﻿                         序列表

<110>  华南理工大学

<120>  一种偏甘油酯脂肪酶突变体及其应用

<160>  17

<170>  SIPOSequenceListing 1.0

<210>  1
<211>  840
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  1
gatgttagta ctagtgagct tgatcagttt gaattctggg tccaatatgc tgcagcttcc        60
tattatgaag cagactatac tgcccaagtc ggagacaaac tgtcctgttc taaaggcaac       120
tgcccagagg tagaagctac tggagctaca gtttcctacg attttagtga ctccactatt       180
accgacacgg ccggttacat agctgtcgat catacgaatt cagccgttgt gcttgctttc       240
agaggttctt attccgttag aaattgggtg gctgatgcta ctttcgttca tacaaacccc       300
ggtttatgtg atggttgtct agctgagctg ggtttctggt cttcatggaa gttggtgcga       360
gacgacatca tcaaggaatt gaaggaggtt gttgcccaaa atcctaacta cgaactagtt       420
gtagttggac attctttggg tgctgccgtt gccacattgg cagctactga cttaagagga       480
aaaggatacc cttcagctaa gttgtacgct tatgcctctc ctagggtagg taatgccgca       540
ttggccaagt acattacagc tcagggtaac aacttcagat ttacacacac caatgaccca       600
gtgcccaaac tgccattgtt gtcaatgggg tacgttcacg tttctcctga atactggatc       660
acctccccta acaatgcaac cgtctctacc agtgatataa aagtcattga tggagatgta       720
tctttcgatg gaaacactgg tactgggtta ccactgttga ctgattttga ggcacacatt       780
tggtattttg ttcaagtgga tgcaggtaag ggcccaggcc ttccatttaa acgtgtctaa       840

<210>  2
<211>  840
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  2
gatgttagta ctagtgagct tgatcagttt gaattctggg tccaatatgc tgcagcttcc        60
tattatgaag cagactatac tgcccaagtc ggagacaaac tgtcctgttc taaaggcaac       120
tgcccagagg tagaagctac tggagctaca gtttcctacg attttagtga ctccactatt       180
accgacacgg ccggttacat agctgtcgat catacgaatt cagccgttgt gcttgctttc       240
agaggttctc gttccgttag aaattgggtg gctgatgcta ctttcgttca tacaaacccc       300
ggtttatgtg atggttgtct agctgagctg ggtttctggt cttcatggaa gttggtgcga       360
gacgacatca tcaaggaatt gaaggaggtt gttgcccaaa atcctaacta cgaactagtt       420
gtagttggac attctttggg tgctgccgtt gccacattgg cagctactga cttaagagga       480
aaaggatacc cttcagctaa gttgtacgct tatgcctctc ctagggtagg taatgccgca       540
ttggccaagt acattacagc tcagggtaac aacttcagat ttacacacac caatgaccca       600
gtgcccaaac tgccattgtt gtcaatgggg tacgttcacg tttctcctga atactggatc       660
acctccccta acaatgcaac cgtctctacc agtgatataa aagtcattga tggagatgta       720
tctttcgatg gaaacactgg tactgggtta ccactgttga ctgattttga ggcacacatt       780
tggtattttg ttcaagtgga tgcaggtaag ggcccaggcc ttccatttaa acgtgtctaa       840

<210>  3
<211>  840
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  3
gatgttagta ctagtgagct tgatcagttt gaattctggg tccaatatgc tgcagcttcc        60
tattatgaag cagactatac tgcccaagtc ggagacaaac tgtcctgttc taaaggcaac       120
tgcccagagg tagaagctac tggagctaca gtttcctacg attttagtga ctccactatt       180
accgacacgg ccggttacat agctgtcgat catacgaatt cagccgttgt gcttgctttc       240
agaggttctt attccgttag aaattgggtg gctgatgcta ctttcgttca tacaaacccc       300
ggtttatgtg atggttgtct agctgagctg ggtttctggt cttcatggaa gttggtgcga       360
gacgacatca tcaaggaatt gaaggaggtt gttgcccaaa atcctaacta cgaactagtt       420
gtagttggac attctttggg tgctgccgtt gccacattgg cagctactga cttaagagga       480
aaaggatacc cttcagctaa gttgtacgct tatgcctctc ctagggtagg taatgccgca       540
ttggccaagt acattacagc tcagggtaac aacttcagat ttacacacac caatgaccca       600
gtgcccaaac tgccattgtt gtcagcgggg tacgttcacg tttctcctga atactggatc       660
acctccccta acaatgcaac cgtctctacc agtgatataa aagtcattga tggagatgta       720
tctttcgatg gaaacactgg tactgggtta ccactgttga ctgattttga ggcacacatt       780
tggtattttg ttcaagtgga tgcaggtaag ggcccaggcc ttccatttaa acgtgtctaa       840

<210>  4
<211>  840
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  4
gatgttagta ctagtgagct tgatcagttt gaattctggg tccaatatgc tgcagcttcc        60
tattatgaag cagactatac tgcccaagtc ggagacaaac tgtcctgttc taaaggcaac       120
tgcccagagg tagaagctac tggagctaca gtttcctacg attttagtga ctccactatt       180
accgacacgg ccggttacat agctgtcgat catacgaatt cagccgttgt gcttgctttc       240
agaggttctt attccgttag aaattgggtg gctgatgcta ctttcgttca tacaaacccc       300
ggtttatgtg atggttgtct agctgagctg ggtttctggt cttcatggaa gttggtgcga       360
gacgacatca tcaaggaatt gaaggaggtt gttgcccaaa atcctaacta cgaactagtt       420
gtagttggac attctttggg tgctgccgtt gccacattgg cagctactga cttaagagga       480
aaaggatacc cttcagctaa gttgtacgct tatgcctctc ctagggtagg taatgccgca       540
ttggccaagt acattacagc tcagggtaac aacttcagat ttacacacac caatgaccca       600
gtgcccaaac tgccattgtt gtcaatgggg tacgttcacg tttctcctga atactggatc       660
acctccccta acaatgcaac cgtctctacc agtgatataa aagtcattga tggagatgta       720
tctttcgatg gaaacactgg tactgggtta ccactgttga ctgattttga ggcacacgag       780
tggtattttg ttcaagtgga tgcaggtaag ggcccaggcc ttccatttaa acgtgtctaa       840

<210>  5
<211>  840
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  5
gatgttagta ctagtgagct tgatcagttt gaattctggg tccaatatgc tgcagcttcc        60
tattatgaag cagactatac tgcccaagtc ggagacaaac tgtcctgttc taaaggcaac       120
tgcccagagg tagaagctac tggagctaca gtttcctacg attttagtga ctccactatt       180
accgacacgg ccggttacat agctgtcgat catacgaatt cagccgttgt gcttgctttc       240
agaggttctt attccgttag aaattgggtg gctgatgcta ctttcgttca tacaaacccc       300
ggtttatgtg atggttgtct agctgagctg ggtttctggt cttcatggaa gttggtgcga       360
gacgacatca tcaaggaatt gaaggaggtt gttgcccaaa atcctaacta cgaactagtt       420
gtagttggac attctttggg tgctgccgtt gccacattgg cagctactga cttaagagga       480
aaaggatacc cttcagctaa gttgtacgct tatgcctctc ctagggtagg taatgccgca       540
ttggccaagt acattacagc tcagggtaac aacttcagat ttacacacac caatgaccca       600
gtgcccaaac tgccattgtt gtcaatgggg tacgttcacg tttctcctga atactggatc       660
acctccccta acaatgcaac cgtctctacc agtgatataa aagtcattga tggagatgta       720
tctttcgatg gaaacactgg tactgggtta ccactgttga ctgattttga ggcacacagg       780
tggtattttg ttcaagtgga tgcaggtaag ggcccaggcc ttccatttaa acgtgtctaa       840

<210>  6
<211>  279
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  6
Asp Val Ser Thr Ser Glu Leu Asp Gln Phe Glu Phe Trp Val Gln Tyr 
1               5                   10                  15      
Ala Ala Ala Ser Tyr Tyr Glu Ala Asp Tyr Thr Ala Gln Val Gly Asp 
            20                  25                  30          
Lys Leu Ser Cys Ser Lys Gly Asn Cys Pro Glu Val Glu Ala Thr Gly 
        35                  40                  45              
Ala Thr Val Ser Tyr Asp Phe Ser Asp Ser Thr Ile Thr Asp Thr Ala 
    50                  55                  60                  
Gly Tyr Ile Ala Val Asp His Thr Asn Ser Ala Val Val Leu Ala Phe 
65                  70                  75                  80  
Arg Gly Ser Arg Ser Val Arg Asn Trp Val Ala Asp Ala Thr Phe Val 
                85                  90                  95      
His Thr Asn Pro Gly Leu Cys Asp Gly Cys Leu Ala Glu Leu Gly Phe 
            100                 105                 110         
Trp Ser Ser Trp Lys Leu Val Arg Asp Asp Ile Ile Lys Glu Leu Lys 
        115                 120                 125             
Glu Val Val Ala Gln Asn Pro Asn Tyr Glu Leu Val Val Val Gly His 
    130                 135                 140                 
Ser Leu Gly Ala Ala Val Ala Thr Leu Ala Ala Thr Asp Leu Arg Gly 
145                 150                 155                 160 
Lys Gly Tyr Pro Ser Ala Lys Leu Tyr Ala Tyr Ala Ser Pro Arg Val 
                165                 170                 175     
Gly Asn Ala Ala Leu Ala Lys Tyr Ile Thr Ala Gln Gly Asn Asn Phe 
            180                 185                 190         
Arg Phe Thr His Thr Asn Asp Pro Val Pro Lys Leu Pro Leu Leu Ser 
        195                 200                 205             
Met Gly Tyr Val His Val Ser Pro Glu Tyr Trp Ile Thr Ser Pro Asn 
    210                 215                 220                 
Asn Ala Thr Val Ser Thr Ser Asp Ile Lys Val Ile Asp Gly Asp Val 
225                 230                 235                 240 
Ser Phe Asp Gly Asn Thr Gly Thr Gly Leu Pro Leu Leu Thr Asp Phe 
                245                 250                 255     
Glu Ala His Ile Trp Tyr Phe Val Gln Val Asp Ala Gly Lys Gly Pro 
            260                 265                 270         
Gly Leu Pro Phe Lys Arg Val 
        275                 

<210>  7
<211>  279
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  7
Asp Val Ser Thr Ser Glu Leu Asp Gln Phe Glu Phe Trp Val Gln Tyr 
1               5                   10                  15      
Ala Ala Ala Ser Tyr Tyr Glu Ala Asp Tyr Thr Ala Gln Val Gly Asp 
            20                  25                  30          
Lys Leu Ser Cys Ser Lys Gly Asn Cys Pro Glu Val Glu Ala Thr Gly 
        35                  40                  45              
Ala Thr Val Ser Tyr Asp Phe Ser Asp Ser Thr Ile Thr Asp Thr Ala 
    50                  55                  60                  
Gly Tyr Ile Ala Val Asp His Thr Asn Ser Ala Val Val Leu Ala Phe 
65                  70                  75                  80  
Arg Gly Ser Tyr Ser Val Arg Asn Trp Val Ala Asp Ala Thr Phe Val 
                85                  90                  95      
His Thr Asn Pro Gly Leu Cys Asp Gly Cys Leu Ala Glu Leu Gly Phe 
            100                 105                 110         
Trp Ser Ser Trp Lys Leu Val Arg Asp Asp Ile Ile Lys Glu Leu Lys 
        115                 120                 125             
Glu Val Val Ala Gln Asn Pro Asn Tyr Glu Leu Val Val Val Gly His 
    130                 135                 140                 
Ser Leu Gly Ala Ala Val Ala Thr Leu Ala Ala Thr Asp Leu Arg Gly 
145                 150                 155                 160 
Lys Gly Tyr Pro Ser Ala Lys Leu Tyr Ala Tyr Ala Ser Pro Arg Val 
                165                 170                 175     
Gly Asn Ala Ala Leu Ala Lys Tyr Ile Thr Ala Gln Gly Asn Asn Phe 
            180                 185                 190         
Arg Phe Thr His Thr Asn Asp Pro Val Pro Lys Leu Pro Leu Leu Ser 
        195                 200                 205             
Ala Gly Tyr Val His Val Ser Pro Glu Tyr Trp Ile Thr Ser Pro Asn 
    210                 215                 220                 
Asn Ala Thr Val Ser Thr Ser Asp Ile Lys Val Ile Asp Gly Asp Val 
225                 230                 235                 240 
Ser Phe Asp Gly Asn Thr Gly Thr Gly Leu Pro Leu Leu Thr Asp Phe 
                245                 250                 255     
Glu Ala His Ile Trp Tyr Phe Val Gln Val Asp Ala Gly Lys Gly Pro 
            260                 265                 270         
Gly Leu Pro Phe Lys Arg Val 
        275                 

<210>  8
<211>  279
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  8
Asp Val Ser Thr Ser Glu Leu Asp Gln Phe Glu Phe Trp Val Gln Tyr 
1               5                   10                  15      
Ala Ala Ala Ser Tyr Tyr Glu Ala Asp Tyr Thr Ala Gln Val Gly Asp 
            20                  25                  30          
Lys Leu Ser Cys Ser Lys Gly Asn Cys Pro Glu Val Glu Ala Thr Gly 
        35                  40                  45              
Ala Thr Val Ser Tyr Asp Phe Ser Asp Ser Thr Ile Thr Asp Thr Ala 
    50                  55                  60                  
Gly Tyr Ile Ala Val Asp His Thr Asn Ser Ala Val Val Leu Ala Phe 
65                  70                  75                  80  
Arg Gly Ser Tyr Ser Val Arg Asn Trp Val Ala Asp Ala Thr Phe Val 
                85                  90                  95      
His Thr Asn Pro Gly Leu Cys Asp Gly Cys Leu Ala Glu Leu Gly Phe 
            100                 105                 110         
Trp Ser Ser Trp Lys Leu Val Arg Asp Asp Ile Ile Lys Glu Leu Lys 
        115                 120                 125             
Glu Val Val Ala Gln Asn Pro Asn Tyr Glu Leu Val Val Val Gly His 
    130                 135                 140                 
Ser Leu Gly Ala Ala Val Ala Thr Leu Ala Ala Thr Asp Leu Arg Gly 
145                 150                 155                 160 
Lys Gly Tyr Pro Ser Ala Lys Leu Tyr Ala Tyr Ala Ser Pro Arg Val 
                165                 170                 175     
Gly Asn Ala Ala Leu Ala Lys Tyr Ile Thr Ala Gln Gly Asn Asn Phe 
            180                 185                 190         
Arg Phe Thr His Thr Asn Asp Pro Val Pro Lys Leu Pro Leu Leu Ser 
        195                 200                 205             
Met Gly Tyr Val His Val Ser Pro Glu Tyr Trp Ile Thr Ser Pro Asn 
    210                 215                 220                 
Asn Ala Thr Val Ser Thr Ser Asp Ile Lys Val Ile Asp Gly Asp Val 
225                 230                 235                 240 
Ser Phe Asp Gly Asn Thr Gly Thr Gly Leu Pro Leu Leu Thr Asp Phe 
                245                 250                 255     
Glu Ala His Glu Trp Tyr Phe Val Gln Val Asp Ala Gly Lys Gly Pro 
            260                 265                 270         
Gly Leu Pro Phe Lys Arg Val 
        275                 

<210>  9
<211>  279
<212>  PRT
<213>  人工序列(Artificial Sequence)

<400>  9
Asp Val Ser Thr Ser Glu Leu Asp Gln Phe Glu Phe Trp Val Gln Tyr 
1               5                   10                  15      
Ala Ala Ala Ser Tyr Tyr Glu Ala Asp Tyr Thr Ala Gln Val Gly Asp 
            20                  25                  30          
Lys Leu Ser Cys Ser Lys Gly Asn Cys Pro Glu Val Glu Ala Thr Gly 
        35                  40                  45              
Ala Thr Val Ser Tyr Asp Phe Ser Asp Ser Thr Ile Thr Asp Thr Ala 
    50                  55                  60                  
Gly Tyr Ile Ala Val Asp His Thr Asn Ser Ala Val Val Leu Ala Phe 
65                  70                  75                  80  
Arg Gly Ser Tyr Ser Val Arg Asn Trp Val Ala Asp Ala Thr Phe Val 
                85                  90                  95      
His Thr Asn Pro Gly Leu Cys Asp Gly Cys Leu Ala Glu Leu Gly Phe 
            100                 105                 110         
Trp Ser Ser Trp Lys Leu Val Arg Asp Asp Ile Ile Lys Glu Leu Lys 
        115                 120                 125             
Glu Val Val Ala Gln Asn Pro Asn Tyr Glu Leu Val Val Val Gly His 
    130                 135                 140                 
Ser Leu Gly Ala Ala Val Ala Thr Leu Ala Ala Thr Asp Leu Arg Gly 
145                 150                 155                 160 
Lys Gly Tyr Pro Ser Ala Lys Leu Tyr Ala Tyr Ala Ser Pro Arg Val 
                165                 170                 175     
Gly Asn Ala Ala Leu Ala Lys Tyr Ile Thr Ala Gln Gly Asn Asn Phe 
            180                 185                 190         
Arg Phe Thr His Thr Asn Asp Pro Val Pro Lys Leu Pro Leu Leu Ser 
        195                 200                 205             
Met Gly Tyr Val His Val Ser Pro Glu Tyr Trp Ile Thr Ser Pro Asn 
    210                 215                 220                 
Asn Ala Thr Val Ser Thr Ser Asp Ile Lys Val Ile Asp Gly Asp Val 
225                 230                 235                 240 
Ser Phe Asp Gly Asn Thr Gly Thr Gly Leu Pro Leu Leu Thr Asp Phe 
                245                 250                 255     
Glu Ala His Arg Trp Tyr Phe Val Gln Val Asp Ala Gly Lys Gly Pro 
            260                 265                 270         
Gly Leu Pro Phe Lys Arg Val 
        275                 

<210>  10
<211>  37
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  10
cttgctttca gaggttctcg ttccgttaga aattggg                                 37

<210>  11
<211>  37
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  11
cccaatttct aacggaacga gaacctctga aagcaag                                 37

<210>  12
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  12
gtgaacgtac cccgctgaca acaatggcag tttgggcact                              40

<210>  13
<211>  40
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  13
agtgcccaaa ctgccattgt tgtcagcggg gtacgttcac                              40

<210>  14
<211>  41
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  14
gactgatttt gaggcacacg agtggtattt tgttcaagtg g                            41

<210>  15
<211>  41
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  15
ccacttgaac aaaataccac tcgtgtgcct caaaatcagt c                            41

<210>  16
<211>  38
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  16
ctgattttga ggcacacagg tggtattttg ttcaagtg                                38

<210>  17
<211>  38
<212>  DNA
<213>  人工序列(Artificial Sequence)

<400>  17
cacttgaaca aaataccacc tgtgtgcctc aaaatcag                                38

