               SEQUENCE LISTING

<110> MolMed SpA
      Glycostem Therapeutics B.V.

<120> Manufacturing Process

<130> IP-7

<150> EP18175055
<151> 2018-05-30

<160> 14

<170> BiSSAP 1.3.6

<210> 1
<211> 222
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence spacer domain (NWL)

<400> 1
Lys Glu Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys 
1               5                   10                  15      
Lys Ala Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn 
            20                  25                  30          
Gln Thr Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val 
        35                  40                  45              
Val Ser Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu 
    50                  55                  60                  
Gln Ser Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg 
65                  70                  75                  80  
Cys Ala Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala 
                85                  90                  95      
Cys Arg Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp 
            100                 105                 110         
Lys Gln Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp 
        115                 120                 125             
Glu Ala Asn His Val Asp Pro Cys Leu Pro Cys Thr Val Cys Glu Asp 
    130                 135                 140                 
Thr Glu Arg Gln Leu Arg Glu Cys Thr Arg Trp Ala Asp Ala Glu Cys 
145                 150                 155                 160 
Glu Glu Ile Pro Gly Arg Trp Ile Thr Arg Ser Thr Pro Pro Glu Gly 
                165                 170                 175     
Ser Asp Ser Thr Ala Pro Ser Thr Gln Glu Pro Glu Ala Pro Pro Glu 
            180                 185                 190         
Gln Asp Leu Ile Ala Ser Thr Val Ala Gly Val Val Thr Thr Val Met 
        195                 200                 205             
Gly Ser Ser Gln Pro Val Val Thr Arg Gly Thr Thr Asp Asn 
    210                 215                 220         

<210> 2
<211> 666
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleotide sequence spacer domain (NWL)

<400> 2
aaagaggcct gccccaccgg cctgtacacc cacagcggag agtgctgcaa ggcctgcaac      60

ctgggagagg gcgtggccca gccttgcggc gccaatcaga ccgtgtgcga gccctgcctg     120

gacagcgtga ccttcagcga cgtggtgtcc gccaccgagc cctgcaagcc ttgcaccgag     180

tgtgtgggcc tgcagagcat gagcgccccc tgcgtggaag ccgacgacgc cgtgtgtaga     240

tgcgcctacg gctactacca ggacgagaca accggcagat gcgaggcctg tagagtgtgc     300

gaggccggca gcggcctggt gttcagttgt caagacaagc agaataccgt gtgtgaagag     360

tgccccgacg gcacctacag cgacgaggcc aaccacgtgg acccctgcct gccctgcact     420

gtgtgcgagg acaccgagcg gcagctgcgc gagtgcacaa gatgggccga cgccgagtgc     480

gaagagatcc ccggcagatg gatcaccaga agcacccccc ctgagggcag cgacagcacc     540

gcccctagca cccaggaacc tgaggcccct cccgagcagg acctgatcgc ctctacagtg     600

gccggcgtgg tgacaaccgt gatgggcagc tctcagcccg tggtgacacg gggcaccacc     660

gacaat                                                                666


<210> 3
<211> 162
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence spacer domain (NWS)

<400> 3
Lys Glu Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys 
1               5                   10                  15      
Lys Ala Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn 
            20                  25                  30          
Gln Thr Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val 
        35                  40                  45              
Val Ser Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu 
    50                  55                  60                  
Gln Ser Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg 
65                  70                  75                  80  
Cys Ala Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala 
                85                  90                  95      
Cys Arg Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp 
            100                 105                 110         
Lys Gln Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp 
        115                 120                 125             
Glu Ala Asn His Val Asp Pro Cys Leu Pro Cys Thr Val Cys Glu Asp 
    130                 135                 140                 
Thr Glu Arg Gln Leu Arg Glu Cys Thr Arg Trp Ala Asp Ala Glu Cys 
145                 150                 155                 160 
Glu Glu 
        

<210> 4
<211> 486
<212> DNA
<213> Artificial sequence


<220> 
<223> Nucleotide sequence spacer domain (NWS)

<400> 4
aaagaggcct gccccaccgg cctgtacacc cacagcggag agtgctgcaa ggcctgcaac      60

ctgggagagg gcgtggccca gccttgcggc gccaatcaga ccgtgtgcga gccctgcctg     120

gacagcgtga ccttcagcga cgtggtgtcc gccaccgagc cctgcaagcc ttgcaccgag     180

tgtgtgggcc tgcagagcat gagcgccccc tgcgtggaag ccgacgacgc cgtgtgtaga     240

tgcgcctacg gctactacca ggacgagaca accggcagat gcgaggcctg tagagtgtgc     300

gaggccggca gcggcctggt gttcagttgt caggacaagc agaacaccgt gtgtgaagag     360

tgccccgacg gcacctacag cgacgaggcc aaccacgtgg acccctgcct gccctgcact     420

gtgtgcgagg acaccgagcg gcagctgcgc gagtgcacaa gatgggccga cgccgagtgc     480

gaggaa                                                                486


<210> 5
<211> 200
<212> PRT
<213> Artificial sequence


<220> 
<223> Protein sequence spacer domain (NML)

<400> 5
Lys Glu Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys 
1               5                   10                  15      
Lys Ala Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn 
            20                  25                  30          
Gln Thr Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val 
        35                  40                  45              
Val Ser Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu 
    50                  55                  60                  
Gln Ser Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg 
65                  70                  75                  80  
Cys Ala Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala 
                85                  90                  95      
Cys Arg Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp 
            100                 105                 110         
Lys Gln Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp 
        115                 120                 125             
Glu Ala Ala Arg Ala Ala Asp Ala Glu Cys Glu Glu Ile Pro Gly Arg 
    130                 135                 140                 
Trp Ile Thr Arg Ser Thr Pro Pro Glu Gly Ser Asp Ser Thr Ala Pro 
145                 150                 155                 160 
Ser Thr Gln Glu Pro Glu Ala Pro Pro Glu Gln Asp Leu Ile Ala Ser 
                165                 170                 175     
Thr Val Ala Gly Val Val Thr Thr Val Met Gly Ser Ser Gln Pro Val 
            180                 185                 190         
Val Thr Arg Gly Thr Thr Asp Asn 
        195                 200 

<210> 6
<211> 600
<212> DNA
<213> Artificial sequence


<220> 
<223> Nucleotide sequence spacer domain (NML)

<400> 6
aaagaggcct gccccaccgg cctgtacacc cacagcggag agtgctgcaa ggcctgcaac      60

ctgggagagg gcgtggccca gccttgcggc gccaatcaga ccgtgtgcga gccctgcctg     120

gacagcgtga ccttcagcga cgtggtgtcc gccaccgagc cctgcaagcc ttgcaccgag     180

tgtgtgggcc tgcagagcat gagcgccccc tgcgtggaag ccgacgacgc cgtgtgtaga     240

tgcgcctacg gctactacca ggacgagaca accggcagat gcgaggcctg tagagtgtgc     300

gaggccggca gcggcctggt gttcagttgt caagacaagc agaataccgt gtgtgaagag     360

tgccccgacg gcacctacag cgacgaagcc gccagagccg ccgacgccga gtgcgaagag     420

atccccggca gatggatcac cagaagcacc ccccctgagg gcagcgacag caccgcccct     480

agcacccagg aacctgaggc ccctcccgag caggacctga tcgcctctac agtggccggc     540

gtggtgacaa ccgtgatggg cagctctcag cccgtggtga cacggggcac caccgacaat     600


<210> 7
<211> 140
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence spacer domain (NMS)

<400> 7
Lys Glu Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys 
1               5                   10                  15      
Lys Ala Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn 
            20                  25                  30          
Gln Thr Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val 
        35                  40                  45              
Val Ser Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu 
    50                  55                  60                  
Gln Ser Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg 
65                  70                  75                  80  
Cys Ala Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala 
                85                  90                  95      
Cys Arg Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp 
            100                 105                 110         
Lys Gln Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp 
        115                 120                 125             
Glu Ala Ala Arg Ala Ala Asp Ala Glu Cys Glu Glu 
    130                 135                 140 

<210> 8
<211> 420
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleotide sequence spacer domain (NMS)

<400> 8
aaagaggcct gccccaccgg cctgtacacc cacagcggag agtgctgcaa ggcctgcaac      60

ctgggagagg gcgtggccca gccttgcggc gccaatcaga ccgtgtgcga gccctgcctg     120

gacagcgtga ccttcagcga cgtggtgtcc gccaccgagc cctgcaagcc ttgcaccgag     180

tgtgtgggcc tgcagagcat gagcgccccc tgcgtggaag ccgacgacgc cgtgtgtaga     240

tgcgcctacg gctactacca ggacgagaca accggcagat gcgaggcctg tagagtgtgc     300

gaggccggca gcggcctggt gttcagttgt caggacaagc agaacaccgt gtgtgaagag     360

tgccccgacg gcacctacag cgacgaggcc gcccgggccg ccgacgccga gtgcgaggaa     420


<210> 9
<211> 27
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence transmembrane domain of CD28

<400> 9
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val 
            20                  25          

<210> 10
<211> 68
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence transmembrane and intracellular portion of the
      human CD28

<400> 10
Phe Trp Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu 
1               5                   10                  15      
Leu Val Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser 
            20                  25                  30          
Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly 
        35                  40                  45              
Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala 
    50                  55                  60                  
Ala Tyr Arg Ser 
65              

<210> 11
<211> 41
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence intracellular domain of CD28

<400> 11
Arg Ser Lys Arg Ser Arg Leu Leu His Ser Asp Tyr Met Asn Met Thr 
1               5                   10                  15      
Pro Arg Arg Pro Gly Pro Thr Arg Lys His Tyr Gln Pro Tyr Ala Pro 
            20                  25                  30          
Pro Arg Asp Phe Ala Ala Tyr Arg Ser 
        35                  40      

<210> 12
<211> 113
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence signaling domain of human CD3 zeta chain

<400> 12
Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln Gln Gly 
1               5                   10                  15      
Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu Glu Tyr 
            20                  25                  30          
Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly Gly Lys 
        35                  40                  45              
Pro Gln Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu Gln 
    50                  55                  60                  
Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly Glu 
65                  70                  75                  80  
Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser Thr 
                85                  90                  95      
Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro Pro 
            100                 105                 110         
Arg 
    

<210> 13
<211> 1131
<212> DNA
<213> Artificial Sequence


<220> 
<223> Nucleotide sequence suicide gene is the HSV-TK Mut2

<400> 13
atggcttcgt acccctgcca tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc      60

ggccatagca accgacgtac ggcgttgcgc cctcgccggc agcaagaagc cacggaagtc     120

cgcctggagc agaaaatgcc cacgctactg cgggtttata tagacggtcc tcacgggatg     180

gggaaaacca ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac     240

gtacccgagc cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc     300

tacaccacac aacaccgcct cgaccagggc gagatatcgg ccggggacgc ggcggtggta     360

atgacaagcg cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct     420

cctcatgtcg ggggggaggc tgggagttca catgccccgc ccccggccct caccctcatc     480

ttcgaccgcc atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc     540

agcatgaccc cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc     600

acaaacatcg tgttgggggc ccttccggag gacagacaca tcgaccgcct ggccaaacgc     660

cagcgccccg gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg     720

ctgcttgcca atacggtgcg gtatctgcag ggcggcgggt cgtggtggga ggattgggga     780

cagctttcgg ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca     840

cgaccccata tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc     900

aacggcgacc tgtataacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt     960

cccatgcacg tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg    1020

ctgcaactta cctccgggat ggtccagacc cacgtcacca ccccaggctc cataccgacg    1080

atctgcgacc tggcgcgcac gtttgcccgg gagatggggg aggctaactg a             1131


<210> 14
<211> 658
<212> PRT
<213> Artificial Sequence


<220> 
<223> Protein sequence CAR CD44v6-NWL

<400> 14
Met Glu Ala Pro Ala Gln Leu Leu Phe Leu Leu Leu Leu Trp Leu Pro 
1               5                   10                  15      
Asp Thr Thr Gly Glu Ile Val Leu Thr Gln Ser Pro Ala Thr Leu Ser 
            20                  25                  30          
Leu Ser Pro Gly Glu Arg Ala Thr Leu Ser Cys Ser Ala Ser Ser Ser 
        35                  40                  45              
Ile Asn Tyr Ile Tyr Trp Leu Gln Gln Lys Pro Gly Gln Ala Pro Arg 
    50                  55                  60                  
Ile Leu Ile Tyr Leu Thr Ser Asn Leu Ala Ser Gly Val Pro Ala Arg 
65                  70                  75                  80  
Phe Ser Gly Ser Gly Ser Gly Thr Asp Phe Thr Leu Thr Ile Ser Ser 
                85                  90                  95      
Leu Glu Pro Glu Asp Phe Ala Val Tyr Tyr Cys Leu Gln Trp Ser Ser 
            100                 105                 110         
Asn Pro Leu Thr Phe Gly Gly Gly Thr Lys Val Glu Ile Lys Arg Gly 
        115                 120                 125             
Gly Gly Gly Ser Gly Gly Gly Gly Ser Glu Val Gln Leu Val Glu Ser 
    130                 135                 140                 
Gly Gly Gly Leu Val Lys Pro Gly Gly Ser Leu Arg Leu Ser Cys Ala 
145                 150                 155                 160 
Ala Ser Gly Phe Thr Phe Ser Ser Tyr Asp Met Ser Trp Val Arg Gln 
                165                 170                 175     
Ala Pro Gly Lys Gly Leu Glu Trp Val Ser Thr Ile Ser Ser Gly Gly 
            180                 185                 190         
Ser Tyr Thr Tyr Tyr Leu Asp Ser Ile Lys Gly Arg Phe Thr Ile Ser 
        195                 200                 205             
Arg Asp Asn Ala Lys Asn Ser Leu Tyr Leu Gln Met Asn Ser Leu Arg 
    210                 215                 220                 
Ala Glu Asp Thr Ala Val Tyr Tyr Cys Ala Arg Gln Gly Leu Asp Tyr 
225                 230                 235                 240 
Trp Gly Arg Gly Thr Leu Val Thr Val Ser Ser Gly Asp Pro Lys Glu 
                245                 250                 255     
Ala Cys Pro Thr Gly Leu Tyr Thr His Ser Gly Glu Cys Cys Lys Ala 
            260                 265                 270         
Cys Asn Leu Gly Glu Gly Val Ala Gln Pro Cys Gly Ala Asn Gln Thr 
        275                 280                 285             
Val Cys Glu Pro Cys Leu Asp Ser Val Thr Phe Ser Asp Val Val Ser 
    290                 295                 300                 
Ala Thr Glu Pro Cys Lys Pro Cys Thr Glu Cys Val Gly Leu Gln Ser 
305                 310                 315                 320 
Met Ser Ala Pro Cys Val Glu Ala Asp Asp Ala Val Cys Arg Cys Ala 
                325                 330                 335     
Tyr Gly Tyr Tyr Gln Asp Glu Thr Thr Gly Arg Cys Glu Ala Cys Arg 
            340                 345                 350         
Val Cys Glu Ala Gly Ser Gly Leu Val Phe Ser Cys Gln Asp Lys Gln 
        355                 360                 365             
Asn Thr Val Cys Glu Glu Cys Pro Asp Gly Thr Tyr Ser Asp Glu Ala 
    370                 375                 380                 
Asn His Val Asp Pro Cys Leu Pro Cys Thr Val Cys Glu Asp Thr Glu 
385                 390                 395                 400 
Arg Gln Leu Arg Glu Cys Thr Arg Trp Ala Asp Ala Glu Cys Glu Glu 
                405                 410                 415     
Ile Pro Gly Arg Trp Ile Thr Arg Ser Thr Pro Pro Glu Gly Ser Asp 
            420                 425                 430         
Ser Thr Ala Pro Ser Thr Gln Glu Pro Glu Ala Pro Pro Glu Gln Asp 
        435                 440                 445             
Leu Ile Ala Ser Thr Val Ala Gly Val Val Thr Thr Val Met Gly Ser 
    450                 455                 460                 
Ser Gln Pro Val Val Thr Arg Gly Thr Thr Asp Asn Pro Lys Phe Trp 
465                 470                 475                 480 
Val Leu Val Val Val Gly Gly Val Leu Ala Cys Tyr Ser Leu Leu Val 
                485                 490                 495     
Thr Val Ala Phe Ile Ile Phe Trp Val Arg Ser Lys Arg Ser Arg Leu 
            500                 505                 510         
Leu His Ser Asp Tyr Met Asn Met Thr Pro Arg Arg Pro Gly Pro Thr 
        515                 520                 525             
Arg Lys His Tyr Gln Pro Tyr Ala Pro Pro Arg Asp Phe Ala Ala Tyr 
    530                 535                 540                 
Arg Ser Arg Val Lys Phe Ser Arg Ser Ala Asp Ala Pro Ala Tyr Gln 
545                 550                 555                 560 
Gln Gly Gln Asn Gln Leu Tyr Asn Glu Leu Asn Leu Gly Arg Arg Glu 
                565                 570                 575     
Glu Tyr Asp Val Leu Asp Lys Arg Arg Gly Arg Asp Pro Glu Met Gly 
            580                 585                 590         
Gly Lys Pro Arg Arg Lys Asn Pro Gln Glu Gly Leu Tyr Asn Glu Leu 
        595                 600                 605             
Gln Lys Asp Lys Met Ala Glu Ala Tyr Ser Glu Ile Gly Met Lys Gly 
    610                 615                 620                 
Glu Arg Arg Arg Gly Lys Gly His Asp Gly Leu Tyr Gln Gly Leu Ser 
625                 630                 635                 640 
Thr Ala Thr Lys Asp Thr Tyr Asp Ala Leu His Met Gln Ala Leu Pro 
                645                 650                 655     
Pro Arg 
        

