                         SEQUENCE LISTING

<110>  UNIVERSITE DE STRASBOURG
       CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE
 
<120>  ABSOLUTE QUANTIFICATION OF TARGET MOLECULES AT SINGLE-ENTITY 
       RESOLUTION USING TANDEM BARCODING

<130>  B2754

<160>  39    

<170>  PatentIn version 3.5

<210>  1
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template UEI-DraIII

<400>  1
gagcggataa caatttcaca caggcacggg gtgtgagatc acagatcgga agagcgtcgt       60

gt                                                                      62


<210>  2
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UEI-Fwd

<400>  2
gagcggataa caatttcaca cagg                                              24


<210>  3
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  UEI-Rev

<400>  3
acacgacgct cttccgatc                                                    19


<210>  4
<211>  142
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template Calibrator-N15-AlwNI


<220>
<221>  misc_feature
<222>  (86)..(110)
<223>  n is a, c, g, or t

<400>  4
tcgtcggcag cgtcagatgt gtataagaga cagggaagac gtagcaaggg agtagccaat       60

gtgaattgag agccttaagc tgtatnnnnn nnnnnnnnnn nnnnnnnnnn caggggctgg      120

tcgtgactgg gaaaaccctg gc                                               142


<210>  5
<211>  23
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Calib-Rev

<400>  5
gccagggttt tcccagtcac gac                                               23


<210>  6
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illu-2-Fwd

<400>  6
tcgtcggcag cgtcagatg                                                    19


<210>  7
<211>  67
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template UEI-N15-DraIII*


<220>
<221>  misc_feature
<222>  (34)..(48)
<223>  n is a, c, g, or t

<400>  7
gagcggataa caatttcaca caggcacggg gtgnnnnnnn nnnnnnnnga tcggaagagc       60

gtcgtgt                                                                 67


<210>  8
<211>  149
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template Calibrator-N15-AlwNI-lg


<220>
<221>  misc_feature
<222>  (103)..(117)
<223>  n is a, c, g, or t

<400>  8
tcgtcggcag cgtcagatgt gtataagaga cagggaagac gtagcaaggg agtagccaat       60

gtgaattgag agccttaagc tgtatcagga ccagagagat gannnnnnnn nnnnnnncag      120

gggctggtcg tgactgggaa aaccctggc                                        149


<210>  9
<211>  53
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illu1 rev UEI

<400>  9
gtctcgtggg ctcggagatg tgtataagag acagacacga cgctcttccg atc              53


<210>  10
<211>  19
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Illu1

<400>  10
gtctcgtggg ctcggagat                                                    19


<210>  11
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template-AlwNI-N15-Calib-DraIII


<220>
<221>  misc_feature
<222>  (44)..(58)
<223>  n is a, c, g, or t

<400>  11
ggaagacgta gcaagggagt agccaatgtg aattcacaca gtgnnnnnnn nnnnnnnnca       60

ggggctggtc gtgactggga aaaccctggc                                        90


<210>  12
<211>  24
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Calib-Fwd

<400>  12
ggaagacgta gcaagggagt agcc                                              24


<210>  13
<211>  80
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RTmimicks


<220>
<221>  misc_feature
<222>  (36)..(43)
<223>  n is a, c, g, or t

<400>  13
ctgcgcggat cccggaagcg aggccagctg gctgcnnnnn nnnttggctg tctcttatac       60

acatctgacg ctgccgacga                                                   80


<210>  14
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  antiSBACA-AlwN1

<400>  14
gcagccagct ggcctcgctt ccgggatccg cgcagaca                               38


<210>  15
<211>  101
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template UEI-N15-DraIII-Illu1


<220>
<221>  misc_feature
<222>  (34)..(48)
<223>  n is a, c, g, or t

<400>  15
gagcggataa caatttcaca caggcacggg gtgnnnnnnn nnnnnnnnga tcggaagagc       60

gtcgtgtctg tctcttatac acatctccga gcccacgaga c                          101


<210>  16
<211>  539
<212>  RNA
<213>  Staphylococcus aureus

<400>  16
auuaauacga cucacuauac cuagaucaca gagaugugau ggaaaauagu ugaugaguug       60

uuuaauuuua agaauuuuua ucuuaauuaa ggaaggagug auuucaaugg cacaagauau      120

cauuucaaca aucggugacu uaguaaaaug gauuaucgac acagugaaca aauucacuaa      180

aaaauaagau gaauaauuaa uuacuuucau uguaaauuug uuaucuacgu auaguacuaa      240

aaguaugagu uauuaagcca ucccaacuua auaaccaugu aaaauuagca agugaguaac      300

auuugcuagu agaguuaguu uccuuggacu cagugcuaug uauuuuucuu aauuaucauu      360

acagauaauu auuucuagca uguaagcuau cguaaacaac aucgauuuau cauuauuuga      420

uaaauaaaau uuuuuucaua auuaauaaca uccccaaaaa uagauugaaa aaauaacugu      480

aaaacauucc cuuaauaaua aguauggucg ugagccccuc ccaagcucgc ggccuuuug       539


<210>  17
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  empty


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  n is a, c, g, or t

<400>  17
nnnnnnnnnn                                                              10


<210>  18
<211>  55
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RT-UMI-RNAIII*


<220>
<221>  misc_feature
<222>  (36)..(41)
<223>  n is a, c, g, or t

<400>  18
ctgcgcggat cccggaagcg aggccagctg gctgcnnnnn nttgggaggg gctca            55


<210>  19
<211>  10
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  empty


<220>
<221>  misc_feature
<222>  (1)..(10)
<223>  n is a, c, g, or t

<400>  19
nnnnnnnnnn                                                              10


<210>  20
<211>  35
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  SB-alone

<400>  20
ctgcgcggat cccggaagcg aggccagctg gctgc                                  35


<210>  21
<211>  63
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNAIII-Fwd

<400>  21
tcgtcggcag cgtcagatgt gtataagaga cagataactg taaaacattc ccttaataat       60

aag                                                                     63


<210>  22
<211>  867
<212>  RNA
<213>  Artificial Sequence

<220>
<223>  Gfp mRNA

<400>  22
augaccagcu acccauacga uguuccagau uacgcuaucg aaggccgcgg ccgccaucau       60

caucaucauc augauaucgg uaccaguaaa ggagaagaac uuuucacugg aguuguccca      120

auucuuguug aauuagaugg ugauguuaau gggcacaaau uuucugucag uggagagggu      180

gaaggugaug caacauacgg aaaacuuacc cuuaaauuua uuugcacuac uggaaaacua      240

ccuguuccau ggccaacacu ugucacuacu uucgcguaug gucuucaaug cuuugcgaga      300

uacccagauc auaugaaaca gcaugacuuu uucaagagug ccaugcccga agguuaugua      360

caggaaagaa cuauauuuuu caaagaugac gggaacuaca agacacgugc ugaagucaag      420

uuugaaggug auacccuugu uaauagaauc gaguuaaaag guauugauuu uaaagaagau      480

ggaaacauuc uuggacacaa auuggaauac aacuauaacu cacacaaugu auacaucaug      540

gcagacaaac aaaagaaugg aaucaaaguu aacuucaaaa uuagacacaa cauugaagau      600

ggaagcguuc aacuagcaga ccauuaucaa caaaauacuc caauuggcga uggcccuguc      660

cuuuuaccag acaaccauua ccuguccaca caaucugccc uuucgaaaga ucccaacgaa      720

aagagagacc acaugguccu ucuugaguuu guaacagcug cugggauuac acauggcaug      780

gaugaacuau acaaagagaa uucagagcuc ggauccacuc gagaugcauu agaacaaaaa      840

uuauuaucag aagaagauuu aaauuaa                                          867


<210>  23
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  gfp-mut2-RT-UMI-AlwNI*


<220>
<221>  misc_feature
<222>  (36)..(43)
<223>  n is a, c, g, or t

<400>  23
ctgcgcggat cccggaagcg aggccagctg gctgcnnnnn nnncaaataa atttaagggt       60

aagttttcc                                                               69


<210>  24
<211>  26
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Gfp-Fwd-25nt

<400>  24
tgaattagat ggtgatgtta atgggc                                            26


<210>  25
<211>  70
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template-UEI-DraIII-N2x5


<220>
<221>  misc_feature
<222>  (34)..(35)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (38)..(39)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (42)..(43)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (46)..(47)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (50)..(51)
<223>  n is a, c, g, or t

<400>  25
gagcggataa caatttcaca caggcacggg gtgnnacnng annctnngcn ngatcggaag       60

agcgtcgtgt                                                              70


<210>  26
<211>  76
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template-UEI-DraIII-N4443


<220>
<221>  misc_feature
<222>  (34)..(37)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (41)..(44)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (48)..(51)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (55)..(57)
<223>  n is a, c, g, or t

<400>  26
gagcggataa caatttcaca caggcacggg gtgnnnnaca nnnngagnnn ntctnnngat       60

cggaagagcg tcgtgt                                                       76


<210>  27
<211>  85
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template-Calibrator-AlwNI

<400>  27
ggaagacgta gcaagggagt agccaatgtg aattcacaca gtgtctacaa gtacaggggc       60

tggtcgtgac tgggaaaacc ctggc                                             85


<210>  28
<211>  93
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template-Calibrator-AlwNI-N2x5


<220>
<221>  misc_feature
<222>  (44)..(45)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (48)..(49)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (52)..(53)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (56)..(57)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (60)..(61)
<223>  n is a, c, g, or t

<400>  28
ggaagacgta gcaagggagt agccaatgtg aattcacaca gtgnntcnnt anncannagn       60

ncaggggctg gtcgtgactg ggaaaaccct ggc                                    93


<210>  29
<211>  99
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template-Calibrator-AlwNI-N4443


<220>
<221>  misc_feature
<222>  (44)..(47)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (51)..(54)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (58)..(61)
<223>  n is a, c, g, or t

<220>
<221>  misc_feature
<222>  (65)..(67)
<223>  n is a, c, g, or t

<400>  29
ggaagacgta gcaagggagt agccaatgtg aattcacaca gtgnnnntct nnnnacannn       60

ngagnnncag gggctggtcg tgactgggaa aaccctggc                              99


<210>  30
<211>  90
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template-Calibrator-AlwNI-N15


<220>
<221>  misc_feature
<222>  (44)..(58)
<223>  n is a, c, g, or t

<400>  30
ggaagacgta gcaagggagt agccaatgtg aattcacaca gtgnnnnnnn nnnnnnnnca       60

ggggctggtc gtgactggga aaaccctggc                                        90


<210>  31
<211>  100
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Tag-NaBAb-AlwNI


<220>
<221>  misc_feature
<222>  (32)..(39)
<223>  n is a, c, g, or t

<400>  31
cacacaggaa acagctatga cccagtgtct gnnnnnnnnt gtggacgctg tctcttatac       60

acatctgacg ctgccgacga cgtcgtgact gggaaaaccc                            100


<210>  32
<211>  20
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  BG-M13-Fwd**

<400>  32
gggttttccc agtcacgacg                                                   20


<210>  33
<211>  22
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Alexa488-M13-Rev

<400>  33
cacacaggaa acagctatga cc                                                22


<210>  34
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Example of UEI sequence


<220>
<221>  misc_feature
<222>  (34)..(43)
<223>  n is a, c, g, or t

<400>  34
gagcggataa caatttcaca caggcacggg gtgnnnnnnn nnngatcgga agagcgtcgt       60

gt                                                                      62


<210>  35
<211>  62
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  example of UEI sequence


<220>
<221>  misc_feature
<222>  (20)..(29)
<223>  n is a, c, g, or t

<400>  35
acacgacgct cttccgatcn nnnnnnnnnc accccgtgcc tgtgtgaaat tgttatccgc       60

tc                                                                      62


<210>  36
<211>  83
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  Template UEI-N15-DraIII-Illu2


<220>
<221>  misc_feature
<222>  (34)..(48)
<223>  n is a, c, g, or t

<400>  36
gagcggataa caatttcaca caggcacggg gtgnnnnnnn nnnnnnnnaa ctgtctctta       60

tacacatctg acgctgccga cga                                               83


<210>  37
<211>  66
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  RNAIII-Fw-89-Lg

<400>  37
gtctcgtggg ctcggagatg tgtataagag acagtcataa ttaataacat ccccaaaaat       60

agattg                                                                  66


<210>  38
<211>  38
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of Figure 22

<400>  38
gcagccagct ggcctcgctt ccgggatccg cgcagaca                               38


<210>  39
<211>  69
<212>  DNA
<213>  Artificial Sequence

<220>
<223>  sequence of Figure 22


<220>
<221>  misc_feature
<222>  (36)..(43)
<223>  n is a, c, g, or t

<400>  39
ctgcgcggat cccggaagcg aggccagctg gctgcnnnnn nnncaaataa atttaagggt       60

aagttttcc                                                               69


