               SEQUENCE LISTING

<110> DSM IP Assets B.V.

<120> PROCESS FOR ENZYMATIC OIL DEGUMMING

<130> 32910-WO-PCT

<150> EP18171015.3 
<151> 2018-05-07

<160> 4

<170> BiSSAP 1.3.6

<210> 1
<211> 298
<212> PRT
<213> Aspergillus niger


<220> 
<223> Amino acid sequence of phospholipase A1

<400> 1
Met Phe Leu Arg Arg Glu Phe Gly Ala Val Ala Ala Leu Ser Val Leu 
1               5                   10                  15      
Ala His Ala Ala Pro Ala Pro Ala Pro Met Gln Arg Arg Asp Ile Ser 
            20                  25                  30          
Ser Thr Val Leu Asp Asn Ile Asp Leu Phe Ala Gln Tyr Ser Ala Ala 
        35                  40                  45              
Ala Tyr Cys Ser Ser Asn Ile Glu Ser Thr Gly Thr Thr Leu Thr Cys 
    50                  55                  60                  
Asp Val Gly Asn Cys Pro Leu Val Glu Ala Ala Gly Ala Thr Thr Ile 
65                  70                  75                  80  
Asp Glu Phe Asp Asp Ser Ser Ser Tyr Gly Asp Pro Thr Gly Phe Ile 
                85                  90                  95      
Ala Val Asp Pro Thr Asn Glu Leu Ile Val Leu Ser Phe Arg Gly Ser 
            100                 105                 110         
Ser Asp Leu Ser Asn Trp Ile Ala Asp Leu Asp Phe Gly Leu Thr Ser 
        115                 120                 125             
Val Ser Ser Ile Cys Asp Gly Cys Glu Met His Lys Gly Phe Tyr Glu 
    130                 135                 140                 
Ala Trp Glu Val Ile Ala Asp Thr Ile Thr Ser Lys Val Glu Ala Ala 
145                 150                 155                 160 
Val Ser Ser Tyr Pro Asp Tyr Thr Leu Val Phe Thr Gly His Ser Tyr 
                165                 170                 175     
Gly Ala Ala Leu Ala Ala Val Ala Ala Thr Val Leu Arg Asn Ala Gly 
            180                 185                 190         
Tyr Thr Leu Asp Leu Tyr Asn Phe Gly Gln Pro Arg Ile Gly Asn Leu 
        195                 200                 205             
Ala Leu Ala Asp Tyr Ile Thr Asp Gln Asn Met Gly Ser Asn Tyr Arg 
    210                 215                 220                 
Val Thr His Thr Asp Asp Ile Val Pro Lys Leu Pro Pro Glu Leu Leu 
225                 230                 235                 240 
Gly Tyr His His Phe Ser Pro Glu Tyr Trp Ile Thr Ser Gly Asn Asp 
                245                 250                 255     
Val Thr Val Thr Thr Ser Asp Val Thr Glu Val Val Gly Val Asp Ser 
            260                 265                 270         
Thr Ala Gly Asn Asp Gly Thr Leu Leu Asp Ser Thr Thr Ala His Arg 
        275                 280                 285             
Trp Tyr Thr Ile Tyr Ile Ser Glu Cys Ser 
    290                 295             

<210> 2
<211> 897
<212> DNA
<213> Aspergillus niger


<220> 
<223> DNA sequence of phospholipase A1 (Genbank: XM_001393495)

<400> 2
atgtttctcc gcagggaatt tggggctgtt gcagccctat ctgtgctggc ccatgctgct      60

cccgcacctg ctccgatgca gcgtagagac atctcctcta ccgtcttgga caatatcgac     120

ctcttcgccc aatacagtgc agcagcttac tgctcctcca acatcgagtc caccggcacg     180

actctgacct gcgacgtagg caattgccct ctcgtcgagg cagccggtgc cacgaccatc     240

gatgagtttg acgacagcag cagctacggc gacccgacgg ggttcatcgc cgttgacccg     300

acgaacgagt taatcgttct gtctttccgg ggcagttccg acctctcgaa ctggattgcc     360

gacctagact tcggcctcac atccgtaagc agcatctgtg atggctgtga gatgcacaag     420

ggcttctacg aggcctggga agtcattgcc gacaccatca catccaaggt ggaggccgcc     480

gtctccagct atccggacta caccctcgtg ttcaccggac acagctacgg cgctgcattg     540

gcggctgtcg cggccaccgt gctccgcaac gccggataca ctcttgacct gtacaacttc     600

ggccagcccc gtattggcaa cctcgcctta gccgactaca tcaccgacca aaacatgggc     660

agcaactacc gcgtcacgca caccgatgac atcgtgccta agctgcctcc ggagctgctg     720

ggctaccacc acttcagtcc ggagtactgg atcaccagcg gcaatgatgt gacggtgaca     780

acgtcggacg tcaccgaggt cgtgggggtg gattcgacgg ctgggaatga cggcacgctg     840

cttgacagta cgactgccca tcggtggtac acgatctaca ttagtgaatg ctcgtag        897


<210> 3
<211> 245
<212> PRT
<213> Artificial Sequence


<220> 
<223> Phospholipase C from WO2005/086900

<400> 3
Trp Ser Ala Glu Asp Lys His Asn Glu Gly Ile Asn Ser His Leu Trp 
1               5                   10                  15      
Ile Val Asn Arg Ala Ile Asp Ile Met Ser Arg Asn Thr Thr Ile Val 
            20                  25                  30          
Asn Pro Asn Glu Thr Ala Leu Leu Asn Glu Trp Arg Ala Asp Leu Glu 
        35                  40                  45              
Asn Gly Ile Tyr Ser Ala Asp Tyr Glu Asn Pro Tyr Tyr Asp Asp Ser 
    50                  55                  60                  
Thr Tyr Ala Ser His Phe Tyr Asp Pro Asp Thr Gly Thr Thr Tyr Ile 
65                  70                  75                  80  
Pro Phe Ala Lys His Ala Lys Glu Thr Gly Ala Lys Tyr Phe Asn Leu 
                85                  90                  95      
Ala Gly Gln Ala Tyr Gln Asn Gln Asp Met Gln Gln Ala Phe Phe Tyr 
            100                 105                 110         
Leu Gly Leu Ser Leu His Tyr Leu Gly Asp Val Asn Gln Pro Met His 
        115                 120                 125             
Ala Ala Ser Phe Thr Asp Leu Ser Tyr Pro Met Gly Phe His Ser Lys 
    130                 135                 140                 
Tyr Glu Asn Phe Val Asp Thr Ile Lys Asn Asn Tyr Ile Val Ser Asp 
145                 150                 155                 160 
Ser Asn Gly Tyr Trp Asn Trp Lys Gly Ala Asn Pro Glu Asp Trp Ile 
                165                 170                 175     
Glu Gly Ala Ala Val Ala Ala Lys Gln Asp Tyr Pro Gly Val Val Asn 
            180                 185                 190         
Asp Thr Thr Lys Asp Trp Phe Val Lys Ala Ala Val Ser Gln Glu Tyr 
        195                 200                 205             
Ala Asp Lys Trp Arg Ala Glu Val Thr Pro Val Thr Gly Lys Arg Leu 
    210                 215                 220                 
Met Glu Ala Gln Arg Val Thr Ala Gly Tyr Ile His Leu Trp Phe Asp 
225                 230                 235                 240 
Thr Tyr Val Asn Arg 
                245 

<210> 4
<211> 298
<212> PRT
<213> Artificial Sequence


<220> 
<223> Phosphatidylinositol phospholipase C from WO2011/046812

<400> 4
Met Ala Ser Ser Ile Asn Val Leu Glu Asn Trp Ser Arg Trp Met Lys 
1               5                   10                  15      
Pro Ile Asn Asp Asp Ile Pro Leu Ala Arg Ile Ser Ile Pro Gly Thr 
            20                  25                  30          
His Asp Ser Gly Thr Phe Lys Leu Gln Asn Pro Ile Lys Gln Val Trp 
        35                  40                  45              
Gly Met Thr Gln Glu Tyr Asp Phe Arg Tyr Gln Met Asp His Gly Ala 
    50                  55                  60                  
Arg Ile Phe Asp Ile Arg Gly Arg Leu Thr Asp Asp Asn Thr Ile Val 
65                  70                  75                  80  
Leu His His Gly Pro Leu Tyr Leu Tyr Val Thr Leu His Glu Phe Ile 
                85                  90                  95      
Asn Glu Ala Lys Gln Phe Leu Lys Asp Asn Pro Ser Glu Thr Ile Ile 
            100                 105                 110         
Met Ser Leu Lys Lys Glu Tyr Glu Asp Met Lys Gly Ala Glu Ser Ser 
        115                 120                 125             
Phe Ser Ser Thr Phe Glu Lys Asn Tyr Phe Arg Asp Pro Ile Phe Leu 
    130                 135                 140                 
Lys Thr Glu Gly Asn Ile Lys Leu Gly Asp Ala Arg Gly Lys Ile Val 
145                 150                 155                 160 
Leu Leu Lys Arg Tyr Ser Gly Ser Asn Glu Ser Gly Gly Tyr Asn Phe 
                165                 170                 175     
Phe Tyr Trp Pro Asp Asn Glu Thr Phe Thr Ser Thr Ile Asn Gly Asn 
            180                 185                 190         
Val Asn Val Thr Val Gln Asp Lys Tyr Lys Val Ser Leu Asp Glu Lys 
        195                 200                 205             
Ile Asn Ala Ile Lys Asp Thr Leu Asn Glu Thr Ile Asn Asn Ser Glu 
    210                 215                 220                 
Asp Val Asn His Leu Tyr Ile Asn Phe Thr Ser Leu Ser Ser Gly Gly 
225                 230                 235                 240 
Thr Ala Trp Thr Ser Pro Tyr Tyr Tyr Ala Ser Arg Ile Asn Pro Glu 
                245                 250                 255     
Ile Ala Asn Tyr Ile Lys Gln Lys Asn Pro Thr Arg Val Gly Trp Ile 
            260                 265                 270         
Ile Gln Asp Phe Ile Asn Glu Lys Trp His Pro Leu Leu Tyr Gln Glu 
        275                 280                 285             
Val Ile Asn Ala Asn Lys Ser Leu Val Lys 
    290                 295             

