
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Cruz tag 22

<400>  41

Met Arg Asp Ala Leu Asp Arg Leu Asp Arg Leu Ala 
1               5                   10          


