                         SEQUENCE LISTING

<110>  BioVersys AG
 
<120>  BACTERIAL VECTORS FOR GENETIC MANIPULATION OF BACTERIA

<130>  P5410PC00

<150>  EP18172731.4
<151>  2018-05-16

<160>  22    

<170>  PatentIn version 3.5

<210>  1
<211>  654
<212>  DNA
<213>  Acinetobacter baylyi

<400>  1
atgcaacatg agttctggca tcaacgctgg caggaaaaca gaatcggttt tcatcaattt       60

acaccgagtc cactgttagt cgattatttt aacgaacttg gcttaaaaac ctctgcccgt      120

atctttgttc cattgagtgg taaaacactg gatattagtt ggttattaca acaaggctac      180

catgttgtgg caattgagct tagccaaatc gctgtaacat cattgattga gcaattagtt      240

gaagacttcg atatacaatt tgagtcttcc gaaaagaata atctgatcca ttaccatcac      300

ccacaaattg atatttttgt cggtgatttt tttgatctaa gcaaagaaca attaggacag      360

gtcgatgcaa tttttgatcg tgcagcattg attgctttac cagacgacat acgccaagac      420

tatgttcaac acttgatcga gatcagtggt gctgcatctc agtttttaat tagctaccaa      480

tacgatgcag gcagtcatga aggacctcct ttttcggtca atgcagaaga aattaaacaa      540

ctttatgctg aggcttatga catccgactt ttgaaggagc aacttgtcga tgcatctcaa      600

aacaaaggaa atcaccccaa gagcacacta tggatactca ctgccaagtt ctga            654


<210>  2
<211>  217
<212>  PRT
<213>  Acinetobacter baylyi

<400>  2

Met Gln His Glu Phe Trp His Gln Arg Trp Gln Glu Asn Arg Ile Gly 
1               5                   10                  15      


Phe His Gln Phe Thr Pro Ser Pro Leu Leu Val Asp Tyr Phe Asn Glu 
            20                  25                  30          


Leu Gly Leu Lys Thr Ser Ala Arg Ile Phe Val Pro Leu Ser Gly Lys 
        35                  40                  45              


Thr Leu Asp Ile Ser Trp Leu Leu Gln Gln Gly Tyr His Val Val Ala 
    50                  55                  60                  


Ile Glu Leu Ser Gln Ile Ala Val Thr Ser Leu Ile Glu Gln Leu Val 
65                  70                  75                  80  


Glu Asp Phe Asp Ile Gln Phe Glu Ser Ser Glu Lys Asn Asn Leu Ile 
                85                  90                  95      


His Tyr His His Pro Gln Ile Asp Ile Phe Val Gly Asp Phe Phe Asp 
            100                 105                 110         


Leu Ser Lys Glu Gln Leu Gly Gln Val Asp Ala Ile Phe Asp Arg Ala 
        115                 120                 125             


Ala Leu Ile Ala Leu Pro Asp Asp Ile Arg Gln Asp Tyr Val Gln His 
    130                 135                 140                 


Leu Ile Glu Ile Ser Gly Ala Ala Ser Gln Phe Leu Ile Ser Tyr Gln 
145                 150                 155                 160 


Tyr Asp Ala Gly Ser His Glu Gly Pro Pro Phe Ser Val Asn Ala Glu 
                165                 170                 175     


Glu Ile Lys Gln Leu Tyr Ala Glu Ala Tyr Asp Ile Arg Leu Leu Lys 
            180                 185                 190         


Glu Gln Leu Val Asp Ala Ser Gln Asn Lys Gly Asn His Pro Lys Ser 
        195                 200                 205             


Thr Leu Trp Ile Leu Thr Ala Lys Phe 
    210                 215         


<210>  3
<211>  702
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  I-SceI vector

<400>  3
atgcatcaaa aaaaccaggt aatgaacctg ggtccgaact ctaaactgct gaaagaatac       60

aaatcccagc tgatcgaact gaacatcgaa cagttcgaag caggtatcgg tctgatcctg      120

ggtgatgctt acatccgttc tcgtgatgaa ggtaaaacct actgtatgca gttcgagtgg      180

aaaaacaaag catacatgga ccacgtatgt ctgctgtacg atcagtgggt actgtccccg      240

ccgcacaaaa aacaacgtgt taaccacctg ggtaacctgg taatcacctg gggcgcccag      300

actttcaaac accaagcttt caacaaactg gctaacctgt tcatcgttaa caacaaaaaa      360

accatcccga acaacctggt tgaaaactac ctgaccccga tgtctctggc atactggttc      420

atggatgatg gtggtaaatg ggattacaac aaaaactcta ccaacaaatc gatcgtactg      480

aacacccagt ctttcacttt cgaagaagta gaatacctgg ttaagggtct gcgtaacaaa      540

ttccaactga actgttacgt aaaaatcaac aaaaacaaac cgatcatcta catcgattct      600

atgtcttacc tgatcttcta caacctgatc aaaccgtacc tgatcccgca gatgatgtac      660

aaactgccga acactatctc ctccgaaact ttcctgaaat aa                         702


<210>  4
<211>  233
<212>  PRT
<213>  Artificial Sequence

<220>
<223>  I-SceI protein

<400>  4

Met His Gln Lys Asn Gln Val Met Asn Leu Gly Pro Asn Ser Lys Leu 
1               5                   10                  15      


Leu Lys Glu Tyr Lys Ser Gln Leu Ile Glu Leu Asn Ile Glu Gln Phe 
            20                  25                  30          


Glu Ala Gly Ile Gly Leu Ile Leu Gly Asp Ala Tyr Ile Arg Ser Arg 
        35                  40                  45              


Asp Glu Gly Lys Thr Tyr Cys Met Gln Phe Glu Trp Lys Asn Lys Ala 
    50                  55                  60                  


Tyr Met Asp His Val Cys Leu Leu Tyr Asp Gln Trp Val Leu Ser Pro 
65                  70                  75                  80  


Pro His Lys Lys Gln Arg Val Asn His Leu Gly Asn Leu Val Ile Thr 
                85                  90                  95      


Trp Gly Ala Gln Thr Phe Lys His Gln Ala Phe Asn Lys Leu Ala Asn 
            100                 105                 110         


Leu Phe Ile Val Asn Asn Lys Lys Thr Ile Pro Asn Asn Leu Val Glu 
        115                 120                 125             


Asn Tyr Leu Thr Pro Met Ser Leu Ala Tyr Trp Phe Met Asp Asp Gly 
    130                 135                 140                 


Gly Lys Trp Asp Tyr Asn Lys Asn Ser Thr Asn Lys Ser Ile Val Leu 
145                 150                 155                 160 


Asn Thr Gln Ser Phe Thr Phe Glu Glu Val Glu Tyr Leu Val Lys Gly 
                165                 170                 175     


Leu Arg Asn Lys Phe Gln Leu Asn Cys Tyr Val Lys Ile Asn Lys Asn 
            180                 185                 190         


Lys Pro Ile Ile Tyr Ile Asp Ser Met Ser Tyr Leu Ile Phe Tyr Asn 
        195                 200                 205             


Leu Ile Lys Pro Tyr Leu Ile Pro Gln Met Met Tyr Lys Leu Pro Asn 
    210                 215                 220                 


Thr Ile Ser Ser Glu Thr Phe Leu Lys 
225                 230             


<210>  5
<211>  618
<212>  DNA
<213>  Escherichia coli

<400>  5
atggcacagc tatatttcta ctattccgca atgaatgcgg gtaagtctac agcattgttg       60

caatcttcat acaattacca ggaacgcggc atgcgcactg tcgtatatac ggcagaaatt      120

gatgatcgct ttggtgccgg gaaagtcagt tcgcgtatag gtttgtcatc gcctgcaaaa      180

ttatttaacc aaaattcatc attatttgat gagattcgtg cggaacatga acagcaggca      240

attcattgcg tactggttga tgaatgccag tttttaacca gacaacaagt atatgaatta      300

tcggaggttg tcgatcaact cgatataccc gtactttgtt atggtttacg taccgatttt      360

cgaggtgaat tatttattgg cagccaatac ttactggcat ggtccgacaa actggttgaa      420

ttaaaaacca tctgtttttg tggccgtaaa gcaagcatgg tgctgcgtct tgatcaagca      480

ggcagacctt ataacgaagg tgagcaggtg gtaattggtg gtaatgaacg atacgtttct      540

gtatgccgta aacactataa agaggcgtta caagtcgact cattaacggc tattcaggaa      600

aggcatcgcc acgattaa                                                    618


<210>  6
<211>  205
<212>  PRT
<213>  Escherichia coli

<400>  6

Met Ala Gln Leu Tyr Phe Tyr Tyr Ser Ala Met Asn Ala Gly Lys Ser 
1               5                   10                  15      


Thr Ala Leu Leu Gln Ser Ser Tyr Asn Tyr Gln Glu Arg Gly Met Arg 
            20                  25                  30          


Thr Val Val Tyr Thr Ala Glu Ile Asp Asp Arg Phe Gly Ala Gly Lys 
        35                  40                  45              


Val Ser Ser Arg Ile Gly Leu Ser Ser Pro Ala Lys Leu Phe Asn Gln 
    50                  55                  60                  


Asn Ser Ser Leu Phe Asp Glu Ile Arg Ala Glu His Glu Gln Gln Ala 
65                  70                  75                  80  


Ile His Cys Val Leu Val Asp Glu Cys Gln Phe Leu Thr Arg Gln Gln 
                85                  90                  95      


Val Tyr Glu Leu Ser Glu Val Val Asp Gln Leu Asp Ile Pro Val Leu 
            100                 105                 110         


Cys Tyr Gly Leu Arg Thr Asp Phe Arg Gly Glu Leu Phe Ile Gly Ser 
        115                 120                 125             


Gln Tyr Leu Leu Ala Trp Ser Asp Lys Leu Val Glu Leu Lys Thr Ile 
    130                 135                 140                 


Cys Phe Cys Gly Arg Lys Ala Ser Met Val Leu Arg Leu Asp Gln Ala 
145                 150                 155                 160 


Gly Arg Pro Tyr Asn Glu Gly Glu Gln Val Val Ile Gly Gly Asn Glu 
                165                 170                 175     


Arg Tyr Val Ser Val Cys Arg Lys His Tyr Lys Glu Ala Leu Gln Val 
            180                 185                 190         


Asp Ser Leu Thr Ala Ile Gln Glu Arg His Arg His Asp 
        195                 200                 205 


<210>  7
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK354

<400>  7
ccgagcgttc tgaacaaatc                                                   20


<210>  8
<211>  45
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK384

<400>  8
ggtattcagt ggagatacac ccgggagttc ggaagggacg ggatg                       45


<210>  9
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK385

<400>  9
gctctagatg tcgcgggaag cgatgaag                                          28


<210>  10
<211>  21
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK389

<400>  10
atggcaacac aaggagtgtt c                                                 21


<210>  11
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK390

<400>  11
agcggccagt tgatcaccac                                                   20


<210>  12
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK391

<400>  12
gcttcctcca gtgccagttc                                                   20


<210>  13
<211>  28
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK393

<400>  13
tcccccgggt gtatctccac tgaatacc                                          28


<210>  14
<211>  34
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK395

<400>  14
aaggcctgga attcggatcg aggcggaaca atac                                   34


<210>  15
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK454

<400>  15
cgtctctcgg aaagttcttg                                                   20


<210>  16
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK455

<400>  16
tcgtaaatca tgactgaccc                                                   20


<210>  17
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK456

<400>  17
cgtctgttaa cccgtgaatc                                                   20


<210>  18
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK457

<400>  18
cgctccatcg cagagtattc                                                   20


<210>  19
<211>  44
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK612

<400>  19
gaggaattcg agctcggtac ccgtattcga tctcgttgac atac                        44


<210>  20
<211>  39
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK613

<400>  20
cgcgtttcat cccgggtgtt tgtactccca aaggttcac                              39


<210>  21
<211>  31
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK614

<400>  21
aacacccggg atgaaacgcg tgcgcttttc g                                      31


<210>  22
<211>  46
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  primer oCK615

<400>  22
ctatcaacag gagtccaaga ctagtgtcat cagccagctg cttcac                      46


