                               SEQUENCE LISTING

<110> SYNTHEGO CORPORATION
 
<120> METHODS AND SYSTEMS FOR GUIDE RNA DESIGN AND USE

<130> 54108-708.601

<140> PCT/US2019/032735
<141> 2019-05-16

<150> 62/672,437
<151> 2018-05-16

<160> 91    

<170> PatentIn version 3.5

<210> 1
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 1
aggccugggc uggcucugcc                                                   20


<210> 2
<211> 20
<212> DNA
<213> Homo sapiens

<400> 2
aggcctgggc tggctctgcc                                                   20


<210> 3
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 3
uccccuggca gagccagccc                                                   20


<210> 4
<211> 20
<212> DNA
<213> Homo sapiens

<400> 4
tcccctggca gagccagccc                                                   20


<210> 5
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 5
uaggggccag aggccugggc                                                   20


<210> 6
<211> 20
<212> DNA
<213> Homo sapiens

<400> 6
taggggccag aggcctgggc                                                   20


<210> 7
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 7
cacauagggg ccagaggccu                                                   20


<210> 8
<211> 20
<212> DNA
<213> Homo sapiens

<400> 8
cacatagggg ccagaggcct                                                   20


<210> 9
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 9
tggcgcagtg ctgaccttga                                                   20


<210> 10
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 10
ggcgcagtgc tgaccttgat                                                   20


<210> 11
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 11
cgcagtgctg accttgatgg                                                   20


<210> 12
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 12
gcagtgctga ccttgatggt                                                   20


<210> 13
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 13
cagtgctgac cttgatggtg                                                   20


<210> 14
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 14
tgctgacctt gatggtgggg                                                   20


<210> 15
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 15
gctgaccttg atggtggggt                                                   20


<210> 16
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 16
cttgatggtg gggtgggtct                                                   20


<210> 17
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 17
tgatggtggg gtgggtcttg                                                   20


<210> 18
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 18
gatggtgggg tgggtcttgg                                                   20


<210> 19
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 19
gtatctgtgc tcctctcgcc                                                   20


<210> 20
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 20
tatctgtgct cctctcgcct                                                   20


<210> 21
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 21
ctcgcctggg atgctgcccg                                                   20


<210> 22
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 22
gggatgctgc ccgcggagcg                                                   20


<210> 23
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 23
gcggagcgcc cctcgcactt                                                   20


<210> 24
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 24
cggagcgccc ctcgcacttg                                                   20


<210> 25
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 25
agcgcccctc gcacttgtag                                                   20


<210> 26
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 26
ctcgcacttg tagcggaagc                                                   20


<210> 27
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 27
tcgcacttgt agcggaagcg                                                   20


<210> 28
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 28
cgcacttgta gcggaagcgc                                                   20


<210> 29
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 29
gaagcgcatg ccccgctgct                                                   20


<210> 30
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 30
aagcgcatgc cccgctgctt                                                   20


<210> 31
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 31
cgctgcttgg gctgctcaat                                                   20


<210> 32
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 32
gctgcttggg ctgctcaatg                                                   20


<210> 33
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 33
ctgcttgggc tgctcaatga                                                   20


<210> 34
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 34
ctgctcaatg atctccacat                                                   20


<210> 35
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 35
tgctcaatga tctccacata                                                   20


<210> 36
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 36
gctcaatgat ctccacatag                                                   20


<210> 37
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 37
gatctccaca taggggccag                                                   20


<210> 38
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 38
ccacataggg gccagaggcc                                                   20


<210> 39
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 39
cataggggcc agaggcctgg                                                   20


<210> 40
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 40
gaggcctggg ctggctctgc                                                   20


<210> 41
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 41
ggcctgggct ggctctgcca                                                   20


<210> 42
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 42
gcctgggctg gctctgccag                                                   20


<210> 43
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 43
gggctggctc tgccagggga                                                   20


<210> 44
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 44
ggggacaccg cagccccatt                                                   20


<210> 45
<211> 20
<212> DNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 45
gacaccgcag ccccattagg                                                   20


<210> 46
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 46
uccccuggca gaggcagccc                                                   20


<210> 47
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 47
gaucuccaca uaggggccag                                                   20


<210> 48
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 48
ccaggccucu ggccccuaug                                                   20


<210> 49
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 49
gcucaaugau cuccacauag                                                   20


<210> 50
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 50
gauggugggg ugggucuugg                                                   20


<210> 51
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 51
gcugaccuug augguggggu                                                   20


<210> 52
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 52
ugcugaccuu gauggugggg                                                   20


<210> 53
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 53
cagugcugac cuugauggug                                                   20


<210> 54
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 54
gcagugcuga ccuugauggu                                                   20


<210> 55
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 55
cgcagugcug accuugaugg                                                   20


<210> 56
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 56
caagacccac cccaccauca                                                   20


<210> 57
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 57
gacaccgcag ccccauuagg                                                   20


<210> 58
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 58
ggggacaccg cagccccauu                                                   20


<210> 59
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 59
aauggggcug cggugucccc                                                   20


<210> 60
<211> 20
<212> RNA
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      oligonucleotide

<400> 60
uccccuggca gagccagccc                                                   20


<210> 61
<211> 30
<212> DNA
<213> Homo sapiens

<400> 61
aggcctgggc tggctctgcc aggggacacc                                        30


<210> 62
<211> 30
<212> DNA
<213> Homo sapiens

<400> 62
ggtgtcccct ggcagagcca gcccaggcct                                        30


<210> 63
<211> 20
<212> DNA
<213> Homo sapiens

<400> 63
ttctctggca gagccagccc                                                   20


<210> 64
<211> 20
<212> DNA
<213> Homo sapiens

<400> 64
caccctggca gagccagccc                                                   20


<210> 65
<211> 20
<212> DNA
<213> Homo sapiens

<400> 65
tcccctggca gaggcagcgc                                                   20


<210> 66
<211> 20
<212> DNA
<213> Homo sapiens

<400> 66
tccccaggca gaagcagccc                                                   20


<210> 67
<211> 20
<212> DNA
<213> Homo sapiens

<400> 67
tggcctggca aagccagccc                                                   20


<210> 68
<211> 20
<212> DNA
<213> Homo sapiens

<400> 68
tcccctgaaa cagccagccc                                                   20


<210> 69
<211> 20
<212> DNA
<213> Homo sapiens

<400> 69
tcccctggca gaggcgcccc                                                   20


<210> 70
<211> 20
<212> DNA
<213> Homo sapiens

<400> 70
ttcccaggca gagccagcca                                                   20


<210> 71
<211> 20
<212> DNA
<213> Homo sapiens

<400> 71
tccccaggcc cagccagccc                                                   20


<210> 72
<211> 20
<212> DNA
<213> Homo sapiens

<400> 72
tcccctggca gagcctggca                                                   20


<210> 73
<211> 20
<212> DNA
<213> Homo sapiens

<400> 73
ggcactggca gagccagccc                                                   20


<210> 74
<211> 20
<212> DNA
<213> Homo sapiens

<400> 74
tcccctggca gagaaagcac                                                   20


<210> 75
<211> 20
<212> DNA
<213> Homo sapiens

<400> 75
gcccttggaa gagccagccc                                                   20


<210> 76
<211> 20
<212> DNA
<213> Homo sapiens

<400> 76
ttccctggct gagcaagccc                                                   20


<210> 77
<211> 20
<212> DNA
<213> Homo sapiens

<400> 77
tccccaggca gagccggcct                                                   20


<210> 78
<211> 20
<212> DNA
<213> Homo sapiens

<400> 78
tcccctctca gtgccagccc                                                   20


<210> 79
<211> 20
<212> DNA
<213> Homo sapiens

<400> 79
gcccctggca gtgcaagccc                                                   20


<210> 80
<211> 20
<212> DNA
<213> Homo sapiens

<400> 80
tccccaggca gagcctgcca                                                   20


<210> 81
<211> 20
<212> DNA
<213> Homo sapiens

<400> 81
tccctgggca gagccagctc                                                   20


<210> 82
<211> 20
<212> DNA
<213> Homo sapiens

<400> 82
gctcctggca gtgccagccc                                                   20


<210> 83
<211> 20
<212> DNA
<213> Homo sapiens

<400> 83
tcccgtggca gggtcagccc                                                   20


<210> 84
<211> 20
<212> DNA
<213> Homo sapiens

<400> 84
tcccctggca ggccctgccc                                                   20


<210> 85
<211> 20
<212> DNA
<213> Homo sapiens

<400> 85
tcccctgtga gtgccagccc                                                   20


<210> 86
<211> 20
<212> DNA
<213> Homo sapiens

<400> 86
tcccctggta gggctagccc                                                   20


<210> 87
<211> 20
<212> DNA
<213> Homo sapiens

<400> 87
tccccgacca gagccagccc                                                   20


<210> 88
<211> 20
<212> DNA
<213> Homo sapiens

<400> 88
tcctctgaca gggccagccc                                                   20


<210> 89
<211> 20
<212> DNA
<213> Homo sapiens

<400> 89
tgccctggca aggccagccc                                                   20


<210> 90
<211> 20
<212> DNA
<213> Homo sapiens

<400> 90
tcacccagca gagccagccc                                                   20


<210> 91
<211> 6
<212> PRT
<213> Artificial Sequence

<220>
<223> Description of Artificial Sequence: Synthetic
      6xHis tag

<400> 91
His His His His His His 
1               5       


