                         

<110>  Ambrx, Inc
 
<120>  A Method For Optimizing Antibody Expression

<130>  AMBX-0226.00PCT

<150>  62/665,093
<151>  2018-05-01

<160>  18    

<170>  PatentIn version 3.5

<210>  1
<211>  23
<212>  PRT
<213>  E. Coli

<400>  1

Met Lys Lys Asn Ile Ala Phe Leu Leu Ala Ser Met Phe Val Phe Ser 
1               5                   10                  15      


Ile Ala Thr Asn Ala Tyr Ala 
            20              


<210>  2
<211>  110
<212>  PRT
<213>  E. Coli

<400>  2

Gln Ala Val Val Thr Gln Glu Pro Ser Leu Thr Val Ser Pro Gly Gly 
1               5                   10                  15      


Thr Val Thr Leu Thr Cys Arg Ser Ser Thr Gly Ala Val Thr Thr Ser 
            20                  25                  30          


Asn Tyr Ala Asn Trp Val Gln Gln Lys Pro Gly Gln Ala Pro Arg Gly 
        35                  40                  45              


Leu Ile Gly Gly Thr Asn Lys Arg Ala Pro Gly Thr Pro Ala Arg Phe 
    50                  55                  60                  


Ser Gly Ser Leu Leu Gly Gly Lys Ala Ala Leu Thr Leu Ser Gly Ala 
65                  70                  75                  80  


Gln Pro Glu Asp Glu Ala Glu Tyr Tyr Cys Ala Leu Trp Tyr Ser Asn 
                85                  90                  95      


Leu Trp Val Phe Gly Gly Gly Thr Lys Leu Thr Val Leu Gly 
            100                 105                 110 


<210>  3
<211>  107
<212>  PRT
<213>  E. Coli

<400>  3

Arg Thr Val Ala Ala Pro Ser Val Phe Ile Phe Pro Pro Ser Asp Glu 
1               5                   10                  15      


Gln Leu Lys Ser Gly Thr Ala Ser Val Val Cys Leu Leu Asn Asn Phe 
            20                  25                  30          


Tyr Pro Arg Glu Ala Lys Val Gln Trp Lys Val Asp Asn Ala Leu Gln 
        35                  40                  45              


Ser Gly Asn Ser Gln Glu Ser Val Thr Glu Gln Asp Ser Lys Asp Ser 
    50                  55                  60                  


Thr Tyr Ser Leu Ser Ser Thr Leu Thr Leu Ser Lys Ala Asp Tyr Glu 
65                  70                  75                  80  


Lys His Lys Val Tyr Ala Cys Glu Val Thr His Gln Gly Leu Ser Ser 
                85                  90                  95      


Pro Val Thr Lys Ser Phe Asn Arg Gly Glu Cys 
            100                 105         


<210>  4
<211>  125
<212>  PRT
<213>  E. Coli

<400>  4

Glu Val Gln Leu Val Glu Ser Gly Gly Gly Leu Val Gln Pro Gly Gly 
1               5                   10                  15      


Ser Leu Arg Leu Ser Cys Ala Ala Ser Gly Phe Thr Phe Asn Thr Tyr 
            20                  25                  30          


Ala Met Asn Trp Val Arg Gln Ala Pro Gly Lys Gly Leu Glu Trp Val 
        35                  40                  45              


Ala Arg Ile Arg Ser Lys Tyr Asn Asn Tyr Ala Thr Tyr Tyr Ala Asp 
    50                  55                  60                  


Ser Val Lys Asp Arg Phe Thr Ile Ser Arg Asp Asp Ser Lys Asn Ile 
65                  70                  75                  80  


Leu Tyr Leu Gln Met Asn Ser Leu Lys Thr Glu Asp Thr Ala Val Tyr 
                85                  90                  95      


Tyr Cys Val Arg His Gly Asn Phe Gly Asn Ser Tyr Val Ser Trp Phe 
            100                 105                 110         


Ala Tyr Trp Gly Gln Gly Thr Leu Val Thr Val Ser Ser 
        115                 120                 125 


<210>  5
<211>  103
<212>  PRT
<213>  E. Coli


<220>
<221>  pAcF
<222>  (16)..(16)
<223>  Unnatural amino acid

<400>  5

Ala Ser Thr Lys Gly Pro Ser Val Phe Pro Leu Ala Pro Ser Ser Xaa 
1               5                   10                  15      


Ser Thr Ser Gly Gly Thr Ala Ala Leu Gly Cys Leu Val Lys Asp Tyr 
            20                  25                  30          


Phe Pro Glu Pro Val Thr Val Ser Trp Asn Ser Gly Ala Leu Thr Ser 
        35                  40                  45              


Gly Val His Thr Phe Pro Ala Val Leu Gln Ser Ser Gly Leu Tyr Ser 
    50                  55                  60                  


Leu Ser Ser Val Val Thr Val Pro Ser Ser Ser Leu Gly Thr Gln Thr 
65                  70                  75                  80  


Tyr Ile Cys Asn Val Asn His Lys Pro Ser Asn Thr Lys Val Asp Lys 
                85                  90                  95      


Lys Val Glu Pro Lys Ser Cys 
            100             


<210>  6
<211>  69
<212>  DNA
<213>  E. Coli

<400>  6
atgaaaaaga atatcgcatt tcttcttgca tctatgttcg ttttttctat tgctacaaac       60

gcgtacgct                                                               69


<210>  7
<211>  330
<212>  DNA
<213>  E.Coli

<400>  7
caggctgtgg tgactcagga gccctcactg actgtgtccc caggagggac agtcactctc       60

acctgtcgct ccagcactgg agctgtcacc actagtaatt atgccaactg ggtccagcag      120

aagcctggcc aagcccccag gggactgatt ggtgggacaa acaagagagc tcccgggaca      180

cctgcccggt tctcaggctc cctccttggg ggcaaagctg ccctgaccct ttcgggtgcg      240

cagcctgagg atgaggctga gtattactgc gctctctggt atagtaatct gtgggtgttc      300

ggcggaggga ccaagttgac cgtcctcgga                                       330


<210>  8
<211>  321
<212>  DNA
<213>  E.Coli

<400>  8
cgtacggtgg ctgcaccatc tgtcttcatc ttcccgccat ctgatgagca gttgaaatct       60

ggaactgcct ctgttgtgtg cctgctgaat aacttctatc ccagagaggc caaagtacag      120

tggaaggtgg ataacgccct ccaatcgggt aactcccagg agagtgtcac agagcaggac      180

agcaaggaca gcacctacag cctcagcagc accctgacgc tgagcaaagc agactacgag      240

aaacacaaag tctacgcctg cgaagtcacc catcagggcc tgagctcgcc cgtcacaaag      300

agcttcaaca ggggagagtg t                                                321


<210>  9
<211>  32
<212>  DNA
<213>  E. Coli

<400>  9
tctggggata agaattcggt tgaggtgatt tt                                     32


<210>  10
<211>  375
<212>  DNA
<213>  E. Coli

<400>  10
gaggtgcagc tggtggagtc tgggggaggc ttggtccagc ctggagggtc cctgagactc       60

tcctgtgcag cctctggatt caccttcaat acctacgcaa tgaactgggt ccgccaggct      120

ccagggaagg ggctggagtg ggttgctcgt attagaagca aatataacaa ttacgccaca      180

tattacgccg attctgtgaa agacagattc accatctcaa gagatgattc aaagaacatt      240

ctgtatctgc aaatgaacag cctgaaaacc gaggacacgg ccgtgtatta ctgtgttaga      300

catgggaatt tcggcaactc ttatgtcagt tggtttgcct actggggcca aggtaccctg      360

gtcaccgtct cgagt                                                       375


<210>  11
<211>  309
<212>  DNA
<213>  E. Coli

<400>  11
gctagcacca agggcccatc ggtcttcccc ctggcaccct cctcctagag cacctctggg       60

ggcacagcgg ccctgggctg cctggtcaag gactacttcc ccgaaccggt gacggtgtcg      120

tggaactcag gcgccctgac cagcggcgtg cacaccttcc cggctgtcct acagtcctca      180

ggactctact ccctcagcag cgtggtgacc gtgccctcca gcagcttggg cacccagacc      240

tacatctgca acgtgaatca caagcccagc aacaccaagg tggacaagaa agttgagccc      300

aaatcttgt                                                              309


<210>  12
<211>  580
<212>  DNA
<213>  E. Coli

<400>  12
aacaaactcc gggaggcagc gtgatgcggc aacaatcaca cggatttccc gtgaacggtc       60

tgaatgagcg gattattttc agggaaagtg agtgtggtca gcgtgcaggt atatgggcta      120

tgatgtgccc ggcgcttgag gctttctgcc tcatgacgtg aaggtggttt gttgccgtgt      180

tgtgtggcag aaagaagata gccccgtagt aagttaattt tcattaacca ccacgaggca      240

tccctatgtc tagtccacat caggatagcc tcttaccgcg ctttgcgcaa ggagaagaag      300

gccatgaaac taccacgaag ttcccttgtc tggtgtgtgt tgatcgtgtg tctcacactg      360

ttgatattca cttatctgac acgaaaatcg ctgtgcgaga ttcgttacag agacggacac      420

agggaggtgg cggctttcat ggcttacgaa tccggtaagt agcaacctgg aggcgggcgc      480

aggcccgcct tttcaggact gatgctggtc tgactactga agcgccttta taaaggggct      540

gctggttcgc cggtagcccc tttctccttg ctgatgttgt                            580


<210>  13
<211>  156
<212>  DNA
<213>  E. Coli

<400>  13
atgaaactac cacgaagttc ccttgtctgg tgtgtgttga tcgtgtgtct cacactgttg       60

atattcactt atctgacacg aaaatcgctg tgcgagattc gttacagaga cggacacagg      120

gaggtggcgg ctttcatggc ttacgaatcc ggtaag                                156


<210>  14
<211>  52
<212>  PRT
<213>  E. Coli

<400>  14

Met Lys Leu Pro Arg Ser Ser Leu Val Trp Cys Val Leu Ile Val Cys 
1               5                   10                  15      


Leu Thr Leu Leu Ile Phe Thr Tyr Leu Thr Arg Lys Ser Leu Cys Glu 
            20                  25                  30          


Ile Arg Tyr Arg Asp Gly His Arg Glu Val Ala Ala Phe Met Ala Tyr 
        35                  40                  45              


Glu Ser Gly Lys 
    50          


<210>  15
<211>  1199
<212>  DNA
<213>  E. Coli

<400>  15
gattcacctc ttttgtcgaa tggtcgccgc ggattctact taactttgct gcccgagaca       60

gcactcattt cgcggtcatc gaaactaatt taaacaaaaa gagtctgaaa atagatgata      120

atagggcgtg tctgtatgta gatttgttcg acaacgcttt atagtaccct tctgataata      180

gttaaccctg gggtgagatg ccccgatcct ggagatatgg atgaaatcac tgtttaaagt      240

aacgctgctg gcgaccacaa tggccgttgc cctgcatgca ccaatcactt ttgctgctga      300

agctgcaaaa cctgctacag ctgctgacag caaagcagcg ttcaaaaatg acgatcagaa      360

atcagcttat gcactgggtg cctcgctggg tcgttacatg gaaaactctc taaaagaaca      420

agaaaaactg ggcatcaaac tggataaaga tcagctgatc gctggtgttc aggatgcatt      480

tgctgataag agcaaactct ccgaccaaga gatcgaacag actctacaag cattcgaagc      540

tcgcgtgaag tcttctgctc aggcgaagat ggaaaaagac gcggctgata acgaagcaaa      600

aggtaaagag taccgcgaga aatttgccaa agagaaaggt gtgaaaacct cttcaactgg      660

tctggtttat caggtagtag aagccggtaa aggcgaagca ccgaaagaca gcgatactgt      720

tgtagtgaac tacaaaggta cgctgatcga cggtaaagag ttcgacaact cttacacccg      780

tggtgaaccg ctttctttcc gtctggacgg tgttatcccg ggttggacag aaggtctgaa      840

gaacatcaag aaaggcggta agatcaaact ggttattcca ccagaactgg cttacggcaa      900

agcgggtgtt ccggggatcc caccgaattc taccctggtg tttgacgtag agctgctgga      960

tgtgaaacca gcgccgaagg ctgatgcaaa gccggaagct gatgcgaaag ccgcagattc     1020

tgctaaaaaa taagcattaa gaaccgccgc ctgaccaggc ggcggttttt ttattacagg     1080

ccggatataa ttagtgctgg aaagcggaac ctccgctgta ttaatttagt tacccgcatc     1140

attaatgagc ctgccctgaa aagttaacga caggctcctg aaaaggagtg ttttttttc      1199


<210>  16
<211>  270
<212>  PRT
<213>  E. Coli

<400>  16

Met Lys Ser Leu Phe Lys Val Thr Leu Leu Ala Thr Thr Met Ala Val 
1               5                   10                  15      


Ala Leu His Ala Pro Ile Thr Phe Ala Ala Glu Ala Ala Lys Pro Ala 
            20                  25                  30          


Thr Ala Ala Asp Ser Lys Ala Ala Phe Lys Asn Asp Asp Gln Lys Ser 
        35                  40                  45              


Ala Tyr Ala Leu Gly Ala Ser Leu Gly Arg Tyr Met Glu Asn Ser Leu 
    50                  55                  60                  


Lys Glu Gln Glu Lys Leu Gly Ile Lys Leu Asp Lys Asp Gln Leu Ile 
65                  70                  75                  80  


Ala Gly Val Gln Asp Ala Phe Ala Asp Lys Ser Lys Leu Ser Asp Gln 
                85                  90                  95      


Glu Ile Glu Gln Thr Leu Gln Ala Phe Glu Ala Arg Val Lys Ser Ser 
            100                 105                 110         


Ala Gln Ala Lys Met Glu Lys Asp Ala Ala Asp Asn Glu Ala Lys Gly 
        115                 120                 125             


Lys Glu Tyr Arg Glu Lys Phe Ala Lys Glu Lys Gly Val Lys Thr Ser 
    130                 135                 140                 


Ser Thr Gly Leu Val Tyr Gln Val Val Glu Ala Gly Lys Gly Glu Ala 
145                 150                 155                 160 


Pro Lys Asp Ser Asp Thr Val Val Val Asn Tyr Lys Gly Thr Leu Ile 
                165                 170                 175     


Asp Gly Lys Glu Phe Asp Asn Ser Tyr Thr Arg Gly Glu Pro Leu Ser 
            180                 185                 190         


Phe Arg Leu Asp Gly Val Ile Pro Gly Trp Thr Glu Gly Leu Lys Asn 
        195                 200                 205             


Ile Lys Lys Gly Gly Lys Ile Lys Leu Val Ile Pro Pro Glu Leu Ala 
    210                 215                 220                 


Tyr Gly Lys Ala Gly Val Pro Gly Ile Pro Pro Asn Ser Thr Leu Val 
225                 230                 235                 240 


Phe Asp Val Glu Leu Leu Asp Val Lys Pro Ala Pro Lys Ala Asp Ala 
                245                 250                 255     


Lys Pro Glu Ala Asp Ala Lys Ala Ala Asp Ser Ala Lys Lys 
            260                 265                 270 


<210>  17
<211>  634
<212>  DNA
<213>  E. Coli

<400>  17
tttaacatcg gtaaaacctg gtaagtgttc tccacaaagg aatgtagtgg tagtgtagcg       60

atgactttag gcgatcaata taagatcgcc gggccacgca aagaactgca ccctccggtg      120

caaatgggat ggtaaggagt ttattgtgaa aaagtggtta ttagctgcag gtctcggttt      180

agcactggca acttctgctc aggcggctga caaaattgca atcgtcaaca tgggcagcct      240

gttccagcag gtagcgcaga aaaccggtgt ttctaacacg ctggaaaatg agttcaaagg      300

ccgtgccagc gaactgcagc gtatggaaac cgatctgcag gctaaaatga aaaagctgca      360

gtccatgaaa gcgggcagcg atcgcactaa gctggaaaaa gacgtgatgg ctcagcgcca      420

gacttttgct cagaaagcgc aggcttttga gcaggatcgc gcacgtcgtt ccaacgaaga      480

acgcggcaaa ctggttactc gtatccagac tgctgtgaaa tccgttgcca acagccagga      540

tatcgatctg gttgttgatg caaacgccgt tgcttacaac agcagcgatg taaaagacat      600

cactgccgac gtactgaaac aggttaaata agta                                  634


<210>  18
<211>  161
<212>  PRT
<213>  E. Coli

<400>  18

Met Lys Lys Trp Leu Leu Ala Ala Gly Leu Gly Leu Ala Leu Ala Thr 
1               5                   10                  15      


Ser Ala Gln Ala Ala Asp Lys Ile Ala Ile Val Asn Met Gly Ser Leu 
            20                  25                  30          


Phe Gln Gln Val Ala Gln Lys Thr Gly Val Ser Asn Thr Leu Glu Asn 
        35                  40                  45              


Glu Phe Lys Gly Arg Ala Ser Glu Leu Gln Arg Met Glu Thr Asp Leu 
    50                  55                  60                  


Gln Ala Lys Met Lys Lys Leu Gln Ser Met Lys Ala Gly Ser Asp Arg 
65                  70                  75                  80  


Thr Lys Leu Glu Lys Asp Val Met Ala Gln Arg Gln Thr Phe Ala Gln 
                85                  90                  95      


Lys Ala Gln Ala Phe Glu Gln Asp Arg Ala Arg Arg Ser Asn Glu Glu 
            100                 105                 110         


Arg Gly Lys Leu Val Thr Arg Ile Gln Thr Ala Val Lys Ser Val Ala 
        115                 120                 125             


Asn Ser Gln Asp Ile Asp Leu Val Val Asp Ala Asn Ala Val Ala Tyr 
    130                 135                 140                 


Asn Ser Ser Asp Val Lys Asp Ile Thr Ala Asp Val Leu Lys Gln Val 
145                 150                 155                 160 


Lys 
    


