                         SEQUENCE LISTING

<110>  University of Newcastle upon Tyne
 
<120>  Biomarker

<130>  P226294WO

<140>  PCT/GB2019/051240
<141>  2019-05-03

<150>  GB 1807367.6
<151>  2018-05-04

<160>  49    

<170>  PatentIn version 3.5

<210>  1
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide biomarker for identification of Clostridium difficile

<400>  1

Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu Ala 
1               5                   10                  15      


<210>  2
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  epitope to which an antibody/ antigen binding fragment binds

<400>  2

Lys Glu Asp Gln Leu Val Asp Ala 
1               5               


<210>  3
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence to which antibody or antigen binding fragment binds

<400>  3

Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
1               5                   10  


<210>  4
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  peptide sequence to which the antibody or antigen binding 
       fragment binds

<400>  4

Lys Glu Asp Gln Leu Val Asp Ala Leu Ala 
1               5                   10  


<210>  5
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  heavy chain variable domain binding to SEQ ID NO:1

<400>  5

Val Gln Leu Leu Glu Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Tyr 
            20                  25                  30          


Met His Trp Val Arg Gln Ser His Val Lys Ser Leu Glu Trp Ile Gly 
        35                  40                  45              


Arg Ile Asn Pro Tyr Asn Gly Ala Thr Arg Tyr Asn Gln Asn Phe Lys 
    50                  55                  60                  


Asp Lys Ala Ser Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met 
65                  70                  75                  80  


Glu Leu His Ser Leu Thr Ser Glu Asp Ser Ser Val Tyr Tyr Cys Thr 
                85                  90                  95      


Val Ser Leu Asp Tyr Trp Gly Gln Gly Thr Ser Leu Thr Val Ser Ser 
            100                 105                 110         


<210>  6
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  light chain variable domain binding to SEQ ID NO: 1

<400>  6

Glu Leu Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Thr 
            20                  25                  30          


Asn Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Met Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  7
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR1

<400>  7

Gly Tyr Ser Phe Thr Gly Tyr Tyr Met His 
1               5                   10  


<210>  8
<211>  15
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR2

<400>  8

Arg Ile Asn Pro Tyr Asn Gly Ala Thr Arg Tyr Asn Gln Asn Phe 
1               5                   10                  15  


<210>  9
<211>  6
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VH CDR3

<400>  9

Thr Val Ser Leu Asp Tyr 
1               5       


<210>  10
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR1

<400>  10

Lys Ser Ser Gln Ser Leu Leu Asp Thr Asn Gly Lys Thr Tyr Leu Asn 
1               5                   10                  15      


<210>  11
<211>  7
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR2

<400>  11

Leu Val Ser Lys Met Asp Ser 
1               5           


<210>  12
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  VL CDR3

<400>  12

Trp Gln Gly Thr His Phe Pro Tyr Thr 
1               5                   


<210>  13
<211>  16
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  C. difficile HMW SLP biomarker sequence

<400>  13

Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu Ala 
1               5                   10                  15      


<210>  14
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab370

<400>  14

Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu 
1               5                   10  


<210>  15
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab521

<400>  15

Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
1               5                   10  


<210>  16
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab498

<400>  16

Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
1               5                   10  


<210>  17
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab491

<400>  17

Phe Val Ala Lys Asp Gly Ser Thr Lys Glu 
1               5                   10  


<210>  18
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab652

<400>  18

Lys Glu Asp Gln Thr Val Asp Ala Leu Ala 
1               5                   10  


<210>  19
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab493

<400>  19

Val Asp Ala Leu Ala Ala Ala Pro Ile Ala 
1               5                   10  


<210>  20
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AbB31

<400>  20

Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
1               5                   10  


<210>  21
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  AbB84

<400>  21

Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
1               5                   10  


<210>  22
<211>  10
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ab652

<400>  22

Lys Glu Asp Gln Leu Val Asp Ala Leu Ala 
1               5                   10  


<210>  23
<211>  20
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Forward SlpA

<400>  23

Ala Thr Gly Ala Gly Thr Ala Thr Ala Gly Cys Thr Cys Cys Ala Gly 
1               5                   10                  15      


Thr Thr Gly Cys 
            20  


<210>  24
<211>  21
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  REVERSE SLPA

<400>  24

Ala Thr Cys Thr Thr Cys Ala Thr Cys Ala Cys Cys Ala Thr Cys Thr 
1               5                   10                  15      


Cys Cys Thr Gly Cys 
            20      


<210>  25
<211>  339
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variable heavy domain

<400>  25
taggtccagc tgctcgagtc tggacctgag ctggtgaagc ctggggcttc agtgaagatc       60

tcctgcaagg cttctggtta ctcattcact ggctactaca tgcactgggt taggcaaagc      120

catgtaaaga gccttgagtg gattggacgt attaatcctt acaatggtgc tactaggtac      180

aaccagaatt tcaaggacaa ggccagcttg actgtagata agtcctccag cacagcctac      240

atggagctcc acagcctgac atctgaggac tcttcagtct attactgtac agtaagcctc      300

gactactggg gccaaggcac cagtctcaca gtctcctca                             339


<210>  26
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variable heavy domain

<400>  26

Val Gln Leu Leu Glu Ser Gly Pro Glu Leu Val Lys Pro Gly Ala Ser 
1               5                   10                  15      


Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr Tyr 
            20                  25                  30          


Met His Trp Val Arg Gln Ser His Val Lys Ser Leu Glu Trp Ile Gly 
        35                  40                  45              


Arg Ile Asn Pro Tyr Asn Gly Ala Thr Arg Tyr Asn Gln Asn Phe Lys 
    50                  55                  60                  


Asp Lys Ala Ser Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr Met 
65                  70                  75                  80  


Glu Leu His Ser Leu Thr Ser Glu Asp Ser Ser Val Tyr Tyr Cys Thr 
                85                  90                  95      


Val Ser Leu Asp Tyr Trp Gly Gln Gly Thr Ser Leu Thr Val Ser Ser 
            100                 105                 110         


<210>  27
<211>  336
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  variable light domain

<400>  27
gagctcgtga tgacccagac tccactcact ttgtcggtta ccattggaca accagcctcc       60

atctcttgca agtcaagtca gagcctctta gatactaatg gaaagacata tttgaattgg      120

ttgttacaga ggccaggcca gtctccaaag cgcctaatct atctggtgtc caaaatggac      180

tctggagtcc ctgacaggtt cactggcagt ggatcaggga cagatttcac actgaaaatc      240

agcagagtgg aggctgagga tttgggagtt tattattgct ggcaaggtac acattttccg      300

tacacgttcg gaggggggac caagctggaa ataaaa                                336


<210>  28
<211>  112
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  variable light domain

<400>  28

Glu Leu Val Met Thr Gln Thr Pro Leu Thr Leu Ser Val Thr Ile Gly 
1               5                   10                  15      


Gln Pro Ala Ser Ile Ser Cys Lys Ser Ser Gln Ser Leu Leu Asp Thr 
            20                  25                  30          


Asn Gly Lys Thr Tyr Leu Asn Trp Leu Leu Gln Arg Pro Gly Gln Ser 
        35                  40                  45              


Pro Lys Arg Leu Ile Tyr Leu Val Ser Lys Met Asp Ser Gly Val Pro 
    50                  55                  60                  


Asp Arg Phe Thr Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Lys Ile 
65                  70                  75                  80  


Ser Arg Val Glu Ala Glu Asp Leu Gly Val Tyr Tyr Cys Trp Gln Gly 
                85                  90                  95      


Thr His Phe Pro Tyr Thr Phe Gly Gly Gly Thr Lys Leu Glu Ile Lys 
            100                 105                 110         


<210>  29
<211>  60
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  Ox1121

<400>  29

Val Ile Asn Glu Tyr Phe Lys Lys Glu Asn Lys Thr Val Lys Asn Phe 
1               5                   10                  15      


Phe Leu Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
            20                  25                  30          


Leu Ala Ser Ala Ala Val Ala Gly Asn Phe Gly Ser Val Asn Gly Asn 
        35                  40                  45              


Gln Asp Lys Val Ser Pro Ala Pro Ile Val Leu Ala 
    50                  55                  60  


<210>  30
<211>  59
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1342

<400>  30

Val Ile Lys Glu Tyr Tyr Glu Asn Asp Pro Lys Asn Val Lys Asn Ile 
1               5                   10                  15      


Phe Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
            20                  25                  30          


Leu Ala Ala Gly Ala Ile Ala Gly Asn Leu Gly Leu Ser Ala Gly Glu 
        35                  40                  45              


Asp Glu Val Ser Pro Ala Pro Ile Val Leu Ala 
    50                  55                  


<210>  31
<211>  55
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1192c

<400>  31

Val Ile Lys Thr Tyr Phe Glu Lys Asp Asn Ser Asp Ser Val Ile Ser 
1               5                   10                  15      


Thr Gly Val Lys Asn Phe Tyr Val Ala Lys Asp Gly Ser Thr Lys Glu 
            20                  25                  30          


Asp Gln Leu Val Asp Ala Leu Ala Ile Ala Ala Val Ala Gly His Asn 
        35                  40                  45              


Glu Ala Pro Ile Val Leu Ala 
    50                  55  


<210>  32
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1523

<400>  32

Val Ile Lys Thr Tyr Tyr Ala Asn Asp Thr Glu Ile Ala Lys Ala Ala 
1               5                   10                  15      


Val Leu Asp Lys Asp Ser Gly Ala Ser Ser Ser Asp Ala Gly Val Phe 
            20                  25                  30          


Asn Phe Tyr Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val 
        35                  40                  45              


Asp Ala Leu Ala Val Gly Ala Val Ala Gly Tyr Lys Leu Ala Pro Val 
    50                  55                  60                  


Val Leu Ala 
65          


<210>  33
<211>  62
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1533

<400>  33

Val Ile Lys Glu Tyr Phe Asp Lys Asp Gly Val Ser Asn Tyr Phe Leu 
1               5                   10                  15      


Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu Ala 
            20                  25                  30          


Ala Ala Ala Val Ala Gly Asn Tyr Gly Ser Lys His Asn Glu Asp Gly 
        35                  40                  45              


Asp Ile Thr Thr Asp Ala Ser Pro Ala Pro Ile Ile Leu Ala 
    50                  55                  60          


<210>  34
<211>  67
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1424

<400>  34

Val Ile Lys Glu Asp Asp Phe Phe Lys Asn Gly Glu Val Thr Asn Phe 
1               5                   10                  15      


Phe Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
            20                  25                  30          


Leu Ala Gly Ala Ala Ile Ala Gly Asn Phe Gly Val Thr Val Asp Asn 
        35                  40                  45              


Glu Gly Lys Pro Thr Val Ala Asp Lys Lys Ala Ser Pro Ala Pro Ile 
    50                  55                  60                  


Val Leu Ala 
65          


<210>  35
<211>  84
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox160

<400>  35

Val Ile Lys Glu Ser Ser Tyr Tyr Gln Asp Asn Leu Asn Asn Asp Lys 
1               5                   10                  15      


Lys Val Val Asn Phe Phe Val Ala Lys Asp Gly Ser Thr Lys Glu Asp 
            20                  25                  30          


Gln Leu Val Asp Ala Leu Ala Ala Ala Pro Val Ala Ala Asn Phe Gly 
        35                  40                  45              


Val Thr Leu Asn Ser Asp Gly Lys Pro Val Asp Lys Asp Gly Lys Val 
    50                  55                  60                  


Leu Thr Gly Ser Asp Asn Asp Lys Asn Lys Leu Val Ser Pro Ala Pro 
65                  70                  75                  80  


Ile Val Leu Ala 
                


<210>  36
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1437a

<400>  36

Val Leu Lys Glu Ser Asp Tyr Phe Pro Asp Gly Ala Val Asn Tyr Phe 
1               5                   10                  15      


Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu 
            20                  25                  30          


Ala Ala Ala Pro Val Ala Ala His Phe Glu Ala Pro Ile Ile Leu Ala 
        35                  40                  45              


<210>  37
<211>  64
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1896

<400>  37

Val Leu Lys Glu Ser Asp Tyr Phe Pro Asp Gly Ala Val Asn Tyr Phe 
1               5                   10                  15      


Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu 
            20                  25                  30          


Ala Ala Ala Pro Val Ala Ala Asn Phe Gly Arg Thr Tyr Asn Ile Lys 
        35                  40                  45              


Asp Asn Asp Ser Ser Gly Thr Val Ser Pro Ala Pro Ile Ile Leu Ala 
    50                  55                  60                  


<210>  38
<211>  64
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox575

<400>  38

Val Leu Lys Glu Ser Asp Tyr Phe Pro Asp Gly Ala Val Asn Tyr Phe 
1               5                   10                  15      


Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu 
            20                  25                  30          


Ala Ala Ala Pro Val Ala Ala Asn Phe Gly Arg Thr Tyr Asn Ile Lys 
        35                  40                  45              


Asp Asn Asp Ser Ser Gly Thr Val Ser Pro Ala Pro Ile Ile Leu Ala 
    50                  55                  60                  


<210>  39
<211>  61
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1396

<400>  39

Val Ile Lys Asn Ser Asp Tyr Tyr Glu Lys Gly Thr Val Lys Asn Phe 
1               5                   10                  15      


Phe Leu Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
            20                  25                  30          


Leu Ala Ala Ala Ala Val Ala Gly Asn Phe Gly Thr Lys Phe Asp Ala 
        35                  40                  45              


Ala Gly Gly Glu Thr Ser Pro Ala Pro Ile Val Leu Ala 
    50                  55                  60      


<210>  40
<211>  63
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox858

<400>  40

Val Met Lys Glu Thr Asp Tyr Phe Glu Lys Gly Ser Val Ile Asn Tyr 
1               5                   10                  15      


Phe Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
            20                  25                  30          


Leu Ala Ala Ala Pro Val Ala Ala Asn Phe Gly Ser Thr Tyr Asp Gly 
        35                  40                  45              


Lys Asn Ala Asn Gly Thr Val Ser Pro Ala Pro Ile Val Leu Ala 
    50                  55                  60              


<210>  41
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  ox1145

<400>  41

Val Leu Lys Glu Asp Asp Tyr Phe Thr Asp Gly Glu Val Val Asn Tyr 
1               5                   10                  15      


Phe Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
            20                  25                  30          


Leu Ala Ala Ala Pro Ile Ala Gly Arg Phe Lys Glu Ser Pro Ala Pro 
        35                  40                  45              


Ile Ile Leu Ala 
    50          


<210>  42
<211>  52
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  630

<400>  42

Val Leu Lys Glu Asp Asp Tyr Phe Thr Asp Gly Glu Val Val Asn Tyr 
1               5                   10                  15      


Phe Val Ala Lys Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala 
            20                  25                  30          


Leu Ala Ala Ala Pro Ile Ala Gly Arg Phe Lys Glu Ser Pro Ala Pro 
        35                  40                  45              


Ile Ile Leu Ala 
    50          


<210>  43
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  r002

<400>  43

Glu Ser Asp Tyr Phe Pro Asp Gly Ala Val Asn Tyr Phe Val Ala Lys 
1               5                   10                  15      


Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu Ala Ala Ala 
            20                  25                  30          


Pro Val Ala Ala Asn Phe Gly Arg Thr Tyr Asn Ile Lys Asp 
        35                  40                  45      


<210>  44
<211>  46
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  R050

<400>  44

Glu Ser Asp Tyr Phe Pro Asp Gly Ala Val Asn Tyr Phe Val Ala Lys 
1               5                   10                  15      


Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu Ala Ala Ala 
            20                  25                  30          


Pro Val Ala Ala Asn Phe Gly Arg Thr Tyr Asn Ile Lys Asp 
        35                  40                  45      


<210>  45
<211>  48
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  630

<400>  45

Asp Asp Tyr Phe Thr Asp Gly Glu Val Glu Asn Tyr Phe Val Ala Lys 
1               5                   10                  15      


Asp Gly Ser Thr Lys Glu Asp Gln Leu Val Asp Ala Leu Ala Ala Ala 
            20                  25                  30          


Pro Ile Ala Gly Arg Phe Lys Glu Ser Pro Ala Pro Ile Ile Leu Ala 
        35                  40                  45              


<210>  46
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence in Fig18

<400>  46

Asp Ala Leu Ala Ala Ala Pro Ile Ala 
1               5                   


<210>  47
<211>  8
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequence in Fig18

<400>  47

Asp Ala Leu Ala Ala Ala Pro Ala 
1               5               


<210>  48
<211>  9
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sbjct 234

<400>  48

Asp Ala Leu Ala Ala Ala Pro Leu Ala 
1               5                   


<210>  49
<211>  113
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  sequnce in Fig34

<400>  49

Cys Val Glu Leu Leu Glu Ser Gly Pro Glu Leu Val Lys Pro Gly Ala 
1               5                   10                  15      


Ser Val Lys Ile Ser Cys Lys Ala Ser Gly Tyr Ser Phe Thr Gly Tyr 
            20                  25                  30          


Tyr Met His Trp Val Arg Gln Ser His Val Lys Ser Leu Glu Trp Ile 
        35                  40                  45              


Gly Arg Ile Asn Pro Tyr Asn Gly Ala Thr Arg Tyr Asn Gln Asn Phe 
    50                  55                  60                  


Lys Asp Lys Ala Ser Leu Thr Val Asp Lys Ser Ser Ser Thr Ala Tyr 
65                  70                  75                  80  


Met Glu Leu His Ser Leu Thr Ser Glu Asp Ser Ser Val Tyr Tyr Cys 
                85                  90                  95      


Thr Val Ser Leu Asp Tyr Trp Gly Gln Gly Thr Ser Leu Thr Val Ser 
            100                 105                 110         


Ser 
    


