                         SEQUENCE LISTING

<110>  University of Massachusetts
 
<120>  PERFUSION-BASED DELIVERY OF RECOMBINANT AAV VECTORS FOR 
       EXPRESSION OF SECRETED PROTEINS

<130>  U0120.70100WO00

<140>  Not Yet Assigned
<141>  Concurrently herewith

<150>  62/672,531
<151>  2018-05-16

<160>  2     

<170>  PatentIn version 3.5

<210>  1
<211>  418
<212>  PRT
<213>  Human

<400>  1

Met Pro Ser Ser Val Ser Trp Gly Ile Leu Leu Leu Ala Gly Leu Cys 
1               5                   10                  15      


Cys Leu Val Pro Val Ser Leu Ala Glu Asp Pro Gln Gly Asp Ala Ala 
            20                  25                  30          


Gln Lys Thr Asp Thr Ser His His Asp Gln Asp His Pro Thr Phe Asn 
        35                  40                  45              


Lys Ile Thr Pro Asn Leu Ala Glu Phe Ala Phe Ser Leu Tyr Arg Gln 
    50                  55                  60                  


Leu Ala His Gln Ser Asn Ser Thr Asn Ile Phe Phe Ser Pro Val Ser 
65                  70                  75                  80  


Ile Ala Thr Ala Phe Ala Met Leu Ser Leu Gly Thr Lys Ala Asp Thr 
                85                  90                  95      


His Asp Glu Ile Leu Glu Gly Leu Asn Phe Asn Leu Thr Glu Ile Pro 
            100                 105                 110         


Glu Ala Gln Ile His Glu Gly Phe Gln Glu Leu Leu Arg Thr Leu Asn 
        115                 120                 125             


Gln Pro Asp Ser Gln Leu Gln Leu Thr Thr Gly Asn Gly Leu Phe Leu 
    130                 135                 140                 


Ser Glu Gly Leu Lys Leu Val Asp Lys Phe Leu Glu Asp Val Lys Lys 
145                 150                 155                 160 


Leu Tyr His Ser Glu Ala Phe Thr Val Asn Phe Gly Asp Thr Glu Glu 
                165                 170                 175     


Ala Lys Lys Gln Ile Asn Asp Tyr Val Glu Lys Gly Thr Gln Gly Lys 
            180                 185                 190         


Ile Val Asp Leu Val Lys Glu Leu Asp Arg Asp Thr Val Phe Ala Leu 
        195                 200                 205             


Val Asn Tyr Ile Phe Phe Lys Gly Lys Trp Glu Arg Pro Phe Glu Val 
    210                 215                 220                 


Lys Asp Thr Glu Glu Glu Asp Phe His Val Asp Gln Val Thr Thr Val 
225                 230                 235                 240 


Lys Val Pro Met Met Lys Arg Leu Gly Met Phe Asn Ile Gln His Cys 
                245                 250                 255     


Lys Lys Leu Ser Ser Trp Val Leu Leu Met Lys Tyr Leu Gly Asn Ala 
            260                 265                 270         


Thr Ala Ile Phe Phe Leu Pro Asp Glu Gly Lys Leu Gln His Leu Glu 
        275                 280                 285             


Asn Glu Leu Thr His Asp Ile Ile Thr Lys Phe Leu Glu Asn Glu Asp 
    290                 295                 300                 


Arg Arg Ser Ala Ser Leu His Leu Pro Lys Leu Ser Ile Thr Gly Thr 
305                 310                 315                 320 


Tyr Asp Leu Lys Ser Val Leu Gly Gln Leu Gly Ile Thr Lys Val Phe 
                325                 330                 335     


Ser Asn Gly Ala Asp Leu Ser Gly Val Thr Glu Glu Ala Pro Leu Lys 
            340                 345                 350         


Leu Ser Lys Ala Val His Lys Ala Val Leu Thr Ile Asp Glu Lys Gly 
        355                 360                 365             


Thr Glu Ala Ala Gly Ala Met Phe Leu Glu Ala Ile Pro Met Ser Ile 
    370                 375                 380                 


Pro Pro Glu Val Lys Phe Asn Lys Pro Phe Val Phe Leu Met Ile Glu 
385                 390                 395                 400 


Gln Asn Thr Lys Ser Pro Leu Phe Met Gly Lys Val Val Asn Pro Thr 
                405                 410                 415     


Gln Lys 
        


<210>  2
<211>  3300
<212>  RNA
<213>  Human

<400>  2
ugggcaggaa cugggcacug ugcccagggc augcacugcc uccacgcagc aacccucaga       60

guccugagcu gaaccaagaa ggaggagggg gucgggccuc cgaggaaggc cuagccgcug      120

cugcugccag gaauuccagg uuggaggggc ggcaaccucc ugccagccuu caggccacuc      180

uccugugccu gccagaagag acagagcuug aggagagcuu gaggagagca ggaaagccuc      240

ccccguugcc ccucuggauc cacugcuuaa auacggacga ggacagggcc cugucuccuc      300

agcuucaggc accaccacug accugggaca gugaaucgac aaugccgucu ucugucucgu      360

ggggcauccu ccugcuggca ggccugugcu gccugguccc ugucucccug gcugaggauc      420

cccagggaga ugcugcccag aagacagaua caucccacca ugaucaggau cacccaaccu      480

ucaacaagau cacccccaac cuggcugagu ucgccuucag ccuauaccgc cagcuggcac      540

accaguccaa cagcaccaau aucuucuucu ccccagugag caucgcuaca gccuuugcaa      600

ugcucucccu ggggaccaag gcugacacuc acgaugaaau ccuggagggc cugaauuuca      660

accucacgga gauuccggag gcucagaucc augaaggcuu ccaggaacuc cuccguaccc      720

ucaaccagcc agacagccag cuccagcuga ccaccggcaa uggccuguuc cucagcgagg      780

gccugaagcu aguggauaag uuuuuggagg auguuaaaaa guuguaccac ucagaagccu      840

ucacugucaa cuucggggac accgaagagg ccaagaaaca gaucaacgau uacguggaga      900

aggguacuca agggaaaauu guggauuugg ucaaggagcu ugacagagac acaguuuuug      960

cucuggugaa uuacaucuuc uuuaaaggca aaugggagag acccuuugaa gucaaggaca     1020

ccgaggaaga ggacuuccac guggaccagg ugaccaccgu gaaggugccu augaugaagc     1080

guuuaggcau guuuaacauc cagcacugua agaagcuguc cagcugggug cugcugauga     1140

aauaccuggg caaugccacc gccaucuucu uccugccuga ugaggggaaa cuacagcacc     1200

uggaaaauga acucacccac gauaucauca ccaaguuccu ggaaaaugaa gacagaaggu     1260

cugccagcuu acauuuaccc aaacugucca uuacuggaac cuaugaucug aagagcgucc     1320

ugggucaacu gggcaucacu aaggucuuca gcaauggggc ugaccucucc ggggucacag     1380

aggaggcacc ccugaagcuc uccaaggccg ugcauaaggc ugugcugacc aucgacgaga     1440

aagggacuga agcugcuggg gccauguuuu uagaggccau acccaugucu aucccccccg     1500

aggucaaguu caacaaaccc uuugucuucu uaaugauuga acaaaauacc aagucucccc     1560

ucuucauggg aaaaguggug aaucccaccc aaaaauaacu gccucucgcu ccucaacccc     1620

uccccuccau cccuggcccc cucccuggau gacauuaaag aaggguugag cuggucccug     1680

ccugcaugug acuguaaauc ccucccaugu uuucucugag ucucccuuug ccugcugagg     1740

cuguaugugg gcuccaggua acagugcugu cuucgggccc ccugaacugu guucauggag     1800

caucuggcug gguaggcaca ugcugggcuu gaauccaggg gggacugaau ccucagcuua     1860

cggaccuggg cccaucuguu ucuggagggc uccagucuuc cuuguccugu cuuggagucc     1920

ccaagaagga aucacagggg aggaaccaga uaccagccau gaccccaggc uccaccaagc     1980

aucuucaugu cccccugcuc aucccccacu cccccccacc cagaguugcu cauccugcca     2040

gggcuggcug ugcccacccc aaggcugccc uccugggggc cccagaacug ccugaucgug     2100

ccguggccca guuuuguggc aucugcagca acacaagaga gaggacaaug uccuccucuu     2160

gacccgcugu caccuaacca gacucgggcc cugcaccucu caggcacuuc uggaaaauga     2220

cugaggcaga uucuuccuga agcccauucu ccauggggca acaaggacac cuauucuguc     2280

cuuguccuuc caucgcugcc ccagaaagcc ucacauaucu ccguuuagaa ucaggucccu     2340

ucuccccaga ugaagaggag ggucucugcu uuguuuucuc uaucuccucc ucagacuuga     2400

ccaggcccag caggccccag aagaccauua cccuauaucc cuucuccucc cuagucacau     2460

ggccauaggc cugcugaugg cucaggaagg ccauugcaag gacuccucag cuaugggaga     2520

ggaagcacau cacccauuga cccccgcaac cccucccuuu ccuccucuga gucccgacug     2580

gggccacaug cagccugacu ucuuugugcc uguugcuguc ccugcagucu ucagagggcc     2640

accgcagcuc cagugccacg gcaggaggcu guuccugaau agccccugug guaagggcca     2700

ggagaguccu uccauccucc aaggcccugc uaaaggacac agcagccagg aaguccccug     2760

ggccccuagc ugaaggacag ccugcucccu ccgucucuac caggaauggc cuuguccuau     2820

ggaaggcacu gccccauccc aaacuaaucu aggaaucacu gucuaaccac ucacugucau     2880

gaauguguac uuaaaggaug agguugaguc auaccaaaua gugauuucga uaguucaaaa     2940

uggugaaauu agcaauucua caugauucag ucuaaucaau ggauaccgac uguuucccac     3000

acaagucucc uguucucuua agcuuacuca cugacagccu uucacucucc acaaauacau     3060

uaaagauaug gccaucacca agcccccuag gaugacacca gaccugagag ucugaagacc     3120

uggauccaag uucugacuuu ucccccugac agcuguguga ccuucgugaa gucgccaaac     3180

cucucugagc cccagucauu gcuaguaaga ccugccuuug aguugguaug auguucaagu     3240

uagauaacaa aauguuuaua cccauuagaa cagagaauaa auagaacuac auuucuugca     3300


